Optimization of Heating Reflux Extraction Process for Qingyangshengenin
-
摘要:
目的 考察药用植物青阳参根茎中青阳参苷元的最佳提取工艺。 方法 利用加热回流法提取、HPLC法检测青阳参苷元，在单因素实验基础上，通过正交设计法研究乙醇体积分数、料液比、提取时间对青阳参苷元提取效果的影响。 结果 最佳提取工艺条件为乙醇体积分数70%、料液比1∶15（g/mL）、提取时间2 h，验证得到青阳参苷元提取率可达0.3090%。 结论 经过优化的青阳参苷元加热回流提取工艺有效、稳定、绿色，为进一步开展青阳参苷元药理活性、结构修饰等研究提供基础。 Abstract:Objective To investigate the optimum extraction technology of Qingyangshengenin from the medicinal plant Cynanchum otophyllum Schnei. Methods The reflux method is used to extract and HPLC method is used to detect Qingyangshengenin. On the basis of single factor experimental, orthogonal design method is selected to explore the conditions of ethanol volume fraction, solid-liquid ratio and extraction time on the extraction of Qingyangshengenin. Results The optimal extraction conditions were as follows: ethanol volume fraction 70%, solid-liquid ratio 1∶15（g/mL）, extraction time 2 h, and the extraction rate was up to 0.3090%. Conclusion The optimized heating reflux extraction process was effective, stable and environment-friendly, which provided a foundation for further studies on pharmacological activity and structural modification of Qingyangshengenin. -
Key words:
- Cynanchum otophyllum Schnei /
- Qingyangshengenin /
- Reflux extraction /
- 0rthogonal design.
-
图 2 乙醇体积分数对青阳参苷元提取率的影响
Figure 2. Effect of ethanol volume fraction on extraction rate of Qingyangshengenin
图 3 料液比对青阳参苷元提取率的影响
Figure 3. Effect of solid-liquid ratio on extraction rate of Qingyangshengenin
表 1 盐酸浓度对青阳参苷元提取率的影响
Table 1. Effect of Hydrochloric acid concentrationon extraction rate of Qingyangshengenin
序号 盐酸浓度（mol/L） 青阳参苷元提取率（%） 1 0.10 0.2144 2 0.12 0.2209 3 0.14 0.2239 4 0.16 0.2404 5 0.18 0.2237 6 0.20 0.2323 
下载: 导出CSV
表 2 因素水平表
Table 2. The table of factor and level
因素 X-乙醇体积分数（%） Y-料液比（g/mL） Z-提取时间（h） 1 50 1∶15 1 2 70 1∶10 2 3 90 1∶5 3
下载: 导出CSV
表 3 不同提取条件下青阳参苷元的提取率
Table 3. The extraction rate of Qingyangshengenin under various extraction conditions
实验序号 因素 提取率（%） X-乙醇体积分数（%） Y-料液比（g/mL） Z-提取时间（h） 1 1 1 1 0.2828 2 1 2 2 0.2809 3 1 3 3 0.2631 4 2 1 2 0.3041 5 2 2 3 0.3017 6 2 3 1 0.2839 7 3 1 3 0.2956 8 3 2 1 0.2944 9 3 3 2 0.2870 ${\rm{ \overline K1}}$ 0.2756 0.2941 0.2870 ${\rm{ \overline K2}} $ 0.2965 0.2923 0.2906 ${\rm{ \overline K3}} $ 0.2923 0.2780 0.2868 $R $ 0.0210 0.0162 0.0039
下载: 导出CSV
表 4 方差分析结果
Table 4. The result of variance analysis
方差来源 离差平方和 自由度 均方 F P X 0.0738 2 0.0369 29.8111 0.0325* Y 0.0470 2 0.0235 19.0043 0.0500 Z 0.0028 2 0.0014 1.1401 0.4673 误差 0.0025 2 0.0012 *P < 0.05。
下载: 导出CSV
-
[1] 黎丽云,殷志琦,张庆文. 云南民间药青阳参的研究进展[J]. 海峡药学,2011,23(4):1-5. doi: 10.3969/j.issn.1006-3765.2011.04.001 [2] 杨明, 李云峰, 王鲁明. 青阳参甙（苷）元和包含它的提取物的医药用途[P].中国: CN 101953864. B, 2013-05-22. [3] 龙霞,戴逢春,肖小华. 青阳参总甙对癫痫发作后脑损伤的神经保护作用的研究[J]. 吉林医学,2014,35(19):4135-4137. [4] 张萌,高博,李勇辉. 青阳参总苷对社会挫败应激模型大鼠的抗抑郁作用研究[J]. 中国民族民间医药,2018,27(15):17-20. [5] Gu X J,Hao D C. Recent advances in phytochemistry and pharmacology of C21 steroid constituents from Cynanchum plants[J]. Chin J Nat Med,2016,14(5):321-334. [6] Li X S,Yang X S,Liu L. Three new steroidal sapogenins derived from the roots of Cynanchum otophyllum and their cytotoxic activities[J]. Phytochemistry Letters,2021,45:105-109. doi: 10.1016/j.phytol.2021.08.002 [7] Dong J R,Peng X R,Li L. C21 steroidal glycosides with cytotoxic activities from Cynanchum otophyllum[J]. Bioorganic & Medicinal Chemistry Letters,2018,28(9):1520-1524. [8] Zhan Z J,Bao S M,Zhang Y. New immunomodulating polyhydroxypregnane glycosides from the roots of Cynanchum otophyllum[J]. Chemistry & Biodiversity,2019,16(6):1900062. [9] Ma X X,Jiang F T,Yang Q X. New pregnane glycosides from the roots of Cynanchum otophyllum[J]. Steroids,2007,72(11-12):778-786. doi: 10.1016/j.steroids.2007.06.001 [10] 刘云怀,黄云先,木书林. 青阳参质量标准的改进研究[J]. 中国卫生标准管理,2015,25(6):130-134. [11] 张琳,李晓誉,许世芳. 双波长HPLC测定青阳参中青阳参苷元和告达亭的含量[J]. 中国现代应用药学,2014,31(3):317-319. [12] 周进康,何军,梁志远. 青阳参皂苷超声提取工艺的研究[J]. 时珍国医国药,2010,21(2):416-417. doi: 10.3969/j.issn.1008-0805.2010.02.076 [13] 钱一鑫, 李魏林, 何珺. 青阳参分散片及其制备方法[P].中国: CN 101357149 B, 2011-06-29. [14] 钱一鑫, 康冀川, 何珺. 复方青阳参口服制剂及其制备方法[P].中国: CN 105998119 B, .2019-12-31. [15] Li X S,Yang X S,Ding W J. New C21-steroidal aglycones from the roots of Cynanchum otophyllum and their anticancer[J]. Fitoterapia,2021,149(5):104833. [16] Dong J R,Yue G L G,Lee M K J. Potential neurotrophic activity and cytotoxicity of selected C21 steroidal glycosides from Cynanchum otophyllum[J]. Medicinal Chemistry Research,2020,29(3):549-555. doi: 10.1007/s00044-020-02506-7 -
点击查看大图
计量
- 文章访问数: 2100
- HTML全文浏览量: 1931
- PDF下载量: 111
- 被引次数: 0
