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RSS2011 Vol. 32, No. 09
2011, 32(09)
Abstract:
Objective To screen antitumor activity of the extracts from Panax Notoginseng in vitro and analyze components of the extracts.Methods The modified MTT method was chosen to study the extracts' anticancer activity on four kinds of human cancer cells in vitro.The chemical components were separated and identified by gas chromatography-mass spectrometry(GC-MS),and their relative contents were determined by normalization method of area.Results The half inhibitory concentration(IC50)value of the Panax Notoginseng ...
Objective To screen antitumor activity of the extracts from Panax Notoginseng in vitro and analyze components of the extracts.Methods The modified MTT method was chosen to study the extracts' anticancer activity on four kinds of human cancer cells in vitro.The chemical components were separated and identified by gas chromatography-mass spectrometry(GC-MS),and their relative contents were determined by normalization method of area.Results The half inhibitory concentration(IC50)value of the Panax Notoginseng ...
2011, 32(09)
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Objective To establish the traumatic brain injury(TBI)model in canis,and monitor the variations of topical biochemical substances by intracranial microdialysis.Methods Nine healthy adult male mongrel canines were randonmized into three groups:mild TBI group(n=3),severe TBI group(n=3),and control group(n=3).The model was established by the method of free falling body extradural hitting.The extracellular fluid(ECF)was collected by using the intracranial microdialysis.Then the content variations of Glucose,Lac...
Objective To establish the traumatic brain injury(TBI)model in canis,and monitor the variations of topical biochemical substances by intracranial microdialysis.Methods Nine healthy adult male mongrel canines were randonmized into three groups:mild TBI group(n=3),severe TBI group(n=3),and control group(n=3).The model was established by the method of free falling body extradural hitting.The extracellular fluid(ECF)was collected by using the intracranial microdialysis.Then the content variations of Glucose,Lac...
2011, 32(09)
Abstract:
Objective To explore the inducing effects of RA on the apotosis of primary cultured craniopharyngiomic cells.Methods Caniopharyngiomic cells were pimary cultured from fresh surgical samples,and then were treated with 20 mol RA for east 24 hours.Then these cells were collected for apoptotic tests which included Annexin V,DAPI staining and DNA ladder.Results RA treated primary cells presented higher level of Annexin V than untreated cells.Meanwhile DAPI staining indicated RA incubation leaded to pyknosis and ...
Objective To explore the inducing effects of RA on the apotosis of primary cultured craniopharyngiomic cells.Methods Caniopharyngiomic cells were pimary cultured from fresh surgical samples,and then were treated with 20 mol RA for east 24 hours.Then these cells were collected for apoptotic tests which included Annexin V,DAPI staining and DNA ladder.Results RA treated primary cells presented higher level of Annexin V than untreated cells.Meanwhile DAPI staining indicated RA incubation leaded to pyknosis and ...
2011, 32(09)
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Objective To observe the role of mitogen-activated protein kinases(MAPKs)in protection of exogenous hydrogen sulfide(H2S)against lung injury induced by ischemia-reperfusion(IR)of hind limbs in rats.Methods A rat model of limb ischemia was made by tourniquets placed on both hind limbs at a site proximal to trochanter major and the lung injury was induced following reperfusion.Adult male Spraguce-Dawley(SD)rats were randomly divided into four groups(n=8 per group):Control,Control+NaHS,IR and IR+ NaHS.The rats...
Objective To observe the role of mitogen-activated protein kinases(MAPKs)in protection of exogenous hydrogen sulfide(H2S)against lung injury induced by ischemia-reperfusion(IR)of hind limbs in rats.Methods A rat model of limb ischemia was made by tourniquets placed on both hind limbs at a site proximal to trochanter major and the lung injury was induced following reperfusion.Adult male Spraguce-Dawley(SD)rats were randomly divided into four groups(n=8 per group):Control,Control+NaHS,IR and IR+ NaHS.The rats...
