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RSS2011 Vol. 32, No. 10
2011, 32(10)
Abstract:
Objective To establish effective method for large-scale purification of islet cells from pig pancreas.Methods Pig pancreas tissue was digested with collagenase P through continuous infusion.Pig islets was purified by Ficoll discontinuous density gradient centrifugation medium.Dithizone(DTZ)staining was used to observe the morphology of islet cells clusters(ICCS),to count islet equivalent IEQ,and to detect the purity of the islet.AO-PI staining was used to detect islet viability and the islet function assess...
Objective To establish effective method for large-scale purification of islet cells from pig pancreas.Methods Pig pancreas tissue was digested with collagenase P through continuous infusion.Pig islets was purified by Ficoll discontinuous density gradient centrifugation medium.Dithizone(DTZ)staining was used to observe the morphology of islet cells clusters(ICCS),to count islet equivalent IEQ,and to detect the purity of the islet.AO-PI staining was used to detect islet viability and the islet function assess...
2011, 32(10)
Abstract:
Objective To explore the antioxidant mechanism of Shuanghuanglian against liver ischemia/reperfusion injury in rats.Methods 50 healthy male SD rats were randomly divided into five groups:(1)normal control group(n=10);(2)I/R group(n=10),the hepatic ischemia-reperfusion model(30 min ischemia,and then 6 h reperfusion);(3)Shuanghuanglian groups(n=30),rats were injected by Shuanghuanglian(1 mL/kg,3 mL/kg and 10 mL/kg,respectively)before made the hepatic I/R model.Rats of all groups were sacrificed after 6 hours....
Objective To explore the antioxidant mechanism of Shuanghuanglian against liver ischemia/reperfusion injury in rats.Methods 50 healthy male SD rats were randomly divided into five groups:(1)normal control group(n=10);(2)I/R group(n=10),the hepatic ischemia-reperfusion model(30 min ischemia,and then 6 h reperfusion);(3)Shuanghuanglian groups(n=30),rats were injected by Shuanghuanglian(1 mL/kg,3 mL/kg and 10 mL/kg,respectively)before made the hepatic I/R model.Rats of all groups were sacrificed after 6 hours....
2011, 32(10)
Abstract:
Objective To observe the effect of compound Guangming capsule in treating retinal light damage.Methods We used the light source of surgical microscope to continuously irradiate the macular of SD rats,to form the model of retinal light damage.Then,all rats were divided into high,medium and low-dose group,control group and model control group.After modeling,all rats accepted OCT examination.Then,rats in high,medium and low-dose group were all fed with different doses of compound Guangming capsule.Fourteen day...
Objective To observe the effect of compound Guangming capsule in treating retinal light damage.Methods We used the light source of surgical microscope to continuously irradiate the macular of SD rats,to form the model of retinal light damage.Then,all rats were divided into high,medium and low-dose group,control group and model control group.After modeling,all rats accepted OCT examination.Then,rats in high,medium and low-dose group were all fed with different doses of compound Guangming capsule.Fourteen day...
2011, 32(10)
Abstract:
Objective To analyze the expression of Survivin in differentiated thyroid carcinomas and discuss the correlation between its expression degree and oncopathological factors.Method The expression of Survivin were detected by immuno-histochemical staining and reverse transcription polymerase chain reaction in differentiated thyroid carcinomas and normal thyroid tissues.Reslults The expression of Survivin mRNA and protein in differentiated thyroid carcinomas was 72.9% and 75%,respectively.No expression of Survi...
Objective To analyze the expression of Survivin in differentiated thyroid carcinomas and discuss the correlation between its expression degree and oncopathological factors.Method The expression of Survivin were detected by immuno-histochemical staining and reverse transcription polymerase chain reaction in differentiated thyroid carcinomas and normal thyroid tissues.Reslults The expression of Survivin mRNA and protein in differentiated thyroid carcinomas was 72.9% and 75%,respectively.No expression of Survi...
