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RSS2016 Vol. 37, No. 12
2016, 37(12): 1-7.
Abstract:
Objective To observe the expression profiles of miR-200b/c and their targets ZEB1/2 in early pulmonary fibrosis caused by acute lung injury induced by lipopolysaccharide in mice.Methods Early pulmonary fibrosis caused by acute lung injury is built via a lipopolysaccharide three-hit regimen.Mice were sacrificed at post-injective day 3, 7, 14, 21 respectively and the lung tissue specimens were collected.The lung tissue sections were stained with HE and Masson staining and pathological changes were observed by optical microscope.The expression profiles of miR-200 b, miR-200 c, ZEB1 m RNA and ZEB2 m RNA were detected by real-time PCR.Western blot was utilized to detect the levels of ZEB1,ZEB2,E-cadherin,Vimentin,α-SMA proteins.Results(1) pathological findings:compared with the control group,the collagen fibers deposited on on the third day after LPS treatment and pulmonary fibrosis gradually worsened;(2) Real time-PCR results:With theaggravation of pulmonary fibrosis,miR-200 b and miR-200 c levels were declined and the levels of miR-200b/c at post-injective day 7,14,21 were significantly lower than that of control group(P<0.01).ZEB1 m RNA and ZEB2 m RNA levels were gradually increased in the process of pulmonary fibrosis and the increased magnitude of ZEB2 m RNA was more significant than that of ZEB1 m RNA;(3) Western blot results:ZEB1 and ZEB2 protein levels were also gradually increased, consistent with their m RNA levels and the expression of E-cadherin protein was decreased while Vimentin and α-SMA protein levels increased with the evolution of pulmonary fibrosis caused by acute lung injury.Conclusion miR-200 b and miR-200 c promote epithelial-mesenchymal transition by inhibiting their targets ZEB1/2 in early pulmonary fibrosis caused by acute lung injury induced by LPS.
Objective To observe the expression profiles of miR-200b/c and their targets ZEB1/2 in early pulmonary fibrosis caused by acute lung injury induced by lipopolysaccharide in mice.Methods Early pulmonary fibrosis caused by acute lung injury is built via a lipopolysaccharide three-hit regimen.Mice were sacrificed at post-injective day 3, 7, 14, 21 respectively and the lung tissue specimens were collected.The lung tissue sections were stained with HE and Masson staining and pathological changes were observed by optical microscope.The expression profiles of miR-200 b, miR-200 c, ZEB1 m RNA and ZEB2 m RNA were detected by real-time PCR.Western blot was utilized to detect the levels of ZEB1,ZEB2,E-cadherin,Vimentin,α-SMA proteins.Results(1) pathological findings:compared with the control group,the collagen fibers deposited on on the third day after LPS treatment and pulmonary fibrosis gradually worsened;(2) Real time-PCR results:With theaggravation of pulmonary fibrosis,miR-200 b and miR-200 c levels were declined and the levels of miR-200b/c at post-injective day 7,14,21 were significantly lower than that of control group(P<0.01).ZEB1 m RNA and ZEB2 m RNA levels were gradually increased in the process of pulmonary fibrosis and the increased magnitude of ZEB2 m RNA was more significant than that of ZEB1 m RNA;(3) Western blot results:ZEB1 and ZEB2 protein levels were also gradually increased, consistent with their m RNA levels and the expression of E-cadherin protein was decreased while Vimentin and α-SMA protein levels increased with the evolution of pulmonary fibrosis caused by acute lung injury.Conclusion miR-200 b and miR-200 c promote epithelial-mesenchymal transition by inhibiting their targets ZEB1/2 in early pulmonary fibrosis caused by acute lung injury induced by LPS.
2016, 37(12): 8-11.
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Objective To study the growth difference and possible mechanism between nasopharyngeal carcinoma(NPC) cell line CNE-2 and its subclone S-18.Methods CNE-2 and S-18 cells were cultured in vitro.6×105 cells/mouse were xenografted subcutaneously in the back of nude mice.The volumes of tumors were measured on the 3 rd,7 th,10 th,14 th day after grafting.Mice were sacrificed on the 14 th day and tumors were isolated and weighed.RNA from tumor tissues were extracted and transcriptional levels of HSP27 and NF-ΚB were detected.Results(1) S-18, instead of CNE-2, grew to form tumor mass 7 days after xenografting subcutaneously; both cell lines formed tumor mass 10 days after xenografting, however, the volumes of S-18 tumors [(223.13±21.32) mm3, 10 th day;(420.25±24.52) mm3,14 th day] were significant bigger than CNE-2 tumors [(113.70±11.70) mm3,10 th day;(279.86±25.78) mm3,14 th day];The weights of S-18 tumors were significantly higher than CNE-2 tumors on the 14 th day after xenografting;(2) The transcriptional levels of HSP27 and NF-ΚB in S-18 tumor were significantly higher than in CNE-2 tumor.Conclusion Xenografted S-18 NPC grows faster than Xenografted CNE-2 NPC.HSP27 and NF-κB are probably involved in the regulation of growth in NPC.
Objective To study the growth difference and possible mechanism between nasopharyngeal carcinoma(NPC) cell line CNE-2 and its subclone S-18.Methods CNE-2 and S-18 cells were cultured in vitro.6×105 cells/mouse were xenografted subcutaneously in the back of nude mice.The volumes of tumors were measured on the 3 rd,7 th,10 th,14 th day after grafting.Mice were sacrificed on the 14 th day and tumors were isolated and weighed.RNA from tumor tissues were extracted and transcriptional levels of HSP27 and NF-ΚB were detected.Results(1) S-18, instead of CNE-2, grew to form tumor mass 7 days after xenografting subcutaneously; both cell lines formed tumor mass 10 days after xenografting, however, the volumes of S-18 tumors [(223.13±21.32) mm3, 10 th day;(420.25±24.52) mm3,14 th day] were significant bigger than CNE-2 tumors [(113.70±11.70) mm3,10 th day;(279.86±25.78) mm3,14 th day];The weights of S-18 tumors were significantly higher than CNE-2 tumors on the 14 th day after xenografting;(2) The transcriptional levels of HSP27 and NF-ΚB in S-18 tumor were significantly higher than in CNE-2 tumor.Conclusion Xenografted S-18 NPC grows faster than Xenografted CNE-2 NPC.HSP27 and NF-κB are probably involved in the regulation of growth in NPC.
2016, 37(12): 12-15.
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Objective To investigate the protective effect of fibrauretin injection against acute lung injury induced by lipopolysaccharide(LPS) in mice.Methods Seventy-two healthy male adult Kunming mice were randomly divided into 6 goups:normal control group,LPS group(intratracheal instilation of 5 mg/kg of LPS),hydrocortisone group(intraperitoneal injection of 3.3 mg/kg of hydrocortisone,once daily for 3 days,and intratracheal instilation of 5 mg/kg of LPS on the 4th day) and low dose,medium dose and high dose of fibrauretin groups(intraperitoneal injection of 2,10 and 50 mg/kg of fibrauretin,respectively,once daily for 3 days,and intratracheal instilation of 5 mg/kg of LPS on the 4th day).The mice in each group were sacrificed by dislocation 24 h after intratracheal instilation of LPS.The lung tissues of partial mice in each group were extracted and weighed to calculate the lung coefficients,and bronchoalveolar lavage(BAL) was performed in partial mice in each group to collectthe BALF for counting the inflammatory cells.Results The lung cofficients of mice in LPS group were significantly higher than those in the normal control group(P<0.05).The lung cofficients of mice in hydrocortisone group, low dose, medium dose and high dose of fibrauretin groups were significantly lower than those in LPS group(P <0.05).For the percentage of inflammatory cells in BALF and the percentage of neutrophils in inflammatory cells,LPS group was significantly higher than the normal control group(P<0.01),and compared with LPS group,hydrocortisone group,low dose,medium dose and high dose of fibrauretin groups were significantly decreased(P<0.01).Conclusion Fibrauretin injection can significantly ameliorate the inflammatory reaction degree in mice with acute lung injury induced by LPS.
Objective To investigate the protective effect of fibrauretin injection against acute lung injury induced by lipopolysaccharide(LPS) in mice.Methods Seventy-two healthy male adult Kunming mice were randomly divided into 6 goups:normal control group,LPS group(intratracheal instilation of 5 mg/kg of LPS),hydrocortisone group(intraperitoneal injection of 3.3 mg/kg of hydrocortisone,once daily for 3 days,and intratracheal instilation of 5 mg/kg of LPS on the 4th day) and low dose,medium dose and high dose of fibrauretin groups(intraperitoneal injection of 2,10 and 50 mg/kg of fibrauretin,respectively,once daily for 3 days,and intratracheal instilation of 5 mg/kg of LPS on the 4th day).The mice in each group were sacrificed by dislocation 24 h after intratracheal instilation of LPS.The lung tissues of partial mice in each group were extracted and weighed to calculate the lung coefficients,and bronchoalveolar lavage(BAL) was performed in partial mice in each group to collectthe BALF for counting the inflammatory cells.Results The lung cofficients of mice in LPS group were significantly higher than those in the normal control group(P<0.05).The lung cofficients of mice in hydrocortisone group, low dose, medium dose and high dose of fibrauretin groups were significantly lower than those in LPS group(P <0.05).For the percentage of inflammatory cells in BALF and the percentage of neutrophils in inflammatory cells,LPS group was significantly higher than the normal control group(P<0.01),and compared with LPS group,hydrocortisone group,low dose,medium dose and high dose of fibrauretin groups were significantly decreased(P<0.01).Conclusion Fibrauretin injection can significantly ameliorate the inflammatory reaction degree in mice with acute lung injury induced by LPS.
