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RSS2016 Vol. 37, No. 10
2016, 37(10)
Abstract:
［Abstract］Multiple myeloma is a monoclonal plasma cell malignant proliferative disease，accounting for about 10% of malignant tumors of blood system. In recent years，due to the application of hematopoietic stem cell transplantation and the application of MDT by combining new drug such as thalidomide, lenalidomide and bortezomib,the average survival of MM patients was significantly longer than before. The treatment strategy should be individualized before treatment according to the patient's age，physical condition and complications .In this paper，the treatment strategy of multiple myeloma patients were reviewed.
［Abstract］Multiple myeloma is a monoclonal plasma cell malignant proliferative disease，accounting for about 10% of malignant tumors of blood system. In recent years，due to the application of hematopoietic stem cell transplantation and the application of MDT by combining new drug such as thalidomide, lenalidomide and bortezomib,the average survival of MM patients was significantly longer than before. The treatment strategy should be individualized before treatment according to the patient's age，physical condition and complications .In this paper，the treatment strategy of multiple myeloma patients were reviewed.
2016, 37(10)
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［Abstract］Objective To investigate the effect of different time of hypoxia reoxygenation （HR） on cell autophagy and cell viability．Methods According to the different reoxygenation time， H9C2 cells were divided into five groups: normal control group（group A）， hypoxia group （group B）， reoxygen 2h group （group C）， reoxygen 12h group（group D）and reoxygen 24h group （group E）. The expression of LC3 II/LC3 I in each groups was detected by Western Blot，and the activity of myocardial cells was detected by MTT. Results MTT results showed that the viability of the myocardial cells after hypoxia 2h/reoxygenation 2h was significantly lower than that in the normal control group（P＜0.05）. The ratio of LC3 II/LC3 I of the cells in group B （hypoxia group）， group C （reoxygen 2h group）， group D （reoxygen 12h group）， group E （reoxygen 24h group） was significantly higher than that of group A （normal control group） （P＜0.05）. Among them， the ratio of LC3 II/LC3 I of the cells in group C and D were significantly higher than that of group B（P＜0.05）， paticlularly the group D was the highest （P＜0.05） .The activity of myocardial cells in group D was the lowest among all groups. Conclusion Culturing in the three gas incubator can obviously decrease the viability of H9C2cells. Hypoxia can activate autophagy， and autophagy increases significantly after reoxygenation. Autophagy may be activated most obviously at 12 hours and decrease to the level of hypoxia group at 24 hours. Cells can be damaged by hypoxia， and further increase of cellular damage may occur after oxygen inhalation. At 12 hours，the cell viability is the lowest， and the cell viability may be improved at 24h.
［Abstract］Objective To investigate the effect of different time of hypoxia reoxygenation （HR） on cell autophagy and cell viability．Methods According to the different reoxygenation time， H9C2 cells were divided into five groups: normal control group（group A）， hypoxia group （group B）， reoxygen 2h group （group C）， reoxygen 12h group（group D）and reoxygen 24h group （group E）. The expression of LC3 II/LC3 I in each groups was detected by Western Blot，and the activity of myocardial cells was detected by MTT. Results MTT results showed that the viability of the myocardial cells after hypoxia 2h/reoxygenation 2h was significantly lower than that in the normal control group（P＜0.05）. The ratio of LC3 II/LC3 I of the cells in group B （hypoxia group）， group C （reoxygen 2h group）， group D （reoxygen 12h group）， group E （reoxygen 24h group） was significantly higher than that of group A （normal control group） （P＜0.05）. Among them， the ratio of LC3 II/LC3 I of the cells in group C and D were significantly higher than that of group B（P＜0.05）， paticlularly the group D was the highest （P＜0.05） .The activity of myocardial cells in group D was the lowest among all groups. Conclusion Culturing in the three gas incubator can obviously decrease the viability of H9C2cells. Hypoxia can activate autophagy， and autophagy increases significantly after reoxygenation. Autophagy may be activated most obviously at 12 hours and decrease to the level of hypoxia group at 24 hours. Cells can be damaged by hypoxia， and further increase of cellular damage may occur after oxygen inhalation. At 12 hours，the cell viability is the lowest， and the cell viability may be improved at 24h.
2016, 37(10)
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［Abstract］Objective To study the reactive oxygen level and the expression of anti-apoptotic protein Bcl-2 and pro-apoptotic protein Bax after treatment of DNA methyltransferase inhibitor 5-aza-2'-deoxycytidine （5-Aza-2'-dc） and paraquat in V79 cells．Methods Cultured V79 cells were divided into 5-Aza-2'-dc treatment group （group A）， paraquat treatment group（group B），5-Aza-2'-dc and paraquat treatment group （group C， V79 cells were pretreated with 5-Aza-2'-dc for 12h followed by exposure to paraquat for 12h）and control group （group D）. Reactive oxygen level in V79 cells was measured by DCFH-DA flow cytometry and expression of Bcl-2 and Bax was detected by Western blot．Results Reactive oxygen levels and expression levels of Bcl-2 and Bax in V79 cells were significantly different（P＜0.05） in 5-Aza-2'-dc and paraquat treatment group（group C），compared with 5-Aza-2'-dc treatment group （group A）， paraquat treatment group（group B） and control group（group D）. Expression levels of Bcl-2 and the ratio of Bcl-2 and Bax were lower while reactive oxygen levels and expression levels of Bax were higher in group C than in groups A， B and D. Conclusion 5-Aza-2'-dc regulates DNA methylation by the imbalancing the reactive oxygen metabolism and apoptosis， thus up-regulating the toxic effect of paraquat on V79 cells.
［Abstract］Objective To study the reactive oxygen level and the expression of anti-apoptotic protein Bcl-2 and pro-apoptotic protein Bax after treatment of DNA methyltransferase inhibitor 5-aza-2'-deoxycytidine （5-Aza-2'-dc） and paraquat in V79 cells．Methods Cultured V79 cells were divided into 5-Aza-2'-dc treatment group （group A）， paraquat treatment group（group B），5-Aza-2'-dc and paraquat treatment group （group C， V79 cells were pretreated with 5-Aza-2'-dc for 12h followed by exposure to paraquat for 12h）and control group （group D）. Reactive oxygen level in V79 cells was measured by DCFH-DA flow cytometry and expression of Bcl-2 and Bax was detected by Western blot．Results Reactive oxygen levels and expression levels of Bcl-2 and Bax in V79 cells were significantly different（P＜0.05） in 5-Aza-2'-dc and paraquat treatment group（group C），compared with 5-Aza-2'-dc treatment group （group A）， paraquat treatment group（group B） and control group（group D）. Expression levels of Bcl-2 and the ratio of Bcl-2 and Bax were lower while reactive oxygen levels and expression levels of Bax were higher in group C than in groups A， B and D. Conclusion 5-Aza-2'-dc regulates DNA methylation by the imbalancing the reactive oxygen metabolism and apoptosis， thus up-regulating the toxic effect of paraquat on V79 cells.
2016, 37(10)
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［Abstract］Objective To establish retroperitoneal orthotopic renal transplantation model in Diannan small-eared swine. Methods Ten pairs of Diannan small-eared swines， which with the same ABO blood type ，the negative of cross matching and complement-dependent cytotoxicity（CDC）cross reaction were performed with retroperitoneal orthotopic renal transplantation，end-to-end anastomosis method was implemented between the donor's and the receptor's renal artery，vein and ureter. Results One case appeared renal vein thrombosis，1 case appeared acute renal tubular necrosis，both resulted in the dysfunction of transplanted kidney，8 cases of postoperative graft function gradually returned to normal. No complications such as severe hemorrhage，infection， ureteral obstruction， urinary leakage ，intestinal obstruction happened，the graft survival rate was 80%．Conclusions Swine retroperitoneal orthotopic renal transplantation model is stable and reliable， with easier operational procedure， less complications and higher graft survival rate.
［Abstract］Objective To establish retroperitoneal orthotopic renal transplantation model in Diannan small-eared swine. Methods Ten pairs of Diannan small-eared swines， which with the same ABO blood type ，the negative of cross matching and complement-dependent cytotoxicity（CDC）cross reaction were performed with retroperitoneal orthotopic renal transplantation，end-to-end anastomosis method was implemented between the donor's and the receptor's renal artery，vein and ureter. Results One case appeared renal vein thrombosis，1 case appeared acute renal tubular necrosis，both resulted in the dysfunction of transplanted kidney，8 cases of postoperative graft function gradually returned to normal. No complications such as severe hemorrhage，infection， ureteral obstruction， urinary leakage ，intestinal obstruction happened，the graft survival rate was 80%．Conclusions Swine retroperitoneal orthotopic renal transplantation model is stable and reliable， with easier operational procedure， less complications and higher graft survival rate.
2016, 37(10)
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［Abstract］Objective To study the change of immune factor in COPD rats after intravenous injection of regulatory T cells. Methods Twenty-one SPF rats was divided into three groups at random， rat COPD model was built by smoking. We used magnetic bead isolation technic to separate CD4+CD25+ regulatory T cells. Regulatory T cells were cultured and injected into rats though rats' caudal vein according to different dose， 5×104/mL，5×105/mL，5×106/mL respectively. Flow cytometry was used to analyze cell factors. ELISA was used to analyze IL-6 and CRP．Results Adding JJ316 or IL-2 into medium benefited the proliferation of CD4+CD25+ regulatory T cells. On the 20 th day， regulatory T cells CD4+CD25+ proliferation stopped by adding JJ316 or IL-2 respectively. Regulatory T cells were cultured and injected into rats though rat caudal vein according to different dose. The levels of CRP and IL-6 were decreased when rats were injected by CD4+CD25+regulatory T cell after one week. Conclusions Injection of regulatory T cells is helpful to control inflammation progression of COPD， so the increase of regulatory T cells of patients with COPD may decrease inflammation progression of COPD.
［Abstract］Objective To study the change of immune factor in COPD rats after intravenous injection of regulatory T cells. Methods Twenty-one SPF rats was divided into three groups at random， rat COPD model was built by smoking. We used magnetic bead isolation technic to separate CD4+CD25+ regulatory T cells. Regulatory T cells were cultured and injected into rats though rats' caudal vein according to different dose， 5×104/mL，5×105/mL，5×106/mL respectively. Flow cytometry was used to analyze cell factors. ELISA was used to analyze IL-6 and CRP．Results Adding JJ316 or IL-2 into medium benefited the proliferation of CD4+CD25+ regulatory T cells. On the 20 th day， regulatory T cells CD4+CD25+ proliferation stopped by adding JJ316 or IL-2 respectively. Regulatory T cells were cultured and injected into rats though rat caudal vein according to different dose. The levels of CRP and IL-6 were decreased when rats were injected by CD4+CD25+regulatory T cell after one week. Conclusions Injection of regulatory T cells is helpful to control inflammation progression of COPD， so the increase of regulatory T cells of patients with COPD may decrease inflammation progression of COPD.