2011, 32(10)
Abstract:
Objective To study the expression of GPC3 and AFP in high dysplastic nodules of cirrhosis and hepatocellular carcinoma,and discuss its significance in the hepatocellular carcinoma and high dysplastic nodules of cirrhosis.Method By using immunohistochemical method,we examined the expression of CPC3 and AFP in high dysplastic nodules of cirrhosis,hepatocellular carcinoma and normal liver tissues.Results The positive expression rate of GPC3 was 86.36%(38/44)in advanced HCC;68.18%(15/22)in well-differentiated H...
Objective To study the expression of GPC3 and AFP in high dysplastic nodules of cirrhosis and hepatocellular carcinoma,and discuss its significance in the hepatocellular carcinoma and high dysplastic nodules of cirrhosis.Method By using immunohistochemical method,we examined the expression of CPC3 and AFP in high dysplastic nodules of cirrhosis,hepatocellular carcinoma and normal liver tissues.Results The positive expression rate of GPC3 was 86.36%(38/44)in advanced HCC;68.18%(15/22)in well-differentiated H...
2011, 32(10)
Abstract:
Objective To investigate the protective effect and mechanism of electrical stimulating cerebellar fastigial nucleus on retina against ischemia/reperfusion injury in rats.Methods Rats were divided randomly into three groups:group1(ischemia-reperfusion),group2(electrical-stimulating-treated),and group3(sham-operated).Apoptosis of retinal ganglion cells was detected by TUNEL method,and expression of Bcl-2 and Bax were measured by immunohistochemical staining methods.Results Ischemia/reperfusion injury induced ...
Objective To investigate the protective effect and mechanism of electrical stimulating cerebellar fastigial nucleus on retina against ischemia/reperfusion injury in rats.Methods Rats were divided randomly into three groups:group1(ischemia-reperfusion),group2(electrical-stimulating-treated),and group3(sham-operated).Apoptosis of retinal ganglion cells was detected by TUNEL method,and expression of Bcl-2 and Bax were measured by immunohistochemical staining methods.Results Ischemia/reperfusion injury induced ...
2011, 32(10)
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Objective To discuss the material basis for the pharmacological effects changes of Polygonum multiflorum after microbial fermentation,and study the chemical compositions of Polygonum multiflorum after microbial fermentation.Method After a microbial fermentation,Polygonum multiflorum was extracted and separated by ethyl acetate and n-butyl alcohol,and six chemical constituents was isolated.Result By comparison with the standard preparation analysis and pope analysis,the six chemical constituents were indenti...
Objective To discuss the material basis for the pharmacological effects changes of Polygonum multiflorum after microbial fermentation,and study the chemical compositions of Polygonum multiflorum after microbial fermentation.Method After a microbial fermentation,Polygonum multiflorum was extracted and separated by ethyl acetate and n-butyl alcohol,and six chemical constituents was isolated.Result By comparison with the standard preparation analysis and pope analysis,the six chemical constituents were indenti...
2011, 32(10)
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Objective To observe the clinical therapeutic effect of Modified Chinese Angelica Cold Limbs Decoction on hand-foot syndrome caused by capecitabine.Methods 60 patients treated by capecitabine were divided into two groups randomly and averagely:control group and treatment group.Patients in the control group received chemotherapy treatment routinely,and patients in the treatment group took the Modified Chinese Angelica Cold Limbs Decoction besides chemotherapy treatment.Result 13.3% of patients suffered from ...
Objective To observe the clinical therapeutic effect of Modified Chinese Angelica Cold Limbs Decoction on hand-foot syndrome caused by capecitabine.Methods 60 patients treated by capecitabine were divided into two groups randomly and averagely:control group and treatment group.Patients in the control group received chemotherapy treatment routinely,and patients in the treatment group took the Modified Chinese Angelica Cold Limbs Decoction besides chemotherapy treatment.Result 13.3% of patients suffered from ...
