Study on Microbiome of Supragingival Plaque of College Students with Gingivitis
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摘要:
目的 采用高通量测序技术比较牙龈健康大学生和牙龈炎大学生龈上菌斑微生物差异。 方法 按照纳入、排除标准，在昆明医科大学招募18～25岁牙龈健康大学生30名、牙龈炎大学生33名，采用MiSeq系统对16S rDNA基因扩增子进行测序，比较2组受试者龈上菌斑的多样性、结构和组成。 结果 共检测到87，595个序列，其中包含14个门216个属。与牙龈健康组相比，牙龈炎组受试者的龈上菌斑中α多样性指标较高，差异具有统计学意义（P < 0.001）。相似性分析（Anosim）和主成分分析（PCA）显示牙龈健康组和牙龈炎组受试者的龈上菌斑微生物群落结构、组成存在差异，且差异具有统计学意义（P < 0.001）。2组受试者龈上菌斑群落的相对丰度存在差异，牙龈炎组中普氏菌属、卟啉单胞菌属、杆菌属的相对丰度较高，而牙龈健康组中放线菌属的相对丰度较高。 结论 牙龈炎组和牙龈健康组大学生受试者龈上菌斑的微生物群落组成和结构存在差异，牙龈炎组龈上菌斑的多样性较高。 Abstract:Objective To compare the microbial differences of supragingival plaque between healthy gingiva group and gingivitis group of college students by high-throughput sequencing technology. Methods According to inclusion and exclusion criteria, 30 college students aged 18-25 with healthy gingiva and 33 with gingivitis were recruited from Kunming Medical University. Sequencing of 16S rDNA gene amplicon was performed using MiSeq system, and the diversity, structure and composition of supragingival plaque were compared between the two groups. Results A total of 87, 595 sequences were detected, including 14 phyla and 216 genera. Compared with the healthy gingiva group, the α diversity index in the gingivitis group was higher, and the difference was statistically significant （P < 0.001）. The result of similarity analysis （Anosim） and principal component analysis （PCA） showed that there were significant differences in the structure and composition of the microflora of supragingival plaque between the healthy gingiva group and the gingivitis group （P < 0.001）. The relative abundance of Prevotella, Porphyromonas and Bacillus in gingivitis group was higher, while the relative abundance of Actinomycetes in healthy gingiva group was higher. Conclusion There are significant differences in microbial community composition and structure of supragingival plaque between the healthy gingiva group and the gingivitis group of college students, and the diversity of the gingivitis group is higher. -
Key words:
- Gingivitis /
- High-throughput sequencing /
- Microbiome /
- College students
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图 1 牙龈健康组（NO）龈上菌斑物种丰度、多样性低于牙龈炎组（CO）
A：健康组与牙龈炎组间的物种总数的散点分布即丰度（richness）；B：Shannon指数比较图；***P < 0.001。
Figure 1. The species abundance and diversity of plaque in gingivitis group （NO） were lower than that in gingivitis group （CO）
图 2 基于OTU丰度的主成分分析（PCoA）
Figure 2. Principal Component Analysis （PCoA） based on OTU abundance
图 3 门水平健康组（NO）与牙龈炎组（CO）的物种相对丰度
该菌门在NO组中水平显著较高，*P < 0.05。
Figure 3. Relative species abundance of the healthy group （NO） and the gingivitis group （CO） at phylum level
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