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RSS2016 Vol. 37, No. 01
2016, 37(01)
Abstract:
Abstract］Hand，foot，and mouth disease is a common infectious disease among children， mainly caused by coxsackievirus type A 16 and enterovirus 71，and has become a serious public health hazard. The biological characteristics and laboratory tests of the two pathogens are reviewed in this paper.
Abstract］Hand，foot，and mouth disease is a common infectious disease among children， mainly caused by coxsackievirus type A 16 and enterovirus 71，and has become a serious public health hazard. The biological characteristics and laboratory tests of the two pathogens are reviewed in this paper.
2016, 37(01)
Abstract:
［Abstract］Objective To study the chemical constituents from the flowers of Dendrobium thyrsiflorum and find its bioactive compounds. Methods The compounds were extracted by 95% alcohol and isolated by column chromatography on silica gel and Sephadex LH-20. Their structures were identified by spectroscopic analysis（1H NMR，13CNMR and EIMS）．Results Seven compouds were obtained and identified as β-sitosterol（1），stigmasterol（2），6，7-dimethoxy coumarin（3），6-methoxy -7- hydroxy coumarin（4），dibutyl phthalate（5），naringenin（6），daucosterol（7）．Conclusion All compounds were isolated from the flowers for the first time.
［Abstract］Objective To study the chemical constituents from the flowers of Dendrobium thyrsiflorum and find its bioactive compounds. Methods The compounds were extracted by 95% alcohol and isolated by column chromatography on silica gel and Sephadex LH-20. Their structures were identified by spectroscopic analysis（1H NMR，13CNMR and EIMS）．Results Seven compouds were obtained and identified as β-sitosterol（1），stigmasterol（2），6，7-dimethoxy coumarin（3），6-methoxy -7- hydroxy coumarin（4），dibutyl phthalate（5），naringenin（6），daucosterol（7）．Conclusion All compounds were isolated from the flowers for the first time.
2016, 37(01)
Abstract:
［Abstract］Objective To study the role of Gnaq，the α subunit of G proteins，in the proliferation of neuroblastoma cell line SH-SY5Y，and further explore the underlying mechanism. Methods Over-expression of Gnaq in SH-SY5Y cells was performed by lentivirus infection. The infection rate was checked by fluorescence microscope. Then MTT experiment was used to evaluate the proliferation of the Gnaq-overexpressed cells. The transcription level of Gnaq and expression level of nuclear factor kappa B （NF-κB）were measured by PCR and Western Blot respectively. Results （1）The infection rate of lentivirus in SH-SY5Y cells was（97.60±2.14）%. （2）The proliferation and NF-κB level of SH-SY5Y cells was significantly upregulated with Gnaq over-expression. Conclusions Gnaq plays an important role in the proliferation of SH-SY5Y cells by regulating the NF-κB signal pathway.
［Abstract］Objective To study the role of Gnaq，the α subunit of G proteins，in the proliferation of neuroblastoma cell line SH-SY5Y，and further explore the underlying mechanism. Methods Over-expression of Gnaq in SH-SY5Y cells was performed by lentivirus infection. The infection rate was checked by fluorescence microscope. Then MTT experiment was used to evaluate the proliferation of the Gnaq-overexpressed cells. The transcription level of Gnaq and expression level of nuclear factor kappa B （NF-κB）were measured by PCR and Western Blot respectively. Results （1）The infection rate of lentivirus in SH-SY5Y cells was（97.60±2.14）%. （2）The proliferation and NF-κB level of SH-SY5Y cells was significantly upregulated with Gnaq over-expression. Conclusions Gnaq plays an important role in the proliferation of SH-SY5Y cells by regulating the NF-κB signal pathway.
2016, 37(01)
Abstract:
［Abstract］Objective To study the expression characteristics of miR-10b and its precursor and Dicer in breast cancer．Method We selected 35 patients with breast cancer and collected their cancer tissues and adjacent normal tissues，then used TRIzol to extract total RNA and real-time quantitative PCR to detect the relative gene expression levels. Results The relative expression levels of miR-10b and Dicer in cancer tissue were significantly lower than in adjacent normal tissue（P＜0.05）. There was a significant correlation between the expression of Dicer and C-erbB-2 （r＝0.60，P＜0.05）and a significant negative correlation between the expression of miR-10b gene and C-erbB-2（rs＝-0.42 ，P＜0.05）. Conclusion The expression levels of miR-10b and Dicer are low in breast cancer tissues， and may contribute to the inhibition of the development of breast cancer.
［Abstract］Objective To study the expression characteristics of miR-10b and its precursor and Dicer in breast cancer．Method We selected 35 patients with breast cancer and collected their cancer tissues and adjacent normal tissues，then used TRIzol to extract total RNA and real-time quantitative PCR to detect the relative gene expression levels. Results The relative expression levels of miR-10b and Dicer in cancer tissue were significantly lower than in adjacent normal tissue（P＜0.05）. There was a significant correlation between the expression of Dicer and C-erbB-2 （r＝0.60，P＜0.05）and a significant negative correlation between the expression of miR-10b gene and C-erbB-2（rs＝-0.42 ，P＜0.05）. Conclusion The expression levels of miR-10b and Dicer are low in breast cancer tissues， and may contribute to the inhibition of the development of breast cancer.
2016, 37(01)
Abstract:
［Abstract］Objective To explore the expressions differences of the FHIT gene in 90 human hepatocellular carcinoma tissues with tissue chip method. Methods 90 cases of patients with primary liver cancer of complete the clinic pathological data and its surgical specimens to keep in the archives with paraffin blocks were collected. FHIT gene expressional differences in the above specimens were determined by IHC-tissue chip. Results Whether qualitative or quantitative analysis，IHC test results showed that there was a significant difference in expressions of FHIT protein between hepatocellular carcinoma tissue and para-carcinoma tissue. The positive expression of FHIT protein in para-carcinoma tissue was significantly higher than hepatocellular carcinoma tissue（P＜0.05）. Conclusions FHIT gene is a cancer suppressor gene and poor expression of FHIT gene is correlated with malignant differentiation of liver cancer. Immunohistochemical method is helpful to assist the early diagnosis and make a reasonable appraisal of their prognosis.
［Abstract］Objective To explore the expressions differences of the FHIT gene in 90 human hepatocellular carcinoma tissues with tissue chip method. Methods 90 cases of patients with primary liver cancer of complete the clinic pathological data and its surgical specimens to keep in the archives with paraffin blocks were collected. FHIT gene expressional differences in the above specimens were determined by IHC-tissue chip. Results Whether qualitative or quantitative analysis，IHC test results showed that there was a significant difference in expressions of FHIT protein between hepatocellular carcinoma tissue and para-carcinoma tissue. The positive expression of FHIT protein in para-carcinoma tissue was significantly higher than hepatocellular carcinoma tissue（P＜0.05）. Conclusions FHIT gene is a cancer suppressor gene and poor expression of FHIT gene is correlated with malignant differentiation of liver cancer. Immunohistochemical method is helpful to assist the early diagnosis and make a reasonable appraisal of their prognosis.
2016, 37(01)
Abstract:
［Abstract］Objective To optimically prepare for biotin-microbubbles with small diameter and good stability，and evaluate the physical and chemical properties，and the effect of contrast．Methods We prepare lipid vesicles with different proportion for the group A-E.The physical and chemical properties of each group were evaluated by particle size analyzer and Malvern．We evaluated the effect of different solvents on the stability of the group1-3，observed the effect of each group by ultrasonic diagnostic apparatus．Results There were significant differences between groups A，Cand E:Lipid located the different layers of the vial；With the increasing of DPPC，MPEG200，DPPA，the average size and concentration decreased gradually，the distribution became uniform gradually，and the Zeta decreased gradually.Complex solution made microbubbles with small size，non-rupture，concentration maintain and good stability．Conclusions DPPC：MPEG200：DPPA：DSPE-PEG2000-Biotin 90:5:5:10 and complex solution can make the best biotin-microbubbles.It is a optimal choice for preparation of target microbubbles.
［Abstract］Objective To optimically prepare for biotin-microbubbles with small diameter and good stability，and evaluate the physical and chemical properties，and the effect of contrast．Methods We prepare lipid vesicles with different proportion for the group A-E.The physical and chemical properties of each group were evaluated by particle size analyzer and Malvern．We evaluated the effect of different solvents on the stability of the group1-3，observed the effect of each group by ultrasonic diagnostic apparatus．Results There were significant differences between groups A，Cand E:Lipid located the different layers of the vial；With the increasing of DPPC，MPEG200，DPPA，the average size and concentration decreased gradually，the distribution became uniform gradually，and the Zeta decreased gradually.Complex solution made microbubbles with small size，non-rupture，concentration maintain and good stability．Conclusions DPPC：MPEG200：DPPA：DSPE-PEG2000-Biotin 90:5:5:10 and complex solution can make the best biotin-microbubbles.It is a optimal choice for preparation of target microbubbles.
2016, 37(01)
Abstract:
［Abstract］Objective To establish the tree shrew osteoporotic model by bilateral ovariectomy．Methods Sixty healthy female tree shrews（Tupaia belangeri chinensis）were divided into operation group（OVX，n＝30） and sham group （SHAM，n＝30）. The bilateral ovaries of the tree shrews in OVX group were operated to resect， while in SHAM omentum majus were removed with the same volume of the ovaries．The BMD on six animals randomly selected in two groups were detected on the day before operation and on 1st，2nd， 3rd，4th，5th and 6th month after operation respectively. Six tree shrews of each group were sacrificed respectively pre-operation and post-operation when there was a difference（P＜0.05）or a significant difference（P＜0.01）in BMD values between SHAM group and OVX group. Then the second lumbar vertebras were sectioned for histomorphometry. Results（1）No death occurred during experiment. （2）The BMD values of tree shrew's whole body in SHAM group at each time point had no difference（P≥0.05）；The trabecula in the second lumbar vertebras was complete， regular and closely arranged. The connection between bone trabecula was normal. Bone marrow cells were rich while fat vacuoles were few. （3）Three months after operation，the BMD value in the OVX group was obviously lower than that in the SHAM's（P<0.05）. The sections of the second lumbar vertebras in OVX group showed that the trabecular bone was broken，thinning and scattered，moreover the trabecular spacing was widened， irregular arranged and the connection between the trabecular meshwork became less while bone marrow cells were reduced and more fat vacuoles appeared. （4）After four months，the whole body BMD in OVX group was significantly lower than that in SHAM'S （P＜0.01）. The sections in OVX group showed the trabecular bone was sparser and thinner than that in the 3rd month， and the trabecular connection was rare while bone marrow cells were further reduced and fat vacuoles became larger.（5）Six months after operation， the whole body BMD value in OVX group declined by 31% with a significant BMD change（P＜0.01）compared with the value in pre-operation. The sections showed thinner trabecula， wider trabecular spacing，less bone marrow cells and more fat vacuoles. Conclusion Bilateral ovariectomy on 6 month-old tree shrews could establish stable osteoporosis model after four months.
