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RSS2011 Vol. 32, No. 03
2011, 32(03)
Abstract:
Objective To establish a lentiviral vector-mediated RNA interference(RNAi)of Human Papillomavirus(HPV)16 E6 gene.Methods Using genetic recombination technology,a pair of complementary small hairpin RNA(shRNA)oligonucleotides targeting the HPV16 E6 gene was cloned into lentiviral expression vector pGCL-GFP,which contained U6 promoter and green fluorescent protein reporter.The recombined plasmid named pGCL-GFP-vshHPV 16 E6 was confirmed by polymerase chain reaction and sequencing,and was cotransfected with pH...
Objective To establish a lentiviral vector-mediated RNA interference(RNAi)of Human Papillomavirus(HPV)16 E6 gene.Methods Using genetic recombination technology,a pair of complementary small hairpin RNA(shRNA)oligonucleotides targeting the HPV16 E6 gene was cloned into lentiviral expression vector pGCL-GFP,which contained U6 promoter and green fluorescent protein reporter.The recombined plasmid named pGCL-GFP-vshHPV 16 E6 was confirmed by polymerase chain reaction and sequencing,and was cotransfected with pH...
2011, 32(03)
Abstract:
Objective To discuss the value of four experimental methods of diagnosis ED,including Intracavernous Injection(ICI),Nocturnal Penile tumescence Testing(NPT),Audio Visual Sexual Stimulation(AVSS)and Penile Sympathetic Skin Responses(PSSR),which were used in the medicolegal identification of traumatic erectile dysfunction(ED),and discuss the assessment of impairment of traumatic ED.Method The data of 50 medicolegally indentified traumatic ED cases caused by traffic accidents were collected and analyzed with s...
Objective To discuss the value of four experimental methods of diagnosis ED,including Intracavernous Injection(ICI),Nocturnal Penile tumescence Testing(NPT),Audio Visual Sexual Stimulation(AVSS)and Penile Sympathetic Skin Responses(PSSR),which were used in the medicolegal identification of traumatic erectile dysfunction(ED),and discuss the assessment of impairment of traumatic ED.Method The data of 50 medicolegally indentified traumatic ED cases caused by traffic accidents were collected and analyzed with s...
2011, 32(03)
Abstract:
Objective To study the effect of brain retraction on local biochemical metabolism in brain tissue and to investigate the potential application of microdialysis in monitoring local cerebral metabolic and biochemical changes caused by brain retraction injury.Methods Depending on different traction pressure(20 mmHg,30 mmHg,40 mmHg)and different traction mode(intermittent and continuous traction),the brain tissues were tracted.Microdialysis was applied for the dynamic collection of extracellular fluid(ECF)of di...
Objective To study the effect of brain retraction on local biochemical metabolism in brain tissue and to investigate the potential application of microdialysis in monitoring local cerebral metabolic and biochemical changes caused by brain retraction injury.Methods Depending on different traction pressure(20 mmHg,30 mmHg,40 mmHg)and different traction mode(intermittent and continuous traction),the brain tissues were tracted.Microdialysis was applied for the dynamic collection of extracellular fluid(ECF)of di...
2011, 32(03)
Abstract:
Objective To study the changes of CD45RO+ and CD45RA+T lymphocytess in peripheral blood in children with infectious mononucleosis.Method The expression of CD45RO+ and CD45RA+T lymphocytess in peripheral blood of children patients and controls was detected by flow cytometry.Results Compared with the control,the expression of CD4+CD45RO+ T lymphocytess in children with infectious mononucleosis in acute stage obviously increased(P<0.05),but the expression of CD4+CD45RA+ T lymphocytes was lower(P<0.05).The expr...
Objective To study the changes of CD45RO+ and CD45RA+T lymphocytess in peripheral blood in children with infectious mononucleosis.Method The expression of CD45RO+ and CD45RA+T lymphocytess in peripheral blood of children patients and controls was detected by flow cytometry.Results Compared with the control,the expression of CD4+CD45RO+ T lymphocytess in children with infectious mononucleosis in acute stage obviously increased(P<0.05),but the expression of CD4+CD45RA+ T lymphocytes was lower(P<0.05).The expr...
2011, 32(03)
Abstract:
Objective To investigate the application of internal quality control with two control materials in analysis of thyroid hormone by radioimmunoassay(RIA),in order to effectively reduce losing of control in testing results,by which to afford accurate and authentic test information for clinic.Methods Radioimmunoassay was used in this study.Non-fixed value control material which was self-regulated with batch number P20090723 and fixed value control materials from the three venders were used to carry on internal ...