2016, 37(10)
Abstract:
［Abstract］Objective To observe liver pathological characteristics of the autoimmune hepatitis （AIH） C57BL/6 mice．Methods We established AIH C57BL/6 mice model with mixed adjuvants of allogenic liver antigens and complete freund's adjuvant by intraperitoneal injection. Then we determined and compared the body weight， alanine aminotransferase（ALT），aspartate aminotransferase（AST） between the normal control group （N group） and AIH model group（M group）. And we compared the pathological characteristics of liver，spleen， heart， lung， kidney between two groups. Results We successfully established AIH C57BL/6 mice model. Compared with N group， we found that the average weight increase of mice in M group was decreased，ALT and AST of mice in M group were boosted. In M group， liver of mice presented typical interface of hepatitis， lymphocytic infiltration，even severe hepatitis，and showed spotty necrosis， multi-acinar necrosis. Some showed early sign of liver fibrosis by aprearing fibrous tissue hyperplasia. M group mouse's spleens were enlarged significantly. The spleen had darker color， not neat， and not smooth. Meanwhile， results were analyzed with statistics to confirm whether there was a significant difference between two groups（P＜0.05）. About lungs， hearts and kidneys of mice in two groups，the pathological features were not found， and there was no statistic difference （P＞0.05）. Conclusions The liver pathological characteristics of AIH C57BL/6 mice are similar to AIH patients. The results provide the pathological basis for the experimental research of autoimmune hepatitis.
［Abstract］Objective To observe liver pathological characteristics of the autoimmune hepatitis （AIH） C57BL/6 mice．Methods We established AIH C57BL/6 mice model with mixed adjuvants of allogenic liver antigens and complete freund's adjuvant by intraperitoneal injection. Then we determined and compared the body weight， alanine aminotransferase（ALT），aspartate aminotransferase（AST） between the normal control group （N group） and AIH model group（M group）. And we compared the pathological characteristics of liver，spleen， heart， lung， kidney between two groups. Results We successfully established AIH C57BL/6 mice model. Compared with N group， we found that the average weight increase of mice in M group was decreased，ALT and AST of mice in M group were boosted. In M group， liver of mice presented typical interface of hepatitis， lymphocytic infiltration，even severe hepatitis，and showed spotty necrosis， multi-acinar necrosis. Some showed early sign of liver fibrosis by aprearing fibrous tissue hyperplasia. M group mouse's spleens were enlarged significantly. The spleen had darker color， not neat， and not smooth. Meanwhile， results were analyzed with statistics to confirm whether there was a significant difference between two groups（P＜0.05）. About lungs， hearts and kidneys of mice in two groups，the pathological features were not found， and there was no statistic difference （P＞0.05）. Conclusions The liver pathological characteristics of AIH C57BL/6 mice are similar to AIH patients. The results provide the pathological basis for the experimental research of autoimmune hepatitis.
2016, 37(10)
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［Abstract］Objective To establish a detection method to R292K and N294S mutation sites in H3N2 influenza A virus with TaqMan-MGB probes. Method NA gene sequences of 23 A（H3N2） influenza strains were acquired from NCBI database and lab isolations. According to sequence alignment results， specific TaqMan-MGB probes and primers were designed for R292K and N294S mutation sites in multiple realtime PCR method. The sensitivity was detected using the ten times dilution method. Clinical specimens， other subtypes of influenza and other common respiratory viruses were used to verify the specificity of the method．Results The designed probes could detect 107 （1000 copies） dilution of the corresponding template. Other subtypes of influenza virus and common respiratory viruses showed no cross reaction. Through the detection of 57 strains isolated from annual survey， no drug resistance mutations were found. Conclusions In this study，the A（H3N2） subtype influenza virus R292K and N294S mutation site TaqMan-MGB probe detection method bas been established. It is proved that the primer probe has high sensitivity and specificity with particular application potential.
［Abstract］Objective To establish a detection method to R292K and N294S mutation sites in H3N2 influenza A virus with TaqMan-MGB probes. Method NA gene sequences of 23 A（H3N2） influenza strains were acquired from NCBI database and lab isolations. According to sequence alignment results， specific TaqMan-MGB probes and primers were designed for R292K and N294S mutation sites in multiple realtime PCR method. The sensitivity was detected using the ten times dilution method. Clinical specimens， other subtypes of influenza and other common respiratory viruses were used to verify the specificity of the method．Results The designed probes could detect 107 （1000 copies） dilution of the corresponding template. Other subtypes of influenza virus and common respiratory viruses showed no cross reaction. Through the detection of 57 strains isolated from annual survey， no drug resistance mutations were found. Conclusions In this study，the A（H3N2） subtype influenza virus R292K and N294S mutation site TaqMan-MGB probe detection method bas been established. It is proved that the primer probe has high sensitivity and specificity with particular application potential.
2016, 37(10)
Abstract:
［Abstract］Objective To learn dietary and nutritional status of the toxic diffuse goiter（GD） patients in Yunnan region， to investigate dietary nutrition intake levels of GD patients. Methods By using food frequency method and self-programmed food frequency table， GD patients' dietary nutrition was investigated. We calculated GD patients' intake of all kinds of food and a variety of nutrients per capita per day， and compared them with the suggested values in Chinese residents balanced diet pagoda and dietary nutrients reference intakes（RNIs）; measured the patients' body weight， height and calculating body mass index in the field. Results （1）Dietary structure: GD patients' food intake kinds were various in Yunnan region，the intakes of cereals， tuber crops， meat， poultry， oils and fats and nuts were high; the intakes of vegetables， homonemeae， fruits， eggs， fish and shrimp， milk and milk products were low. （2）Energy and nutrient intake: the energy contribution from fats exceeded the recommended value， the energy contribution from carbohydrates was lower than the recommended value， the intakes of dietary fiber， vitamin A， thiamine， riboflavin， calcium and sodium were low， the intakes of vitamin E， phosphorus and iron were high. （3） Physical examination: GD patients' marasmus rate， overweight rate and obesity rate were 25.2%， 7.0% and 1.7%， respectively. There was no statistically significant difference between males and females（P＞0.05）. Conclusion GD patients' food intake kinds are various in Yunnan region， but the dietary structure is unreasonable.
［Abstract］Objective To learn dietary and nutritional status of the toxic diffuse goiter（GD） patients in Yunnan region， to investigate dietary nutrition intake levels of GD patients. Methods By using food frequency method and self-programmed food frequency table， GD patients' dietary nutrition was investigated. We calculated GD patients' intake of all kinds of food and a variety of nutrients per capita per day， and compared them with the suggested values in Chinese residents balanced diet pagoda and dietary nutrients reference intakes（RNIs）; measured the patients' body weight， height and calculating body mass index in the field. Results （1）Dietary structure: GD patients' food intake kinds were various in Yunnan region，the intakes of cereals， tuber crops， meat， poultry， oils and fats and nuts were high; the intakes of vegetables， homonemeae， fruits， eggs， fish and shrimp， milk and milk products were low. （2）Energy and nutrient intake: the energy contribution from fats exceeded the recommended value， the energy contribution from carbohydrates was lower than the recommended value， the intakes of dietary fiber， vitamin A， thiamine， riboflavin， calcium and sodium were low， the intakes of vitamin E， phosphorus and iron were high. （3） Physical examination: GD patients' marasmus rate， overweight rate and obesity rate were 25.2%， 7.0% and 1.7%， respectively. There was no statistically significant difference between males and females（P＞0.05）. Conclusion GD patients' food intake kinds are various in Yunnan region， but the dietary structure is unreasonable.
2016, 37(10)
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［Abstract］Objective To investigate the ability of dental service in Yunnan province by analyzing the distribution of oral medical institutions， the distribution of oraloral medical staffs， the per capita outpatient costs， and the rate of visiting the doctor. Methods A general survey on oral medical institutions was finished by using questionnaires．Results There were 1045 dental institutions，416 of them were public dental institutions. 305 were in Kunming. 305 dental institutions were in Kunming，the number of dental institutions per square kilometer was 14.2， the dental manpower index was15，the rate of visiting the doctor was 14.19%， per capita outpatient costs were 139.9 Yuan， and the above indexes were ranked first in Kunming．Conclusions In Yunnan province， oral health resources distribution were imbalanced. Dental service ability was uneven， perfect oral disease diagnosis and treatment quality control systems needs to be established，including cultivating specialized nurses relieve the tense situation of oral health care resources，and implenting the four hand operation，so as to effectively improve the quality of medical services.
［Abstract］Objective To investigate the ability of dental service in Yunnan province by analyzing the distribution of oral medical institutions， the distribution of oraloral medical staffs， the per capita outpatient costs， and the rate of visiting the doctor. Methods A general survey on oral medical institutions was finished by using questionnaires．Results There were 1045 dental institutions，416 of them were public dental institutions. 305 were in Kunming. 305 dental institutions were in Kunming，the number of dental institutions per square kilometer was 14.2， the dental manpower index was15，the rate of visiting the doctor was 14.19%， per capita outpatient costs were 139.9 Yuan， and the above indexes were ranked first in Kunming．Conclusions In Yunnan province， oral health resources distribution were imbalanced. Dental service ability was uneven， perfect oral disease diagnosis and treatment quality control systems needs to be established，including cultivating specialized nurses relieve the tense situation of oral health care resources，and implenting the four hand operation，so as to effectively improve the quality of medical services.
2016, 37(10)
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［Abstract］Objective To investigate the relationship between vaspin， Apelin， leptin and polycystic ovary syndrome. Methods We determined the serum vaspin，Apelin and leptin levels of 40 cases of PCOS patients and 30 cases of control group，and divided the PCOS group into three subgroups（normal weight， overweight and obesity）according to WHO criteria for obesity. Then we compared the serum vaspin，Apelin and leptin levels in PCOS and control group，and the differences in body weight index（body mass index，BMI） in women with polycystic ovary syndrome，and analyzed roles of adipokine vaspin，Apelin and leptin in the pathogenesis of PCOS. Results In PCOS patients adipokine vaspin，Apelin，leptin，insulin resistance index（Homeostasis model assessment for insulin index in resistence，HOMA-IR），fasting insulin （INS） and blood glucose，blood lipid were higher than those of the control group and increased with the increase of body mass index（BMI），and three kinds of adipokines and PCOS patients with HOMA-IR were positively correlated， there were statistically significant differences （P < 0.05）. Conclusion The results of vaspin， Apelin and leptin may be involved in the occurrence of PCOS.
