Toxicity Differences of Amanita Manginiana in Seven Villiages in Yunnan Province
-
摘要:
目的 对比云南省7个县村的隐花青鹅膏对小鼠肝脏和肾脏的毒性差异。 方法 将昆明小鼠随机分为7组，每组细分为6 h、3 d、7 d、14 d，4个时间点组，每个时间组包含5只小鼠。采用云南省7个县村的隐花青鹅膏进行灌胃实验，对照组（0 h）使用等量的生理盐水进行灌胃。在每次灌胃后不同时间点抽取眼球进行取血，检测血清中谷氨酰转移酶（ALT）、天门冬氨酸转氨酶（AST）、尿酸（UA）水平。同时称量小鼠肝脏和肾脏，测定其肝脏、肾脏指数，并利用HE染色观察肝、肾组织的情况。 结果 牟定县余丁村（MDYD）和禄劝县永翠村（LQYC）组的隐花青鹅膏对小鼠肝脏和肾脏造成急性损伤。在灌胃后6 h，小鼠血清生化指标开始上升，到第7天达到峰值，第14天开始下降，显示出损伤趋于缓解（P < 0.05）。而腾冲市东华村（TCDH）组和禄劝县永翠村（LQYC）组的隐花青鹅膏仅对小鼠肝脏有损害，在第14天后血清中AST、ALT和UA水平依然呈上升趋势，表明该地区的隐花青鹅膏对肝脏影响时间较长（P < 0.05）。鹤庆县磨光村（HQMG）组、南涧县德安村（NJDA）组以及牟定县高平村（MDGP）组的隐花青鹅膏对小鼠肝脏和肾脏都造成损伤，且在第14天后血清中AST、ALT和UA水平呈持续增高趋势（P < 0.05），显示出小鼠的肝脏和肾脏损害并未出现改善。 结论 云南省不同县村的隐花青鹅膏均存在不同程度的肝脏、肾脏毒性，对肝肾有潜在危害，建议在食用隐花青鹅膏时谨慎对待。 Abstract:Objective Compare the toxic effects of Amanita Manginiana from seven different regions in Yunnan Province on the livers and kidneys of mice. Methods The Kunming mice were randomly divided into 7 groups, each subgrouped into 6 h, 3 d, 7 d, and 14 d time points, with 5 mice in each time group. The experiment involved gastric lavage using Yunnan's Amanita Manginiana from 7 villages from different counties, with the control group （0 h） receiving an equivalent amount of saline lavage. Blood samples were taken from the eyes at different time points after each lavage to measure levels of alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, and uric acid （UA） in the serum. The mice's liver and kidney weights were measured, their liver and kidney indices determined, and liver and kidney tissues were observed using HE staining. Results The Amanita Manginiana from Yuding Village, Mouding County （MDYD）, and Yongcui Village, Luquan County （LQYC）, caused acute damage to the livers and kidneys of mice. Within 6 hours of gavage, the mice's serum biochemical indicators began to rise, peaking on the 7th day, and started to decline by the 14th day, indicating a trend towards recovery of the damage （P < 0.05）. However, the Amanita Manginiana from Donghua Village, Tengchong City （TCDH）, and Yongcui Village, Luquan County （LQYC）, only damaged the livers of mice. Even after the 14th day, the levels of AST, ALT, and UA in the serum continued to rise, indicating a longer-lasting