2016, 37(12): 16-19.
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Objective To evaluate the protective effects of Rosemary's chloroform extract on cerebral ischemia in mice and acute toxicity.Methods The protective of rosemary's chloroform extract on cerebral ischemia was observed and compared by the mice models of cerebral anoxia and ischemia,thrombus formation in vivo, and chloroform induced arrhythmias.Rosemary's Chloroform Extract was given orally to mice to evaluate acute toxicity.Results Rosemary's chloroform extract had different degrees of inhibition of collagen-the adrenaline induced thrombus formation,and prolonged acute ischemic mice brains off mouth breathing time and increase the number of mouth breathing(P <0.05 or P <0.01); Chloroform extract significantly reduced the incidence of chloroform induced ventricular arrhythmias.Acute toxicity results suggested that a female rat died in the next day of chloroform extract group,no additional toxicity was observed.Conclusion Rosemary's chloroform extract has significant protective effect on cerebral ischemia and hypoxia in mice.
Objective To evaluate the protective effects of Rosemary's chloroform extract on cerebral ischemia in mice and acute toxicity.Methods The protective of rosemary's chloroform extract on cerebral ischemia was observed and compared by the mice models of cerebral anoxia and ischemia,thrombus formation in vivo, and chloroform induced arrhythmias.Rosemary's Chloroform Extract was given orally to mice to evaluate acute toxicity.Results Rosemary's chloroform extract had different degrees of inhibition of collagen-the adrenaline induced thrombus formation,and prolonged acute ischemic mice brains off mouth breathing time and increase the number of mouth breathing(P <0.05 or P <0.01); Chloroform extract significantly reduced the incidence of chloroform induced ventricular arrhythmias.Acute toxicity results suggested that a female rat died in the next day of chloroform extract group,no additional toxicity was observed.Conclusion Rosemary's chloroform extract has significant protective effect on cerebral ischemia and hypoxia in mice.
2016, 37(12): 20-23.
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Objective To investigate the effects of uric acid(UA) and UA under oxidative stress on cultured human umbillical vein endothelial cells(HUVEC).Methods HUVECs were incubated with different concentration UA(0,4,8,16 mg/d L),H2O2(500 mmol/l) and UA+H2O2(500 mmol/l) for 24,48 and 72 hours.Then we observed the morphology of HUVECs and evaluated the proliferation of HUVECs by MTT assay.NO and ET-1 in supernatant medium was detected by ELISA.Results For the viability of HUVECs,there was no statistically significant difference between 4 mg/d L UA group and control group after incubation for 24,48 and 72hours(P>0.05) and between UA groups(8 mg/d L and 12 mg/d L) and control group after incubation for 24, 48 and72 hours(P>0.05).After incubation with 12 mg/d L of UA for 48 hours or 8 mg/d L of UA for 72 hours,the viability of HUVECs decreased significantly(P <0.05) The viability of HUVECs in H2O2 group decreased significantly(P<0.05).The viability of HUVECs in UA+H2O2groups after incubation for 24 h was significantly better than H2O2 group.There was no signifiant difference in the cell viability between(8 mg/d L or 12 mg/d L)UA+H2O2group and H2O2 group. Compared with the control group, the NO levels were decreased and the ET-1 levels were increased in the supernatants of HUVECs in 12 mg/d L UA group for 72 hours(P<0.05). Compared with H2O2 group,the NO levels were increased and the ET-1 levels were decreased in the supernatants of HUVECs in(8mg/d L or 12 mg/d L) UA +H2O2groups for 24 hours(P<0.05),while for(12 mg/d L) UA +H2O2group for 72 hours, the results were just the contary.Conclusion The effects of UA on HUVECs are related with both concentration and action time.Acutely increased UA may protect HUVECs form injury,while long action of UA may injure HUVECs,especially under oxidative stress.
Objective To investigate the effects of uric acid(UA) and UA under oxidative stress on cultured human umbillical vein endothelial cells(HUVEC).Methods HUVECs were incubated with different concentration UA(0,4,8,16 mg/d L),H2O2(500 mmol/l) and UA+H2O2(500 mmol/l) for 24,48 and 72 hours.Then we observed the morphology of HUVECs and evaluated the proliferation of HUVECs by MTT assay.NO and ET-1 in supernatant medium was detected by ELISA.Results For the viability of HUVECs,there was no statistically significant difference between 4 mg/d L UA group and control group after incubation for 24,48 and 72hours(P>0.05) and between UA groups(8 mg/d L and 12 mg/d L) and control group after incubation for 24, 48 and72 hours(P>0.05).After incubation with 12 mg/d L of UA for 48 hours or 8 mg/d L of UA for 72 hours,the viability of HUVECs decreased significantly(P <0.05) The viability of HUVECs in H2O2 group decreased significantly(P<0.05).The viability of HUVECs in UA+H2O2groups after incubation for 24 h was significantly better than H2O2 group.There was no signifiant difference in the cell viability between(8 mg/d L or 12 mg/d L)UA+H2O2group and H2O2 group. Compared with the control group, the NO levels were decreased and the ET-1 levels were increased in the supernatants of HUVECs in 12 mg/d L UA group for 72 hours(P<0.05). Compared with H2O2 group,the NO levels were increased and the ET-1 levels were decreased in the supernatants of HUVECs in(8mg/d L or 12 mg/d L) UA +H2O2groups for 24 hours(P<0.05),while for(12 mg/d L) UA +H2O2group for 72 hours, the results were just the contary.Conclusion The effects of UA on HUVECs are related with both concentration and action time.Acutely increased UA may protect HUVECs form injury,while long action of UA may injure HUVECs,especially under oxidative stress.
2016, 37(12): 24-28.
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Objective To learn the species distrilbution and drug-resistance of Gram-negative bacilli among bloodstream infections(BSI) in Yunnan province of China during 2012 to 2014.Methods Bloodstream infected Gram-negative bacilli were collected from 28 general hospitals in Yunan province of China.Data from all hospitals were applied with the same method of bacteria culture,iso1 ation,identification and antibiotic sensitivity tests.WHONET 5.6 was used to perform the statistical analysis.Results A total of 9042 clinical strains of non-repetitive gram-negative bacilli were collected.Enterobacteriaceae and Non-fermenter bacteria accounted for82.4% and 17.6% respectively.Enterobacteriaceae were mainly composed of Escherichia coli and Klebsiella pneumoniae.Non-fermenter bacteria were mainly composed of Acinetobacter baumannii and Pseudomonas aerouginosa.Escherichia coli accounted for the majority 49.29%, other species were Klebsiella pneumoniae13.17%, Acinetobacter baumannii 4.04% and Pseudomonas aeroginosa 3.85%.The susceptible rate of Enterobacteriaceae strains to the first generation cephalosporin was lower than 60%.The susceptible rate of E coliand Klebsiella pneumoniae to impenem was close to 100%, to Amikacin was more than 85%, to piperacillin-tazobactam was more than 70%, to Cefepime was more than 70%.But the susceptible rate of Klebsiella pneumoniae to impnem and amikacin decreased year by year from 2012 to 2014.The susceptible rate of Enterobacter cloacae to Amikacin was 86.4%~93.6%, Ciprofloxacin 70.5~76%,Cefepime 72.1~82.8%.It was less than other Enterobacteriaceae.The susceptible rate of Non-fermenter bacteria to normal antibiotics was much lower than Enterobacteriaceae.The susceptible rates of Pseudomonas aeroginosa to Impnem was 58.9%,Tobramycin 85%,Ciprofloxacin 71.7%,Amikacin 82.9%,Piperacillintazobactam 75.3%,Piperacillin59.6%,Atreonam 46.5%, Ceftazidime 69.1% and Cefepime 68.9% respectively.Furthermore, Acinetobacter baumannii's durg-resistance was more severe.The susceptible rate of Acinetobacter baumannii was lower than30%, to the third and fourth generation cephalosporin, the susceptible rate of Ampicillin/Sulbactam,Cefoperazone/Sulbactam, Carbapenems, Piperacillin/Tazuobatan, Quinolones and Carbapenems was less than40%.Conclusion Gram-negative bacilli have low susceptibilities among BSI.E.coli is the most common pathegon among BSI.The resistant rate of Non-fermenter bacteria to normal antibiotics is severe in hospitals.