Objective To investigate the application of internal quality control with two control materials in analysis of thyroid hormone by radioimmunoassay(RIA),in order to effectively reduce losing of control in testing results,by which to afford accurate and authentic test information for clinic.Methods Radioimmunoassay was used in this study.Non-fixed value control material which was self-regulated with batch number P20090723 and fixed value control materials from the three venders were used to carry on internal ...
2011, 32(03)
Abstract:
Objective To compare the effects of exercise and high-fat diets on body weight and metabolism in rats.Methods Immature rats were given high-fat diets(fish oil,palm oil,or soybean oil)for weeks,and then were divided randomly into 2 groups:rest group and exercise group.Rats in exercise group were given swimming exercise for 6 weeks,and were sacrificed 24 h after the last swimming exercise.The body weight,the serum levels of leptin,insulin,glucose,and I/G ratio were measured.Results High-fat diets with soybean...
Objective To compare the effects of exercise and high-fat diets on body weight and metabolism in rats.Methods Immature rats were given high-fat diets(fish oil,palm oil,or soybean oil)for weeks,and then were divided randomly into 2 groups:rest group and exercise group.Rats in exercise group were given swimming exercise for 6 weeks,and were sacrificed 24 h after the last swimming exercise.The body weight,the serum levels of leptin,insulin,glucose,and I/G ratio were measured.Results High-fat diets with soybean...
2011, 32(03)
Abstract:
Objective To analyze changing regularity of bone scan review in lung cancer patients at different intervals,and to investigate the best review time of bone scan.Methods 127 patients with primary lung cancer,a total of 346 trips bone scan results,were reviewed.According to the first bone scan,all the patients were divided into normal and abnormal group.The changes of bone scan at different intervals were compared and analyzed between the two groups.Results 61 cases(48.0%)in 127 patients with lung cancer were...
Objective To analyze changing regularity of bone scan review in lung cancer patients at different intervals,and to investigate the best review time of bone scan.Methods 127 patients with primary lung cancer,a total of 346 trips bone scan results,were reviewed.According to the first bone scan,all the patients were divided into normal and abnormal group.The changes of bone scan at different intervals were compared and analyzed between the two groups.Results 61 cases(48.0%)in 127 patients with lung cancer were...
2011, 32(03)
Abstract:
Objective Combining with patients's lipid levels,to evaluate carotid artery atherosclerosis(CAS)in patients with rheumatioid arthritis by ultrasonography.Methods The carotidarteries were assessed by ultrasonography in 16 RA patients with high lipid levels(RA1 group),23 RA patients with nomal lipid levels(RA2 group)and 20 healthy volunteers(normal control group).IMT,Dd,PSV,EDV and RI of common carotid arteries were measured by ultrasonography in the three groups.Results IMT of RA1 and RA2 patients were thick...
Objective Combining with patients's lipid levels,to evaluate carotid artery atherosclerosis(CAS)in patients with rheumatioid arthritis by ultrasonography.Methods The carotidarteries were assessed by ultrasonography in 16 RA patients with high lipid levels(RA1 group),23 RA patients with nomal lipid levels(RA2 group)and 20 healthy volunteers(normal control group).IMT,Dd,PSV,EDV and RI of common carotid arteries were measured by ultrasonography in the three groups.Results IMT of RA1 and RA2 patients were thick...
2011, 32(03)
Abstract:
Objective To investigate the correlations between coulmon allergens causing allergic dermatosis and the airborne pollen species in Kunming,in order to provide a scientific evidence for the preventing and treatment of allergic dermatosis.Methods Pollen allergens of 96 allergic dermatosis patients were detected by intradermal test.The positive rate of each kind of allergen was calculated.Samples of airborne pollen particles were collected in Kunming from Jannary 1,2007 to December 31,2007 By Durham gravity me...
Objective To investigate the correlations between coulmon allergens causing allergic dermatosis and the airborne pollen species in Kunming,in order to provide a scientific evidence for the preventing and treatment of allergic dermatosis.Methods Pollen allergens of 96 allergic dermatosis patients were detected by intradermal test.The positive rate of each kind of allergen was calculated.Samples of airborne pollen particles were collected in Kunming from Jannary 1,2007 to December 31,2007 By Durham gravity me...
2011, 32(03)
Abstract:
Objective To study the application of fiberoptic bronchoscopy brush cytology diagnosis of lung cancer.Methods We collected the data of 399 lung cancer patients diagnosed by fiberoptic bronchoscopy brush cytology in 2009,and compared to other data of the bronchofiberoscopic biopsy.Results The sensitivity of the fiberoptic bronchoscopy brush cytology in diagnosis of lung cancer was 81.7%,specificity was 98.9%,coincidence rate was 85.1%,all in biopsy in diagonis of lung cancer,the difference is significant in ...
