Chemical Compounds Isolated from Pleione maculata and Their Bioactive Activity
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摘要:
目的 研究秋花独蒜兰化学成分，发现其活性成分。 方法 秋花独蒜兰经95%乙醇提取、硅胶柱层析、半制备HPLC和Sephadex LH-20柱层析进行分离纯化，波谱分析（核磁共振氢谱、碳谱、和质谱）确定结构；应用MTT法，对部分化合物进行体外抗肿瘤活性筛选。 结果 从秋花独蒜兰分离鉴定10个化合物，分别为：1，7-dihydroxy-2，5-dimethoxyphenanthrene （ 1 ），2，7-dihydroxy-1，5-dime-thoxyphenanthrene （ 2 ），confusarin （ 3 ），4，7-dihydroxy-2-dimethoxy-9，10-dihydrophe-nanthrene （ 4 ），pleionesin B （ 5 ），pleionesin C （ 6 ），flavanthrin （ 7 ），2，2′-dihydroxy-5，5′，7，7′-tetramethoxy-9，9′，10，10′-tetrahydro-3，3′-biphenanthrene （ 8 ），6，6′，7，7′-tetrahy--droxy-2，2′，4，4′-tetramethoxy -8，8′-biphenanthrene （ 9 ），1，3′，5′，7-tetrahydroxy-4，7′-dimethoxy-9，9′，10，10′-tetrahydro-2，2′-biphenanthrene （1 0 ）；对化合物3，7～10 A549、MCF-7/S和SKOV-3进行肿瘤细胞株抑制活性测试，发现化合物10对3种细胞株活性较好。 结论 所有化合物为首次从本植物中分离得到，研究结果表明化合物10对3种肿瘤细胞株具有很好的抑制活性，其IC50分别为2.45，6.83，4.23 μM。 Abstract:Objective To study the chemical compounds from the medicinal plants of Pleione maculata and find its bioactive compounds. Methods The compounds were extracted by alcohol （95%） and isolated by column chromatography on silica gel and Sephadex LH-20. Their structures were identified by spectroscopic analysis （1H NMR, 13CNMR and EIMS）. The antitumor activity of the compounds was studied by MTT assay in vitro. Results Ten compounds were obtained and identified as 1, 7-dihydroxy-2, 5 -dimethoxyphenanthrene （ 1 ）, 2, 7-dihydroxy-1, 5-dimethoxyphenanthrene （ 2 ）, confusarin （ 3 ）, 4, 7-dihydroxy-2-dimethoxy-9, 10-dihydrophenanthrene （ 4 ）, pleionesin B （ 5 ）, pleionesin C （ 6 ）, flavanthrin （ 7 ）, 2, 2′-dihydroxy-5, 5′, 7, 7′-tetramethoxy-9, 9′, 10, 10′-tetrahydro-3, 3′-biphenanthrene （ 8 ）, 6, 6′, 7, 7′-tetrahy- -droxy-2, 2′, 4, 4′-tetramethoxy -8, 8′-biphenanthrene （ 9 ）, 1, 3′, 5′, 7-tetrahydroxy-4, 7′ -dimethoxy-9, 9′, 10, 10′-tetrahydro-2, 2′- biphenanthrene （1 0 ）; Compounds 3, 7～10 were tested for the inhibitory activity of human lung cancer cell lines （A549）, human breast cancer drug-sensitive cell lines （MCF-7/S）, and human ovarian cancer cell lines （SKOV-3）. The results of anti-tumor activity test of compounds showed that 10 showed good inhibitory activity on three cell lines. Conclusion All compounds were isolated from this plant for the first time. Compound 10 has good inhibitory activity against three tumor cell lines, and with IC50 of 2.45, 6.83, 4.23 μM, respectively. -
Key words:
- Pleione maculata /
- Chemical constituents /
- Bioactive activity
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表 1 化合物3，7～10对3种细胞株的细胞毒活性IC50 （μM）
Table 1. Cytotoxic activities of compounds 3，7～10 on three cell lines IC50 （μM）
化合物 A549 MCF-7/S SKOV-3 3 163.6 33.42 98.49 7 106.8 112.2 76.08 8 91.2 41.5 22.4 9 38.91 102.2 56.92 10 2.45 6.83 4.23 DDP 22.02 10.02 14.15 
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