2011, 32(09)
Abstract:
Objective To detect BDNF expression in the central nervous system(hippocampus and brainstem) of tree shrews.Method RT-PCR was used to detect BDNF expression in the central nervous system(hippocampus and brainstem) of tree shrews from embryo to adult.Results BDNF expressed in hippocampus and brainstem of tree shrews from embryo to adult.The expression level of BDNF mRNA in adult was significantly higher than that of embryo and newborn tree shrews(P<0.05).Conclusion The expression level of BDNF is still high ...
Objective To detect BDNF expression in the central nervous system(hippocampus and brainstem) of tree shrews.Method RT-PCR was used to detect BDNF expression in the central nervous system(hippocampus and brainstem) of tree shrews from embryo to adult.Results BDNF expressed in hippocampus and brainstem of tree shrews from embryo to adult.The expression level of BDNF mRNA in adult was significantly higher than that of embryo and newborn tree shrews(P<0.05).Conclusion The expression level of BDNF is still high ...
2011, 32(09)
Abstract:
Objective To summarize the experiences in the treatment of non-small cell lung cancer with full video-assisted thoracoscopic lobectomy.Methods 54 patients with non-small cell lung cancer were treated with complete thoracoscopic lobectomy and lymph node dissection.All patients were confirmed by preoperative or postoperative pathological examination.Results The tumors of 54 patients were completely resected,and postoperative pathology report showed no lung cancer cells in the resection margin.There were no pe...
Objective To summarize the experiences in the treatment of non-small cell lung cancer with full video-assisted thoracoscopic lobectomy.Methods 54 patients with non-small cell lung cancer were treated with complete thoracoscopic lobectomy and lymph node dissection.All patients were confirmed by preoperative or postoperative pathological examination.Results The tumors of 54 patients were completely resected,and postoperative pathology report showed no lung cancer cells in the resection margin.There were no pe...
2011, 32(09)
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Objective To investigate the effects of combination therapy of probucol,atorvastatin calcium and aspirin drugs(PAS)on patients with atherosclerosis.Method 120 patients with ischemic cerebrvascular disease and carotid arteriosclerosis were randomly divided into three groups:PAS group,AS group and A group,40 patients in each group.Patients in the PAS group were treated with probucol(0.5 g/d),atorvastatin calcium(20 mg/d)and aspirin(100 mg/d),patients in AS group were treated with atorvastatin calcium(20 mg/d)...
Objective To investigate the effects of combination therapy of probucol,atorvastatin calcium and aspirin drugs(PAS)on patients with atherosclerosis.Method 120 patients with ischemic cerebrvascular disease and carotid arteriosclerosis were randomly divided into three groups:PAS group,AS group and A group,40 patients in each group.Patients in the PAS group were treated with probucol(0.5 g/d),atorvastatin calcium(20 mg/d)and aspirin(100 mg/d),patients in AS group were treated with atorvastatin calcium(20 mg/d)...
2011, 32(09)
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Objective To investigate the situation about women's knowledge of breast cancer in Lijiang city and the influencing factors.Method 973 women were attended in this study using questionnaire.Results Only 363 women(37.3%)know nothing about breast cancer.Logistic model displayed that compared with Naxi ethnicity,Bai ethnic had more knowledge of breast cancer(OR=1.85,P=0.017)while there was no statistically significant difference between Han and Naxi ethnicity.There was also no statistically significant differen...
Objective To investigate the situation about women's knowledge of breast cancer in Lijiang city and the influencing factors.Method 973 women were attended in this study using questionnaire.Results Only 363 women(37.3%)know nothing about breast cancer.Logistic model displayed that compared with Naxi ethnicity,Bai ethnic had more knowledge of breast cancer(OR=1.85,P=0.017)while there was no statistically significant difference between Han and Naxi ethnicity.There was also no statistically significant differen...
2011, 32(09)
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Objective To explore the value of transvaginal ultrasonic(TVUS)diagnosis in salpingocyesis.Method The ultrasonic features of 202 patients with salpingocyesis were reviewed and analyzed.Results TVUS could be used to diagnose salpingocyesis immediately and accurately,with the accuracy up to 97.5%.Salpingocyesis can be divided into scrotum pregnancy,bag and drifted type,according to the TVUS features.Conclusion TVUS plays a crucial role in diagnosis of salpingocyesis in clinic.
