留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

大鼠伏核甘丙肽镇痛作用及其机制

彭煜航 李焱娟 曲婧红 唐代欢 张宏伟 吴睿 徐世莲

彭煜航, 李焱娟, 曲婧红, 唐代欢, 张宏伟, 吴睿, 徐世莲. 大鼠伏核甘丙肽镇痛作用及其机制[J]. 昆明医科大学学报, 2023, 44(9): 1-6. doi: 10.12259/j.issn.2095-610X.S20230925
引用本文: 彭煜航, 李焱娟, 曲婧红, 唐代欢, 张宏伟, 吴睿, 徐世莲. 大鼠伏核甘丙肽镇痛作用及其机制[J]. 昆明医科大学学报, 2023, 44(9): 1-6. doi: 10.12259/j.issn.2095-610X.S20230925
Yuhang PENG, Yanjuan LI, Jinghong QU, Daihuan TANG, Hongwei ZHANG, Rui WU, Shilian XU. The Antinociception of Galanin on Nucleus Accumbens and Its Mechanism in Rats[J]. Journal of Kunming Medical University, 2023, 44(9): 1-6. doi: 10.12259/j.issn.2095-610X.S20230925
Citation: Yuhang PENG, Yanjuan LI, Jinghong QU, Daihuan TANG, Hongwei ZHANG, Rui WU, Shilian XU. The Antinociception of Galanin on Nucleus Accumbens and Its Mechanism in Rats[J]. Journal of Kunming Medical University, 2023, 44(9): 1-6. doi: 10.12259/j.issn.2095-610X.S20230925

大鼠伏核甘丙肽镇痛作用及其机制

doi: 10.12259/j.issn.2095-610X.S20230925
基金项目: 国家自然科学基金资助项目(82160228 );云南省大学生创新性实验基金资助项目(2022JXD281)
详细信息
    作者简介:

    彭煜航(1999~),男,福建仙游人,在读硕士研究生,主要从事生理学科学研究工作

    通讯作者:

    徐世莲,E-mail:xushilian@kmmu.edu.cn

  • 中图分类号: R338.3

The Antinociception of Galanin on Nucleus Accumbens and Its Mechanism in Rats

  • 摘要:   目的  研究伏核甘丙肽对正常大鼠、炎症痛大鼠和神经痛大鼠的镇痛作用,并初步探索其机制。  方法  炎症痛动物模型采用鹿角菜碱足底皮下注射的方法制备,神经痛动物模型采用左侧坐骨神经结扎的方法制备。测量大鼠的后爪缩爪潜伏期(hind-paw withdraw latency,HWL)和后爪缩爪阈值(hind-paw withdraw threshold,HWT)来反应痛阈;使用蛋白免疫印迹法检测大鼠伏核甘丙肽受体(galanin receptors,GalRs)的表达。  结果  (1)伏核注射甘丙肽引起正常大鼠(P < 0.05;P < 0.01)、炎症痛大鼠(P < 0.001;P < 0.001)和神经痛大鼠(P < 0.001;P < 0.001)的HWL延长,且正常大鼠(P < 0.001;P < 0.01)、炎症痛大鼠(P < 0.001;P < 0.001)和神经痛大鼠(P < 0.001;P < 0.001)的HWT增加;(2)比较甘丙肽对3种大鼠的镇痛作用,甘丙肽对炎症痛大鼠(HWL:P < 0.05,P < 0.05;HWT:P < 0.01,P < 0.05)和神经痛大鼠(HWL:P < 0.05,P < 0.05;HWT:P < 0.001,P < 0.001)的镇痛作用比对正常大鼠的镇痛作用强;但比较甘丙肽对炎症痛大鼠和神经痛大鼠的镇痛作用,差异无统计学意义(P > 0.05);(3)足底皮下注射鹿角菜碱后3 h(P < 0.001)和坐骨神经结扎后14 d(P < 0.001)引起大鼠伏核神经细胞GalR1的表达上调,且注射鹿角菜碱后3 h(P < 0.05)和坐骨神经结扎后14 d(P < 0.05)大鼠伏核神经细胞GalR2的表达也上调。  结论  伏核甘丙肽对正常大鼠、炎症痛大鼠和神经痛大鼠都具有镇痛作用,甘丙肽通过和其受体结合对大鼠具有镇痛作用。
  • 图  1  伏核注射位点鉴定图

