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RSS2011 Vol. 32, No. 08
2011, 32(08)
Abstract:
Objective To discuss the potential role of C-type natriuretic peptide(CNP)in vascular restenosis prevention after aortic sac plasty by observating the influence of CNP on the intima hyperplasia.Methods Reconstructed plasmid pcDNA3.1/hCNP was transfected with polyethylenimine(PEI)by catheter method in iliac artery of rabbit model of atherosclerosis.The expression of pcDNA3.1/hCNP was confirmed with RT-PCR,immunohistochemistry and Western blot.The hyperplasia of intima was detected with elastic fibers stainin...
Objective To discuss the potential role of C-type natriuretic peptide(CNP)in vascular restenosis prevention after aortic sac plasty by observating the influence of CNP on the intima hyperplasia.Methods Reconstructed plasmid pcDNA3.1/hCNP was transfected with polyethylenimine(PEI)by catheter method in iliac artery of rabbit model of atherosclerosis.The expression of pcDNA3.1/hCNP was confirmed with RT-PCR,immunohistochemistry and Western blot.The hyperplasia of intima was detected with elastic fibers stainin...
2011, 32(08)
Abstract:
Objective Growth factors are used to accelerate osseous integrations.Usually,growth factors are destroyed easily and fixed difficultly in oral implantology area.The aim of this study was to prepare chitosans membrane with different molecular weight,and provide evidence for the application of chitosans in oral implants.Method Chitosans membranes and gelatin-chitosans membranes were prepared with chitosans with different molecular weight(3 000,100,000,200,000,450,000)by solvent evaporation method.Results Chit...
Objective Growth factors are used to accelerate osseous integrations.Usually,growth factors are destroyed easily and fixed difficultly in oral implantology area.The aim of this study was to prepare chitosans membrane with different molecular weight,and provide evidence for the application of chitosans in oral implants.Method Chitosans membranes and gelatin-chitosans membranes were prepared with chitosans with different molecular weight(3 000,100,000,200,000,450,000)by solvent evaporation method.Results Chit...
2011, 32(08)
Abstract:
Objective To establish a LC-MS method to measure the concentration of febuxostat in plasma and to investigate pharmacokinetic profile and absolute bioavailability of the drug in BALB/c mice.Methods Febuxostat was given intravenously to mice at 10 mg/kg or orally at 50 mg/kg,respectively.Blood samples were collected at various time following drug administration.Plasma concentration of febuxostat in mice was determined by LC-MS.Pharmacokinetics parameters were calculated by DAS software,and absolute bioavaila...
Objective To establish a LC-MS method to measure the concentration of febuxostat in plasma and to investigate pharmacokinetic profile and absolute bioavailability of the drug in BALB/c mice.Methods Febuxostat was given intravenously to mice at 10 mg/kg or orally at 50 mg/kg,respectively.Blood samples were collected at various time following drug administration.Plasma concentration of febuxostat in mice was determined by LC-MS.Pharmacokinetics parameters were calculated by DAS software,and absolute bioavaila...
2011, 32(08)
Abstract:
Objective To investigate the expressions and significance of ADAM10,EGFR and E-cadherin in hepatocellular carcinoma.Methods Immunohistochemistry and real time PCR were used to detect the expressions of ADAM10,EGFR and E-cadherin in 40 cases of hepatocellular carcinoma and analyze their relationshipship with clinic pathorelation.Results The expressions of ADAM10,EGFR and E-cadherin were significantly related to cancer epistom in portal vein,transplantation in liver,cell differentiation,size of cancer,and dis...
Objective To investigate the expressions and significance of ADAM10,EGFR and E-cadherin in hepatocellular carcinoma.Methods Immunohistochemistry and real time PCR were used to detect the expressions of ADAM10,EGFR and E-cadherin in 40 cases of hepatocellular carcinoma and analyze their relationshipship with clinic pathorelation.Results The expressions of ADAM10,EGFR and E-cadherin were significantly related to cancer epistom in portal vein,transplantation in liver,cell differentiation,size of cancer,and dis...
2011, 32(08)
Abstract:
Objective To compare the morphological changes between the Xuanwei lung cancer primary cells and established Xuanwei lung adenocarcinoma cell line XWLC-05.Methods Xuanwei lung adenocarcinoma cells and XWLC-05 cell line were cultured with 10% fetal bovine serum RPMI Medium 1640,and the growth characteristics of them were explored through observing the morphological changes and comparing the number of lung cancer cells at different time points.Results The primary lung cancer cells cultured in our laboratory g...