impact of the local Amanita Manginiana on the liver （P < 0.05）. The Amanita Manginiana from Moguang Village, Heqing County （HQMG）, Dean Village, Nanjian County （NJDA）, and Gaoping Village, Mouding County （MDGP）, caused damage to both the livers and kidneys of mice. After the 14th day, the levels of AST, ALT, and UA in the serum continued to increase （P < 0.05）, showing that the damage to the mice's liver and kidneys did not improve. Conclusion Amanita Manginiana from different villages in Yunnan Province contains varying levels of liver and kidney toxicity, posing a potential risk to the liver and kidneys. It is advisable to exercise caution when consuming Amanita Manginiana. -
Key words:
- Amanita manginiana /
- Hepatotoxicity /
- Renal toxicity /
- Yunnan
-
图 1 隐花青鹅膏灌胃后对小鼠肝脏组织的影响（HE染色，×400）
黑色箭头显示较正常细胞；黄色箭头显示细胞出现疏松水肿；蓝色箭头显示细胞出现空泡样变。
Figure 1. Effect of Amanita manginiana on liver tissue of mice （HE staining，×400）
图 2 隐花青鹅膏灌胃后对小鼠肝脏组织的影响（HE染色，×400）
黄色箭头显示细胞出现疏松水肿；蓝色箭头显示细胞出现空泡样变;红色箭头显示近曲小管管腔内蛋白质样物质。
Figure 2. Effect of Amanita manginiana on kidney tissue of mice （HE staining，×400）
表 1 隐花青鹅膏灌胃后昆明小鼠肝脏脏器指数（$ \bar x \pm s $，n = 5）
Table 1. Liver organ index of Kunming mice after intragastric administration of Amanita manginiana（$ \bar x \pm s $，n = 5）
组别 0 min 6 h 3 d 7 d 14 d F P MDYD 5.04±0.4 4.24±0.73 5.25±0.23★▲ 5.55±0.96★ 5.09±0.31★ 6.623 0.0003 *LFGC 5.04±0.4 4.69±0.63★ 5.77±0.62★ 5.65±0.49★ 5.12±0.31▲ 6.269 0.0002 *HQMG 5.04±0.4 4.12±0.49★▲ 5.27±0.38▲ 6.02±1.05★▲ 4.57±0.56 7.270 0.0005 *TCDH 5.04±0.4 3.95±0.24★▲ 5.18±0.19▲ 5.24±0.28 4.90±0.18 14.04 < 0.0001 *MDGP 5.04±0.4 3.89±0.30★▲ 4.97±0.29▲ 5.02±0.04 4.94±0.26 11.78 < 0.0001 *NJDA 5.04±0.4 4.19±0.21★▲ 5.04±0.62▲ 4.28±0.70★▲ 5.05±0.28▲ 4.554 0.0067 *LQYC 5.04±0.4 3.86±0.24★▲ 4.46±0.29★▲ 5.15±0.36▲ 4.66±0.32▲ 11.13 < 0.0001 *F 1.000 2.339 5.991 3.632 2.649 P 0.4334 0.0508 0.0002 *0.0060 *0.0302 **P < 0.05；与0 min组比较，★P < 0.05；与同一地点的前一时间点比较，▲P < 0.05。 
下载: 导出CSV
表 2 隐花青鹅膏灌胃后昆明小鼠肾脏脏器指数（$ \bar x \pm s $，n = 5）
Table 2. Renal organ index of Kunming mice after intragastric administration of Amanita manginiana（$ \bar x \pm s $，n = 5）
