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RSS2016 Vol. 37, No. 03
2016, 37(03)
Abstract:
［Abstract］Male breast cancer（MBC）is a rare disease. In recent years, although MBC accounts for a small part of all diagnosed breast cancer, its incidence continues to rise. Based on its small incidence，the researches for MBC are much less than female breast cancer（FBC）. This article will review the etiology，clinical and pathological characterastics，diagnosis，treatment and prognosis of MBC.
［Abstract］Male breast cancer（MBC）is a rare disease. In recent years, although MBC accounts for a small part of all diagnosed breast cancer, its incidence continues to rise. Based on its small incidence，the researches for MBC are much less than female breast cancer（FBC）. This article will review the etiology，clinical and pathological characterastics，diagnosis，treatment and prognosis of MBC.
2016, 37(03)
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［Abstract］Objective To study the expression characteristics of PCNA， NOXA， PUMA and MDM2 in breast cancer. Methods The cancer tissue and adjacent normal tissue were collected from 35 patients with breast cancer. The total RNA was extracted using TRIzol and the expression levels of MDM2， PCNA， NOXA and PUMA were detected by real-time quantitative PCR. Results NOXA and PUMA levels in cancer tissue were significantly higher than those in adjacent normal tissue（P < 0.05）. There were significant positive correlations between the expressions of PCNA and NOXA （r＝0.82， P < 0.01）， PCNA and MDM2 （r＝0.80， P < 0.01）， and PUMA and MDM2（r＝0.82，P < 0.01）in adjacent normal tissue. Conclusion The levels of NOXA and PUMA were high expression in cancer tissue， and the expressions of all 4 genes showed relations with breast cancer.
［Abstract］Objective To study the expression characteristics of PCNA， NOXA， PUMA and MDM2 in breast cancer. Methods The cancer tissue and adjacent normal tissue were collected from 35 patients with breast cancer. The total RNA was extracted using TRIzol and the expression levels of MDM2， PCNA， NOXA and PUMA were detected by real-time quantitative PCR. Results NOXA and PUMA levels in cancer tissue were significantly higher than those in adjacent normal tissue（P < 0.05）. There were significant positive correlations between the expressions of PCNA and NOXA （r＝0.82， P < 0.01）， PCNA and MDM2 （r＝0.80， P < 0.01）， and PUMA and MDM2（r＝0.82，P < 0.01）in adjacent normal tissue. Conclusion The levels of NOXA and PUMA were high expression in cancer tissue， and the expressions of all 4 genes showed relations with breast cancer.
2016, 37(03)
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［Abstract］Objective To investigate the change of long-term behavior， brain morphology and myelin basic protein （MBP） expression after systematic hypoxia exposure in P0d neonatal rats. Methods P0d neonatal SD rats were randomly and equally divided into control group（NG group）and systematic hypoxia group（Hy group）. Hy group rats were exposed to 5%O2 for 3.5h. Behavior tests were applied at P29d and P30d. 1.5T MRI scanning of brain was used to detect morphology change in P60d rats after hypoxia. The changes in ultrastructure of hippocampal CA1 and corpus callosum（CC）after hypoxia were further confirmed by transmission electron microscopy（TEM）. Immunoblotting assays were performed to identify the dynamic changes of MBP. Results（1）The scores in refused captured test，open field test，slope experiments and the suspension test decreased（P < 0.05）. The success rates of Y arm maze test decreased and days up to standard significantly prolonged （P < 0.001）. （2）Abnormal signal in hippocampus and lateral ventricle， thinner corpus callosum were observed by 1.5T MRI scanning after hypoxia 2 months. （3）Electron microscope results revealed loosen structure and neuropil edema appeared in periventricular white matter. （4）The expression of MBP in the periventricular regions of NG group was detected by immunoblotting assay at the early development stages. Systemic hypoxia exposure to neonatal rats could decrease MBP expression after hypoxia 3 days but increase expression after hypoxia 4 hours（P < 0.05）. Conclusion An obviously morphology injury which distinct located in periventricular white matter（PWM）and the abnormal of the long-term behavior can be confirmed after systematic hypoxia exposure in neonatal rats.
［Abstract］Objective To investigate the change of long-term behavior， brain morphology and myelin basic protein （MBP） expression after systematic hypoxia exposure in P0d neonatal rats. Methods P0d neonatal SD rats were randomly and equally divided into control group（NG group）and systematic hypoxia group（Hy group）. Hy group rats were exposed to 5%O2 for 3.5h. Behavior tests were applied at P29d and P30d. 1.5T MRI scanning of brain was used to detect morphology change in P60d rats after hypoxia. The changes in ultrastructure of hippocampal CA1 and corpus callosum（CC）after hypoxia were further confirmed by transmission electron microscopy（TEM）. Immunoblotting assays were performed to identify the dynamic changes of MBP. Results（1）The scores in refused captured test，open field test，slope experiments and the suspension test decreased（P < 0.05）. The success rates of Y arm maze test decreased and days up to standard significantly prolonged （P < 0.001）. （2）Abnormal signal in hippocampus and lateral ventricle， thinner corpus callosum were observed by 1.5T MRI scanning after hypoxia 2 months. （3）Electron microscope results revealed loosen structure and neuropil edema appeared in periventricular white matter. （4）The expression of MBP in the periventricular regions of NG group was detected by immunoblotting assay at the early development stages. Systemic hypoxia exposure to neonatal rats could decrease MBP expression after hypoxia 3 days but increase expression after hypoxia 4 hours（P < 0.05）. Conclusion An obviously morphology injury which distinct located in periventricular white matter（PWM）and the abnormal of the long-term behavior can be confirmed after systematic hypoxia exposure in neonatal rats.
2016, 37(03)
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［Abstract］Objective To establish a rapid and specific quantitative PCR assay for detecting of ACACB gene rs2268388 polymorphism. Methods The primers and Taqman probe targeted at signature sequence of ACACB gene rs2268388 polymorphism were designed and applied to real time PCR assays. The different concentrations were set up， and the sensitivity and specificity were evaluated. Results Initial denaturation time of PCR was 10 min， annealing and extending time were 1 min， probe concentration of Taqman PCR was 0.5 μL（0.2 μM），TaqMan Genotyping Master Mix was 5 μL， dH2O was 2.5 μL，and total volume was 10 μL. At this condition，the result of ACACB gene rs2268388 polymorphism showed the high specificity of the reaction. Conclusion The assay was sensitive and specific for rapid identification and quantitative detection of ACACB gene rs2268388 polymorphism. It will be an effective method for the surveillance and diagnosis of ACACB gene rs2268388 polymorphism.
［Abstract］Objective To establish a rapid and specific quantitative PCR assay for detecting of ACACB gene rs2268388 polymorphism. Methods The primers and Taqman probe targeted at signature sequence of ACACB gene rs2268388 polymorphism were designed and applied to real time PCR assays. The different concentrations were set up， and the sensitivity and specificity were evaluated. Results Initial denaturation time of PCR was 10 min， annealing and extending time were 1 min， probe concentration of Taqman PCR was 0.5 μL（0.2 μM），TaqMan Genotyping Master Mix was 5 μL， dH2O was 2.5 μL，and total volume was 10 μL. At this condition，the result of ACACB gene rs2268388 polymorphism showed the high specificity of the reaction. Conclusion The assay was sensitive and specific for rapid identification and quantitative detection of ACACB gene rs2268388 polymorphism. It will be an effective method for the surveillance and diagnosis of ACACB gene rs2268388 polymorphism.
2016, 37(03)
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［Abstract］Objective The study was aimed to investigate the diversity of endophytic fungi from Ligustrum vicaryi and their antibacterial activity. Methods Endophytic fungi were isolated by the method of explant culture from tissue segments and then cultured in YPD liquid medium，respectively. The fermentation broth and mycelia were extracted with acetone after filtration. Escherichia coli， Pseudomonas aeruginosa and Staphylococcus aureus were selected as indicators. The extracts were tested by filtering paper method. Results The results indicated that 29 strains were isolated from L. vicaryi，and 25 of them were found to have a certain antibacterial activity（86.2% of the total strains isolated）. The other 4 strains were considered inactive（17.2% of the total strains isolated）. Conclusion There were abundant endophytic fungi in the stem and leaf of L. vicaryi. Their secondary metabolites showed significant antibacterial activity.
［Abstract］Objective The study was aimed to investigate the diversity of endophytic fungi from Ligustrum vicaryi and their antibacterial activity. Methods Endophytic fungi were isolated by the method of explant culture from tissue segments and then cultured in YPD liquid medium，respectively. The fermentation broth and mycelia were extracted with acetone after filtration. Escherichia coli， Pseudomonas aeruginosa and Staphylococcus aureus were selected as indicators. The extracts were tested by filtering paper method. Results The results indicated that 29 strains were isolated from L. vicaryi，and 25 of them were found to have a certain antibacterial activity（86.2% of the total strains isolated）. The other 4 strains were considered inactive（17.2% of the total strains isolated）. Conclusion There were abundant endophytic fungi in the stem and leaf of L. vicaryi. Their secondary metabolites showed significant antibacterial activity.
2016, 37(03)
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［Abstract］Objective To analyze the liver function changes of type 2 diabetes mellitus rat model induced by high fat diet and streptozotocin（STZ）. Methods 65 male SD rats were randomly divided into control group （20 rats） and high-fat group（45 rats）. Rats in the two groups were fed normal diet and high-fat diet， respectively. After eight weeks feeding，eight rats were selected from high-fat group randomly and given different amount of STZ. Finally，30 mg/kg was identified as the most suitable dosage for induced the rats into type 2 diabetes mellitus. The remaining 45 high-fat group rats was injected 30mg/kg by intraperitoneal injection and then 20 rats were randomly selected from successfully established type 2 diabetes mellitus rats. Serum and liver were obtained when rats were killed after four and eight weeks. Results Rats with type 2 diabetes mellitus showed obvious polyuria， polydipsia， polyphagia and weight loss. Their blood glucose levels were higher than 11.1 mmol/L，and the insulin sensitive index at the forth week decreased more obvious than the eighth week. Compare with the control group， liver cell disorder and vesicular fat lesions was found in the type 2 diabetic rats through the liver hematoxylin-eosin staining，the serum triglycerides（TG） increased and TG at the fourth week was obviously lower than that at the eighth week. In addition，cholesterol（TC），bile acid（TBA），Bilirubin（TBIL）， alkaline phosphatase （AKP）and alanine aminotransferase（ALT）of rats with type 2 diabetes mellitus were significantly higher than those of the control group，with statistical significances（P < 0.05），especially at the eighth week. Conclusion Rats with type 2 diabetes mellitus induced by high fat diet and STZ might have fatty liver and biliary dyskinesia.