Objective To learn the species distrilbution and drug-resistance of Gram-negative bacilli among bloodstream infections(BSI) in Yunnan province of China during 2012 to 2014.Methods Bloodstream infected Gram-negative bacilli were collected from 28 general hospitals in Yunan province of China.Data from all hospitals were applied with the same method of bacteria culture,iso1 ation,identification and antibiotic sensitivity tests.WHONET 5.6 was used to perform the statistical analysis.Results A total of 9042 clinical strains of non-repetitive gram-negative bacilli were collected.Enterobacteriaceae and Non-fermenter bacteria accounted for82.4% and 17.6% respectively.Enterobacteriaceae were mainly composed of Escherichia coli and Klebsiella pneumoniae.Non-fermenter bacteria were mainly composed of Acinetobacter baumannii and Pseudomonas aerouginosa.Escherichia coli accounted for the majority 49.29%, other species were Klebsiella pneumoniae13.17%, Acinetobacter baumannii 4.04% and Pseudomonas aeroginosa 3.85%.The susceptible rate of Enterobacteriaceae strains to the first generation cephalosporin was lower than 60%.The susceptible rate of E coliand Klebsiella pneumoniae to impenem was close to 100%, to Amikacin was more than 85%, to piperacillin-tazobactam was more than 70%, to Cefepime was more than 70%.But the susceptible rate of Klebsiella pneumoniae to impnem and amikacin decreased year by year from 2012 to 2014.The susceptible rate of Enterobacter cloacae to Amikacin was 86.4%~93.6%, Ciprofloxacin 70.5~76%,Cefepime 72.1~82.8%.It was less than other Enterobacteriaceae.The susceptible rate of Non-fermenter bacteria to normal antibiotics was much lower than Enterobacteriaceae.The susceptible rates of Pseudomonas aeroginosa to Impnem was 58.9%,Tobramycin 85%,Ciprofloxacin 71.7%,Amikacin 82.9%,Piperacillintazobactam 75.3%,Piperacillin59.6%,Atreonam 46.5%, Ceftazidime 69.1% and Cefepime 68.9% respectively.Furthermore, Acinetobacter baumannii's durg-resistance was more severe.The susceptible rate of Acinetobacter baumannii was lower than30%, to the third and fourth generation cephalosporin, the susceptible rate of Ampicillin/Sulbactam,Cefoperazone/Sulbactam, Carbapenems, Piperacillin/Tazuobatan, Quinolones and Carbapenems was less than40%.Conclusion Gram-negative bacilli have low susceptibilities among BSI.E.coli is the most common pathegon among BSI.The resistant rate of Non-fermenter bacteria to normal antibiotics is severe in hospitals.
2016, 37(12): 29-33.
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Objective To analyze the prevalence of diabetes and its impact on the quality of life(QOL)among Jingpo ethinic residents in Yunnan province.Methods Questionnaire survey and fasting blood glucose(FBG) testing were conducted among 1367 Jingpo residents aged≥35 years selected with random sampling method in Mangshi municipality of Dehong prefecture in Yunnan province.The Medical Outcome Study 36-Item Short-Form Health Survey(SF-36) was used in the survey.Results The prevalence rate of diabetes among the Jingpo residents was 4.1%,and 4.2% for males,4.0% for females.Among Jingpo diabetic patients,mean scores for the domains of physical functioning(PF),role physical(RP),bodily pain(BP),general health(GH),vitality(VT),social function(SF),role emotional(RE),mental health(MH),physical component summary(PCS) and mental component summary(MCS) were(84.64 ±16.81),(53.13 ±45.24),(71.70 ±24.28),(49.46±21.38),(65.09±11.02),(90.08 ±14.00),(79.17 ±39.98),(70.29 ±13.73),(57.12 ±9.39)and(55.07 ±6.58), respectively.The identified diabetic patients had significantly lower QOL scores for the doamins of PF,RP,BP and PCS than their counterparts,respectively(P<0.05).The results of the multiple linear regression analysis showed that older Jingpo residents had lower scores in the domain of PCS and MCS(P<0.01);men has lower scores in the domain of PCS and MCS than women(P<0.01);the higher the level of education,the higher the scores of PCS and MCS(P<0.01);married Jingpo residents had higher scores in domain of PCS than those of unmarried and divorced(P<0.01);the diabetic patients had lower scores of PCS than their counterparts(P<0.01).Conclusion It is necessary to strengthen the health education for the elderly and people with low level of education,and improve the management of patients with diabetes,so as to effectively improve the QOL of Jingpo ethnic minority residents.
Objective To analyze the prevalence of diabetes and its impact on the quality of life(QOL)among Jingpo ethinic residents in Yunnan province.Methods Questionnaire survey and fasting blood glucose(FBG) testing were conducted among 1367 Jingpo residents aged≥35 years selected with random sampling method in Mangshi municipality of Dehong prefecture in Yunnan province.The Medical Outcome Study 36-Item Short-Form Health Survey(SF-36) was used in the survey.Results The prevalence rate of diabetes among the Jingpo residents was 4.1%,and 4.2% for males,4.0% for females.Among Jingpo diabetic patients,mean scores for the domains of physical functioning(PF),role physical(RP),bodily pain(BP),general health(GH),vitality(VT),social function(SF),role emotional(RE),mental health(MH),physical component summary(PCS) and mental component summary(MCS) were(84.64 ±16.81),(53.13 ±45.24),(71.70 ±24.28),(49.46±21.38),(65.09±11.02),(90.08 ±14.00),(79.17 ±39.98),(70.29 ±13.73),(57.12 ±9.39)and(55.07 ±6.58), respectively.The identified diabetic patients had significantly lower QOL scores for the doamins of PF,RP,BP and PCS than their counterparts,respectively(P<0.05).The results of the multiple linear regression analysis showed that older Jingpo residents had lower scores in the domain of PCS and MCS(P<0.01);men has lower scores in the domain of PCS and MCS than women(P<0.01);the higher the level of education,the higher the scores of PCS and MCS(P<0.01);married Jingpo residents had higher scores in domain of PCS than those of unmarried and divorced(P<0.01);the diabetic patients had lower scores of PCS than their counterparts(P<0.01).Conclusion It is necessary to strengthen the health education for the elderly and people with low level of education,and improve the management of patients with diabetes,so as to effectively improve the QOL of Jingpo ethnic minority residents.
2016, 37(12): 34-36.
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Objective To learn the levels of serum 25-(OH)D in 0~12 years old chindren in Kunming.Methods Serum 25-(OH)D levels of children(4 498) were measured by using Roche cobas 8 000/E602 automatic electrochemical luminescence immunoassay.Results As the increase of age,the levels of serum 25-(OH)D were gradually decreased,different age groups had significant difference(P<0.05).The levels of 25-(OH)D between boys and girls had no significant difference(P>0.05);The average level of sernm 25-(OH)D was 83.48 nmol/L,the level of serum 25-(OH)D was distributed in 0~1 years old group,and most concentrated in the 6~9 years old group.Conclusion The levels of 25-(OH)D in 0~12 years old chindren are in a good status,but with the increase of age,the level of 25-(OH)D is decreasing gradually, especially the levels of 25-(OH)D decreased significantly after the age of three, in shortage or lack of status, this should cause everybody's attention.
Objective To learn the levels of serum 25-(OH)D in 0~12 years old chindren in Kunming.Methods Serum 25-(OH)D levels of children(4 498) were measured by using Roche cobas 8 000/E602 automatic electrochemical luminescence immunoassay.Results As the increase of age,the levels of serum 25-(OH)D were gradually decreased,different age groups had significant difference(P<0.05).The levels of 25-(OH)D between boys and girls had no significant difference(P>0.05);The average level of sernm 25-(OH)D was 83.48 nmol/L,the level of serum 25-(OH)D was distributed in 0~1 years old group,and most concentrated in the 6~9 years old group.Conclusion The levels of 25-(OH)D in 0~12 years old chindren are in a good status,but with the increase of age,the level of 25-(OH)D is decreasing gradually, especially the levels of 25-(OH)D decreased significantly after the age of three, in shortage or lack of status, this should cause everybody's attention.
2016, 37(12): 37-40.
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Objective To explore risk factors of unintentional injury among left-behind children in a rural poverty county and to provide evidence for injury intervention.Methods 2 909 students, including 1 630left-behind children,were recruited from a poor counties of five schools by Multi-stage stratified cluster random sampling method,and were surveyed applying designed questionnaire,interview method and observation method to collect the data of the injury accidents and related factors in the last one year.Results The incidence of unintentional injury among the left-behind children in rural area was 34.0%,higher than the rate of 27.7% among non-left-behind children and the difference was statistically significant(c2=13.541,P<0.05);in the top five injury type were falling injury(14.0%),burns(5.6%) and sharp instrument injury(3.6%),bite(3.0%),parts(1.6%) among left-behind children.Low age,often miss my mother,fighting in school,outdoor activities,road and traffic state were risk factors for injury of left-behind children.Conclusion The unintentional injury of left-behind children has become a social problem that can not be ignored, and targeted intervention should be carried out.
