赫特维希上皮根鞘细胞的研究进展

毕菲; 郭维华

doi:10.12259/j.issn.2095-610X.S20220628

赫特维希上皮根鞘细胞的研究进展

doi: 10.12259/j.issn.2095-610X.S20220628

毕菲^{1, 2},
郭维华^{1, 2, ,}

1.
四川大学华西口腔医院儿童口腔科，四川成都　610041
2.
口腔疾病研究国家重点实验室，四川成都　610041

基金项目: 国家自然科学基金资助项目（31971281）；四川省科技创新人才项目（2022JDRC0043）；四川大学华西口腔医院探索与研发项目（RD-03-202106）

详细信息

作者简介:
毕菲（1995～），女，云南昆明人，在读硕士研究生，主要从事儿童口腔早期矫治临床工作及牙源性干细胞相关基础研究工作

通讯作者:
郭维华，E-mail：guoweihua943019@163.com

中图分类号: R78
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- 文章访问数: 2039
- HTML全文浏览量: 1665
- PDF下载量: 31
- 被引次数: 0
出版历程
- 收稿日期: 2022-03-28
- 网络出版日期: 2022-06-08
- 刊出日期: 2022-06-30

Advances in Research on Hertwig’s Epithelial Root Sheath Cells

Fei BI^{1, 2},
Weihua GUO^{1, 2
, ,}

1.
Dept. of Pediatric Dentistry，West China School of Stomatology，Sichuan University，Chengdu，China 610041
2.
State Key Laboratory of Oral Diseases，West China Hospital of Stomatology，Sichuan University，Chengdu 610041，China

摘要

摘要: 赫特维希上皮根鞘（Hertwig’s epithelial root sheath, HERS）细胞是一群在牙根发育以及牙周组织形成过程中发挥重要作用的特殊细胞。就HERS细胞起源、形态结构，以及近年来关于其生物学行为特点、参与牙发育调控过程、应用于组织修复再生潜力的研究作一综述，为后续HERS细胞及其衍生物在牙周组织再生中的应用策略和机制探究提供参考。
- 赫特维希上皮根鞘细胞 /
- 牙根发育 /
- 上皮-间充质转化 /
- 上皮-间充质相互作用 /
- 牙周组织再生
Abstract: Hertwig's epithelial root sheath （HERS） cells are a special group of cells that play important roles during root development and formation of periodontal tissues. This paper summarizes the origin and morphological structure of HERS cells, as well as the recent researches on their biological behaviors, participation in the regulatory process of tooth development, and potential application to tissue repair and regeneration. It provides a reference for the subsequent study on application strategies and mechanisms of HERS cells and their derivatives in periodontal tissue regeneration.
- Hertwig’s epithelial root sheath cells /
- tooth root development /
- epithelial-mesenchymal transition /
- epithelial-mesenchymal interaction /
- periodontal tissue regeneration

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参考文献(69)