Objective To compare the morphological changes between the Xuanwei lung cancer primary cells and established Xuanwei lung adenocarcinoma cell line XWLC-05.Methods Xuanwei lung adenocarcinoma cells and XWLC-05 cell line were cultured with 10% fetal bovine serum RPMI Medium 1640,and the growth characteristics of them were explored through observing the morphological changes and comparing the number of lung cancer cells at different time points.Results The primary lung cancer cells cultured in our laboratory g...
2011, 32(08)
Abstract:
Objective To investigate the expression of APC and NeuN in the lung adenocarcinoma cell lines A549 and its significance.Methods Cell lines A549 were cultured and their morphological changes were observed.After culturing for 1 day and 5 days,expression of APC and NeuN in cells were detected by immunohistochemistry and the change of positive staining cells were counted.Results APC and NeuN expressed in human lung adenocarcinoma cell lines A549 in vitro.In the process of cell growth,the expression of APC reduc...
Objective To investigate the expression of APC and NeuN in the lung adenocarcinoma cell lines A549 and its significance.Methods Cell lines A549 were cultured and their morphological changes were observed.After culturing for 1 day and 5 days,expression of APC and NeuN in cells were detected by immunohistochemistry and the change of positive staining cells were counted.Results APC and NeuN expressed in human lung adenocarcinoma cell lines A549 in vitro.In the process of cell growth,the expression of APC reduc...
2011, 32(08)
Abstract:
Objective To study the expression and cell viability changes of GDNF in lung adenocarcinoma cell lines A549.Methods Lung adenocarcinoma cell lines A549 were cultured and morphological changes were observed.Cells were counted and cell viability were detected by MTT method after culturing for 1d and 5 d.timultaneously,GDNF positive cells were detected by immunohistochemistry and statistically analyzed.Results The cell number of lung adenocarcinoma cell lines A549 was significantly increased in 5 d group,compa...
Objective To study the expression and cell viability changes of GDNF in lung adenocarcinoma cell lines A549.Methods Lung adenocarcinoma cell lines A549 were cultured and morphological changes were observed.Cells were counted and cell viability were detected by MTT method after culturing for 1d and 5 d.timultaneously,GDNF positive cells were detected by immunohistochemistry and statistically analyzed.Results The cell number of lung adenocarcinoma cell lines A549 was significantly increased in 5 d group,compa...
2011, 32(08)
Abstract:
Objective To investigate the expression and its significance of nestin in human lung adenocarcinoma A549 cells.Methods RPMI Medium 1640 with 10% fetal serum was used to culture lung adenocarcinoma A549 cells,the expression of lung cancer markers and nestin was detected by immunohistochemisry.Results Staining of the mouse anti-human p63 protein,mouse anti-human cytokeratin 7,mouse anti-human thyroid transcription factor in four lung cancer markers were positive.Nestin was expressed in some lung cancer cells....
Objective To investigate the expression and its significance of nestin in human lung adenocarcinoma A549 cells.Methods RPMI Medium 1640 with 10% fetal serum was used to culture lung adenocarcinoma A549 cells,the expression of lung cancer markers and nestin was detected by immunohistochemisry.Results Staining of the mouse anti-human p63 protein,mouse anti-human cytokeratin 7,mouse anti-human thyroid transcription factor in four lung cancer markers were positive.Nestin was expressed in some lung cancer cells....
2011, 32(08)
Abstract:
Objective To investigate the expression of neurotrophic factors(GDNF,BDNF,NT3,NT4)in human lung adenocarcinoma cell lines A549 and explore the functional significance.Method After cell lines A549 were cultured and morphological changes were observed,the expression of neurotrophic factors GDNF,BDNF,NT3 and NT4 were detected by immunohistochemistry in cell lines A549 on 3 days.Result Neurotrophic factors GDNF,BDNF,NT3and NT4 were expressed in part of cell lines A549.Conclusion The neurotrophic factors GDNF,BD...
