半枝莲水提物通过调节免疫微环境抑制乳腺癌肺转移

廖泉炀; 任安吕; 左晨蓉; 李菊; 孙瑞芬; 苏晓三

doi:10.12259/j.issn.2095-610X.S20250703

半枝莲水提物通过调节免疫微环境抑制乳腺癌肺转移

doi: 10.12259/j.issn.2095-610X.S20250703

廖泉炀^1,,
任安吕¹,
左晨蓉²,
李菊³,
孙瑞芬^2, ,,
苏晓三^4, ,

1.
云南中医药大学中药学院
2.
护理学院
3.
基础医学院
4.
科研实验中心，云南昆明　650500

基金项目: 国家自然科学基金地区科学基金（82060519）；云南省中医药基础研究联合专项基金（202301AZ070001-062）；云南省应用基础研究中医联合专项基金（2019FF002（-011））；云南省教育厅科学研究基金（2024Y355）

详细信息

作者简介:
廖泉炀（1995 ～），男，贵州遵义人，在读硕士研究生，主要从事乳腺癌的发病机制研究工作

通讯作者:
孙瑞芬，E-mail：sunruifen@ynucm.edu.cn

苏晓三，E-mail：suxs163@163.com

中图分类号: R73-37
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- 被引次数: 0
出版历程
- 收稿日期: 2025-04-21
- 刊出日期: 2025-07-21

Scutellaria barbata D.Don Water Extract Inhibits Breast Cancer Lung Metastasis by Modulating the Immune Microenvironment

Quanyang LIAO^1
,,
Anlv REN¹,
Chenrong ZUO²,
Jv LI³,
Ruifen SUN^{2
, ,},
Xiaosan SU^{4
, ,}

1.
College of Traditional Chinese Medicine
2.
School of Nursing
3.
School of Basic Medical Sciences
4.
Scientific Research and Experimental Center，Yunnan University of Chinese Medicine，Kunming Yunnan 650500，China

摘要

摘要: 目的探讨半枝莲水提物（Scutellaria barbata D.Don water extract，SBW）通过调节免疫微环境抑制乳腺癌肺转移的作用机制。方法利用4T1乳腺癌细胞建立小鼠肺转移模型，将小鼠分为对照组（n = 6）和SBW治疗组（n = 6）。通过测量原位瘤体积评估SBW对肿瘤生长的影响，并采用H&E染色观察肺组织中转移结节的数量和面积，以检测其对肺转移的抑制作用。使用流式细胞术分析肿瘤、外周血和肺组织中免疫细胞的组成变化。结果与对照组相比，SBW治疗组抑制了原位瘤的生长（P < 0.01），并减少了肺转移结节的数量和面积（P < 0.01）。流式细胞术分析显示，SBW治疗后，肿瘤组织中CD86⁺巨噬细胞（P < 0.001）和粒细胞样髓系抑制细胞（polymorphonuclear myeloid-derived suppressor cells，PMN-MDSCs）数量增加（P < 0.05）；肺组织中CD86⁺巨噬细胞、自然杀伤T细胞（natural killer T cells，NKT）、自然杀伤细胞（natural killer cells，NK）和PMN-MDSCs数量均增加（P < 0.05）。同时，肿瘤和肺组织中的调节性T细胞（regulatory T cells，Tregs）（P < 0.05）、CD206⁺巨噬细胞（P < 0.01）和单核样髓系抑制细胞（monocytic myeloid-derived suppressor cells，M-MDSCs）（P < 0.05）数量降低。结论半枝莲水提物（SBW）通过调节肿瘤、肺组织和外周血中的免疫细胞募集与分布，增强抗肿瘤免疫反应并减弱免疫抑制，从而抑制乳腺癌的生长和肺转移。
- 半枝莲 /
- 乳腺癌 /
- 肺转移 /
- 免疫微环境
Abstract: Objective To investigate the mechanism by which Scutellaria barbata D. Don water extract （SBW） inhibits lung metastasis of breast cancer by regulating the immune microenvironment. Methods A mouse model of lung metastasis was established using 4T1 breast cancer cells. Mice were divided into a control group （n = 6） and an SBW treatment group （n = 6）. The effect of SBW on tumor growth was assessed by measuring the volume of the primary tumor, and the inhibitory effect on lung metastasis was evaluated by observing the number and area of metastatic nodules in lung tissue using H&E staining. Flow cytometry was used to analyze changes in the composition of immune cells in the tumor, peripheral blood, and lung tissue. Results Compared with the control group, the SBW treatment group inhibited the growth of the primary tumor （P < 0.01） and reduced the number and area of lung metastatic nodules （P < 0.01）. Flow cytometry analysis showed that after SBW treatment, the numbers of CD86⁺ macrophages （P < 0.001） and polymorphonuclear myeloid-derived suppressor cells （PMN-MDSCs）（P < 0.05） in the tumor tissue were increased. In lung tissue, the numbers of CD86⁺ macrophages, natural killer T （NKT） cells, natural killer （NK） cells, and PMN-MDSCs were also elevated （P < 0.05）. Meanwhile, the numbers of regulatory T cells （Tregs）（P < 0.05）, CD206⁺ macrophages （P < 0.01）, and monocytic myeloid-derived suppressor cells （M-MDSCs）（P < 0.05） in both tumor and lung tissues were decreased. Conclusion SBW inhibits the breast cancer growth and lung metastasis by regulating the recruitment and distribution of immune cells in the tumor, peripheral blood, and lung tissue, thereby enhancing anti-tumor immune responses and reducing immune suppression.
- Scutellaria barbata D. Don /
- Breast cancer /
- Lung metastasis /
- Immune microenvironment.