[1]	于世凤. 口腔组织病理学[M]. 北京: 人民卫生出版社, 2012: 40-44.
[2]	Slavkin H C,Bringas P J,Bessem C,et al. Hertwig’s epithelial root sheath differentiation and initial cementum and bone formation during long-term organ culture of mouse mandibular first molars using serumless,chemically-defined medium[J]. J Periodontal Res,1989,24(1):28-40. doi: 10.1111/j.1600-0765.1989.tb00854.x
[3]	Luan X,Ito Y,Diekwisch T G. Evolution and development of Hertwig’s epithelial root sheath[J]. Dev Dyn,2006,235(5):1167-1180. doi: 10.1002/dvdy.20674
[4]	Howes R J. Root formation in ectopically transplanted teeth of the frog,Rana pipiens I. Tooth morphogenesis[J]. Acta Anat (Basel),1977,97(2):151-165. doi: 10.1159/000144729
[5]	Suzuki M,Inoue T,Shimono M,et al. Behavior of epithelial root sheath during tooth root formation in porcine molars:TUNEL,TEM,and immunohistochemical studies[J]. Anat Embryol (Berl),2002,206(1-2):13-20. doi: 10.1007/s00429-002-0276-3
[6]	Hamamoto Y,Nakajima T,Ozawa H. Ultrastructural and histochemical study on the morphogenesis of epithelial rests of Malassez[J]. Arch Histol Cytol,1989,52(1):61-70. doi: 10.1679/aohc.52.61
[7]	Andujar M B,Magloire H,Hartmann D J,et al. Early mouse molar root development:cellular changes and distribution of fibronectin,laminin and type-IV collagen[J]. Differentiation,1985,30(2):111-122. doi: 10.1111/j.1432-0436.1985.tb00522.x
[8]	Mu H,Liu X,Geng S,et al. Epithelial Bone Morphogenic Protein 2 and 4 Are Indispensable for Tooth Development[J]. Front Physiol,2021,12:660644. doi: 10.3389/fphys.2021.660644
[9]	Yang S,Choi H,Kim T H,et al. Cell dynamics in Hertwig’s epithelial root sheath are regulated by beta-catenin activity during tooth root development[J]. J Cell Physiol,2021,236(7):5387-5398. doi: 10.1002/jcp.30243
[10]	Nemoto E,Sakisaka Y,Tsuchiya M,et al. Wnt3a signaling induces murine dental follicle cells to differentiate into cementoblastic/osteoblastic cells via an osterix-dependent pathway[J]. J Periodontal Res,2016,51(2):164-174. doi: 10.1111/jre.12294
[11]	Huang H,Wang J,Zhang Y,et al. Bone resorption deficiency affects tooth root development in RANKL mutant mice due to attenuated IGF-1 signaling in radicular odontoblasts[J]. Bone,2018,114:161-171. doi: 10.1016/j.bone.2017.12.026
[12]	Nakatomi M,Morita I,Eto K,et al. Sonic hedgehog signaling is important in tooth root development[J]. J Dent Res,2006,85(5):427-431. doi: 10.1177/154405910608500506
[13]	Yokohama-tamaki T,Ohshima H,Fujiwara N,et al. Cessation of Fgf10 signaling,resulting in a defective dental epithelial stem cell compartment,leads to the transition from crown to root formation[J]. Development,2006,133(7):1359-1366. doi: 10.1242/dev.02307
[14]	Wang J,Feng J Q. Signaling Pathways Critical for Tooth Root Formation[J]. J Dent Res,2017,96(11):1221-1228. doi: 10.1177/0022034517717478
[15]	Li J,Parada C,Chai Y. Cellular and molecular mechanisms of tooth root development[J]. Development,2017,144(3):374-384. doi: 10.1242/dev.137216
[16]	Huang X,Xu X,Bringas P J,et al. Smad4-Shh-Nfic signaling cascade-mediated epithelial-mesenchymal interaction is crucial in regulating tooth root development[J]. J Bone Miner Res,2010,25(5):1167-1178.
[17]	Tian Q,Wang Y Y,Li Q,et al. Expressions of cytokeratin 14 and proliferating cell nuclear antigen in the Hertwig’s epithelial root sheath of a Vps4b knockout mouse[J]. Hua Xi Kou Qiang Yi Xue Za Zhi,2021,39(3):274-278.
[18]	Date Y,Kondo H,Yamashita A,et al. Combined in silico analysis identified a putative tooth root formation-related gene,Chd3,which regulates DNA synthesis in HERS01a cells[J]. Odontology,2020,108(3):386-395. doi: 10.1007/s10266-020-00489-w