Objective To explore the value of transvaginal ultrasonic(TVUS)diagnosis in salpingocyesis.Method The ultrasonic features of 202 patients with salpingocyesis were reviewed and analyzed.Results TVUS could be used to diagnose salpingocyesis immediately and accurately,with the accuracy up to 97.5%.Salpingocyesis can be divided into scrotum pregnancy,bag and drifted type,according to the TVUS features.Conclusion TVUS plays a crucial role in diagnosis of salpingocyesis in clinic.
2011, 32(09)
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Objective To evaluate the clinical application value of ECG modulated mA in CT coronary artery imaging.Method 58 patients were given coronary artery CTA with ECG modulated mA,and the image quality and radiation dose were analyzed.Result All images reached the standard of diagnosis.Conclusion 64-MSCT in coronary artery imaging with ECG modulated mA can reduce the radiation dose,and in the same time acquire the images up to standard of diagnosis.
Objective To evaluate the clinical application value of ECG modulated mA in CT coronary artery imaging.Method 58 patients were given coronary artery CTA with ECG modulated mA,and the image quality and radiation dose were analyzed.Result All images reached the standard of diagnosis.Conclusion 64-MSCT in coronary artery imaging with ECG modulated mA can reduce the radiation dose,and in the same time acquire the images up to standard of diagnosis.
2011, 32(09)
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Objective To observe Kangai injection combined with chemotherapy for advanced colorectal cancer patients to improve quality of life and to reduce the role of toxicity.Methods 60 cases of colorectal cancer patients were randomly divided into 2 groups:control group and treatment group,30 patients in each group.Patients in control group were treated with chemotherapy,and patients in treatment group were treated with chemotherapy and Kangai injection.14 days were as a course of treatment,and after 4 courses,the...
Objective To observe Kangai injection combined with chemotherapy for advanced colorectal cancer patients to improve quality of life and to reduce the role of toxicity.Methods 60 cases of colorectal cancer patients were randomly divided into 2 groups:control group and treatment group,30 patients in each group.Patients in control group were treated with chemotherapy,and patients in treatment group were treated with chemotherapy and Kangai injection.14 days were as a course of treatment,and after 4 courses,the...
Treatment of Pediatric Inguinal Hernia with Small Incision Surgery by Abdominal Pattern in 500 Cases
2011, 32(09)
Abstract:
Objective To evaluate the clinical effect of small incision surgery on pediatric inguinal henia.Method The clinical data of 200 cases with pediatric henia treated by traditional surgery and 500 cases by small incision surgery were analyzed retrospectively,including surgery time,bleeding volumn during surgery,locomotor activity time after surgery and related complication.Result Compared with traditional surgery,surgery time and postoperative locomotor activity time were shortened in small incision surgery gr...
Objective To evaluate the clinical effect of small incision surgery on pediatric inguinal henia.Method The clinical data of 200 cases with pediatric henia treated by traditional surgery and 500 cases by small incision surgery were analyzed retrospectively,including surgery time,bleeding volumn during surgery,locomotor activity time after surgery and related complication.Result Compared with traditional surgery,surgery time and postoperative locomotor activity time were shortened in small incision surgery gr...
2011, 32(09)
Abstract:
Objective To explore the effect of minimally invasive drainage and urokinase injection on chronic subdural hematoma.Methods 118 patients have been treated with needle puncturing into hematoma since 2003.Among them,52 patients were only drained after puncture,since 2007,other 66 patients were injected urokinase into hematoma for one to third times after puncture.Results The average time of hematoma complete drainage for 66 patients who accepted urokinase injection into hematoma was 4.1 days,and no recurrence...
Objective To explore the effect of minimally invasive drainage and urokinase injection on chronic subdural hematoma.Methods 118 patients have been treated with needle puncturing into hematoma since 2003.Among them,52 patients were only drained after puncture,since 2007,other 66 patients were injected urokinase into hematoma for one to third times after puncture.Results The average time of hematoma complete drainage for 66 patients who accepted urokinase injection into hematoma was 4.1 days,and no recurrence...