［Abstract］Objective To establish the tree shrew osteoporotic model by bilateral ovariectomy．Methods Sixty healthy female tree shrews（Tupaia belangeri chinensis）were divided into operation group（OVX，n＝30） and sham group （SHAM，n＝30）. The bilateral ovaries of the tree shrews in OVX group were operated to resect， while in SHAM omentum majus were removed with the same volume of the ovaries．The BMD on six animals randomly selected in two groups were detected on the day before operation and on 1st，2nd， 3rd，4th，5th and 6th month after operation respectively. Six tree shrews of each group were sacrificed respectively pre-operation and post-operation when there was a difference（P＜0.05）or a significant difference（P＜0.01）in BMD values between SHAM group and OVX group. Then the second lumbar vertebras were sectioned for histomorphometry. Results（1）No death occurred during experiment. （2）The BMD values of tree shrew's whole body in SHAM group at each time point had no difference（P≥0.05）；The trabecula in the second lumbar vertebras was complete， regular and closely arranged. The connection between bone trabecula was normal. Bone marrow cells were rich while fat vacuoles were few. （3）Three months after operation，the BMD value in the OVX group was obviously lower than that in the SHAM's（P<0.05）. The sections of the second lumbar vertebras in OVX group showed that the trabecular bone was broken，thinning and scattered，moreover the trabecular spacing was widened， irregular arranged and the connection between the trabecular meshwork became less while bone marrow cells were reduced and more fat vacuoles appeared. （4）After four months，the whole body BMD in OVX group was significantly lower than that in SHAM'S （P＜0.01）. The sections in OVX group showed the trabecular bone was sparser and thinner than that in the 3rd month， and the trabecular connection was rare while bone marrow cells were further reduced and fat vacuoles became larger.（5）Six months after operation， the whole body BMD value in OVX group declined by 31% with a significant BMD change（P＜0.01）compared with the value in pre-operation. The sections showed thinner trabecula， wider trabecular spacing，less bone marrow cells and more fat vacuoles. Conclusion Bilateral ovariectomy on 6 month-old tree shrews could establish stable osteoporosis model after four months.
2016, 37(01)
Abstract:
［Abstract］Objective To investigate the genetic heterogeneity of thalassemia in the populations of Yunnan province. Methods From July 2009 to Octorber 2012，mutations of the α- or β-hemoglobin genes were tested in suspected thalassemia cases to investigate the spectrums of gene mutations. And the distribution of mutations in different region and minority were analysed．Results Four types of α-thalassemia mutations，--SEA （62.82%），-α3.7（19.66%），αCS（12.39%），and -α4.2 （5.13%）were identified. Seven common mutations were responsible for the β-thalassemia alleles，CD26（32.00%），CD17 （22.18%），CD41-42（18.91%）， IVS-Ⅱ-654 （16.18%），-28（5.09%），CD71-72（2.00%），and CD27-28（0.91%）. For β-thalassemia，CD41-42 was the most frequent mutation in southeast Yunnan and βE was the most frequent mutation in southwest Yunnan．Conclusions The most frequent mutation is βE for β-thalassemia，which is different from other regions in China. Similar to the results obtained in other regions in China previously reported，SEA mutation is the most frequent for α-thalassemia. Our data provide valuable information on the control of thalassemia in the population with ethnic and geographical background in Yunnan Province．
［Abstract］Objective To investigate the genetic heterogeneity of thalassemia in the populations of Yunnan province. Methods From July 2009 to Octorber 2012，mutations of the α- or β-hemoglobin genes were tested in suspected thalassemia cases to investigate the spectrums of gene mutations. And the distribution of mutations in different region and minority were analysed．Results Four types of α-thalassemia mutations，--SEA （62.82%），-α3.7（19.66%），αCS（12.39%），and -α4.2 （5.13%）were identified. Seven common mutations were responsible for the β-thalassemia alleles，CD26（32.00%），CD17 （22.18%），CD41-42（18.91%）， IVS-Ⅱ-654 （16.18%），-28（5.09%），CD71-72（2.00%），and CD27-28（0.91%）. For β-thalassemia，CD41-42 was the most frequent mutation in southeast Yunnan and βE was the most frequent mutation in southwest Yunnan．Conclusions The most frequent mutation is βE for β-thalassemia，which is different from other regions in China. Similar to the results obtained in other regions in China previously reported，SEA mutation is the most frequent for α-thalassemia. Our data provide valuable information on the control of thalassemia in the population with ethnic and geographical background in Yunnan Province．
2016, 37(01)
Abstract:
［Abstract］Objective To investigate expression of vitamin D receptor （VDR） and sodium-dicarboxylate cotransporter protein 1（NaDC1）in human renal proximal tubule epithelial cell （HK-2 cells） and their significance in hypocitraturia. Methods We cultured HK-2 cells under the cultivation condition of HK-2 cells. The apoptosis of HK-2 cells cultured was detected by Flow Cytometry ，and the expression of VDR and NaDC1 in HK-2 cells was detected by immunocytochemical SP method. Results The culture of HK-2 cells: The state of HK-2 cells was recorded by taking pictures. Flow cytometry: HK-2 cells apoptosis rate of flow tube number 1-4 were respectively 6.87%，6.80%，8.31% and 4.274%. Immunocytochemistry : The expression of NaDC1 in HK-2 cells: the cell membrane of HK-2 cells and NaDC1 positive control （human embryonic kidney 293 cells，HEK293 cells） were stained. While cell membrane of NaDC1 negative control was not stained. The expression of VDR in HK-2 cells: the nuclear membrane of HK-2 cells and VDR positive control （human lung cancer cell line A549 cells） were stained. While nuclear membrane of VDR negative control was not stained. Conclusions The apoptosis rate of the HK-2 cells cultured is relatively low，and the cells are in good condition. VDR and NaDC1 are respectively expressed in the nuclear membrane and cell membrane of HK-2 cells. Both VDR and NaDC1 are associated with the occurrence and development of hypocitraturia. We conclude that VDR may be involved in regulating the activity or expression of NaDC1.
［Abstract］Objective To investigate expression of vitamin D receptor （VDR） and sodium-dicarboxylate cotransporter protein 1（NaDC1）in human renal proximal tubule epithelial cell （HK-2 cells） and their significance in hypocitraturia. Methods We cultured HK-2 cells under the cultivation condition of HK-2 cells. The apoptosis of HK-2 cells cultured was detected by Flow Cytometry ，and the expression of VDR and NaDC1 in HK-2 cells was detected by immunocytochemical SP method. Results The culture of HK-2 cells: The state of HK-2 cells was recorded by taking pictures. Flow cytometry: HK-2 cells apoptosis rate of flow tube number 1-4 were respectively 6.87%，6.80%，8.31% and 4.274%. Immunocytochemistry : The expression of NaDC1 in HK-2 cells: the cell membrane of HK-2 cells and NaDC1 positive control （human embryonic kidney 293 cells，HEK293 cells） were stained. While cell membrane of NaDC1 negative control was not stained. The expression of VDR in HK-2 cells: the nuclear membrane of HK-2 cells and VDR positive control （human lung cancer cell line A549 cells） were stained. While nuclear membrane of VDR negative control was not stained. Conclusions The apoptosis rate of the HK-2 cells cultured is relatively low，and the cells are in good condition. VDR and NaDC1 are respectively expressed in the nuclear membrane and cell membrane of HK-2 cells. Both VDR and NaDC1 are associated with the occurrence and development of hypocitraturia. We conclude that VDR may be involved in regulating the activity or expression of NaDC1.
2016, 37(01)
Abstract:
［Abstract］Objective To detect the changs in expression of new fibrosis gene SFTPA2 in rat with renal interstitial fibrosis. Methods Sixty-four SD rats，half male and female,were randomly divided into UUO group，sham-operation group，SiRNA group，negative control group，rats were sacrified 3,5,7,14d after operation （n=4 for each day），and the renal tissues of the operated side（obstructed side for model group）were obtained. HE staining was used to evalute the degree of renal tubulointerstitial fibrosis. ELISA was used to detect the expression of SFTPA2，transforming growth factor-β1 （TGF-β1）α-smooth musdeactin（α-SMA），vascular endothelial grouth factor（VEGF）of blood. Western blot was used to detect the expression of SFTPA2，E-cadherin，α-smooth muscle actin（α-SMA）in renal tissues. Results Histological observations of renal tissue revealed that compared with sham operation group，the degree of renal tubulointerstitial fibrosis gradully increased with time of ureteral obstruction. ELISA showed that compared with sham operation group,the expression of TGF-β1 and SFTPA2 in UUO group increased with time of ureteral obstruction，especially till 7d（P＜0.05），top till 14 d. Compared with negative control group,the expression of TGF-β1 increased till 7d，decreased till 14d（P＜0.05），the expression of SFTPA2 increased till 5d，decreased till 14d（P＜0.05）.Western blot showed that the expression of SFTPA2 increased till 7 d，top till 14d in UUO group；the expression of SFTPA2 increased till 3d，top till 7 d dreased till 14 d，but there was no obvious relationship between them. Conclusion The progression of increase in expression of SFTPA2 in blood and renal tissues of rats may be related to renal interstitial fibrosis. But there is no obvious relationship between SFTPA2 and TGF-β1.
［Abstract］Objective To detect the changs in expression of new fibrosis gene SFTPA2 in rat with renal interstitial fibrosis. Methods Sixty-four SD rats，half male and female,were randomly divided into UUO group，sham-operation group，SiRNA group，negative control group，rats were sacrified 3,5,7,14d after operation （n=4 for each day），and the renal tissues of the operated side（obstructed side for model group）were obtained. HE staining was used to evalute the degree of renal tubulointerstitial fibrosis. ELISA was used to detect the expression of SFTPA2，transforming growth factor-β1 （TGF-β1）α-smooth musdeactin（α-SMA），vascular endothelial grouth factor（VEGF）of blood. Western blot was used to detect the expression of SFTPA2，E-cadherin，α-smooth muscle actin（α-SMA）in renal tissues. Results Histological observations of renal tissue revealed that compared with sham operation group，the degree of renal tubulointerstitial fibrosis gradully increased with time of ureteral obstruction. ELISA showed that compared with sham operation group,the expression of TGF-β1 and SFTPA2 in UUO group increased with time of ureteral obstruction，especially till 7d（P＜0.05），top till 14 d. Compared with negative control group,the expression of TGF-β1 increased till 7d，decreased till 14d（P＜0.05），the expression of SFTPA2 increased till 5d，decreased till 14d（P＜0.05）.Western blot showed that the expression of SFTPA2 increased till 7 d，top till 14d in UUO group；the expression of SFTPA2 increased till 3d，top till 7 d dreased till 14 d，but there was no obvious relationship between them. Conclusion The progression of increase in expression of SFTPA2 in blood and renal tissues of rats may be related to renal interstitial fibrosis. But there is no obvious relationship between SFTPA2 and TGF-β1.