Objective To explore risk factors of unintentional injury among left-behind children in a rural poverty county and to provide evidence for injury intervention.Methods 2 909 students, including 1 630left-behind children,were recruited from a poor counties of five schools by Multi-stage stratified cluster random sampling method,and were surveyed applying designed questionnaire,interview method and observation method to collect the data of the injury accidents and related factors in the last one year.Results The incidence of unintentional injury among the left-behind children in rural area was 34.0%,higher than the rate of 27.7% among non-left-behind children and the difference was statistically significant(c2=13.541,P<0.05);in the top five injury type were falling injury(14.0%),burns(5.6%) and sharp instrument injury(3.6%),bite(3.0%),parts(1.6%) among left-behind children.Low age,often miss my mother,fighting in school,outdoor activities,road and traffic state were risk factors for injury of left-behind children.Conclusion The unintentional injury of left-behind children has become a social problem that can not be ignored, and targeted intervention should be carried out.
2016, 37(12): 41-46.
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Objective To explore the current status of sexual education and real needs of college freshmen in Kunming.Methods By applying random sampling,350 students from three universities in Kunming were investigated by self-administered questionnaire,and 310 valid questionnaires were recovered.Results 23.9% students confirmed that the schools do not provide courses related to sexual health,and 22.9% of the surveyed students claimed that they even did not heard any sexual education courses in their schools.The main sources of students to acquire information in terms of sexuality and sexual health are network television,video magazines,friends and classmates.Both male and female students are more acceptable to carry out sex education lectures.There were significant differences between male and female students in the form of obtaining sexual education,the content of sexual education and the demand of sexual education in three aspects(P<0.05).Girls tend to carry out sexual education in the form of major courses,but boys tend to the form of playing video or distributing books and periodicals(P<0.05).Girls tend to obtain the courses on sexually disease,AIDS,heterosexual intercourse,or love psychology,but boys tend to contraceptive knowledge and skills,or Sexual value orientation(P<0.05).Girls are more likely to acquire the knowledge of masturbation,sexual health,or sexual health,but boys prefer to the knowledge of sexual psychology,parasexuality,or homosexuality(P<0.05).Conclusion As a main body of educational institute for young people,all universities should provide training courses related to sexuality and sexual health at the early stage of higher education in order to equip college students with essential knowledge and skills to have safer and positive impact on their college studies.
Objective To explore the current status of sexual education and real needs of college freshmen in Kunming.Methods By applying random sampling,350 students from three universities in Kunming were investigated by self-administered questionnaire,and 310 valid questionnaires were recovered.Results 23.9% students confirmed that the schools do not provide courses related to sexual health,and 22.9% of the surveyed students claimed that they even did not heard any sexual education courses in their schools.The main sources of students to acquire information in terms of sexuality and sexual health are network television,video magazines,friends and classmates.Both male and female students are more acceptable to carry out sex education lectures.There were significant differences between male and female students in the form of obtaining sexual education,the content of sexual education and the demand of sexual education in three aspects(P<0.05).Girls tend to carry out sexual education in the form of major courses,but boys tend to the form of playing video or distributing books and periodicals(P<0.05).Girls tend to obtain the courses on sexually disease,AIDS,heterosexual intercourse,or love psychology,but boys tend to contraceptive knowledge and skills,or Sexual value orientation(P<0.05).Girls are more likely to acquire the knowledge of masturbation,sexual health,or sexual health,but boys prefer to the knowledge of sexual psychology,parasexuality,or homosexuality(P<0.05).Conclusion As a main body of educational institute for young people,all universities should provide training courses related to sexuality and sexual health at the early stage of higher education in order to equip college students with essential knowledge and skills to have safer and positive impact on their college studies.
2016, 37(12): 47-50.
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Objective To study and observe the effect of transnasal endoscopic surgery in the treatment of patients with fungal paranasal sinusitis.Methods A total of 82 cases of patients with fungal paranasal sinusitis were collected and divided into the control group(40 cases) and the observation group(40 cases) according to the principles of random, single blind and parallel control design, the patients in the control group received conventional surgical treatment,and the patients in the observation group received transnasal endoscopic surgical treatment,the two groups were observed and compared in terms of treatment efficiency,sinus cavity scores in the lesion range,average operation time,postoperative complication rate and patients' satisfaction degree.Results The effective rate in treatment group was 94.6%,significantly higher than that of control group(80.0%);the lesions sinus cavity score was 0 points in observation group,significantly lower than the control group(13.1±1.9),the average operation time was(45.4±10.9) min in the observation group,significantly shorter in the control group(76.3 ±10.5) min; the postoperative complication rate was 3.2% in the observation group, which was significantly lower than that of 25.6% in the control group(P<0.05).Conclusion The transnasal endoscopic surgery in the treatment of patients with fungal paranasal sinusitis can effectively improve the treatment efficiency,remove the mold lesions in the sinuses of patients, shorten the operation time and reduce the postoperative complication rate,and is conducive to promoting the rehabilitation of patients and improving the quality of life of patients,thus it is worth popularization and application.
Objective To study and observe the effect of transnasal endoscopic surgery in the treatment of patients with fungal paranasal sinusitis.Methods A total of 82 cases of patients with fungal paranasal sinusitis were collected and divided into the control group(40 cases) and the observation group(40 cases) according to the principles of random, single blind and parallel control design, the patients in the control group received conventional surgical treatment,and the patients in the observation group received transnasal endoscopic surgical treatment,the two groups were observed and compared in terms of treatment efficiency,sinus cavity scores in the lesion range,average operation time,postoperative complication rate and patients' satisfaction degree.Results The effective rate in treatment group was 94.6%,significantly higher than that of control group(80.0%);the lesions sinus cavity score was 0 points in observation group,significantly lower than the control group(13.1±1.9),the average operation time was(45.4±10.9) min in the observation group,significantly shorter in the control group(76.3 ±10.5) min; the postoperative complication rate was 3.2% in the observation group, which was significantly lower than that of 25.6% in the control group(P<0.05).Conclusion The transnasal endoscopic surgery in the treatment of patients with fungal paranasal sinusitis can effectively improve the treatment efficiency,remove the mold lesions in the sinuses of patients, shorten the operation time and reduce the postoperative complication rate,and is conducive to promoting the rehabilitation of patients and improving the quality of life of patients,thus it is worth popularization and application.
2016, 37(12): 51-56.
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Objective To compare the control effect on postoperative GER disease between tubular EC stomach esophagus anastomosis and the traditional full stomach esophagus anastomosis.Methods From September2010 to October 2015 in Bozhou People's Hospital, 85 patients diagnosed with esophageal cancer undergoing elective resection were randomly divided into a tubular stomach EC group(45 cases) and total gastrectomy group(40 cases), two patients underwent esophageal resection, wherein the tubular stomach set of rows of tubular esophagus stomach anastomosis, total gastrectomy group underwent conventional full stomach esophagus anastomosis.After the surgery until the patient to return to normal gastrointestinal function uses dynamic monitor its p H 24 h esophageal p H monitoring chamber,the other respectively after 1 March using RDQ Scale GER-relatedsymptoms in patients with score, at the same time Statistics after 1 March of the occurrence of GER.Results There were no deaths occurred,and no occurrence of postoperative anastomotic fistula and thoracic gastric emptying dysfunction,etc;the two groups were almost reached full monitoring 24 h, and between groups while monitoring the total time,Li position monitoring time,there was no significant supine monitoring time(P>0.05);24 h reflux episodes long tubular gastric reflux group and significantly less than the number of total gastrectomy group, the longest duration of reflux and p H value <4.00 The cumulative time was significantly shorter in total gastrectomy group,De Meester scores were significantly lower than the total gastrectomy group,between groups were statistically significant(P<0.01);postoperative gastric tube 1,March RDQ score and incidence of GER significantly lower than the total gastrectomy group, between groups were statistically significant(P<0.01 or P<0.05).Conclusion Tubular stomach esophagus anastomosis compared with conventional full stomach esophagus anastomosis resection of esophageal cancer has a more ideal GER disease control effect, and can provide a reference for the choice of nastomosis ways for patients with esophageal cancer surgery.
