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RSS2012 Vol. 33, No. 02
2012, 33(02)
Abstract:
［Abstract］Objective To investigate the inhibitory effects of flavonoid isolated from the leaves of Acer truncatum Bunge on the expression of pro-inflammatory cytokines in Raw264.7 cells stimulated with lipopolysaccharide （LPS）．Methods Inflammatory cell model was structured by LPS-stimulated Raw264.7 cells，the cells were treated with flavonoid（10 μM， 30 μM and 60 μM） prior to LPS（5 mg/mL）exposure and the effects of flavonoid on viability of Raw264.7 cells were measured by MTT assay，Nitric oxide（NO）and prostaglandin E2 （PGE2）were measured by ELISA，the effects on the protein levels of pro-inflammatory enzymes，cyclooxygenase-2（COX-2）and inducible nitric oxide synthase（iNOS），and pro-inflammatory cytokines，tumor necrosis factor-α（TNF-α），and interleukin-1β（IL-1β），inflammatory signaling proteins nuclear factor-κB （NF-κB）were analysed by immunofl uorescence staining assay．Results LPS-induced NO，PGE2，iNOS， COX-2 and NF-κB protein expression levels were significantly decreased by flavonoid in a concentration-dependent manner and flavonoid also had an effect on the expression of TNF-α，IL-1β through translational inhibition． Conclusion Flavonoid isolated from the leaves of Acer truncatum Bunge can inhibit LPS-induced activation of Raw264.7 cells through inhibiting the release of pro-infl ammatory mediators，then exerts its anti-osteoporosis activities.
［Abstract］Objective To investigate the inhibitory effects of flavonoid isolated from the leaves of Acer truncatum Bunge on the expression of pro-inflammatory cytokines in Raw264.7 cells stimulated with lipopolysaccharide （LPS）．Methods Inflammatory cell model was structured by LPS-stimulated Raw264.7 cells，the cells were treated with flavonoid（10 μM， 30 μM and 60 μM） prior to LPS（5 mg/mL）exposure and the effects of flavonoid on viability of Raw264.7 cells were measured by MTT assay，Nitric oxide（NO）and prostaglandin E2 （PGE2）were measured by ELISA，the effects on the protein levels of pro-inflammatory enzymes，cyclooxygenase-2（COX-2）and inducible nitric oxide synthase（iNOS），and pro-inflammatory cytokines，tumor necrosis factor-α（TNF-α），and interleukin-1β（IL-1β），inflammatory signaling proteins nuclear factor-κB （NF-κB）were analysed by immunofl uorescence staining assay．Results LPS-induced NO，PGE2，iNOS， COX-2 and NF-κB protein expression levels were significantly decreased by flavonoid in a concentration-dependent manner and flavonoid also had an effect on the expression of TNF-α，IL-1β through translational inhibition． Conclusion Flavonoid isolated from the leaves of Acer truncatum Bunge can inhibit LPS-induced activation of Raw264.7 cells through inhibiting the release of pro-infl ammatory mediators，then exerts its anti-osteoporosis activities.
2012, 33(02)
Abstract:
［Abstract］Objective To investigate the effective agents in the Valeriana Jatamansii Jones and 5 drugs for treatment of viral enteritis．Methods The content of 10 oligosaccharides in 6 drugs including Valeriana Jatamansii Jones，Dictahedral Smectite Powder，Berberine Hydrochloride Tablets，Compound Glutamin Entersoluble capsule，Shenling Jianpiwei Keli，L-Glutamine and Sodium Gualenate Granules for gastroenteral diseases was determined by DIONEX CS-3000 high performance liquid chromatography-iron analyzer in the study．Results The results showed 10 oligosaccharides were rich in the Compound Glutamin Entersoluble capsule，Valeriana Jatamansii Jones and Shenling Jianpiwei Keli．There was no oligosaccharides in the L-Glutamine and Sodium Gualenate Granules．There was higher content of the galactose in the Shenling Jianpiwei Keli，the Montmorillonite powder and Valeriana Jatamansii Jones，especially the galactose was rich in the Shenling Jianpiwei Keli．The verbascoses were rich in the Valeriana Jatamansii Jones．There were higher content of maltotetraose Dp4 and 7 in the Compound Glutamin Entersoluble capsule．Conclusions The oligosaccharides are rich in the Valeriana Jatamansii Jones，Compound Glutamin Entersoluble capsule，Shenling Jianpiwei Keli and montmorillonite powder．The verbascoses especially are rich in the Valeriana Jatamansii Jones．The higher content of the oligosaccharides in these drugs may be associated with improving intestinal micrflora and functions.
［Abstract］Objective To investigate the effective agents in the Valeriana Jatamansii Jones and 5 drugs for treatment of viral enteritis．Methods The content of 10 oligosaccharides in 6 drugs including Valeriana Jatamansii Jones，Dictahedral Smectite Powder，Berberine Hydrochloride Tablets，Compound Glutamin Entersoluble capsule，Shenling Jianpiwei Keli，L-Glutamine and Sodium Gualenate Granules for gastroenteral diseases was determined by DIONEX CS-3000 high performance liquid chromatography-iron analyzer in the study．Results The results showed 10 oligosaccharides were rich in the Compound Glutamin Entersoluble capsule，Valeriana Jatamansii Jones and Shenling Jianpiwei Keli．There was no oligosaccharides in the L-Glutamine and Sodium Gualenate Granules．There was higher content of the galactose in the Shenling Jianpiwei Keli，the Montmorillonite powder and Valeriana Jatamansii Jones，especially the galactose was rich in the Shenling Jianpiwei Keli．The verbascoses were rich in the Valeriana Jatamansii Jones．There were higher content of maltotetraose Dp4 and 7 in the Compound Glutamin Entersoluble capsule．Conclusions The oligosaccharides are rich in the Valeriana Jatamansii Jones，Compound Glutamin Entersoluble capsule，Shenling Jianpiwei Keli and montmorillonite powder．The verbascoses especially are rich in the Valeriana Jatamansii Jones．The higher content of the oligosaccharides in these drugs may be associated with improving intestinal micrflora and functions.
2012, 33(02)
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［Abstract］Objective To establish a rat model of aldosterone-producing adenoma（APA）. Methods 16 Sprague-Dawley rats subcutaneously implanted with an osmotic mini-pump，were randomly divided into 2 groups： control group（vehicle）and APA（1 μg/h）group．Because the mini-pump（ALZET 2004）could continuously infuse test substance for 4 weeks，another minipump was replaced after the fourth week to make the duration of aldosterone infusion for 8 weeks．Systolic blood pressure（SBP）was measured weekly by the tail-cuff method．At the termination of the study，blood was collected for measurements of plasma renin activity（PRA）and aldosterone concontration（PAC）with radioimmunoassay kits．Results After 3 weeks，APA rats showed significantly and progressively increased SBP compared with controls（P＜0.05），and the SBP level reached peak on 7th week and remained high levels．PRA was substantially depressed and PAC was significantly raised in APA rats（P＜0.01）． Conclusion By implanting osmotic minipumps subcutaneously， the rat models of APA can be successfully established， which provide a good platform for investigating the pathogenesis of APA.
［Abstract］Objective To establish a rat model of aldosterone-producing adenoma（APA）. Methods 16 Sprague-Dawley rats subcutaneously implanted with an osmotic mini-pump，were randomly divided into 2 groups： control group（vehicle）and APA（1 μg/h）group．Because the mini-pump（ALZET 2004）could continuously infuse test substance for 4 weeks，another minipump was replaced after the fourth week to make the duration of aldosterone infusion for 8 weeks．Systolic blood pressure（SBP）was measured weekly by the tail-cuff method．At the termination of the study，blood was collected for measurements of plasma renin activity（PRA）and aldosterone concontration（PAC）with radioimmunoassay kits．Results After 3 weeks，APA rats showed significantly and progressively increased SBP compared with controls（P＜0.05），and the SBP level reached peak on 7th week and remained high levels．PRA was substantially depressed and PAC was significantly raised in APA rats（P＜0.01）． Conclusion By implanting osmotic minipumps subcutaneously， the rat models of APA can be successfully established， which provide a good platform for investigating the pathogenesis of APA.
2012, 33(02)
Abstract:
［Abstract］Objective To prove the inhibition effects of combination of indomethacin and tea polyphenols on growth of Lewis pulmonary tumor（LLC）in mice．Methods LLC transplant model was built in mice．The model mice were divided randomly into 4 groups：control group，indomethacin group，tea polyphenols group and combination administration group．The tumor volume change and tumor suppressor rate of LLC in mice in four groups were observed．Results The indomethacin and tea polyphenols group had obvious inhibiting effect on the transplanted Lewis pulmonary tumor in mice，compared with control group（P＜0.05）．The inhibiting effect was enormously enhanced by drug combination of indomethacin and tea polyphenols．Conclusions Combination of indomethacin and tea polyphenols has obvious inhibition effect on the tumor growth of transplanted Lewis pulmonary tumor in mice，which could significantly enhance the inhibition effect to a certain degree，compared with independent administration.