［Abstract］Objective To analyze the liver function changes of type 2 diabetes mellitus rat model induced by high fat diet and streptozotocin（STZ）. Methods 65 male SD rats were randomly divided into control group （20 rats） and high-fat group（45 rats）. Rats in the two groups were fed normal diet and high-fat diet， respectively. After eight weeks feeding，eight rats were selected from high-fat group randomly and given different amount of STZ. Finally，30 mg/kg was identified as the most suitable dosage for induced the rats into type 2 diabetes mellitus. The remaining 45 high-fat group rats was injected 30mg/kg by intraperitoneal injection and then 20 rats were randomly selected from successfully established type 2 diabetes mellitus rats. Serum and liver were obtained when rats were killed after four and eight weeks. Results Rats with type 2 diabetes mellitus showed obvious polyuria， polydipsia， polyphagia and weight loss. Their blood glucose levels were higher than 11.1 mmol/L，and the insulin sensitive index at the forth week decreased more obvious than the eighth week. Compare with the control group， liver cell disorder and vesicular fat lesions was found in the type 2 diabetic rats through the liver hematoxylin-eosin staining，the serum triglycerides（TG） increased and TG at the fourth week was obviously lower than that at the eighth week. In addition，cholesterol（TC），bile acid（TBA），Bilirubin（TBIL）， alkaline phosphatase （AKP）and alanine aminotransferase（ALT）of rats with type 2 diabetes mellitus were significantly higher than those of the control group，with statistical significances（P < 0.05），especially at the eighth week. Conclusion Rats with type 2 diabetes mellitus induced by high fat diet and STZ might have fatty liver and biliary dyskinesia.
2016, 37(03)
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［Abstract］Objective To investigate the effect of the fibrin glue（FG）as a carrier compounded with BMP-2 on repairing bone defect and stimulating early period bone formation，and to provide the experimental foundation for the clinical application. Methods The fibrin glue compounded with growth factor BMP-2 delivery system was prepared with the consistency of BMP-21 μg/mL. Bone defects（8.00 mm length，2.00mm width and 1.00mm depth）were created in 36 rabbits on the bilateral radius and rabbits were divided into three groups. They were treated with three kinds of implantations，including fibrin compounded with BMP-2 in experimental group，fibrin in control group and no material in blank control group. Rabbits were sacrificed at the end of 2 weeks，4 weeks and 8 weeks after operation，respectively. Then specimens were obtained in each time point and bone formation and osteocalcin（OC）were detected by X-ray，histological section and immunohistochemical method. Results The bone forming activity and bone healing speed in experimental group were significantly better than those of the other groups by X-ray and histological section. Immunohistochemical results showed OC contents were highest and there were significant differences among the three groups in the second week（P < 0.01）. In experimental group， the expression amount of OC decreased in local bone and bone callus organization， and the most significant differences among the three groups appeared at the fourth week（P < 0.01）. There was no difference in the expression of OC between the experimental group and the other groups at the eighth week（P > 0.05）. Conclusion The material with fibrin glue as a carrier compounded with BMP-2 could significantly accelerate the new bone forming， regeneration，mineralization and remodeling，shorten the recovery period，which can provide a clinical method for repairing bone defect.
［Abstract］Objective To investigate the effect of the fibrin glue（FG）as a carrier compounded with BMP-2 on repairing bone defect and stimulating early period bone formation，and to provide the experimental foundation for the clinical application. Methods The fibrin glue compounded with growth factor BMP-2 delivery system was prepared with the consistency of BMP-21 μg/mL. Bone defects（8.00 mm length，2.00mm width and 1.00mm depth）were created in 36 rabbits on the bilateral radius and rabbits were divided into three groups. They were treated with three kinds of implantations，including fibrin compounded with BMP-2 in experimental group，fibrin in control group and no material in blank control group. Rabbits were sacrificed at the end of 2 weeks，4 weeks and 8 weeks after operation，respectively. Then specimens were obtained in each time point and bone formation and osteocalcin（OC）were detected by X-ray，histological section and immunohistochemical method. Results The bone forming activity and bone healing speed in experimental group were significantly better than those of the other groups by X-ray and histological section. Immunohistochemical results showed OC contents were highest and there were significant differences among the three groups in the second week（P < 0.01）. In experimental group， the expression amount of OC decreased in local bone and bone callus organization， and the most significant differences among the three groups appeared at the fourth week（P < 0.01）. There was no difference in the expression of OC between the experimental group and the other groups at the eighth week（P > 0.05）. Conclusion The material with fibrin glue as a carrier compounded with BMP-2 could significantly accelerate the new bone forming， regeneration，mineralization and remodeling，shorten the recovery period，which can provide a clinical method for repairing bone defect.
2016, 37(03)
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［Abstract］Objective To investigate the drug resistance of clinical isolated Pseudomonas aeruginosa（PA） and the genotypes of extended-spectrum β-lactamases（ESBLs）produced by them in Kunming. Methods 125 clinical isolates of PA were collected from 4 hospitals in Kunming and identified with VITEK-32 automated bacteria identification instrument. The Kirby-Bauer method was used for antimicrobial susceptibility to 20 kinds of antibiotics. And polymerase chain reaction（PCR）analysis was used to detect 7 kinds of ESBLs genes including TEM，SHV， OXA，PER，VEB，GES and CTX-M-1. DNA sequencing was used to detect the positive production of PCR with GenBank as control. Results The resistance rates of 125 isolated PA were high to beta-lactam antibiotics，but low to aminoglycosides antibiotics. The detection rates of ESBLs genes were all above 90% from 4 hospitals in Kunming， and 7 kinds of ESBLs genes were all detected. Among the 7 kinds of ESBLs genes，the detection rate of TEM was 76%. Conclusion The detection rates of ESBLs genes were high in Kunming，and the distribution of ESBLs genotypes was varied. TEM was a main genotype among prevalent ESBLs genotypes. For local hospitals， attention must be paid to reasonable use of antibiotics，and should strengthen laboratories associated surveillance dynamically.
［Abstract］Objective To investigate the drug resistance of clinical isolated Pseudomonas aeruginosa（PA） and the genotypes of extended-spectrum β-lactamases（ESBLs）produced by them in Kunming. Methods 125 clinical isolates of PA were collected from 4 hospitals in Kunming and identified with VITEK-32 automated bacteria identification instrument. The Kirby-Bauer method was used for antimicrobial susceptibility to 20 kinds of antibiotics. And polymerase chain reaction（PCR）analysis was used to detect 7 kinds of ESBLs genes including TEM，SHV， OXA，PER，VEB，GES and CTX-M-1. DNA sequencing was used to detect the positive production of PCR with GenBank as control. Results The resistance rates of 125 isolated PA were high to beta-lactam antibiotics，but low to aminoglycosides antibiotics. The detection rates of ESBLs genes were all above 90% from 4 hospitals in Kunming， and 7 kinds of ESBLs genes were all detected. Among the 7 kinds of ESBLs genes，the detection rate of TEM was 76%. Conclusion The detection rates of ESBLs genes were high in Kunming，and the distribution of ESBLs genotypes was varied. TEM was a main genotype among prevalent ESBLs genotypes. For local hospitals， attention must be paid to reasonable use of antibiotics，and should strengthen laboratories associated surveillance dynamically.
2016, 37(03)
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［Abstract］Objective To investigate the depression status and influencing factors for the late pregnancy women， and provide a theoretical basis for psychological consultations and interventions. Methods A symptoms of depression self rating scale（SDS）and a questionnaire for the basic information were adopted in the cross-sectional survey for 180 late pregnancy women at 35 weeks or more in the obstetrics of the second affiliated hospital of Kunming Medical University from March to May in 2015. Logistic regression analysis was used to explore the related risk factors of depression. Results The depression positive rate of late pregnancy women was 42.2%，among which the depression positive rate of the Han nationality（47.45%）was obviously higher than national minority's （25.58%），and Bachelor degree or above （52.94%） was higher than Bachelor degree and below（35.71%）. Logistic regression analysis showed that the risk factors for depression symptoms were nationality（OR=2.92，95%CI: 1.29-6.63），education（OR=3.29， 95%CI: 1.22-7.18），satisfaction degree of current marital status （OR=3.53， 95%CI: 1.09-6.24），and sleep time of every day（OR=0.37，95%CI:0.15-0.94）. Conclusion The present mental health status of the late pregnancy women in Kunming was poor，especially the pregnant women with Bachelor degree or above and poor quality of sleep，who were prone to depression. Extensive health education should be launched to pregnant women in order to reduce the possibility of depression during pregnancy.
［Abstract］Objective To investigate the depression status and influencing factors for the late pregnancy women， and provide a theoretical basis for psychological consultations and interventions. Methods A symptoms of depression self rating scale（SDS）and a questionnaire for the basic information were adopted in the cross-sectional survey for 180 late pregnancy women at 35 weeks or more in the obstetrics of the second affiliated hospital of Kunming Medical University from March to May in 2015. Logistic regression analysis was used to explore the related risk factors of depression. Results The depression positive rate of late pregnancy women was 42.2%，among which the depression positive rate of the Han nationality（47.45%）was obviously higher than national minority's （25.58%），and Bachelor degree or above （52.94%） was higher than Bachelor degree and below（35.71%）. Logistic regression analysis showed that the risk factors for depression symptoms were nationality（OR=2.92，95%CI: 1.29-6.63），education（OR=3.29， 95%CI: 1.22-7.18），satisfaction degree of current marital status （OR=3.53， 95%CI: 1.09-6.24），and sleep time of every day（OR=0.37，95%CI:0.15-0.94）. Conclusion The present mental health status of the late pregnancy women in Kunming was poor，especially the pregnant women with Bachelor degree or above and poor quality of sleep，who were prone to depression. Extensive health education should be launched to pregnant women in order to reduce the possibility of depression during pregnancy.