Objective To compare the control effect on postoperative GER disease between tubular EC stomach esophagus anastomosis and the traditional full stomach esophagus anastomosis.Methods From September2010 to October 2015 in Bozhou People's Hospital, 85 patients diagnosed with esophageal cancer undergoing elective resection were randomly divided into a tubular stomach EC group(45 cases) and total gastrectomy group(40 cases), two patients underwent esophageal resection, wherein the tubular stomach set of rows of tubular esophagus stomach anastomosis, total gastrectomy group underwent conventional full stomach esophagus anastomosis.After the surgery until the patient to return to normal gastrointestinal function uses dynamic monitor its p H 24 h esophageal p H monitoring chamber,the other respectively after 1 March using RDQ Scale GER-relatedsymptoms in patients with score, at the same time Statistics after 1 March of the occurrence of GER.Results There were no deaths occurred,and no occurrence of postoperative anastomotic fistula and thoracic gastric emptying dysfunction,etc;the two groups were almost reached full monitoring 24 h, and between groups while monitoring the total time,Li position monitoring time,there was no significant supine monitoring time(P>0.05);24 h reflux episodes long tubular gastric reflux group and significantly less than the number of total gastrectomy group, the longest duration of reflux and p H value <4.00 The cumulative time was significantly shorter in total gastrectomy group,De Meester scores were significantly lower than the total gastrectomy group,between groups were statistically significant(P<0.01);postoperative gastric tube 1,March RDQ score and incidence of GER significantly lower than the total gastrectomy group, between groups were statistically significant(P<0.01 or P<0.05).Conclusion Tubular stomach esophagus anastomosis compared with conventional full stomach esophagus anastomosis resection of esophageal cancer has a more ideal GER disease control effect, and can provide a reference for the choice of nastomosis ways for patients with esophageal cancer surgery.
2016, 37(12): 57-61.
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Objective To disscuss and analyze the clinical effects of whole body mild hypothermia on MODS caused by cerebral hemorrhage.Methods One hundred and eighty patients with MODS caused by cerebral hemorrhage during the period of hospitalized from January 2013 to December 2015 in ICU of our hospital were divided into two groups(observation group and control group) randomly,90 cases patients in each group.Patients in control group were treated with conventional treatment intervention,patients in control group were treated with whole body mild hypothermia on the base of conventional treatment intervention,the score of MODS,the ratio of,VO2 and DO2, the time during the period of hospitalized in ICU, the time of mechanical ventilation, epilepsy incidence and mortality, NIHSS score and PADL score of the patients in these two group after treatment were compared.Results After the comparion,the score of MODS and the state of oxygen consumption and oxygen supply of the patients in observation group were better than the patients in control group, there was significant differences,and had statistical significance(P<0.05);the time during the period of hospitalized in ICU and the time of mechanical ventilation of the patients in observation group were shorter than the patients in control group,there was significant difference,and had statistical significance(P<0.05);the mortality were reduce significantly of the patients in observation group than the patients in control group,there was significant difference,and had statistical significance(P<0.05);the epilepsy incidence of the patients in observation group and control has no significant difference,has no statistical significance(P>0.05);the NIHSS score and PADL score of the patients in observation group were higher than the patients in control group, there was significant difference, and has statistical significance(P<0.05).Conclusion The clinical effects of whole body mild hypothermia on MODS caused by cerebral hemorrhage have important significance,can effectively improve the balance of multiple organ dysfunction syndrome patients,can delay the progression of multiple organ dysfunction syndrome patients, improve the prognosis,it is worthy of clinical application and promotion.
Objective To disscuss and analyze the clinical effects of whole body mild hypothermia on MODS caused by cerebral hemorrhage.Methods One hundred and eighty patients with MODS caused by cerebral hemorrhage during the period of hospitalized from January 2013 to December 2015 in ICU of our hospital were divided into two groups(observation group and control group) randomly,90 cases patients in each group.Patients in control group were treated with conventional treatment intervention,patients in control group were treated with whole body mild hypothermia on the base of conventional treatment intervention,the score of MODS,the ratio of,VO2 and DO2, the time during the period of hospitalized in ICU, the time of mechanical ventilation, epilepsy incidence and mortality, NIHSS score and PADL score of the patients in these two group after treatment were compared.Results After the comparion,the score of MODS and the state of oxygen consumption and oxygen supply of the patients in observation group were better than the patients in control group, there was significant differences,and had statistical significance(P<0.05);the time during the period of hospitalized in ICU and the time of mechanical ventilation of the patients in observation group were shorter than the patients in control group,there was significant difference,and had statistical significance(P<0.05);the mortality were reduce significantly of the patients in observation group than the patients in control group,there was significant difference,and had statistical significance(P<0.05);the epilepsy incidence of the patients in observation group and control has no significant difference,has no statistical significance(P>0.05);the NIHSS score and PADL score of the patients in observation group were higher than the patients in control group, there was significant difference, and has statistical significance(P<0.05).Conclusion The clinical effects of whole body mild hypothermia on MODS caused by cerebral hemorrhage have important significance,can effectively improve the balance of multiple organ dysfunction syndrome patients,can delay the progression of multiple organ dysfunction syndrome patients, improve the prognosis,it is worthy of clinical application and promotion.
2016, 37(12): 62-66.
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Objective To analyze the CT characteristics of chronic schistosomiasis liver disease,in order to improve the accuracy of CT diagnosis for the disease.Methods We analyzed the CT features of 125 cases of clinical pathology diagnosis of chronic schistosomiasis liver and of 50 normal control group,and measured the hepatic lobe lines and spleen index.Results(1) Schistosoma calcification:In 125 cases,there were 120 patients with varying degrees of liver calcification, in which 76 cases of intrahepatic or subcapsularlinear calcification,44 cases of reticular or map-like calcification,33 cases of calcification portal system,15 cases of spleen calcification,85 cases of intestinal wall calcification;(2) Morphological changes of the liver and spleen:The transverse diameter of the left hepatic lobe,caudate lobe,and caudate lobe-right lobe ratio were larger in patients with chronic schistosomiasis than controls,the transverse diameter of the right hepatic lobe were smaller and there were statistically difference(P<0.001).There were 82 cases of expanded gallbladder fossa in chronic schistosomiasis.Splenic index in patients with chronic schistosomiasis and had no obvious difference in the control group(P>0.05);(3) Schistosomiasis liver's complications:there were 43 cases of cholecystitis and cholelithiasis,11 cases of liver cancer,5 cases of colon cancer,3 cases of bladder cancer.Conclusion Intrahepatic calcification and the left hepatic lobe and caudate lobe enlargement are CT signs of chronic schistosomiasis,which is often merged with many complications.
Objective To analyze the CT characteristics of chronic schistosomiasis liver disease,in order to improve the accuracy of CT diagnosis for the disease.Methods We analyzed the CT features of 125 cases of clinical pathology diagnosis of chronic schistosomiasis liver and of 50 normal control group,and measured the hepatic lobe lines and spleen index.Results(1) Schistosoma calcification:In 125 cases,there were 120 patients with varying degrees of liver calcification, in which 76 cases of intrahepatic or subcapsularlinear calcification,44 cases of reticular or map-like calcification,33 cases of calcification portal system,15 cases of spleen calcification,85 cases of intestinal wall calcification;(2) Morphological changes of the liver and spleen:The transverse diameter of the left hepatic lobe,caudate lobe,and caudate lobe-right lobe ratio were larger in patients with chronic schistosomiasis than controls,the transverse diameter of the right hepatic lobe were smaller and there were statistically difference(P<0.001).There were 82 cases of expanded gallbladder fossa in chronic schistosomiasis.Splenic index in patients with chronic schistosomiasis and had no obvious difference in the control group(P>0.05);(3) Schistosomiasis liver's complications:there were 43 cases of cholecystitis and cholelithiasis,11 cases of liver cancer,5 cases of colon cancer,3 cases of bladder cancer.Conclusion Intrahepatic calcification and the left hepatic lobe and caudate lobe enlargement are CT signs of chronic schistosomiasis,which is often merged with many complications.
2016, 37(12): 67-71.
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Objective To explore the value of DWI(diffusion weighted imaging,DWI) in diagnosis of bladder cancer in clinical T stage by comparing DWI and CT.Methods Seventy-five patients with bladder cancer underwent MRI plain scan,enhanced diffusion-weighted imaging and CT scan,to make a diagnosis preoperative clinical T stage according to their image.Then we compared the diffusion-weighted imaging and CT results with pathological staging results to assess the value of DWI in bladder tumor staging.Results For clinical T staging bladder cancer,the diagnostic accuracy rate was 83.5% and 72% for DWI and CT,resectively,statistical analysis showed a significant difference(P <0.05).Conclusion Magnetic resonance diffusion weighted imaging can accurately diagose the clinical T stage of bladder cancer,and has a higher value than CT.
Objective To explore the value of DWI(diffusion weighted imaging,DWI) in diagnosis of bladder cancer in clinical T stage by comparing DWI and CT.Methods Seventy-five patients with bladder cancer underwent MRI plain scan,enhanced diffusion-weighted imaging and CT scan,to make a diagnosis preoperative clinical T stage according to their image.Then we compared the diffusion-weighted imaging and CT results with pathological staging results to assess the value of DWI in bladder tumor staging.Results For clinical T staging bladder cancer,the diagnostic accuracy rate was 83.5% and 72% for DWI and CT,resectively,statistical analysis showed a significant difference(P <0.05).Conclusion Magnetic resonance diffusion weighted imaging can accurately diagose the clinical T stage of bladder cancer,and has a higher value than CT.
