木犀草素通过调控内质网应激抑制平滑肌细胞的迁移

张黎; 王清岑; 周罗慧; 杨娟; 王红

doi:10.12259/j.issn.2095-610X.S20220518

木犀草素通过调控内质网应激抑制平滑肌细胞的迁移

doi: 10.12259/j.issn.2095-610X.S20220518

张黎¹,
王清岑²,
周罗慧¹,
杨娟¹,
王红^1, ,

1.
昆明医科大学附属心血管病医院十七病区，云南昆明　650500
2.
攀枝花市中西医结合医院心内科，四川攀枝花　617000

基金项目: 云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目[2019FE001（-280）]，云南省阜外心血管病医院心力衰竭诊疗省创新团队基金资助项目[201905E160013]

详细信息

作者简介:
张黎（1986～），男，云南大理人，医学硕士，主治医师，主要从事心血管疾病诊治及心血管疾病相关基础研究工作

王清岑与张黎对本文有同等贡献

通讯作者:
王红，E-mail：wangh43@126.com

中图分类号: R541.4
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出版历程
- 收稿日期: 2021-11-05
- 网络出版日期: 2022-05-12
- 刊出日期: 2022-05-27

Luteolin Inhibits HASMCs Migration by Endoplasmic Reticulum Stress

1.
Ward Seventeen of Cardiovascular Medicine，The Affiliated Cardiovascular Hospital of Kunming Medical University，Kunming Yunnan 650500
2.
Dept. of Cardiology， Panzhihua Integrated Traditional Chinese and Western Medicine Hospital， Panzhihua Sichuan 617000，China

摘要

摘要: 目的研究木犀草素对人主动脉平滑肌细胞迁移的调控作用，并探讨其调控作用是否通过激活内质网应激途径实现。方法体外培养人主动脉平滑肌细胞，分为空白对照组（不做任何处理，BC）、阴性对照组（加等量无关试剂，NC）、木犀草素组（25 μmol/L处理）、木犀草素+4-苯基丁酸钠共处理组（4-苯基丁酸钠预处理后加入等量木犀草素），于12h小时后细胞划痕实验法检测人主动脉平滑肌细胞迁移情况，Western blot和RT-qPCR检测BIP、CHOP蛋白及RNA表达水平。结果（1）与空白对照组和阴性对照组相比，木犀草素组细胞迁移水平明显降低，BIP、CHOP表达水平明显增高；（2）与木犀草素组相比，木犀草素+4-苯基丁酸钠共处理组细胞迁移水平增强，同时BIP、CHOP表达水平降低。结论木犀草素通过上调内质网应激抑制人主动脉平滑肌细胞的迁移。
- 木犀草素 /
- 内质网应激 /
- 人主动脉平滑肌细胞 /
- 迁移
Abstract: Objective To investigate the regulatory effect and mechanism of luteolin on the migration of human aortic smooth muscle cells （HASMCs）. Methods HASMCs were cultured in vitro and divided into the blank control group （without any treatment, BC）, the negative control group （with the same amount of irrelevant reagent, NC）, the luteolin group （25 μmol/L treatment, LUT） and the luteolin+4-PBA treatment group （with the same amout of luteolin added after 4-PBA pretreatment, 4-PBA）. Cell scratch test was used to detect the HASMCs migration after 12hs. The expression level of BIP and CHOP about endoplasmic reticulum stress were detected by RT-qPCR and Western blot. Results （1） Compared to the BC and NC groups, the cell migration level in the LUT group was significantly reduced, and the expression levels of BIP and CHOP were significantly increased. （2） Compared with the LUT group, the cell migration level of the luteolin+4-PBA co-teatment group increased, while the expression levels of BIP and CHOP decreased. Conclusion Luteolin inhibits HASMCs migration by promoting the endoplasmic reticulum stress.
- Luteolin /
- Endoplasmic Reticulum stress /
- Human aortic smooth muscle cells /
- Migration

HTML全文

图 1 木犀草素对HASMCs迁移率的影响

与0 μM组比较，^*P < 0.05。

Figure 1. Effects of luteolin on migration rate of HASMCs

下载: 全尺寸图片幻灯片

图 2 木犀草素对HASMCs内质网应激的影响

A：空白对照组、阴性对照组、木犀草素组和木犀草素+4-PBA共处理组处理12 h进行Western Blot实验后BIP、CHOP、β-actin蛋白显影情况；B：Western Blot检测木犀草素组内质网应激相关蛋白BIP表达水平；C：Western Blot检测木犀草素组内质网应激相关蛋白CHOP表达水平；D： RT-qPCR检测木犀草素组内质网应激相关基因BIP表达水平；E：RT-qPCR检测木犀草素组内质网应激相关基因CHOP表达水平与空白对照组及阴性对照组比较，^**P < 0.01；与木犀草+4-PBA处理组比较，^*P < 0.05。

Figure 2. Effects of luteolin on endoplasmic reticulum stress in HASMCs

下载: 全尺寸图片幻灯片

图 3 细胞划痕实验检测HASMCs迁移水平

与对照组比较，^**P < 0.01；与木犀草素组比较，^*P < 0.05。

Figure 3. HASMCs migration was detected by cell scratch assay

下载: 全尺寸图片幻灯片

表 1 BIP、CHOP、GAPDH引物序列

Table 1. BIP、CHOP、GAPDH primer sequence

名称	正向引物序列	反向引物序列
BIP	-TCTACTATGAAGCCCGTCCAGA -	-GCTTCATCTGGGTTTATGCCAC -
CHOP	-ATCTTCACCACTCTTGACCCTG-	-ACTCTGTTTCCGTTTCCTGGTT-
GAPDH	-GAAGGTCGGAGTCAACGGATTT-	-GCCATGGGTGGAATCATATTGG-

下载: 导出CSV

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