Objective To investigate the expression of neurotrophic factors(GDNF,BDNF,NT3,NT4)in human lung adenocarcinoma cell lines A549 and explore the functional significance.Method After cell lines A549 were cultured and morphological changes were observed,the expression of neurotrophic factors GDNF,BDNF,NT3 and NT4 were detected by immunohistochemistry in cell lines A549 on 3 days.Result Neurotrophic factors GDNF,BDNF,NT3and NT4 were expressed in part of cell lines A549.Conclusion The neurotrophic factors GDNF,BD...
2011, 32(08)
Abstract:
Objective To explore the clinical effect and evaluate the toxicity of transcatheterhepatic arterial chemoembolization(TACE)combined with gamma knife treatment on patients with primary hepatic carcinoma.Methods Total 96 patients with primary hepatic carcinoma were randomly divided into two groups:Group A-TACE combined with gamma knife(combined therapy group)and Group B-gamma knife only(control group),48 cases in each group.The short term effect,clinical benefit,survival time,and toxic side effects were copar...
Objective To explore the clinical effect and evaluate the toxicity of transcatheterhepatic arterial chemoembolization(TACE)combined with gamma knife treatment on patients with primary hepatic carcinoma.Methods Total 96 patients with primary hepatic carcinoma were randomly divided into two groups:Group A-TACE combined with gamma knife(combined therapy group)and Group B-gamma knife only(control group),48 cases in each group.The short term effect,clinical benefit,survival time,and toxic side effects were copar...
2011, 32(08)
Abstract:
Objective To investigate the value of high-frequency color Doppler ultrasound in diagnosis of knee joint's diseases.Method 28 knee joints of 14 patients were examined with high-frequency color doppler ultrasound to observe the synovial membrane,joint capsule effusion,cartilage and blood flow.Results The symptoms of arthritis including synovitis,joint capsule effusion and(or not)cartilage destruction were detected in 23 knee joints in 14 patients.No symptoms of arthritis were found in 5 knee joints.Conclusio...
Objective To investigate the value of high-frequency color Doppler ultrasound in diagnosis of knee joint's diseases.Method 28 knee joints of 14 patients were examined with high-frequency color doppler ultrasound to observe the synovial membrane,joint capsule effusion,cartilage and blood flow.Results The symptoms of arthritis including synovitis,joint capsule effusion and(or not)cartilage destruction were detected in 23 knee joints in 14 patients.No symptoms of arthritis were found in 5 knee joints.Conclusio...
2011, 32(08)
Abstract:
Objective To observe the effect of budesonide suspension for inhalation in the treatment of children with cough variant asthma(CVA).Methods 30 children with CVA were treated for 7 weeks with inhalation of budesonide(0.5 mg,Bid) and were followed up for 1,3,5 and 7 weeks after the treatment.The daytime and nocturnal cough score means were recorded.Results 26(86.7%)children's symptoms with CVA were controlled and 4(13.3%)children's symptoms were part of controlled after the treatment.The daytime and nocturnal...
Objective To observe the effect of budesonide suspension for inhalation in the treatment of children with cough variant asthma(CVA).Methods 30 children with CVA were treated for 7 weeks with inhalation of budesonide(0.5 mg,Bid) and were followed up for 1,3,5 and 7 weeks after the treatment.The daytime and nocturnal cough score means were recorded.Results 26(86.7%)children's symptoms with CVA were controlled and 4(13.3%)children's symptoms were part of controlled after the treatment.The daytime and nocturnal...
2011, 32(08)
Abstract:
Objective To observe the expressional changes of proNGF apoptosis pro-signal molecules in human brain gliomas and its clinical meanings.Methods RT-PCR(Reverse transcription poly-merase chain reaction)was used to detect the expressional changes of proNGF,Sortilin and P75 in human brain gliomas,and 10 cases of normal tissue around the gliomas were used as the control.Results All the factors were detected in gliomas,and lowly expressed in normal tissues,and there was statistically significant difference betwee...
Objective To observe the expressional changes of proNGF apoptosis pro-signal molecules in human brain gliomas and its clinical meanings.Methods RT-PCR(Reverse transcription poly-merase chain reaction)was used to detect the expressional changes of proNGF,Sortilin and P75 in human brain gliomas,and 10 cases of normal tissue around the gliomas were used as the control.Results All the factors were detected in gliomas,and lowly expressed in normal tissues,and there was statistically significant difference betwee...
