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RSS2011 Vol. 32, No. 07
2011, 32(07)
Abstract:
Objective To investigate the clinic features of chronic obstructive pulmonary disease(COPD)patients complicated with pulmonary embolism(PE)Method The clinical data of 42 COPD patients complicated with PE were retrospectively analyzed and compared with the data of 42 COPD patients.Results In the COPD with PE group,the incidence of chest pain,hemoptysis,fever,pleural effusion and asymmetric leg edema were obviously higher than that of only COPD group,and pulmonary artery pressure was significantly higher in t...
Objective To investigate the clinic features of chronic obstructive pulmonary disease(COPD)patients complicated with pulmonary embolism(PE)Method The clinical data of 42 COPD patients complicated with PE were retrospectively analyzed and compared with the data of 42 COPD patients.Results In the COPD with PE group,the incidence of chest pain,hemoptysis,fever,pleural effusion and asymmetric leg edema were obviously higher than that of only COPD group,and pulmonary artery pressure was significantly higher in t...
2011, 32(07)
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Objective To study the difference in the quantity expression of testicular tissues between patients with obstructive and non-obstructive azoospermia,and to explore the relationship between the relative quantity of FSHR and SCF expression and the spermiogenesis.Methods 36 testicular paraffin samples from 2007 to 2009,and 19 testicular samples from 2008 to 2010 with azoospermia were collected and divided into two groups: obstructive and non-obstructive azoospermia groups,according to the pathological results....
Objective To study the difference in the quantity expression of testicular tissues between patients with obstructive and non-obstructive azoospermia,and to explore the relationship between the relative quantity of FSHR and SCF expression and the spermiogenesis.Methods 36 testicular paraffin samples from 2007 to 2009,and 19 testicular samples from 2008 to 2010 with azoospermia were collected and divided into two groups: obstructive and non-obstructive azoospermia groups,according to the pathological results....
2011, 32(07)
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Objective To investigate the prevalence of depression and its risk factors in type 2 diabetics.Methods CES-D Scale was used to screen depression in 176 diebetes patients [male:81cases,female:95cases].Depression was defined when the depression index was more than 16.Meanwhile,the demographic data,metabolic data and diabetes-related health behaviors were also investigated.We counted out the prevalence of depression in diabetics,used Chi-square statistic to conduct univariate analysis and unconditional multiva...
Objective To investigate the prevalence of depression and its risk factors in type 2 diabetics.Methods CES-D Scale was used to screen depression in 176 diebetes patients [male:81cases,female:95cases].Depression was defined when the depression index was more than 16.Meanwhile,the demographic data,metabolic data and diabetes-related health behaviors were also investigated.We counted out the prevalence of depression in diabetics,used Chi-square statistic to conduct univariate analysis and unconditional multiva...
2011, 32(07)
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Objective To evaluate the efficacy and safety of Sanchinoside for diabetic peripheral neuropathy(DPN).Methods By using Cochrane systemic evaluation method,we retrieved related database and journals by computer retrieval and handiwork retrieval.The randomized controlled trials of clinical therapeutic studies on DPN treatment by Chinese medicine herb PNS were included and analyzed according to the criteria of the Cochrane Handbook.Meta analysis was used in this study.Results Seven RCTs involving 592 patients ...
Objective To evaluate the efficacy and safety of Sanchinoside for diabetic peripheral neuropathy(DPN).Methods By using Cochrane systemic evaluation method,we retrieved related database and journals by computer retrieval and handiwork retrieval.The randomized controlled trials of clinical therapeutic studies on DPN treatment by Chinese medicine herb PNS were included and analyzed according to the criteria of the Cochrane Handbook.Meta analysis was used in this study.Results Seven RCTs involving 592 patients ...
2011, 32(07)
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Objective To investigate the effect of acute alcoholism on the condition of patients with craniocerebral injury.Methods The clinical and laboratory data of 93 patients with craniocerebral injury treated in our hospital were analyzed retrospectively from Apr 2005 to Dec 2007.The patients were divided into two groups according to alcohol drinking or not.The general information and laboratory indexes on admission,complications and therapeutic effects of patients were compared between the two groups.Results Acu...