［Abstract］Objective To prove the inhibition effects of combination of indomethacin and tea polyphenols on growth of Lewis pulmonary tumor（LLC）in mice．Methods LLC transplant model was built in mice．The model mice were divided randomly into 4 groups：control group，indomethacin group，tea polyphenols group and combination administration group．The tumor volume change and tumor suppressor rate of LLC in mice in four groups were observed．Results The indomethacin and tea polyphenols group had obvious inhibiting effect on the transplanted Lewis pulmonary tumor in mice，compared with control group（P＜0.05）．The inhibiting effect was enormously enhanced by drug combination of indomethacin and tea polyphenols．Conclusions Combination of indomethacin and tea polyphenols has obvious inhibition effect on the tumor growth of transplanted Lewis pulmonary tumor in mice，which could significantly enhance the inhibition effect to a certain degree，compared with independent administration.
2012, 33(02)
Abstract:
［Abstract］Objective To study effects of luteolin on isolated heart function and oxidative stress reaction after hypothermic preservation．Methods Forty male SD rats were randomly divided into 2 groups（n＝20 in each group）：control group（heart preservative solution was UW solution） and experiment group （added 30 μmol/L luteolin in UW solution ）．Langendorff model of isolated rat heart was used．The cardiac contractile function and diastolic function were determined 18 hours after hypothermic preservation followed by 60 min reperfusion．The amount of creatine kinase（CK）and lactic dehydrogenase（LDH）leakage in the coronary effluent，the content of Superoxide Dismutase（SOD）and malondialdehyde（MDA）in myocardial tissue were measured．Results Luteolin significantly improved the recovery of cardiac contractile function（LVPSP，+dp/dtmax）and diastolic function（-dp/dtmax）after hypothermic preservation，reduced the leakage of CK and LDH in the coronary effluent，reduced the content of MDA in myocardial tissue and increase the content of SOD in myocardial tissue， compared with control group．Conclusion Luteolin can significantly improve isolated heart function and reduce oxidative stress reaction in myocardial tissue after hypothermic preservation.
［Abstract］Objective To study effects of luteolin on isolated heart function and oxidative stress reaction after hypothermic preservation．Methods Forty male SD rats were randomly divided into 2 groups（n＝20 in each group）：control group（heart preservative solution was UW solution） and experiment group （added 30 μmol/L luteolin in UW solution ）．Langendorff model of isolated rat heart was used．The cardiac contractile function and diastolic function were determined 18 hours after hypothermic preservation followed by 60 min reperfusion．The amount of creatine kinase（CK）and lactic dehydrogenase（LDH）leakage in the coronary effluent，the content of Superoxide Dismutase（SOD）and malondialdehyde（MDA）in myocardial tissue were measured．Results Luteolin significantly improved the recovery of cardiac contractile function（LVPSP，+dp/dtmax）and diastolic function（-dp/dtmax）after hypothermic preservation，reduced the leakage of CK and LDH in the coronary effluent，reduced the content of MDA in myocardial tissue and increase the content of SOD in myocardial tissue， compared with control group．Conclusion Luteolin can significantly improve isolated heart function and reduce oxidative stress reaction in myocardial tissue after hypothermic preservation.
2012, 33(02)
Abstract:
［Abstract］Objective To study the effects of neural stem cells transplantation on spinal nerves electrophysiology in subacute stage．Methods 15 femal adult rats were divided into three groups：sham operation group， merely spinal cord transsection group，and NSC transplantation group．The cortical somatosensory evoked potential（CSEP）and Motion Evoked Potential（MEP）were evaluated and the cortical motor area stimulation by electro-acupuncture was performed in the three groups．Results Latency of CSEP and MEP of rats in NSC transplantation group was shorter than merely spinal cord transsection group．Neural stem cells transplantation led to hind limbs contraction after cortical motor area stimulation by electro-acupuncture．Conclusion Neural stem cells transplantation can partially promotes the recovery of sensory nerve and motor nerve conduction of injured spinal cord.
［Abstract］Objective To study the effects of neural stem cells transplantation on spinal nerves electrophysiology in subacute stage．Methods 15 femal adult rats were divided into three groups：sham operation group， merely spinal cord transsection group，and NSC transplantation group．The cortical somatosensory evoked potential（CSEP）and Motion Evoked Potential（MEP）were evaluated and the cortical motor area stimulation by electro-acupuncture was performed in the three groups．Results Latency of CSEP and MEP of rats in NSC transplantation group was shorter than merely spinal cord transsection group．Neural stem cells transplantation led to hind limbs contraction after cortical motor area stimulation by electro-acupuncture．Conclusion Neural stem cells transplantation can partially promotes the recovery of sensory nerve and motor nerve conduction of injured spinal cord.
2012, 33(02)
Abstract:
［Abstract］Objective To investigate the expression of 3-phosphoinositide-dependent protein kinase 1（PDK1） in skeletal muscle tissue of hyherhomocysteinemia animal model， to explore the effect of homocysteine on glucose uptake．Methods Twenty healthy mice，six weeks of age，were randomly divided into control group（n = 10）and high hyperhomocysteinemia group（n =10）．Normal food was fed in control group；Normal food and 1.5 % （mass fraction）methionine were fed in hyperhomocysteinemia group．After the feed in the hyperhomocysteinemia group and the control group were fed for three months，the levels of blood glucose were determined in the feeding． The variations of PDK1，phosphorylation of PDK1 and phosphorylation of Akt were observed with Immunohistochemistry in skeletal muscle cells．The expressions of PDK1，phosphorylation of PDK1，Akt and phosphorylation of Akt were determined with Western Blot in skeletal muscle tissue．Results The feeding plasma glucose in the hyperhomocysteinemia group were higher than that in the control group（P＜0.05）．The gray values of PDK1，p- PDK1（Ser-241）and p-Akt（Thr-308）were increased in the herpyhomocysteinemia group with Immunohistochemistry compared with the control group（P＜0.05）．The levels of PDK1，p- PDK1（Ser-241）and p-Akt（Thr-308）were the lower in the homocysteinemia group with Western Blot（P＜0.05）．However，the expression of Akt protein was not difference between the homocysteinemia group and the control group（P＞0.05）． Conclusion Homocysteine may decrease the expression of PDK1 and phosphorylation of PDK1 in skeletal muscle tissue，thus inhibits glucose uptake.
［Abstract］Objective To investigate the expression of 3-phosphoinositide-dependent protein kinase 1（PDK1） in skeletal muscle tissue of hyherhomocysteinemia animal model， to explore the effect of homocysteine on glucose uptake．Methods Twenty healthy mice，six weeks of age，were randomly divided into control group（n = 10）and high hyperhomocysteinemia group（n =10）．Normal food was fed in control group；Normal food and 1.5 % （mass fraction）methionine were fed in hyperhomocysteinemia group．After the feed in the hyperhomocysteinemia group and the control group were fed for three months，the levels of blood glucose were determined in the feeding． The variations of PDK1，phosphorylation of PDK1 and phosphorylation of Akt were observed with Immunohistochemistry in skeletal muscle cells．The expressions of PDK1，phosphorylation of PDK1，Akt and phosphorylation of Akt were determined with Western Blot in skeletal muscle tissue．Results The feeding plasma glucose in the hyperhomocysteinemia group were higher than that in the control group（P＜0.05）．The gray values of PDK1，p- PDK1（Ser-241）and p-Akt（Thr-308）were increased in the herpyhomocysteinemia group with Immunohistochemistry compared with the control group（P＜0.05）．The levels of PDK1，p- PDK1（Ser-241）and p-Akt（Thr-308）were the lower in the homocysteinemia group with Western Blot（P＜0.05）．However，the expression of Akt protein was not difference between the homocysteinemia group and the control group（P＞0.05）． Conclusion Homocysteine may decrease the expression of PDK1 and phosphorylation of PDK1 in skeletal muscle tissue，thus inhibits glucose uptake.
2012, 33(02)
Abstract:
［Abstract］Objective To investigate the current status of college students smoking behavior and explore the influencing factors for developing targeted intervention methods．Methods Questionnaire interview and focus group discussion were used to investigate 1 064 students who were drawn by multi-stage stratified cluster random sampling in 3 universities in Kunming of Yunnan Province．The data were analyzed using Spss 11.0 package．Results Smoking rates among students in 3 universities was 9.1%．Result showed the relative high levels of smoking rates among males of 16.3% compared to the female of 0.6%．First smoking average age was 15.7．There was no significance difference in smoking rate among three universities．Monthly consume in cigarette，knowledge of lung cancer related smoking， friends smoking， grade in school， father smoking，mother's job and sex were influencing factors．Conclusions Although smoking rate is lower than other research’s report，comprehensive intervention should be considered，such as higher cigarette tax rate，health education，no smoking family，etc. Intervention should pay more attention to medical students especially.
［Abstract］Objective To investigate the current status of college students smoking behavior and explore the influencing factors for developing targeted intervention methods．Methods Questionnaire interview and focus group discussion were used to investigate 1 064 students who were drawn by multi-stage stratified cluster random sampling in 3 universities in Kunming of Yunnan Province．The data were analyzed using Spss 11.0 package．Results Smoking rates among students in 3 universities was 9.1%．Result showed the relative high levels of smoking rates among males of 16.3% compared to the female of 0.6%．First smoking average age was 15.7．There was no significance difference in smoking rate among three universities．Monthly consume in cigarette，knowledge of lung cancer related smoking， friends smoking， grade in school， father smoking，mother's job and sex were influencing factors．Conclusions Although smoking rate is lower than other research’s report，comprehensive intervention should be considered，such as higher cigarette tax rate，health education，no smoking family，etc. Intervention should pay more attention to medical students especially.