2016, 37(03)
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［Abstract］Objective To explore the effects of yoga on undergraduate physical function and quality. Methods 48 students in university were selected as the experimental objects with 12 weeks of exercise prescription intervention， including 24 students of experimental group with prescription intervention of yoga exercise and 24 students of control group with freedom movement of other sports. Before and after the experiment， the body quality and function indexes of students were detected and analyzed using the national physique health monitoring standards. Results Yoga exercise prescription intervention showed an obvious effect. After the intervention，the heart rate at rest， vital capacity and standing long jump were significantly higher than those before the intervention in the experimental group （P < 0.05）， and the other indexes were also significantly improved （P < 0.01）. Although the indexes in the control group were improved after intervention， there were no statistical differences. Conclusion Yoga has a significant influence on college students' physiological functions and can improve the physical quality significantly， which plays a positive role on the improvement of the college student's physical fitness.
［Abstract］Objective To explore the effects of yoga on undergraduate physical function and quality. Methods 48 students in university were selected as the experimental objects with 12 weeks of exercise prescription intervention， including 24 students of experimental group with prescription intervention of yoga exercise and 24 students of control group with freedom movement of other sports. Before and after the experiment， the body quality and function indexes of students were detected and analyzed using the national physique health monitoring standards. Results Yoga exercise prescription intervention showed an obvious effect. After the intervention，the heart rate at rest， vital capacity and standing long jump were significantly higher than those before the intervention in the experimental group （P < 0.05）， and the other indexes were also significantly improved （P < 0.01）. Although the indexes in the control group were improved after intervention， there were no statistical differences. Conclusion Yoga has a significant influence on college students' physiological functions and can improve the physical quality significantly， which plays a positive role on the improvement of the college student's physical fitness.
2016, 37(03)
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［Abstract］Objective To explore the clinical significance of white blood cell（WBC），C-reactive protein （CRP），Erythrocyte sedimentation rate（ESR），serum amyloid A（SAA），procalcitonin （PCT）and neutrophil granulocyte percentage（GR%）in patients with knee infection. Methods A retrospective study was conducted from March 2012 to January 2015. One hundred and eighty-five patients were collected in the study. They were divided into knee infection group（A，37 patients） and non-infection group（B，148 patients） according to the results of postoperative blood culture. The values of WBC， CRP， ESR， SAA，PCT and GR% were compared and analyzed between the two groups. Results The values of the 6 indicators in group A were higher than those in group B before operation，with statistically significant differences（P < 0.05）. The values of WBC， CRP，ESR and SAA in group A were higher than those in group B on post-operation day 1（P < 0.05）. However，PCT and GR% were no statistically significance between groups A and B（P > 0.05）. The values of 6 indicators on post-operation days 3 and 7 were no statistically significance between the two groups（P > 0.05）. Conclusion WBC，CRP，ESR，SAA，PCT and GR% showed good reference values for early diagnosis and prevention of infection，which can be used as the screen testing indexes in the early knee infection.
［Abstract］Objective To explore the clinical significance of white blood cell（WBC），C-reactive protein （CRP），Erythrocyte sedimentation rate（ESR），serum amyloid A（SAA），procalcitonin （PCT）and neutrophil granulocyte percentage（GR%）in patients with knee infection. Methods A retrospective study was conducted from March 2012 to January 2015. One hundred and eighty-five patients were collected in the study. They were divided into knee infection group（A，37 patients） and non-infection group（B，148 patients） according to the results of postoperative blood culture. The values of WBC， CRP， ESR， SAA，PCT and GR% were compared and analyzed between the two groups. Results The values of the 6 indicators in group A were higher than those in group B before operation，with statistically significant differences（P < 0.05）. The values of WBC， CRP，ESR and SAA in group A were higher than those in group B on post-operation day 1（P < 0.05）. However，PCT and GR% were no statistically significance between groups A and B（P > 0.05）. The values of 6 indicators on post-operation days 3 and 7 were no statistically significance between the two groups（P > 0.05）. Conclusion WBC，CRP，ESR，SAA，PCT and GR% showed good reference values for early diagnosis and prevention of infection，which can be used as the screen testing indexes in the early knee infection.
2016, 37(03)
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［Abstract］Objective To investigate the preventive effects of azasetron against postoperative nausea and vomiting（PONV）after laparoscopic cholecystectomy in female patients at menstrual phase. Methods Forty ASA Ⅰ or Ⅱ female patients at menstrual phase were randomly divided into azasetron group（n＝20）and control group （n＝20）. Induction and maintenance of anesthesia was routine. Azasetron 10mg or normal saline 50 ml was given at 30 min before the end of surgery. The incidence and intensity of PONV was recorded during 48 hours after surgery. Results Compare to the control group, the incidence of PONV was lower at 48 hour after surgery in azasetron group （35% vs 90%，P < 0.01），and the incidence of PONV in Ⅳ level was also lower in azasetron group（P < 0.05）. Conclusion Azasetron can decrease the incidence and intensity of PONV after laparoscopic cholecystectomy during menstrual phase.
［Abstract］Objective To investigate the preventive effects of azasetron against postoperative nausea and vomiting（PONV）after laparoscopic cholecystectomy in female patients at menstrual phase. Methods Forty ASA Ⅰ or Ⅱ female patients at menstrual phase were randomly divided into azasetron group（n＝20）and control group （n＝20）. Induction and maintenance of anesthesia was routine. Azasetron 10mg or normal saline 50 ml was given at 30 min before the end of surgery. The incidence and intensity of PONV was recorded during 48 hours after surgery. Results Compare to the control group, the incidence of PONV was lower at 48 hour after surgery in azasetron group （35% vs 90%，P < 0.01），and the incidence of PONV in Ⅳ level was also lower in azasetron group（P < 0.05）. Conclusion Azasetron can decrease the incidence and intensity of PONV after laparoscopic cholecystectomy during menstrual phase.
2016, 37(03)
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［Abstract］Objective To compare the clinical efficacy between transurethral plasmakinetic enucleation of prostate（PKEP） and holmium laser enucleation of the prostate （HoLEP） in the treatment of benign prostatic hyperplasia（BPH）. Methods 90 patients who were diagnosed BPH were randomly and equally divided into PKEP group and HoLEP group. The perioperative and postoperative indexes after 6 months were recorded and analyzed. Results There were no significance differences in preoperative factors between the two groups （P > 0.05）. The operative time in HoLEP group was longer than that in PKEP group， but the incidence of hemorrhage， the mean bladder irrigating time， catheter indwelling time and hospital stay time in HoLEP were significant shorter or less than those in PKEP（P < 0.01）. After 6 months，PVR，IPSS，Qmax and QOL were both significantly improved（P < 0.01）， but the differences between the two groups were not significant （P > 0.05）. The complications in HoLEP were less than those in PKEP（P < 0.05）. Conclusion HoLEP and PKEP have the same short-term curative effect in the treatment of BPH， but the former is more security with fewer complications. HoLEP is worthy of widely application.
［Abstract］Objective To compare the clinical efficacy between transurethral plasmakinetic enucleation of prostate（PKEP） and holmium laser enucleation of the prostate （HoLEP） in the treatment of benign prostatic hyperplasia（BPH）. Methods 90 patients who were diagnosed BPH were randomly and equally divided into PKEP group and HoLEP group. The perioperative and postoperative indexes after 6 months were recorded and analyzed. Results There were no significance differences in preoperative factors between the two groups （P > 0.05）. The operative time in HoLEP group was longer than that in PKEP group， but the incidence of hemorrhage， the mean bladder irrigating time， catheter indwelling time and hospital stay time in HoLEP were significant shorter or less than those in PKEP（P < 0.01）. After 6 months，PVR，IPSS，Qmax and QOL were both significantly improved（P < 0.01）， but the differences between the two groups were not significant （P > 0.05）. The complications in HoLEP were less than those in PKEP（P < 0.05）. Conclusion HoLEP and PKEP have the same short-term curative effect in the treatment of BPH， but the former is more security with fewer complications. HoLEP is worthy of widely application.
2016, 37(03)
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［Abstract］Objective To summarize the clinical experience of the division under the thoracoscope esophagus cancer radical and appreciate，evaluate its recent curative effect．Methods Our department were retrospectively analyzed in August 2013 to March 2015 between thoracoscope the clinical data of 25 cases with esophageal cancer radical．Results This group of patients with no perioperative death， transit thoracotomy in 3 patients.Thoracoscope surgery group the average number of lymph node cleaning more（13.8±3.4）/cases: （11.1± 11.1）/cases;Chest surgery less blood loss （160.5±24） ml: ml （290.5±36.5）; Shorter postoperative chest tube drawing tube （3.9±1.5）， d （4.9±2.4） d; Pulmonary complications（7.3%） less （15.6%）；Overall a shorter hospital stay（12.3±3.5） d: （15.4±5.6） d；But the chest surgery longer（125±43.5） min/example: minf/cases（60.5-30.6）．Conclusion Cavity mirror as a new type of esophageal cancer radical surgery minimally invasive techniques，due to its for a surgeon， equipment and technical requirement is high， in the clinical development process of twists and turns，its various advantages make up for the deficiency of the esophageal cancer radical open completely，in conditions allow clinical surgery should be widely applied. Compared with traditional three incisions under thoracoscope esophagus cancer resection， with lymph node cleaning thoroughly， small trauma，less bleeding，fewer complications， quick recovery， treatment effect is satisfactory.