2016, 37(12): 72-75.
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Objective To explore the application efficacy of scalp nerve block combined with sevoflurane in craniotomy.Methods Fifty-five patients undergoing craniotomy in pingdingshan second people's hospital of henan province from Mar 2013 to Mar 2015 were randomly divided into observation group(n=28) and control group(n =27).The scalp nerve block combined with sevoflurane was performed in observation group and the sevoflurane intravenous inhalational combined with sufentanil was performed in control group.The changes of MAP and HR were observed before induction,5min after induction,skin incision,break the meninges,operation end and 5min after catheter removal.The agitation scale after operation,anesthesia duration,awaking time and dosage of sufentanil were compared.Results MAP and HR at skin incision,break the meninges,operation end and5 min after catheter removal in control group were significantly increased than those before operation, and significantly higher than those in observation group at same stage of operation, with statistically significancedifferences(P <0.05).The agitation scale of observation group was lower than that of control group, with statistically significance difference(P <0.05).There was no significance difference between two groups in anesthesia duration(P>0.05),the awaking time in observation group was significantly shorter than that in control group and the dosage of sufentanil was significantly reduced in observation group(P<0.05).Conclusion Scalp nerve block combined with sevoflurane used in craniotomy has advantages in stable vital signs,rapid awaking,less dosage of sufentanil and lower score of agitation,which is suitable for clinical application.
Objective To explore the application efficacy of scalp nerve block combined with sevoflurane in craniotomy.Methods Fifty-five patients undergoing craniotomy in pingdingshan second people's hospital of henan province from Mar 2013 to Mar 2015 were randomly divided into observation group(n=28) and control group(n =27).The scalp nerve block combined with sevoflurane was performed in observation group and the sevoflurane intravenous inhalational combined with sufentanil was performed in control group.The changes of MAP and HR were observed before induction,5min after induction,skin incision,break the meninges,operation end and 5min after catheter removal.The agitation scale after operation,anesthesia duration,awaking time and dosage of sufentanil were compared.Results MAP and HR at skin incision,break the meninges,operation end and5 min after catheter removal in control group were significantly increased than those before operation, and significantly higher than those in observation group at same stage of operation, with statistically significancedifferences(P <0.05).The agitation scale of observation group was lower than that of control group, with statistically significance difference(P <0.05).There was no significance difference between two groups in anesthesia duration(P>0.05),the awaking time in observation group was significantly shorter than that in control group and the dosage of sufentanil was significantly reduced in observation group(P<0.05).Conclusion Scalp nerve block combined with sevoflurane used in craniotomy has advantages in stable vital signs,rapid awaking,less dosage of sufentanil and lower score of agitation,which is suitable for clinical application.
2016, 37(12): 76-81.
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Objectives To study the role of IL-17 and IL-23 in non-Hodgkin lymphoma(NHL) by detecting the expression levels of IL-17 and IL-23 in peripheral blood of B-cell non-Hodgkin lymphoma patients treated by RCHOP.Methods T wenty-five patients with B-cell NHL who achieved remission after 6 to 8 cycles of R-CHOP as a NHL group,20 healthy volunteers were recruited as a normal control group.RT-PCR was used to detect the expression levels of IL-17 m RNA and IL-23 m RNA in NHL patients and health volunteers.Results The expression level of IL-17 m RNA and IL-23 m RNAin the patients with NHL before therapy and the patients with NHL who achieved remission was lower than that in the normal control group(P<0.05).The expression level of IL-17 m RNA and IL-23 m RNA in the patients with NHL who achieved remission was higher than that in the patients with NHL before therapy(P=0.001,P<0.05).Conclusion IL-17 and IL-23 m RNA expressions are higher after treated with R-CHOP.The expression levels of IL-17 m RNA and IL-23 m RNA in NHL patients are related with prognosis and efficacy.
Objectives To study the role of IL-17 and IL-23 in non-Hodgkin lymphoma(NHL) by detecting the expression levels of IL-17 and IL-23 in peripheral blood of B-cell non-Hodgkin lymphoma patients treated by RCHOP.Methods T wenty-five patients with B-cell NHL who achieved remission after 6 to 8 cycles of R-CHOP as a NHL group,20 healthy volunteers were recruited as a normal control group.RT-PCR was used to detect the expression levels of IL-17 m RNA and IL-23 m RNA in NHL patients and health volunteers.Results The expression level of IL-17 m RNA and IL-23 m RNAin the patients with NHL before therapy and the patients with NHL who achieved remission was lower than that in the normal control group(P<0.05).The expression level of IL-17 m RNA and IL-23 m RNA in the patients with NHL who achieved remission was higher than that in the patients with NHL before therapy(P=0.001,P<0.05).Conclusion IL-17 and IL-23 m RNA expressions are higher after treated with R-CHOP.The expression levels of IL-17 m RNA and IL-23 m RNA in NHL patients are related with prognosis and efficacy.
2016, 37(12): 82-84.
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Objective To investigate the effect of probiotics on the blood lipid metabolism of elderly patients with coronary artery disease(CHD).Methods The clinical data of 100 elderly CHD patients who were admitted in our hospital from May 2012 ~November 2015 were collected.The enrolled patients were randomly divided into observation group and control group, 50 cases in each group.All patients were routinely given medication for secondary prevention of CHD,patients in the observation group were additionally given oral probiotics.Then we observed the changes in the lipid levels of patients before and after 12 weeks treatment.Results The serum total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C) of patients in the observation group were improved more significantly than the control group,the difference was significant(P<0.05).The serum total High-density lipoprotein cholesterol(HDL-C) was improved more significantly than the control group.Conclusion Probiotics adjuvant therapy can significantly improve the blood lipid indexes in elderly CHD patients.
Objective To investigate the effect of probiotics on the blood lipid metabolism of elderly patients with coronary artery disease(CHD).Methods The clinical data of 100 elderly CHD patients who were admitted in our hospital from May 2012 ~November 2015 were collected.The enrolled patients were randomly divided into observation group and control group, 50 cases in each group.All patients were routinely given medication for secondary prevention of CHD,patients in the observation group were additionally given oral probiotics.Then we observed the changes in the lipid levels of patients before and after 12 weeks treatment.Results The serum total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C) of patients in the observation group were improved more significantly than the control group,the difference was significant(P<0.05).The serum total High-density lipoprotein cholesterol(HDL-C) was improved more significantly than the control group.Conclusion Probiotics adjuvant therapy can significantly improve the blood lipid indexes in elderly CHD patients.
2016, 37(12): 85-88.
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Objective To evaluate the influence of tracheal intubation guided with light wand on hemodynamics in patients with obstructive sleep apnea syndrome.Methods Seventy-four patients with obstructive sleep apnea syndrome were randomly divided into light wand group(n=37) and laryngoscope group(n=37).The time to successful intubation, rate of successful intubation, changes of heart rate(HR), systolic blood pressure(SBP),diastolic blood pressure(DBP) and complications were compared in two groups.Results The time to successful intubation of light wand group was significantly shorter than that of laryngoscope group,while the rate of successful intubation in light wand group was significantly higher than that in laryngoscope group(P<0.05).The HR,SBP and DBP at T1 were significantly lower than those at T0 in two groups(P<0.05).The HR,SBP and DBP in two groups at T2,T3 were significantly higher than those at T1(P<0.05),and those indexes of light wand group were significantly lower than laryngoscope group at T2 and T3(P<0.05).The incidence rates of complications in light wand group were significantly lower than those in laryngoscope group(P<0.05).Conclusion Withthe advantage of higher success rate,shorter time to successful intubation,more stable hemodynamics and fewer complications,tracheal intubation guided with light wand is worthy of popularization and application in clinical.
Objective To evaluate the influence of tracheal intubation guided with light wand on hemodynamics in patients with obstructive sleep apnea syndrome.Methods Seventy-four patients with obstructive sleep apnea syndrome were randomly divided into light wand group(n=37) and laryngoscope group(n=37).The time to successful intubation, rate of successful intubation, changes of heart rate(HR), systolic blood pressure(SBP),diastolic blood pressure(DBP) and complications were compared in two groups.Results The time to successful intubation of light wand group was significantly shorter than that of laryngoscope group,while the rate of successful intubation in light wand group was significantly higher than that in laryngoscope group(P<0.05).The HR,SBP and DBP at T1 were significantly lower than those at T0 in two groups(P<0.05).The HR,SBP and DBP in two groups at T2,T3 were significantly higher than those at T1(P<0.05),and those indexes of light wand group were significantly lower than laryngoscope group at T2 and T3(P<0.05).The incidence rates of complications in light wand group were significantly lower than those in laryngoscope group(P<0.05).Conclusion Withthe advantage of higher success rate,shorter time to successful intubation,more stable hemodynamics and fewer complications,tracheal intubation guided with light wand is worthy of popularization and application in clinical.