［Abstract］Objective To investigate the relationship between vaspin， Apelin， leptin and polycystic ovary syndrome. Methods We determined the serum vaspin，Apelin and leptin levels of 40 cases of PCOS patients and 30 cases of control group，and divided the PCOS group into three subgroups（normal weight， overweight and obesity）according to WHO criteria for obesity. Then we compared the serum vaspin，Apelin and leptin levels in PCOS and control group，and the differences in body weight index（body mass index，BMI） in women with polycystic ovary syndrome，and analyzed roles of adipokine vaspin，Apelin and leptin in the pathogenesis of PCOS. Results In PCOS patients adipokine vaspin，Apelin，leptin，insulin resistance index（Homeostasis model assessment for insulin index in resistence，HOMA-IR），fasting insulin （INS） and blood glucose，blood lipid were higher than those of the control group and increased with the increase of body mass index（BMI），and three kinds of adipokines and PCOS patients with HOMA-IR were positively correlated， there were statistically significant differences （P < 0.05）. Conclusion The results of vaspin， Apelin and leptin may be involved in the occurrence of PCOS.
2016, 37(10)
Abstract:
［Abstract］Objective To compare clinical efficacy between microendoscopic discectomy （MED） and open discectomy（OD）in the treatment of patients with lumber intervertebral disc herniation （LIDH）. Methods 116 cases of LIDH who were given surgical treatment were selected as research subjects to retrospectively analyze their clinical data. Then，these cases were divided into MED group（n＝54） and OD group （n＝62） according to operation type. The curative efficacy， operation situation and incidence of complications were compared．Results All patients' operations were successful. After the one-year follow-up， the MED group had an excellent and good rate of 96.3%，which was statistically same with that of 93.5% in the OD group（P＞0.05）. In comparison with the OD group，MED group had a statistically shorter operation time， less bleeding volume in the operation， shorter length of incision and shorter rest time on bed after operation（all P＜0.05）. After operation， there's no case of severe complication，and the incidence of complications between the two groups was statistically same（P＞0.05）．Conclusions MED and OD are both effective operations for LIDH，which have high excellent and good rate. But in comparison with OD，MED has advantages of less damage and faster recovery.
［Abstract］Objective To compare clinical efficacy between microendoscopic discectomy （MED） and open discectomy（OD）in the treatment of patients with lumber intervertebral disc herniation （LIDH）. Methods 116 cases of LIDH who were given surgical treatment were selected as research subjects to retrospectively analyze their clinical data. Then，these cases were divided into MED group（n＝54） and OD group （n＝62） according to operation type. The curative efficacy， operation situation and incidence of complications were compared．Results All patients' operations were successful. After the one-year follow-up， the MED group had an excellent and good rate of 96.3%，which was statistically same with that of 93.5% in the OD group（P＞0.05）. In comparison with the OD group，MED group had a statistically shorter operation time， less bleeding volume in the operation， shorter length of incision and shorter rest time on bed after operation（all P＜0.05）. After operation， there's no case of severe complication，and the incidence of complications between the two groups was statistically same（P＞0.05）．Conclusions MED and OD are both effective operations for LIDH，which have high excellent and good rate. But in comparison with OD，MED has advantages of less damage and faster recovery.
2016, 37(10)
Abstract:
［Abstract］Objective To compare the clinical effect of vacuum sealing drainage and traditional methods on open tibial fractures of Gustilo grade-Ⅲ. Methods Seventy-eight patients with open tibial fractures of Gustilo grade-Ⅲ were divided into two groups， 43 patients in observation group were treated by vacuum sealing drainage， while 35 patients in control group were treated by traditional methods. The operative quality，postoperative recovery condition，clinical effect and rate of adverse reaction were analyzed between two groups．Results As for the hospital stays，healing time of wound and fracture， the observation group were significant shorter than control group （P<0.05）. The excellent and good rate of observation group was 83.72%，which was significantly higher than that of control group 65.71%（P＜0.01）. The adverse reaction rate of observation group was 6.98%，which was significantly lower than that of control group 17.14% （P＜0.05）．Conclusions Vacuum sealing drainage used for treatment of open tibial fractures of Gustilo grade-Ⅲ can shorten wound and fracture healing time，reduce the incidence of adverse reaction and improve the clinical effect. It is worth of popularization and application.
［Abstract］Objective To compare the clinical effect of vacuum sealing drainage and traditional methods on open tibial fractures of Gustilo grade-Ⅲ. Methods Seventy-eight patients with open tibial fractures of Gustilo grade-Ⅲ were divided into two groups， 43 patients in observation group were treated by vacuum sealing drainage， while 35 patients in control group were treated by traditional methods. The operative quality，postoperative recovery condition，clinical effect and rate of adverse reaction were analyzed between two groups．Results As for the hospital stays，healing time of wound and fracture， the observation group were significant shorter than control group （P<0.05）. The excellent and good rate of observation group was 83.72%，which was significantly higher than that of control group 65.71%（P＜0.01）. The adverse reaction rate of observation group was 6.98%，which was significantly lower than that of control group 17.14% （P＜0.05）．Conclusions Vacuum sealing drainage used for treatment of open tibial fractures of Gustilo grade-Ⅲ can shorten wound and fracture healing time，reduce the incidence of adverse reaction and improve the clinical effect. It is worth of popularization and application.
2016, 37(10)
Abstract:
［Abstract］Objective To investigate the effect of Celebrex on hemorheology and levels of serum intercelluar adhesion molecule-1（sICAM-1），serum vascular cell adhesion molecule-1（sVCAM-1），tumour necrosis factor-α（TNF-α），interleukin-6 （IL-6） in patients with delayed fracture healing. Methods 201 patients with delayed fracture healing in our hospital were randomly divided into observation group（n＝101）and control group （n＝100）. The patients in control group received conventional surgical internal fixation，while patients in observation group received conventional surgical internal fixation combined with Celebrex. The clinical efficacy was observed， while the changes of hemorheology and levels of sICAM-1， sVCAM-1， TNF-α， IL-6 were compared between two groups before and after treatment．Results The effective rate of observation group was 94.1%， which was significant higher than that of control group 70.0%（P＜0.01）. There were no significantly differences between two groups in the indexes of hemorheology and levels of sICAM-1，sVCAM-1，TNF-α，IL-6 before treatment（P＞0.05）. The indexes of hemorheology and levels of sICAM-1，sVCAM-1，TNF-α，IL-6 were significantly reduced after treatment， while the decreased degrees of observation group were better than those of control group，with statistically significant difference（P＜0.05）. Conclusions Celebrex can improve the indexes of hemorheology，reduce the levels of sICAM-1，sVCAM-1， TNF-α， IL-6 and improve the clinical effect in treatment of delayed fracture healing. It is worth of popularization and application.
［Abstract］Objective To investigate the effect of Celebrex on hemorheology and levels of serum intercelluar adhesion molecule-1（sICAM-1），serum vascular cell adhesion molecule-1（sVCAM-1），tumour necrosis factor-α（TNF-α），interleukin-6 （IL-6） in patients with delayed fracture healing. Methods 201 patients with delayed fracture healing in our hospital were randomly divided into observation group（n＝101）and control group （n＝100）. The patients in control group received conventional surgical internal fixation，while patients in observation group received conventional surgical internal fixation combined with Celebrex. The clinical efficacy was observed， while the changes of hemorheology and levels of sICAM-1， sVCAM-1， TNF-α， IL-6 were compared between two groups before and after treatment．Results The effective rate of observation group was 94.1%， which was significant higher than that of control group 70.0%（P＜0.01）. There were no significantly differences between two groups in the indexes of hemorheology and levels of sICAM-1，sVCAM-1，TNF-α，IL-6 before treatment（P＞0.05）. The indexes of hemorheology and levels of sICAM-1，sVCAM-1，TNF-α，IL-6 were significantly reduced after treatment， while the decreased degrees of observation group were better than those of control group，with statistically significant difference（P＜0.05）. Conclusions Celebrex can improve the indexes of hemorheology，reduce the levels of sICAM-1，sVCAM-1， TNF-α， IL-6 and improve the clinical effect in treatment of delayed fracture healing. It is worth of popularization and application.
2016, 37(10)
Abstract:
［Abstract］Objective To observe the safety and effectivity of the multimode combined treatment of domestic ultrapulsed CO2 laser on infacial atrophic acne scars. Methods Eighty patients with facial atrophic acne scars were randomly divided into Simple lattice model therapy group and multimode combinedultrapulsed CO2 laser group.The therapeutic effects and complication were observed and compared between two groups．Results The total effective rate in multimode combinedultrapulsed CO2 lasertherapy group was 95%， the total effective rate in Simple lattice model therapy group was 70%. There was a significant difference between two groups（ P＜0.05）. No permanent hyperpigmentation，depigmentation and obvious scar were observed. Conclusions Multimode combined treatment of domestic ultrapulsed CO2 laser is effective and safe in the treatment of facial atrophic acne scars. It is better than Simple lattice model therapy.
［Abstract］Objective To observe the safety and effectivity of the multimode combined treatment of domestic ultrapulsed CO2 laser on infacial atrophic acne scars. Methods Eighty patients with facial atrophic acne scars were randomly divided into Simple lattice model therapy group and multimode combinedultrapulsed CO2 laser group.The therapeutic effects and complication were observed and compared between two groups．Results The total effective rate in multimode combinedultrapulsed CO2 lasertherapy group was 95%， the total effective rate in Simple lattice model therapy group was 70%. There was a significant difference between two groups（ P＜0.05）. No permanent hyperpigmentation，depigmentation and obvious scar were observed. Conclusions Multimode combined treatment of domestic ultrapulsed CO2 laser is effective and safe in the treatment of facial atrophic acne scars. It is better than Simple lattice model therapy.
2016, 37(10)
Abstract:
［Abstract］Objective To detect the expression of Apelin，Vaspin and Leptin in serum， and to understand the relationship between the expression of Vaspin and Apelin，and the relationship between Leptin and I type of endometrial carcinoma. Methods We recorded the age，height，weight of patients， measured the blood pressure，and recorded the complete case data， and calculated the body mass index， then determined the serum Apelin and vaspin，leptin protein expression by enzyme linked immunosorbent assay（ELISA）. Results When 18.5≤BMI＜25 or BMI≥25， compared with the control group， the serum Apelin，vaspin，leptin of patients in the endometrial carcinoma group had statistically significant difference. In endometrial carcinoma group，the serum levels of three kinds of adipokines（Apelin and vaspin and leptin）had statistically significant difference between patients with 18.5≤BMI＜25 and BMI≥25. EC occurrence was correlated with BMI，diabetes，and high cholesterol hyperlipidemia，E2，HOMA-IR，serum Apelin，vaspin， leptin．Conclusions The patients with type I endometrial cancer express three kinds of fat factor，and the expression levels are significantly higher than the normal group. The occurrence of EC is related with BMI，diabetes，hyperlipidemia，E2，HOMA-IR，serum Apelin，Vaspin，Leptin.