2011, 32(10)
Abstract:
Objective To compare the clinical results of anterior cruciate ligament(ACL) reconstruction with anterior tibial muscle tendons allograft and hamstring tendons autograft.Methods 46 patients who received ACL reconstruction at the Department of Orthopaedics,The First Affiliated Hospital of Kunming Medical College from December of 2008 to August of 2010,including 27 males and 19 females,were divided into 2 groups:group A(25 patients,used Hamstring Tendon Autograft)and group B(21 patients,used Anterior Tibial M...
Objective To compare the clinical results of anterior cruciate ligament(ACL) reconstruction with anterior tibial muscle tendons allograft and hamstring tendons autograft.Methods 46 patients who received ACL reconstruction at the Department of Orthopaedics,The First Affiliated Hospital of Kunming Medical College from December of 2008 to August of 2010,including 27 males and 19 females,were divided into 2 groups:group A(25 patients,used Hamstring Tendon Autograft)and group B(21 patients,used Anterior Tibial M...
2011, 32(10)
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Objective To observe the clinical effect of Xuebijing Injection on pro/anti-inflammatory balance in sepsis patients.Methods According to recruitment criterions,21 patients with severe sepsis and multiple organ dysfunction syndrome from October,2009 to October,2010 in emergency intensive care unit were divided randomly into treatment group and control group.Patients in the treatment group were combined with Xuebijing injection(100 mL Q12 h),and patients in the control group were combined with Sodium Chloride...
Objective To observe the clinical effect of Xuebijing Injection on pro/anti-inflammatory balance in sepsis patients.Methods According to recruitment criterions,21 patients with severe sepsis and multiple organ dysfunction syndrome from October,2009 to October,2010 in emergency intensive care unit were divided randomly into treatment group and control group.Patients in the treatment group were combined with Xuebijing injection(100 mL Q12 h),and patients in the control group were combined with Sodium Chloride...
2011, 32(10)
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Objective To evaluate the therapeutic effect of Hong Jin Xiao Jie capsule combined with physical therapy on mammary hyperplasia.Methods 197 cases of mammary hyperplasia were divided into three groups.The group A with 98 cases was treated by Hong Jin Xiao Jie capsule and physical therapy.The group B with 51 cases was treated by Hong Jin Xiao Jie capsule.The group C with 48 cases was treated by physical therapy.Results In the group B and C,the total effective rate was 84.3% and 83.3%.There was no significant ...
Objective To evaluate the therapeutic effect of Hong Jin Xiao Jie capsule combined with physical therapy on mammary hyperplasia.Methods 197 cases of mammary hyperplasia were divided into three groups.The group A with 98 cases was treated by Hong Jin Xiao Jie capsule and physical therapy.The group B with 51 cases was treated by Hong Jin Xiao Jie capsule.The group C with 48 cases was treated by physical therapy.Results In the group B and C,the total effective rate was 84.3% and 83.3%.There was no significant ...
2011, 32(10)
Abstract:
Objective To analyze the influence of left subclavian artery(LSA)coverage on the blood-supply of brain and upper extremity during the endovascular repair(EVR)of Stanford type B aortic dissection(AD).Method We retrospectively analyzed these clinical and follow-up data of 126 patients with type B dissection who were treated by EVR.Results Among 126 patients with Stanford type B AD who received the endovascular repair(EVR),86 patients' LSA were covered fully or partly.35 patients,LSA were complete covered and ...
Objective To analyze the influence of left subclavian artery(LSA)coverage on the blood-supply of brain and upper extremity during the endovascular repair(EVR)of Stanford type B aortic dissection(AD).Method We retrospectively analyzed these clinical and follow-up data of 126 patients with type B dissection who were treated by EVR.Results Among 126 patients with Stanford type B AD who received the endovascular repair(EVR),86 patients' LSA were covered fully or partly.35 patients,LSA were complete covered and ...
2011, 32(10)
Abstract:
Objective To explore the association between 420 C/G polymorphism of resistin and hypertension in T2DM patients.Methods 72 healthy subjects,40 T2DM patients and 40 patients with T2DM and hypertension were selected in normal control group,T2DM group and T2DM and hypertension group,respectively.The single nucleotide polymorphism(SNP)of resistin in three groups was demonstrated by PCR and restriction endonuclease digestion.The genotype frequency and allele frequency were compared among three groups.Results(1)T...