2016, 37(01)
Abstract:
［Abstract］Objective To study the impacts of clostridium，montmorillonite powder and mesalazine on Regulative T Cells （Treg）/ Auxilliary T-17 Cells（ Th17 ）immune balance in rats with ulcerative colitis （UC ）induced by 2，4，6 TNBS/ethanol. Methods 48 rats were divided randomly into 6 groups（8 rats per group） including the model，clostridium，montmorillonite，mesalazine，mesalazine plus montmorillonite and normal group. TNBS/ethanol was used to build a model of rats with UC. The quantity of normal saline， clostridium，montmorillonite powder and mesalazine were converted by surface area of rat and given the rats by gastro-tube daily.4 rats were executed on the 5th day after the molded and treatment， Other rats were executed on the 12th day after the molded and treatment，and the blood samples were collected to measure the contents of Treg and Th17 by a flow cytometer and observe the Treg/Th17 ratios．Results（1）The levels of Treg in normal group and the model group were markedly higher than in clostridium，montmorillonite，mesalazine and mesalazine plus montmorillonite group on the fifth day， differences are statistically.（P＜0.05）However， this result reversed dramaticly on the 12th day， which were more lower， especially in clostridium group.Significant difference appeared except the mesalazine group（P＜0.01）；Statistically comparing the 12th day with the 5th day in the treatment， Treg values all rised tremendously ，excluded the normal group and the model group.（2）The level of Th17 in normal group was obviously more lower than in other 5 groups on the 5th and 12th day， apparently more lower than in the model group.The differences have statistic significance（P＜0.05）；Statistically comparing the 12th day with the 5th day in the treatment，the Th17 value decreased apparently in the other 4 groups excluded the montmorillonite and clostridium group，differences were statistically significant（P＜0.05）．（3）The ratio of Treg/Th17 in normal group was obviously more higher than other 5 groups on the 5th and 12th day， apparently higher than in the model group. （P＜0.05）But on the 12th day， the ratios of Treg/Th17 in clostridium，montmorillonite， mesalazine and mesalazine plus montmorillonite group were gradually close to normal group， especially in mesalazine plus montmorillonite group，（P＜0.05）while the model group still keeps lower level，Statistically comparing the 12th day with the fifth day in the treatment，all the ratio of Treg/Th17 rised（P＜0.05）．Conclusions（1）The immune imbalance of Treg/Th17 might play a important role in UC pathogenesis. （2）Clostridium，montmorillonite，and mesalazine could effectively restrain or reduce the level of Th17 and elevate the level of Treg in rats with UC model to prevent inflammatory reaction and shorten the duration of inflammation.
［Abstract］Objective To study the impacts of clostridium，montmorillonite powder and mesalazine on Regulative T Cells （Treg）/ Auxilliary T-17 Cells（ Th17 ）immune balance in rats with ulcerative colitis （UC ）induced by 2，4，6 TNBS/ethanol. Methods 48 rats were divided randomly into 6 groups（8 rats per group） including the model，clostridium，montmorillonite，mesalazine，mesalazine plus montmorillonite and normal group. TNBS/ethanol was used to build a model of rats with UC. The quantity of normal saline， clostridium，montmorillonite powder and mesalazine were converted by surface area of rat and given the rats by gastro-tube daily.4 rats were executed on the 5th day after the molded and treatment， Other rats were executed on the 12th day after the molded and treatment，and the blood samples were collected to measure the contents of Treg and Th17 by a flow cytometer and observe the Treg/Th17 ratios．Results（1）The levels of Treg in normal group and the model group were markedly higher than in clostridium，montmorillonite，mesalazine and mesalazine plus montmorillonite group on the fifth day， differences are statistically.（P＜0.05）However， this result reversed dramaticly on the 12th day， which were more lower， especially in clostridium group.Significant difference appeared except the mesalazine group（P＜0.01）；Statistically comparing the 12th day with the 5th day in the treatment， Treg values all rised tremendously ，excluded the normal group and the model group.（2）The level of Th17 in normal group was obviously more lower than in other 5 groups on the 5th and 12th day， apparently more lower than in the model group.The differences have statistic significance（P＜0.05）；Statistically comparing the 12th day with the 5th day in the treatment，the Th17 value decreased apparently in the other 4 groups excluded the montmorillonite and clostridium group，differences were statistically significant（P＜0.05）．（3）The ratio of Treg/Th17 in normal group was obviously more higher than other 5 groups on the 5th and 12th day， apparently higher than in the model group. （P＜0.05）But on the 12th day， the ratios of Treg/Th17 in clostridium，montmorillonite， mesalazine and mesalazine plus montmorillonite group were gradually close to normal group， especially in mesalazine plus montmorillonite group，（P＜0.05）while the model group still keeps lower level，Statistically comparing the 12th day with the fifth day in the treatment，all the ratio of Treg/Th17 rised（P＜0.05）．Conclusions（1）The immune imbalance of Treg/Th17 might play a important role in UC pathogenesis. （2）Clostridium，montmorillonite，and mesalazine could effectively restrain or reduce the level of Th17 and elevate the level of Treg in rats with UC model to prevent inflammatory reaction and shorten the duration of inflammation.
2016, 37(01)
Abstract:
［Abstract］Objective To investigate the effect of Slug and Sox9 on the invasion and metastasis of nasopharyngeal carcinoma stem cells. Methods CD133 positive nasopharyngeal carcinoma stem cells were sorted from human nasopharyngeal carcinoma cell line CNE-2．Scratch test was used to detect cell invasion， migration and other biological behaviors when Slug and Sox9 together silenced by siRNA．Results The invasion and migration abilities were significantly higher in CNE - 2 / CD133 + group compared with interference groups and control group at 24 hour 48 hour interference groups had little difference compare with control group，while lower at 48 hour. The metastases ability of CNE - 2/CD133 + group and control group was higher compared with interference groups'. Each interference group had no distinctive difference at each time. Conclusions Silencing of slug and Sox9 has influence on nasopharyngeal cancer progression and metastasis. The relation among low expression tumor recurrence and patient survival time still need further research.
［Abstract］Objective To investigate the effect of Slug and Sox9 on the invasion and metastasis of nasopharyngeal carcinoma stem cells. Methods CD133 positive nasopharyngeal carcinoma stem cells were sorted from human nasopharyngeal carcinoma cell line CNE-2．Scratch test was used to detect cell invasion， migration and other biological behaviors when Slug and Sox9 together silenced by siRNA．Results The invasion and migration abilities were significantly higher in CNE - 2 / CD133 + group compared with interference groups and control group at 24 hour 48 hour interference groups had little difference compare with control group，while lower at 48 hour. The metastases ability of CNE - 2/CD133 + group and control group was higher compared with interference groups'. Each interference group had no distinctive difference at each time. Conclusions Silencing of slug and Sox9 has influence on nasopharyngeal cancer progression and metastasis. The relation among low expression tumor recurrence and patient survival time still need further research.
2016, 37(01)
Abstract:
［Abstract］Objective To investigate the incidence ， gender， season and age distribution of Mycoplasma pneumoniae（MP）infection in children in Kunming. Methods From January 2009 to December 2013，11，473 cases of respiratory infections with suspected Mycoplasma pneumoniae infection in our hospital were given serum test of the antibody of Mycoplasma pneumoniae．Results In recent five years， a total of 11，473 cases of respiratory tract infections in children in our hospital received MP antibody detection，the positive case was 2952 cases，the positive rate was 25.73%. The positive rate in male children and female children was 21.89% and 30.87%， respectively， the difference was statistically significant（ χ2＝118.294，P＜0.001）. The highest antibody positive rate（28.41%）was found in the winter season，followed by in spring（28.24%） and autumn（23.07%）. The lowest positive rate（9.86%）was in 0-1year group，followed by 1-3 years group and 4-6 years group， the highest positive rate（30.43%）was in > 6 years group， the trend chi-square test χ2＝178.208，P＜0.001， showing that the positive rate was growing with the age. The highest positive rate（33.75%）was found in 2011， compare with other years， the differences were statistically significant. Conclusion There was MP infection prevalence in Kunming in 2011. The positive rate in children is growing with the age，the peak of infection is in spring and winter， so the detection and prevention of mycoplasma pneumonia should be strengthened.
［Abstract］Objective To investigate the incidence ， gender， season and age distribution of Mycoplasma pneumoniae（MP）infection in children in Kunming. Methods From January 2009 to December 2013，11，473 cases of respiratory infections with suspected Mycoplasma pneumoniae infection in our hospital were given serum test of the antibody of Mycoplasma pneumoniae．Results In recent five years， a total of 11，473 cases of respiratory tract infections in children in our hospital received MP antibody detection，the positive case was 2952 cases，the positive rate was 25.73%. The positive rate in male children and female children was 21.89% and 30.87%， respectively， the difference was statistically significant（ χ2＝118.294，P＜0.001）. The highest antibody positive rate（28.41%）was found in the winter season，followed by in spring（28.24%） and autumn（23.07%）. The lowest positive rate（9.86%）was in 0-1year group，followed by 1-3 years group and 4-6 years group， the highest positive rate（30.43%）was in > 6 years group， the trend chi-square test χ2＝178.208，P＜0.001， showing that the positive rate was growing with the age. The highest positive rate（33.75%）was found in 2011， compare with other years， the differences were statistically significant. Conclusion There was MP infection prevalence in Kunming in 2011. The positive rate in children is growing with the age，the peak of infection is in spring and winter， so the detection and prevention of mycoplasma pneumonia should be strengthened.
2016, 37(01)
Abstract:
［Abstract］Objective To establish one week endurance exercise rat model，and put DHT sustained-release tablets in rat neck，explore the role of MAPK signal pathway in rat skeletal muscle protein synthesis induced by androgen and exercise．Methods Twenty-four 8 week old male SD rats were randomly divided into four groups: sedentary control group（NC），exercise control group（SP），quiet and androgen group（NCD），exercise plus androgen group（SPD）；NC group and SP group was given sham operation，NCD group and SPD group was given DHT sustained-release tablets in neck；NC group and NCD group were given no special treatment，SP group and SPD group was given 7 days of treadmill training. After modeling，the total gastrocnemius muscle protein of rats were extracted and quantitatively examined， and the protein expression of ERK，p38，JNK was tested by western Blot. Results The total protein expression levels were increased gradually，and there was a significant difference in the gastrocnemius muscle total protein content（P＜0.05） with in NCD and SPD compared with NC group；in SP group， compared with NC group， the level of phosphorylation ERK， p38， and JNK were increased，and in sports and androgen coordination treatment group was the most significant. Conclusions Androgen and exercise may increase the skeletal muscle protein synthesis，and the androgen could strengthen the effect of exercise on protein synthesis. Androgen and exercise may upregulate the expression of phosphorylated ERK，p38 and JNK in skeletal muscle，and SPD group is the most significant; it suggest that MAPK signal pathway may play a role in androgens and exercise.
［Abstract］Objective To establish one week endurance exercise rat model，and put DHT sustained-release tablets in rat neck，explore the role of MAPK signal pathway in rat skeletal muscle protein synthesis induced by androgen and exercise．Methods Twenty-four 8 week old male SD rats were randomly divided into four groups: sedentary control group（NC），exercise control group（SP），quiet and androgen group（NCD），exercise plus androgen group（SPD）；NC group and SP group was given sham operation，NCD group and SPD group was given DHT sustained-release tablets in neck；NC group and NCD group were given no special treatment，SP group and SPD group was given 7 days of treadmill training. After modeling，the total gastrocnemius muscle protein of rats were extracted and quantitatively examined， and the protein expression of ERK，p38，JNK was tested by western Blot. Results The total protein expression levels were increased gradually，and there was a significant difference in the gastrocnemius muscle total protein content（P＜0.05） with in NCD and SPD compared with NC group；in SP group， compared with NC group， the level of phosphorylation ERK， p38， and JNK were increased，and in sports and androgen coordination treatment group was the most significant. Conclusions Androgen and exercise may increase the skeletal muscle protein synthesis，and the androgen could strengthen the effect of exercise on protein synthesis. Androgen and exercise may upregulate the expression of phosphorylated ERK，p38 and JNK in skeletal muscle，and SPD group is the most significant; it suggest that MAPK signal pathway may play a role in androgens and exercise.