    NAc:伏核。

    Figure  1.  Illustration of the location of the injection needle tips

    图  2  伏核注射甘丙肽对正常大鼠、炎症痛大鼠和神经痛大鼠 HWL 和 HWT 的影响

    A:左 HWL、B:右 HWL、C:左 HWT、D:右 HWT。 与生理盐水对照组比较, *P < 0.05、 **P < 0.01、***P < 0.001。

    Figure  2.  Effects of intra-NAc injection of galanin on HWL and HWT in normal, inflammatory and CCI rats

    图  3  甘丙肽对正常大鼠、炎症痛大鼠和神经痛大鼠的镇痛作用比较

    与正常组比较,*P < 0.05、**P < 0.01、***P < 0.001。

    Figure  3.  Comparison of analgesic effects of galanin on native, inflammatory and CCI rats

    图  4  正常大鼠、炎症痛大鼠和神经痛大鼠 GalR1 和 GalR2 的表达

    A:Western blot 检测GalR1、GalR2和GAPDH的表达条带;B:GalR1或GalR2/GAPDH的比值图与正常组比较,*P < 0.05、***P < 0.001。

    Figure  4.  Expression of GalR1 and GalR2 in native, inflammatory and CCI rats

  • [1] Tatemoto K,Rökaeus A,Jörnvall H,et al. Galanin-a novel biologically active peptide from porcine intestine[J]. FEBS Lett,1983,164(1):124-128. doi: 10.1016/0014-5793(83)80033-7
    [2] Taylor T S,Konda P,John S S,et al. Galanin suppresses visceral afferent responses to noxious mechanical and inflammatory stimuli[J]. Physiological Reports,2020,8(2):e14326.
    [3] Yu M,Fang P H,Wang H,et al. Beneficial effects of galanin system on diabetic peripheral neuropathic pain and its complications[J]. Peptides,2020,134(12):170404.
    [4] Liu F, Yajima T, Wang M, et al. Effects of trigeminal nerve injury on the expression of galanin and its receptors in the rat trigeminal ganglion [J]. Neuropeptides, 2020, 84(期号!): 102098.

    Liu F,Yajima T,Wang M,et al. Effects of trigeminal nerve injury on the expression of galanin and its receptors in the rat trigeminal ganglion[J]. Neuropeptides,2020,84:102098.
    [5] Amorim D,David-Pereira A,Marques P,et al. A role of supraspinal galanin in behavioural hyperalgesia in the rat[J]. Plos one,2014,9(11):e113077. doi: 10.1371/journal.pone.0113077
    [6] Dias E V,Sartori C R,Mariao P R,et al. Nucleus accumbens dopaminergic neurotransmission switches its modulatory action in chronification of inflammatory hyperalgesia[J]. Eur J Neurosci,2015,42(7):2380-2389. doi: 10.1111/ejn.13015
    [7] Duan H,Zhang Y,Zhang X M,et al. Antinociceptive roles of galanin receptor 1 in nucleus accumbens of rats in a model of neuropathic pain[J]. J Neurosci Res,2015,93(10):1542-1551. doi: 10.1002/jnr.23611
    [8] Mitsukawa K,Lu X,Bartfai T. Galanin,galanin receptors and drug targets[J]. Cell Mol Life Sci,2008,65(12):1796-1805. doi: 10.1007/s00018-008-8153-8
    [9] Gear R W,Aley K O,Levine J D. Pain—induced analgesia mediated by mesolimbic reward circuits[J]. J Neurosci,1999,19(16):7175-7181. doi: 10.1523/JNEUROSCI.19-16-07175.1999
    [10] Watanabe M,Sugiura Y,Sugiyama E,et al. Extracellular N- acetylaspartylglutamate released in the nucleus accumbens modulates the pain sensation: Analysis using a microdialysis/mass spectrometry integrated system[J]. Mol Pain,2018,14:1744806918754934.
    [11] Zhang Y, Gao Y, Li CY, et al. Galanin plays a role in antinociception via binding to galanin receptors in the nucleus accumbens of rats with neuropathic pain [J]. Neurosci Lett, 2019, 706(期号?): 93-98.

