Analgesic Effect of Needle-knife Therapy on Rats with Neuropathic Pain
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摘要:
目的 研究针刀疗法对坐骨神经结扎所致神经痛大鼠的镇痛作用,为针刀治疗神经痛在临床的应用和推广提供实验依据。 方法 采用热板测痛仪(Hot-plate)测定伤害性热刺激诱发的大鼠后爪缩爪潜伏期(hindpaw withdrawal lantency,HWL)和压力测痛仪(Randal Selitto)测定压力刺激诱发的大鼠后爪缩爪阈值(hindpaw withdrawal threshold,HWT)作为判断痛阈的指标。左侧坐骨神经干部分结扎(chronic constriction injury,CCI)构建神经痛大鼠模型,将CCI大鼠随机分为模型组、对照组和针刀治疗组,测定各组大鼠的痛阈。CCI大鼠随机分为吗啡组(皮下注射吗啡10 mg/kg)和针刀治疗组(针刀干预),观察各组大鼠痛阈的改变。 结果 与结扎前比较,左侧坐骨神经结扎后10 d(HWL:P左 < 0.01,P右 < 0.01;HWT:P左 < 0.001,P右 < 0.001)、20 d(HWL:P左 < 0.001,P右 < 0.001;HWT:P左 < 0.001,P右 < 0.001)和30 d(HWL:P左 < 0.001,P右 < 0.05;HWT:P左 < 0.001,P右 < 0.001)引起大鼠双侧HWL和HWT 均减小。与对照组比较,针刀组大鼠的HWL(P左 < 0.001;P右 < 0.001)和HWT(P左 < 0.001;P右 < 0.001)均显著延长;与模型组比较,针刀组大鼠的HWL(P左 < 0.001;P右 < 0.001)和HWT(P左 < 0.001;P右 < 0.001)也显著延长。而模型组与对照组比较则差异无统计学意义(P > 0.05)。与吗啡组比较,针刀组对神经痛的镇痛作用稳定,且持续时间长,而皮下注射吗啡组在注射后20~180 min时,镇痛作用显著,但随着时间的延长,其镇痛作用也随之减弱甚至消失。 结论 针刀干预对CCI大鼠具有明显的镇痛作用;与吗啡镇痛作用比较,针刀治疗神经痛的作用稳定且持久。 Abstract:Objective To explore the antinociception of acupotomology in rats with sciatic nerve ligation, so as to provide experimental basis for the clinical application and promotion of needle-knife therapy on neuralgia. Methods The hot-plate test was used to measure the hindpaw withdrawal latency (HWL) induced by thermal stimuli, and the Randall-Selitto test was used to measure the hindpaw withdrawal threshold (HWT) induced by pressure stimuli as indicators of pain threshold. The chronic constriction injury (CCI) model was established by partial ligation of the left sciatic nerve to create neuropathic pain in rats. The CCI rats were randomly divided into a model group, a control group, and a needle-knife treatment group, and the pain thresholds of each group of rats were measured. The CCI rats were further randomly divided into a morphine group (subcutaneous injection of morphine 10 mg/kg) and a needle-knife treatment group (needle-knife intervention), and the pain thresholds in each group of rats were observed. Results Compared with before ligation, bilateral HWL and HWT of rats decreased after left sciatic nerve ligation at 10 d (HWL: Pleft < 0.01, Pright < 0.01; HWT: Pleft < 0.001, Pright < 0.001), 20 d (HWL: Pleft < 0.001, Pright < 0.001; HWT: Pleft < 0.001, Pright < 0.001) and 30 d (HWL: Pleft < 0.001, Pright < 0.05; HWT: Pleft < 0.001, Pright < 0.001). Compared with the control group, the HWL (left: P < 0.001; right: P < 0.001) and HWT (left: P < 0.001; right: P < 0.001) of rats in the needle-knife group were significantly prolonged; compared with the model group, the HWL (left: P < 0.001; right: P < 0.001) and HWT (left: P < 0.001; right: P < 0.001) of rats in the needle-knife group were also significantly prolonged. However, there was no significant difference between the model group and the control group (P > 0.05). Compared with the morphine group, the analgesic effect of the needle-knife group on neuropathic pain was stable and lasted longer, while the subcutaneous injection of morphine had a significant analgesic effect at 20-180 min after injection, but its analgesic effect weakened or even disappeared with the prolongation of time. Conclusion Needle-knife therapy has an analgesic effect on CCI rats. Compared with morphine, the analgesic effect of needle- knife therapy is more stable and lasting. -
Key words:
- Needle- knife /
- Neuralgia /
- Analgesic effect
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