2016, 37(01)
Abstract:
［Abstract］Objective To investigate the clinical effect of preoperative neoadjuvant chemotherapy by thalidomide combined with gefitinib for non-small cell lung cancer （NSCLC） patients and provide clinical basis for NSCLC therapy. Methods From 2011/1 to 2013/1，we collected 160 NSCLC cases in our hospital and divided them into 2 groups randomly，80 cases in control group and 80 cases in observation group. The patients in the control group were treated with conventional surgical treatment and patients in the observation group were treated with preoperative neoadjuvant chemotherapy by thalidomide combined with gefitinib before surgery. The clinical effects, the 6 months and 1 year survival rates，and the toxicity effects were observed, and the clinical effects and survival rates between the 2 groups were compared. Results The observation group had a therapeutic efficiency ratio（TER） of 80.0% which was significantly higher than the control group（50.0%），and the difference was statistically significant（P＜0.05）. The 6 months survival rate and 1 year survival rate were 97.5% and 92.5%, which were both significantly higher than those of control group（87.5% and 77.5%），and all differences were statistically significant（P＜0.05）. The toxicity effects of observation group were lower. Conclusions Preoperative neoadjuvant chemotherapy by thalidomide combined with gefitinib for NSCLC therapy is effective and safe，which is worthy of study and further application in clinical treatment..
［Abstract］Objective To investigate the clinical effect of preoperative neoadjuvant chemotherapy by thalidomide combined with gefitinib for non-small cell lung cancer （NSCLC） patients and provide clinical basis for NSCLC therapy. Methods From 2011/1 to 2013/1，we collected 160 NSCLC cases in our hospital and divided them into 2 groups randomly，80 cases in control group and 80 cases in observation group. The patients in the control group were treated with conventional surgical treatment and patients in the observation group were treated with preoperative neoadjuvant chemotherapy by thalidomide combined with gefitinib before surgery. The clinical effects, the 6 months and 1 year survival rates，and the toxicity effects were observed, and the clinical effects and survival rates between the 2 groups were compared. Results The observation group had a therapeutic efficiency ratio（TER） of 80.0% which was significantly higher than the control group（50.0%），and the difference was statistically significant（P＜0.05）. The 6 months survival rate and 1 year survival rate were 97.5% and 92.5%, which were both significantly higher than those of control group（87.5% and 77.5%），and all differences were statistically significant（P＜0.05）. The toxicity effects of observation group were lower. Conclusions Preoperative neoadjuvant chemotherapy by thalidomide combined with gefitinib for NSCLC therapy is effective and safe，which is worthy of study and further application in clinical treatment..
2016, 37(01)
Abstract:
［Abstract］Objective To analyze the osteoporotic fracture disability case，cause the medical staff and patients to pay attention to the dangers of osteoporosis，and to prevent positively the incidence of fractures. Methods The osteoporosis-related fractures patients after discharge were followed up by telephone. The follow-up data included self-care ability and quality of life，and were statistically analyzed，so as to show the effect of fracture on the quality of life of the patients，and this should cause the attention of medical staff and patients. Results （1）In 477 patients，there were 88 cases of spinal fracture and 389 cases of hip fracture. We followed 88 cases of patients with spinal fractures by telephone，six months after fracture，67 cases remained feel back pain，accounting for 76% of the patients with spinal fractures; 48 cases remained feel back pain 12 months after fracture，accounting for 54.5% of the patients with spinal fractures; 29 cases remained feel back pain 18 months after fracture，accounting for 33% of the patients with spinal fractures. （2）Six months after hip fracture，47 cases were able to walk outdoor，accounting for 12.08% of the patients with hip fractures；199 cases were able to walk indoor ，accounting for 51.2% of the patients with hip fractures；143 cases were unable to walk，accounting for 36.76% of the patients with hip fractures; One year after hip fracture，61 cases were able to walk outdoor，accounting for 15.7%；256 cases were able to walk indoor，accounting for 65.8%；72 cases were unable to walk，accounting for 18.5%．（3）Compare outdoor walk half a year and one year, chi-square test，χ2 ＝2.107，P＝0.177，there was no statistically significant difference. Compare indoor walk half a year and one year,chi-square test, χ2 ＝17.199，P＝0.000（＜0.05），the difference was statistically significant. Compare unable to walk half a year and one year, chi-square test，χ2 ＝32.400，P＝0.000（＜0.05），the difference was statistically significant. Conclusions Osteoporotic fractures especially hip fracture resulting in high disability rate，impact on patients quality of life seriously，the time after fracture is an important variable affecting the quality of survival. Medical personnel and patients should pay attention to osteoporosis，prevention of fractures positively.
［Abstract］Objective To analyze the osteoporotic fracture disability case，cause the medical staff and patients to pay attention to the dangers of osteoporosis，and to prevent positively the incidence of fractures. Methods The osteoporosis-related fractures patients after discharge were followed up by telephone. The follow-up data included self-care ability and quality of life，and were statistically analyzed，so as to show the effect of fracture on the quality of life of the patients，and this should cause the attention of medical staff and patients. Results （1）In 477 patients，there were 88 cases of spinal fracture and 389 cases of hip fracture. We followed 88 cases of patients with spinal fractures by telephone，six months after fracture，67 cases remained feel back pain，accounting for 76% of the patients with spinal fractures; 48 cases remained feel back pain 12 months after fracture，accounting for 54.5% of the patients with spinal fractures; 29 cases remained feel back pain 18 months after fracture，accounting for 33% of the patients with spinal fractures. （2）Six months after hip fracture，47 cases were able to walk outdoor，accounting for 12.08% of the patients with hip fractures；199 cases were able to walk indoor ，accounting for 51.2% of the patients with hip fractures；143 cases were unable to walk，accounting for 36.76% of the patients with hip fractures; One year after hip fracture，61 cases were able to walk outdoor，accounting for 15.7%；256 cases were able to walk indoor，accounting for 65.8%；72 cases were unable to walk，accounting for 18.5%．（3）Compare outdoor walk half a year and one year, chi-square test，χ2 ＝2.107，P＝0.177，there was no statistically significant difference. Compare indoor walk half a year and one year,chi-square test, χ2 ＝17.199，P＝0.000（＜0.05），the difference was statistically significant. Compare unable to walk half a year and one year, chi-square test，χ2 ＝32.400，P＝0.000（＜0.05），the difference was statistically significant. Conclusions Osteoporotic fractures especially hip fracture resulting in high disability rate，impact on patients quality of life seriously，the time after fracture is an important variable affecting the quality of survival. Medical personnel and patients should pay attention to osteoporosis，prevention of fractures positively.
2016, 37(01)
Abstract:
［Abstract］Objective To explore the lung cancer related specific miRNA for judging the pathological type and disease progression．Methods The 47 samples were got by multi-stage randomized sampling. TRIzol was used to extract the total of RNA from tissue samples，real-time SYBR Green fluorescent quantitative polymerase chain reaction（PCR）was used for quantitative detection of miRNA-192. The experimental data were statistically analyzed by the SPSS software．Results The expression levels of miRNA-192 in lung cancer tissues of patients severe smoking（over 3 cigrattes one day for more than 2 years ）and drinking were significantly higher than that in patients without smoking and drinking or mild smoking（less than 3 cigrattes one day or less than 2 years）. In the 47 cases， the expression of miRNA-192 had no significant difference in lung cancer tissues （△CT=8.19±2.94） and in normal lung tissues（7.42±2.64）（P＞0.05）. There was no correlation between the expression of miRNA-192 in lung cancer tissues and the clinical data．Conclusions MiRNA-192 is up-regulated in the population with risk factors such as smoking and drinking， and plays an role in the pathogenesis of lung cancer.There was no significant difference in miRNA-192 expression between lung cancer tissues and normal lung tissues，so we can't make sure miRNA-192 as a oncogene or suppressor gene of lung cancer. miRNA-192 can't be used in judging the pathological types，staging and prognosis of lung cancer.
［Abstract］Objective To explore the lung cancer related specific miRNA for judging the pathological type and disease progression．Methods The 47 samples were got by multi-stage randomized sampling. TRIzol was used to extract the total of RNA from tissue samples，real-time SYBR Green fluorescent quantitative polymerase chain reaction（PCR）was used for quantitative detection of miRNA-192. The experimental data were statistically analyzed by the SPSS software．Results The expression levels of miRNA-192 in lung cancer tissues of patients severe smoking（over 3 cigrattes one day for more than 2 years ）and drinking were significantly higher than that in patients without smoking and drinking or mild smoking（less than 3 cigrattes one day or less than 2 years）. In the 47 cases， the expression of miRNA-192 had no significant difference in lung cancer tissues （△CT=8.19±2.94） and in normal lung tissues（7.42±2.64）（P＞0.05）. There was no correlation between the expression of miRNA-192 in lung cancer tissues and the clinical data．Conclusions MiRNA-192 is up-regulated in the population with risk factors such as smoking and drinking， and plays an role in the pathogenesis of lung cancer.There was no significant difference in miRNA-192 expression between lung cancer tissues and normal lung tissues，so we can't make sure miRNA-192 as a oncogene or suppressor gene of lung cancer. miRNA-192 can't be used in judging the pathological types，staging and prognosis of lung cancer.
2016, 37(01)
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［Abstract］Objective To compare the clinical effects of paraffin-embedded cell block and smear in the differential diagnosis of benign and malignant pleural effusion. Methods 80 cases of patients with pleural effusion from May 2012 to May 2015 were randomly selected, each pleural effusion sample was divided into 2 parts, 1 part paraffin-embedded sections were detected by cell block method, and 1 part by cell smear．Results The positive rate of cytology cell block paraffin-embedded sections 28.8%（23/80）was significantly higher than smear 22.5% （18/80），P <0.05, the detection rate of cytopathology diagnosis of cancer 32.5%（26/80）was significantly higher than smear 20.0%（16/80），P <0.05；Among the 80 cases of specimens，31 cases were bloody ascites and pleural effusion，of which 13 cases were malignant，the positive rate was 41.9%；56 cases were non-bloody chest ascites, of which 11 cases were malignant, the positive rate was 19.6%. The positive rate of fluid cytopathology diagnosis of bloody abdominal cavity was significantly higher than non-bloody ascites and pleural effusion，P＜0.05．Conclusion The clinical effect of paraffin-embedded cell block in the differential diagnosis of benign and malignant pleural effusion is better than smear.
［Abstract］Objective To compare the clinical effects of paraffin-embedded cell block and smear in the differential diagnosis of benign and malignant pleural effusion. Methods 80 cases of patients with pleural effusion from May 2012 to May 2015 were randomly selected, each pleural effusion sample was divided into 2 parts, 1 part paraffin-embedded sections were detected by cell block method, and 1 part by cell smear．Results The positive rate of cytology cell block paraffin-embedded sections 28.8%（23/80）was significantly higher than smear 22.5% （18/80），P <0.05, the detection rate of cytopathology diagnosis of cancer 32.5%（26/80）was significantly higher than smear 20.0%（16/80），P <0.05；Among the 80 cases of specimens，31 cases were bloody ascites and pleural effusion，of which 13 cases were malignant，the positive rate was 41.9%；56 cases were non-bloody chest ascites, of which 11 cases were malignant, the positive rate was 19.6%. The positive rate of fluid cytopathology diagnosis of bloody abdominal cavity was significantly higher than non-bloody ascites and pleural effusion，P＜0.05．Conclusion The clinical effect of paraffin-embedded cell block in the differential diagnosis of benign and malignant pleural effusion is better than smear.