HTML全文

图 1 SBW对4T1荷瘤小鼠原位瘤生长和乳腺癌肺转移的抑制作用（$\bar x \pm s $，n = 6）

A：半枝莲治疗期间4T1荷瘤小鼠肿瘤体积的变化。B，C：实验结束时切除的肿瘤及其相应重量的统计图。D：实验结束时小鼠肺组织的H&E染色图像（原始放大倍数×40；比例尺 = 50 μm）。E，F：肺转移结节的量化：乳腺癌肺转移面积和肺结节数量的统计结果。G：小鼠最终肺重量的统计图。H：半枝莲治疗过程中4T1荷瘤小鼠的体重变化情况。I，J：心脏、肝脏、脾脏和肾脏的器官重量及其H&E染色图像（原始放大倍数×40；比例尺 = 50 μm）。与Control组相比：ns：P > 0.05，^**P < 0.01，^***P < 0.001。

Figure 1. SBW inhibits the growth of in situ tumors and lung metastasis of breast cancer in 4T1 tumor-bearing mice （$\bar x \pm s $，n = 6）

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图 2 SBW对小鼠肿瘤组织免疫微环境的影响（$\bar x \pm s $，n = 6）

A-C：流式细胞术检测肿瘤组织中CD3⁺ T细胞、CD3⁺CD4⁺ T细胞、CD3⁺CD8⁺ T细胞、CD4⁺CD25⁺FoxP3⁺ Treg细胞、CD11b⁺CD11c⁺树突状细胞、F4/80⁺CD86⁺（M1）和F4/80⁺CD206⁺（M2）型巨噬细胞、CD3⁺CD49b⁺NKT细胞、CD3^-CD49b⁺NK细胞以及Ly-6C^lowLy-6G⁺多形核（PMN-MDSCs）和Ly-6C⁺Ly-6G^-单核样MDSCs（M-MDSCs）。与对照组（Control）相比：ns：无显著性差异，^*P < 0.05，^**P < 0.01，^***P < 0.001。

Figure 2. Effect of SBW on the immune microenvironment in mouse tumor tissue （$\bar x \pm s $，n = 6）

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3 SBW对小鼠肺组织免疫微环境的影响（1）（$\bar x \pm s $，n = 6）

3. Effect of SBW on the immune microenvironment in mouse lung tissue （1）（$\bar x \pm s $，n = 6）

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3 SBW对小鼠肺组织免疫微环境的影响（2）（$\bar x \pm s $，n = 6）

A-C：流式细胞术检测肺组织中CD3⁺ T细胞、CD3⁺CD4⁺ T细胞、CD3⁺CD8⁺ T细胞、CD4⁺CD25⁺FoxP3⁺ Treg细胞、CD11b⁺CD11c⁺树突状细胞、F4/80⁺CD86⁺（M1）和F4/80⁺CD206⁺（M2）型巨噬细胞、CD3⁺CD49b⁺NKT细胞、CD3^-CD49b⁺NK细胞以及Ly-6C^lowLy-6G⁺多形核（PMN-MDSCs）和Ly-6C⁺Ly-6G^-单核样MDSCs（M-MDSCs）。与对照组（Control）相比：ns：无显著性差异，^*P < 0.05，^**P < 0.01。

3. Effect of SBW on the immune microenvironment in mouse lung tissue （2）（$\bar x \pm s $，n = 6）

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图 4 SBW对小鼠外周血免疫细胞的影响（$\bar x \pm s $，n = 6）

A-C：流式细胞术检测肿瘤组织中CD3⁺ T细胞、CD3⁺CD4⁺ T细胞、CD3⁺CD8⁺ T细胞、CD4⁺CD25⁺FoxP3⁺ Treg细胞、CD11b⁺CD11c⁺树突状细胞、F4/80⁺CD86⁺（M1）和F4/80⁺CD206⁺（M2）型巨噬细胞、CD3⁺CD49b⁺NKT细胞、CD3^-CD49b⁺NK细胞以及Ly-6C^lowLy-6G⁺多形核（PMN-MDSCs）和Ly-6C⁺Ly-6G^-单核样MDSCs（M-MDSCs）。与对照组（Control）相比：ns：无显著性差异，^*P < 0.05，^**P < 0.01，^***P < 0.001。

Figure 4. Effect of SBW on the immune microenvironment in mouse blood （$\bar x \pm s $，n = 6）

下载: 全尺寸图片幻灯片

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