2011, 32(08)
Abstract:
Objective To evaluate the clinical effect of particle repositioning maneuver on benign paroxysmal positional vertigo(BPPV).Method Totally 43 cases of BPPV were treated by means of different particle repositioning maneuveres according to the affected canal.Results One week after the last treatment for 43 patients of BPPV,32 cases were cured;9 cases had effects.The totally effective rate was 95.3%(41/43).Conclusion Particle repositioning maneuver is a safe,easy,cheap and effective treatment method for benign ...
Objective To evaluate the clinical effect of particle repositioning maneuver on benign paroxysmal positional vertigo(BPPV).Method Totally 43 cases of BPPV were treated by means of different particle repositioning maneuveres according to the affected canal.Results One week after the last treatment for 43 patients of BPPV,32 cases were cured;9 cases had effects.The totally effective rate was 95.3%(41/43).Conclusion Particle repositioning maneuver is a safe,easy,cheap and effective treatment method for benign ...
2011, 32(08)
Abstract:
Objective To implement a normalize pain education program for trauma patients by establishing a pain-nursing group,make them active cooperate with analgesic treatment,effective control the pain during perioperative period and make these patients feel more comfortable during this period.Methods All the patients received in June,2009 were divided into two groups randomly:observe group and control group.Patients in both groups were given normal analgesia treatment.Patients in observe group were instructed with...
Objective To implement a normalize pain education program for trauma patients by establishing a pain-nursing group,make them active cooperate with analgesic treatment,effective control the pain during perioperative period and make these patients feel more comfortable during this period.Methods All the patients received in June,2009 were divided into two groups randomly:observe group and control group.Patients in both groups were given normal analgesia treatment.Patients in observe group were instructed with...
2011, 32(08)
Abstract:
Objective To investigate the effective and toxicity of cisplatin concurrent chemotherapy and radiotherapy in local advanced cervical Cancer.Methods The cisplatin given at a dose of 30 mg(m2.w)for 6 cycles in 32 patients.External radiotherapy given at a dose 30 Gy firstly and then were irradiated for another 20 Gy under the condition of A point shielded plus intracavitary irradiation for 6 Gy twice a week to a total dose 36 Gy for A point.Results The effective rate and clinical benefits rate were both 94%.Th...
Objective To investigate the effective and toxicity of cisplatin concurrent chemotherapy and radiotherapy in local advanced cervical Cancer.Methods The cisplatin given at a dose of 30 mg(m2.w)for 6 cycles in 32 patients.External radiotherapy given at a dose 30 Gy firstly and then were irradiated for another 20 Gy under the condition of A point shielded plus intracavitary irradiation for 6 Gy twice a week to a total dose 36 Gy for A point.Results The effective rate and clinical benefits rate were both 94%.Th...
2011, 32(08)
Abstract:
Objective To study the characteristics of medicolegal investigation cases about intelligence defect caused by craniocerebral injury resulted from traffic accidents,and to discuss how to evaluate the intelligence defect through clinical data such as detection report of IQ and forensic examinaiton.Method We collected 68 cases of intelligence defect caused by craniocerebral injury after traffic accidents,and analyzed the difference among individual character,injury condition,grandiosity,camouflage and expertis...
Objective To study the characteristics of medicolegal investigation cases about intelligence defect caused by craniocerebral injury resulted from traffic accidents,and to discuss how to evaluate the intelligence defect through clinical data such as detection report of IQ and forensic examinaiton.Method We collected 68 cases of intelligence defect caused by craniocerebral injury after traffic accidents,and analyzed the difference among individual character,injury condition,grandiosity,camouflage and expertis...
2011, 32(08)
Abstract:
Objective To investigate the application value of the throat foreign body removal with forcep under the video laryngoscope of straight pipe.Method We retrospectively analyzed the clinical data of 826 patients with confirmed or suspected throat foreign bodies who received removal surgery throat foreign bodies under storz video laryngoscope of straight pipe through the mouth.Results Among 826 patients,throat foreign bodies were found in 802 patients and and successfully removed under the video laryngoscope of...
Objective To investigate the application value of the throat foreign body removal with forcep under the video laryngoscope of straight pipe.Method We retrospectively analyzed the clinical data of 826 patients with confirmed or suspected throat foreign bodies who received removal surgery throat foreign bodies under storz video laryngoscope of straight pipe through the mouth.Results Among 826 patients,throat foreign bodies were found in 802 patients and and successfully removed under the video laryngoscope of...