组别 0 min 6 h 3 d 7 d 14 d F P MDYD 1.09±0.08 1.08±0.09 1.14±0.07 1.13±0.18 1.16±0.07 0.6203 0.6496 LFGC 1.09±0.08 1.17±0.15 1.17±0.13 1.18±0.11★ 1.11±0.11 1.666 0.1744 HQMG 1.09±0.08 0.94±0.16 0.96±0.05 1.14±0.22▲ 0.90±0.02★▲ 3.518 0.0207 *TCDH 1.09±0.08 0.96±0.04★▲ 1.00±0.11 0.99±0.06 1.01±0.06 1.526 0.2249 MDGP 1.09±0.08 1.06±0.10 1.00±0.07 1.03±0.03 1.01±0.05 0.8217 0.5237 NJDA 1.09±0.08 0.99±0.07 0.95±0.06★ 0.82±0.12★▲ 0.94±0.09★▲ 6.629 0.0009 *LQYC 1.09±0.08 1.02±0.03 1±0.07 0.92±0.02★▲ 0.96±0.02★ 5.241 0.0033 *F 1.000 3.868 6.619 5.540 10.87 P 0.4334 0.0041 *< 0.0001 *0.0003 *< 0.0001 **P < 0.05；与MDYD组比较，与0 min组比较，★P < 0.05；与同一地点的前一时间点比较，▲P < 0.05。
下载: 导出CSV
表 3 云南不同县村的隐花青鹅膏灌胃后不同时间点血清AST、ALT和UA的变化（$ \bar x \pm s $，n = 5）
Table 3. Changes in serum AST，ALT，and UA at different time points after intragastric administration of Amanita manginiana in different regions of Yunnan（$ \bar x \pm s $，n = 5）
组别 生化指标 0 min 6 h 3 d 7 d 14 d F P MDYD AST（U/L） 102.69±4.14 120.99±4.62eg★▲ 123.94±5.51d★▲ 145.43±2.79cdefg★ 132.39±3.77bd★▲ 95.05 < 0.0001 *ALT（U/L） 66.8±3.3 76.96±2.63★▲ 84.31±2.16deg★ 90.66±1.64cdefg★ 75.07±3.48bcdeg★ 82.19 < 0.0001 *UA（μmol/L） 17.1±0.7 19.43±0.68deg★▲ 21.32±0.61deg★▲ 21.32±0.68cdefg★ 17.99±0.79bef★▲ 50.11 < 0.0001 *LFGC AST（U/L） 102.69±4.14 120.53±5.46eg★▲ 131.05±6.07cd★ 137.28±3.42acdf★▲ 110.49±3.97adeg★▲ 62.44 < 0.0001 *ALT（U/L） 66.8±3.3 79.33±2.51★▲ 85.21±2.79deg★▲ 87.43±2.2adef★▲ 85.59±2.91adf★▲ 68.32 < 0.0001 *UA（μmol/L） 17.1±0.7 19.6±0.71deg★▲ 21.36±1.02deg★▲ 21.58±0.7cdefg★ 19.94±0.81ag★▲ 41.54 < 0.0001 *HQMG AST（U/L） 102.69±4.14 114.56±4.35eg★▲ 116.85±5.71bdf★ 109.17±6.54abd★▲ 122.7±5.68de★▲ 15.34 < 0.0001 *ALT（U/L） 66.8±3.3 77.34±2.85★▲ 80.83±3.43d★ 86.05±3.23def★▲ 93.01±3.02abfg★▲ 66.90 < 0.0001 *UA（μmol/L） 17.1±0.7 19.62±0.86dg★▲ 21.29±0.68deg★▲ 16.73±0.78abe▲ 19.2±0.72e★▲ 37.54 < 0.0001 *TCDH AST（U/L） 102.69±4.14 122.62±4.5eg★▲ 137.56±2.97aceg★▲ 153.01±4.68bcefg★▲ 150.22±4.32abcfg★ 182.2 < 0.0001 *ALT（U/L） 66.8±3.3 83.65±4.65★▲ 95.93±3.85abcefg★▲ 100.72±1.75abcefg★▲ 99.39±2.23abfg★ 139.9 < 0.0001 *UA（μmol/L） 17.1±0.7 16.03±1.13abcef*▲ 16.39±0.89abcef 17.65±0.85abe▲ 17.62±1.02ef 3.378 0.0243 *MDGP AST（U/L） 102.69±4.14 106.98±4.4abdf★ 121.97±5.24d★▲ 128.95±6.49cdf★▲ 141.33±4.07bcfg★▲ 68.84 < 0.0001 *ALT（U/L） 66.8±3.3 