2012, 33(02)
Abstract:
［Abstract］Objective To find out medical college students’ health psychological status and provide evidence for their psychological health education and prevention．Method 487 students of Kunming Medical University were investigated by SCL-90 questionare．Results The students’ psychological health status is worse than normal level．The psychological health status of male is similar to female．The psychological health levels of students with different majors are：Clinical Medicine＞Nursing＞Preventive Medicine．Conclusion It is necessary to put more attention on the psychological health instruction to the medical college students. Meanwhile，we should advance students’ constitution and psychological health by means of P.E.
［Abstract］Objective To find out medical college students’ health psychological status and provide evidence for their psychological health education and prevention．Method 487 students of Kunming Medical University were investigated by SCL-90 questionare．Results The students’ psychological health status is worse than normal level．The psychological health status of male is similar to female．The psychological health levels of students with different majors are：Clinical Medicine＞Nursing＞Preventive Medicine．Conclusion It is necessary to put more attention on the psychological health instruction to the medical college students. Meanwhile，we should advance students’ constitution and psychological health by means of P.E.
2012, 33(02)
Abstract:
［Abstract］Objective To explore the current status influencing factors of health behavior among adolescents in Wuhua district of Kunming city and provide policy-making evidence for government．Methods National questionnaire of Chinese adolescent health behavioral was used to investigate students in 6 randomly selected schools．Data were analyzed using SPSS 13.0．Results Risky behavior was popular among adolescents in this district．Smoking rate was 35.7%，drinking alcohol rate was 73.8%. 23% students had gambling behavior ever．Conclusion In order to improve health states of adolescents，comprehensive intervention measures including health education should be taken to enhance awareness and change adolescent risky behavior.
［Abstract］Objective To explore the current status influencing factors of health behavior among adolescents in Wuhua district of Kunming city and provide policy-making evidence for government．Methods National questionnaire of Chinese adolescent health behavioral was used to investigate students in 6 randomly selected schools．Data were analyzed using SPSS 13.0．Results Risky behavior was popular among adolescents in this district．Smoking rate was 35.7%，drinking alcohol rate was 73.8%. 23% students had gambling behavior ever．Conclusion In order to improve health states of adolescents，comprehensive intervention measures including health education should be taken to enhance awareness and change adolescent risky behavior.
2012, 33(02)
Abstract:
［Abstract］Objective To explore the correlation between lower extremity atherosclerosis（LEA）and bone mineral density in type 2 diabetes mellitus（T2-DM）patients．Methods Two hundred and nineteen T2-DM were enrolled in this study．Severity of LEA was examined by Doppler Ultrasound of Aloka -α 10（Japan）. According to severity of LEA score，the subjects were divided into the control（n＝65），mild（n＝62），moderate （n=56）and severe（n=36） groups. The bone mineral density （BMD）in lumbar vertebrate and femoral bone was measured by Dual-energy X-ray absorptiometry（ DEXA）made in America．The clinical factors and biochemical parameters of patients in different groups were observed and the correlation between lower extremity atherosclerosis and bone mineral density．Results（1） With the increase in the severity of LEA，the HBA1c，FBG， 2HPBG， TC， TG and LDL were gradually increased ，but HDL was decreased in the four groups， there was significant differences（P＜0.05）.（2）The severity score of LEA was negative correlated with the BMD in lumbar vertebrat and femoral bone.（3）Multivariate Logistic regression analysis showed the change of BMD，HBA1c and TC were major risk factors of LEA T2-DM .Conclusions With the increase in the severity of LEA in T2-DM，the decrease of BMD is more obvious．The decrease of BMD is a risk factor of LEA production and development．
［Abstract］Objective To explore the correlation between lower extremity atherosclerosis（LEA）and bone mineral density in type 2 diabetes mellitus（T2-DM）patients．Methods Two hundred and nineteen T2-DM were enrolled in this study．Severity of LEA was examined by Doppler Ultrasound of Aloka -α 10（Japan）. According to severity of LEA score，the subjects were divided into the control（n＝65），mild（n＝62），moderate （n=56）and severe（n=36） groups. The bone mineral density （BMD）in lumbar vertebrate and femoral bone was measured by Dual-energy X-ray absorptiometry（ DEXA）made in America．The clinical factors and biochemical parameters of patients in different groups were observed and the correlation between lower extremity atherosclerosis and bone mineral density．Results（1） With the increase in the severity of LEA，the HBA1c，FBG， 2HPBG， TC， TG and LDL were gradually increased ，but HDL was decreased in the four groups， there was significant differences（P＜0.05）.（2）The severity score of LEA was negative correlated with the BMD in lumbar vertebrat and femoral bone.（3）Multivariate Logistic regression analysis showed the change of BMD，HBA1c and TC were major risk factors of LEA T2-DM .Conclusions With the increase in the severity of LEA in T2-DM，the decrease of BMD is more obvious．The decrease of BMD is a risk factor of LEA production and development．
2012, 33(02)
Abstract:
［Abstract］Objective To explore the application value of percutaneous kyphoplasty（PKP）in the treatment of osteoporotic vertebral compression fractures（OVCF）and the prevention of complications．Methods From June 2006 to January 2009，a totally of 97 vertebra with OVCF in 68 patients were treated by using domestic PKP tool system．There were 21 males and 47 females，with the age of 62～82 years．The puncture point was located between costal fovea of the transverse process and superior articular process for thoracic vertebra，and in lateral upper edge of the pedicle projection for lumbar vertebra．With the patients in prone position，the puncture needle was inserted into the fractured vertebral body via the lateral edge of pedicle under C-arm guidance．After the location of neddle tip was confirmed，the sugery was carried out according to the operative procedure．Results The operations were successful in all patients．The bone cement leaked into intervertebral disc in 2 cases，paravertebral space in 2 cases and spinal canal in 4 cases of 7 vertebra. The incidence of complication was 11.76%．1 case had got a pain in the anterolateral thigh after ambulation on the second postoperative day，which was relieved by symptomatic treatment 6 days later．2 cases which suffered from decreased blood pressure and weak pulse during the operation，were improved after treatment. Postoperative fever was found in 3 cases．All patients began to ambulate on the second day after operation and were followed up for 3-18 months．The average height of severe collapsed vertebra is increased from 1.58cm to 2.13cm．Compared with the preoperative measurement，Cobb angle was reduced 12.3 ° ± 3.4 on average．Conclusions The PKP in the treatment of OVCF can quickly relieve pain，recover the height of fractured vertebra，improve kyphosis and life quality．The measurement of needle angle，observation of vertebra with MRI and 3DCT，monitoring of bone cement injection whole process can effectively reduce the incidence of complications.
［Abstract］Objective To explore the application value of percutaneous kyphoplasty（PKP）in the treatment of osteoporotic vertebral compression fractures（OVCF）and the prevention of complications．Methods From June 2006 to January 2009，a totally of 97 vertebra with OVCF in 68 patients were treated by using domestic PKP tool system．There were 21 males and 47 females，with the age of 62～82 years．The puncture point was located between costal fovea of the transverse process and superior articular process for thoracic vertebra，and in lateral upper edge of the pedicle projection for lumbar vertebra．With the patients in prone position，the puncture needle was inserted into the fractured vertebral body via the lateral edge of pedicle under C-arm guidance．After the location of neddle tip was confirmed，the sugery was carried out according to the operative procedure．Results The operations were successful in all patients．The bone cement leaked into intervertebral disc in 2 cases，paravertebral space in 2 cases and spinal canal in 4 cases of 7 vertebra. The incidence of complication was 11.76%．1 case had got a pain in the anterolateral thigh after ambulation on the second postoperative day，which was relieved by symptomatic treatment 6 days later．2 cases which suffered from decreased blood pressure and weak pulse during the operation，were improved after treatment. Postoperative fever was found in 3 cases．All patients began to ambulate on the second day after operation and were followed up for 3-18 months．The average height of severe collapsed vertebra is increased from 1.58cm to 2.13cm．Compared with the preoperative measurement，Cobb angle was reduced 12.3 ° ± 3.4 on average．Conclusions The PKP in the treatment of OVCF can quickly relieve pain，recover the height of fractured vertebra，improve kyphosis and life quality．The measurement of needle angle，observation of vertebra with MRI and 3DCT，monitoring of bone cement injection whole process can effectively reduce the incidence of complications.
