Optimization of Heating Reflux Extraction Process for Qingyangshengenin
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摘要:
目的 考察药用植物青阳参根茎中青阳参苷元的最佳提取工艺。 方法 利用加热回流法提取、HPLC法检测青阳参苷元，在单因素实验基础上，通过正交设计法研究乙醇体积分数、料液比、提取时间对青阳参苷元提取效果的影响。 结果 最佳提取工艺条件为乙醇体积分数70%、料液比1∶15（g/mL）、提取时间2 h，验证得到青阳参苷元提取率可达0.3090%。 结论 经过优化的青阳参苷元加热回流提取工艺有效、稳定、绿色，为进一步开展青阳参苷元药理活性、结构修饰等研究提供基础。 Abstract:Objective To investigate the optimum extraction technology of Qingyangshengenin from the medicinal plant Cynanchum otophyllum Schnei. Methods The reflux method is used to extract and HPLC method is used to detect Qingyangshengenin. On the basis of single factor experimental, orthogonal design method is selected to explore the conditions of ethanol volume fraction, solid-liquid ratio and extraction time on the extraction of Qingyangshengenin. Results The optimal extraction conditions were as follows: ethanol volume fraction 70%, solid-liquid ratio 1∶15（g/mL）, extraction time 2 h, and the extraction rate was up to 0.3090%. Conclusion The optimized heating reflux extraction process was effective, stable and environment-friendly, which provided a foundation for further studies on pharmacological activity and structural modification of Qingyangshengenin. -
Key words:
- Cynanchum otophyllum Schnei /
- Qingyangshengenin /
- Reflux extraction /
- 0rthogonal design.
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图 2 乙醇体积分数对青阳参苷元提取率的影响
Figure 2. Effect of ethanol volume fraction on extraction rate of Qingyangshengenin
图 3 料液比对青阳参苷元提取率的影响
Figure 3. Effect of solid-liquid ratio on extraction rate of Qingyangshengenin
表 1 盐酸浓度对青阳参苷元提取率的影响
Table 1. Effect of Hydrochloric acid concentrationon extraction rate of Qingyangshengenin
序号 盐酸浓度（mol/L） 青阳参苷元提取率（%） 1 0.10 0.2144 2 0.12 0.2209 3 0.14 0.2239 4 0.16 0.2404 5 0.18 0.2237 6 0.20 0.2323 
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表 2 因素水平表
Table 2. The table of factor and level
因素 X-乙醇体积分数（%） Y-料液比（g/mL） Z-提取时间（h） 1 50 1∶15 1 2 70 1∶10 2 3 90 1∶5 3
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表 3 不同提取条件下青阳参苷元的提取率
Table 3. The extraction rate of Qingyangshengenin under various extraction conditions
实验序号 因素 提取率（%） X-乙醇体积分数（%） Y-料液比（g/mL） Z-提取时间（h） 1 1 1 1 0.2828 2 1 2 2 0.2809 3 1 3 3 0.2631 4 2 1 2 0.3041 5 2 2 3 0.3017 6 2 3 1 0.2839 7 3 1 3 0.2956 8 3 2 1 0.2944 9 3 3 2 0.2870 ${\rm{ \overline K1}}$ 0.2756 0.2941 0.2870 ${\rm{ \overline K2}} $ 0.2965 0.2923 0.2906 ${\rm{ \overline K3}} $ 0.2923 0.2780 0.2868 $R $ 0.0210 0.0162 0.0039
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表 4 方差分析结果
Table 4. The result of variance analysis
方差来源 离差平方和 自由度 均方 F P X 0.0738 2 0.0369 29.8111 0.0325* Y 0.0470 2 0.0235 19.0043 0.0500 Z 0.0028 2 0.0014 1.1401 0.4673 误差 0.0025 2 0.0012 *P < 0.05。
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