［Abstract］Objective To summarize the clinical experience of the division under the thoracoscope esophagus cancer radical and appreciate，evaluate its recent curative effect．Methods Our department were retrospectively analyzed in August 2013 to March 2015 between thoracoscope the clinical data of 25 cases with esophageal cancer radical．Results This group of patients with no perioperative death， transit thoracotomy in 3 patients.Thoracoscope surgery group the average number of lymph node cleaning more（13.8±3.4）/cases: （11.1± 11.1）/cases;Chest surgery less blood loss （160.5±24） ml: ml （290.5±36.5）; Shorter postoperative chest tube drawing tube （3.9±1.5）， d （4.9±2.4） d; Pulmonary complications（7.3%） less （15.6%）；Overall a shorter hospital stay（12.3±3.5） d: （15.4±5.6） d；But the chest surgery longer（125±43.5） min/example: minf/cases（60.5-30.6）．Conclusion Cavity mirror as a new type of esophageal cancer radical surgery minimally invasive techniques，due to its for a surgeon， equipment and technical requirement is high， in the clinical development process of twists and turns，its various advantages make up for the deficiency of the esophageal cancer radical open completely，in conditions allow clinical surgery should be widely applied. Compared with traditional three incisions under thoracoscope esophagus cancer resection， with lymph node cleaning thoroughly， small trauma，less bleeding，fewer complications， quick recovery， treatment effect is satisfactory.
2016, 37(03)
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［Abstract］Objective The study was aimed to explore the clinical application of iodine oil-docetaxel－suspension in transcatheter arterial chemoembolization （TACE） for primary hepatic carcinoma and find out more effective treatment of TACE to provide a guidance for clinical treatment. Methods 52 patients who were diagnosed with primary hepatic carcinoma during October 2012 to August 2014 were randomly divided into groups A and B， including 32 male and 20 female cases. Group A was given iodine oil-docetaxel-suspension and gemcitabine treatment and group B received iodine oil-epirubicin-suspension and gemcitabine treatment. A comparison was conducted in the curative effect， liver function， quality of life， KPS score， AFP changes， the cumulative survival and other changes of patients between the two groups. Results There were no significant differences in gender， age， HBsAg （+）and the Child-Pugh score of 52 cases（P > 0.05）. The disease control rate in groups A and B was 21.8% （7 cases）and 20% （4 cases）， and there was no significant difference between the two groups（P > 0.05）. The tumor response rate in groups A and B was 84.3% （27 cases） and 80% （16 cases）， with no significant difference（P > 0.05）. There were no significant differences in PR，SD and PD between the two groups （P > 0.05）. Postoperative serum AFP of patients in the two groups was significantly decreased compared with preoperative （P < 0.05）. The mean survival time and median survival period of group A were 14.4 and 13.7 months， while those of group B were 12.1 and 9.7 months，respectively. There were no significant differences in 6 and 24 months survival rates between the two groups（P > 0.05）， but there were significant differences in 12 and 18 months（P < 0.05）. Compared with group B，liver function was improved in group A after TACE， with a significant difference（P < 0.05）. In the quality of life， KPS scores of two groups increased gradually within 3 months after TACE，but reduced after 3 months. The KPS scores of group A were higher than those of group B on 1， 6 and 12 months after TACE（P < 0.05）. Conclusion The interventional therapy of two groups are both effective in treating primary hepatic carcinoma. But iodine oil-docetaxel-suspension TACE for primary hepatic carcinoma has advantages in improving the quality of life， prolonging the survival time，reducing the damage of liver function，and inhibiting tumor cell growth，whicht is worthy of clinical promotion.
［Abstract］Objective The study was aimed to explore the clinical application of iodine oil-docetaxel－suspension in transcatheter arterial chemoembolization （TACE） for primary hepatic carcinoma and find out more effective treatment of TACE to provide a guidance for clinical treatment. Methods 52 patients who were diagnosed with primary hepatic carcinoma during October 2012 to August 2014 were randomly divided into groups A and B， including 32 male and 20 female cases. Group A was given iodine oil-docetaxel-suspension and gemcitabine treatment and group B received iodine oil-epirubicin-suspension and gemcitabine treatment. A comparison was conducted in the curative effect， liver function， quality of life， KPS score， AFP changes， the cumulative survival and other changes of patients between the two groups. Results There were no significant differences in gender， age， HBsAg （+）and the Child-Pugh score of 52 cases（P > 0.05）. The disease control rate in groups A and B was 21.8% （7 cases）and 20% （4 cases）， and there was no significant difference between the two groups（P > 0.05）. The tumor response rate in groups A and B was 84.3% （27 cases） and 80% （16 cases）， with no significant difference（P > 0.05）. There were no significant differences in PR，SD and PD between the two groups （P > 0.05）. Postoperative serum AFP of patients in the two groups was significantly decreased compared with preoperative （P < 0.05）. The mean survival time and median survival period of group A were 14.4 and 13.7 months， while those of group B were 12.1 and 9.7 months，respectively. There were no significant differences in 6 and 24 months survival rates between the two groups（P > 0.05）， but there were significant differences in 12 and 18 months（P < 0.05）. Compared with group B，liver function was improved in group A after TACE， with a significant difference（P < 0.05）. In the quality of life， KPS scores of two groups increased gradually within 3 months after TACE，but reduced after 3 months. The KPS scores of group A were higher than those of group B on 1， 6 and 12 months after TACE（P < 0.05）. Conclusion The interventional therapy of two groups are both effective in treating primary hepatic carcinoma. But iodine oil-docetaxel-suspension TACE for primary hepatic carcinoma has advantages in improving the quality of life， prolonging the survival time，reducing the damage of liver function，and inhibiting tumor cell growth，whicht is worthy of clinical promotion.
2016, 37(03)
Abstract:
［Abstract］Objective The study was aimed to analyze the effect of single-port video-assisted thoracoscopic surgery（VATS）for lung cancer radical resection on the immune function of patients. Methods A retrospective analysis was made on the clinical data of 42 patients with lung cancer radical resection by single-port VATS and 45 patients by thoracotomy in the department of thoracic surgery from January 2014 to November 2014. The data of immune function including T，Th，Ts，NK，CIK，CIKN cells and TH/TS were compared between the two groups in the preoperative and postoperative day 3. Results No operative related mortality. There were no statistical differences in the operation time， intraoperative blood loss，and chest closed drainage tube drawing time between the two groups（P > 0.05）. In patients with lung cancer radical resection by single-port VATS， postoperative pain， out-of-bed activity and immune indexes were better than patients by thoracotomy（P < 0.05）. Conclusion The single-port VATS for patients can decrease the impact by lung cancer radical resection.
［Abstract］Objective The study was aimed to analyze the effect of single-port video-assisted thoracoscopic surgery（VATS）for lung cancer radical resection on the immune function of patients. Methods A retrospective analysis was made on the clinical data of 42 patients with lung cancer radical resection by single-port VATS and 45 patients by thoracotomy in the department of thoracic surgery from January 2014 to November 2014. The data of immune function including T，Th，Ts，NK，CIK，CIKN cells and TH/TS were compared between the two groups in the preoperative and postoperative day 3. Results No operative related mortality. There were no statistical differences in the operation time， intraoperative blood loss，and chest closed drainage tube drawing time between the two groups（P > 0.05）. In patients with lung cancer radical resection by single-port VATS， postoperative pain， out-of-bed activity and immune indexes were better than patients by thoracotomy（P < 0.05）. Conclusion The single-port VATS for patients can decrease the impact by lung cancer radical resection.
2016, 37(03)
Abstract:
［Abstract］Objective To investigate the clinical efficacy of percutaneous transhepatic gallbladder drainage （PTGD）combined with laparoscopic cholecystectomy（LC）in the treatment of acute obstruction suppurative cholecystitis in high-risk and elderly patients. Methods Retrospective analysis was made on the clinical data of 56 patients with acute severe cholecystitis who underwent PTGD and two-stage LC from Sep 2013 to Sep 2014. Results PTGD was performed successfully for all patients. There were no biliary fistula，bleeding and other complications after PTGD. The symptoms of abdominal pain were relieved at 24h after operation. And all cases were performed LC after 30 days of PTGD， in which 3 patients were transferred to open cholecystectomy（5.3%）. No patient had severe complication or died after LC. Conclusion With the low conversion to open surgery and less complications， PTGD combined two-stage LC is simple， effective and safe for the treatment of acute obstruction suppurative cholecystitis in high-risk and elderly patients.
［Abstract］Objective To investigate the clinical efficacy of percutaneous transhepatic gallbladder drainage （PTGD）combined with laparoscopic cholecystectomy（LC）in the treatment of acute obstruction suppurative cholecystitis in high-risk and elderly patients. Methods Retrospective analysis was made on the clinical data of 56 patients with acute severe cholecystitis who underwent PTGD and two-stage LC from Sep 2013 to Sep 2014. Results PTGD was performed successfully for all patients. There were no biliary fistula，bleeding and other complications after PTGD. The symptoms of abdominal pain were relieved at 24h after operation. And all cases were performed LC after 30 days of PTGD， in which 3 patients were transferred to open cholecystectomy（5.3%）. No patient had severe complication or died after LC. Conclusion With the low conversion to open surgery and less complications， PTGD combined two-stage LC is simple， effective and safe for the treatment of acute obstruction suppurative cholecystitis in high-risk and elderly patients.
2016, 37(03)
Abstract:
［Abstract］Objective To investigate the correlation of oral lichen planus（OLP）with Helicobacter pylori （HP）. Methods The combination of polymerase chain reaction（PCR） technology and 14C urea breath test were used to detect the presence of HP in 65 confirmed OLP patient's mouth and stomach. Results HP infection rate in mouth was significantly higher than that in stomach of OLP patients，and the difference was significant（χ2 = 8.795，P < 0.05）. For OLP patients with a history of chronic gastropathy，HP positive results in stomach and mouth were 23 cases and 27 cases， respectively. For OLP patients without a history of chronic gastropathy， HP positive results in stomach and mouth were 11 cases and 20 cases， respectively. Conclusion OLP may have a correlation with HP infection.