2012, 33(02)
Abstract:
［Abstract］Objective To summarize the experience in the treatment of elderly patients（65 years of age）with lung cancer by using video-assisted thoracic surgery（VATS）．Methods 72 elderly patients with lung cancer were enrolled in this study．36 cases of lung cancer in elderly patients were treated with VATS，including lung wedge resection in 20 cases，lung resection in 11 cases，transit thoracotomy in 5 cases，the intraoperative frozen tissues were given fast pathological examination， and systematic lymph node dissection was performed in parallel．Another 36 patients were given traditional surgery．Results All patients got complete tumor resection，the postoperative pathology report showed no cancer cells in the incisal margin of tumors．There were no perioperative deaths and no serious postoperative complications（12.5±1.4）right chest lymph nodes and（10.5 ±0.5）left chest lymph nodes were dissected，respectively．The time of VATS was 35 ~ 114 min，mean（77±13）min．Mean postoperative hospital stay time was（5.9±2.5）d，drainage tube indwelling time was（3.4±1.6）d．One patients with a history of heart disease stayed in ICU for 3 days．Conclusion Comapred with traditional thoracotomy， VATS for thoracic surgery in elderly patients has minimal invasion，fewer complications，little physical interference，rapid postoperative recovery，and is safer especially for patients with chronic heart and lung diseases
［Abstract］Objective To summarize the experience in the treatment of elderly patients（65 years of age）with lung cancer by using video-assisted thoracic surgery（VATS）．Methods 72 elderly patients with lung cancer were enrolled in this study．36 cases of lung cancer in elderly patients were treated with VATS，including lung wedge resection in 20 cases，lung resection in 11 cases，transit thoracotomy in 5 cases，the intraoperative frozen tissues were given fast pathological examination， and systematic lymph node dissection was performed in parallel．Another 36 patients were given traditional surgery．Results All patients got complete tumor resection，the postoperative pathology report showed no cancer cells in the incisal margin of tumors．There were no perioperative deaths and no serious postoperative complications（12.5±1.4）right chest lymph nodes and（10.5 ±0.5）left chest lymph nodes were dissected，respectively．The time of VATS was 35 ~ 114 min，mean（77±13）min．Mean postoperative hospital stay time was（5.9±2.5）d，drainage tube indwelling time was（3.4±1.6）d．One patients with a history of heart disease stayed in ICU for 3 days．Conclusion Comapred with traditional thoracotomy， VATS for thoracic surgery in elderly patients has minimal invasion，fewer complications，little physical interference，rapid postoperative recovery，and is safer especially for patients with chronic heart and lung diseases
2012, 33(02)
Abstract:
［Abstract］Objective To evaluate the therapeutic effect of Pingyangmycin and lipiodol emulsion（PLE） embolization for cavernous hemangioma of liver（CHL）．Methods We analyzed the clinical data of the 42 cases of the CHL treated by selectively injecting pingyangmycin lipiodol emulsion（PLE）into the supplying artery of tumors，and the patients were followed up after the operation for 6 months to 12 months．Results All the 42 patients were performed successfully with Hepatic artery intubation and 17 of these patients were performed with super selectively sent into the supplying artery of tumors．The embolism times was(1.3±1.1)/person for 5～10 cm CHL，(2.8±2.2)/person for 10～20 cm and (3.3±2.3)/person for 21～30 cm．Followed up 6 months to 18 months，CHL reduced by over 50％ in 4 cases，and reduced by 25％~50％ in 31 cases，and reduced less than 25% in 6 cases，respectively．But one case didn't appear clinical effect． There were no serious complications．Conclusion It is reliable and feasible that treating CHL with PLE through super selective hepatic artery intubation
［Abstract］Objective To evaluate the therapeutic effect of Pingyangmycin and lipiodol emulsion（PLE） embolization for cavernous hemangioma of liver（CHL）．Methods We analyzed the clinical data of the 42 cases of the CHL treated by selectively injecting pingyangmycin lipiodol emulsion（PLE）into the supplying artery of tumors，and the patients were followed up after the operation for 6 months to 12 months．Results All the 42 patients were performed successfully with Hepatic artery intubation and 17 of these patients were performed with super selectively sent into the supplying artery of tumors．The embolism times was(1.3±1.1)/person for 5～10 cm CHL，(2.8±2.2)/person for 10～20 cm and (3.3±2.3)/person for 21～30 cm．Followed up 6 months to 18 months，CHL reduced by over 50％ in 4 cases，and reduced by 25％~50％ in 31 cases，and reduced less than 25% in 6 cases，respectively．But one case didn't appear clinical effect． There were no serious complications．Conclusion It is reliable and feasible that treating CHL with PLE through super selective hepatic artery intubation
2012, 33(02)
Abstract:
［Abstract］Objective To compare the clinical result of different type of channel of percutaneous renal fistula in treatment of complex upper urinary calculi．Methods 32 patients accepted the operation with 16 F channel of percutaneous renal fistula，and 20 patients with 20 F channel．After the channel was made，the litholapaxy was performed with the help of Holmium Laser．The clean rate of the stone in one-stage operation，average time of the operation，the postoperative decreasing level of hemoglobin and the rate of blood transfusion were compared between these 2 groups．Results The clean rate of the stone in one-stage operation of the 16F group was 47%（15/32）， and to the 20F group，it was 65%（13/20），the difference was insignificant（P＞0.05）；the average time of operation of 16F group was 2.3±0.6 hours，and it was 1.5±0.5 hours in 20 F group，the difference was significant（P＜0.05）； the postoperative decreasing level of hemoglobin of 16 F group was 16±7.5 g/L，and it was 18±8.5 g/L in 20 F group， and the difference was insignificant（P＞0.05）；the rate of blood transfusion of 16F group was 6%（2/32）， and it was 10%（2/20）to 20 F group，the difference was insignificant（P＞0.05）．Conclusion In the treatment of complex upper urinary calculi， when compared with 16F channel of percutaneous renal fistula， 20 F channel is more effective，rapid and safe，so this channel deserves to be used in clinical works
［Abstract］Objective To compare the clinical result of different type of channel of percutaneous renal fistula in treatment of complex upper urinary calculi．Methods 32 patients accepted the operation with 16 F channel of percutaneous renal fistula，and 20 patients with 20 F channel．After the channel was made，the litholapaxy was performed with the help of Holmium Laser．The clean rate of the stone in one-stage operation，average time of the operation，the postoperative decreasing level of hemoglobin and the rate of blood transfusion were compared between these 2 groups．Results The clean rate of the stone in one-stage operation of the 16F group was 47%（15/32）， and to the 20F group，it was 65%（13/20），the difference was insignificant（P＞0.05）；the average time of operation of 16F group was 2.3±0.6 hours，and it was 1.5±0.5 hours in 20 F group，the difference was significant（P＜0.05）； the postoperative decreasing level of hemoglobin of 16 F group was 16±7.5 g/L，and it was 18±8.5 g/L in 20 F group， and the difference was insignificant（P＞0.05）；the rate of blood transfusion of 16F group was 6%（2/32）， and it was 10%（2/20）to 20 F group，the difference was insignificant（P＞0.05）．Conclusion In the treatment of complex upper urinary calculi， when compared with 16F channel of percutaneous renal fistula， 20 F channel is more effective，rapid and safe，so this channel deserves to be used in clinical works
2012, 33(02)
Abstract:
［Abstract］Objective To study the genetic characteristics and genetic variation of the nonstructural protein NSP4 of Human rotavirus strains．Methods We selected twelve positive RV samples among 96 infants with diarrhea who were hospitalized in the Department of Peadiatrics，the First Affiliated Hospital of Kunming Medical University from October to December in 2007 and 2008．NSP4 genes was amplified by RT-PCR and then cDNAs were sequenced. The homology of the nucleotide sequences of the NSP4 genes were analyzed by DNA assist and Clustal-mp．Results（1）NSP4 gene was amplifie from 12（6 of 2007 and 6 of 2008）RV positive samples. Among the twelve Kunming strains， the nucleotide homologies of the ten Wa strains to the four HRV standard strains （Wa，KUN，AU-1 and Hochi）and three animal RV（ EW，OSU and All）were 96.3%，85.3%，84.7%， 95.1% and 57.7%，92%，82.8%．Compared with the epidemic strains in other different areas of China， the nucleotide homology of the NSP4 gene was over 95%．Compared with the standard strains from the above GenBank Database，the nucleotide homology of the two KUN strain were 85.3%，98.2%，82.8%，85.9% and 60.1%， 85.3%，94.3%．And compared with the epidemic strains other different areas of China， the nucleotide homology of the NSP4 gene was about 85%.（2）6 unique conserved amino acids of 10 Wa strains differing from Wa at aa34， aa76，aa141，aa161 and aa169 were identified within NSP4; 2 unique conserved amino acids of 2 KUN strains differing from KUN at aa87 and aa140 were identified within NSP4．Conclusions Among the NSP4 of the twelve Kunming strains，the genotypes are Wa strain and KUN strain．The most dominant genotype of NSP4 is Wa strain
