大鼠伏核甘丙肽镇痛作用及其机制

彭煜航; 李焱娟; 曲婧红; 唐代欢; 张宏伟; 吴睿; 徐世莲

doi:10.12259/j.issn.2095-610X.S20230925

大鼠伏核甘丙肽镇痛作用及其机制

doi: 10.12259/j.issn.2095-610X.S20230925

昆明医科大学基础医学院生理学系，云南昆明　650500

基金项目: 国家自然科学基金资助项目（82160228 ）；云南省大学生创新性实验基金资助项目（2022JXD281）

详细信息

作者简介:
彭煜航（1999～），男，福建仙游人，在读硕士研究生，主要从事生理学科学研究工作

通讯作者:
徐世莲，E-mail：xushilian@kmmu.edu.cn

中图分类号: R338.3
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出版历程
- 收稿日期: 2023-04-01
- 网络出版日期: 2023-09-23
- 刊出日期: 2023-09-30

The Antinociception of Galanin on Nucleus Accumbens and Its Mechanism in Rats

Dept. of Physiology，Faculty of Basic Medical Science，Kunming Medical University，Kunming Yunnan 650500，China

摘要

摘要: 目的研究伏核甘丙肽对正常大鼠、炎症痛大鼠和神经痛大鼠的镇痛作用，并初步探索其机制。方法炎症痛动物模型采用鹿角菜碱足底皮下注射的方法制备，神经痛动物模型采用左侧坐骨神经结扎的方法制备。测量大鼠的后爪缩爪潜伏期（hind-paw withdraw latency，HWL）和后爪缩爪阈值（hind-paw withdraw threshold，HWT）来反应痛阈；使用蛋白免疫印迹法检测大鼠伏核甘丙肽受体（galanin receptors，GalRs）的表达。结果（1）伏核注射甘丙肽引起正常大鼠（P_左 < 0.05；P_右 < 0.01）、炎症痛大鼠（P_左 < 0.001；P_右 < 0.001）和神经痛大鼠（P_左 < 0.001；P_右 < 0.001）的HWL延长，且正常大鼠（P_左 < 0.001；P_右 < 0.01）、炎症痛大鼠（P_左 < 0.001；P_右 < 0.001）和神经痛大鼠（P_左 < 0.001；P_右 < 0.001）的HWT增加；（2）比较甘丙肽对3种大鼠的镇痛作用，甘丙肽对炎症痛大鼠（HWL：P_左 < 0.05，P_右 < 0.05；HWT：P_左 < 0.01，P_右 < 0.05）和神经痛大鼠（HWL：P_左 < 0.05，P_右 < 0.05；HWT：P_左 < 0.001，P_右 < 0.001）的镇痛作用比对正常大鼠的镇痛作用强；但比较甘丙肽对炎症痛大鼠和神经痛大鼠的镇痛作用，差异无统计学意义（P > 0.05）；（3）足底皮下注射鹿角菜碱后3 h（P < 0.001）和坐骨神经结扎后14 d（P < 0.001）引起大鼠伏核神经细胞GalR1的表达上调，且注射鹿角菜碱后3 h（P < 0.05）和坐骨神经结扎后14 d（P < 0.05）大鼠伏核神经细胞GalR2的表达也上调。结论伏核甘丙肽对正常大鼠、炎症痛大鼠和神经痛大鼠都具有镇痛作用，甘丙肽通过和其受体结合对大鼠具有镇痛作用。
- 甘丙肽 /
- 神经痛 /
- 炎症痛 /
- 镇痛作用 /
- 伏核
Abstract: Objective To research the analgesic effect of galanin on native rats, inflammatory rats and neuropathic rats in nucleus accumbens （NAc）, and to explore its mechanism. Methods A inflammatory pain model was prepared by subcutaneous injection of carrageine into rat’s plantar, while the model of neuralgia was prepared by ligation of the left sciatic nerve. The hind-paw withdraw latency （HWL） induced by thermal stimulation and the hind-paw withdraw threshold （HWT） induced by mechanical stimulation were measured to reflect the pain threshold. The expression of galanin receptors （GalRs） in the nucleus accumbens of inflammatory and neuropathic rats were detected by western blot （WB）. Results 1. The HWL of normal （P_left < 0.05; P_right < 0.01）, inflammatory （P_left < 0.001; P_right < 0.001） and neuropathic （P_left < 0.001; P_right < 0.001） rats were increased by injection of galanin into nucleus accumbens, and the HWT of normal （P_left < 0.001; P_right < 0.01）, inflammatory （P_left < 0.001; P_right < 0.001） and neuropathic （P_left < 0.001; P_right < 0.001） rats also were increased. 2. The antinociception of galanin on the rats with inflammatory （HWL: P_left < 0.05, P_right < 0.05; HWT: P_left < 0.01, P_right < 0.05） or neuropathic pain （HWL: P_left < 0.05, P_right < 0.05; HWT: P_left < 0.001, P_right < 0.001） was stronger than that of the native rats. However, the antinociception of galanin on the rats with the inflammatory pain and neuropathic pain was not significantly different （P > 0.05）. 3. The expressions of GalR1 were increased in rats after 3 hours of subcutaneous injection of carrageine （P < 0.001） and 14 days of sciatic nerve ligation （P < 0.001）, and the expressions of GalR2 also were increased in rats after 3 hours of subcutaneous injection of carrageine （P < 0.05） and 14 days of sciatic nerve ligation （P < 0.05）. Conclusion Intra-NAc injection of galanin has the antinociceptive effects on native, inflammatory and neuralgia rats and the antinociceptive effect of galanin in rats is induced by the activation of GalRs.
- Galanin /
- Neuralgia /
- Inflammatory pain /
- Antinociceptive effect /
- Nucleus accumbens

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图 1 伏核注射位点鉴定图

NAc：伏核。

Figure 1. Illustration of the location of the injection needle tips

下载: 全尺寸图片幻灯片

图 2 伏核注射甘丙肽对正常大鼠、炎症痛大鼠和神经痛大鼠 HWL 和 HWT 的影响

A：左 HWL、B：右 HWL、C：左 HWT、D:右 HWT。与生理盐水对照组比较， ^*P < 0.05、 ^**P < 0.01、^***P < 0.001。

Figure 2. Effects of intra-NAc injection of galanin on HWL and HWT in normal, inflammatory and CCI rats

下载: 全尺寸图片幻灯片

图 3 甘丙肽对正常大鼠、炎症痛大鼠和神经痛大鼠的镇痛作用比较

与正常组比较，^*P < 0.05、^**P < 0.01、^***P < 0.001。

Figure 3. Comparison of analgesic effects of galanin on native, inflammatory and CCI rats

下载: 全尺寸图片幻灯片

图 4 正常大鼠、炎症痛大鼠和神经痛大鼠 GalR1 和 GalR2 的表达

A：Western blot 检测GalR1、GalR2和GAPDH的表达条带；B：GalR1或GalR2/GAPDH的比值图与正常组比较，^*P < 0.05、^***P < 0.001。

Figure 4. Expression of GalR1 and GalR2 in native, inflammatory and CCI rats

下载: 全尺寸图片幻灯片

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