［Abstract］Objective To investigate the correlation of oral lichen planus（OLP）with Helicobacter pylori （HP）. Methods The combination of polymerase chain reaction（PCR） technology and 14C urea breath test were used to detect the presence of HP in 65 confirmed OLP patient's mouth and stomach. Results HP infection rate in mouth was significantly higher than that in stomach of OLP patients，and the difference was significant（χ2 = 8.795，P < 0.05）. For OLP patients with a history of chronic gastropathy，HP positive results in stomach and mouth were 23 cases and 27 cases， respectively. For OLP patients without a history of chronic gastropathy， HP positive results in stomach and mouth were 11 cases and 20 cases， respectively. Conclusion OLP may have a correlation with HP infection.
2016, 37(03)
Abstract:
［Abstract］Objective To investigate the therapeutic effect of Algoplaque film on phlebitis caused by intravenous infusion of KLT. Methods 52 patients with phlebitis were randomly divided into two groups. The experimental group was given Algoplaque film as therapy of phlebitis and the control group was treated with 50% magnesium sulfate. Results The total effective rate of experimental group was 96%, which had significant difference compared with the control group of 77%（P < 0.05）. Conclusion Experimental group get better therapeutic effect than control group. The therapy of Algoplaque film on phlebitis caused by KLT is worthy to be popularized.
［Abstract］Objective To investigate the therapeutic effect of Algoplaque film on phlebitis caused by intravenous infusion of KLT. Methods 52 patients with phlebitis were randomly divided into two groups. The experimental group was given Algoplaque film as therapy of phlebitis and the control group was treated with 50% magnesium sulfate. Results The total effective rate of experimental group was 96%, which had significant difference compared with the control group of 77%（P < 0.05）. Conclusion Experimental group get better therapeutic effect than control group. The therapy of Algoplaque film on phlebitis caused by KLT is worthy to be popularized.
2016, 37(03)
Abstract:
［Abstract］Objective To analyze the effect of early intervention in neurodevelopment on the motor and cognitive development of high risk premature infants. Methods 82 children with high risk premature infants in weinan central hospital from May 2013 to February 2015 were selected and randomly divided into control group （41 cases） and observation group （41 cases）. Patients in the control group were given a conventional treatment and care，J62 while patients in the observation group were given early intervention in neurodevelopment. The motor and cognitive development of patients in the two groups was compared. Results PDI scores of patients in the observation group were（85.74±16.80），（94.56±15.68），（86.52±16.34）and（87.21±16.85）in 3，6，9 and 12 months， which was significantly higher than those in the control group［（60.52±13.57），（72.15±15.38）， （62.68±15.94）and（63.92±13.80），P < 0.05］，respectively. MDI scores of patients in the observation group were（83.52±12.58），（93.48±13.57）and（85.91±16.27）in 3，6 and 9 months，which was significantly higher than those in the control group［（61.35±13.57），（71.26±15.34）and（61.45±15.28）， P < 0.05］， respectively. The incidence of cerebral palsy in the observation group （2.4%） was lower than that in the control group （4.9%）， but there was no significant difference（χ2 = 0.346，P = 0.556）. Conclusion In the treatment of high risk premature infants， early intervention in neurodevelopment can improve the motor and cognitive development， which is worthy of application.
［Abstract］Objective To analyze the effect of early intervention in neurodevelopment on the motor and cognitive development of high risk premature infants. Methods 82 children with high risk premature infants in weinan central hospital from May 2013 to February 2015 were selected and randomly divided into control group （41 cases） and observation group （41 cases）. Patients in the control group were given a conventional treatment and care，J62 while patients in the observation group were given early intervention in neurodevelopment. The motor and cognitive development of patients in the two groups was compared. Results PDI scores of patients in the observation group were（85.74±16.80），（94.56±15.68），（86.52±16.34）and（87.21±16.85）in 3，6，9 and 12 months， which was significantly higher than those in the control group［（60.52±13.57），（72.15±15.38）， （62.68±15.94）and（63.92±13.80），P < 0.05］，respectively. MDI scores of patients in the observation group were（83.52±12.58），（93.48±13.57）and（85.91±16.27）in 3，6 and 9 months，which was significantly higher than those in the control group［（61.35±13.57），（71.26±15.34）and（61.45±15.28）， P < 0.05］， respectively. The incidence of cerebral palsy in the observation group （2.4%） was lower than that in the control group （4.9%）， but there was no significant difference（χ2 = 0.346，P = 0.556）. Conclusion In the treatment of high risk premature infants， early intervention in neurodevelopment can improve the motor and cognitive development， which is worthy of application.
2016, 37(03)
Abstract:
［Abstract］Objective The study was aimed to investigate the prevention and treatment of intraoperative hypotension with combined spinal-epidural anesthesia for cesarean section. Methods Eighty-two patients were randomly selected who underwent cesarean section under combined spinal and epidural anesthesia from September 2012 to September 2013 in our hospital. Patients were divided into research and control groups. The general preparation was given to patients in the control group， and a standard and systematic of preoperative and intraoperative intervention were implemented for patients in the research group. Their clinical data were analyzed retrospectively. Results The percentage of intraoperative hypotension happened in the research group was 12.20%，which was significantly lower than that in the control group（21.95%），with a statistical significant difference（P < 0.05）. The patient's satisfaction was 97.56% in the research group，which was superior to 87.80% in the control group（P < 0.05）. Conclusion The necessary preoperative and intraoperative intervention could effectively reduce the occurrence of hypotension during combined spinal-epidural anesthesia for cesarean section， relieve the sufferings of the patient，and effectively promote the recovery after surgery. It is worthy to be widely applied.
［Abstract］Objective The study was aimed to investigate the prevention and treatment of intraoperative hypotension with combined spinal-epidural anesthesia for cesarean section. Methods Eighty-two patients were randomly selected who underwent cesarean section under combined spinal and epidural anesthesia from September 2012 to September 2013 in our hospital. Patients were divided into research and control groups. The general preparation was given to patients in the control group， and a standard and systematic of preoperative and intraoperative intervention were implemented for patients in the research group. Their clinical data were analyzed retrospectively. Results The percentage of intraoperative hypotension happened in the research group was 12.20%，which was significantly lower than that in the control group（21.95%），with a statistical significant difference（P < 0.05）. The patient's satisfaction was 97.56% in the research group，which was superior to 87.80% in the control group（P < 0.05）. Conclusion The necessary preoperative and intraoperative intervention could effectively reduce the occurrence of hypotension during combined spinal-epidural anesthesia for cesarean section， relieve the sufferings of the patient，and effectively promote the recovery after surgery. It is worthy to be widely applied.
2016, 37(03)
Abstract:
［Abstract］Objective To investigate the application value of ultrasound elastography and two-dimensional ultrasound in benign and malignant lesions of liver. Methods 84 patients with liver disease were selected as research objects who underwent preoperative ultrasound elastography and conventional ultrasonography from June 2012 to June 2014. A comparison was made between the results of imaging diagnosis and surgical pathology. Results There were 38 cases （68 lesions） of benign malignant liver lesions and 46 cases of malignant tumors（82 lesions）. For benign and malignant lesions，the diagnostic accuracy，sensitivity and specificity of dimensional ultrasound diagnosis were 76.47% and 82.93%，70.25% and 68.23%，and 62.45% and 72.32%，respectively. However， the diagnostic accuracy，sensitivity and specificity of ultrasound elastography were 91.17% and 95.12%， 93.65% and 90.25%，and 80.25% and 88.92%， respectively. There were the statistically significant differences between the two methods（P < 0.05）. Compared with benign lesions，malignant lesions showed higher grading using ultrasound elastography，with a statistically significant difference（P < 0.05）. Conclusion Ultrasound elastography technique provides a new method to diagnose the liver diseases，which can improve the accuracy and sensitivity of the diagnosis of benign and malignant lesions.
［Abstract］Objective To investigate the application value of ultrasound elastography and two-dimensional ultrasound in benign and malignant lesions of liver. Methods 84 patients with liver disease were selected as research objects who underwent preoperative ultrasound elastography and conventional ultrasonography from June 2012 to June 2014. A comparison was made between the results of imaging diagnosis and surgical pathology. Results There were 38 cases （68 lesions） of benign malignant liver lesions and 46 cases of malignant tumors（82 lesions）. For benign and malignant lesions，the diagnostic accuracy，sensitivity and specificity of dimensional ultrasound diagnosis were 76.47% and 82.93%，70.25% and 68.23%，and 62.45% and 72.32%，respectively. However， the diagnostic accuracy，sensitivity and specificity of ultrasound elastography were 91.17% and 95.12%， 93.65% and 90.25%，and 80.25% and 88.92%， respectively. There were the statistically significant differences between the two methods（P < 0.05）. Compared with benign lesions，malignant lesions showed higher grading using ultrasound elastography，with a statistically significant difference（P < 0.05）. Conclusion Ultrasound elastography technique provides a new method to diagnose the liver diseases，which can improve the accuracy and sensitivity of the diagnosis of benign and malignant lesions.
2016, 37(03)
Abstract:
［Abstract］Objective To compare the efficacy between recombinant tissue plasminogen activator intravenous thrombolytic therapy and conventional antiplatelet therapy for acute cerebral infarction. Methods 120 patients with acute cerebral infarction in Tianjin Xiqing hospital from Jan 2013 to Feb 2015 were randomly divided into the control group （conventional antiplatelet therapy）and the observation group（recombinant tissue plasminogen activator intravenous thrombolytic therapy）according to the random number method，60 patients per group. The clinical efficacy， the improvement of nerve function defect and daily living ability on pre- and post-treatment in the two groups were compared according to the NIHSS score and Barthel index（BI）. Results Compared with the control group， the cure rate and total effective rate were significantly increased （P < 0.05），NIHSS scores were obviously reduced on post-treatment（P < 0.05），and BI scores were significantly increased on post-treatment in the observation group（P < 0.05）. Conclusion The recombinant tissue plasminogen activator intravenous thrombolytic therapy can significantly increase the clinical efficacy for acute cerebral infarction and improve the nerve function defect and daily living ability， which is worthy of clinical promotion.
