The Effect of Probiotics on the Expression of Aβ and the Protective Effect of Neurons in Rats with Cerebral Ischemia-Reperfusion Injury
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摘要:
目的 探讨益生菌干预对脑缺血再灌注损伤大鼠脑组织Aβ表达的影响及神经元的保护作用。 方法 实验动物为雄性Sprague-Dawley大鼠，随机分为4组，各组n = 12：假手术组、模型组、益生菌组、依达拉奉组。假手术组仅分离颈总动脉，其余各组使用线拴法建立脑缺血再灌注模型。脑损伤程度使用神经行为学评分评估；大鼠脑组织梗死面积使用TTC染色观察；大鼠大脑海马CA1区与皮层区神经元病理形态学改变特征使用HE染色观察；Aβ蛋白的表达使用免疫组化实验检测。 结果 模型组相较假手术组，神经行为学评分升高（P < 0.05），脑梗死面积增加（P < 0.05），海马CA1区与皮层区神经元出现损伤，Aβ蛋白表达显著上调（P < 0.001，P < 0.05）；益生菌组、依达拉奉组与模型组相比，神经行为学评分降低（P < 0.05），脑梗死面积减小（P < 0.05），海马CA1区与皮层区神经元损伤减轻，Aβ蛋白表达水平在两组中均显著下调（P < 0.001，P < 0.05）；依达拉奉组改善更为明显。 结论 益生菌干预可下调Aβ蛋白的表达，减轻脑缺血再灌注大鼠脑组织损伤、发挥神经元保护作用。 Abstract:Objective To explore the effect of probiotics interventionon of the expression of Aβ in rats with cerebral ischemia-reperfusion and the protective effect of neurons. Methods Male SD rats were selected as the experimental animals and randomly divided into Sham Group, Model Group, Probiotics Group and Edaravone Group. The common carotid artery was separated only to the Sham Group While a cerebral ischemia-reperfusion model was eatablished to the other groups. The degree of brain injury was evaluated using neurobehavioral scoring; The infarct area of rat brain tissue was observed using TTC staining; HE staining was used to observe the pathological and morphological changes of neurons in the CA1 and cortical regions of the hippocampus in rats; The expression of Aβ proteins was detected using immunohistochemistry experiments. Results Compared with the Sham Group, the Model Group showed an increase in neurobehavioral scores （P < 0.05）, an increase in cerebral infarction focus （P < 0.05）, neuronal damage in the hippocampal CA1 and cortical regions, and a significant upregulation of Aβ protein expression （P < 0.001, P < 0.05）; Compared with the Model Group, the Probiotics Group and Edaravone Group showed a decrease in neurobehavioral scores （P < 0.05） and a reduction in cerebral infarction focus （P < 0.05）. The Probiotic Group and the Edaravone Group showed the less neuronal damage in the hippocampal CA1 and cortical regions , and the Aβ protein expression levels were significantly down regulated in both groups （P < 0.001, P < 0.05）. The improvement was more significant in Edaravone Group. Conclusion Probiotics intervention can downregulate the expression of Aβ protein, alleviate the brain tissue damage and exert neuroprotective effects in rats with cerebral ischemia-reperfusion. -
Key words:
- Probiotics /
- Cerebral ischemia-reperfusion injury /
- β amyloid /
- Edaravone
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图 1 各组大鼠脑组织梗死灶
A：假手术组；B：模型组；C：益生菌组；D：依达拉奉组。
Figure 1. Cerebral infarction focus of rats in each group
图 2 各组大鼠大脑海马CA1区与皮层区神经元病理形态学改变（×400）
A～D：大鼠海马CA1区神经元病理形态学改变；E～H：大鼠皮层区神经元病理形态学改变。
Figure 2. Pathological and morphological changes of neurons in the hippocampus CA1 and cortical regions of rats in each group （×400）
图 3 各组大鼠大脑海马CA1区与皮层区Aβ蛋白的表达
A～D：大鼠海马CA1区神经元Aβ蛋白表达情况；E～H：大鼠皮层区神经元Aβ蛋白表达情况。
Figure 3. Expression of Aβ protein in the hippocampal CA1 and cortical regions of rats in each group
图 4 各组大鼠大脑海马CA1区与皮层区Aβ蛋白的表达比较
A：大鼠大脑海马CA1区Aβ蛋白的表达统计图；B：大鼠大脑皮层区Aβ蛋白的表达统计图与模型组比较，**P < 0.05，****P < 0.001。
Figure 4. Comparison of protein expression in the hippocampal CA1 and cortical regions of rats in each groups
表 1 Zea Longa 5 级评分法评分标准
Table 1. Zea longa grade 5 scoring method scoring standard
评分 表现 0 无神经损害症状 1 梗死侧对侧前肢和前爪不能完全伸展 2 不能正常行走，行走时向梗死侧旋转 3 行走时向梗死对侧倾倒 4 无法行走和意识障碍 
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表 2 各组大鼠神经行为学评分[（$ \bar x \pm s $），分]
Table 2. Neurobehavioral scores of rats in each group[ （$ \bar x \pm s $），points]
分组 n 神经行为学评分 假手术组 12 0±0* 模型组 12 3.51±0.41 益生菌组 12 2.53±0.41* 依达拉奉组 12 2.27±0.20* 与模型组比较，*P < 0.05。
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表 3 各组大鼠脑组织梗死灶体积的比较[（$ \bar x \pm s$）%]
Table 3. Comparison of cerebral infarction focus volume of rats in each group [（$ \bar x \pm s$）%]
分组 n 脑梗死体积百分比 假手术组 3 0±0* 模型组 3 21.56±2.24 益生菌组 3 9.48±1.43* 依达拉奉组 3 6.99±1.32* 与模型组比较，*P < 0.05。
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