Objective To investigate the effect of acute alcoholism on the condition of patients with craniocerebral injury.Methods The clinical and laboratory data of 93 patients with craniocerebral injury treated in our hospital were analyzed retrospectively from Apr 2005 to Dec 2007.The patients were divided into two groups according to alcohol drinking or not.The general information and laboratory indexes on admission,complications and therapeutic effects of patients were compared between the two groups.Results Acu...
2011, 32(07)
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Objective To investigate the performance and value of low-field MRI in diagnosis of choroidal fissure cyst in brain.Method We retrospectively analyzed the low-field MRI findings of 13 choroidal fissure cyst in brain,and analyzed their correlation with clinical findings.Results 13 cases were cracked in the choroid,1 case occurred in the bilateral,7 cases occurred in the right side,5 cases occurred in the left side,the performance of the round,oval or spindle and smooth sharp boundary of the cerebrospinal flu...
Objective To investigate the performance and value of low-field MRI in diagnosis of choroidal fissure cyst in brain.Method We retrospectively analyzed the low-field MRI findings of 13 choroidal fissure cyst in brain,and analyzed their correlation with clinical findings.Results 13 cases were cracked in the choroid,1 case occurred in the bilateral,7 cases occurred in the right side,5 cases occurred in the left side,the performance of the round,oval or spindle and smooth sharp boundary of the cerebrospinal flu...
2011, 32(07)
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Objective To improve the detection rate,accuracy rate and the clinical value of ultrosonography in the diagnosis of peripheral neurilemmoma.Methods The sonographic features were analyzed retrospectively on twenty-four peripheral neurilemmoma lesions confirmed by surgical pathology.The sonographic characteristics and misdiagnostic analysis of peripheral neurilemmoma was summarized.Results The sonographic characteristics of peripheral neurilemmoma were oval-shaped and hypoechoic,the border were clear,the echo...
Objective To improve the detection rate,accuracy rate and the clinical value of ultrosonography in the diagnosis of peripheral neurilemmoma.Methods The sonographic features were analyzed retrospectively on twenty-four peripheral neurilemmoma lesions confirmed by surgical pathology.The sonographic characteristics and misdiagnostic analysis of peripheral neurilemmoma was summarized.Results The sonographic characteristics of peripheral neurilemmoma were oval-shaped and hypoechoic,the border were clear,the echo...
2011, 32(07)
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Objective To investigate the expression of tissue Transglutaminase(tTG)in renal tissue of patients with chronic renal diseases,so as to analyze the clinical significance of tTG.Methods Biopsy samples from 37 patients with chronic renal disease were divided into four groups according to their primary disease,including LN group(n=15),PSGN group(n=7),IgA N group(n=9)and Nephritis of anaphylactoid puerperal group(n=6).Meanwhile,control group was established with 5 normal renal tissues.Expression of tTG in renal...
Objective To investigate the expression of tissue Transglutaminase(tTG)in renal tissue of patients with chronic renal diseases,so as to analyze the clinical significance of tTG.Methods Biopsy samples from 37 patients with chronic renal disease were divided into four groups according to their primary disease,including LN group(n=15),PSGN group(n=7),IgA N group(n=9)and Nephritis of anaphylactoid puerperal group(n=6).Meanwhile,control group was established with 5 normal renal tissues.Expression of tTG in renal...
2011, 32(07)
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Objective To study the correlation of ankle-brachial index(ABI),brachial ankle pulse wave velocity(Ba-PWV)and the severity of acute coronary syndrome(ACS),so as to find out the value of ABI and Ba-PWV in prediciton of acute coronary syndrome.Methods We collected 220 patiens admitted in cardiology department who under went coronary artery angiography(CAG)from August 2007 to August 2008.The patients were divided into normal group and ACS group by the result of CAG.According to the number of suffered coronary ...
Objective To study the correlation of ankle-brachial index(ABI),brachial ankle pulse wave velocity(Ba-PWV)and the severity of acute coronary syndrome(ACS),so as to find out the value of ABI and Ba-PWV in prediciton of acute coronary syndrome.Methods We collected 220 patiens admitted in cardiology department who under went coronary artery angiography(CAG)from August 2007 to August 2008.The patients were divided into normal group and ACS group by the result of CAG.According to the number of suffered coronary ...