［Abstract］Objective To study the genetic characteristics and genetic variation of the nonstructural protein NSP4 of Human rotavirus strains．Methods We selected twelve positive RV samples among 96 infants with diarrhea who were hospitalized in the Department of Peadiatrics，the First Affiliated Hospital of Kunming Medical University from October to December in 2007 and 2008．NSP4 genes was amplified by RT-PCR and then cDNAs were sequenced. The homology of the nucleotide sequences of the NSP4 genes were analyzed by DNA assist and Clustal-mp．Results（1）NSP4 gene was amplifie from 12（6 of 2007 and 6 of 2008）RV positive samples. Among the twelve Kunming strains， the nucleotide homologies of the ten Wa strains to the four HRV standard strains （Wa，KUN，AU-1 and Hochi）and three animal RV（ EW，OSU and All）were 96.3%，85.3%，84.7%， 95.1% and 57.7%，92%，82.8%．Compared with the epidemic strains in other different areas of China， the nucleotide homology of the NSP4 gene was over 95%．Compared with the standard strains from the above GenBank Database，the nucleotide homology of the two KUN strain were 85.3%，98.2%，82.8%，85.9% and 60.1%， 85.3%，94.3%．And compared with the epidemic strains other different areas of China， the nucleotide homology of the NSP4 gene was about 85%.（2）6 unique conserved amino acids of 10 Wa strains differing from Wa at aa34， aa76，aa141，aa161 and aa169 were identified within NSP4; 2 unique conserved amino acids of 2 KUN strains differing from KUN at aa87 and aa140 were identified within NSP4．Conclusions Among the NSP4 of the twelve Kunming strains，the genotypes are Wa strain and KUN strain．The most dominant genotype of NSP4 is Wa strain
2012, 33(02)
Abstract:
［Abstract］Objective To observe the morphology of symphysial surface in male Han people in Yunnan， and provide a evidence for finding a age estimation formula suitable to people in South of China．Method The morphology of symphysial surface in male Han people in Yunnan was observed．Results The pubic symphysis ditch and ridge of Yunnan Han male people appeared 1 to 2 years earlier than literatures reported， the appearance rate of ossifying nodules was not high．The domestic literature data showed that the ventral margin of emergence appeared at around age 27，fully formed to 35 years and gradually widened，there was no uplift reports，male pubic appeared uplift．The ventral surface changes appeared 1～2 years ealier than those reported in literature．Conclusion The relation between morphological change of symphysial surface and age has certain particularity in Yunnan male Han people，some indexes appear slightly earlier than domestic other people，the appearance rate of some indexes is not high
［Abstract］Objective To observe the morphology of symphysial surface in male Han people in Yunnan， and provide a evidence for finding a age estimation formula suitable to people in South of China．Method The morphology of symphysial surface in male Han people in Yunnan was observed．Results The pubic symphysis ditch and ridge of Yunnan Han male people appeared 1 to 2 years earlier than literatures reported， the appearance rate of ossifying nodules was not high．The domestic literature data showed that the ventral margin of emergence appeared at around age 27，fully formed to 35 years and gradually widened，there was no uplift reports，male pubic appeared uplift．The ventral surface changes appeared 1～2 years ealier than those reported in literature．Conclusion The relation between morphological change of symphysial surface and age has certain particularity in Yunnan male Han people，some indexes appear slightly earlier than domestic other people，the appearance rate of some indexes is not high
2012, 33(02)
Abstract:
［Abstract］Objective To investigate the prevalence and distribution of periodontopathogens in the subgingival plaque of patients with coronary heart disease（CHD）and periodontitis．Methods 50 subgingival plaque samples of patients with CP and 50 subgingival plaque samples of patients with CP and CHD were collected and their bacteria DNA was extracted．Six periodontal pathogens including Porphyromonas gingivalis（Pg）， Prevotella intermedia（Pi），Fusobacterium nucleatum（Fn），Actinobacillus actinomycetemcomitans（Aa）， Tannerella forsythensis（Tf）and Treponema denticola（Td） were detected by the Nested polymerase chain reaction（Nested PCR）．Results In the CP group，the rates of periodontal bacteria were Pg 98%，Pi 92%，Fn 88%，Aa 6%，Tf 92% and Td 98%；In the CP combined with CHD group ，the rates of periodontal bacteria were Pg 100%，Pi 94%，Fn 80%，Aa 14%，Tf 96% and Td 98%．Conclusion The distribution of putative periodontopathogens Pg，Pi，Fn，Aa，Tf and Td is similar in the CP group and CP combined with CHD group
［Abstract］Objective To investigate the prevalence and distribution of periodontopathogens in the subgingival plaque of patients with coronary heart disease（CHD）and periodontitis．Methods 50 subgingival plaque samples of patients with CP and 50 subgingival plaque samples of patients with CP and CHD were collected and their bacteria DNA was extracted．Six periodontal pathogens including Porphyromonas gingivalis（Pg）， Prevotella intermedia（Pi），Fusobacterium nucleatum（Fn），Actinobacillus actinomycetemcomitans（Aa）， Tannerella forsythensis（Tf）and Treponema denticola（Td） were detected by the Nested polymerase chain reaction（Nested PCR）．Results In the CP group，the rates of periodontal bacteria were Pg 98%，Pi 92%，Fn 88%，Aa 6%，Tf 92% and Td 98%；In the CP combined with CHD group ，the rates of periodontal bacteria were Pg 100%，Pi 94%，Fn 80%，Aa 14%，Tf 96% and Td 98%．Conclusion The distribution of putative periodontopathogens Pg，Pi，Fn，Aa，Tf and Td is similar in the CP group and CP combined with CHD group
2012, 33(02)
Abstract:
［Abstract］Objective To observe the effect of esculin and digitalisglycosides eye drops combined with ciliary exercise training on accommodative asthenopia．Methods Forty patients with asthenopia were included in the study．patients were given esculin and digitalisglycosides eye drops and ciliary exercise training based on refraction correction．Results Asthenopia was imparoved significantly after treatment，the difference is significant（P＜0.05）．There was no significant difference in accommodation（P＞0.05）．Conclusion Esculin and digitalisglycosides eye drops combined with ciliary exercise training could obviously relieve asthenopia.
［Abstract］Objective To observe the effect of esculin and digitalisglycosides eye drops combined with ciliary exercise training on accommodative asthenopia．Methods Forty patients with asthenopia were included in the study．patients were given esculin and digitalisglycosides eye drops and ciliary exercise training based on refraction correction．Results Asthenopia was imparoved significantly after treatment，the difference is significant（P＜0.05）．There was no significant difference in accommodation（P＞0.05）．Conclusion Esculin and digitalisglycosides eye drops combined with ciliary exercise training could obviously relieve asthenopia.
2012, 33(02)
Abstract:
［Abstract］Objective To assess the clinical efficacy of Uterine Arterial Embolization（UAE）in the treatment adenomyosis．Method Twenty-eight patients with adenomyosis were treated by UAE，and the the pain rating of uterus，amount of menstruation，menorrhagia and volume change of uterus focus were observed after treatment．Results Compared with those before treatment，the pain rating increased，the amount of menstruation reduced，uterine volume reduced after treatment，the differences were statistically signifficant（P＜0.05）．Conclusion UAE is an effective and safe method in the treatment of adenomyosis
［Abstract］Objective To assess the clinical efficacy of Uterine Arterial Embolization（UAE）in the treatment adenomyosis．Method Twenty-eight patients with adenomyosis were treated by UAE，and the the pain rating of uterus，amount of menstruation，menorrhagia and volume change of uterus focus were observed after treatment．Results Compared with those before treatment，the pain rating increased，the amount of menstruation reduced，uterine volume reduced after treatment，the differences were statistically signifficant（P＜0.05）．Conclusion UAE is an effective and safe method in the treatment of adenomyosis
2012, 33(02)
Abstract:
［Abstract］Objective To investigate the influencing factors of prognosis of osteosarcoma in children and adolescence．Methods We retrospectively analyzed the clinical data of 32 adolescent patients with osteosarcoma admitted in our hospital from January 1990 to January 2006．All adolescent patients with osteosarcoma were given COSS 96 treatment protocol．The 5 a overall survival（OS）rate and event-free survival（EFS）rate were analyzed and their relation with metastasis，surgery types，the location of osteosarcoma，histological types，reactions to preoperative chemotherapy，gender and age of patients，were also analyzed．Results The 5 a OS and EFS was 59.4%（19/32）and 46.9%（15/32），respectively．The 5 a OS of patients without metastasis was significantly higher than that of patients with metastasis（P＜0.05），and was significantly higher in patients with late metastasis than early metastasis（P＜0.05）．There was no significant difference in the recurrence rate，5 a OS and 5 a EFS between Limb salvage surgery and amputation surgery（P＞0.05）．There was also no significant difference in 5 a OS and 5 a EFS between different location of osteosarcoma and different histological types（P＞0.05）．The 5 a OS and 5 a EFS of patients sensitive to new chemotherapy were significantly higher than those of patients insensitive to new chemotherapy（P＜0.05）．There was no significant difference in 5 a OS and 5 a EFS between patients with different gender（P＞0.05）．The 5 a OS and EFS of patients younger than 14 were significantly higher than those of patients over 14［75%（12/16）and 68.8%（11/16）vs. 43.8%（7/16）and 26.7%（4/16），P＜0.05］．Conclusions Most adolescent patients with osteosarcoma can get satisfying therapeutic effect by enhanced chemotherapy and surgery．Limb salvage surgery can improve the life quality of patients，while does not increase the risk of poor prognosis．The prognosis is poor in patients with metastasis and local recurrence， while the prognosis is good in patients with good reaction to chemotherapy and younger than 14 years
