胸主动脉阻断联合主动脉旁路循环技术建立大鼠脊髓缺血损伤模型

杨克; 费俊杰; 段玉印; 李鑫; 吴忠诚; 黄莹山

doi:10.12259/j.issn.2095-610X.S20220217

胸主动脉阻断联合主动脉旁路循环技术建立大鼠脊髓缺血损伤模型

doi: 10.12259/j.issn.2095-610X.S20220217

1.
云南省阜外心血管病医院麻醉科，云南昆明　650032
2.
四川省成都市第三人民医院心脏大血管外科，四川成都　610000
3.
云南省阜外心血管病医院心外科，云南昆明　650032

基金项目: 云南省应用基础研究基金资助项目（2017FE468-192）

详细信息

作者简介:
杨克（1981～），男，山西霍州人，硕士，主要从事麻醉学、围术期器官保护研究工作

通讯作者:
段玉印，E-mail：duanyuyin@kmmu.edu.cn

中图分类号: R744.1
计量
- 文章访问数: 3444
- HTML全文浏览量: 2118
- PDF下载量: 19
- 被引次数: 0
出版历程
- 收稿日期: 2021-12-07
- 网络出版日期: 2022-02-24
- 刊出日期: 2022-03-04

Establishment of Spinal Cord Ischemia Injury Model in Rats by Thoracic Aorta Occlusion Combined with Aortic Bypass

1.
Dept. of Anesthesiology，Yunnan Fuwai Cardiovascular Hospital，Kunming Yunnan 650032
2.
Dept. of Cardio-Vascular Surgery，The Third People’s Hospital of Chengdu，Chengdu Sichuan 610000
3.
Dept. of Cardiology，Yunnan Fuwai Cardiovascular Hospital， Kunming Yunnan 650032，China

摘要

摘要: 目的改良大鼠脊髓缺血损伤动物模型。方法大鼠分为3组：实验组，阻断胸主动脉（T4-T11）+主动脉旁路循环（胸主动脉起始部-胸主动脉近膈肌部），阻断20 min；对照组，阻断胸主动脉（T4）20 min；假手术组，只开胸不阻断胸主动脉。术中采用经颅运动诱发电位（Tc-MEP）监测脊髓功能，术后7 d观察脊髓切片病理改变；采用BBB评分评估脊髓功能，28 d分析大鼠生存曲线。结果实验组Tc-MEP波形6 min后消失，对照组8 min后Tc-MEP波形消失，直至手术结束。脊髓切片显示实验组神经元大量变性坏死，对照组多个神经元坏死。实验组比对照组BBB评分下降明显；28 d生存率差异有统计学意义（P < 0.05）。结论胸主动脉阻断+主动脉旁路循环方法，对比传统的胸主动脉阻断方法，效果确切，存活率高。
- 脊髓缺血损伤 /
- 动物模型 /
- 主动脉旁路循环 /
- 主动脉阻断
Abstract: Objective To modify the rat model of spinal cord ischemia injury. Methods The rats were divided into three groups. In experimental group, thoracic aorta was cross-clamped between T4 and T11, and aortic bypass circulation was established from proximal thoracic aorta to proximal diaphragmatic aorta. The interval of clamping was 20 minutes. In control group, the thoracic aorta （T4） was cross-clamped for 20 minutes.In the sham operation group, only thoracotomy was performed without thoracic aorta cross-clamping. Thefunction of spinal cord was monitored by transcranial motor evoked potential （TC-MEP）. The pathology of spinal cord were observed 7 days after surgery. The locomotor function of spinal cord was evaluated by BBB score at multiple postoperative time. The survival curves were analyzed 28 days after operation. Results The TC-MEP waveform of the experimental group disappeared after 6 minutes, and that of the control group disappeared after 8 minutes until the end of surgery. The slices of spinal cord showed a large number of degeneration and necrosis of neurons in the experimental group. Multiple neurons were necrotic in the control group. The BBB score of the experimental group decreased more obviously than that of the control group. There was a significant difference in the 28-day survival rate between the experimental group and the control group, and （P < 0.05）. Conclusion Thoracic aortic cross-clamping plus aortic bypass is an effective method, with high long-term survival rate.
- Spinal cord ischemia injury /
- Animal model /
- Aortic bypass /
- Aortic cross-clamping