2011, 32(09)
Abstract:
Objective To observe the effects of exercise prescription on upper limb motor function and functional activity in stroke patients with hemiplegia.Methods 120 stroke patients with upper limb dysfunction were randomly divided into treatment group and control group,with 60 cases in each group.Patients in the treatment group were given exercise prescription and exercised according to sports prescription,patients in the control group exercised casually.The clinic effects were observed and compared between the tw...
Objective To observe the effects of exercise prescription on upper limb motor function and functional activity in stroke patients with hemiplegia.Methods 120 stroke patients with upper limb dysfunction were randomly divided into treatment group and control group,with 60 cases in each group.Patients in the treatment group were given exercise prescription and exercised according to sports prescription,patients in the control group exercised casually.The clinic effects were observed and compared between the tw...
2011, 32(09)
Abstract:
Objective To explore the application of absorbable internal fixation materials SR-P(L/DL)LA(70/30)in the jaw fractures.Method From July 2008 to August 2010,19 patients with maxillofacial fractures patients were treated by using absorbable internal fixation materials for fixation.Results Most cases of facial fracture gained satisfactory effects.All the incisions closed up in stage I.Ideal occlusion and stable fixed were gained in most patients,except for one plate fixed in mandibular body appeared disjunctio...
Objective To explore the application of absorbable internal fixation materials SR-P(L/DL)LA(70/30)in the jaw fractures.Method From July 2008 to August 2010,19 patients with maxillofacial fractures patients were treated by using absorbable internal fixation materials for fixation.Results Most cases of facial fracture gained satisfactory effects.All the incisions closed up in stage I.Ideal occlusion and stable fixed were gained in most patients,except for one plate fixed in mandibular body appeared disjunctio...
2011, 32(09)
Abstract:
Objective Primary hepatocellular carcinoma treatment and prognosis and occur at different stages of development are very relevant,early diagnosis and treatment of small hepatocellular carcinoma after 5-year survival rate was significantly higher than in advanced liver cancer,the probability for liver cancer higher in patients with close monitoring of the population has important implications.Methods We collected 360 middle-aged patients with chronic liver disease cases,and classified them as high risk of li...
Objective Primary hepatocellular carcinoma treatment and prognosis and occur at different stages of development are very relevant,early diagnosis and treatment of small hepatocellular carcinoma after 5-year survival rate was significantly higher than in advanced liver cancer,the probability for liver cancer higher in patients with close monitoring of the population has important implications.Methods We collected 360 middle-aged patients with chronic liver disease cases,and classified them as high risk of li...
2011, 32(09)
Abstract:
Objective To evaluate the effectiveness of intraaortic balloon counterpulsation(IABP)in the treatment of patients with acute coronary syndrome.Method The clinical data of 38 patients with acute coronary syndrome who received IABP to reconstruct blood vessels were retrospectively analyzed,and the clinical effect was evaluated.Results There were serious left cardiac dysfunction coronary multivessel disease and other risk factors in 38 cases.33 patients received routine percutaneous coronary intervention(pci),...
Objective To evaluate the effectiveness of intraaortic balloon counterpulsation(IABP)in the treatment of patients with acute coronary syndrome.Method The clinical data of 38 patients with acute coronary syndrome who received IABP to reconstruct blood vessels were retrospectively analyzed,and the clinical effect was evaluated.Results There were serious left cardiac dysfunction coronary multivessel disease and other risk factors in 38 cases.33 patients received routine percutaneous coronary intervention(pci),...
2011, 32(09)
Abstract:
Objective To investigate the value of transvaginal color Doppler ultrasound in diagnosis of congenital uterine malformation.Method The clinical data of patients with congenital uterine malformation in our hospital from 2009 January to 2011 June were retrospectively analyzed.Result 208 cases of uterine malformation were found,including 135 cases of septate uterus,31 cases of double uterus,bicornuate uterus in 9 cases,3 cases of residual slots,unicornuate uterus in 2 cases,congenital absence of child congenit...
Objective To investigate the value of transvaginal color Doppler ultrasound in diagnosis of congenital uterine malformation.Method The clinical data of patients with congenital uterine malformation in our hospital from 2009 January to 2011 June were retrospectively analyzed.Result 208 cases of uterine malformation were found,including 135 cases of septate uterus,31 cases of double uterus,bicornuate uterus in 9 cases,3 cases of residual slots,unicornuate uterus in 2 cases,congenital absence of child congenit...