Objective To study the application of fiberoptic bronchoscopy brush cytology diagnosis of lung cancer.Methods We collected the data of 399 lung cancer patients diagnosed by fiberoptic bronchoscopy brush cytology in 2009,and compared to other data of the bronchofiberoscopic biopsy.Results The sensitivity of the fiberoptic bronchoscopy brush cytology in diagnosis of lung cancer was 81.7%,specificity was 98.9%,coincidence rate was 85.1%,all in biopsy in diagonis of lung cancer,the difference is significant in ...
2011, 32(03)
Abstract:
Objective To discuss the incidence of acute kindey injury(AKI)in acute decompensated heart failure(ADHF)and the influencing factors.Methods We retrospectively analyzed the clinical data of 96 ADHF patients,and analyzed the incidence of AKI,and divide the patients into AKI group and no AKI group.Then we analyzed the age,sex,systolic pressure,caidiac troponin,left ventricular ejection fraction,hemoglobin and primary disease of patients in the two groups.Results In 96 ADHF patients,AKI was found in 74 patients...
Objective To discuss the incidence of acute kindey injury(AKI)in acute decompensated heart failure(ADHF)and the influencing factors.Methods We retrospectively analyzed the clinical data of 96 ADHF patients,and analyzed the incidence of AKI,and divide the patients into AKI group and no AKI group.Then we analyzed the age,sex,systolic pressure,caidiac troponin,left ventricular ejection fraction,hemoglobin and primary disease of patients in the two groups.Results In 96 ADHF patients,AKI was found in 74 patients...
2011, 32(03)
Abstract:
Objective To study the therapeutic effects of ulinastatin(UST)combined with Xuebijing on acute pancreatitis(AP)complicated with acute lung injury(ALI).Methods We collected 120 patients with APALI and divided them into four groups:UST + Xuebijing therapy group,UST therapy group,Xuebijing therapy group and control group.30 patients in control group were treated with the conventional treatment,and 30 patients in UST therapy group were treated with the conventional treatments and UST,and 30 patients in Xuebijin...
Objective To study the therapeutic effects of ulinastatin(UST)combined with Xuebijing on acute pancreatitis(AP)complicated with acute lung injury(ALI).Methods We collected 120 patients with APALI and divided them into four groups:UST + Xuebijing therapy group,UST therapy group,Xuebijing therapy group and control group.30 patients in control group were treated with the conventional treatment,and 30 patients in UST therapy group were treated with the conventional treatments and UST,and 30 patients in Xuebijin...
2011, 32(03)
Abstract:
Objective To study the children's Henoch-Schonlein purpura with headache of central nervous system damage.Methods In 117 cases of children's Henoch-Schonlein purpura,57 cases with headache were selected as headache group,60 cases without headaches were as control group,both groups underwent EEG inspection.Results EEG abnormalities were found in 48 cases in headache group,and 29 cases in control group.The difference between two groups was significant(P < 0.05).Conclusion If EEG check is underwent for the chi...
Objective To study the children's Henoch-Schonlein purpura with headache of central nervous system damage.Methods In 117 cases of children's Henoch-Schonlein purpura,57 cases with headache were selected as headache group,60 cases without headaches were as control group,both groups underwent EEG inspection.Results EEG abnormalities were found in 48 cases in headache group,and 29 cases in control group.The difference between two groups was significant(P < 0.05).Conclusion If EEG check is underwent for the chi...
2011, 32(03)
Abstract:
Objective To analyze the clinical features of the adults allergic purpura.Methods The clinical and laboratorial data of 32 in-patients with adults allergic purpura in the third people's Hosptial Yunnan Province,between Jan 1990 and Jan 2010,were retrospectively collected and analyzed respectively.Results Most patients with adults allergic purpura in this group were male and young adult.Adults allergic purpura occurred more commonly in the winter and the spring.The important predisposing factors were upper r...
Objective To analyze the clinical features of the adults allergic purpura.Methods The clinical and laboratorial data of 32 in-patients with adults allergic purpura in the third people's Hosptial Yunnan Province,between Jan 1990 and Jan 2010,were retrospectively collected and analyzed respectively.Results Most patients with adults allergic purpura in this group were male and young adult.Adults allergic purpura occurred more commonly in the winter and the spring.The important predisposing factors were upper r...