[19]	Date Y,Yokoyama Y,Kondo H,et al. Restricted expression of chromatin remodeling associated factor Chd3 during tooth root development[J]. J Periodontal Res,2012,47(2):180-187. doi: 10.1111/j.1600-0765.2011.01419.x
[20]	Xu J,Kawashima N,Fujiwara N,et al. Promotional effects of vasoactive intestinal peptide on the development of rodent Hertwig’s epithelial root sheath[J]. Congenit Anom (Kyoto),2012,52(3):162-167. doi: 10.1111/j.1741-4520.2012.00371.x
[21]	Ida-yonemochi H,Satokata I,Ohshima H,et al. Morphogenetic roles of perlecan in the tooth enamel organ:an analysis of overexpression using transgenic mice[J]. Matrix Biol,2011,30(7-8):379-388. doi: 10.1016/j.matbio.2011.08.001
[22]	Sakuraba H,Fujiwara N,Sasaki-oikawa A,et al. Hepatocyte growth factor stimulates root growth during the development of mouse molar teeth[J]. J Periodontal Res,2012,47(1):81-88. doi: 10.1111/j.1600-0765.2011.01407.x
[23]	Fujiwara N,Lee J W,Kumakami-sakano M,et al. Harmine promotes molar root development via SMAD1/5/8 phosphorylation[J]. Biochem Biophys Res Commun,2018,497(3):924-929. doi: 10.1016/j.bbrc.2017.12.062
[24]	Fujiwara N,Akimoto T,Otsu K,et al. Reduction of Egf signaling decides transition from crown to root in the development of mouse molars[J]. J Exp Zool B Mol Dev Evol,2009,312B(5):486-494. doi: 10.1002/jez.b.21268
[25]	Fujiwara N,Tabata M J,Endoh M,et al. Insulin-like growth factor-I stimulates cell proliferation in the outer layer of Hertwig’s epithelial root sheath and elongation of the tooth root in mouse molars in vitro[J]. Cell Tissue Res,2005,320(1):69-75. doi: 10.1007/s00441-004-1065-5
[26]	Kawakami T,Nakamura Y,Karibe H. Cyclophosphamide inhibits root development of molar teeth in growing mice[J]. Odontology,2015,103(2):143-151. doi: 10.1007/s10266-014-0158-1
[27]	Hirose N,Shimazu A,Watanabe M,et al. Ameloblastin in Hertwig’s epithelial root sheath regulates tooth root formation and development[J]. PLoS One,2013,8(1):e54449. doi: 10.1371/journal.pone.0054449
[28]	Ide Y,Nakahara T,Nasu M,et al. Cell dynamics in Hertwig’s epithelial root sheath and surrounding mesenchyme in mice irradiated to the head[J]. Oral Dis,2015,21(2):232-239. doi: 10.1111/odi.12253
[29]	Nakasone N,Yoshie H. Occlusion regulates tooth-root elongation during root development in rat molars[J]. Eur J Oral Sci,2011,119(6):418-426. doi: 10.1111/j.1600-0722.2011.00856.x
[30]	Hodgson B D,Pyszka B,Henry M M,et al. Effect of photobiomodulation on vinblastine-poisoned murine HERS cells[J]. Photomed Laser Surg,2011,29(4):233-237. doi: 10.1089/pho.2010.2794
[31]	Jiang X,Liu H. An uncommon type of segmental root development after revitalization[J]. Int Endod J,2020,53(12):1728-1741. doi: 10.1111/iej.13387
[32]	Jung I Y,Kim E S,Lee C Y,et al. Continued development of the root separated from the main root[J]. J Endod,2011,37(5):711-714. doi: 10.1016/j.joen.2011.01.015
[33]	Andreasen J O,Kristerson L,Andreasen F M. Damage of the Hertwig’s epithelial root sheath:effect upon root growth after autotransplantation of teeth in monkeys[J]. Endod Dent Traumatol,1988,4(4):145-151. doi: 10.1111/j.1600-9657.1988.tb00313.x
[34]	Andreasen J O,Paulsen H U,Yu Z,et al. A long-term study of 370 autotransplanted premolars. Part IV. Root development subsequent to transplantation[J]. Eur J Orthod,1990,12(1):38-50. doi: 10.1093/ejo/12.1.38