72±4.44abcdg▲ 75.42±2.64abcd★ 73.52±3.68abdg★ 98.22±3.84abcfg★▲ 67.13 < 0.0001 *UA（μmol/L） 17.1±0.7 17.34±0.83abcdf 18.32±0.67abcdfg★▲ 20.18±0.38abcdfg★▲ 21.49±0.77acdg★▲ 44.35 < 0.0001 *NJDA AST（U/L） 102.69±4.14 126.03±3.99abcdf★▲ 134.46±5.28cg★▲ 109.21±6.03abde★▲ 121.83±4.97bde★▲ 47.70 < 0.0001 *ALT（U/L） 66.8±3.3 75.83±2.68★▲ 82.2±1abcdg★▲ 71.8±1.99cde★▲ 78.55±3.45abcdrg★▲ 32.04 < 0.0001 *UA（μmol/L） 17.1±0.7 19.58±0.28dg★▲ 21.69±0.98dfg★▲ 17.24±0.75abe▲ 20.87±0.99adg★▲ 47.66 < 0.0001 *LQYC AST（U/L） 102.69±4.14 102.82±3.61abce 115.86±5.96bdf★▲ 118.42±6.62abd★ 125.16±3.84bde★▲ 25.94 < 0.0001 *ALT（U/L） 66.8±3.3 76.82±2.52★▲ 77.42±2.76df 81.62±3.73acde★▲ 82.95±2.49acdef★ 33.30 < 0.0001 *UA（μmol/L） 17.1±0.7 16.2±0.98abcf★▲ 15.88±0.76abcef★ 16.51±0.54abe 17.24±0.8bef 3.411 0.0234 *AST
（U/L）F 1.000 18.62 13.04 71.70 63.30 P 0.4334 < 0.0001 *< 0.0001 *< 0.0001 *< 0.0001 *ALT
（U/L）F 1.000 6.697 30.77 65.88 57.98 P 0.4334 < 0.0001 *< 0.0001 *< 0.0001 *< 0.0001 *UA
（μmol/L）F 1.000 25.89 54.69 72.43 21.78 P 0.4334 < 0.0001 *< 0.0001 *< 0.0001 *< 0.0001 *与MDYD组比较，aP < 0.05；与LFGC比较，bP < 0.05；与HQMG比较，cP < 0.05；与TCDH比较，dP < 0.05；与MDGP比较，eP < 0.05；与NJDA比较，fP < 0.05；与LQYC比较，gP < 0.05。与0 min组比较，★P < 0.05；与同一地点的前一时间点比较，▲P < 0.05；*P < 0.05。
下载: 导出CSV
-
[1] 杨祝良. 试谈我国鹅膏菌的分类研究[J]. 菌物系统,2000,19(3):435-440. [2] 杨祝良. 我国的几种剧毒鹅膏菌[J]. 中国食用菌,2002,21(3):17-18. doi: 10.3969/j.issn.1003-8310.2002.03.009 [3] Berger K J,Guss D A. Mycotoxins revisited: Part II[J]. J Emerg Med,2005,28(2):175-183. doi: 10.1016/j.jemermed.2004.08.019 [4] 董国日,张志光,陈作红. 鹅膏菌毒素及其毒理研究进展[J]. 生物学杂志,2000,17(3):1-3. doi: 10.3969/j.issn.2095-1736.2000.03.001 [5] 颜芝闻,吴晓旻,裴红兵,等. 武汉市一起毒蘑菇中毒事件现场调查和鉴定[J]. 中国食品卫生杂志,2020,32(5):566-569. [6] 李海蛟,章轶哲,刘志涛,等. 云南蘑菇中毒事件中的毒蘑菇物种多样性[J]. 菌物学报,2022,41(9):1416-1429. [7] 杜进良,瞿鹏飞,李玉华,等. 云南不明原因猝死地区隐花青鹅膏的毒性研究[J]. 中国法医学杂志,2022,37(1):54-59. [8] 何永旺,赵祥月,贾彭林,等. 云南不明原因猝死研究进展[J]. 法医学杂志,2017,33(5):470-475. doi: 10.3969/j.issn.1004-5619.2017.05.003 [9] 陈丽,邢琴,周忠志,等. 复方ANBP对小鼠创面组织学变化的实验研究[J]. 湖南中医药大学学报,2019,39(6):699-703. doi: 10.3969/j.issn.1674-070X.2019.06.005 [10] Nair A,Morsy M A,Jacob S. Dose translation between laboratory animals and human in preclinical and clinical phases of drug development[J]. Drug Dev Res,2018,79(8):373-382. doi: 10.1002/ddr.21461 [11] 黄娜娜,郭欣,蔡涛涛,等. 