Objective To explore the association between 420 C/G polymorphism of resistin and hypertension in T2DM patients.Methods 72 healthy subjects,40 T2DM patients and 40 patients with T2DM and hypertension were selected in normal control group,T2DM group and T2DM and hypertension group,respectively.The single nucleotide polymorphism(SNP)of resistin in three groups was demonstrated by PCR and restriction endonuclease digestion.The genotype frequency and allele frequency were compared among three groups.Results(1)T...
2011, 32(10)
Abstract:
Objective To investigate the plasma concentration of visfatin in obesity patients and non-obesity controls and its correlation with the metabolic characteristics of obesity.Methods The height,body weight,waistline,and blood pressure of 171 healthy subjects were measured,and the BMI and WHR were calculated.The serum levels of FPG,Fins,TC,TG,LDL-c and HDL-c were also measured.The serum level of Visfatin was measured with ELISA assay,and its correlation with BMI,WHR and metabolic indexes was analyzed.Results(1...
Objective To investigate the plasma concentration of visfatin in obesity patients and non-obesity controls and its correlation with the metabolic characteristics of obesity.Methods The height,body weight,waistline,and blood pressure of 171 healthy subjects were measured,and the BMI and WHR were calculated.The serum levels of FPG,Fins,TC,TG,LDL-c and HDL-c were also measured.The serum level of Visfatin was measured with ELISA assay,and its correlation with BMI,WHR and metabolic indexes was analyzed.Results(1...
2011, 32(10)
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Objective To study the changes of triglyceride(TG),total cholesterol(TC),high density lipoprotein cholesterol(H-DLC)and low density lipoprotein cholesterol(L-DLC)in patients with acute myocardial infarction(AMI)and the influencing factors.Methods By refering to medical records,this study researched the blood lipids changes in AMI patients(as the studying group)and patients with coronary heart disease(CHD)(as the control group).By questionnaires,this study researched some information about treatment and the ...
Objective To study the changes of triglyceride(TG),total cholesterol(TC),high density lipoprotein cholesterol(H-DLC)and low density lipoprotein cholesterol(L-DLC)in patients with acute myocardial infarction(AMI)and the influencing factors.Methods By refering to medical records,this study researched the blood lipids changes in AMI patients(as the studying group)and patients with coronary heart disease(CHD)(as the control group).By questionnaires,this study researched some information about treatment and the ...
2011, 32(10)
Abstract:
Objective To investigate reproductive outcome after different management strategies for tubal pregnancy.Method A retrospective study was carried out to analyse the reproductive outcome of 176 cases after different management strategies for tubal pregnancy.Results The intrauterine pregnancy rate was 59.6% in surgery group and 52.8% in medical treatment group.The recurrent ectopic pregnancy rate was 14.4% in surgery group and 13.9% in medical treatment group.The infertility rate was 26.0% in surgery group and...
Objective To investigate reproductive outcome after different management strategies for tubal pregnancy.Method A retrospective study was carried out to analyse the reproductive outcome of 176 cases after different management strategies for tubal pregnancy.Results The intrauterine pregnancy rate was 59.6% in surgery group and 52.8% in medical treatment group.The recurrent ectopic pregnancy rate was 14.4% in surgery group and 13.9% in medical treatment group.The infertility rate was 26.0% in surgery group and...
2011, 32(10)
Abstract:
Objective To explore the relationship between histological type and gender,age,as well as tobacco smoking of patients with lung cancer diagnosed by bronchoscope.To lay the foundation for expanding the use of bronchoscope in diagnosing all kinds of malignant tumor of respiratory system.Method We selected the data of 225 cases checked by bronchoscope and diagnosed with pathology of lung cancer in the First Affiliated Hospital of Kunming Medical College during 2008 to 2010 and statistically analyzed.Results In...