［Abstract］Objective To investigate the influencing factors of prognosis of osteosarcoma in children and adolescence．Methods We retrospectively analyzed the clinical data of 32 adolescent patients with osteosarcoma admitted in our hospital from January 1990 to January 2006．All adolescent patients with osteosarcoma were given COSS 96 treatment protocol．The 5 a overall survival（OS）rate and event-free survival（EFS）rate were analyzed and their relation with metastasis，surgery types，the location of osteosarcoma，histological types，reactions to preoperative chemotherapy，gender and age of patients，were also analyzed．Results The 5 a OS and EFS was 59.4%（19/32）and 46.9%（15/32），respectively．The 5 a OS of patients without metastasis was significantly higher than that of patients with metastasis（P＜0.05），and was significantly higher in patients with late metastasis than early metastasis（P＜0.05）．There was no significant difference in the recurrence rate，5 a OS and 5 a EFS between Limb salvage surgery and amputation surgery（P＞0.05）．There was also no significant difference in 5 a OS and 5 a EFS between different location of osteosarcoma and different histological types（P＞0.05）．The 5 a OS and 5 a EFS of patients sensitive to new chemotherapy were significantly higher than those of patients insensitive to new chemotherapy（P＜0.05）．There was no significant difference in 5 a OS and 5 a EFS between patients with different gender（P＞0.05）．The 5 a OS and EFS of patients younger than 14 were significantly higher than those of patients over 14［75%（12/16）and 68.8%（11/16）vs. 43.8%（7/16）and 26.7%（4/16），P＜0.05］．Conclusions Most adolescent patients with osteosarcoma can get satisfying therapeutic effect by enhanced chemotherapy and surgery．Limb salvage surgery can improve the life quality of patients，while does not increase the risk of poor prognosis．The prognosis is poor in patients with metastasis and local recurrence， while the prognosis is good in patients with good reaction to chemotherapy and younger than 14 years
2012, 33(02)
Abstract:
［Abstract］Objective To evaluate clinical value of colposcopy in diagnosis of cervical lesions．Method The colposcopy images and histologic findings were analyzed and summarized in 217 cases with suspicious cervical lesions．Results Colposcopy images found 68 cases of suspicious CIN，and 51 cases of CIN were pathologically confirmed．4 cases of condyloma were diagnosed by colposopy and confirmed by pathological result．2 cases of cervical cancer were diagnosed by colposcopic diagnosis and pathologically confirmed．145 cases of cervical inflamation were diagnosed by colposopy and 142 cases were pathologically confirmed．Conclusion Colposcopy has a significant value in detection of cervical precancerous lesion and early cervical cancer
［Abstract］Objective To evaluate clinical value of colposcopy in diagnosis of cervical lesions．Method The colposcopy images and histologic findings were analyzed and summarized in 217 cases with suspicious cervical lesions．Results Colposcopy images found 68 cases of suspicious CIN，and 51 cases of CIN were pathologically confirmed．4 cases of condyloma were diagnosed by colposopy and confirmed by pathological result．2 cases of cervical cancer were diagnosed by colposcopic diagnosis and pathologically confirmed．145 cases of cervical inflamation were diagnosed by colposopy and 142 cases were pathologically confirmed．Conclusion Colposcopy has a significant value in detection of cervical precancerous lesion and early cervical cancer
2012, 33(02)
Abstract:
［Abstract］Objective To explore the clinical value of high-frequency color ultrasonography in evaluating the vascular endothelium function in type 2 diabetes patients．Methods The general conditions and biochemical indexes of 77 type 2 diabetes patients and 36 normal control subjects were measured．Right brachial artery flow was detected for the flow mediated dilation（FMD）and endothelium independent dilation（EID）after taking nitroglycerin in the two groups with high-frequency color ultrasonography．Results The age，total cholesterol and low density lipoprotein had no significant difference between two groups，but body mass index，fasting blood-glucose and triglyceride in T2DM group were significantly higher than those in control group whereas high density lipoprotein was significantly low in T2DM group．In T2DM group，FMD was significantly decreased compared with that in normal control group（P＜0.01］，but NID after taking nitroglycerin orally had no significant difference between two groups（P＞0.05）．Conclusion High-frequency color ultrasonography has a high clinical diagnositic value in evaluating vascular endothelium function in type 2 diabetes patients
［Abstract］Objective To explore the clinical value of high-frequency color ultrasonography in evaluating the vascular endothelium function in type 2 diabetes patients．Methods The general conditions and biochemical indexes of 77 type 2 diabetes patients and 36 normal control subjects were measured．Right brachial artery flow was detected for the flow mediated dilation（FMD）and endothelium independent dilation（EID）after taking nitroglycerin in the two groups with high-frequency color ultrasonography．Results The age，total cholesterol and low density lipoprotein had no significant difference between two groups，but body mass index，fasting blood-glucose and triglyceride in T2DM group were significantly higher than those in control group whereas high density lipoprotein was significantly low in T2DM group．In T2DM group，FMD was significantly decreased compared with that in normal control group（P＜0.01］，but NID after taking nitroglycerin orally had no significant difference between two groups（P＞0.05）．Conclusion High-frequency color ultrasonography has a high clinical diagnositic value in evaluating vascular endothelium function in type 2 diabetes patients
2012, 33(02)
Abstract:
［Abstract］Objective To investigate the effect of combined I125-seed implantation and gimeracil and oteracil capsules adjuvant chemotherapy on head and neck cancer．Methods From Octorber 2008 to Octorber 2010，40 patients with head or neck cancer were selected in our hospital．Pathological types of the head or neck cancer cases were：26 cases of squamous cell carcinoma and 14 cases of poorly differentiated adenocarcinoma According to anatomical parts，the head or neck cancer cases were divided into：7 cases with hypopharyngeal Carcinoma，5 cases with maxillary sinus Carcinoma，4 cases with oral cancer；4 cases with cervical metastatic carcinoma．All patients could not receive surgery due to systemic disease or locally advanced cancer，and they were given a I125-seed implantation，the matched peripheral dose was 90～110 Gy．All patients were given two - cycle of postoperative adjuvant chemotherapy with 80 mg/m2 of gimeracil and oteracil capsules．The patients were followed up，and the acute and chronic radiation injury， local control and survival of patients were analyzed．Results In patients with cancer，complete remission was found in 13 cases（65%），partial remission in 5 cases（25%）， invalid in 2 cases（10%），and the effective rate was 90%．Postoperative follow-up for 42 months，1 case was lost in the follow-up，the 1- year，2 - year and 3-year survival rate was 80.0%，48.75% and 13.0%， respectively. The median survival period was 16 months. Primary complications were oral mucositis and local hemorrhage. Conclusion I125-seed implantation combined with gimeracil and oteracil capsules adjuvant chemotherapy has good therapeutic effect on head and neck cancer with surgical resection taboo，with satisfying survival rate in patients and fewer complications
［Abstract］Objective To investigate the effect of combined I125-seed implantation and gimeracil and oteracil capsules adjuvant chemotherapy on head and neck cancer．Methods From Octorber 2008 to Octorber 2010，40 patients with head or neck cancer were selected in our hospital．Pathological types of the head or neck cancer cases were：26 cases of squamous cell carcinoma and 14 cases of poorly differentiated adenocarcinoma According to anatomical parts，the head or neck cancer cases were divided into：7 cases with hypopharyngeal Carcinoma，5 cases with maxillary sinus Carcinoma，4 cases with oral cancer；4 cases with cervical metastatic carcinoma．All patients could not receive surgery due to systemic disease or locally advanced cancer，and they were given a I125-seed implantation，the matched peripheral dose was 90～110 Gy．All patients were given two - cycle of postoperative adjuvant chemotherapy with 80 mg/m2 of gimeracil and oteracil capsules．The patients were followed up，and the acute and chronic radiation injury， local control and survival of patients were analyzed．Results In patients with cancer，complete remission was found in 13 cases（65%），partial remission in 5 cases（25%）， invalid in 2 cases（10%），and the effective rate was 90%．Postoperative follow-up for 42 months，1 case was lost in the follow-up，the 1- year，2 - year and 3-year survival rate was 80.0%，48.75% and 13.0%， respectively. The median survival period was 16 months. Primary complications were oral mucositis and local hemorrhage. Conclusion I125-seed implantation combined with gimeracil and oteracil capsules adjuvant chemotherapy has good therapeutic effect on head and neck cancer with surgical resection taboo，with satisfying survival rate in patients and fewer complications
2012, 33(02)
Abstract:
［Abstract］Objective To explore the hepatitis B virus surface antigen（HBsAg）screening and infection of hepatitis B virus（HBV）among puerperants giving birth to children in hospitals in different areas of Yunnan． Methods We selected three cities by quota sampling．Data about HBsAg screening and infection of HBV among puerperants were reported to local hospitals from January to June of 2011，the data in the three cities were collected and analyzed．Results The gold immunochromatography assay（GICA）was used by most hospitals for HBsAg screening ，a total of 68753 puerperants were reported by the 332 hospitals and the HBsAg screening rate was 97.44 %.The consistent rate of screening was 98.83 %. The positive rate in those who had screened for HBsAg was 2.35% and the positive rate among three areas showed significant difference（P＜0.01）．The constituent ratio of both HBsAg-and HBeAg -positive puerperants in villages was higher than that in cities（P＜0.05）．The constituent ratio of both HBsAg-and HBeAg-positive puerperants in 15～29 year-old group was higher than that in 30～42 year-old group（P＜0.01）．Conclusions The antepartum HBsAg screening rate is high among those who gave birth to children in hospitals．GICA is mainly used to do HBsAg screening in health centers in different area of Yunnan during the project．Although screening results are credible，the undetected rate exists．So HBsAg screening and retesting with sensitive，stable and mature ELISA method is a better choice