［Abstract］Objective To compare the efficacy between recombinant tissue plasminogen activator intravenous thrombolytic therapy and conventional antiplatelet therapy for acute cerebral infarction. Methods 120 patients with acute cerebral infarction in Tianjin Xiqing hospital from Jan 2013 to Feb 2015 were randomly divided into the control group （conventional antiplatelet therapy）and the observation group（recombinant tissue plasminogen activator intravenous thrombolytic therapy）according to the random number method，60 patients per group. The clinical efficacy， the improvement of nerve function defect and daily living ability on pre- and post-treatment in the two groups were compared according to the NIHSS score and Barthel index（BI）. Results Compared with the control group， the cure rate and total effective rate were significantly increased （P < 0.05），NIHSS scores were obviously reduced on post-treatment（P < 0.05），and BI scores were significantly increased on post-treatment in the observation group（P < 0.05）. Conclusion The recombinant tissue plasminogen activator intravenous thrombolytic therapy can significantly increase the clinical efficacy for acute cerebral infarction and improve the nerve function defect and daily living ability， which is worthy of clinical promotion.
2016, 37(03)
Abstract:
［Abstract］Objective To explore the changes in serum levels of CRP， IL-6 and TNF-α in patients with endometriosis（EMS），and evaluate the clinical efficacy of laparoscopic operation combined with dexamethasone cavity flushing in endometriosis. Methods From November 2013 to December 2014，80 patients underwent endometriosis surgery in our hospital including simple laparoscopic surgery in 20 patients（L group），laparoscopic surgery combined with dexamethasone cavity flushing in 20 patients （LD group）， simple open operation in 20 patients（O group），and open operation combined with dexamethasone cavity flushing in 20 patients（OD group）. The serum levels of CRP， IL-6 and TNF-α in all patients were tested before surgery and on the first and third postoperative days. Results Postoperative serum inflammatory factor levels of all groups were increased significantly. The postoperative serum levels of CRP， IL-6 and TNF-α in LD group were increased mildest with the minimum values， and the values could recover to the preoperative level closely on the third postoperative days（P < 0.05）. Conclusion Laparoscopic operation combined with dexamethasone cavity flushing has definite clinical effect on endometriosis and less adverse reactions.
［Abstract］Objective To explore the changes in serum levels of CRP， IL-6 and TNF-α in patients with endometriosis（EMS），and evaluate the clinical efficacy of laparoscopic operation combined with dexamethasone cavity flushing in endometriosis. Methods From November 2013 to December 2014，80 patients underwent endometriosis surgery in our hospital including simple laparoscopic surgery in 20 patients（L group），laparoscopic surgery combined with dexamethasone cavity flushing in 20 patients （LD group）， simple open operation in 20 patients（O group），and open operation combined with dexamethasone cavity flushing in 20 patients（OD group）. The serum levels of CRP， IL-6 and TNF-α in all patients were tested before surgery and on the first and third postoperative days. Results Postoperative serum inflammatory factor levels of all groups were increased significantly. The postoperative serum levels of CRP， IL-6 and TNF-α in LD group were increased mildest with the minimum values， and the values could recover to the preoperative level closely on the third postoperative days（P < 0.05）. Conclusion Laparoscopic operation combined with dexamethasone cavity flushing has definite clinical effect on endometriosis and less adverse reactions.
2016, 37(03)
Abstract:
［Abstract］Objectives The goal of this study was to analyze the clinical significance of platelet distribution width（PDW）and mean platelet volume（MPV）levels in the prognosis of chronic obstructive pulmonary disease （COPD）combined with pulmonary artery hypertension（PAH）. Methods In a retrospective study， we identified 498 patients with COPD from January 2012 to December 2013 in the second people's hospital of Qujing， 216 of them combined with PAH. We recorded the clinical data， results of blood gas analysis and echocardiography and measured the levels of PDW，MPV and NT-proBNP after admission. The results were statistically analyzed by the statistical package for social sciences（SPSS version 18.0） and software SAS JMP 9.0. Results The levels of PDW［（17.9±1.9%）vs（16.9±2.3）%，P＝0.008］， MPV ［（13.9±1.5）fL vs（12.4±2.1）fL，P＝0.002］and NT-proBNP［（1521.4±925.7）pg/mL vs（691.9±364.5）pg/mL，P＜0.001］in COPD with PAH group were significantly higher than those in COPD without PAH group. At follow up of 12 months， patients with readmission or death had higher values of PDW［（19.1±1.8）% vs（17.6±1.4）%，P＜0.001］and MPV ［（14.5±1.5）fL vs（13.7±1.4）fL，P＝0.002］. Kaplan-Meier survival analysis showed that the rate of readmission and death after 12 months was significantly higher in patients with higher values of PDW（P＝0.018） and MPV （P＝0.016）. Conclusions The detection of PDW and MPV has great signification for the prediction of long-term outcomes in COPD combined with PAH.
［Abstract］Objectives The goal of this study was to analyze the clinical significance of platelet distribution width（PDW）and mean platelet volume（MPV）levels in the prognosis of chronic obstructive pulmonary disease （COPD）combined with pulmonary artery hypertension（PAH）. Methods In a retrospective study， we identified 498 patients with COPD from January 2012 to December 2013 in the second people's hospital of Qujing， 216 of them combined with PAH. We recorded the clinical data， results of blood gas analysis and echocardiography and measured the levels of PDW，MPV and NT-proBNP after admission. The results were statistically analyzed by the statistical package for social sciences（SPSS version 18.0） and software SAS JMP 9.0. Results The levels of PDW［（17.9±1.9%）vs（16.9±2.3）%，P＝0.008］， MPV ［（13.9±1.5）fL vs（12.4±2.1）fL，P＝0.002］and NT-proBNP［（1521.4±925.7）pg/mL vs（691.9±364.5）pg/mL，P＜0.001］in COPD with PAH group were significantly higher than those in COPD without PAH group. At follow up of 12 months， patients with readmission or death had higher values of PDW［（19.1±1.8）% vs（17.6±1.4）%，P＜0.001］and MPV ［（14.5±1.5）fL vs（13.7±1.4）fL，P＝0.002］. Kaplan-Meier survival analysis showed that the rate of readmission and death after 12 months was significantly higher in patients with higher values of PDW（P＝0.018） and MPV （P＝0.016）. Conclusions The detection of PDW and MPV has great signification for the prediction of long-term outcomes in COPD combined with PAH.
2016, 37(03)
Abstract:
［Abstract］Objective To investigate the effect of whole course nursing in emergency treatment of patients with ST segment elevation myocardial infarction（STEMI）. Methods 140 patients with STEMI were randomly and equally divided into observation group and control group. Patients in the control group received routine nursing method， and the observation group was given the whole process optimization nursing. The rescue success rate，first aid time （including the assessment time，the establishment time of the venous path， ECG time，total first aid time）and nursing satisfaction between the two groups of patients were compared. Results Compared with the control group， the success rate was significantly improved， the first aid time was significantly shortened， and the patients' satisfaction was higher in the observation group. The differences were statistically significant（P < 0.05）. Conclusion The whole process of optimizing nursing can shorten the emergency time，increase the success rate， improve the patients' satisfaction，which is worthy of clinical promotion.
［Abstract］Objective To investigate the effect of whole course nursing in emergency treatment of patients with ST segment elevation myocardial infarction（STEMI）. Methods 140 patients with STEMI were randomly and equally divided into observation group and control group. Patients in the control group received routine nursing method， and the observation group was given the whole process optimization nursing. The rescue success rate，first aid time （including the assessment time，the establishment time of the venous path， ECG time，total first aid time）and nursing satisfaction between the two groups of patients were compared. Results Compared with the control group， the success rate was significantly improved， the first aid time was significantly shortened， and the patients' satisfaction was higher in the observation group. The differences were statistically significant（P < 0.05）. Conclusion The whole process of optimizing nursing can shorten the emergency time，increase the success rate， improve the patients' satisfaction，which is worthy of clinical promotion.
2016, 37(03)
Abstract:
［Abstract］Objective To analyze the relationship between premature rupture of membranes and Candida and aerobic bacteria infection， and investigate the influence of Candida and aerobic bacteria infection on pregnancy outcomes. Methods 200 pregnant women in Liuzhou maternal and child health-care hospital from Jan 2012 to Jan 2014 were enrolled in this study. They were divided into three groups，70 patients in preterm premature rupture of membrane group（PPROM group），70 patients in term premature rupture of membrane group（TPROM group） and 60 cases in normal pregnancy group. The cervical secretions were collected to detect Candida and aerobic bacteria infection. Effect of Candida and aerobic bacteria infection on pregnancy outcomes was analyzed. Results Pathogenic bacteria infection rates of PPROM group and TPROM group were higher than that of normal pregnancy group， pathogenic bacteria infection rates of PPROM group was higher than that of pathogenic bacteria infection rates of TPROM group，and the difference were all significant（P < 0.05）. The patients with Candida and aerobic bacteria infection were enrolled as infected group， and patients without Candida and aerobic bacteria infection were enrolled as uninfected group. The rates of premature birth， cesarean section， neonatal infection，low birth weight and perinatal diseases in infected group were significantly higher than those in uninfected group（P < 0.05）. Pregnancy outcomes were compared between patients with Candida infection and aerobic bacteria infection. As the results， there were no significantly differences in the rates of premature birth， cesarean section， neonatal infection， fetal distress， perinatal diseases and perinatal death（P > 0.05）， but there was a significantly difference in the rate of low birth weight between patients with Candida infection and aerobic bacteria infection（P < 0.05）. Conclusion Candida and aerobic bacteria infection are closely related to poor pregnancy outcomes of premature rupture of membranes. Examination of pathogenic microorganism and effective antibiotic treatment can reduce the rate of premature rupture of membrane and improve maternal and neonatal outcomes.