    Zhang Y,Gao Y,Li C Y,et al. Galanin plays a role in antinociception via binding to galanin receptors in the nucleus accumbens of rats with neuropathic pain[J]. Neurosci Lett,2019,706:93-98.
    [12] Zhang, ML, Fu, FH, Yu, LC. Antinociception induced by galanin in anterior cingulate cortex in rats with acute inflammation [J]. Neurosci. Lett. 2017, 638(期号?): 156-161.

    Zhang M L,Fu F H,Yu L C. Antinociception induced by galanin in anterior cingulate cortex in rats with acute inflammation[J]. Neurosci Lett,2017,638:156-161.
    [13] Zhang M L,Wang H B,Fu F H,et al. Involvement of galanin and galanin receptor 2 in nociceptive modulation in anterior cingulate cortex of normal rats and rats with mononeuropathy[J]. Sci Rep,2017,7(1):45930. doi: 10.1038/srep45930
    [14] Li S Y,Huo M L,Wu X Y,et al. Involvement of galanin and galanin receptor 1 in nociceptive modulation in the central nucleus of amygdala in normal and neuropathic rats[J]. Sci Rep,2017,7(1):15317. doi: 10.1038/s41598-017-13944-6
    [15] Li L X, Wang X B, Yu L C. Involvement of opioid receptors in the CGRP-induced antinociception in the nucleus accumbens of rats[J]. Brain Res,2010,1353:53-59.
    [16] Hobson S A,Bacon A,Elliot-Hunt C R,et al. Galanin acts as a trophic factor to the central and peripheral nervous systems[J]. Cell Mol Life Sci,2008,65(12):1806-1812.
    [17] Barreda-Gómez G,Giralt M T,Rodríguez-Puertas R. G protein-coupled galanin receptor distribution in the rat central nervous system[J]. Neuropeptides,2005,39(3):153-156.
    [18] Lang R,Gundlach A L,Holmes F E,et al. Physiology,signaling,and pharmacology of galanin peptides and receptors: Three decades of emerging diversity[J]. Pharmacol Rev,2015,67(1):118-175. doi: 10.1124/pr.112.006536
    [19] Chen S H,Lue J H,Hsiao Y J,et al. Elevated galanin receptor type 2 primarily contributes to mechanical hypersensitivity after median nerve injury[J]. PLoS ONE,2018,13(6):e0199512.
    [20] Xu X F,Yang X D,Zhang P,et al. Effects of exogenous galanin on neuropathic pain state and change of galanin and its receptors in DRG and SDH after sciatic nerve-pinch injury in rat[J]. Plos One,2012,7(5):e37621. doi: 10.1371/journal.pone.0037621
    [21] Dong Yan,Li Chongyang,Zhang Xiaomin,et al. The activation of galanin receptor 2 plays an antinociceptive effect in nucleus accumbens of rats with neuropathic pain[J]. J Physiol Sci,2021,71(1):6. doi: 10.1186/s12576-021-00790-5
    [22] Mengnan Li, Xiaomin Zhang, Chongyang Li, et al. Galanin receptor 2 is involved in galanin-induced analgesic effect by activating PKC and CaMKII in the nucleus accumbens of inflammatory pain rats [J]. Front Neurosci, 2021, 14(期号?): 593331.