HTML全文

图 1 胸主动脉阻断+主动脉旁路循环示意图

在胸主动脉阻断期间，旁路管道向远端主动脉供血。

Figure 1. Schematic diagram of thoracic aorta cross-clamping + aortic bypass

下载: 全尺寸图片幻灯片

图 2 在胸主动脉阻断后Tc-MEP波形消失，表明脊髓功能损伤。

Figure 2. The TC-MEP waveform disappeared after thoracic aorta cross-clamping，indicating impaired spinal function.

下载: 全尺寸图片幻灯片

图 3 BBB运动评分折线图，实验组与对照组比较，差异有统计学意义。

^*P < 0.05，^**P < 0.01，^***P < 0.001，^****P < 0.0001。

Figure 3. Line chart of BBB locomotor scores of rats. There was significant difference between treatment and controlgroup.

下载: 全尺寸图片幻灯片

图 4 术后7 d大鼠胸段脊髓的组织学改变（HE染色）（×200）

A：假手术组，组织学正常；B：对照组，神经元数量减少，炎症细胞浸润；C：实验组，少量神经元存活，炎性细胞浸润。

Figure 4. Histological changes of the thoracic spinal cord in rats 7 days after surgery （HE staining）（×200）

下载: 全尺寸图片幻灯片

图 5 28 d生存率比较

Kaplan-Meier生存曲线，Log Rank检验，实验组与对照组有显著性差异，^*P = 0.0412。

Figure 5. Comparison of 28-day overall survival rate （Kaplan-Meier survivalcurve，Log Rank test）

下载: 全尺寸图片幻灯片

参考文献(12)

[1]	Sharif-Alhoseini M,Khormali M,Rezaei M,et al. Animal models of spinal cord injury:a systematic review[J]. Spinal Cord.,2017,55(8):714-721. doi: 10.1038/sc.2016.187
[2]	Ryu J H,Park J W,Hwang J Y,et al. The attenuation of neurological injury from the use of simvastatin after spinal cord ischemia-reperfusion injury in rats[J]. BMC Anesthesiol,2018,27,18(1):31.
[3]	Dong Q,Sun L,Peng L,et al. PMX53 protects spinal cord from ischemia-reperfusion injury in rats in the short term[J]. Spinal Cord,2016,54(4):254-258. doi: 10.1038/sc.2015.146
[4]	Tamaki T, Kubota S. History of the development of intraoperative spinal cord monitoring[J]. Eur Spine J, 2007 , 16 （Suppl 2）: S140-146.
[5]	Basso D M,Beattie M S,Bresnahan J C. A sensitive and reliable locomotor rating scale for open field testing in rats[J]. J Neurotrauma.,1995,12(1):1-21. doi: 10.1089/neu.1995.12.1
[6]	Chen F,Wang D,Jiang Y,et al. Dexmedetomidine Postconditioning alleviates Spinal Cord Ischemia-reperfusion Injury in Rats via Inhibiting Neutrophil Infiltration,Microglia Activation,Reactive Gliosis and CXCL13/CXCR5 Axis Activation[J]. Int J Neurosci,2021,27:1-20.
[7]	Mazensky D,Flesarova S,Sulla I. Arterial blood supply to the spinal cord in animal models of spinal cord injury. A review[J]. Anat Rec (Hoboken),2017,300(12):2091-2106. doi: 10.1002/ar.23694
[8]	Bell M T,Reece T B,Smith P D,et al. Reproducable paraplegia by thoracic aortic occlusion in a murine model of spinal cord ischemia-reperfusion[J]. J Vis Exp,2014,85:50910.
[9]	Marsala M,Yaksh T L. Transient spinal ischemia in the rat:characterization of behavioral and histopathological consequences as a function of the duration of aortic occlusion[J]. J Cereb Blood Flow Metab,1994,14(3):526-535. doi: 10.1038/jcbfm.1994.65
[10]	Aydemir S,Dogan D,Kocak A,et al. The effect of melatonin on spinal cord after ischemia in rats[J]. Spinal Cord,2016,54(5):360-363. doi: 10.1038/sc.2015.204
[11]	Gong S,Seng Z,Wang W,et al. Bosentan protects the spinal cord from ischemia reperfusion injury in rats through vascular endothelial growth factor receptors[J]. Spinal Cord,2015,53(1):19-23. doi: 10.1038/sc.2014.147
[12]	Carrillo S E,Guimarães S B,Vasconcelos P R,et al. Is subdiaphragmatic aortic cross-clamping a suitable model for spinal cord ischemia/reperfusion injury study in rats?[J]. Acta Cir Bras,2006,21(4):219-222.