［Abstract］Objective To analyze the relationship between premature rupture of membranes and Candida and aerobic bacteria infection， and investigate the influence of Candida and aerobic bacteria infection on pregnancy outcomes. Methods 200 pregnant women in Liuzhou maternal and child health-care hospital from Jan 2012 to Jan 2014 were enrolled in this study. They were divided into three groups，70 patients in preterm premature rupture of membrane group（PPROM group），70 patients in term premature rupture of membrane group（TPROM group） and 60 cases in normal pregnancy group. The cervical secretions were collected to detect Candida and aerobic bacteria infection. Effect of Candida and aerobic bacteria infection on pregnancy outcomes was analyzed. Results Pathogenic bacteria infection rates of PPROM group and TPROM group were higher than that of normal pregnancy group， pathogenic bacteria infection rates of PPROM group was higher than that of pathogenic bacteria infection rates of TPROM group，and the difference were all significant（P < 0.05）. The patients with Candida and aerobic bacteria infection were enrolled as infected group， and patients without Candida and aerobic bacteria infection were enrolled as uninfected group. The rates of premature birth， cesarean section， neonatal infection，low birth weight and perinatal diseases in infected group were significantly higher than those in uninfected group（P < 0.05）. Pregnancy outcomes were compared between patients with Candida infection and aerobic bacteria infection. As the results， there were no significantly differences in the rates of premature birth， cesarean section， neonatal infection， fetal distress， perinatal diseases and perinatal death（P > 0.05）， but there was a significantly difference in the rate of low birth weight between patients with Candida infection and aerobic bacteria infection（P < 0.05）. Conclusion Candida and aerobic bacteria infection are closely related to poor pregnancy outcomes of premature rupture of membranes. Examination of pathogenic microorganism and effective antibiotic treatment can reduce the rate of premature rupture of membrane and improve maternal and neonatal outcomes.
2016, 37(03)
Abstract:
［Abstract］Objective To evaluate the feasibility and safety of low atrial septum pacing， and investigate a simplified location methods for low atrial septum pacing. Methods Forty eight patients with pacemaker implantation standard，were randomly divided into two groups. Group A（n＝24）with low atrial septum pacing by active fixation electrode（LAS group），and group B（n＝24）with traditional right atrial appendage pacing by electrode passive fixation（RAA group）. The atrial electrode operating time， the success rate of implantation were recorded，and the pacing parameters were measured in first week，first months and third months after operation，and associated complications including electrode dislocation，pace pocket infection and blood，pericardial effusion was observed. Results There were 21 cases success in atrial electrode location operation and 3 cases of failure in LAS group， while all operations were completed successfully in RAA group. Atrial electrode operation time had significant difference between two groups. The operate time of LAS group was longer than that of RAA group（6.3±0.8 min vs 3.5±0.5 min，P <0.05）. Pacing parameters remained stable during operation and on the first week，first and third months post-operative， and no significant differences existed between the two groups. No complications occurred during 3 months follow up. Conclusion The new simplified design technique of J shape duo-curve guide wire could increase the success rate of low atrial septum pacing. Low atrial septum pacing through active fixation electrode is safe and feasible.
［Abstract］Objective To evaluate the feasibility and safety of low atrial septum pacing， and investigate a simplified location methods for low atrial septum pacing. Methods Forty eight patients with pacemaker implantation standard，were randomly divided into two groups. Group A（n＝24）with low atrial septum pacing by active fixation electrode（LAS group），and group B（n＝24）with traditional right atrial appendage pacing by electrode passive fixation（RAA group）. The atrial electrode operating time， the success rate of implantation were recorded，and the pacing parameters were measured in first week，first months and third months after operation，and associated complications including electrode dislocation，pace pocket infection and blood，pericardial effusion was observed. Results There were 21 cases success in atrial electrode location operation and 3 cases of failure in LAS group， while all operations were completed successfully in RAA group. Atrial electrode operation time had significant difference between two groups. The operate time of LAS group was longer than that of RAA group（6.3±0.8 min vs 3.5±0.5 min，P <0.05）. Pacing parameters remained stable during operation and on the first week，first and third months post-operative， and no significant differences existed between the two groups. No complications occurred during 3 months follow up. Conclusion The new simplified design technique of J shape duo-curve guide wire could increase the success rate of low atrial septum pacing. Low atrial septum pacing through active fixation electrode is safe and feasible.
2016, 37(03)
Abstract:
［Abstract］Objective The study was aimed to definite the medicinal value of Syzygium aromaticum in oral clinical medicine by determining its antibacterial effect on the growth of oral predominant bacteria in vitro. Methods Agar dilution method was used to determine the antimicrobial activity of S. aromaticum to 21 kinds of oral model strains and clinical isolates. The results were expressed by minimum inhibitory concentration（MIC）. Results S. aromaticum was effective to all the oral predominant bacteria. The MIC was 20 mg/ml for ten kinds of cariogenic bacteria. For eleven kinds of predominant bacteria which normally involved in pulp periapical disease and periodontitis，the MICs were between 2.5 mg/mL and 10 mg/ml. Conclusion S. aromaticum could inhibit the growth of normal oral predominant bacteria in vitro. It could be used in the treatment or prevention of oral bacterial diseases.
［Abstract］Objective The study was aimed to definite the medicinal value of Syzygium aromaticum in oral clinical medicine by determining its antibacterial effect on the growth of oral predominant bacteria in vitro. Methods Agar dilution method was used to determine the antimicrobial activity of S. aromaticum to 21 kinds of oral model strains and clinical isolates. The results were expressed by minimum inhibitory concentration（MIC）. Results S. aromaticum was effective to all the oral predominant bacteria. The MIC was 20 mg/ml for ten kinds of cariogenic bacteria. For eleven kinds of predominant bacteria which normally involved in pulp periapical disease and periodontitis，the MICs were between 2.5 mg/mL and 10 mg/ml. Conclusion S. aromaticum could inhibit the growth of normal oral predominant bacteria in vitro. It could be used in the treatment or prevention of oral bacterial diseases.
2016, 37(03)
Abstract:
［Abstract］Objective The study was aimed to determine a better way in transitional occlusal reconstruction by comparing the restorative effects of fixed temporary crown and bridge or splint removable partial dentures. Methods Fixed temporary crown and bridge and splint removable partial denture for dentition defect were used as transitional restorative ways of severe abrasion to restore 1/3 vertical distance of patients temporarily，recover occlusal relationship， and improve joint symptoms. The effects of the two ways were observed by follow-up. Results After wearing the two prosthesis， patients consciously feel comfortable without pain. The two ways could improve beauty of face and masticatory function and eliminate food impaction， but the fixed temporary crown and bridge was more effective in improving masticatory function than splint removable partial dentures. Conclusion Temporary crown and bridge repair was superior to splint fixed pad removable partial denture in improving masticatory function in patients.
［Abstract］Objective The study was aimed to determine a better way in transitional occlusal reconstruction by comparing the restorative effects of fixed temporary crown and bridge or splint removable partial dentures. Methods Fixed temporary crown and bridge and splint removable partial denture for dentition defect were used as transitional restorative ways of severe abrasion to restore 1/3 vertical distance of patients temporarily，recover occlusal relationship， and improve joint symptoms. The effects of the two ways were observed by follow-up. Results After wearing the two prosthesis， patients consciously feel comfortable without pain. The two ways could improve beauty of face and masticatory function and eliminate food impaction， but the fixed temporary crown and bridge was more effective in improving masticatory function than splint removable partial dentures. Conclusion Temporary crown and bridge repair was superior to splint fixed pad removable partial denture in improving masticatory function in patients.
2016, 37(03)
Abstract:
［Abstract］Objective To explore the application of Climen in difficult removal of intrauterine device （IUD） in postmenopausal women. Methods 50 cases who underwent the failure to take out IUD were given Climen， then the degree of cervical softening，comprehensive reaction degree and success rate were observed before and at different periods after oral Climen. Results After oral Climen for three months， the degree of cervical softening and successful rate were significantly higher than those before treatment and after treatment for 1 month （P < 0.01）， while the response was significantly lower than that before treatment and after treatment for 1 month （P < 0.01）. Conclusion Climen is effective for postmenopausal women to removal IUD， and oral Climen for 3 months is especially recommended. It can reduce the occurrence of comprehensive reaction of patients and improve the success rate. It is worthy of clinical promotion and application.
［Abstract］Objective To explore the application of Climen in difficult removal of intrauterine device （IUD） in postmenopausal women. Methods 50 cases who underwent the failure to take out IUD were given Climen， then the degree of cervical softening，comprehensive reaction degree and success rate were observed before and at different periods after oral Climen. Results After oral Climen for three months， the degree of cervical softening and successful rate were significantly higher than those before treatment and after treatment for 1 month （P < 0.01）， while the response was significantly lower than that before treatment and after treatment for 1 month （P < 0.01）. Conclusion Climen is effective for postmenopausal women to removal IUD， and oral Climen for 3 months is especially recommended. It can reduce the occurrence of comprehensive reaction of patients and improve the success rate. It is worthy of clinical promotion and application.
2016, 37(03)
Abstract:
［Abstract］Objective To investigate the clinical efficacy of 23-gauge transconjunctival sutureless vitrectomy （23G TSV） system in the treatment of vitreous hemorrhage. Methods 38 patients （43 eyes） underwent 23-gauge vitrectomy in our hospital from May 2013 to June 2015， and their clinical data were retrospectively analyzed. All patients were followed up 2 to 14 months after operation. We observed the best corrected visual acuity （BCVA），intraocular pressure（IOP）and postoperative complications. Results All cases were successfully performed and the postoperative BCVA had been improved. There were significant differences in BCVA between postoperative and preoperative（P < 0.05）. Few postoperative complications were observed， including cataract in 6 eyes， high IOP in 1 eye and low IOP in 3 eyes. However， all complications disappeared after a basic treatment. Conclusion 23G TSV system is an effective and safe surgical technique in the management of vitreous hemorrhage， which can improve the patients' visual and avoid the loss of visual function. It is worthy of clinical promotion.