2011, 32(07)
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Objective To understand the situation of community health care in Kunming.residents' awareness of general practitioners,general practitioner services,the masses of the content and work,so as to provide the basis for the improvement of GP services requirements and training methods in future.Method The survey questionnaire was used to investigate 520 residents in Kunming with differente age,sex and occupation.Results Among the respondents,the percentage of awareness of diverse civilizational classes residents...
Objective To understand the situation of community health care in Kunming.residents' awareness of general practitioners,general practitioner services,the masses of the content and work,so as to provide the basis for the improvement of GP services requirements and training methods in future.Method The survey questionnaire was used to investigate 520 residents in Kunming with differente age,sex and occupation.Results Among the respondents,the percentage of awareness of diverse civilizational classes residents...
2011, 32(07)
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Objective To observe the therapeutic effect of peppermint oil for Demodex in human body surface.Methods 82 positive volunteers were divided into two groups.One as study group included 42 cases,another as control group included 40 cases.6.25% peppermint oil and 10% sulphur ointment were respectively used to treat the volunteers in two goups for 25 days in order to knowing the therapeutic effect of peppermint oil.Results The effective rate of the group of peppermint oil was 85.7%(36/42),and 42.5% of the group...
Objective To observe the therapeutic effect of peppermint oil for Demodex in human body surface.Methods 82 positive volunteers were divided into two groups.One as study group included 42 cases,another as control group included 40 cases.6.25% peppermint oil and 10% sulphur ointment were respectively used to treat the volunteers in two goups for 25 days in order to knowing the therapeutic effect of peppermint oil.Results The effective rate of the group of peppermint oil was 85.7%(36/42),and 42.5% of the group...
2011, 32(07)
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Objective To explore the clinical value of precise hepatectomy in liver surgery.Methods The clinical data of 64 patients with hepatobilillary diseases treated with hepatectomy in our hospital from 2009 to 2010 were retrospectively analyzed.The liver dysfunction and prognosis were compared between two different hepaqtectomy ways.Result There was a significant difference in the recovery of liver dysfunction and the incidence of complications between precise hepatectomy and pringle hepatectomy(P<0.05).Conclusi...
Objective To explore the clinical value of precise hepatectomy in liver surgery.Methods The clinical data of 64 patients with hepatobilillary diseases treated with hepatectomy in our hospital from 2009 to 2010 were retrospectively analyzed.The liver dysfunction and prognosis were compared between two different hepaqtectomy ways.Result There was a significant difference in the recovery of liver dysfunction and the incidence of complications between precise hepatectomy and pringle hepatectomy(P<0.05).Conclusi...
2011, 32(07)
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Objective To investigate the value of perioperative parenteral nutrition in postoperative rehabilitation of patients with colon cancer.Methods 129 colon cancer patients were recruited for this comparative study.69 patients received the program of perioperative parenteral nutrition support;meanwhile other 60 received conventional perioperative care.Results Patients in experimental group had a significantly shorter postoperative hospital stay,less medical cost,earlier first passage of flatus,less loss of body...
Objective To investigate the value of perioperative parenteral nutrition in postoperative rehabilitation of patients with colon cancer.Methods 129 colon cancer patients were recruited for this comparative study.69 patients received the program of perioperative parenteral nutrition support;meanwhile other 60 received conventional perioperative care.Results Patients in experimental group had a significantly shorter postoperative hospital stay,less medical cost,earlier first passage of flatus,less loss of body...
2011, 32(07)
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Objective To investigate the therapeutic effects of surgical treatment by approach of olecroanon osteotomy on intercondylar fracture of humerus in elderly patients and compared with expectant treatment.Methods We retrospectively analyzed the data of 34 elderly patients with intercondylar fracture of humerus from July 2006 to July 2009,including 17 male and 17 female patients,aged from 60 to 79,average 68.2.According to Riseborough classification,there were 6 patients with type I,11 patients with type II,12 ...
Objective To investigate the therapeutic effects of surgical treatment by approach of olecroanon osteotomy on intercondylar fracture of humerus in elderly patients and compared with expectant treatment.Methods We retrospectively analyzed the data of 34 elderly patients with intercondylar fracture of humerus from July 2006 to July 2009,including 17 male and 17 female patients,aged from 60 to 79,average 68.2.According to Riseborough classification,there were 6 patients with type I,11 patients with type II,12 ...