[35]	He M,Wang P,Li B,et al. Rodent incisor and molar dental follicles show distinct characteristics in tooth eruption[J]. Arch Oral Biol,2021,126:105117. doi: 10.1016/j.archoralbio.2021.105117
[36]	Yang Y,Ge Y,Chen G,et al. Hertwig's epithelial root sheath cells regulate osteogenic differentiation of dental follicle cells through the Wnt pathway[J]. Bone,2014,63:158-165. doi: 10.1016/j.bone.2014.03.006
[37]	Guo Y,Guo W,Chen J,et al. Are Hertwig's epithelial root sheath cells necessary for periodontal formation by dental follicle cells?[J]. Arch Oral Biol,2018,94:1-9. doi: 10.1016/j.archoralbio.2018.06.014
[38]	Guo Y,Guo W,Chen J,et al. Comparative study on differentiation of cervical-loop cells and Hertwig’s epithelial root sheath cells under the induction of dental follicle cells in rat[J]. Sci Rep,2018,8(1):6546. doi: 10.1038/s41598-018-24973-0
[39]	Jung H S,Lee D S,Lee J H,et al. Directing the differentiation of human dental follicle cells into cementoblasts and/or osteoblasts by a combination of HERS and pulp cells[J]. J Mol Histol,2011,42(3):227-235. doi: 10.1007/s10735-011-9327-5
[40]	Bai Y,Bai Y,Matsuzaka K,et al. Cementum- and periodontal ligament-like tissue formation by dental follicle cell sheets co-cultured with Hertwig’s epithelial root sheath cells[J]. Bone,2011,48(6):1417-1426. doi: 10.1016/j.bone.2011.02.016
[41]	Duan Y,Li X,Zhang S,et al. Therapeutic potential of HERS spheroids in tooth regeneration[J]. Theranostics,2020,10(16):7409-7421. doi: 10.7150/thno.44782
[42]	Thomas H F,Kollar E J. Differentiation of odontoblasts in grafted recombinants of murine epithelial root sheath and dental mesenchyme[J]. Arch Oral Biol,1989,34(1):27-35. doi: 10.1016/0003-9969(89)90043-5
[43]	Oh J E,Yi J K. Isolation and characterization of dental follicle-derived Hertwig’s epithelial root sheath cells[J]. Clin Oral Investig,2021,25(4):1787-1796. doi: 10.1007/s00784-020-03481-4
[44]	Chen J,Chen G,Yan Z,et al. TGF-beta1 and FGF2 stimulate the epithelial-mesenchymal transition of HERS cells through a MEK-dependent mechanism[J]. J Cell Physiol,2014,229(11):1647-1659. doi: 10.1002/jcp.24610
[45]	Li Y,Liu A,Zhang L,et al. Epithelial Cell Rests of Malassez Provide a Favorable Microenvironment for Ameliorating the Impaired Osteogenic Potential of Human Periodontal Ligament Stem Cells[J]. Front Physiol,2021,12:735234. doi: 10.3389/fphys.2021.735234
[46]	Bosshardt D D,Stadlinger B,Terheyden H. Cell-to-cell communication-periodontal regeneration[J]. Clin Oral Implants Res,2015,26(3):229-239. doi: 10.1111/clr.12543
[47]	Huang X,Bringas P J,Slavkin H C,et al. Fate of HERS during tooth root development[J]. Dev Biol,2009,334(1):22-30. doi: 10.1016/j.ydbio.2009.06.034
[48]	Xiong J,Gronthos S,Bartold P M. Role of the epithelial cell rests of Malassez in the development,maintenance and regeneration of periodontal ligament tissues[J]. Periodontol,2013,63(1):217-233.
[49]	Kim G H, Yang J, Jeon D H, et al. Differentiation and Establishment of Dental Epithelial-Like Stem Cells Derived from Human ESCs and iPSCs[J]. Int J Mol Sci, 2020, 21（12）: 220-226.
[50]	Li X,Zhang S,Zhang Z,et al. Development of immortalized Hertwig’s epithelial root sheath cell lines for cementum and dentin regeneration[J]. Stem Cell Res Ther,2019,10(1):3. doi: 10.1186/s13287-018-1106-8
[51]	Zhang S,Li X,Wang S,et al. Immortalized Hertwig’s epithelial root sheath cell line works as model for epithelial-mesenchymal interaction during tooth root formation[J]. J Cell Physiol,2020,235(3):2698-2709. doi: 10.1002/jcp.29174