［Abstract］Objective To detect the expression of Apelin，Vaspin and Leptin in serum， and to understand the relationship between the expression of Vaspin and Apelin，and the relationship between Leptin and I type of endometrial carcinoma. Methods We recorded the age，height，weight of patients， measured the blood pressure，and recorded the complete case data， and calculated the body mass index， then determined the serum Apelin and vaspin，leptin protein expression by enzyme linked immunosorbent assay（ELISA）. Results When 18.5≤BMI＜25 or BMI≥25， compared with the control group， the serum Apelin，vaspin，leptin of patients in the endometrial carcinoma group had statistically significant difference. In endometrial carcinoma group，the serum levels of three kinds of adipokines（Apelin and vaspin and leptin）had statistically significant difference between patients with 18.5≤BMI＜25 and BMI≥25. EC occurrence was correlated with BMI，diabetes，and high cholesterol hyperlipidemia，E2，HOMA-IR，serum Apelin，vaspin， leptin．Conclusions The patients with type I endometrial cancer express three kinds of fat factor，and the expression levels are significantly higher than the normal group. The occurrence of EC is related with BMI，diabetes，hyperlipidemia，E2，HOMA-IR，serum Apelin，Vaspin，Leptin.
2016, 37(10)
Abstract:
［Abstract］Objective To investigate the relationship between metalloproteinase-9 -9 （Matrix）， inhibitor-1 -1（MMP-9），metalloproteinase TIMP-1 factor（Matrix） and endothelial growth （VEGF）， and to investigate the correlation between the expression of（matrix） and vascular endothelial growth factor （endothelial）and vascular endothelial growth factor（vascular）. Methods Sixty-eight cases of non small cell lung cancer patients treated in fengning manchu autonomous county hospital from January to January 2014 were analyzed retrospectively， and the expression level of TIMP-1， VEGF and MMP-9 were analyzed by immunohistochemical method. Results The positive rates of MMP-9（75%），TIMP-1（19.12%）and VEGF （13.24%）were significantly higher than those in normal tissues（P＜0.05），MMP-9 （60.87%），VEGF （54.55%），TIMP-1 （65.22%） and VEGF （76.09%， 59.09%，63.64%）. The positive rates of TIMP-1，VEGF and MMP-9 were higher than those without lymph node metastasis（P＜0.05）. Conclusion MMP-9，TIMP-1 and VEGF expression levels can predict the invasion and metastasis of non small cell lung cancer，and the three kinds of substances can promote the development and progression of lung cancer in different degrees.
［Abstract］Objective To investigate the relationship between metalloproteinase-9 -9 （Matrix）， inhibitor-1 -1（MMP-9），metalloproteinase TIMP-1 factor（Matrix） and endothelial growth （VEGF）， and to investigate the correlation between the expression of（matrix） and vascular endothelial growth factor （endothelial）and vascular endothelial growth factor（vascular）. Methods Sixty-eight cases of non small cell lung cancer patients treated in fengning manchu autonomous county hospital from January to January 2014 were analyzed retrospectively， and the expression level of TIMP-1， VEGF and MMP-9 were analyzed by immunohistochemical method. Results The positive rates of MMP-9（75%），TIMP-1（19.12%）and VEGF （13.24%）were significantly higher than those in normal tissues（P＜0.05），MMP-9 （60.87%），VEGF （54.55%），TIMP-1 （65.22%） and VEGF （76.09%， 59.09%，63.64%）. The positive rates of TIMP-1，VEGF and MMP-9 were higher than those without lymph node metastasis（P＜0.05）. Conclusion MMP-9，TIMP-1 and VEGF expression levels can predict the invasion and metastasis of non small cell lung cancer，and the three kinds of substances can promote the development and progression of lung cancer in different degrees.
2016, 37(10)
Abstract:
［Abstract］Objective To detect the expression of HBV in human early embryos of infertility patients combined with hepatitis B virus（HBV）infection after undergoing in vitro fertilization and embryo transfer （IVF-ET） treatment，so as to provide reliable evidence for the vertical transmission of HBV through human germ cells. Methods We collected the abandoned early embryos of patients combined with HBV infection after undergoing IVF-ET treatment in the department of reproductive medicine of the First People's Hospital of Yunnan Province，total genomic DNA was obtained by single cell PCR，and then polymerase chain reaction（PCR）was used to detect the expression of HBV-DNA and covalently closed circular DNA of hepatitis B virus （HBV-cccDNA）in early embryos. Results Both HBV-DNA and HBV-cccDNA were detected in the early embryo among the mother or father or parents HBV infected patients. The positive detection rate of HBV-DNA in mother， father and parents HBV infected patients was 78.9%，76.3% and 100%，and the positive rate of HBV-cccDNA was 63.2%，78.9% and 100%. The difference between the mother and the father was not significant（P＞0.05）. However，the positive rate in parents HBV infected patients group was significantly higher than the mother or father group，showing a significant difference（P＜0.05）. Conclusion HBV can be transmitted from mother or father to early embryos via IVF-ET treament， and the risk of vertical transmission increases significantly when the couple are HBV infected.
［Abstract］Objective To detect the expression of HBV in human early embryos of infertility patients combined with hepatitis B virus（HBV）infection after undergoing in vitro fertilization and embryo transfer （IVF-ET） treatment，so as to provide reliable evidence for the vertical transmission of HBV through human germ cells. Methods We collected the abandoned early embryos of patients combined with HBV infection after undergoing IVF-ET treatment in the department of reproductive medicine of the First People's Hospital of Yunnan Province，total genomic DNA was obtained by single cell PCR，and then polymerase chain reaction（PCR）was used to detect the expression of HBV-DNA and covalently closed circular DNA of hepatitis B virus （HBV-cccDNA）in early embryos. Results Both HBV-DNA and HBV-cccDNA were detected in the early embryo among the mother or father or parents HBV infected patients. The positive detection rate of HBV-DNA in mother， father and parents HBV infected patients was 78.9%，76.3% and 100%，and the positive rate of HBV-cccDNA was 63.2%，78.9% and 100%. The difference between the mother and the father was not significant（P＞0.05）. However，the positive rate in parents HBV infected patients group was significantly higher than the mother or father group，showing a significant difference（P＜0.05）. Conclusion HBV can be transmitted from mother or father to early embryos via IVF-ET treament， and the risk of vertical transmission increases significantly when the couple are HBV infected.
2016, 37(10)
Abstract:
［Abstract］Objective To observe the effects of hypnosis therapy on rehabilitation of patients with spinal cord injury. Methods The data were randomly selected from 66 cases of patients with spinal cord injury treated in our hospital from November 2011 - November 2012. According to the treatment，the patients were divided into research group and control group. Control patients were given routine therapy，and patients in the research group were given hypnosis therapy， we observed and compared the recovery of two groups of patients after treatment. Results After treatment， the spinal cord function score（SCIM）of patients in the research group was better than control group， the difference was statistically significant（P < 0.05）. After treatment，the depression and anxiety scale （HAMD， HAMA） of patients in the research group was better than control group，the difference had statistical significance（P < 0.05）. The quality of life after treatment of research group patients（WHOQOL BREF）was better than control group，the difference had statistical significance（P < 0.05）. Conclusion Hypnosis therapy helps to improve depression and anxiety in patients with spinal cord injury， improve patients with spinal cord function independently， should be widely used in clinical treatment and promotion.
［Abstract］Objective To observe the effects of hypnosis therapy on rehabilitation of patients with spinal cord injury. Methods The data were randomly selected from 66 cases of patients with spinal cord injury treated in our hospital from November 2011 - November 2012. According to the treatment，the patients were divided into research group and control group. Control patients were given routine therapy，and patients in the research group were given hypnosis therapy， we observed and compared the recovery of two groups of patients after treatment. Results After treatment， the spinal cord function score（SCIM）of patients in the research group was better than control group， the difference was statistically significant（P < 0.05）. After treatment，the depression and anxiety scale （HAMD， HAMA） of patients in the research group was better than control group，the difference had statistical significance（P < 0.05）. The quality of life after treatment of research group patients（WHOQOL BREF）was better than control group，the difference had statistical significance（P < 0.05）. Conclusion Hypnosis therapy helps to improve depression and anxiety in patients with spinal cord injury， improve patients with spinal cord function independently， should be widely used in clinical treatment and promotion.
2016, 37(10)
Abstract:
［Abstract］Objective To observe the clinical curative effect of computer aided cognitive rehabilitation system for the treatment of cognitive impairment after stroke. Methods The data of 70 cases of cognitive impairment after stroke patients were randomly selected from November 2011 - November 2012 in our hospital， according to the treatment method，patients were randomly divided into research group（n＝35） and control group （n＝35）. The control group patients were given cognitive training， sports and drugs and other conventional treatment. On the basis of conventional treatment ， the research group patients were given computer aided cognitive rehabilitation system treatment. Then we observed and compared the clinical curative effect of two groups of patients after treatment. Results The cognitive rating scale（LOTCA）scores in patients in the research group was better than that in the control group， the difference was statistically significant（P < 0.05）. The simple mental state examination （MMSE） score of patients in the research group was better than that in the control group， the difference was statistically significant（P < 0.05）. Conclusion Computer assisted cognitive rehabilitation system for patients with cognitive impairment after stroke has significant curative effect， and should be popularized and widely used in clinical treatment.
［Abstract］Objective To observe the clinical curative effect of computer aided cognitive rehabilitation system for the treatment of cognitive impairment after stroke. Methods The data of 70 cases of cognitive impairment after stroke patients were randomly selected from November 2011 - November 2012 in our hospital， according to the treatment method，patients were randomly divided into research group（n＝35） and control group （n＝35）. The control group patients were given cognitive training， sports and drugs and other conventional treatment. On the basis of conventional treatment ， the research group patients were given computer aided cognitive rehabilitation system treatment. Then we observed and compared the clinical curative effect of two groups of patients after treatment. Results The cognitive rating scale（LOTCA）scores in patients in the research group was better than that in the control group， the difference was statistically significant（P < 0.05）. The simple mental state examination （MMSE） score of patients in the research group was better than that in the control group， the difference was statistically significant（P < 0.05）. Conclusion Computer assisted cognitive rehabilitation system for patients with cognitive impairment after stroke has significant curative effect， and should be popularized and widely used in clinical treatment.