2011, 32(08)
Abstract:
Objective To explore the correlation of life quality of patients with liver cancer and their coping styles,and to provide information for care strategy.Method EROTCQLQ-C30 and MCMQ were used to investigate the life quality and coping styles of 30 liver cancer patients and 30 healthy controls.Results The scores of life quality of the cancer patients were significantly lower than the healthy controls.The most frequently adopted coping styles by the cancer patients were confrontation,fighting,avoidance,resigna...
Objective To explore the correlation of life quality of patients with liver cancer and their coping styles,and to provide information for care strategy.Method EROTCQLQ-C30 and MCMQ were used to investigate the life quality and coping styles of 30 liver cancer patients and 30 healthy controls.Results The scores of life quality of the cancer patients were significantly lower than the healthy controls.The most frequently adopted coping styles by the cancer patients were confrontation,fighting,avoidance,resigna...
2011, 32(08)
Abstract:
Objective To explore an effective measures to reduce adverse reactions in blood transfusion.Methords The reports with adverse reactions in blood transfusion in our hospital from 2006 to 2007 were analyzed retrospectively,and the adverse reactions caused by transfusion of blood products were analyzed.Results There were 60 of 26 302 reports with adverse reactions,about 0.23% and the rate of component transfusion was 94.1%.Conclusion Component transfusion is an effective measures to reduce adverse reactions in...
Objective To explore an effective measures to reduce adverse reactions in blood transfusion.Methords The reports with adverse reactions in blood transfusion in our hospital from 2006 to 2007 were analyzed retrospectively,and the adverse reactions caused by transfusion of blood products were analyzed.Results There were 60 of 26 302 reports with adverse reactions,about 0.23% and the rate of component transfusion was 94.1%.Conclusion Component transfusion is an effective measures to reduce adverse reactions in...
2011, 32(08)
Abstract:
Objective To discuss early diagnosis and treatment of tethered cord syndrome(TCS)by analyzing the clinical data of 46 cases.Method Examinations in nervous system,urodynamic study and MRI were conducted in all cases.The surgical release of TCS was performed in all cases and the therapeutic effectiveness after operation was graded according to the physical improvement of the patients:excellent,effective and ineffective.Results The degree of untethering achieved at surgery for tethered cord syndrome was assess...
Objective To discuss early diagnosis and treatment of tethered cord syndrome(TCS)by analyzing the clinical data of 46 cases.Method Examinations in nervous system,urodynamic study and MRI were conducted in all cases.The surgical release of TCS was performed in all cases and the therapeutic effectiveness after operation was graded according to the physical improvement of the patients:excellent,effective and ineffective.Results The degree of untethering achieved at surgery for tethered cord syndrome was assess...
2011, 32(08)
Abstract:
Objective To observe the clinical efficacy of Acanthopanax senticosus injection in the treatment of insomnia.Methods 60 insomnia cases were randomly divided into two groups:treatment group and control group(30 cases in each group).Patients in the treatment group were given A.senticosus injection while thoese in the control group were given Glu injection.The curative effect,score of Pittsburgh Sleep Quality Index(PSQI),Athens sleep quality score and adverse reactions were compared between the two groups.Resu...
Objective To observe the clinical efficacy of Acanthopanax senticosus injection in the treatment of insomnia.Methods 60 insomnia cases were randomly divided into two groups:treatment group and control group(30 cases in each group).Patients in the treatment group were given A.senticosus injection while thoese in the control group were given Glu injection.The curative effect,score of Pittsburgh Sleep Quality Index(PSQI),Athens sleep quality score and adverse reactions were compared between the two groups.Resu...
2011, 32(08)
Abstract:
Objective To evaluate the efficacy and safety of the single and double injection techniques for axmary brachial plexus block.Methods The selected 100 patients who were operated on the antebrachium and hand were randomly divided into two groups:S group(single injection)and D group(double injection),and 50 cases in each groups.In S group,axmary brachial plexus block was performed respectively with 7-gauge needle,and in D group,axmary brachial plexus block over the arillary artery and below the arillary artery...
Objective To evaluate the efficacy and safety of the single and double injection techniques for axmary brachial plexus block.Methods The selected 100 patients who were operated on the antebrachium and hand were randomly divided into two groups:S group(single injection)and D group(double injection),and 50 cases in each groups.In S group,axmary brachial plexus block was performed respectively with 7-gauge needle,and in D group,axmary brachial plexus block over the arillary artery and below the arillary artery...