[52]	Tsunematsu T,Fujiwara N,Yoshida M,et al. Human odontogenic epithelial cells derived from epithelial rests of Malassez possess stem cell properties[J]. Lab Invest,2016,96(10):1063-1075. doi: 10.1038/labinvest.2016.85
[53]	Nam H,Kim J,Park J,et al. Expression profile of the stem cell markers in human Hertwig’s epithelial root sheath/Epithelial rests of Malassez cells[J]. Mol Cells,2011,31(4):355-360. doi: 10.1007/s10059-011-0045-3
[54]	Nam H,Kim J H,Kim J W,et al. Establishment of Hertwig’s epithelial root sheath/epithelial rests of Malassez cell line from human periodontium[J]. Mol Cells,2014,37(7):562-567. doi: 10.14348/molcells.2014.0161
[55]	Akimoto T,Fujiwara N,Kagiya T,et al. Establishment of Hertwig’s epithelial root sheath cell line from cells involved in epithelial-mesenchymal transition[J]. Biochem Biophys Res Commun,2011,404(1):308-312. doi: 10.1016/j.bbrc.2010.11.112
[56]	Zhang S,Yang Y,Jia S,et al. Exosome-like vesicles derived from Hertwig’s epithelial root sheath cells promote the regeneration of dentin-pulp tissue[J]. Theranostics,2020,10(13):5914-5931. doi: 10.7150/thno.43156
[57]	Diekwisch T G. The developmental biology of cementum[J]. Int J Dev Biol,2001,45(5-6):695-706.
[58]	Yamamoto T,Yamada T,Yamamoto T,et al. Hertwig’s Epithelial Root Sheath Fate during Initial Cellular Cementogenesis in Rat Molars[J]. Acta Histochem Cytochem,2015,48(3):95-101. doi: 10.1267/ahc.15006
[59]	Yamamoto T,Yamamoto T,Yamada T,et al. Hertwig’s epithelial root sheath cell behavior during initial acellular cementogenesis in rat molars[J]. Histochem Cell Biol,2014,142(5):489-496. doi: 10.1007/s00418-014-1230-1
[60]	Yamamoto T,Takahashi S. Hertwig’s epithelial root sheath cells do not transform into cementoblasts in rat molar cementogenesis[J]. Ann Anat,2009,191(6):547-555. doi: 10.1016/j.aanat.2009.07.004
[61]	Bosshardt D D,Nanci A. Immunodetection of enamel- and cementum-related (bone) proteins at the enamel-free area and cervical portion of the tooth in rat molars[J]. J Bone Miner Res,1997,12(3):367-379. doi: 10.1359/jbmr.1997.12.3.367
[62]	Bosshardt D D. Are cementoblasts a subpopulation of osteoblasts or a unique phenotype?[J]. J Dent Res,2005,84(5):390-406. doi: 10.1177/154405910508400501
[63]	Bosshardt D D,Nanci A. Immunolocalization of epithelial and mesenchymal matrix constituents in association with inner enamel epithelial cells[J]. J Histochem Cytochem,1998,46(2):135-142. doi: 10.1177/002215549804600201
[64]	Bosshardt D D,Nanci A. Hertwig’s epithelial root sheath,enamel matrix proteins,and initiation of cementogenesis in porcine teeth[J]. J Clin Periodontol,2004,31(3):184-192. doi: 10.1111/j.0303-6979.2004.00473.x
[65]	Hammarstrom L,Alatli I,Fong C D. Origins of cementum[J]. Oral Dis,1996,2(1):63-69.
[66]	Alatli I,Lundmark C,Hammarstrom L. The localization of epithelial root sheath cells during cementum formation in rat molars[J]. J Periodontal Res,1996,31(6):433-440. doi: 10.1111/j.1600-0765.1996.tb00512.x
[67]	Zeichner-david M,Oishi K,Su Z,et al. Role of Hertwig’s epithelial root sheath cells in tooth root development[J]. Dev Dyn,2003,228(4):651-663. doi: 10.1002/dvdy.10404
[68]	Sonoyama W,Seo B M,Yamaza T,et al. Human Hertwig’s epithelial root sheath cells play crucial roles in cementum formation[J]. J Dent Res,2007,86(7):594-599. doi: 10.1177/154405910708600703
[69]	Itaya S,Oka K,Ogata K,et al. Hertwig’s epithelial root sheath cells contribute to formation of periodontal ligament through epithelial-mesenchymal transition by TGF-beta[J]. Biomed Res,2017,38(1):61-69. doi: 10.2220/biomedres.38.61