决明子不同提取物单次给药对小鼠肝、肾“量-时-毒”关系研究[J]. 中国药物警戒,2017,14(10):594-598. doi: 10.3969/j.issn.1672-8629.2017.10.005 [12] Ming L,Qi B,Hao S,et al. Camel milk ameliorates inflammatory mechanisms in an alcohol-induced liver injury mouse model[J]. Sci Rep,2021,11(1):22811. doi: 10.1038/s41598-021-02357-1 [13] 陈瑜鑫,李瑶,高海云,等. 鹅膏菌毒性成分中毒特征及开发应用研究进展[J]. 中国药物警戒,2024,21(5):496-501. [14] Hu W,Li Z,Wang W,et al. Structural characterization of polysaccharide purified from Amanita caesarea and its pharmacological basis for application in Alzheimer’s disease: Endoplasmic reticulum stress[J]. Food Funct,2021,12(21):11009-11023. doi: 10.1039/D1FO01963E [15] Sun L,Liu Q,Bao C,et al. Comparison of free total amino acid compositions and their functional classifications in 13 wild edible mushrooms[J]. Molecules,2017,22(3):350 doi: 10.3390/molecules22030350 [16] Surayot U,Wangtueai S,You S,et al. Sulphation and hydrolysis improvements of bioactivities,and immuno-modulatory properties of edible amanita hemibapha subspecies javanica (corner and bas) mucilage polysaccharide as a potential in personalized functional foods[J]. J Fungi (Basel),2021,7(10):847. doi: 10.3390/jof7100847 [17] 张慧,陈蕊,陈霞,等. 世界野生食用菌产业经济发展研究概述[J]. 经济研究导刊,2014(28):59-61,75. doi: 10.3969/j.issn.1673-291X.2014.28.023 [18] 卯晓岚. 中国毒菌物种多样性及其毒素[J]. 菌物学报,2006,25(3):345-363. doi: 10.3969/j.issn.1672-6472.2006.03.004 [19] 母华强,陈艳. 珠海城区臭鼩身体大小和脏器指数的性别及季节差异[J]. 野生动物学报,2022,43(2):386-393. doi: 10.12375/ysdwxb.20220212 [20] 王磊,赵鹏. 拉米夫定联合恩替卡韦对乙型肝炎患者肝功能及Th1/Th2类细胞因子的影响[J]. 检验医学与临床,2019,16(15):2226-2228. doi: 10.3969/j.issn.1672-9455.2019.15.037 [21] 王如萍,刘梦姣,田梦新,等. 败酱草对小鼠急性酒精性肝损伤作用的研究[J]. 中医临床研究,2023,15(20):137-142. doi: 10.3969/j.issn.1674-7860.2023.20.027 [22] 苗志敏,赵世华,王颜刚,等. 山东沿海居民高尿酸血症及痛风的流行病学调查[J]. 中华内分泌代谢杂志,2006,22(5):421-425. doi: 10.3760/j.issn:1000-6699.2006.05.005 [23] Danese E,Montagnana M. An historical approach to the diagnostic biomarkers of acute coronary syndrome[J]. Ann Transl Med,2016,4(10):194. doi: 10.21037/atm.2016.05.19 [24] 龙武,瞿鹏飞,马琳,等. 一起云南不明原因猝死案件中4种野生菌的细胞毒性[J]. 法医学杂志,2023,39(2):121-128. -
点击查看大图
图(2) / 表(3)
计量
- 文章访问数: 783
- HTML全文浏览量: 646
- PDF下载量: 9
- 被引次数: 0