2016, 37(10)
Abstract:
［Abstract］Objective To summarize the clinical experience of three different treatments for gallbladder and common bile duct stones. Methods The clinical data of 180 cases of gallbladder stones combined with bile duct stones undergoing surgery from May 2010 to May 2013 were retrospectively analyzed. They were divided into three groups， A group of 60 patients underwent a period of endoscopic sphincterotomy（EST），under the second phase of laparoscopic cholecystectomy（LC）；Group B 60 patients underwent laparoscopic cholecystectomy（LC）+ laparoscopic common bile duct exploration surgery（LCBDE）+ T tube drainage；Group C 60 patients underwent conventional open cholecystectomy（OC）+ bile duct exploration（OCBDE）+ T tube drainage. Results A group of 53 cases completed surgery successfully，5 cases of remaining 7 patients failured in the first phase surgery，2 cases of the 5 patients did LC + LCBDE，3 of the 5 patients underwent conventional surgery. Two patients underwent the conventional surgery in the second phase surgery. B group of 57 cases completed surgery successfully，three cases convert to open surgery. All of the group C completed the surgery successfully. Group A complication was the most in the three groups（P＜0.05）；group B had the shortest time of hospitalization（P＜0.05），the complication rate was lower than that in group A（P＜0.05），the complication had no significant difference between A and B.（Pand group B > 0.05），group B had shortest operation time（P＜0.05）；no statistically significant differences were found among three groups in fasting time. Conclusion Three treatment methods have advantages as well as disadvantages， a reasonable treatment should be selected according to the specific circumstances.
［Abstract］Objective To summarize the clinical experience of three different treatments for gallbladder and common bile duct stones. Methods The clinical data of 180 cases of gallbladder stones combined with bile duct stones undergoing surgery from May 2010 to May 2013 were retrospectively analyzed. They were divided into three groups， A group of 60 patients underwent a period of endoscopic sphincterotomy（EST），under the second phase of laparoscopic cholecystectomy（LC）；Group B 60 patients underwent laparoscopic cholecystectomy（LC）+ laparoscopic common bile duct exploration surgery（LCBDE）+ T tube drainage；Group C 60 patients underwent conventional open cholecystectomy（OC）+ bile duct exploration（OCBDE）+ T tube drainage. Results A group of 53 cases completed surgery successfully，5 cases of remaining 7 patients failured in the first phase surgery，2 cases of the 5 patients did LC + LCBDE，3 of the 5 patients underwent conventional surgery. Two patients underwent the conventional surgery in the second phase surgery. B group of 57 cases completed surgery successfully，three cases convert to open surgery. All of the group C completed the surgery successfully. Group A complication was the most in the three groups（P＜0.05）；group B had the shortest time of hospitalization（P＜0.05），the complication rate was lower than that in group A（P＜0.05），the complication had no significant difference between A and B.（Pand group B > 0.05），group B had shortest operation time（P＜0.05）；no statistically significant differences were found among three groups in fasting time. Conclusion Three treatment methods have advantages as well as disadvantages， a reasonable treatment should be selected according to the specific circumstances.
2016, 37(10)
Abstract:
［摘要］目的 探讨同型半胱氨酸水平（Hcy）和超敏C 反应蛋白（hs-CRP）与阿尔茨海默病（AD）病临床表现精神行为的关系．方法 选择云南省第三人民医院2014 年1 月至2016 年1 月住院的AD 患者20例，以酶法检测AD患者治疗前后血浆Hcy 与hs-CRP 水平，阿尔茨海默病病理行为（BEHAVE-AD） 评定量表评估患者治疗效果，分析Hcy 与hs-CRP水平与AD患者精神行为症状的相关性．结果 AD 患者的精神行为评分与血浆Hcy、hs-CRP浓度治疗前后水平对比均存在差异（P＜0.01）．结论 Hcy，CRP浓度与AD患者精神行为有相关性.
2016, 37(10)
Abstract:
［Abstract］Objective To explore the application value of DOSE index score in the peitients with chronic obstructive pulmonary disease．Methods 122 cases of plateau COPD patients were followed up for 12 months， and we recorded and analyzed the patient's health and life. We also recorde FEV1 and DOSE scores of the patients with COPD，and record the COPD risk events，including the number of respiratory failure and death，and the times of hospitalization， total such confinement， outpatient expenses，hospitalization expenses，mMRC，and scored in the number of exacerbations，etc．Results The DOSE index score was negatively correlated with FEV1 % pred （r＝0.73，P ＜ 0.05） for 122 COPD patients， and were positively correlated with mMRC （r＝085，P ＜ 0.01），the annual number of exacerbations （r＝0.71，P ＜ 0.01）， respiratory failure （r＝0.65，P ＜ 0.01）， heart failure （r＝0.50，P ＜ 0.01）， number of outpatient service （r＝0.12，P＜0.01）， hospitalization time （r＝0.70， P＜ 0.01）， the total such confinement （r＝0.66， P＜0.01）， outpatient expenses （r＝0.13， P > 0.13）， hospitalization expenses（r＝0.65， P＜0.01）. ROC curve was used to analyzed the cut-off point and curve area of COPD DOSE index．Conclusion The DOSE index is a simple COPD assessment tools， and is closely related to the prognosis of patients and health.
［Abstract］Objective To explore the application value of DOSE index score in the peitients with chronic obstructive pulmonary disease．Methods 122 cases of plateau COPD patients were followed up for 12 months， and we recorded and analyzed the patient's health and life. We also recorde FEV1 and DOSE scores of the patients with COPD，and record the COPD risk events，including the number of respiratory failure and death，and the times of hospitalization， total such confinement， outpatient expenses，hospitalization expenses，mMRC，and scored in the number of exacerbations，etc．Results The DOSE index score was negatively correlated with FEV1 % pred （r＝0.73，P ＜ 0.05） for 122 COPD patients， and were positively correlated with mMRC （r＝085，P ＜ 0.01），the annual number of exacerbations （r＝0.71，P ＜ 0.01）， respiratory failure （r＝0.65，P ＜ 0.01）， heart failure （r＝0.50，P ＜ 0.01）， number of outpatient service （r＝0.12，P＜0.01）， hospitalization time （r＝0.70， P＜ 0.01）， the total such confinement （r＝0.66， P＜0.01）， outpatient expenses （r＝0.13， P > 0.13）， hospitalization expenses（r＝0.65， P＜0.01）. ROC curve was used to analyzed the cut-off point and curve area of COPD DOSE index．Conclusion The DOSE index is a simple COPD assessment tools， and is closely related to the prognosis of patients and health.
2016, 37(10)
Abstract:
［Abstract］Objective To study the influence of Naikan therapy on depression in patients with Schizophrenia. Methods 256 patients with Schizophrenia were divided into study group and control group according to the set sequence. The study group was simulated by Naikan therapy and the control group was given routine cure. All patients were assessed by Positive and negative symptom scale（PANSS），Simple coping style questionnaire （SCSQ），Defense way questionnaire（DSQ）， Hamilton depression scale （HAMD）， and Hamilton anxiety scale （HAMA）. Results In the 6th week，the differences in HAMD score，Cover factor， negative coping were statistically significant between the two groups（t＝2.37，2.33，2.36，P＜0.05）. The differencs in HAMA score， Cover factor，negative coping were statistically significant between the 0th and the 6 th week in the study groups （t =2.31，2.42，2.47，P < 0.05）. The difference of PANSS score were statistically significant between the 0th and 6th week of the two groups. Conclusion The Naikan therapy can improve the mental symptoms and depressive symptoms in patients with Schizophrenia.
［Abstract］Objective To study the influence of Naikan therapy on depression in patients with Schizophrenia. Methods 256 patients with Schizophrenia were divided into study group and control group according to the set sequence. The study group was simulated by Naikan therapy and the control group was given routine cure. All patients were assessed by Positive and negative symptom scale（PANSS），Simple coping style questionnaire （SCSQ），Defense way questionnaire（DSQ）， Hamilton depression scale （HAMD）， and Hamilton anxiety scale （HAMA）. Results In the 6th week，the differences in HAMD score，Cover factor， negative coping were statistically significant between the two groups（t＝2.37，2.33，2.36，P＜0.05）. The differencs in HAMA score， Cover factor，negative coping were statistically significant between the 0th and the 6 th week in the study groups （t =2.31，2.42，2.47，P < 0.05）. The difference of PANSS score were statistically significant between the 0th and 6th week of the two groups. Conclusion The Naikan therapy can improve the mental symptoms and depressive symptoms in patients with Schizophrenia.
2016, 37(10)
Abstract:
［Abstract］Objective To compare the consistency of the results of clinical specimens between Tecan Freedom Evolyzer-2200 automated enzyme immunoassay analyzer and manual ELISA method， so as to explore more scientific and reliable test method. Methods Ninety samples were randomly selected from clinical and physical examination，the IMARK2 microplate reader and the instrument operating manual sample measurement was used to test hepatitis A，hepatitis B 5， hepatitis C，hepatitis E 2，TP and HIV，and the results were given correlation and linear regression analysis. Results For the test items of HBsAg，HBsAb， HBeAg， TP， HIV， the correlation coefficient was r2> 0.9，the data showed a good correlation，and for HAV-IgM， HBeAb， HBcAb， HCV，HEV-IgM，HEV-IgG，the correlation coefficient was 0.9> r 2 > 0.6. Compared with manual method， the total coincidence rate of automated enzyme immunoassay analyzer was 96.9% in 11 indexes of 90 samples. Conclusions Automatic and manual ELISA method has a good correlation. Automatic ELISA also greatly reduce the labor burden of laboratory personnel， improve efficiency， should be strengthened in clinical practice.
［Abstract］Objective To compare the consistency of the results of clinical specimens between Tecan Freedom Evolyzer-2200 automated enzyme immunoassay analyzer and manual ELISA method， so as to explore more scientific and reliable test method. Methods Ninety samples were randomly selected from clinical and physical examination，the IMARK2 microplate reader and the instrument operating manual sample measurement was used to test hepatitis A，hepatitis B 5， hepatitis C，hepatitis E 2，TP and HIV，and the results were given correlation and linear regression analysis. Results For the test items of HBsAg，HBsAb， HBeAg， TP， HIV， the correlation coefficient was r2> 0.9，the data showed a good correlation，and for HAV-IgM， HBeAb， HBcAb， HCV，HEV-IgM，HEV-IgG，the correlation coefficient was 0.9> r 2 > 0.6. Compared with manual method， the total coincidence rate of automated enzyme immunoassay analyzer was 96.9% in 11 indexes of 90 samples. Conclusions Automatic and manual ELISA method has a good correlation. Automatic ELISA also greatly reduce the labor burden of laboratory personnel， improve efficiency， should be strengthened in clinical practice.