2011, 32(09)
Abstract:
Objective To discuss the difference in conservative salpingian ectopic pregnancy between single-port transumbilical laparoscope and three-port laparoscope.Methods Patients were divided into two groups:32 patients were operated by single-port transumbilical laparoscope,and 55 patients were operated by three-port laparoscope.The difference in operation time,quantity of bleeding,pain of incision,passage of gas by anus and taking food time were compared between the two groups.Results The operation time by singl...
Objective To discuss the difference in conservative salpingian ectopic pregnancy between single-port transumbilical laparoscope and three-port laparoscope.Methods Patients were divided into two groups:32 patients were operated by single-port transumbilical laparoscope,and 55 patients were operated by three-port laparoscope.The difference in operation time,quantity of bleeding,pain of incision,passage of gas by anus and taking food time were compared between the two groups.Results The operation time by singl...
2011, 32(09)
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Objective To analyze the effects of the guideline of community-aquired pneumonia on the achievement ratio of CAP therapy.Methods The data of 417 community-aquired pneumonia patients was analyzed retrospectively.The effect of choosing the first dose antibiotics and CAP guideline on the achievement ratio of CAP therapy was explored.Results(1)The percentage of patients choosing the first dose antibiotic and following Chinese CAP guideline was 68.8%.There was no significant difference in the percentage in five ...
Objective To analyze the effects of the guideline of community-aquired pneumonia on the achievement ratio of CAP therapy.Methods The data of 417 community-aquired pneumonia patients was analyzed retrospectively.The effect of choosing the first dose antibiotics and CAP guideline on the achievement ratio of CAP therapy was explored.Results(1)The percentage of patients choosing the first dose antibiotic and following Chinese CAP guideline was 68.8%.There was no significant difference in the percentage in five ...
2011, 32(09)
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Objective To provide effective laboratorial proof and control hospital infection by investigating the distribution and drug resistance of Acinetobacter baumannii and analyzing resistance profiles.Method The distribution,drug resistance and resistance profiles including Aztreonam(A),Ampicillin(P),Ciprofloxacin(C),Meropenem(M),Ceftazidime(F)and Imipenem(I)of Acinetobacter baumannii clinical isolates in our hospital from Jan.2008 to Jun.2011 were reviewed and analyzed by WHONET 5.5 software.Results 878 Acineto...
Objective To provide effective laboratorial proof and control hospital infection by investigating the distribution and drug resistance of Acinetobacter baumannii and analyzing resistance profiles.Method The distribution,drug resistance and resistance profiles including Aztreonam(A),Ampicillin(P),Ciprofloxacin(C),Meropenem(M),Ceftazidime(F)and Imipenem(I)of Acinetobacter baumannii clinical isolates in our hospital from Jan.2008 to Jun.2011 were reviewed and analyzed by WHONET 5.5 software.Results 878 Acineto...
2011, 32(09)
Abstract:
Objective To explore the influence of hyponatremia on older chronic heart failure and effective prevention and control measures.Methods We retrospectively analysed 126 old congestive heart failure patients over 60 in our hospital from January 2006 to June 2011,including 59 patients with hyponatremia(hyponatremia group)and 67 patients with normal blood sodium(control group).We compared the mortality,heart failure grade and the concentration of sodium blood between the two groups,and applied high permeability...
Objective To explore the influence of hyponatremia on older chronic heart failure and effective prevention and control measures.Methods We retrospectively analysed 126 old congestive heart failure patients over 60 in our hospital from January 2006 to June 2011,including 59 patients with hyponatremia(hyponatremia group)and 67 patients with normal blood sodium(control group).We compared the mortality,heart failure grade and the concentration of sodium blood between the two groups,and applied high permeability...
2011, 32(09)
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Objective To observe the clinical effect of combination of isosorbide mononitrate and levamlodipine on hypertension accompanied by coronary heart disease.Methods 30 patients with hypertension accompanied by coronary heart disease were randomly divided into 2 groups:control group and therapy group,15 patients in each group.Patients in both groups received 2.5 mg/d of levamlodipine,and patients in therapy group were also given 60 mg/d of isosorbide mononitrate,the treatment for both groups lasted for 24 weeks...