［Abstract］Objective To explore the hepatitis B virus surface antigen（HBsAg）screening and infection of hepatitis B virus（HBV）among puerperants giving birth to children in hospitals in different areas of Yunnan． Methods We selected three cities by quota sampling．Data about HBsAg screening and infection of HBV among puerperants were reported to local hospitals from January to June of 2011，the data in the three cities were collected and analyzed．Results The gold immunochromatography assay（GICA）was used by most hospitals for HBsAg screening ，a total of 68753 puerperants were reported by the 332 hospitals and the HBsAg screening rate was 97.44 %.The consistent rate of screening was 98.83 %. The positive rate in those who had screened for HBsAg was 2.35% and the positive rate among three areas showed significant difference（P＜0.01）．The constituent ratio of both HBsAg-and HBeAg -positive puerperants in villages was higher than that in cities（P＜0.05）．The constituent ratio of both HBsAg-and HBeAg-positive puerperants in 15～29 year-old group was higher than that in 30～42 year-old group（P＜0.01）．Conclusions The antepartum HBsAg screening rate is high among those who gave birth to children in hospitals．GICA is mainly used to do HBsAg screening in health centers in different area of Yunnan during the project．Although screening results are credible，the undetected rate exists．So HBsAg screening and retesting with sensitive，stable and mature ELISA method is a better choice
2012, 33(02)
Abstract:
［Abstract］Objective To explore the relationship between the changes of plasma N-terminal pro-B-type natriuretic peptide（NT-proBNP）and left ventricular remodeling（LVRM）in early acute myocardial infarction（AMI）patients before and after PCI．Methods Seventy-two patients admitted to our hospital with AMI were randomly divided into two groups，35 were in medicine treated group（M-group）and another 37 were in the medicine plus emergent PCI group（PCI-group）．The plasma NT-proBNP was measured within 24 hours after admission or before PCI and on the 10 th day after admission， and echocardiogram were performed for all of the patients at admission and on the 9th or 10th day after admission to evaluate the left ventricular remolding by measuring the left ventricular end-diastolic dimension （LVEDD）， left ventricular ejection fraction（LVEF），left ventricular shortening（FS）and left ventricular end-diastolic volume（LVEDV）to analyze the relationship between the NT-proBNP and the morphology and function of the left ventricle．Results The levels of plasma NT-proBNP decreased，LVEF and FS increased after the treatment in both of the two groups than that before treatment significantly（P＜0.05），and no significant difference were observed for LVEDD and LVEDV（P＞0.05）．No significant difference was observed for the plasma NT-proBNP，LVEF，FS，LVEDD，LVEDV between the two groups before the treatment（P＞0.05）．The levels of plasma NT-proBNP decreased and LVEF， FS increased significantly after the different treatment in PCI-group than that of M-group（P＜0.05），but LVEDD，LVEDV decreased insignificantly（P＞0.05）．Conclusions The increased plasma NT-proBNP in AMI patients may reflect the left ventricular remodeling．PCI may decrease significantly the plasma NT-proBNP，imorove the left ventricular function and reduce the incidence of left ventricular remodeling
［Abstract］Objective To explore the relationship between the changes of plasma N-terminal pro-B-type natriuretic peptide（NT-proBNP）and left ventricular remodeling（LVRM）in early acute myocardial infarction（AMI）patients before and after PCI．Methods Seventy-two patients admitted to our hospital with AMI were randomly divided into two groups，35 were in medicine treated group（M-group）and another 37 were in the medicine plus emergent PCI group（PCI-group）．The plasma NT-proBNP was measured within 24 hours after admission or before PCI and on the 10 th day after admission， and echocardiogram were performed for all of the patients at admission and on the 9th or 10th day after admission to evaluate the left ventricular remolding by measuring the left ventricular end-diastolic dimension （LVEDD）， left ventricular ejection fraction（LVEF），left ventricular shortening（FS）and left ventricular end-diastolic volume（LVEDV）to analyze the relationship between the NT-proBNP and the morphology and function of the left ventricle．Results The levels of plasma NT-proBNP decreased，LVEF and FS increased after the treatment in both of the two groups than that before treatment significantly（P＜0.05），and no significant difference were observed for LVEDD and LVEDV（P＞0.05）．No significant difference was observed for the plasma NT-proBNP，LVEF，FS，LVEDD，LVEDV between the two groups before the treatment（P＞0.05）．The levels of plasma NT-proBNP decreased and LVEF， FS increased significantly after the different treatment in PCI-group than that of M-group（P＜0.05），but LVEDD，LVEDV decreased insignificantly（P＞0.05）．Conclusions The increased plasma NT-proBNP in AMI patients may reflect the left ventricular remodeling．PCI may decrease significantly the plasma NT-proBNP，imorove the left ventricular function and reduce the incidence of left ventricular remodeling
2012, 33(02)
Abstract:
［Abstract］Objective To compare the effects of etomidate and propofol anesthesia induction on cardiovascular stability in elderly patients，and to guide drugs use in the anesthesia induction in elderly patients. Methods 60 elderly patients were randomly divided into two groups：acupuncture group etomidate（A），propofol needle group（B），induced by 0.2 mg/kg of midazolam maleate tallet．Patients in A group were given 0.3 mg/kg of etomidate，and patients in B group were given 1.5 mg/kg of propofol-pin. All patients were also intravenously injected 3 μg/kg of Norcuron and 0.1 mg/kg of Fentanyl．HR，SBP，DBP and MAP of patients were recorded before induction，1 min，2 min and 5 min after induction．The adverse reactions including nausea，vomiting， intravenous pain，involuntary muscle activity and allergic reactions were also observed．Results Compared with baseline values before induction of anesthesia，HR，SBP，DBP and MAP of patients in both groups were decreased in different degrees after induction of anesthesia．The decrease was statistically significant at 1 min and 5 min after induction of anesthesia（P＜0.5）．Compared with B group，The decrease of SBP and MAP was more obvious in A group at 2 mins and 5 mins after induction of anesthesia（P＜0.5）. There were no nausea， vomiting and allergic reactions in two groups．There was intravenous injection pain in B group and involuntary muscle activity in A group，there was a significant difference（P ＜0.05）．Conclusion Both etomidate and propofol inhibit the cardiovascular stability in elderly patients in different degrees，but etomidate has less effect on the circulation of patients and vascular stability，so etomidate is more suitable as the preferred intravenous anesthetic induction drug for elderly patients
［Abstract］Objective To compare the effects of etomidate and propofol anesthesia induction on cardiovascular stability in elderly patients，and to guide drugs use in the anesthesia induction in elderly patients. Methods 60 elderly patients were randomly divided into two groups：acupuncture group etomidate（A），propofol needle group（B），induced by 0.2 mg/kg of midazolam maleate tallet．Patients in A group were given 0.3 mg/kg of etomidate，and patients in B group were given 1.5 mg/kg of propofol-pin. All patients were also intravenously injected 3 μg/kg of Norcuron and 0.1 mg/kg of Fentanyl．HR，SBP，DBP and MAP of patients were recorded before induction，1 min，2 min and 5 min after induction．The adverse reactions including nausea，vomiting， intravenous pain，involuntary muscle activity and allergic reactions were also observed．Results Compared with baseline values before induction of anesthesia，HR，SBP，DBP and MAP of patients in both groups were decreased in different degrees after induction of anesthesia．The decrease was statistically significant at 1 min and 5 min after induction of anesthesia（P＜0.5）．Compared with B group，The decrease of SBP and MAP was more obvious in A group at 2 mins and 5 mins after induction of anesthesia（P＜0.5）. There were no nausea， vomiting and allergic reactions in two groups．There was intravenous injection pain in B group and involuntary muscle activity in A group，there was a significant difference（P ＜0.05）．Conclusion Both etomidate and propofol inhibit the cardiovascular stability in elderly patients in different degrees，but etomidate has less effect on the circulation of patients and vascular stability，so etomidate is more suitable as the preferred intravenous anesthetic induction drug for elderly patients
2012, 33(02)
Abstract:
［Abstract］Objective To compare the therapeutic effects on shoulder joint osteoarthritis after shoulder adhesion between itraarticular Hyaluronic Acid Sodium and ozone injection and pain spot block method．Methods The patients were divided intro two groups: study group and control group．Study Group：32 patients were treated by intraarticularly injecting Hyaluronic Acid Sodium 20 ug/mL and ozone 20～40 mL for shoulder joint osteoarthritis after shoulder adhesion．Control Group：30 patients were treated by pain spot block method．Results The treatment efficacy of Study group was significant higher than Control group one week，two week and three weeks after treatment，there was a statistically significant difference between study group and control group（P＜0.05）． Conclusion The treatment by intraarticular Hyaluronic Acid Sodium and ozone injection for shoulder joint osteoarthritis after shoulder adhesion is a safe，effective and short term treatment method