2016, 37(10)
Abstract:
［Abstract］Objective To investigate the effects of preoperative anxiety on the recovery of anesthesia and postoperative analgesia in patients undergoing elective laparoscopic ovarian cystectomy. Methods A total of 87 patients were enrolled who were undergoing elective laparoscopic ovarian cystectomy during July 2015 to June 2016. State-Trait Anxiety Inventory-1 was administered to the patients: patients with anxiety were included in the high-anxiety group（Group H）and patients witouth anxiety were included in the low-anxiety group（Group L）. We observed the recovery of anesthesia and postoperative analgesia of the patients in two groups．Results In group H，the extubation time，the time for the Aldrete score to reach 9，were significantly longer；more agitation were found；and more fentanyl and parecoxib were needed. The difference was statistically significant（P＜0.05）. The age，BMI，operation time，anesthesia time of the patients in the two groups had no statistically significant differences（P＞0.05）. Conclusion High anxiety has a negative effect on recovery from anesthesia and on postoperative pain control in patients undergoing elective laparoscopic ovarian cystectomy.
［Abstract］Objective To investigate the effects of preoperative anxiety on the recovery of anesthesia and postoperative analgesia in patients undergoing elective laparoscopic ovarian cystectomy. Methods A total of 87 patients were enrolled who were undergoing elective laparoscopic ovarian cystectomy during July 2015 to June 2016. State-Trait Anxiety Inventory-1 was administered to the patients: patients with anxiety were included in the high-anxiety group（Group H）and patients witouth anxiety were included in the low-anxiety group（Group L）. We observed the recovery of anesthesia and postoperative analgesia of the patients in two groups．Results In group H，the extubation time，the time for the Aldrete score to reach 9，were significantly longer；more agitation were found；and more fentanyl and parecoxib were needed. The difference was statistically significant（P＜0.05）. The age，BMI，operation time，anesthesia time of the patients in the two groups had no statistically significant differences（P＞0.05）. Conclusion High anxiety has a negative effect on recovery from anesthesia and on postoperative pain control in patients undergoing elective laparoscopic ovarian cystectomy.
2016, 37(10)
Abstract:
［Abstract］Objective To investigate the effect of the balloon Bionic midwifery on the delivery outcomes and to analyze the clinical value. Methods 1 683 parturients from June 2014 to May 2015 were selected. They were randomly divided into observation group（832 cases，applied balloon Bionic Midwifery ）and control group 851 cases . We compared the labor， the postpartum hemorrhage， the outcomes of pregnancy and the rate of survival of neonates of the two groups．Results The first and second stage as well as the total stage of labors of the observation group were lower than the control group（P＜0.01）；Also， the rate of cesarean delivery and the hemorrhage together with the asphyxia of neonates were lower than the control group （P＜0.01） .However，the rate of vaginal delivery was higher than the control group（P＜0.05）.The differences between them had a great statistical significance. Conclusions The balloon bionic midwifery technology has an advantage in reducing the rate of cesarean delivery and the maternal pain of pregnant women as well as the maternal complications. It is an effective and safe midwifery technology. So it has a great value of spreading in clinical trials.
［Abstract］Objective To investigate the effect of the balloon Bionic midwifery on the delivery outcomes and to analyze the clinical value. Methods 1 683 parturients from June 2014 to May 2015 were selected. They were randomly divided into observation group（832 cases，applied balloon Bionic Midwifery ）and control group 851 cases . We compared the labor， the postpartum hemorrhage， the outcomes of pregnancy and the rate of survival of neonates of the two groups．Results The first and second stage as well as the total stage of labors of the observation group were lower than the control group（P＜0.01）；Also， the rate of cesarean delivery and the hemorrhage together with the asphyxia of neonates were lower than the control group （P＜0.01） .However，the rate of vaginal delivery was higher than the control group（P＜0.05）.The differences between them had a great statistical significance. Conclusions The balloon bionic midwifery technology has an advantage in reducing the rate of cesarean delivery and the maternal pain of pregnant women as well as the maternal complications. It is an effective and safe midwifery technology. So it has a great value of spreading in clinical trials.
2016, 37(10)
Abstract:
［Abstract］Objective To observe and analyze the clinical effect of laparoscopic assisted vaginal hysterectomy and vaginal hysterectomy for non prolapse of uterus．Methods From March 2015 to April 2016， 126 cases of uterus benign lesions received surgical treatment in our hospital were selected and divided into observation group and control group. The observation group was given laparoscopic assisted vaginal hysterectomy resection，while the control group was given routine vaginal hysterectomy. The blood loss，operative time，hospitalization time and complications incidence were compared between two groups. Results The operation time and the amount of bleeding in the observation group were significantly higher than that in the control group，the difference was statistically significant （P < 0.05）. The length of stay in the observation group was significantly shorter than that in the control group， the difference was statistically significant （P < 0.05）. There was no significant difference in the incidence of surgical complications between the two groups （P > 0.05）. The quality of life of the two groups was compared with the total score， the observation group was higher （230.79 + 9.54）， P < 0.05， the difference was statistically significant. Conclusion Laparoscopic assisted vaginal hysterectomy and vaginal hysterectomy in the treatment of non prolapse uterus has a good clinical effect， should be chosen and applied based on the actual situation of patients.
［Abstract］Objective To observe and analyze the clinical effect of laparoscopic assisted vaginal hysterectomy and vaginal hysterectomy for non prolapse of uterus．Methods From March 2015 to April 2016， 126 cases of uterus benign lesions received surgical treatment in our hospital were selected and divided into observation group and control group. The observation group was given laparoscopic assisted vaginal hysterectomy resection，while the control group was given routine vaginal hysterectomy. The blood loss，operative time，hospitalization time and complications incidence were compared between two groups. Results The operation time and the amount of bleeding in the observation group were significantly higher than that in the control group，the difference was statistically significant （P < 0.05）. The length of stay in the observation group was significantly shorter than that in the control group， the difference was statistically significant （P < 0.05）. There was no significant difference in the incidence of surgical complications between the two groups （P > 0.05）. The quality of life of the two groups was compared with the total score， the observation group was higher （230.79 + 9.54）， P < 0.05， the difference was statistically significant. Conclusion Laparoscopic assisted vaginal hysterectomy and vaginal hysterectomy in the treatment of non prolapse uterus has a good clinical effect， should be chosen and applied based on the actual situation of patients.
2016, 37(10)
Abstract:
［Abstract］Objective To investigate the clinical characteristics and surgical methods of children with thyroglossal duct cyst （TDC）. Methods A retrospective analysis was made on the clinical data and imaging characteristics from 98 cases of children with primary thyroglossal duct cyst and 40 cases of children with recurrent thyroglossal duct cyst during the time 2012 to 2015 in Kunming children's Hospital's Otolaryngology Head and Neck Department. For new-onset cases， the classic Sistrunk operation method was chosen；for postoperative recurrence cases，the enlarged Sistrunk operation method was chosen．Results Thyroglossal duct cyst CT appeared single cystic lesion and polycystic lesions; the single cystic lesion was located under the level of the hyoid bone and on the level of the hyoid bone; polycystic lesions were located in the upper and lower hyoid bone，and highly related to hyoid and thyroid；B ultrasonic results showed low echo or heterogeneous echo，no substance，with posterior echo enhancement. Sistrunk operation and the enlarged Sistrunk operation treatment efficacy was positive for new-onset cases and postoperative recurrence patients. No recurrence was found in 2 years of follow-up. Conclusions The pathogenic and anatomical characteristics of thyroglossal duct cyst should be fully understood. The hyoid bone and the surrounding area of the glossal blind aperture in the base of the tongue are the focal point of surgical operation; ring ligation is the key after resection of the diseased tissue.
［Abstract］Objective To investigate the clinical characteristics and surgical methods of children with thyroglossal duct cyst （TDC）. Methods A retrospective analysis was made on the clinical data and imaging characteristics from 98 cases of children with primary thyroglossal duct cyst and 40 cases of children with recurrent thyroglossal duct cyst during the time 2012 to 2015 in Kunming children's Hospital's Otolaryngology Head and Neck Department. For new-onset cases， the classic Sistrunk operation method was chosen；for postoperative recurrence cases，the enlarged Sistrunk operation method was chosen．Results Thyroglossal duct cyst CT appeared single cystic lesion and polycystic lesions; the single cystic lesion was located under the level of the hyoid bone and on the level of the hyoid bone; polycystic lesions were located in the upper and lower hyoid bone，and highly related to hyoid and thyroid；B ultrasonic results showed low echo or heterogeneous echo，no substance，with posterior echo enhancement. Sistrunk operation and the enlarged Sistrunk operation treatment efficacy was positive for new-onset cases and postoperative recurrence patients. No recurrence was found in 2 years of follow-up. Conclusions The pathogenic and anatomical characteristics of thyroglossal duct cyst should be fully understood. The hyoid bone and the surrounding area of the glossal blind aperture in the base of the tongue are the focal point of surgical operation; ring ligation is the key after resection of the diseased tissue.
2016, 37(10)
Abstract:
［Abstract］Objective To explore the clinical value of modular flexible ureteroscope combined with holmium laser lithotripsy in the treatment of renal calculi. Methods From November 2013 to June 2015，72 patients with renal calculi were treated by modular flexible ureteroscope（Polydiagnost，German）combined with holmium laser lithotripsy. The diameter of stones was 10-45 mm，16 mm on average. Twenty cases were indwelling double J tube for 3-4 weeks before operation. 200 μm holmium laser fiber was used and laser power was set to 1.5J/10-20Hz（5 to 30 W）. KUB examination was performed 1 month after operation．Results Seventy-two cases were treated. F12 ureter sheath was successfully inserted into ureter in 56 cases， and failed in 16 cases. In 6 cases，modular flexible ureteroscope was put into renal pelvis and calices with the help of guidewire，and stones were successfully treated. MPCNL was selected to treat calculi in 6 cases，and modular flexible ureteroscope lithotripsy was successfully applid after double J tubes were placed for 4 weeks in 4 cases. Lower renal calyceal calculi were not found in 6 cases cases，ESWL was selected to treat these cases after double J tube indwelling. The stone found rate was 90.9%（60/66）. KUB was examined 1 month after operation. Residul stones were found in 5 cases. Total free rate was 91.7%（55/60）through single operation. Calculus lines up clean the zone leading 95% credibilities is 84.84%-98.56%. The time of lithotripsy was 20-150 min，and the average time was 50 min. During operation， perfusion fluid extravasation and peritoneal effusion were found in 1 case. No severe complications such as heavy bleeding， ureteral avulsion or perforation， urinary tract infection were found in all cases. The average hospitalization was 3 days after modular flexible ureteroscope．Conclusions Modular flexible ureteroscope is easy to operate， and the usage cost is lower. The technology， modular flexible ureteroscope combined with holmium laser lithotripsy for treatment of renal calculi， has the advantages of minimal invasion，high safety and effectiveness， less complications. So this technology is worthy of clinical popularization and application.