Objective To observe the clinical effect of combination of isosorbide mononitrate and levamlodipine on hypertension accompanied by coronary heart disease.Methods 30 patients with hypertension accompanied by coronary heart disease were randomly divided into 2 groups:control group and therapy group,15 patients in each group.Patients in both groups received 2.5 mg/d of levamlodipine,and patients in therapy group were also given 60 mg/d of isosorbide mononitrate,the treatment for both groups lasted for 24 weeks...
2011, 32(09)
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Objective To evaluate the value of electronic bronchoscope in diagnosis of lung cancer.Methods We selected and statistically analyzed the data of 302 cases of lung cancer checked by electronic bronchoscope and diagnosed with pathology in the First Affiliated Hospital of Kunming Medical College during 2008 to 2010.According to the gross appearance and microscopic lesions,302 cases were divided into 5 groups: A-neoplasm group;B-invasive group;C-external pressure group;D-inflammation group;E-no abnormal group....
Objective To evaluate the value of electronic bronchoscope in diagnosis of lung cancer.Methods We selected and statistically analyzed the data of 302 cases of lung cancer checked by electronic bronchoscope and diagnosed with pathology in the First Affiliated Hospital of Kunming Medical College during 2008 to 2010.According to the gross appearance and microscopic lesions,302 cases were divided into 5 groups: A-neoplasm group;B-invasive group;C-external pressure group;D-inflammation group;E-no abnormal group....
2011, 32(09)
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Objective To investigate the feasibility and safety of laparoscopic pancreatoduodenectomy.Method The data of one patient undergoing laparoscopic pancreatoduodenectomy in our hospital in June 2011 were studied.Results The operation was successful.No severe intraoperative and postoperative complications were observed.The operation time was 905 minutes and the blood loss was about 600 mL.The time of enteral nutrition by jejunal fistula,recovery of intestinal function,eating by mouth,normal diet by mouth and ho...
Objective To investigate the feasibility and safety of laparoscopic pancreatoduodenectomy.Method The data of one patient undergoing laparoscopic pancreatoduodenectomy in our hospital in June 2011 were studied.Results The operation was successful.No severe intraoperative and postoperative complications were observed.The operation time was 905 minutes and the blood loss was about 600 mL.The time of enteral nutrition by jejunal fistula,recovery of intestinal function,eating by mouth,normal diet by mouth and ho...
2011, 32(09)
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Objective To discuss the prevention and countermeasures of occupational bloodborne exposure risks in HIV health care workers.Methods HIV occupational bloodborne exposure cases in hospital were collected and corresponding management practices and methods were suggested based on the hospital's situation.Results Totally 39 people were exposed to HIV occupational bloodborne exposure injury,of which 21 nurses,8 doctors,6 inspectors and 4 employees;7 primary exposure,13 secondary exposure and 19 tertiary exposure...
Objective To discuss the prevention and countermeasures of occupational bloodborne exposure risks in HIV health care workers.Methods HIV occupational bloodborne exposure cases in hospital were collected and corresponding management practices and methods were suggested based on the hospital's situation.Results Totally 39 people were exposed to HIV occupational bloodborne exposure injury,of which 21 nurses,8 doctors,6 inspectors and 4 employees;7 primary exposure,13 secondary exposure and 19 tertiary exposure...
2011, 32(09)
Abstract:
Objective To provide basis for taking effective measures and methods of improving teaching quality by investigating the status of teaching quality control.Method Randomly sampled teachers were surveyed by self-designed questionnaire.Results The teachers understanding the connotation of total quality management(TQM)accounted for only 3.3%,teaching quality control strength was not enough.Conclusion Understanding of total quality management connotation and teaching quality control efforts should be strengthene...
Objective To provide basis for taking effective measures and methods of improving teaching quality by investigating the status of teaching quality control.Method Randomly sampled teachers were surveyed by self-designed questionnaire.Results The teachers understanding the connotation of total quality management(TQM)accounted for only 3.3%,teaching quality control strength was not enough.Conclusion Understanding of total quality management connotation and teaching quality control efforts should be strengthene...