2016, 37(01)
Abstract:
［Abstract］Objective To compare the toxicities and recurrence rate with concomitant chemoradiotherapy（CRT） and radiotherapy（RT） alone after radical surgery for high-risk endometrial cancer. Methods A total of 43 patients with high-risk endometrial cancer who received standard surgery treatment from Yunnan Cancer Hospital between Oct. 2011 to Jun. 2014 were selected in this study. CRT was given to 21 patients and RT was given to 22 patients. The toxic reactions and the recurrence rate were compared between two groups. Results In Concurrent chemotherapy and radiotherapy alone group, the incidence of Grade 3 gastrointestinal reaction was 61.90% and 31.82% respectively，the difference was statistically significant（P <0.05）; the incidence of Grade 2 marrow suppression was 66.67% and 18.18% respectively，the difference was significant（P <0.01）；the incidence of Grade 2 neurotoxicity was 33.33% and 13.64%，respectively（P > 0.05）；the overall recurrence rate was 4.76% and 27.27%, respectively （P ＜0.05）. Conclusion Compared with the simple radiotherapy，after the concurrent chemoradiotherapy, the incidence of Grade 3 gastrointestinal reaction may increase，the incidence of Grade 2 Bone marrow suppression may also increase significantly，while the incidence of peripheral neurotoxicity may not increase，the toxicity is acceptable, and the 2-year recurrence rate may decrease.
［Abstract］Objective To compare the toxicities and recurrence rate with concomitant chemoradiotherapy（CRT） and radiotherapy（RT） alone after radical surgery for high-risk endometrial cancer. Methods A total of 43 patients with high-risk endometrial cancer who received standard surgery treatment from Yunnan Cancer Hospital between Oct. 2011 to Jun. 2014 were selected in this study. CRT was given to 21 patients and RT was given to 22 patients. The toxic reactions and the recurrence rate were compared between two groups. Results In Concurrent chemotherapy and radiotherapy alone group, the incidence of Grade 3 gastrointestinal reaction was 61.90% and 31.82% respectively，the difference was statistically significant（P <0.05）; the incidence of Grade 2 marrow suppression was 66.67% and 18.18% respectively，the difference was significant（P <0.01）；the incidence of Grade 2 neurotoxicity was 33.33% and 13.64%，respectively（P > 0.05）；the overall recurrence rate was 4.76% and 27.27%, respectively （P ＜0.05）. Conclusion Compared with the simple radiotherapy，after the concurrent chemoradiotherapy, the incidence of Grade 3 gastrointestinal reaction may increase，the incidence of Grade 2 Bone marrow suppression may also increase significantly，while the incidence of peripheral neurotoxicity may not increase，the toxicity is acceptable, and the 2-year recurrence rate may decrease.
2016, 37(01)
Abstract:
［Abstract］Objective To provide an objective reference to evaluate the efficacy of the RPD restoration. Methods According to the RPD restored history, patients were divided into 2 groups：groupⅠand groupⅡ．Patients in groupⅠhad never restored their missing teeth before．The SAM axioquick recorder was used to record mandibular motion track and rang pre and post RPD treatment. Simultaneously，we recorded the surficial myoelectricity of bilateral masseter and temporalis. At last，we measured one minute masticatory efficiency of each subject by light absorption method．Results（1）The range of chewing movement increased 6 months after RPD treatment，the incisal path showed a good regularity and repeatability in the frontal plane. The width of chewing ring increased from（0.73±0.34）mm to（2.58±1.01）mm（P＜0.05）in group Ⅰ. （2）During border movement，the motion track showed a better smoothness，repetitiveness than previous. The range of opening increased from（31.51±6.37）mm to（61.95±12.42）mm while protrusion increasing from（4.22±2.85）mm to 8.38±1.13mm（P＜0.05） among the subjects with restorative history. （3）All cases' abnormal EMG activity were relieved， while the coordination improving. （4）Two groups' chewing efficiency were improved obviously（P＜<0.05）. Conclusion Clinical application of mandibular trajectory and EMG of masticatory muscle can provide an objective reference to evaluate the efficacy of the RPD restoration.
［Abstract］Objective To provide an objective reference to evaluate the efficacy of the RPD restoration. Methods According to the RPD restored history, patients were divided into 2 groups：groupⅠand groupⅡ．Patients in groupⅠhad never restored their missing teeth before．The SAM axioquick recorder was used to record mandibular motion track and rang pre and post RPD treatment. Simultaneously，we recorded the surficial myoelectricity of bilateral masseter and temporalis. At last，we measured one minute masticatory efficiency of each subject by light absorption method．Results（1）The range of chewing movement increased 6 months after RPD treatment，the incisal path showed a good regularity and repeatability in the frontal plane. The width of chewing ring increased from（0.73±0.34）mm to（2.58±1.01）mm（P＜0.05）in group Ⅰ. （2）During border movement，the motion track showed a better smoothness，repetitiveness than previous. The range of opening increased from（31.51±6.37）mm to（61.95±12.42）mm while protrusion increasing from（4.22±2.85）mm to 8.38±1.13mm（P＜0.05） among the subjects with restorative history. （3）All cases' abnormal EMG activity were relieved， while the coordination improving. （4）Two groups' chewing efficiency were improved obviously（P＜<0.05）. Conclusion Clinical application of mandibular trajectory and EMG of masticatory muscle can provide an objective reference to evaluate the efficacy of the RPD restoration.
2016, 37(01)
Abstract:
［Abstract］Objective To explore and analyze the clinical changes of blood coagulation in patients with gynecologic tumor after tumor resection. Method 128 patients with gynecologic cancer admitted in our hospital from August 2013 to December 2014 were taken as the research objects， these 128 patients with gynecologic tumor were selected as the experimental group，and meanwhile 128 normal women were selected as the control group. The plasma prothrombin time（PT），activated partial thromboplastin time（APTT）， thrombin time（TT），fibrinogen in blood（FIB）and D - dimer level（D-dimer）of the patients with gynecologic tumor before the surgery were monitored and compared with the ones after the surgery and a comparison was made between the patients with gynecologic tumor and the normal women. Results Through the comparison of the plasma prothrombin time（PT），activation activated partial thromboplastin time（APTT）， thrombin time（TT），fibrinogen in blood（FIB） and D - dimer （D - dimer） level of the patients，we found that before the surgery， each indicator of the patients in the experimental group was higher than the control group，after the completion of the surgery，each indicator of the patients in the experimental group was lower than before the surgery. Conclusions The plasma prothrombin time（PT），activated partial thromboplastin time（APTT），thrombin time（TT）， fibrinogen in blood（FIB）and D dimer （D-dimer）levels of the patients with Gynecological tumor patients are in a high level before the surgery，the gynecological patients have a hyperfunction of the blood coagulation，and the patients are in the prethrombotic state，through the monitoring of each indicator of blood coagulation in the patients，the incidence of thrombosis after the completion of the resection performed to the gynecological patients can be reduced .
［Abstract］Objective To explore and analyze the clinical changes of blood coagulation in patients with gynecologic tumor after tumor resection. Method 128 patients with gynecologic cancer admitted in our hospital from August 2013 to December 2014 were taken as the research objects， these 128 patients with gynecologic tumor were selected as the experimental group，and meanwhile 128 normal women were selected as the control group. The plasma prothrombin time（PT），activated partial thromboplastin time（APTT）， thrombin time（TT），fibrinogen in blood（FIB）and D - dimer level（D-dimer）of the patients with gynecologic tumor before the surgery were monitored and compared with the ones after the surgery and a comparison was made between the patients with gynecologic tumor and the normal women. Results Through the comparison of the plasma prothrombin time（PT），activation activated partial thromboplastin time（APTT）， thrombin time（TT），fibrinogen in blood（FIB） and D - dimer （D - dimer） level of the patients，we found that before the surgery， each indicator of the patients in the experimental group was higher than the control group，after the completion of the surgery，each indicator of the patients in the experimental group was lower than before the surgery. Conclusions The plasma prothrombin time（PT），activated partial thromboplastin time（APTT），thrombin time（TT）， fibrinogen in blood（FIB）and D dimer （D-dimer）levels of the patients with Gynecological tumor patients are in a high level before the surgery，the gynecological patients have a hyperfunction of the blood coagulation，and the patients are in the prethrombotic state，through the monitoring of each indicator of blood coagulation in the patients，the incidence of thrombosis after the completion of the resection performed to the gynecological patients can be reduced .
2016, 37(01)
Abstract:
［Abstract］Objective To investigate the clinical efficacy and safety of decreasing blood uric acid by benzene bromine Malone in treatment of hyperuricemia with elderly hypertension and diabetes mellitus. Methods All 100 hyperuricemia in elderly patients with hypertension and diabetes mellitus received the standard drug treatments for blood pressure and blood sugar management. They were divided into study group（uric acid intervention group）and control group. Patients in the study group were given low purine diet and benzbromarone tablet （50 mg/day，course of 1 year）， while patients in the control group were only given low purine die. The indexes of the curative efect and security were the metabolism indexes such as blood uric acid and renal function as well as blood blood sugar and pressure. At the same time we evaluated systemly the side effects of rashes， digestive system， nervous system and urinary system. Results （1）In study group， 3 months， 6 months and 1 years after intervention， the serum uric acid levels were significantly lower than that before intervention and the control group（P＜0.01）. （2）The study group patients' ambulatory blood pressure was significantly lower than that before intervention and the control group after 6， 12months， meanwhile the study group patients' success rate of ambulatory blood pressure level was higher than themselves before the intervention after 6， 12months（P ＜0.05）. （3）The 2 groups of patients' metabolic indexes of related blood sugar and renal function had no difference at each testing point before and after the intervention. （4） Except 2 cases of digestive tract symptoms occurred but tolerated in the 2 groups， there had no other adverse reactions. Conclusion Long-term decreasing blood uric acid with benzene bromine Malone has good efficacy and tolerance.
［Abstract］Objective To investigate the clinical efficacy and safety of decreasing blood uric acid by benzene bromine Malone in treatment of hyperuricemia with elderly hypertension and diabetes mellitus. Methods All 100 hyperuricemia in elderly patients with hypertension and diabetes mellitus received the standard drug treatments for blood pressure and blood sugar management. They were divided into study group（uric acid intervention group）and control group. Patients in the study group were given low purine diet and benzbromarone tablet （50 mg/day，course of 1 year）， while patients in the control group were only given low purine die. The indexes of the curative efect and security were the metabolism indexes such as blood uric acid and renal function as well as blood blood sugar and pressure. At the same time we evaluated systemly the side effects of rashes， digestive system， nervous system and urinary system. Results （1）In study group， 3 months， 6 months and 1 years after intervention， the serum uric acid levels were significantly lower than that before intervention and the control group（P＜0.01）. （2）The study group patients' ambulatory blood pressure was significantly lower than that before intervention and the control group after 6， 12months， meanwhile the study group patients' success rate of ambulatory blood pressure level was higher than themselves before the intervention after 6， 12months（P ＜0.05）. （3）The 2 groups of patients' metabolic indexes of related blood sugar and renal function had no difference at each testing point before and after the intervention. （4） Except 2 cases of digestive tract symptoms occurred but tolerated in the 2 groups， there had no other adverse reactions. Conclusion Long-term decreasing blood uric acid with benzene bromine Malone has good efficacy and tolerance.