［Abstract］Objective To explore the clinical value of modular flexible ureteroscope combined with holmium laser lithotripsy in the treatment of renal calculi. Methods From November 2013 to June 2015，72 patients with renal calculi were treated by modular flexible ureteroscope（Polydiagnost，German）combined with holmium laser lithotripsy. The diameter of stones was 10-45 mm，16 mm on average. Twenty cases were indwelling double J tube for 3-4 weeks before operation. 200 μm holmium laser fiber was used and laser power was set to 1.5J/10-20Hz（5 to 30 W）. KUB examination was performed 1 month after operation．Results Seventy-two cases were treated. F12 ureter sheath was successfully inserted into ureter in 56 cases， and failed in 16 cases. In 6 cases，modular flexible ureteroscope was put into renal pelvis and calices with the help of guidewire，and stones were successfully treated. MPCNL was selected to treat calculi in 6 cases，and modular flexible ureteroscope lithotripsy was successfully applid after double J tubes were placed for 4 weeks in 4 cases. Lower renal calyceal calculi were not found in 6 cases cases，ESWL was selected to treat these cases after double J tube indwelling. The stone found rate was 90.9%（60/66）. KUB was examined 1 month after operation. Residul stones were found in 5 cases. Total free rate was 91.7%（55/60）through single operation. Calculus lines up clean the zone leading 95% credibilities is 84.84%-98.56%. The time of lithotripsy was 20-150 min，and the average time was 50 min. During operation， perfusion fluid extravasation and peritoneal effusion were found in 1 case. No severe complications such as heavy bleeding， ureteral avulsion or perforation， urinary tract infection were found in all cases. The average hospitalization was 3 days after modular flexible ureteroscope．Conclusions Modular flexible ureteroscope is easy to operate， and the usage cost is lower. The technology， modular flexible ureteroscope combined with holmium laser lithotripsy for treatment of renal calculi， has the advantages of minimal invasion，high safety and effectiveness， less complications. So this technology is worthy of clinical popularization and application.
2016, 37(10)
Abstract:
［Abstract］Objective To evaluate the application value of bedside lung ultrasound in diagnosis of neonatal severe pneumonia．Methods Bedside lung ultrasound and chest X-ray were performed in 59 the newborn patients admitted to the newborn department for suspected severe pneumonia，from October 2015 to March 2016．According to the characteristics of the chest X-ray，patients were divided into consolidation group（widely spot shadow in double lung and airbronchograms）and the unconsolidation group（ enlargement，fuzzy texture in double lung or small patch sample changes）．Differences in ultrasonic characteristics were compared between the two groups． Results Ultrasonic image characteristics were pulmonary consolidation with air bronchograms，A-line disappearance，pleural line abnormalities. Among 23 patients with chest X-ray，21 patients were found pulmonary consolidation by lung ultrasound，and all 36 patients in unconsolidation group were not found pulmonary consolidation by lung ultrasound，and there was no significant difference in the detection rate by lung ultrasound between the two groups（P＞0.05）．The pulmonary ultrasound results of 20 cases in the consolidation group showed A-line disappearance，37 cases in the unconsolidation group showed A-line disappearance. There was no statistical significant differences between the two groups（P＞0.05）. The pulmonary ultrasound results showed 11 cases in the consolidation group and 15 cases in the unconsolidation group with pleural line abnormalities， there was no statistically significant differences between the two groups（ P＞0.05 ）. Conclusions Ultrasound image characteristics of neonatal severe pneumonia include pulmonary consolidation and air bronchograms，A-line disappearance，pleural line abnormalities. Compared lung ultrasound and chest X-ray result，there was no difference between consolidation group and unconsolidation group. Both of the two methods can be used for evaluating the diagnosis of pneumonia.
［Abstract］Objective To evaluate the application value of bedside lung ultrasound in diagnosis of neonatal severe pneumonia．Methods Bedside lung ultrasound and chest X-ray were performed in 59 the newborn patients admitted to the newborn department for suspected severe pneumonia，from October 2015 to March 2016．According to the characteristics of the chest X-ray，patients were divided into consolidation group（widely spot shadow in double lung and airbronchograms）and the unconsolidation group（ enlargement，fuzzy texture in double lung or small patch sample changes）．Differences in ultrasonic characteristics were compared between the two groups． Results Ultrasonic image characteristics were pulmonary consolidation with air bronchograms，A-line disappearance，pleural line abnormalities. Among 23 patients with chest X-ray，21 patients were found pulmonary consolidation by lung ultrasound，and all 36 patients in unconsolidation group were not found pulmonary consolidation by lung ultrasound，and there was no significant difference in the detection rate by lung ultrasound between the two groups（P＞0.05）．The pulmonary ultrasound results of 20 cases in the consolidation group showed A-line disappearance，37 cases in the unconsolidation group showed A-line disappearance. There was no statistical significant differences between the two groups（P＞0.05）. The pulmonary ultrasound results showed 11 cases in the consolidation group and 15 cases in the unconsolidation group with pleural line abnormalities， there was no statistically significant differences between the two groups（ P＞0.05 ）. Conclusions Ultrasound image characteristics of neonatal severe pneumonia include pulmonary consolidation and air bronchograms，A-line disappearance，pleural line abnormalities. Compared lung ultrasound and chest X-ray result，there was no difference between consolidation group and unconsolidation group. Both of the two methods can be used for evaluating the diagnosis of pneumonia.
2016, 37(10)
Abstract:
［Abstract］Objective To evaluate the clinical efficacy of Tanreqing injection combined with ganciclovir in the treatment of pediatric respiratory syncytial virus pneumonia. Methods Ninety cases of children with respiratory tract virus pneumonia were selected in our hospital from August 2014 to December 2015， and were randomly divided into observation group and control group，and 45 cases in each group. The control group was given conventional anti infection，expectorant and antiasthmatic，antipyretic，atomization inhalation symptomatic and supportive treatment，on this basis the ganciclovir injection；the observation group was additionally given Tanreqing treatment on the basis of the control group. Then we compared the effects of two groups，pulmonary rales， wheezing，cough disappeared time， hospitalization time and the incidence of adverse reactions. Results After the treatment，the total efficiency of the observation group （93.3%） was significantly higher than that of control group （75.6%），the difference was statistically significant （P < 0.05）. The pulmonary rales （5.58 + 1.41），cough （5.78 + 1.61），wheezing（3.45 + 0.87）disappear time and hospital stay time（5.34± + 0.61）of patients in the observation group was significantly shorter than that of the control group（6.93 + 1.61，7.73 + 1.65，479 + 1.02， 736，0.52），and the difference was statistically significant（P < 0.05）. The adverse reaction incidence in the observation group（20.0%）and the control group（22.2%）had no statistically significant difference（P > 0.05）. Conclusion Tanreqing injection combined with ganciclovir in the treatment of children with respiratory syncytial virus pneumonia has good efficacy and less adverse reactions， it is worthy of further research and application in clinic.
［Abstract］Objective To evaluate the clinical efficacy of Tanreqing injection combined with ganciclovir in the treatment of pediatric respiratory syncytial virus pneumonia. Methods Ninety cases of children with respiratory tract virus pneumonia were selected in our hospital from August 2014 to December 2015， and were randomly divided into observation group and control group，and 45 cases in each group. The control group was given conventional anti infection，expectorant and antiasthmatic，antipyretic，atomization inhalation symptomatic and supportive treatment，on this basis the ganciclovir injection；the observation group was additionally given Tanreqing treatment on the basis of the control group. Then we compared the effects of two groups，pulmonary rales， wheezing，cough disappeared time， hospitalization time and the incidence of adverse reactions. Results After the treatment，the total efficiency of the observation group （93.3%） was significantly higher than that of control group （75.6%），the difference was statistically significant （P < 0.05）. The pulmonary rales （5.58 + 1.41），cough （5.78 + 1.61），wheezing（3.45 + 0.87）disappear time and hospital stay time（5.34± + 0.61）of patients in the observation group was significantly shorter than that of the control group（6.93 + 1.61，7.73 + 1.65，479 + 1.02， 736，0.52），and the difference was statistically significant（P < 0.05）. The adverse reaction incidence in the observation group（20.0%）and the control group（22.2%）had no statistically significant difference（P > 0.05）. Conclusion Tanreqing injection combined with ganciclovir in the treatment of children with respiratory syncytial virus pneumonia has good efficacy and less adverse reactions， it is worthy of further research and application in clinic.
2016, 37(10)
Abstract:
［Abstract］GCC/PKG/VASP，PKA/VASP et.al are associated with the occurrence and development of colorectal cancer. As one of the important signaling molecules of above signal pathways，VASP has constributed to the invasion，metastasis，apoptosis，angiogenesis and other events of colorectal cancer. The role of VASP signaling pathways in the development of CRC and its potential clinlic significance are reviewed in this article.
［Abstract］GCC/PKG/VASP，PKA/VASP et.al are associated with the occurrence and development of colorectal cancer. As one of the important signaling molecules of above signal pathways，VASP has constributed to the invasion，metastasis，apoptosis，angiogenesis and other events of colorectal cancer. The role of VASP signaling pathways in the development of CRC and its potential clinlic significance are reviewed in this article.
2016, 37(10)
Abstract:
［Abstract］Diabetic nephropathy（DN）is one of the severe chronic complications of diabetes, and has become an important contributing factor of end-stage renal disease and increased mortality of diabetes. DN pathogenesis is complicated，involving many factors working together. Regenerative medicine based on stem cells for the treatment of DN brings new hope. The mechanism of mesenchymal stem cells（MSCs）improving DN development get more and more attention，in this paper，we will talk about MSCs in improving DN through paracrine effect，hope it will provide the new mentality for the treatment of DN.