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赫特维希上皮根鞘细胞的研究进展

doi: 10.12259/j.issn.2095-610X.S20220628

作者简介:
毕菲（1995～），女，云南昆明人，在读硕士研究生，主要从事儿童口腔早期矫治临床工作及牙源性干细胞相关基础研究工作

通讯作者:
郭维华，E-mail：guoweihua943019@163.com

计量

Advances in Research on Hertwig’s Epithelial Root Sheath Cells

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目录

[1]	李鹏, 邹仁超, 代龙翱, 傅嵩洲, 魏东, 唐波. HIF-1α通过调控上皮-间充质转化对胆囊癌侵袭转移的影响, 昆明医科大学学报.
[2]	杨镕羽, 宋飞, 黄浩, 段开文, 向盈盈. 骨髓间充质干细胞在口腔医学中的应用, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20230323
[3]	马丽娅, 饶南荃, 杨禾丰. 间充质干细胞外泌体在口腔组织再生中的研究进展, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20210527
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[10]	康强. E-cadherin和Vimentin在肝内胆管癌的表达及临床意义, 昆明医科大学学报.
[11]	易敏. 兔骨髓间充质干细胞的培养及初步鉴定, 昆明医科大学学报.
[12]	邰文琳. 骨髓间充质干细胞抗肺泡上皮细胞凋亡作用研究, 昆明医科大学学报.
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[17]	邰文琳. 小鼠骨髓间充质干细胞体外扩增的实验研究, 昆明医科大学学报.
[18]	黄颖. 人脐带间充质干细胞对再生障碍性贫血患者T细胞相关因子调节的体外研究, 昆明医科大学学报.
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[20]	杨锋. 两种培养方法在猪自体骨髓间充质干细胞培养的比较研究, 昆明医科大学学报.

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赫特维希上皮根鞘细胞的研究进展

doi: 10.12259/j.issn.2095-610X.S20220628

作者简介: 毕菲（1995～ ），女，云南昆明人，在读硕士研究生，主要从事儿童口腔早期矫治临床工作及牙源性干细胞相关基础研究工作

通讯作者: 郭维华，E-mail：guoweihua943019@163.com

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Advances in Research on Hertwig’s Epithelial Root Sheath Cells

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作者简介:
毕菲（1995～），女，云南昆明人，在读硕士研究生，主要从事儿童口腔早期矫治临床工作及牙源性干细胞相关基础研究工作

通讯作者:
郭维华，E-mail：guoweihua943019@163.com