2016, 37(01)
Abstract:
［Abstract］Objective To explore the feasibility of the body surfaces positioned approach （BSPA）of the right internal jugular vein indwelling caffed catheter（RIVICC）in hemodialysis. Methods The patients treated with the RIVICC in the Blood Purification Center of Yanan Hospital affiliated to Kunming Medical University，from February，2013 to March 2014 were selected. The measurement indexes of vascular access included: instant vascular access after the catheter setup，catheter adjustment during or after the operation，blood flow after adjustment，and Catheter tip in the place in X-ray picture. Results There were a total of 50 patients were received the RIVICDC， 25 using BSPA and 25 using DSA. Of the 25 with BSPA，12% patients experienced an instant vascular access problem after the catheter setup and a Catheter adjustment，92% ones experienced the Catheter tip in the right place in X-ray picture. Of the 25 with DSA，16% patients experienced an instant vascular access problem after the catheter setup and a Catheter adjustment，100% ones experienced the Catheter tip in the right place in X-ray picture. There was no statistical significant differences for all statistics of vascular access between the 2 groups（P＞0.05）．Conclusion BSPA of the RIVICC is safe and feasible.
［Abstract］Objective To explore the feasibility of the body surfaces positioned approach （BSPA）of the right internal jugular vein indwelling caffed catheter（RIVICC）in hemodialysis. Methods The patients treated with the RIVICC in the Blood Purification Center of Yanan Hospital affiliated to Kunming Medical University，from February，2013 to March 2014 were selected. The measurement indexes of vascular access included: instant vascular access after the catheter setup，catheter adjustment during or after the operation，blood flow after adjustment，and Catheter tip in the place in X-ray picture. Results There were a total of 50 patients were received the RIVICDC， 25 using BSPA and 25 using DSA. Of the 25 with BSPA，12% patients experienced an instant vascular access problem after the catheter setup and a Catheter adjustment，92% ones experienced the Catheter tip in the right place in X-ray picture. Of the 25 with DSA，16% patients experienced an instant vascular access problem after the catheter setup and a Catheter adjustment，100% ones experienced the Catheter tip in the right place in X-ray picture. There was no statistical significant differences for all statistics of vascular access between the 2 groups（P＞0.05）．Conclusion BSPA of the RIVICC is safe and feasible.
2016, 37(01)
Abstract:
［Abstract］Objective To quantitatively analyze the iron deposition in deep brain gray nucleus and frontal white matter in healthy adults and its correlation with age using susceptibility-weighted imaging（SWI） with 3.0 Tesla scanner. Methods 157 healthy subjects（age 20 to 85）were scanned with routine sequences and SWI sequences. The subjects were divided into 6 groups according to their age: Group A （age 20 -29），Group B （age 30 -31），Group C（age 40 - 49），Group D （age 50 - 59），Group E（age 60 - 69）and Group F （age > 70）. Phase value was measured in both sides of frontal white matter，red nucleus，substantia nigra pars reticulate，substantia nigra pars compacta，putamen，globus pallidus，head of caudate nucleus and thalamus， and the correlations between the phase value and age was analyzed．Results The phase value of red nucleus was the lowest，followed by substantia nigra reticulate. The highest phase value was found in the frontal white matter. The difference of phase value among some age groups had statistical significance in head of caudate nucleus（P＜0.05）．Obvious correlations between age and phase value was shown in head of caudate nucleus（r＝0.352，P＜0.01）. Conclusion SWI can clearly display the cerebral nuclei， evaluate the brain iron content accurately and age-related iron concentration changes．
［Abstract］Objective To quantitatively analyze the iron deposition in deep brain gray nucleus and frontal white matter in healthy adults and its correlation with age using susceptibility-weighted imaging（SWI） with 3.0 Tesla scanner. Methods 157 healthy subjects（age 20 to 85）were scanned with routine sequences and SWI sequences. The subjects were divided into 6 groups according to their age: Group A （age 20 -29），Group B （age 30 -31），Group C（age 40 - 49），Group D （age 50 - 59），Group E（age 60 - 69）and Group F （age > 70）. Phase value was measured in both sides of frontal white matter，red nucleus，substantia nigra pars reticulate，substantia nigra pars compacta，putamen，globus pallidus，head of caudate nucleus and thalamus， and the correlations between the phase value and age was analyzed．Results The phase value of red nucleus was the lowest，followed by substantia nigra reticulate. The highest phase value was found in the frontal white matter. The difference of phase value among some age groups had statistical significance in head of caudate nucleus（P＜0.05）．Obvious correlations between age and phase value was shown in head of caudate nucleus（r＝0.352，P＜0.01）. Conclusion SWI can clearly display the cerebral nuclei， evaluate the brain iron content accurately and age-related iron concentration changes．
2016, 37(01)
Abstract:
［Abstract］Objective To investigate the value of detecting the paratracheal lymph nodes by ultrasound in diagnosis of autoimmune thyroiditis （AIT）. Methods We selected 101 AIT cases as experimental group. At the same period，35 cases of Graves'disease（GD），31 cases of subacute thyroiditis（SAT），and 60 normal cases were selected as control groups. Lab tests，such as TSH，TGAb，and TPOAb were carried out and the morphology of thyroid，characteristics of two dimensional ultrasound， and colorful Doppler flow were observed. Meanwhile， whether displayed the paratracheal lymph node or not，the aspect ratio of the displayed paratracheal lymph node， corticomedullary differentiation，and the characteristics of colorful blood flow were observed. Results There were significant difference in lab test indicators （TSH， TPOAB，TGAB） among AIT group，GD group，and SAT group（P＜0.05）. There were significant differences in displayed rate of paratracheal lymph node， number of paratracheal lymph node， aspect ratio of the displayed paratracheal lymph node and colorful Doppler flow among AIT group， GD group， SAT group， and normal group（P＜0.05）， but the indicator of corticomedullary differentiation had no significant difference（P＞0.05）. Conclusion Ultrasound examination of the paratracheal lymph node has the diagnostic value in diagnosis of autoimmune thyroiditis，and may assist us to judge the nature of diffused thyroid diseases.
［Abstract］Objective To investigate the value of detecting the paratracheal lymph nodes by ultrasound in diagnosis of autoimmune thyroiditis （AIT）. Methods We selected 101 AIT cases as experimental group. At the same period，35 cases of Graves'disease（GD），31 cases of subacute thyroiditis（SAT），and 60 normal cases were selected as control groups. Lab tests，such as TSH，TGAb，and TPOAb were carried out and the morphology of thyroid，characteristics of two dimensional ultrasound， and colorful Doppler flow were observed. Meanwhile， whether displayed the paratracheal lymph node or not，the aspect ratio of the displayed paratracheal lymph node， corticomedullary differentiation，and the characteristics of colorful blood flow were observed. Results There were significant difference in lab test indicators （TSH， TPOAB，TGAB） among AIT group，GD group，and SAT group（P＜0.05）. There were significant differences in displayed rate of paratracheal lymph node， number of paratracheal lymph node， aspect ratio of the displayed paratracheal lymph node and colorful Doppler flow among AIT group， GD group， SAT group， and normal group（P＜0.05）， but the indicator of corticomedullary differentiation had no significant difference（P＞0.05）. Conclusion Ultrasound examination of the paratracheal lymph node has the diagnostic value in diagnosis of autoimmune thyroiditis，and may assist us to judge the nature of diffused thyroid diseases.
2016, 37(01)
Abstract:
［Abstract］Objective To evaluate the short-time prognostic value of combination of Heart-type fatty acid-binding protein (H-FABP) and simple Pulmonary Embolism Severity Index (sPESI) in normotensive patients with acute pulmonary embolism (APE). Methods One hundred and thirty normotensive patients with APE were enrolled. The plasma concentration of H-FABP was analyzed and the sPESI scores were calculated．Result 13(10%) patients had adverse 30-day outcome. The H-FABP［OR, 4.96（1.70-14.42），P = 0.003］and sPESI ［OR, 9.50（2.24-40.28），P = 0.002］were predictors for short-term prognosis of normotensive APE. All of the 30 patients (23.1%) with sPESI score 0 and H-FABP＜6 μg/L had no adverse 30-day outcome. The combination model of H-FABP ≥6 μg/L with sPESI≥1 point(s) was associated with increased prognostic sensitivity and negative predictive value, which both reached 100%．Conclusions The H-FABP assay and the sPESI are useful prognostic indicators for 30-day outcome of normotensive APE. The combination of the both predictors can yield additive prognostic information and particularly identify low-risk patients with APE.
［Abstract］Objective To evaluate the short-time prognostic value of combination of Heart-type fatty acid-binding protein (H-FABP) and simple Pulmonary Embolism Severity Index (sPESI) in normotensive patients with acute pulmonary embolism (APE). Methods One hundred and thirty normotensive patients with APE were enrolled. The plasma concentration of H-FABP was analyzed and the sPESI scores were calculated．Result 13(10%) patients had adverse 30-day outcome. The H-FABP［OR, 4.96（1.70-14.42），P = 0.003］and sPESI ［OR, 9.50（2.24-40.28），P = 0.002］were predictors for short-term prognosis of normotensive APE. All of the 30 patients (23.1%) with sPESI score 0 and H-FABP＜6 μg/L had no adverse 30-day outcome. The combination model of H-FABP ≥6 μg/L with sPESI≥1 point(s) was associated with increased prognostic sensitivity and negative predictive value, which both reached 100%．Conclusions The H-FABP assay and the sPESI are useful prognostic indicators for 30-day outcome of normotensive APE. The combination of the both predictors can yield additive prognostic information and particularly identify low-risk patients with APE.
2016, 37(01)
Abstract:
［Abstract］Objective To explore the influential factors of pulmonary function in patients with chronic obstructive pulmonary disease（COPD）in Kunming district. Methods A total of 300 patients with COPD were selected from 5 tertiary hospitals in Kunming district as subjects investigated. The subjects were investigated by self-designed questionnaire，and then the influential factors of patients with COPD were analyzed. Results By linear correlation analysis, there were positive correlation between each of degree of education, economic income , the number of quitting smoking and percentage of the forced expiratory volume in one second divided by the predicted value（FEV1%pre）（r values respectively were 0.318，0.309，0.113，P values respectively were 0.000，0.000，0.05），there were negative correlation between smoking index，tobacco dependence score and FEV1%pre（r values respectively were -0.202，-0.323， P values respectively were 0.000，0.000）. By multiple linear regression analysis，FEV1%pre=34.792-1.765×tobacco dependence score+2.558×degree of education+2.587×economic income， in the nine influential factors（gender，age，body mass index， degree of education，economic income，smoking index，whether to give up smoking，number of quitting smoking， tobacco dependence score），the tobacco dependence score was the main factor affecting FEV1%pre. Conclusions The higher the degree of education，economic income of the patients with COPD have，the longer number of years of the patients with COPD quit smoking，the lower smoking index and tobacco dependence score tobacco dependence score have，the higher the FEV1%pre of the patients with COPD have. The tobacco dependence score is the main factor affecting FEV1%pre.