［Abstract］Diabetic nephropathy（DN）is one of the severe chronic complications of diabetes, and has become an important contributing factor of end-stage renal disease and increased mortality of diabetes. DN pathogenesis is complicated，involving many factors working together. Regenerative medicine based on stem cells for the treatment of DN brings new hope. The mechanism of mesenchymal stem cells（MSCs）improving DN development get more and more attention，in this paper，we will talk about MSCs in improving DN through paracrine effect，hope it will provide the new mentality for the treatment of DN.
2016, 37(10)
Abstract:
［Abstract］Objective To investigate the application of of Gaussian 09/GuassView 5.0 in spectra teaching in "Analytical Chemistry". Method Undergraduates of Pharmaceutical Science in 2014 grade of Kunming Medical University were selected to teach with a method with the help of Gaussian 09/GuassView 5.0 soft. Calculations of UV spectra，IR spectra and NMR spectra of compounds were introduced to make better understanding in the learning of relative knowledge points. The teaching effect was evaluated by the comparison of theoretical exam results of 2013 grade which didn't use soft．Result The theoretical test results showed that the scoring averages of the 2014 grade in UV， IR and NMR spectra were significantly higher than that in 2013 grade（P＜0.05）. Conclusion Gaussian 09/GuassView 5.0 can visualize the nonobjective knowledge， and imporve the students' interesting， thus improving the efficiency of teaching and learning.
［Abstract］Objective To investigate the application of of Gaussian 09/GuassView 5.0 in spectra teaching in "Analytical Chemistry". Method Undergraduates of Pharmaceutical Science in 2014 grade of Kunming Medical University were selected to teach with a method with the help of Gaussian 09/GuassView 5.0 soft. Calculations of UV spectra，IR spectra and NMR spectra of compounds were introduced to make better understanding in the learning of relative knowledge points. The teaching effect was evaluated by the comparison of theoretical exam results of 2013 grade which didn't use soft．Result The theoretical test results showed that the scoring averages of the 2014 grade in UV， IR and NMR spectra were significantly higher than that in 2013 grade（P＜0.05）. Conclusion Gaussian 09/GuassView 5.0 can visualize the nonobjective knowledge， and imporve the students' interesting， thus improving the efficiency of teaching and learning.
Application of the Integrated Teaching Method of PBL and TBL Based on Cases in Pharmacology Teaching
2016, 37(10)
Abstract:
［Abstract］Objective To explore the application of the integrated teaching method of PBL and TBL based on cases in pharmacology teaching. Method Ninety-two students majored in Pharmaceutical Preparations were selected into the control group，and 184 students majored in pharmacy were enrolled into the experimental group. The teaching effect was comprehensively evaluated by using the combination of theoretical examination and student feedback. Results In the experimental group，the average score of students in Pharmacology（73.68±9.40）was significantly higher than that of the control group（67.34±7.18），and the feedback of the students in the experimental group was significantly better than that in the control group. Conclusion Compared with the traditional teaching method， the integrated teaching method can significantly improve the students' theoretical examination results and students' learning interest.
［Abstract］Objective To explore the application of the integrated teaching method of PBL and TBL based on cases in pharmacology teaching. Method Ninety-two students majored in Pharmaceutical Preparations were selected into the control group，and 184 students majored in pharmacy were enrolled into the experimental group. The teaching effect was comprehensively evaluated by using the combination of theoretical examination and student feedback. Results In the experimental group，the average score of students in Pharmacology（73.68±9.40）was significantly higher than that of the control group（67.34±7.18），and the feedback of the students in the experimental group was significantly better than that in the control group. Conclusion Compared with the traditional teaching method， the integrated teaching method can significantly improve the students' theoretical examination results and students' learning interest.
2016, 37(10)
Abstract:
［Abstract］Objective To evaluate the effect of nursing intervention on the psychological status, complications and satisfaction in patients with cerebral hemorrhage. Methods A total of 184 patients with cerebral hemorrhage in the Third People's Hospital of Panzhihua City from January 2013 to June 2015 were randomly divided into observation group and control group with 92 cases in each group. The control group was treated with routine internal medicine nursing, and the observation group received nursing intervention on the basis of the control group. After 1 month, the Self-Rating Anxiety Scale（SAS）and Self-Rating Depression Scale（SDS）were used to evaluate the psychological status of the two groups before and after nursing. The incidence of complications and satisfaction were compared between the two groups. The quality of life of the two groups were assessed by the life activity ability rating（FIM） scale and the quality of life index （QLI）. Results Compared with the control group, the SAS and SDS score of the observation group after the intervention were significantly improved（P＜0.05）. The incidence of the observation group was significantly lower than that of the control group, and satisfaction was significantly higher than that of the control group（P＜0.05）. The FIM and QLI of the observation group were significantly better than that of the control group（P＜0.05）. Conclusion Nursing intervention can significantly improve the psychological status and quality of life, reduce the incidence of complications, and improve the satisfaction of patients with cerebral hemorrhage. It is worth popularizing widely.
［Abstract］Objective To evaluate the effect of nursing intervention on the psychological status, complications and satisfaction in patients with cerebral hemorrhage. Methods A total of 184 patients with cerebral hemorrhage in the Third People's Hospital of Panzhihua City from January 2013 to June 2015 were randomly divided into observation group and control group with 92 cases in each group. The control group was treated with routine internal medicine nursing, and the observation group received nursing intervention on the basis of the control group. After 1 month, the Self-Rating Anxiety Scale（SAS）and Self-Rating Depression Scale（SDS）were used to evaluate the psychological status of the two groups before and after nursing. The incidence of complications and satisfaction were compared between the two groups. The quality of life of the two groups were assessed by the life activity ability rating（FIM） scale and the quality of life index （QLI）. Results Compared with the control group, the SAS and SDS score of the observation group after the intervention were significantly improved（P＜0.05）. The incidence of the observation group was significantly lower than that of the control group, and satisfaction was significantly higher than that of the control group（P＜0.05）. The FIM and QLI of the observation group were significantly better than that of the control group（P＜0.05）. Conclusion Nursing intervention can significantly improve the psychological status and quality of life, reduce the incidence of complications, and improve the satisfaction of patients with cerebral hemorrhage. It is worth popularizing widely.
2016, 37(10)
Abstract:
［Abstract］Objective To investigate the application effect of indwelling needle puncture in the axillary vein of newborn infants. Methods From September 2015 to February 2016， the venous indwelling needle puncture was given for neonatal transfusion 180 cases， including 90 cases of the axillary vein indwelling needle infusion in the experimental group， and 90 cases of puncture of extremity vein infusion in the control group. The application effect of the 2 groups was compared. Results There were significant differences in terms of indwelling needle shedding， phlebitis，drug extravasation， lien time between the experimental group and the control group （P < 0.05） . Conclusions During neonatal indwelling needle puncture infusion process，the axillary vein indwelling needle infusion can prevent the indwelling needle shedding， decrease the incidence of phlebitis and reduce the incidence of drug extravasation，and can prolong the indwelling time. Puncture in the simple operation easy， is easy to learn and no need for special facilities and equipment，only conventional intravenous infusion required material，plus 24g indwelling needle，aseptic transparent dressing，paper tape，economical and practical，suitable for application in neonatal ward.
［Abstract］Objective To investigate the application effect of indwelling needle puncture in the axillary vein of newborn infants. Methods From September 2015 to February 2016， the venous indwelling needle puncture was given for neonatal transfusion 180 cases， including 90 cases of the axillary vein indwelling needle infusion in the experimental group， and 90 cases of puncture of extremity vein infusion in the control group. The application effect of the 2 groups was compared. Results There were significant differences in terms of indwelling needle shedding， phlebitis，drug extravasation， lien time between the experimental group and the control group （P < 0.05） . Conclusions During neonatal indwelling needle puncture infusion process，the axillary vein indwelling needle infusion can prevent the indwelling needle shedding， decrease the incidence of phlebitis and reduce the incidence of drug extravasation，and can prolong the indwelling time. Puncture in the simple operation easy， is easy to learn and no need for special facilities and equipment，only conventional intravenous infusion required material，plus 24g indwelling needle，aseptic transparent dressing，paper tape，economical and practical，suitable for application in neonatal ward.
2016, 37(10)
Abstract:
［Abstract］Objective To investigate the role of the extended-care in improving the compliance with low-salt diet in elderly patients with hypertension. Methods We selected 100 cases of elderly patients with hypertension in hospital.The 100 cases were divided into control group（50 cases）and observation group（50 cases） randomly.Observation group was given comprehensive intervention in low-salt diet education and control group was given traditional low-salt diet education.Tow groups were divided into three stages （on the day of admission，seven days after admission， one month after discharge）.We analyzed low-salt cognitive scores，low-salt behavioral scores and salt-intake/d comparably．Results On the day of admission，tow groups had no statistically significant difference（P＞0.05）in low-salt cognitive scores，low-salt behavioral scores and salt-intake/d；seven days after admission，tow groups had statistically significant difforence（P＜0.05）in low-salt cognitive scores，low-salt behavioral scores and salt-intake/d；one month after discharge，tow groups had statistically significant difference（P＜0.05）in low-salt cognitive scores，low-salt behavioral scores and salt-intake/d . Conclusion We use comprehensive intervention method to elderly patients with hypertension in low-salt diet education，through personalized low-salt diet education following evidence-based nursing，we can increase the compliance with low-salt diet in elderly patients with hypertension after discharge and improve blood pressure control effectively and to promote the image of the hospital and achieve the higher satisfaction from patients and families.
［Abstract］Objective To investigate the role of the extended-care in improving the compliance with low-salt diet in elderly patients with hypertension. Methods We selected 100 cases of elderly patients with hypertension in hospital.The 100 cases were divided into control group（50 cases）and observation group（50 cases） randomly.Observation group was given comprehensive intervention in low-salt diet education and control group was given traditional low-salt diet education.Tow groups were divided into three stages （on the day of admission，seven days after admission， one month after discharge）.We analyzed low-salt cognitive scores，low-salt behavioral scores and salt-intake/d comparably．Results On the day of admission，tow groups had no statistically significant difference（P＞0.05）in low-salt cognitive scores，low-salt behavioral scores and salt-intake/d；seven days after admission，tow groups had statistically significant difforence（P＜0.05）in low-salt cognitive scores，low-salt behavioral scores and salt-intake/d；one month after discharge，tow groups had statistically significant difference（P＜0.05）in low-salt cognitive scores，low-salt behavioral scores and salt-intake/d . Conclusion We use comprehensive intervention method to elderly patients with hypertension in low-salt diet education，through personalized low-salt diet education following evidence-based nursing，we can increase the compliance with low-salt diet in elderly patients with hypertension after discharge and improve blood pressure control effectively and to promote the image of the hospital and achieve the higher satisfaction from patients and families.
