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大鼠伏核甘丙肽镇痛作用及其机制

彭煜航 李焱娟 曲婧红 唐代欢 张宏伟 吴睿 徐世莲

彭煜航, 李焱娟, 曲婧红, 唐代欢, 张宏伟, 吴睿, 徐世莲. 大鼠伏核甘丙肽镇痛作用及其机制[J]. 昆明医科大学学报, 2023, 44(9): 1-6. doi: 10.12259/j.issn.2095-610X.S20230925
引用本文: 彭煜航, 李焱娟, 曲婧红, 唐代欢, 张宏伟, 吴睿, 徐世莲. 大鼠伏核甘丙肽镇痛作用及其机制[J]. 昆明医科大学学报, 2023, 44(9): 1-6. doi: 10.12259/j.issn.2095-610X.S20230925
Yuhang PENG, Yanjuan LI, Jinghong QU, Daihuan TANG, Hongwei ZHANG, Rui WU, Shilian XU. The Antinociception of Galanin on Nucleus Accumbens and Its Mechanism in Rats[J]. Journal of Kunming Medical University, 2023, 44(9): 1-6. doi: 10.12259/j.issn.2095-610X.S20230925
Citation: Yuhang PENG, Yanjuan LI, Jinghong QU, Daihuan TANG, Hongwei ZHANG, Rui WU, Shilian XU. The Antinociception of Galanin on Nucleus Accumbens and Its Mechanism in Rats[J]. Journal of Kunming Medical University, 2023, 44(9): 1-6. doi: 10.12259/j.issn.2095-610X.S20230925

大鼠伏核甘丙肽镇痛作用及其机制

doi: 10.12259/j.issn.2095-610X.S20230925
基金项目: 国家自然科学基金资助项目(82160228 );云南省大学生创新性实验基金资助项目(2022JXD281)
详细信息
    作者简介:

    彭煜航(1999~),男,福建仙游人,在读硕士研究生,主要从事生理学科学研究工作

    通讯作者:

    徐世莲,E-mail:xushilian@kmmu.edu.cn

  • 中图分类号: R338.3

The Antinociception of Galanin on Nucleus Accumbens and Its Mechanism in Rats

  • 摘要:   目的  研究伏核甘丙肽对正常大鼠、炎症痛大鼠和神经痛大鼠的镇痛作用,并初步探索其机制。  方法  炎症痛动物模型采用鹿角菜碱足底皮下注射的方法制备,神经痛动物模型采用左侧坐骨神经结扎的方法制备。测量大鼠的后爪缩爪潜伏期(hind-paw withdraw latency,HWL)和后爪缩爪阈值(hind-paw withdraw threshold,HWT)来反应痛阈;使用蛋白免疫印迹法检测大鼠伏核甘丙肽受体(galanin receptors,GalRs)的表达。  结果  (1)伏核注射甘丙肽引起正常大鼠(P < 0.05;P < 0.01)、炎症痛大鼠(P < 0.001;P < 0.001)和神经痛大鼠(P < 0.001;P < 0.001)的HWL延长,且正常大鼠(P < 0.001;P < 0.01)、炎症痛大鼠(P < 0.001;P < 0.001)和神经痛大鼠(P < 0.001;P < 0.001)的HWT增加;(2)比较甘丙肽对3种大鼠的镇痛作用,甘丙肽对炎症痛大鼠(HWL:P < 0.05,P < 0.05;HWT:P < 0.01,P < 0.05)和神经痛大鼠(HWL:P < 0.05,P < 0.05;HWT:P < 0.001,P < 0.001)的镇痛作用比对正常大鼠的镇痛作用强;但比较甘丙肽对炎症痛大鼠和神经痛大鼠的镇痛作用,差异无统计学意义(P > 0.05);(3)足底皮下注射鹿角菜碱后3 h(P < 0.001)和坐骨神经结扎后14 d(P < 0.001)引起大鼠伏核神经细胞GalR1的表达上调,且注射鹿角菜碱后3 h(P < 0.05)和坐骨神经结扎后14 d(P < 0.05)大鼠伏核神经细胞GalR2的表达也上调。  结论  伏核甘丙肽对正常大鼠、炎症痛大鼠和神经痛大鼠都具有镇痛作用,甘丙肽通过和其受体结合对大鼠具有镇痛作用。
  • 图  1  伏核注射位点鉴定图

    NAc:伏核。

    Figure  1.  Illustration of the location of the injection needle tips

    图  2  伏核注射甘丙肽对正常大鼠、炎症痛大鼠和神经痛大鼠 HWL 和 HWT 的影响

    A:左 HWL、B:右 HWL、C:左 HWT、D:右 HWT。 与生理盐水对照组比较, *P < 0.05、 **P < 0.01、***P < 0.001。

    Figure  2.  Effects of intra-NAc injection of galanin on HWL and HWT in normal, inflammatory and CCI rats

    图  3  甘丙肽对正常大鼠、炎症痛大鼠和神经痛大鼠的镇痛作用比较

    与正常组比较,*P < 0.05、**P < 0.01、***P < 0.001。

    Figure  3.  Comparison of analgesic effects of galanin on native, inflammatory and CCI rats

    图  4  正常大鼠、炎症痛大鼠和神经痛大鼠 GalR1 和 GalR2 的表达

    A:Western blot 检测GalR1、GalR2和GAPDH的表达条带;B:GalR1或GalR2/GAPDH的比值图与正常组比较,*P < 0.05、***P < 0.001。

    Figure  4.  Expression of GalR1 and GalR2 in native, inflammatory and CCI rats

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  • 收稿日期:  2023-04-01
  • 网络出版日期:  2023-09-23
  • 刊出日期:  2023-09-30

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