［Abstract］Objective To explore the influential factors of pulmonary function in patients with chronic obstructive pulmonary disease（COPD）in Kunming district. Methods A total of 300 patients with COPD were selected from 5 tertiary hospitals in Kunming district as subjects investigated. The subjects were investigated by self-designed questionnaire，and then the influential factors of patients with COPD were analyzed. Results By linear correlation analysis, there were positive correlation between each of degree of education, economic income , the number of quitting smoking and percentage of the forced expiratory volume in one second divided by the predicted value（FEV1%pre）（r values respectively were 0.318，0.309，0.113，P values respectively were 0.000，0.000，0.05），there were negative correlation between smoking index，tobacco dependence score and FEV1%pre（r values respectively were -0.202，-0.323， P values respectively were 0.000，0.000）. By multiple linear regression analysis，FEV1%pre=34.792-1.765×tobacco dependence score+2.558×degree of education+2.587×economic income， in the nine influential factors（gender，age，body mass index， degree of education，economic income，smoking index，whether to give up smoking，number of quitting smoking， tobacco dependence score），the tobacco dependence score was the main factor affecting FEV1%pre. Conclusions The higher the degree of education，economic income of the patients with COPD have，the longer number of years of the patients with COPD quit smoking，the lower smoking index and tobacco dependence score tobacco dependence score have，the higher the FEV1%pre of the patients with COPD have. The tobacco dependence score is the main factor affecting FEV1%pre.
2016, 37(01)
Abstract:
［Abstract］Objective To study clinical efficacy of tinidazole alone and tinidazole combined with live- lactobacillus-bacteria capsule for bacterial vaginal disease according to the different methods．Methods 300 cases of bacterial vaginal disease were divided into three groups，group A:tinidazole was used alone into the vagina deep， one tablet each time，once every night for 7 days for a period of treatment；group B : tinidazole was used alone one tablet each time，once every night for 7 days ，after that live-lactobacillus bacteria capsule was used every night， one tablet each time for 10 days，continuous17 days for a period of treatment；Group C：tinidazole and live-lactobacillus bacteria capsule was used alternately on the morning and evening of the same day，10 days for a period of treatment．Then analysis of three kinds of clinical efficacy of treatment and relapse rate．Results We analyzed the laboratory and clinical indicators of three groups of cases after treatment 1 week，1 month，3 months ，and found that long curative effect in group B and C group was superior to group A. Three groups of treatment after 3 months，the difference between group A and group B was statistically significant（P < 0.05）；the difference between group C and group A was statistically significant （P < 0.05）；Group B and group C had no statistical significance（P > 0.05）．Conclusion The antibacterial drug combined with probiotics that make lactobacillus predominates at the same time， can restore vaginal flora balance， fundamentally prevent bacterial vaginal disease recurrence， is the key in treatment of the disease.
［Abstract］Objective To study clinical efficacy of tinidazole alone and tinidazole combined with live- lactobacillus-bacteria capsule for bacterial vaginal disease according to the different methods．Methods 300 cases of bacterial vaginal disease were divided into three groups，group A:tinidazole was used alone into the vagina deep， one tablet each time，once every night for 7 days for a period of treatment；group B : tinidazole was used alone one tablet each time，once every night for 7 days ，after that live-lactobacillus bacteria capsule was used every night， one tablet each time for 10 days，continuous17 days for a period of treatment；Group C：tinidazole and live-lactobacillus bacteria capsule was used alternately on the morning and evening of the same day，10 days for a period of treatment．Then analysis of three kinds of clinical efficacy of treatment and relapse rate．Results We analyzed the laboratory and clinical indicators of three groups of cases after treatment 1 week，1 month，3 months ，and found that long curative effect in group B and C group was superior to group A. Three groups of treatment after 3 months，the difference between group A and group B was statistically significant（P < 0.05）；the difference between group C and group A was statistically significant （P < 0.05）；Group B and group C had no statistical significance（P > 0.05）．Conclusion The antibacterial drug combined with probiotics that make lactobacillus predominates at the same time， can restore vaginal flora balance， fundamentally prevent bacterial vaginal disease recurrence， is the key in treatment of the disease.
2016, 37(01)
Abstract:
［Abstract］Objective To analyze the expression of Glioma CD133 and ABCG2 with the treatment of X-ray radiation combined with chemotherapy，and to provide a reference for clinical treatment. Methods Sixty-four patients with gliomas in hospital from January 2013 to February 2015 were selected， and divided into High-level group（39 cases）and the low-level group（25 cases）according to the grade of gliomas. Patients in two groups were given treatment of X-ray radiation combined and chemotherapy，the expression changes of Glioma stem cell marker ADCGG and CD133 before and after intervention were ompared between the two groups. Results CD133 expression （89.7%）and ABCG2 expression（92.3%）of High-level group were significantly lower than CD133 expression group（68.0%）and ABCG2 expression（81.8%） of low-level group before intervention（P＜0.05）. High-level group and the low level of CD133 and ABCG2 expression group were significantly decreased（P＜0.05）. CD133 expression（56.4%）and ABCG2 expression（56.4%）of High-level group were significantly lower than CD133 expression group（20.0%） and ABCG2 expression （24.0%） of low-level group after intervention（P＜0.05）. After the intervention，CD133（r = -0.782） and ABCG2 （r = -0.652） expression and cell deterioration degree had negative correlation（P＜0.05）．Conclusion There are some differences in the expression of CD133 and ABCG2 in glioma cells， and the expression of CD133 and ABCG2 could be improved by the combination of X-ray and radiation.
［Abstract］Objective To analyze the expression of Glioma CD133 and ABCG2 with the treatment of X-ray radiation combined with chemotherapy，and to provide a reference for clinical treatment. Methods Sixty-four patients with gliomas in hospital from January 2013 to February 2015 were selected， and divided into High-level group（39 cases）and the low-level group（25 cases）according to the grade of gliomas. Patients in two groups were given treatment of X-ray radiation combined and chemotherapy，the expression changes of Glioma stem cell marker ADCGG and CD133 before and after intervention were ompared between the two groups. Results CD133 expression （89.7%）and ABCG2 expression（92.3%）of High-level group were significantly lower than CD133 expression group（68.0%）and ABCG2 expression（81.8%） of low-level group before intervention（P＜0.05）. High-level group and the low level of CD133 and ABCG2 expression group were significantly decreased（P＜0.05）. CD133 expression（56.4%）and ABCG2 expression（56.4%）of High-level group were significantly lower than CD133 expression group（20.0%） and ABCG2 expression （24.0%） of low-level group after intervention（P＜0.05）. After the intervention，CD133（r = -0.782） and ABCG2 （r = -0.652） expression and cell deterioration degree had negative correlation（P＜0.05）．Conclusion There are some differences in the expression of CD133 and ABCG2 in glioma cells， and the expression of CD133 and ABCG2 could be improved by the combination of X-ray and radiation.
2016, 37(01)
Abstract:
［Abstract］Objective To investigate and compare the effects of sevoflurane and propofol on one lung ventilation （OLV） induced ischemia-reperfusion injury. Methods Thirty-six patients undergoing thoracic surgery with OLV were randomized in two groups: sevoflurane group（Group S，n = 18 ）and propofol group（ Group P，n = 18）. Anesthesia was inducted with thiopental and was maintained with 1-2.5% of sevoflurane within the 40/60% of O2/N2O mixture in Group S. In Group P，anesthesia was inducted with propofol and was maintained with infusion of propofol and remifentanil. Hemodynamic parameters were monitored and blood samples were obtained before anesthesia induction（T 1）， 1 min before two lung ventilation（T 2），30 min after two lung ventilation（T 3）， and postoperative sixth hours（T 4）. Blood samples for the blood gas，ischemia-modified albumin（IMA），and malonyldialdehyde（MDA）were mensurated .Results Heart rate at T 2 and T3 in Group P was lower than that in Group S，but there was no difference（P＞0.05）.While there was no significant difference in terms of pCO2 and pH（P ＞0.05）.pO2 at T2 and T3 in Group S was significantly lower than that in Group P（P <0.05）. IMA levels and MDA contents at T 4 in Group S were significantly lower than those in Group P（P <0.05）. Conclusion Sevoflurane may offer protection against ischemia-reperfusion injury after OLV in thoracic surgery.
［Abstract］Objective To investigate and compare the effects of sevoflurane and propofol on one lung ventilation （OLV） induced ischemia-reperfusion injury. Methods Thirty-six patients undergoing thoracic surgery with OLV were randomized in two groups: sevoflurane group（Group S，n = 18 ）and propofol group（ Group P，n = 18）. Anesthesia was inducted with thiopental and was maintained with 1-2.5% of sevoflurane within the 40/60% of O2/N2O mixture in Group S. In Group P，anesthesia was inducted with propofol and was maintained with infusion of propofol and remifentanil. Hemodynamic parameters were monitored and blood samples were obtained before anesthesia induction（T 1）， 1 min before two lung ventilation（T 2），30 min after two lung ventilation（T 3）， and postoperative sixth hours（T 4）. Blood samples for the blood gas，ischemia-modified albumin（IMA），and malonyldialdehyde（MDA）were mensurated .Results Heart rate at T 2 and T3 in Group P was lower than that in Group S，but there was no difference（P＞0.05）.While there was no significant difference in terms of pCO2 and pH（P ＞0.05）.pO2 at T2 and T3 in Group S was significantly lower than that in Group P（P <0.05）. IMA levels and MDA contents at T 4 in Group S were significantly lower than those in Group P（P <0.05）. Conclusion Sevoflurane may offer protection against ischemia-reperfusion injury after OLV in thoracic surgery.
2016, 37(01)
Abstract:
［Abstract］Objective To explore the value of large dose of ambroxol hydrochloride in treating patients with acute exacerbation of chronic obstructive pulmonary disease（AECOPD）. Methods Sixty hospitalized patients with moderate-to-severe AECOPD were dived into treatment group and control group at random. The control group was given routine therapy which contained anti-inflammatory，oxygen therapy， antiasthmatic therapy and so on. The treatment group was given ambroxol hydrochloride intravenous drip therapy on basis of the routine therapy. The malonaldehyde（MDA），glutathione reductase（GSH），superoxide dismutase（SOD），FEV1%pre， dyspnea score and so on were measured before and ten days，twenty days after treatment in two groups. The outcomes of two groups were compared. Results In the treatment group，the content of MDA of treatment after treatment ten days and twenty days was lower， the content of GSH and SOD were higher than before treatment；the content of MDA of after treatment twenty days was decreased，the content of GSH and SOD were higher than after treatment ten days， the difference had statistical significance（P＜0.05）. In the treatment group， the FEV1 % pre of after treatment ten days and twenty days were higher than before treatment，the FEV1 % pre of after treatment twenty days were higher than after treatment ten days， the difference had statistical significance（P＜0.05）. In the treatment group，the dyspnea score of after treatment ten days and twenty days was decreased than before treatment，the dyspnea score of after treatment twenty days was decreased than after treatment ten days，the difference had statistical significance（P＜0.05）.The difference of MDA，GSH，SOD，FEV1%pre between after treatment ten days and before treatment, between after treatment twenty days and before treatment, between after treatment ten days and twenty days, and the difference of dyspnea score between after treatment ten days and twenty days in the treatment group and control group all had statistical significance（P＜0.05） .Hospitalized days of the treatment group was（11.13±1.94 ）days，that of the control group was（13.50± 2.06）days， the average hospitalized days of the treatment group was shorter than the control group，the difference was statistically significance（P＜0.05）. In treatment group，only two patients had mild gastrointestinal reaction which was relieved after treatment. Conclusion Large dose of ambroxol hydrochloride can increase antioxidative ability， reduce oxidative stress degree of patients with AECOPD， meanwhile can improve patients′ pulmonary function and shorten hospitalized days.