［Abstract］Objective To investigate the clinical efficacy of 23-gauge transconjunctival sutureless vitrectomy （23G TSV） system in the treatment of vitreous hemorrhage. Methods 38 patients （43 eyes） underwent 23-gauge vitrectomy in our hospital from May 2013 to June 2015， and their clinical data were retrospectively analyzed. All patients were followed up 2 to 14 months after operation. We observed the best corrected visual acuity （BCVA），intraocular pressure（IOP）and postoperative complications. Results All cases were successfully performed and the postoperative BCVA had been improved. There were significant differences in BCVA between postoperative and preoperative（P < 0.05）. Few postoperative complications were observed， including cataract in 6 eyes， high IOP in 1 eye and low IOP in 3 eyes. However， all complications disappeared after a basic treatment. Conclusion 23G TSV system is an effective and safe surgical technique in the management of vitreous hemorrhage， which can improve the patients' visual and avoid the loss of visual function. It is worthy of clinical promotion.
2016, 37(03)
Abstract:
［Abstract］Objective To investigate correlation between level of serum uric acid（SUA）and brachial ankle pulse wave（baPWV）in essential hypertension（EH）with ischemic stroke（IS）patients．Methods Forty-three patients with EH with IS，fifty-seven patients with EH and 49 normal subjects were selected randomly . All subjects were measured SUA and baPWV．we evaluated the association of SUA and baPWV in EH with IS patients．Results （1）The mean levels of Systolic blood pressure （SBP），diastolic blood pressure（DBP），Pulse pressure（PP）， the mean levels of SUA and baPWV were significantly higher in EH patients and EH with IS patients than control Patients ；the mean levels of SUA and baPWV in EH with IS patients were higher than EH patients（P＜0.05）.（2）After adjusting the factors of age，SBP，DBP，PP， triglyceride（TG）were still positively correlated（r = 0.451，P＜0.001） by partial correlation analysis.The multiple linear regression analysis showed that SUA and PP were the predictors of baPWV. Conclusion SUA is positively correlated with baPWV in patients with essential hypertension，and it can be used as an independent predictor. SUA is also the risk for patients with hypertension and stroke.
［Abstract］Objective To investigate correlation between level of serum uric acid（SUA）and brachial ankle pulse wave（baPWV）in essential hypertension（EH）with ischemic stroke（IS）patients．Methods Forty-three patients with EH with IS，fifty-seven patients with EH and 49 normal subjects were selected randomly . All subjects were measured SUA and baPWV．we evaluated the association of SUA and baPWV in EH with IS patients．Results （1）The mean levels of Systolic blood pressure （SBP），diastolic blood pressure（DBP），Pulse pressure（PP）， the mean levels of SUA and baPWV were significantly higher in EH patients and EH with IS patients than control Patients ；the mean levels of SUA and baPWV in EH with IS patients were higher than EH patients（P＜0.05）.（2）After adjusting the factors of age，SBP，DBP，PP， triglyceride（TG）were still positively correlated（r = 0.451，P＜0.001） by partial correlation analysis.The multiple linear regression analysis showed that SUA and PP were the predictors of baPWV. Conclusion SUA is positively correlated with baPWV in patients with essential hypertension，and it can be used as an independent predictor. SUA is also the risk for patients with hypertension and stroke.
2016, 37(03)
Abstract:
［Abstract］Objective To understand HIV/AIDS knowledge，attitude and behavior among medical students from different medical universities in Yunnan，and provide a scientific basis for a better health education strategy. Methods Using stratified random cluster sampling method， 600 （300 from each university）freshmen and sophomores from two universities（Kunming Medical University and Dali University）were selected and surveyed by anonymous questionnaire. Results There were statistically significant differences on HIV/AIDS knowledge，attitude toward AIDS patients and HIV infected populations，awareness and attitude on protective sexual behaviors between students from the two universities，with better situation of medical students from Kunming Medical University. Conclusion Medical students from Dali University have insufficient knowledge on HIV/AIDS，inadequate awareness on protective sexual behaviors， which should be improved by targeted health education on HIV/AIDS.
［Abstract］Objective To understand HIV/AIDS knowledge，attitude and behavior among medical students from different medical universities in Yunnan，and provide a scientific basis for a better health education strategy. Methods Using stratified random cluster sampling method， 600 （300 from each university）freshmen and sophomores from two universities（Kunming Medical University and Dali University）were selected and surveyed by anonymous questionnaire. Results There were statistically significant differences on HIV/AIDS knowledge，attitude toward AIDS patients and HIV infected populations，awareness and attitude on protective sexual behaviors between students from the two universities，with better situation of medical students from Kunming Medical University. Conclusion Medical students from Dali University have insufficient knowledge on HIV/AIDS，inadequate awareness on protective sexual behaviors， which should be improved by targeted health education on HIV/AIDS.
2016, 37(03)
Abstract:
［Abstract］In recent years，the next-generation sequencing （NGS）technology has gradually replaced the first generation with the advantages of fast， higher precision and low cost. The continuous innovation of gene sequencing technology principle and the new breakthroughs of engineering materials made high throughput gene sequencing technology meet the needs of individual diagnosis and treatment. At present， 454 sequencing technology， Solexa and SOLID sequencing technology are considered to be the representatives of the more mature NGS technology. This paper will introduce the basic principle， laboratory protocol， coefficient of performance and clinical application of NGS technology.
［Abstract］In recent years，the next-generation sequencing （NGS）technology has gradually replaced the first generation with the advantages of fast， higher precision and low cost. The continuous innovation of gene sequencing technology principle and the new breakthroughs of engineering materials made high throughput gene sequencing technology meet the needs of individual diagnosis and treatment. At present， 454 sequencing technology， Solexa and SOLID sequencing technology are considered to be the representatives of the more mature NGS technology. This paper will introduce the basic principle， laboratory protocol， coefficient of performance and clinical application of NGS technology.
2016, 37(03)
Abstract:
［Abstract］Objective To investigate the influence of comprehensive nursing intervention on clinical efficacy and complications of patients with nephrotic syndrome. Methods 74 patients with nephrotic syndrome in affiliated hospital of Panzhihua University from February 2012 to June 2015 were equally divided into observation group and control group，37 cases in each group. The control group received routine nursing intervention， while the observation group was given comprehensive nursing intervention， including psychological care， life care， medication guidance，prevention of complications and nursing discharge guidance. Clinical efficacy and complications were observed and compared between the two groups. Results The remission rate of the observation group was 94.6%， which was significantly higher than that of the control group（75.7），with a statistically significant difference（P < 0.05）. The complication rate of observation group was 8.1%， which was significantly lower than that of the control group（27.0%）， with a statistically significant difference（P < 0.05）. Conclusion Comprehensive nursing intervention can increase the clinical efficacy and reduce the incidence of complications to improve the prognosis of patients. It is worth promoting in clinic.
［Abstract］Objective To investigate the influence of comprehensive nursing intervention on clinical efficacy and complications of patients with nephrotic syndrome. Methods 74 patients with nephrotic syndrome in affiliated hospital of Panzhihua University from February 2012 to June 2015 were equally divided into observation group and control group，37 cases in each group. The control group received routine nursing intervention， while the observation group was given comprehensive nursing intervention， including psychological care， life care， medication guidance，prevention of complications and nursing discharge guidance. Clinical efficacy and complications were observed and compared between the two groups. Results The remission rate of the observation group was 94.6%， which was significantly higher than that of the control group（75.7），with a statistically significant difference（P < 0.05）. The complication rate of observation group was 8.1%， which was significantly lower than that of the control group（27.0%）， with a statistically significant difference（P < 0.05）. Conclusion Comprehensive nursing intervention can increase the clinical efficacy and reduce the incidence of complications to improve the prognosis of patients. It is worth promoting in clinic.
2016, 37(03)
Abstract:
［Abstract］Objective The study was aimed to compare the main points and function of clinical nursing between laparoscopic operation and laparotomy for cervical cancer. Methods Sixty-six patients with cervical cancer were randomly divided into two groups: laparoscopy group（LRH）and laparotomy group（ARH）. The experienced nurse practitioners with strong sense of responsibility were selected as duty nurses. Before operative， nursing planning was developed according to patients' condition in order to implement personalized nursing. The patients' situation was assessed after operation to improve the nursing plan. Then，a comparison were made in operating time， time for leaving bed，evacuation time， duration of pain，indwelling catheter time， body temperature and hospitalization days between the two groups. Results The duration of operation was （56±10.2） min and（54±11.3） min of LRH and ARH groups， respectively. There was no difference（P > 0.05）. Compared with the ARH group，the time for leaving bed，evacuation time，duration of pain，indwelling catheter time，body temperature and hospitalization days were significantly less than those of LRH group （P < 0.05）. Through effective nursing， no nursing complications occurred. Conclusion Radical surgery of cervical cancer is a large operation in gynecology. Laparoscopic surgery has advantages over laparotomy， including faster postoperative recovery and shorter operative time. Preoperative nursing is important guarantee of improving the success rate of the surgical resection，and the postoperative nursing plays a key role in the patients' recovery.
［Abstract］Objective The study was aimed to compare the main points and function of clinical nursing between laparoscopic operation and laparotomy for cervical cancer. Methods Sixty-six patients with cervical cancer were randomly divided into two groups: laparoscopy group（LRH）and laparotomy group（ARH）. The experienced nurse practitioners with strong sense of responsibility were selected as duty nurses. Before operative， nursing planning was developed according to patients' condition in order to implement personalized nursing. The patients' situation was assessed after operation to improve the nursing plan. Then，a comparison were made in operating time， time for leaving bed，evacuation time， duration of pain，indwelling catheter time， body temperature and hospitalization days between the two groups. Results The duration of operation was （56±10.2） min and（54±11.3） min of LRH and ARH groups， respectively. There was no difference（P > 0.05）. Compared with the ARH group，the time for leaving bed，evacuation time，duration of pain，indwelling catheter time，body temperature and hospitalization days were significantly less than those of LRH group （P < 0.05）. Through effective nursing， no nursing complications occurred. Conclusion Radical surgery of cervical cancer is a large operation in gynecology. Laparoscopic surgery has advantages over laparotomy， including faster postoperative recovery and shorter operative time. Preoperative nursing is important guarantee of improving the success rate of the surgical resection，and the postoperative nursing plays a key role in the patients' recovery.