Objective To explore the relationship between histological type and gender,age,as well as tobacco smoking of patients with lung cancer diagnosed by bronchoscope.To lay the foundation for expanding the use of bronchoscope in diagnosing all kinds of malignant tumor of respiratory system.Method We selected the data of 225 cases checked by bronchoscope and diagnosed with pathology of lung cancer in the First Affiliated Hospital of Kunming Medical College during 2008 to 2010 and statistically analyzed.Results In...
2011, 32(10)
Abstract:
Objective To investigate the clinical effect of pancreatoduodenectomy.Methods The clinical data of 48 patients who were performed pancreatoduodenectomy in our hospital from October 2002 to October 2010 were analysed retrospectively.Results There was no operative mortality in all cases.16 patients were performed preoperative biliary drainage,and 18 patients were performed jejunostomy.Postoperative pathological examination:carcinoma of head of pancreas in 8 patients,distal common bile duct carcinoma in 7 pati...
Objective To investigate the clinical effect of pancreatoduodenectomy.Methods The clinical data of 48 patients who were performed pancreatoduodenectomy in our hospital from October 2002 to October 2010 were analysed retrospectively.Results There was no operative mortality in all cases.16 patients were performed preoperative biliary drainage,and 18 patients were performed jejunostomy.Postoperative pathological examination:carcinoma of head of pancreas in 8 patients,distal common bile duct carcinoma in 7 pati...
2011, 32(10)
Abstract:
Objective To evaluate the clinical effects of Jinhoujian spry mixing calcium hydroxide paste on chronic periapical periodontitis.Methods 196 teeth with chronic periapical periodontitis were selected,and randomly divided into two groups: experimental group and control group.After root canel preparing,the root canels of teeth in the experimental group were enveloped with Jinhoujian spry mixing calcium hydroxide paste and the control group were enveloped with calcium hydroxide paste.The clinical effects were e...
Objective To evaluate the clinical effects of Jinhoujian spry mixing calcium hydroxide paste on chronic periapical periodontitis.Methods 196 teeth with chronic periapical periodontitis were selected,and randomly divided into two groups: experimental group and control group.After root canel preparing,the root canels of teeth in the experimental group were enveloped with Jinhoujian spry mixing calcium hydroxide paste and the control group were enveloped with calcium hydroxide paste.The clinical effects were e...
2011, 32(10)
Abstract:
Objective To investigate the clinical curative effect of Urinary Kallidinogenase on the changes of serum C-reactive protein(CRP)and IL-8 in patients with acute cerebral infarction(ACI).Methods 66 acute cerebral infarction patients were randomly divided into two groups:treatment group and control group.Patients in both groups were given cerebral nervenutriceutical and antiplatelet routine treatment.Urinary Kallidinogenase was additionally used in the treatment group.The neurologic impairment scores and serum...
Objective To investigate the clinical curative effect of Urinary Kallidinogenase on the changes of serum C-reactive protein(CRP)and IL-8 in patients with acute cerebral infarction(ACI).Methods 66 acute cerebral infarction patients were randomly divided into two groups:treatment group and control group.Patients in both groups were given cerebral nervenutriceutical and antiplatelet routine treatment.Urinary Kallidinogenase was additionally used in the treatment group.The neurologic impairment scores and serum...
2011, 32(10)
Abstract:
Objective To study the difference of microscopic hematuria before and after kidney biopsy and the influence on the therapy.Method We evaluated 282 patients' microscopic hematuria before and after kidney biopsy in our department from January 2008 to July 2011 and discussed the difference.Results 282 cases were chronic glomerulonephritis(including asymptomatic hematuria and proteinuria syndrome) and essential nephritic syndrome,secondary kidney disease was lupus nephritis,mostly.Clinical manifestations were h...