［Abstract］Objective To compare the therapeutic effects on shoulder joint osteoarthritis after shoulder adhesion between itraarticular Hyaluronic Acid Sodium and ozone injection and pain spot block method．Methods The patients were divided intro two groups: study group and control group．Study Group：32 patients were treated by intraarticularly injecting Hyaluronic Acid Sodium 20 ug/mL and ozone 20～40 mL for shoulder joint osteoarthritis after shoulder adhesion．Control Group：30 patients were treated by pain spot block method．Results The treatment efficacy of Study group was significant higher than Control group one week，two week and three weeks after treatment，there was a statistically significant difference between study group and control group（P＜0.05）． Conclusion The treatment by intraarticular Hyaluronic Acid Sodium and ozone injection for shoulder joint osteoarthritis after shoulder adhesion is a safe，effective and short term treatment method
2012, 33(02)
Abstract:
［Abstract］Objective To evaluate the value of Endoscopic Ultrasonography in diagnosis of primary sclerosing cholangitis．Method We performed retrospective analysis on EUS images of 8 cases proven primary sclerosing cholangitis．Results The whole common bile duct wall of 8 cases were thickening and lumens were narrow．Bile duct wall of 3 cases were echo enhancement，intrahepatic bile ducts of 4 cases were mild ectasia，gallbladder volume of 2 cases were augmentation，Portal lymph node of 4 cases were enlargement，Portal Vein of 1 case was slightly ectasia．Conclusion Endoscopic Ultrasonography has good prospects in diagnosis of bile duct diseases
［Abstract］Objective To evaluate the value of Endoscopic Ultrasonography in diagnosis of primary sclerosing cholangitis．Method We performed retrospective analysis on EUS images of 8 cases proven primary sclerosing cholangitis．Results The whole common bile duct wall of 8 cases were thickening and lumens were narrow．Bile duct wall of 3 cases were echo enhancement，intrahepatic bile ducts of 4 cases were mild ectasia，gallbladder volume of 2 cases were augmentation，Portal lymph node of 4 cases were enlargement，Portal Vein of 1 case was slightly ectasia．Conclusion Endoscopic Ultrasonography has good prospects in diagnosis of bile duct diseases
2012, 33(02)
Abstract:
［Abstract］Objectives To explore the difference of liver function in patients with HIV and HCV coinfection before HAART therapy at different CD4 level in Kunming．Methods 325 HIV/HCV coinfected patients before antiretroviral treatment were divided into two groups based CD4 T cell count：CD4＞200 and CD4＜200 group．To test the patients liver function，the serum ALT，AST，TBIL levels of the patients were detected．Comparison of liver function differences was made between two groups．Result There were no significant differences（P＞0.05）in liver function between high and lower CD4 T cell count group．Conclusion There are no significant differeces in liver function between two groups of patients with HIV/HCV coinfection
［Abstract］Objectives To explore the difference of liver function in patients with HIV and HCV coinfection before HAART therapy at different CD4 level in Kunming．Methods 325 HIV/HCV coinfected patients before antiretroviral treatment were divided into two groups based CD4 T cell count：CD4＞200 and CD4＜200 group．To test the patients liver function，the serum ALT，AST，TBIL levels of the patients were detected．Comparison of liver function differences was made between two groups．Result There were no significant differences（P＞0.05）in liver function between high and lower CD4 T cell count group．Conclusion There are no significant differeces in liver function between two groups of patients with HIV/HCV coinfection
2012, 33(02)
Abstract:
［Abstract］Objective To enhance the cognition about clinical characteristics of pulmonary tuberculosis patients with and without HIV(+)/AIDS．Methods The Group A was 42 cases of pulmonary tuberculosis patients with HIV(+)/AIDS，and the GroupB was 50 cases of pulmonary tuberculosis patients without HIV(+)/AIDS，who were admitted in The Third People's Hospital of Kunming from January 2010 to January 2011．A retrospective cohort study was conducted in the two groups．Results There were statistical significant differences between the two groups in the incidence rate of extrapulmonary tuberculosis（χ2＝16.745，P＝0.01），the PPD test（χ2＝7.068，P＝0.008），the examination of immunocompetence（χ2＝77.072，P＝0.01）and the examination of imageology（χ2＝14.313，P＝0.000）．Conclusion The difference between the two groups is obvious in the clinical manifestations， the diagnosis and therapy
［Abstract］Objective To enhance the cognition about clinical characteristics of pulmonary tuberculosis patients with and without HIV(+)/AIDS．Methods The Group A was 42 cases of pulmonary tuberculosis patients with HIV(+)/AIDS，and the GroupB was 50 cases of pulmonary tuberculosis patients without HIV(+)/AIDS，who were admitted in The Third People's Hospital of Kunming from January 2010 to January 2011．A retrospective cohort study was conducted in the two groups．Results There were statistical significant differences between the two groups in the incidence rate of extrapulmonary tuberculosis（χ2＝16.745，P＝0.01），the PPD test（χ2＝7.068，P＝0.008），the examination of immunocompetence（χ2＝77.072，P＝0.01）and the examination of imageology（χ2＝14.313，P＝0.000）．Conclusion The difference between the two groups is obvious in the clinical manifestations， the diagnosis and therapy
2012, 33(02)
Abstract:
［Abstract］For an in-depth discussion on etiology，pathogenesis and drug treatment of diabetes，the establishment of the ideal diabetic animal model is an important way to undertake a study on diabetes mellitus. Preparation method can be divided into spontaneous diabetes mellitus rat model，induced by experimental models，transgenic and gene knockout models，each with its characteristic．This review will be an update to rat models of type 1 and type 2 diabetes mellitus in these years
［Abstract］For an in-depth discussion on etiology，pathogenesis and drug treatment of diabetes，the establishment of the ideal diabetic animal model is an important way to undertake a study on diabetes mellitus. Preparation method can be divided into spontaneous diabetes mellitus rat model，induced by experimental models，transgenic and gene knockout models，each with its characteristic．This review will be an update to rat models of type 1 and type 2 diabetes mellitus in these years
2012, 33(02)
Abstract:
［Abstract］Objective To understand the health status and to analyse the influencing factors of LTC demands among urban community senior citizens．Method Self-designed questionnaire was used to investigate 1 000 senior citizens aged over 60 years in the urban community by multi-stage stratified sampling．Results Senior citizens had various chronic diseases and cardiovascular system disease was the first．In terms of the demand for LTC，elderly day care center and nursing home were predominant．The main influencing factors of LTC demand were the situation of take medicine，depression and the source of medical payment．Conclusions There are high demands of LTC among urban community senior citizens Also，it is necessary to educate general nurses qualified in long-term care for the senior citizens to promote the community development
［Abstract］Objective To understand the health status and to analyse the influencing factors of LTC demands among urban community senior citizens．Method Self-designed questionnaire was used to investigate 1 000 senior citizens aged over 60 years in the urban community by multi-stage stratified sampling．Results Senior citizens had various chronic diseases and cardiovascular system disease was the first．In terms of the demand for LTC，elderly day care center and nursing home were predominant．The main influencing factors of LTC demand were the situation of take medicine，depression and the source of medical payment．Conclusions There are high demands of LTC among urban community senior citizens Also，it is necessary to educate general nurses qualified in long-term care for the senior citizens to promote the community development
2012, 33(02): 1-1.
Abstract:
［Abstract］Objective To investigate the association between the forkhead transcription factor C2（FOXC2）C-1702T transition in the putative promoter region and type 2 diabetes．Methods 280 han people in Yunnan province were selected as subjects. A case-control study was performed in 159 type 2 diabetic patients and 121 control subjects．The FOXC2 gene C-1702T polymorphism was detected by heminested PCR．Results FOXC2 C-1702T allele and genotype distribution did not differ between patients with type 2 diabetes and control subjects（P＞0.05）， and had no association with gender. The C/C genotype was associated with higher level of cholesterol（P＜0.05）among type 2 diabetic patients．Conclusions FOXC2 C-1702T is not associated with gender and type 2 diabetes. FOXC2 C-1702T is associated with serum cholesterol.
［Abstract］Objective To investigate the association between the forkhead transcription factor C2（FOXC2）C-1702T transition in the putative promoter region and type 2 diabetes．Methods 280 han people in Yunnan province were selected as subjects. A case-control study was performed in 159 type 2 diabetic patients and 121 control subjects．The FOXC2 gene C-1702T polymorphism was detected by heminested PCR．Results FOXC2 C-1702T allele and genotype distribution did not differ between patients with type 2 diabetes and control subjects（P＞0.05）， and had no association with gender. The C/C genotype was associated with higher level of cholesterol（P＜0.05）among type 2 diabetic patients．Conclusions FOXC2 C-1702T is not associated with gender and type 2 diabetes. FOXC2 C-1702T is associated with serum cholesterol.
