Effect of VEGF-C on Radiation Sensitivity of Nasopharyngeal Carcinoma CNE-2 Cell Transplanted Mice
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摘要:
目的 探讨干扰VEGF-C表达可以增加鼻咽癌CNE-2细胞小鼠移植瘤辐射敏感性作用以及相关信号途径。 方法 鼻咽癌CNE-2小鼠移植瘤随机分为：瘤体组；siRNA-NC组；siRNA-VEGF-C组；照射组；siRNA-NC+照射组；siRNA-VEGF-C+照射组。观察肿瘤生长情况；采用免疫组化检测VEGF-C、ATM、P65蛋白的表达变化。 结果 干扰VEGF-C表达鼻咽癌CNE-2细胞小鼠移植瘤，在照射后明显肿瘤生长明显变慢。免疫组化检测结果显示：干扰VEGF-C小鼠移植瘤后，VEGF-C、ATM明显减少（P < 0.05），P65增加（P < 0.05）；联合照射后，VEGF-C、ATM明显减少（P < 0.05）。 结论 在鼻咽癌CNE-2细胞小鼠移植瘤中，干扰VEGF-C基因通过激活NF-kB信号通路影响下游ATM，进而增加放疗敏感性。 -
关键词:
- 鼻咽癌 /
- 血管内皮生长因子-C /
- 辐射敏感性 /
- 实验动物
Abstract:Objective To investigate the effect of interfering with the expression of VEGF-C on the radiosensitivity of the nasopharyngeal carcinoma CNE-2 cell transplanted tumor and related signaling pathways. Methods The mice with transplanted tumors of nasopharyngeal carcinoma CNE-2 were randomly divided into tumor body group, siRNA-NC group, siRNA-VEGF-C group; irradiation group; siRNA-NC+ irradiation group; siRNA-VEGF-C+irradiation group. Tumor growth was observed, and the protein expression of VEGF-C, ATM and P65 were detected by immunohistochemistry. Results Interference of VEGF-C expression in nasopharyngeal carcinoma CNE-2 cell transplanted tumors showed significantly slower tumor growth after irradiation. Immunohistochemical test results showed that after tumor transplantation of mice interfered with VEGF-C, VEGF-C and ATM were significantly decreased （P < 0.05）, while P65 was increased （P < 0.05）. After combined irradiation, VEGF-C and ATM were significantly decreased （P < 0.05）. Conclusion In mouse nasopharyngeal carcinoma CNE-2 cell transplantation tumors, interfering with VEGF-C gene affects downstream ATM by activating NF-kB signaling pathway, thereby increasing the sensitivity of radiotherapy. -
Key words:
- Nasopharyngeal carcinoma /
- VEGF-C /
- Radiation sensitivity /
- Experimental animals
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图 1 鼻咽癌细胞CNE-2中VEGF-C干扰序列筛选
A：干扰VEGF-C的CNE-2细胞，0、12、24和36 h后，CCK-8检测细胞活力检测；B：3组siRNA采用qRT-PCR测定的VEGF-C mRNA表达；C：Western blot检测VEGF-C蛋白的表达；D：VEGF-C蛋白的表达水平。与NC对照组比较，*P < 0.05，**P < 0.01。
Figure 1. The establishment of VEGF-C silenced NPC cell lines
图 2 动物实验检测干扰VEGF-C对肿瘤大小影响
n = 6。
Figure 2. Animal experiment to detect the influence of interfering VEGF-C on tumor size
图 3 免疫组化检测VEGF-C表达（×400）
A：瘤体组；B：阴性对照组；C：si-VEGF-C组；D：8 Gy照射组；E：代表阴性对照组+照射组；F：代表si-VEGF-C+照射组；G：免疫组化平均光密度统计分析（横线两端所示意2组间比较）。*P < 0.05。
Figure 3. Immunohistochemical detection of VEGF-C expression （×400）
图 4 免疫组化检测p65表达（×400）
A：瘤体组；B：阴性对照组；C：si-VEGF-C组；D：8 Gy照射组；E：阴性对照组+照射组；F：si-VEGF-C+照射组；G：免疫组化平均光密度统计分析。与瘤体组相比，*P < 0.05。
Figure 4. Immunohistochemical detection of p65 expression（×400）
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