相关文章(20)

[1]	字正梅, 郝丽, 王南, 陈国兵. 联合预测模型对急性A型主动脉夹层破裂的诊断价值, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20230128
[2]	杨琳娟, 金文娇, 陆金芝, 熊正花, 闫斌, 韩雪松. 不同方式构建宫腔粘连大鼠模型的比较, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20230322
[3]	杨镕羽, 宋飞, 黄浩, 段开文, 钱石兵, 向盈盈. SD大鼠慢性根尖周炎动物模型的研究进展, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20221005
[4]	杨秀强, 张晓帆, 任玉玲, 李娟娟, 张利伟. OCT评估磁性微珠注射诱导小鼠慢性高眼压模型的建立, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20201245
[5]	曹倩, 李兰, 邹莹, 李云川, 李勇, 龙俊君, 刘浩文, 何柳余, 田二苗. 心脏死亡大鼠供体角膜移植排斥模型建立, 昆明医科大学学报.
[6]	任旭, 王沂峰, 戴丽冰. 子宫内膜异位症SCID小鼠动物模型的构建, 昆明医科大学学报.
[7]	曹贵华. 滇南小耳猪原位肾移植模型的建立, 昆明医科大学学报.
[8]	吴剑. 前降支结扎构建滇南小耳猪心梗诱导的缺血性心肌病模型, 昆明医科大学学报.
[9]	奎泽宏. 建立家兔梗阻性无精症模型对显微外科培训的实验性探讨, 昆明医科大学学报.
[10]	吴京蔓. 舌鳞癌Cal27裸鼠移植瘤模型的建立及其意义, 昆明医科大学学报.
[11]	张浒. 二尖瓣狭窄动物模型的建立, 昆明医科大学学报.
[12]	光雪峰. 左向右分流型肺动脉高压动物模型的制作, 昆明医科大学学报.
[13]	陈锦润. 3,4-苯并芘支气管灌注构建猪肺癌模型的实验研究, 昆明医科大学学报.
[14]	陈郊丽. 糖尿病鼠模型研究新进展, 昆明医科大学学报.
[15]	闫永吉. 肾上腺醛固酮腺瘤大鼠模型的建立, 昆明医科大学学报.
[16]	柳爱华. 雪胆素对实验性肝损伤的保护作用, 昆明医科大学学报.
[17]	. 人免疫重建NOD/SCID小鼠模型的建立, 昆明医科大学学报.
[18]	. 大鼠变应性鼻炎模型中T-bet/GATA-3基因表达的研究, 昆明医科大学学报.
[19]	纳钊. 日本大耳兔胆管电灼损伤致瘢痕狭窄模型的建立, 昆明医科大学学报.
[20]	刁畅. 不阻断体循环及门脉系统大鼠胰十二指肠移植模型的建立, 昆明医科大学学报.