2011, 32(03)
Abstract:
Objective To investigate whether Chinese herbal medicine combined with antifungal therapy can effectively improve long-term prognosis of recurrent vulvovaginal candidiasis(RVVC),and to observe the local cellular immunity changes of vagina during the treatment.Methods 104 patients with RVVC from February 2006 to December 2008 were included in the study.They were randomly distributed into two groups: combined treatment group and the control group.Each group consisted of 52 cases.All the patients received anti...
Objective To investigate whether Chinese herbal medicine combined with antifungal therapy can effectively improve long-term prognosis of recurrent vulvovaginal candidiasis(RVVC),and to observe the local cellular immunity changes of vagina during the treatment.Methods 104 patients with RVVC from February 2006 to December 2008 were included in the study.They were randomly distributed into two groups: combined treatment group and the control group.Each group consisted of 52 cases.All the patients received anti...
2011, 32(03)
Abstract:
Objective To explore the treatments of colonic injuries complicating multi-organ or multi-locum injuries.Methods 16 cases with colonic injuries complicating multi-organ or multi-locum injuries were analyzed retrospectively.The effects of time after colonic injuries,complication,and the operation ways on the short-term recovery rate,secondary infection rate and mortality were observed.The effects of I stage operation and II stage operation on the prognosis of patients were observed also.Results Among 16 pati...
Objective To explore the treatments of colonic injuries complicating multi-organ or multi-locum injuries.Methods 16 cases with colonic injuries complicating multi-organ or multi-locum injuries were analyzed retrospectively.The effects of time after colonic injuries,complication,and the operation ways on the short-term recovery rate,secondary infection rate and mortality were observed.The effects of I stage operation and II stage operation on the prognosis of patients were observed also.Results Among 16 pati...
2011, 32(03)
Abstract:
Objective To investigate the pathogenesis,clinical manifestations,relevant examinations,treatments and prognosis of ischemic myocardial injury(IMI)with upper gastrointestinal bleeding(UGIB).Method We restropectively analyzed the clinical data of 113 cases of ischemic myocardial injury(IMI)with upper gastrointestinal bleeding(UGIB).Results 66 inpatients had no typical performance of myocardial injury(58.40%),97 inpatients was getting better and discharged from hospital(85.84%),16 inpatients died(14.16%).Conc...
Objective To investigate the pathogenesis,clinical manifestations,relevant examinations,treatments and prognosis of ischemic myocardial injury(IMI)with upper gastrointestinal bleeding(UGIB).Method We restropectively analyzed the clinical data of 113 cases of ischemic myocardial injury(IMI)with upper gastrointestinal bleeding(UGIB).Results 66 inpatients had no typical performance of myocardial injury(58.40%),97 inpatients was getting better and discharged from hospital(85.84%),16 inpatients died(14.16%).Conc...
2011, 32(03)
Abstract:
Objective To break the traditional teacher-oriented teaching methods,to find more suitable teaching methods for the professional,in order to mobilize the active learning enthusiasm of students,to enhance the ability of thinking,analyzing and solving problems.Methods Forty students in 2007 Clinical Oncology class were selected,and given the scene teaching of problem-based learning(PBL),and the standardized patient(SP)and teachers were enrolled to build teaching scene which can introduce the teaching content ...
Objective To break the traditional teacher-oriented teaching methods,to find more suitable teaching methods for the professional,in order to mobilize the active learning enthusiasm of students,to enhance the ability of thinking,analyzing and solving problems.Methods Forty students in 2007 Clinical Oncology class were selected,and given the scene teaching of problem-based learning(PBL),and the standardized patient(SP)and teachers were enrolled to build teaching scene which can introduce the teaching content ...
2011, 32(03)
Abstract:
Objective To study the specific identification of NeuN and glial fibrillary acidic protein(GFAP) antibody in spinal cords of adult monkeys and rats.Methods The spinal cords of monkeys and rats were harvested,and frozen sections from spinal cords were prepared.Then immunohistochemistry staining was performed to detect the specificity and distribution of NeuN and GFAP in neurons and astrocytes from the spinal cords of adult monkeys and rats.Results NeuN positive staining neurons were detected in the spinal co...
Objective To study the specific identification of NeuN and glial fibrillary acidic protein(GFAP) antibody in spinal cords of adult monkeys and rats.Methods The spinal cords of monkeys and rats were harvested,and frozen sections from spinal cords were prepared.Then immunohistochemistry staining was performed to detect the specificity and distribution of NeuN and GFAP in neurons and astrocytes from the spinal cords of adult monkeys and rats.Results NeuN positive staining neurons were detected in the spinal co...