2011, 32(07)
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Objective To test the levels of platelet membrane glycoprotein PAC-1 and CD62P in elderly patients with type 2 diabetes mellitus(T2DM),impaired glucose tolerance(IGT)and impaired fasting glucose(IFG),and to investigate platelet activation under conditions of different blood glucose levels.Methods Thirty elderly patients with respective T2DM,IGT or IFG as well as normal controls(NC)were enrolled.The venous blood of patients in each group were collected for the examinations of the fasting plasma glucose(FPG),...
Objective To test the levels of platelet membrane glycoprotein PAC-1 and CD62P in elderly patients with type 2 diabetes mellitus(T2DM),impaired glucose tolerance(IGT)and impaired fasting glucose(IFG),and to investigate platelet activation under conditions of different blood glucose levels.Methods Thirty elderly patients with respective T2DM,IGT or IFG as well as normal controls(NC)were enrolled.The venous blood of patients in each group were collected for the examinations of the fasting plasma glucose(FPG),...
2011, 32(07)
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Objective To investigate the clinical efficacy and safety of ambroxol hydrochloride in preventing and treating neonatal respiratory distress syndrome(NRDS).Methods 91 premature infants were divided into two groups randomly.45 premature infants in control group were preformed with combined therapy.On this basis,46 cases in treatment group were given ambroxol hydrochloride,7.5 mg/kg,intervenous drop infusion,once every six hours,the course of treatment was 7 days.Results The incidence of NRDS in the treatment...
Objective To investigate the clinical efficacy and safety of ambroxol hydrochloride in preventing and treating neonatal respiratory distress syndrome(NRDS).Methods 91 premature infants were divided into two groups randomly.45 premature infants in control group were preformed with combined therapy.On this basis,46 cases in treatment group were given ambroxol hydrochloride,7.5 mg/kg,intervenous drop infusion,once every six hours,the course of treatment was 7 days.Results The incidence of NRDS in the treatment...
2011, 32(07)
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Objective To discuss the therapeutic effect of electrocautery to treat perennial allergic rhinitis.Methods Sixty-two cases with perennial allergic rhinitis were treated with bipolar electrocoagulation under the nasal endoscope,by fulgurating the pterygopalatine nerve,anterior ethmoidal nerve and the inferior turbinate simultaneously.Regular check was performed for each patient.The effects were analyzed after a follow-up of 2 years.Results Bipolar electrocoagulation for each case was done by one operation.Th...
Objective To discuss the therapeutic effect of electrocautery to treat perennial allergic rhinitis.Methods Sixty-two cases with perennial allergic rhinitis were treated with bipolar electrocoagulation under the nasal endoscope,by fulgurating the pterygopalatine nerve,anterior ethmoidal nerve and the inferior turbinate simultaneously.Regular check was performed for each patient.The effects were analyzed after a follow-up of 2 years.Results Bipolar electrocoagulation for each case was done by one operation.Th...
2011, 32(07)
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Objective To investigate the applying situation of pulmonary function test in the diagnosis of respiratory system disease in children.Methods We collected the clinical data of 1260 pediatric outpatients and 1 360 adult outpatients in our hospital from February 2010 to February 2011,and investigated and compared the ratio of pulmonary function test.Results 143 pediatric outpatients received pulmonary function test,including 64 with suspected asthma and 17 with confirmed asthma;673 adult outpatients received ...
Objective To investigate the applying situation of pulmonary function test in the diagnosis of respiratory system disease in children.Methods We collected the clinical data of 1260 pediatric outpatients and 1 360 adult outpatients in our hospital from February 2010 to February 2011,and investigated and compared the ratio of pulmonary function test.Results 143 pediatric outpatients received pulmonary function test,including 64 with suspected asthma and 17 with confirmed asthma;673 adult outpatients received ...
2011, 32(07)
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Objective To evaluate the clinical application of T-SPOT.TB(enzyme-linked immunospot assay in detection of Mycobacterium tuberculosis infection)in diagnosis of HIV/AIDS and TB patients.Methods We used the T-SPOT.TB to detect T cells with sectetion of IFN-r specific for M.tuberculosis antigents in blood samples from 60 HIV/AIDS patients suspected with tuberculosis,30 healthy persons and 20 patients with other diseases.Results Among 60 HIV/AIDS patients suspected with tuberculosis,35 cases were diagnosed with...