［Abstract］Objective To explore the value of large dose of ambroxol hydrochloride in treating patients with acute exacerbation of chronic obstructive pulmonary disease（AECOPD）. Methods Sixty hospitalized patients with moderate-to-severe AECOPD were dived into treatment group and control group at random. The control group was given routine therapy which contained anti-inflammatory，oxygen therapy， antiasthmatic therapy and so on. The treatment group was given ambroxol hydrochloride intravenous drip therapy on basis of the routine therapy. The malonaldehyde（MDA），glutathione reductase（GSH），superoxide dismutase（SOD），FEV1%pre， dyspnea score and so on were measured before and ten days，twenty days after treatment in two groups. The outcomes of two groups were compared. Results In the treatment group，the content of MDA of treatment after treatment ten days and twenty days was lower， the content of GSH and SOD were higher than before treatment；the content of MDA of after treatment twenty days was decreased，the content of GSH and SOD were higher than after treatment ten days， the difference had statistical significance（P＜0.05）. In the treatment group， the FEV1 % pre of after treatment ten days and twenty days were higher than before treatment，the FEV1 % pre of after treatment twenty days were higher than after treatment ten days， the difference had statistical significance（P＜0.05）. In the treatment group，the dyspnea score of after treatment ten days and twenty days was decreased than before treatment，the dyspnea score of after treatment twenty days was decreased than after treatment ten days，the difference had statistical significance（P＜0.05）.The difference of MDA，GSH，SOD，FEV1%pre between after treatment ten days and before treatment, between after treatment twenty days and before treatment, between after treatment ten days and twenty days, and the difference of dyspnea score between after treatment ten days and twenty days in the treatment group and control group all had statistical significance（P＜0.05） .Hospitalized days of the treatment group was（11.13±1.94 ）days，that of the control group was（13.50± 2.06）days， the average hospitalized days of the treatment group was shorter than the control group，the difference was statistically significance（P＜0.05）. In treatment group，only two patients had mild gastrointestinal reaction which was relieved after treatment. Conclusion Large dose of ambroxol hydrochloride can increase antioxidative ability， reduce oxidative stress degree of patients with AECOPD， meanwhile can improve patients′ pulmonary function and shorten hospitalized days.
2016, 37(01)
Abstract:
［Abstract］Objective To study the anesthesia effects and application value of remifentanil anesthesia in percutaneous nephroscope lithotripsy. Methods We selected 60 undergoing elective PCNL hospitalized patients during July 2012 - August 2014 of the hospital. Then divided the patients randomly into two groups，30 cases in each group，control group and observation group，both of the groups were applied general anesthesia in preparation and midazolam，propofol，vecuronium bromide and fentanyl injection anesthetic induction to apply endotracheal intubation anesthesia. During the Surgery，we applied fentanyl & propofol on the control group to maintain anesthesia and given remifentanil & propofol to the observation group. Then we observed the narcotic effect of two groups. Results The operation time，postoperative awake time， the dosage of anesthetic drugs， blood loss between the two groups of patients had no significant difference（P > 0.05）；cmpared with the observation group（P < 0.05），control group's T3 points and T0 points increased. And the same time， two groups had no adverse reactions occurred during anesthesia. Conclusion The effect of general anesthesia using remifentanil in percutaneous nephroscope lithotripsy is positive， and is worthy of promotion.
［Abstract］Objective To study the anesthesia effects and application value of remifentanil anesthesia in percutaneous nephroscope lithotripsy. Methods We selected 60 undergoing elective PCNL hospitalized patients during July 2012 - August 2014 of the hospital. Then divided the patients randomly into two groups，30 cases in each group，control group and observation group，both of the groups were applied general anesthesia in preparation and midazolam，propofol，vecuronium bromide and fentanyl injection anesthetic induction to apply endotracheal intubation anesthesia. During the Surgery，we applied fentanyl & propofol on the control group to maintain anesthesia and given remifentanil & propofol to the observation group. Then we observed the narcotic effect of two groups. Results The operation time，postoperative awake time， the dosage of anesthetic drugs， blood loss between the two groups of patients had no significant difference（P > 0.05）；cmpared with the observation group（P < 0.05），control group's T3 points and T0 points increased. And the same time， two groups had no adverse reactions occurred during anesthesia. Conclusion The effect of general anesthesia using remifentanil in percutaneous nephroscope lithotripsy is positive， and is worthy of promotion.
2016, 37(01)
Abstract:
［Abstract］Diabetes is one of noncommunicable diseases which is the main current threat to human health. Study founds that chemokines are not only involved in the incidence of diabetes but also related to the development of diabetic complications closely. C - C subtribe chemokine has become a hot of researche in the pathogenesis of inflammatory diseases. RANTES is a type of cytokines which is the member of cysteine-cysteune chemokines. We will review the relationships between RANTES and CCR5 with diabetes.
［Abstract］Diabetes is one of noncommunicable diseases which is the main current threat to human health. Study founds that chemokines are not only involved in the incidence of diabetes but also related to the development of diabetic complications closely. C - C subtribe chemokine has become a hot of researche in the pathogenesis of inflammatory diseases. RANTES is a type of cytokines which is the member of cysteine-cysteune chemokines. We will review the relationships between RANTES and CCR5 with diabetes.
2016, 37(01)
Abstract:
［Abstract］Objective To understand the status of autonomous learning and find out the influencing factors of our university students，to provide some scientific basis to develop the autonomous learning theory. Methods We collected the data by stratified cluster sampling between 2010 and 2011 grade of certain Medical University students in 5 majors. And the binary Logistic regression was used to analyze the influencing factors. Results（1）79.5% in 519 students has carried out autonomous learning，but 70.42% of students thought autonomous learning not a good learning method. （2）Just 44.3% students indicated that they had got the effective guidance about autonomous learning from teacher. （3）Students who with learning plan were more likely（1.981 times）to carry out autonomous learning than who without learning plan. Conclusion Making a reasonable study plan can guide students to study independently.
［Abstract］Objective To understand the status of autonomous learning and find out the influencing factors of our university students，to provide some scientific basis to develop the autonomous learning theory. Methods We collected the data by stratified cluster sampling between 2010 and 2011 grade of certain Medical University students in 5 majors. And the binary Logistic regression was used to analyze the influencing factors. Results（1）79.5% in 519 students has carried out autonomous learning，but 70.42% of students thought autonomous learning not a good learning method. （2）Just 44.3% students indicated that they had got the effective guidance about autonomous learning from teacher. （3）Students who with learning plan were more likely（1.981 times）to carry out autonomous learning than who without learning plan. Conclusion Making a reasonable study plan can guide students to study independently.
2016, 37(01)
Abstract:
［Abstract］Objective To discuss the application of open experiment teaching in practice of teaching Preventive Medicine in a Medical University. Methods At first，the baseline questionnaires were completed for 195 students in grade 2010 of preventive medicine. Then we carried out an open experiment teaching （food poisoning）. At last， the final stage investigation was given. Then we compared the 2 survey results and analysis of students' understanding of open experimental teaching. Results The 2 survey results showed that more than 80% students believed that it was necessaty to improve the experimental teaching of preventive medicine，more than 65% students thought open experimental teaching improved students' comprehensive ability of students；more than 60% of the students were in favor of carrying out open experiment teaching. Conclusions Experimental course is an important way way to develop the students' comprehensive ability， the traditional experiment teaching method is limited in cultivating students' innovation ability．The open experimental teaching reform is an important way to improve the preventive medicine experimental teaching effect， to improved the students' learning enthusiasm and subjective.
［Abstract］Objective To discuss the application of open experiment teaching in practice of teaching Preventive Medicine in a Medical University. Methods At first，the baseline questionnaires were completed for 195 students in grade 2010 of preventive medicine. Then we carried out an open experiment teaching （food poisoning）. At last， the final stage investigation was given. Then we compared the 2 survey results and analysis of students' understanding of open experimental teaching. Results The 2 survey results showed that more than 80% students believed that it was necessaty to improve the experimental teaching of preventive medicine，more than 65% students thought open experimental teaching improved students' comprehensive ability of students；more than 60% of the students were in favor of carrying out open experiment teaching. Conclusions Experimental course is an important way way to develop the students' comprehensive ability， the traditional experiment teaching method is limited in cultivating students' innovation ability．The open experimental teaching reform is an important way to improve the preventive medicine experimental teaching effect， to improved the students' learning enthusiasm and subjective.
2016, 37(01)
Abstract:
［Abstract］Objective To observe the effect of high quality nursing care on the negative emotion and treatment compliance of patients with leukemia. Methods 116 patients with leukemia in our hospital from January 2010 to April 2014 were randomly divided into study group and control group，58 cases in each group. The control group was given routine nursing measures， and the research group was given high quality care measures based on the control group. Self rating Depression Scale（SDS）and self rating Anxiety Scale（SAS）was used to evaluate the adverse mood changes between the two groups before and after the intervention，and the treatment compliance， nursing satisfaction and the incidence of complications of the two groups were compared. Results After the intervention， SDS and SAS scores of the study group were significantly lower than those of the control group after the intervention， the difference was statistically significant（P＜0.05）.The treatment compliance and satisfaction with nursing care of the study group were improved significantly than those of the control group（87.93% vs 68.97%，96.55% vs 81.03%），the difference was statistically significant（P＜0.05）.The complication rate of the study group decreased significantly than that of the control group（15.52% vs 31.03%），the difference was statistically significant（P＜0.05）. Conclusion High quality nursing for patients with leukemia can significantly improve the psychological status of patients， improve the treatment compliance and nursing satisfaction，the incidence of complications decreased significantly.
［Abstract］Objective To observe the effect of high quality nursing care on the negative emotion and treatment compliance of patients with leukemia. Methods 116 patients with leukemia in our hospital from January 2010 to April 2014 were randomly divided into study group and control group，58 cases in each group. The control group was given routine nursing measures， and the research group was given high quality care measures based on the control group. Self rating Depression Scale（SDS）and self rating Anxiety Scale（SAS）was used to evaluate the adverse mood changes between the two groups before and after the intervention，and the treatment compliance， nursing satisfaction and the incidence of complications of the two groups were compared. Results After the intervention， SDS and SAS scores of the study group were significantly lower than those of the control group after the intervention， the difference was statistically significant（P＜0.05）.The treatment compliance and satisfaction with nursing care of the study group were improved significantly than those of the control group（87.93% vs 68.97%，96.55% vs 81.03%），the difference was statistically significant（P＜0.05）.The complication rate of the study group decreased significantly than that of the control group（15.52% vs 31.03%），the difference was statistically significant（P＜0.05）. Conclusion High quality nursing for patients with leukemia can significantly improve the psychological status of patients， improve the treatment compliance and nursing satisfaction，the incidence of complications decreased significantly.