2016, 37(12): 89-93.
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Objective To study the feasibility and safety of coronary sinus(CS) ventricular pacing comparing with traditional right ventricular(RV) pacing in ordinary pacing treatment.Methods Sixty-one patients with an indication of pacemaker implantation were randomized into traditional RV pacing group and CS ventricular pacing group. The success rate,complications,preoperative and postoperative QRS wave duration and pacemaker parameters were compared between the two groups.Results(1) The instant success rate in traditional RV pacing and the CS pacing groups were 100% and 68.97%(P<0.01); the surgical success rate was significantly higher by using left ventricular electrode than the ventricle tined electrode in CS pacing group(85.71%and 25%,P<0.05). There was no significant difference in the incidence of complications between the two groups(P>0.05);(2) The increase of QRS complex width at postoperative 1 month in CS pacing group is significantly less than the RV pacing group(P<0.05);(3) Although the pacing threshold and electrode impedance during operation,postoperative 1 month and 3 months in CS pacing group were higher than RV pacing group(P<0.05),the CS pacing was effective.Conclusion The CS ventricular pacing is as safe and effective as traditional RVpacing,and is more consistent with physiological ventricular activation sequence.Using left ventricular electrode can significantly improve the success rate of CS ventricular pacing.
Objective To study the feasibility and safety of coronary sinus(CS) ventricular pacing comparing with traditional right ventricular(RV) pacing in ordinary pacing treatment.Methods Sixty-one patients with an indication of pacemaker implantation were randomized into traditional RV pacing group and CS ventricular pacing group. The success rate,complications,preoperative and postoperative QRS wave duration and pacemaker parameters were compared between the two groups.Results(1) The instant success rate in traditional RV pacing and the CS pacing groups were 100% and 68.97%(P<0.01); the surgical success rate was significantly higher by using left ventricular electrode than the ventricle tined electrode in CS pacing group(85.71%and 25%,P<0.05). There was no significant difference in the incidence of complications between the two groups(P>0.05);(2) The increase of QRS complex width at postoperative 1 month in CS pacing group is significantly less than the RV pacing group(P<0.05);(3) Although the pacing threshold and electrode impedance during operation,postoperative 1 month and 3 months in CS pacing group were higher than RV pacing group(P<0.05),the CS pacing was effective.Conclusion The CS ventricular pacing is as safe and effective as traditional RVpacing,and is more consistent with physiological ventricular activation sequence.Using left ventricular electrode can significantly improve the success rate of CS ventricular pacing.
2016, 37(12): 94-98.
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Objective To compare the clinical features of space infections in the oral maxillofacial region between patients with diabetes mellitus and patients without diabetes.Methods A retrospective study was conducted on 61 patients diagnosed with space infections in the oral maxillofacial region and receiving treatment at the department of oral and The 4nd Affiliated Hospital of Kunming Medical University between 2013 and 2016.The patients were divided into 2 groups:diabetic group(n =28) and non-diabetic group(n =33).The study variables were demography,etiology,clinical and laboratory examinations(bacteriology, admission blood glucose level,admission white blood cell count and percent of neutrophil on admission).The outcome was compared.The length of hospital stay and complications were also compared.Statistical analyses of the results between groups were performed using the t test and chi square test.Results For oral and maxillofacial space infection,there was no statistically significant difference in gender between the diabetic group and(28 cases) and non diabetes group(33cases)(χ2test,P=0.5929);while there were statistically significant differences between two groups in age(t test,P<0.001) and in the number of infection space(t test,P<0.001);there was no significant difference between the two groups in the sources of infection(χ2,P>0.05);The main source of infection was odontogenic,followed by adenogenous.There were statistically significant differences between two groups in blood glucose concentration at admission(t test,P<0.001) and in the hospitalization time and complications(t test and χ2test P<0.05),while no statistically significant differences in the white blood cells count and neutrophil count(t test,P >0.05).Compared with the non diabetic group,for patients in the diabetic group,the age of onset was older,the involvd gaps were more,the blood glucose concentration was higher on admission,the length of stay was longer,complications were more likely to occur,and the consequences were more serious.Conclusion Compared with non diabetes group,space infection in oral and maxillofacial region is more serious and the hospital stay is longer and has more complications in diabetic patients which should be paid higher attention to.
Objective To compare the clinical features of space infections in the oral maxillofacial region between patients with diabetes mellitus and patients without diabetes.Methods A retrospective study was conducted on 61 patients diagnosed with space infections in the oral maxillofacial region and receiving treatment at the department of oral and The 4nd Affiliated Hospital of Kunming Medical University between 2013 and 2016.The patients were divided into 2 groups:diabetic group(n =28) and non-diabetic group(n =33).The study variables were demography,etiology,clinical and laboratory examinations(bacteriology, admission blood glucose level,admission white blood cell count and percent of neutrophil on admission).The outcome was compared.The length of hospital stay and complications were also compared.Statistical analyses of the results between groups were performed using the t test and chi square test.Results For oral and maxillofacial space infection,there was no statistically significant difference in gender between the diabetic group and(28 cases) and non diabetes group(33cases)(χ2test,P=0.5929);while there were statistically significant differences between two groups in age(t test,P<0.001) and in the number of infection space(t test,P<0.001);there was no significant difference between the two groups in the sources of infection(χ2,P>0.05);The main source of infection was odontogenic,followed by adenogenous.There were statistically significant differences between two groups in blood glucose concentration at admission(t test,P<0.001) and in the hospitalization time and complications(t test and χ2test P<0.05),while no statistically significant differences in the white blood cells count and neutrophil count(t test,P >0.05).Compared with the non diabetic group,for patients in the diabetic group,the age of onset was older,the involvd gaps were more,the blood glucose concentration was higher on admission,the length of stay was longer,complications were more likely to occur,and the consequences were more serious.Conclusion Compared with non diabetes group,space infection in oral and maxillofacial region is more serious and the hospital stay is longer and has more complications in diabetic patients which should be paid higher attention to.
2016, 37(12): 99-102.
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Objective To investigate the etiology, clinical characteristics, diagnosis and treatment of female uterine empyema.Methods We retrospectively analyzed the clinical data of 31 patients with uterine empyema in our hospital from January 2003 to December 2015.Results Uterine empyema was caused as a result of occlusion of the cervical canal,Patients had no typical symptoms,four cases had no symptoms(12.90%),twenty-two cases had abdominal pain(70.97%),twenty-seven cases had vaginal discharge(98.09%) and twelve cases had sanguinopurulent material(38.71%),three cases of them had fever(9.70%).Gynecological ultrasound examination was an important diagnostic tool.Dilatation of cervix, discharging purulent material, antibiotics treatment,the cervical adhesions were manually broken down to establish a patent cervical lumen to accommodate a uterine Fleischl catheter,the uterus was lavaged with 0.9% Nacl solution, metronidazole,gentamicin.The above treatment got a good therapeutic effect.Conclusion Pyometra has atypical symptoms with difficult diagnosis,and develops gradually.Gynecological ultrasound examination has great clinical significance.Full dilatation of cervix,discharging purulent material are the key to the success of conservation treatment and can achieve good results.Combination of endometrial cancer or other malignant tumor patients should take surgery as soon as possible,according to the tolerance ability.
Objective To investigate the etiology, clinical characteristics, diagnosis and treatment of female uterine empyema.Methods We retrospectively analyzed the clinical data of 31 patients with uterine empyema in our hospital from January 2003 to December 2015.Results Uterine empyema was caused as a result of occlusion of the cervical canal,Patients had no typical symptoms,four cases had no symptoms(12.90%),twenty-two cases had abdominal pain(70.97%),twenty-seven cases had vaginal discharge(98.09%) and twelve cases had sanguinopurulent material(38.71%),three cases of them had fever(9.70%).Gynecological ultrasound examination was an important diagnostic tool.Dilatation of cervix, discharging purulent material, antibiotics treatment,the cervical adhesions were manually broken down to establish a patent cervical lumen to accommodate a uterine Fleischl catheter,the uterus was lavaged with 0.9% Nacl solution, metronidazole,gentamicin.The above treatment got a good therapeutic effect.Conclusion Pyometra has atypical symptoms with difficult diagnosis,and develops gradually.Gynecological ultrasound examination has great clinical significance.Full dilatation of cervix,discharging purulent material are the key to the success of conservation treatment and can achieve good results.Combination of endometrial cancer or other malignant tumor patients should take surgery as soon as possible,according to the tolerance ability.
2016, 37(12): 103-106.