Objective To evaluate the clinical application of T-SPOT.TB(enzyme-linked immunospot assay in detection of Mycobacterium tuberculosis infection)in diagnosis of HIV/AIDS and TB patients.Methods We used the T-SPOT.TB to detect T cells with sectetion of IFN-r specific for M.tuberculosis antigents in blood samples from 60 HIV/AIDS patients suspected with tuberculosis,30 healthy persons and 20 patients with other diseases.Results Among 60 HIV/AIDS patients suspected with tuberculosis,35 cases were diagnosed with...
2011, 32(07)
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Objective To compare the nursing baccalaureate curriculum of nursing schools between China and Australia and to explove the evidence for.Method Literature comparing,statistic analysis and case study were conducted in this study.Results There were many differences were identified in curriculum goal,curriculum content and structure,and practical course.Conclusions Learning ideas from Australia,the setup of nursing baccalaureate curriculum in China should be more independent from clinical medical sciences,and ...
Objective To compare the nursing baccalaureate curriculum of nursing schools between China and Australia and to explove the evidence for.Method Literature comparing,statistic analysis and case study were conducted in this study.Results There were many differences were identified in curriculum goal,curriculum content and structure,and practical course.Conclusions Learning ideas from Australia,the setup of nursing baccalaureate curriculum in China should be more independent from clinical medical sciences,and ...
2011, 32(07)
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Objective To analyze the application of creative thinking(convergent thinking and divergent thinking)in bilingual teaching of preclinical Stomatology.Methods 75 students from Grade 2007 in Stomatology Faculty of KMU were randomly divided into 2 groups,one of which(Study Group)were taught the same content by creative-thinking bilingual methods and the other of which(Control Group)were taught by traditional teaching methods.During and after experiments,grades for everyone and questionnaires were recorded and ...
Objective To analyze the application of creative thinking(convergent thinking and divergent thinking)in bilingual teaching of preclinical Stomatology.Methods 75 students from Grade 2007 in Stomatology Faculty of KMU were randomly divided into 2 groups,one of which(Study Group)were taught the same content by creative-thinking bilingual methods and the other of which(Control Group)were taught by traditional teaching methods.During and after experiments,grades for everyone and questionnaires were recorded and ...
2011, 32(07)
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Objective To study medical university P.E.innovation and cultivation of students' lifelong P.E.consciousness or ability,and provide consultation for university to develop P.E.teaching innovation.Method The paper studied P.E.teaching innovation of Kunming medical university using experiment teaching method.Result After teaching innovation,there was significant difference in the physical quality,the P.E.interest between study group and control group(P<0.05).Conclusion University P.E.teaching innovation can im...
Objective To study medical university P.E.innovation and cultivation of students' lifelong P.E.consciousness or ability,and provide consultation for university to develop P.E.teaching innovation.Method The paper studied P.E.teaching innovation of Kunming medical university using experiment teaching method.Result After teaching innovation,there was significant difference in the physical quality,the P.E.interest between study group and control group(P<0.05).Conclusion University P.E.teaching innovation can im...
2011, 32(07)
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Objective To analyze the purpose,attitude and methods for bilingual teaching among freshman of School of Public Health by latent class analysis provide scientific evidence for bilingual education in future.Methods We cluster encountered the students from grade 1 and 2 in school of public health and conducted the survey with self-administered questionnaire.Administering the descriptive statistical analysis by SPSS,we carried out the latent class analysis using LatentGOLD software.Result Through the method of...
Objective To analyze the purpose,attitude and methods for bilingual teaching among freshman of School of Public Health by latent class analysis provide scientific evidence for bilingual education in future.Methods We cluster encountered the students from grade 1 and 2 in school of public health and conducted the survey with self-administered questionnaire.Administering the descriptive statistical analysis by SPSS,we carried out the latent class analysis using LatentGOLD software.Result Through the method of...
2011, 32(07)
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Objective To establish Pure Cerebral Concussion(PCC)and Multiple Cerebral Concussion(MCC)rats model and observe the changes of microglias(MIG)by cell count after injury,so as to understand the additive effect mechanisms of MCC.Methods The PCC or MCC model was duplicated by using a metallic pendulum-striker concussive device.96 female and male Sprague-Dawley rats were randomly divided into twelve groups:2 d,4 d,8 d,16 d,24 d groups and MCC 1 d,2 d,4 d,8 d,16 d,24 d groups,one control group was used,AND eight...