Objective To study the difference of microscopic hematuria before and after kidney biopsy and the influence on the therapy.Method We evaluated 282 patients' microscopic hematuria before and after kidney biopsy in our department from January 2008 to July 2011 and discussed the difference.Results 282 cases were chronic glomerulonephritis(including asymptomatic hematuria and proteinuria syndrome) and essential nephritic syndrome,secondary kidney disease was lupus nephritis,mostly.Clinical manifestations were h...
2011, 32(10)
Abstract:
Objective To study the relationship between the expression of KI-67and Survivin in cervical carcinoma and the clinical feature of cervical carcinoma and to observe the correlation between KI-67 and Survivin in cervical carcinoma.Methods Using streptavidin-biotin peroxidase(SP)method,we examined the expression of KI-67and Survivin in 73 cases of cervical carcinoma,25 cases of cervical intraepithelial neoplasia(CIN)and 20 cases of chronic cervicitis.Results The expression of KI-67 and Survivin presented a gro...
Objective To study the relationship between the expression of KI-67and Survivin in cervical carcinoma and the clinical feature of cervical carcinoma and to observe the correlation between KI-67 and Survivin in cervical carcinoma.Methods Using streptavidin-biotin peroxidase(SP)method,we examined the expression of KI-67and Survivin in 73 cases of cervical carcinoma,25 cases of cervical intraepithelial neoplasia(CIN)and 20 cases of chronic cervicitis.Results The expression of KI-67 and Survivin presented a gro...
2011, 32(10)
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Objective To investigate platelet mobilizing function,blood clotting function and platelet correlated parametric detection index of rectal cancer patients and healthy volunteers,to dentify correlation of rectal carcinoma and blood hypercoagulable state.Methods We selected 89 rectal cancer patients confirmed by pathology as observation group and 40 healthy volunteers as the control.The expression of CD62P and CD63 in activated platelets,blood clotting function(TT,PT,APTT and FIB)and platelet correlated param...
Objective To investigate platelet mobilizing function,blood clotting function and platelet correlated parametric detection index of rectal cancer patients and healthy volunteers,to dentify correlation of rectal carcinoma and blood hypercoagulable state.Methods We selected 89 rectal cancer patients confirmed by pathology as observation group and 40 healthy volunteers as the control.The expression of CD62P and CD63 in activated platelets,blood clotting function(TT,PT,APTT and FIB)and platelet correlated param...
2011, 32(10)
Abstract:
Objective To explore the feasibility of emergency laparoscopic cholecystectomy and to summarize the operative technique.Method The data of 67 patients with acute cholecystitis received emergency laparoscopic cholecystectomy were analyzed retrospectively.Results Among the 67 cases,64 patients were treated by LC,3 patients were converted to open surgery.The mean operative time of LC was 56 min,mean operative blood loss was 80 mL,16 patients were treated by absorbable hemostatic gauze because of bleeding,18 pa...
Objective To explore the feasibility of emergency laparoscopic cholecystectomy and to summarize the operative technique.Method The data of 67 patients with acute cholecystitis received emergency laparoscopic cholecystectomy were analyzed retrospectively.Results Among the 67 cases,64 patients were treated by LC,3 patients were converted to open surgery.The mean operative time of LC was 56 min,mean operative blood loss was 80 mL,16 patients were treated by absorbable hemostatic gauze because of bleeding,18 pa...
2011, 32(10)
Abstract:
Objective To explore the clinical value of plasma BNP in the diagnosis of heart failure after acute myocardial infarction.Methods We retrospectively analyzed the data of 75 patients with acute myocardial infarction,compared BNP value before and after treatment among different cardiac function groups,and between low blood pressure and heart failure group and low blood pressure and insufficient blood capacity group.Results Patients in different cardiac function group have different BNP values,the worse the he...
Objective To explore the clinical value of plasma BNP in the diagnosis of heart failure after acute myocardial infarction.Methods We retrospectively analyzed the data of 75 patients with acute myocardial infarction,compared BNP value before and after treatment among different cardiac function groups,and between low blood pressure and heart failure group and low blood pressure and insufficient blood capacity group.Results Patients in different cardiac function group have different BNP values,the worse the he...