    Li Mengnan,Zhang Xiaomin,Li Chongyang,et al. Galanin receptor 2 is involved in galanin-induced analgesic effect by activating PKC and CaMKII in the nucleus accumbens of inflammatory pain rats[J]. Front Neurosci,2021,14:593331.
  • [1] 毛健宇, 陈建军, 阎静楠, 王泉, 詹力, 李石乔, 保文莉.  超声引导下肋间神经脉冲射频治疗胸椎高节段带状疱疹后神经痛的临床疗效, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20240514
    [2] 刘亚南, 杨双, 彭煜航, 陈静, 徐世莲.  针刀疗法对神经痛大鼠的镇痛作用研究, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20231009
    [3] 杨振东, 兰云艳, 郑宇, 冯煜然, 朱梅.  超声刺激小鼠伏隔核后c-Fos蛋白及结构可塑性改变的实验, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20210645
    [4] 兰云艳, 冯煜然, 郑宇, 杨振东, 赵诣, 朱梅.  低强度聚焦超声刺激小鼠伏隔核的有效性, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20210703
    [5] 郭欣, 赖薇, 李艳华, 陈韵如, 叶傲然, 唐顺松, 罗静.  磷脂酶Cε1在1型糖尿病大鼠病理性神经痛中的作用初探, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20210206
    [6] 刘亚南, 杨双, 董玮, 李崇阳, 徐世莲.  伏核神经细胞GalR2介导针刀干预神经病理性痛大鼠的镇痛作用, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20210914
    [7] 李梦楠, 徐焕焕, 刘亚南, 杨双, 李崇阳, 徐世莲.  伏核神经元ERK1/2在GalR1激活对神经痛大鼠镇痛作用中的机制, 昆明医科大学学报.
    [8] 张立, 董介轩, 殷志荣, 石小东, 李皎, 许燕飞, 刘维统, 董玮.  针刀松解对坐骨神经痛大鼠中枢镇痛机制, 昆明医科大学学报.
    [9] 董燕, 李梦楠, 刘亚南, 徐世莲.  甘丙肽受体2在病理性疼痛中的研究进展, 昆明医科大学学报.
    [10] 张沛, 邢群智, 李毓, 韩学昌, 董旭, 常俊晓.  电针镇痛治疗带状疱疹后神经痛的临床疗效, 昆明医科大学学报.
    [11] 张金培, 杨屹, 靳艳.  麻芎舒痛方治疗偏头痛的临床效果及对血清5-HT、NF-κB和炎症因子水平的影响, 昆明医科大学学报.
    [12] 张细丽, 孟汶, 李倩烨, 王国梁, 李彦林, 张小梅.  神经阻滞预防带状疱疹后神经痛及其高危因素分析, 昆明医科大学学报.
    [13] 魏辉明, 麻伟青, 董发团, 李文锋, 普俊杰, 贺焱峰.  神经病理性疼痛老年大鼠海马突触长时程抑制的变化, 昆明医科大学学报.
    [14] 庞佳宁, 刘劲松, 李溪, 王莉竹, 张锡华.  TKA术后连续股神经阻滞早期镇痛疗效, 昆明医科大学学报.
    [15] 王慧明.  地佐辛腹腔内注射减轻神经病理性疼痛大鼠的痛行为研究, 昆明医科大学学报.
    [16] 黄晓斌.  伽玛刀治疗吗啡依赖猴双侧伏隔核后多巴胺D3受体变化的研究, 昆明医科大学学报.
    [17] 张小超.  苦参胶囊抗炎镇痛作用研究, 昆明医科大学学报.
    [18] 徐阳.  伽玛刀治疗原发性三叉神经痛46例分析, 昆明医科大学学报.
    [19] 马加庆.  利胆止痛胶囊对小鼠抗抑菌炎、镇痛和利胆作用研究, 昆明医科大学学报.
    [20] 小剂量氯胺酮治疗慢性顽固性灼性神经痛1例报道, 昆明医科大学学报.
  • 加载中
图(4)
计量
  • 文章访问数:  1544
  • HTML全文浏览量:  1072
  • PDF下载量:  14
  • 被引次数: 0
出版历程
  • 收稿日期:  2023-04-01
  • 网络出版日期:  2023-09-23
  • 刊出日期:  2023-09-30

目录

    /

    返回文章
    返回