Objective To establish Pure Cerebral Concussion(PCC)and Multiple Cerebral Concussion(MCC)rats model and observe the changes of microglias(MIG)by cell count after injury,so as to understand the additive effect mechanisms of MCC.Methods The PCC or MCC model was duplicated by using a metallic pendulum-striker concussive device.96 female and male Sprague-Dawley rats were randomly divided into twelve groups:2 d,4 d,8 d,16 d,24 d groups and MCC 1 d,2 d,4 d,8 d,16 d,24 d groups,one control group was used,AND eight...
2011, 32(07)
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Objective To amplify and clone the C57BL/6J mouse titf-2 gene and express its protein in the mouse marrow mesenchymal cell.Methods The DNA encoding the C57BL/6J mouse titf-2 gene was amplified by PCR from the mouse liver tissue.The titf-2 gene was inserted into plasmid pMD18-T.After sequencing,The titf-2 gene was cloned into eukaryotic expression vector pBROAD3-mcs to construct the recombinant expression vector pBROAD3-titf-2 and it was used to transfect the mouse marrow mesenchymal cell.The expression prod...
Objective To amplify and clone the C57BL/6J mouse titf-2 gene and express its protein in the mouse marrow mesenchymal cell.Methods The DNA encoding the C57BL/6J mouse titf-2 gene was amplified by PCR from the mouse liver tissue.The titf-2 gene was inserted into plasmid pMD18-T.After sequencing,The titf-2 gene was cloned into eukaryotic expression vector pBROAD3-mcs to construct the recombinant expression vector pBROAD3-titf-2 and it was used to transfect the mouse marrow mesenchymal cell.The expression prod...
2011, 32(07)
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Objective To observe the effects of major components in Yunnan Baiyao on the expression of VEGF during the process of experimental rabbit fracture healing.Methods 100 New Zealand rabbits were randomly divided into 5 groups in this experiment.After the fracture model of both radius was established by operation,every medical gelatin sponge which contains number1,2,3,4,6 Yunnan Baiyao component was put into all left bone defect of radius separately as 1,2,3,4,6 Yunnan Baiyao group,and put an medical gelatin sp...
Objective To observe the effects of major components in Yunnan Baiyao on the expression of VEGF during the process of experimental rabbit fracture healing.Methods 100 New Zealand rabbits were randomly divided into 5 groups in this experiment.After the fracture model of both radius was established by operation,every medical gelatin sponge which contains number1,2,3,4,6 Yunnan Baiyao component was put into all left bone defect of radius separately as 1,2,3,4,6 Yunnan Baiyao group,and put an medical gelatin sp...
2011, 32(07)
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Objective To establish an improved,simplified and effective hepatocyte isolation technique in order to increase cell production and decrease the prime cost.Methods The improved simple perfusion system was used to isolate rat hepatocytes by a two-step perfusion with collagenase in situ.Hepatocytes were separated from the dissociative cells by low speed centrifugation(500 rpm,3 min,3 times),and discontinous gradient centrifugation method was applied to purify the hepatocytes.The hepatocyte yields and viabilit...
Objective To establish an improved,simplified and effective hepatocyte isolation technique in order to increase cell production and decrease the prime cost.Methods The improved simple perfusion system was used to isolate rat hepatocytes by a two-step perfusion with collagenase in situ.Hepatocytes were separated from the dissociative cells by low speed centrifugation(500 rpm,3 min,3 times),and discontinous gradient centrifugation method was applied to purify the hepatocytes.The hepatocyte yields and viabilit...
2011, 32(07)
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Objective To detect serum creatine kinase and hemoglobin level,and to study the correlation between those parameters and physical ability,state of fatigue,tissue damage and acclimation of the environment during training.Methods 45 young male cadets of a military university were recruited and divided randomly into training group and control group,cadets in the training group underwent a 8-week weight carrying running train in humid-heat environment.After training,cadets in both groups were performed running ...
Objective To detect serum creatine kinase and hemoglobin level,and to study the correlation between those parameters and physical ability,state of fatigue,tissue damage and acclimation of the environment during training.Methods 45 young male cadets of a military university were recruited and divided randomly into training group and control group,cadets in the training group underwent a 8-week weight carrying running train in humid-heat environment.After training,cadets in both groups were performed running ...
2011, 32(07)
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Objective To investigate the growth characteristics of human lung adenocarcinoma cell lines A549 in vitro.Methods A549 cell lines were cultured,and proliferative capacity was detected by immunohistochemistry staining of Brdu marker.On 1 day,18 days,22 days after culture,cell numbers were counted,cell vitality were tested by MTT and statistically analyzed.Results Mouse anti-human p63 protein,mouse anti-human cytokeratin 7,mouse anti-human thyroid transcription factor were positive staining by immunohistochem...
Objective To investigate the growth characteristics of human lung adenocarcinoma cell lines A549 in vitro.Methods A549 cell lines were cultured,and proliferative capacity was detected by immunohistochemistry staining of Brdu marker.On 1 day,18 days,22 days after culture,cell numbers were counted,cell vitality were tested by MTT and statistically analyzed.Results Mouse anti-human p63 protein,mouse anti-human cytokeratin 7,mouse anti-human thyroid transcription factor were positive staining by immunohistochem...
2011, 32(07)
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Objective To compare cell viability between Xuanwei lung adenocarcinoma cell lines xwlc-05 and cell lines A549 in vitro.Methods Xuanwei Lung adenocarcinoma cell lines xwlc-05 and cell lines A549 were cultured and morphological changes were observed.The number of adenocarcinoma cells and the cell viability were tested and statistically analyzed at 3 time points after culturing for 1 day,3 days and 5 days.Results The number of lung adenocarcinoma cell lines xwlc-05 and A549 gradually increased on 1 day,3 days...
Objective To compare cell viability between Xuanwei lung adenocarcinoma cell lines xwlc-05 and cell lines A549 in vitro.Methods Xuanwei Lung adenocarcinoma cell lines xwlc-05 and cell lines A549 were cultured and morphological changes were observed.The number of adenocarcinoma cells and the cell viability were tested and statistically analyzed at 3 time points after culturing for 1 day,3 days and 5 days.Results The number of lung adenocarcinoma cell lines xwlc-05 and A549 gradually increased on 1 day,3 days...
2011, 32(07)
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Objective To search new product and new idea for establishing DNA database at the grassroots level.Methods We tested 3977 blood samples by direct amplification Kit Goldeneye 20A-M and compared the success rate amont many types of blood samples.Meanwhile,we also explored the elution process of Whatman FTA card.Results The total checkout success rate of all blood samples by Goldeneye 20A-M kit was 99.4%.The checkout success rate was 100% for common blood filter paper,cotton bud blood sample,bandage blood samp...
Objective To search new product and new idea for establishing DNA database at the grassroots level.Methods We tested 3977 blood samples by direct amplification Kit Goldeneye 20A-M and compared the success rate amont many types of blood samples.Meanwhile,we also explored the elution process of Whatman FTA card.Results The total checkout success rate of all blood samples by Goldeneye 20A-M kit was 99.4%.The checkout success rate was 100% for common blood filter paper,cotton bud blood sample,bandage blood samp...
2011, 32(07)
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Objective To investigate the expression and its significance of myelin basic protein(MBP) in lung adenocarcinoma cells A549.Methods RPMI Medium 1640 with 10% fetal calf serum was used to culture lung adenocarcinoma cells A549,then the expression of MBP in lung adenocarcinoma cells A549 was detected by immunohistochemistry SP.Results Expression of MBP was mainly located in cytoplasm of lung adenocarcinoma cells A549,which exhibited a round shape,possibly as new cells.The positive rate of MBP positive cells w...
Objective To investigate the expression and its significance of myelin basic protein(MBP) in lung adenocarcinoma cells A549.Methods RPMI Medium 1640 with 10% fetal calf serum was used to culture lung adenocarcinoma cells A549,then the expression of MBP in lung adenocarcinoma cells A549 was detected by immunohistochemistry SP.Results Expression of MBP was mainly located in cytoplasm of lung adenocarcinoma cells A549,which exhibited a round shape,possibly as new cells.The positive rate of MBP positive cells w...