Abstract:
Objective To compare the effect on intraoperative cerebral metabolism between the propofol combined with remifentanil infusion in total intravenous anesthesia and the desflurane combined with remifentanil intravenous inhalation anesthesia in patients in neurosurgery.Methods Thirty-four patients were randomly divided into the propofol combined with remifentanil group(group A,n=16) and the desflurane combined withremifentanil group(group B,n=18).The B-ultrasound under the guide of retrograde catheterization through right internal jugular vein and artery was prepared after the patients entered the operation room.Atropine,propofol,fentanyl, rocuronium were used in the induction of anesthesia.The mechanical ventilation was applied after conventional trachea cannula.Once patients were anesthetized steadily, jugular bulb venous oxygen saturation(Sjv O),jugular2 bulb venous oxygen partial pressure(Pjv O),Jugular2 Bulb venous hemoglogin(Hbv),jugular bulb venous lactate(Ljv),jugular bulb venous gluxose(Gv),arterial oxygen saturation(Sa O),arterial2 oxygen pressure(Pa O2),arterin(Hba),arterial blood lactate(La),arterial blood gluxose(Ga),arterial blood oxygen content(Ca O2), jugular bulb venous oxygen content(Cjv O2), arteriovenous oxygen difference(AVDO2),cerebral extraction of oxygen(CEO2),cerebral lactate production rate(CLP) and cerebral glucose uptake rate(CGU) at different time [before anesthesia induction(T1),1 hours after the start of the operation(T2),2 hours after the start of the operation(T3),half an hour after the operation]were collected.Results(1) The value of Sjv O2,Pjv O2,Cjv O2 and Ca O2 in group B was significantly higher than that in group A(P<0.001);The value of AVDO2 and CEO2in group B was lower than that in group A(P<0.05);(2) The value of Gv and Ga in group B was higher than that in group A(P<0.05);There were no significant differences about CGU in two groups(P>0.05);(3) The value of CLP in group B was lower than that in group A(P<0.05);there were no significant differences about Ljv and La in two groups(P>0.05);(4) Compared with the value at moment of T1 between group A and group B,the value of Pa O2,Sa O2,Pjv O2 and Sjv O2 were increased with time(P<0.05),the value of Ca O2, Cjv O2, AVDO2 and CEO2showed a downward trend(P <0.05).Conclusion(1) Both total intravenous anesthesia and intravenous inhalation anesthesia can reduce the cerebral oxygen metabolism;(2) For the cerebral protection of neurosurgery operation,it seems that the effect of intravenous inhalation anesthesia is more stronger than total intravenous anesthesia.
Objective To compare the effect on intraoperative cerebral metabolism between the propofol combined with remifentanil infusion in total intravenous anesthesia and the desflurane combined with remifentanil intravenous inhalation anesthesia in patients in neurosurgery.Methods Thirty-four patients were randomly divided into the propofol combined with remifentanil group(group A,n=16) and the desflurane combined withremifentanil group(group B,n=18).The B-ultrasound under the guide of retrograde catheterization through right internal jugular vein and artery was prepared after the patients entered the operation room.Atropine,propofol,fentanyl, rocuronium were used in the induction of anesthesia.The mechanical ventilation was applied after conventional trachea cannula.Once patients were anesthetized steadily, jugular bulb venous oxygen saturation(Sjv O),jugular2 bulb venous oxygen partial pressure(Pjv O),Jugular2 Bulb venous hemoglogin(Hbv),jugular bulb venous lactate(Ljv),jugular bulb venous gluxose(Gv),arterial oxygen saturation(Sa O),arterial2 oxygen pressure(Pa O2),arterin(Hba),arterial blood lactate(La),arterial blood gluxose(Ga),arterial blood oxygen content(Ca O2), jugular bulb venous oxygen content(Cjv O2), arteriovenous oxygen difference(AVDO2),cerebral extraction of oxygen(CEO2),cerebral lactate production rate(CLP) and cerebral glucose uptake rate(CGU) at different time [before anesthesia induction(T1),1 hours after the start of the operation(T2),2 hours after the start of the operation(T3),half an hour after the operation]were collected.Results(1) The value of Sjv O2,Pjv O2,Cjv O2 and Ca O2 in group B was significantly higher than that in group A(P<0.001);The value of AVDO2 and CEO2in group B was lower than that in group A(P<0.05);(2) The value of Gv and Ga in group B was higher than that in group A(P<0.05);There were no significant differences about CGU in two groups(P>0.05);(3) The value of CLP in group B was lower than that in group A(P<0.05);there were no significant differences about Ljv and La in two groups(P>0.05);(4) Compared with the value at moment of T1 between group A and group B,the value of Pa O2,Sa O2,Pjv O2 and Sjv O2 were increased with time(P<0.05),the value of Ca O2, Cjv O2, AVDO2 and CEO2showed a downward trend(P <0.05).Conclusion(1) Both total intravenous anesthesia and intravenous inhalation anesthesia can reduce the cerebral oxygen metabolism;(2) For the cerebral protection of neurosurgery operation,it seems that the effect of intravenous inhalation anesthesia is more stronger than total intravenous anesthesia.
2016, 37(12): 107-110.
Abstract:
Objective To discuss the application of Mini Mental State Scale(the mini-mental state examination MMSE) test in assess the effect of dezocine analgesia therapy on the cognitive function of patients with agitation in recovery period of general anesthesia after gynecological laparoscopic surgery.Methods We observed210 cases of elective gynecologic laparoscopic surgery in our hospital from January 2015 to December 2015,ASA grade I or II.All patients were transrered to PACU(ICU) after extubation, and were divided into three groups:A,B,C group,each with 70 cases in each group.Patients with severe agitation in A group were timely given 10 mg of dezocine, patients with mild agitation in B group were given 10 mg of dezocine after a delay of 5~10 minutes,and patients without agitation in C group were not given dezocine.Then the MMSE test was performed to evaluate the cognition scores of patients in the three groups.The MMSE scores immediately, 15 minutes, 30 minutes, 60 minutes after given dezocine and 24 hours after operation were recorded.Results The statistical results showed that there were significant differences in the MMSE scores of patients immediately and at 30 minutes after given dezocine between three groups(P<0.05);and there was no significant difference in the MMSE scores of patients at60 minutes after given dezocine and 24 hours after operation between three groups(P >0.05).Conclusion Dezocine analgesia therapy has no effect on the cognitive function of patients with agitation in recovery period of general anesthesia after gynecological laparoscopic surgery.Dezocine can be safely used for analgesia and sedationtreatment for patients with agitation in recovery period of general anesthesia after gynecological laparoscopic surgery analgesia,sedation agitation.
Objective To discuss the application of Mini Mental State Scale(the mini-mental state examination MMSE) test in assess the effect of dezocine analgesia therapy on the cognitive function of patients with agitation in recovery period of general anesthesia after gynecological laparoscopic surgery.Methods We observed210 cases of elective gynecologic laparoscopic surgery in our hospital from January 2015 to December 2015,ASA grade I or II.All patients were transrered to PACU(ICU) after extubation, and were divided into three groups:A,B,C group,each with 70 cases in each group.Patients with severe agitation in A group were timely given 10 mg of dezocine, patients with mild agitation in B group were given 10 mg of dezocine after a delay of 5~10 minutes,and patients without agitation in C group were not given dezocine.Then the MMSE test was performed to evaluate the cognition scores of patients in the three groups.The MMSE scores immediately, 15 minutes, 30 minutes, 60 minutes after given dezocine and 24 hours after operation were recorded.Results The statistical results showed that there were significant differences in the MMSE scores of patients immediately and at 30 minutes after given dezocine between three groups(P<0.05);and there was no significant difference in the MMSE scores of patients at60 minutes after given dezocine and 24 hours after operation between three groups(P >0.05).Conclusion Dezocine analgesia therapy has no effect on the cognitive function of patients with agitation in recovery period of general anesthesia after gynecological laparoscopic surgery.Dezocine can be safely used for analgesia and sedationtreatment for patients with agitation in recovery period of general anesthesia after gynecological laparoscopic surgery analgesia,sedation agitation.
2016, 37(12): 111-115.
Abstract:
Objective To investigate the clinical characteristics of peptic ulcer in young and middle-aged patients and old-aged patients,and to improve the awareness of clinicians.Methods A retrospective analysis was performed in 351 cases(old-aged group) and 351 cases(young and middle-aged group) of peptic ulcer in our hospital from Jan,2012 to Jan,2013.The clinical data were compared between the two groups,including clinical symptoms, complications, ulcer site, size, concomitant diseases, treatment and outcome.Results(1)Clinical symptoms:the symptoms were not typical in old-aged group compared to young and middle-aged group(80.3% vs 73%),complications as the initial symptom was more common(46.7% vs 29.6%,P<0.001), typical upper abdominal pain were lower than those in the young and middle-aged group(19.7% vs 37%,P<0.001);(2) Common complications:old-aged patients with complications was significantly higher than in the young and middle-aged group(46.7% vs 29.6%,P<0.05),in which bleeding,perforation and obstruction were common;(3) Ulcer site:gastric ulcer,elevation ulcer werw more common in old-aged patients than young and middle-aged patients(P<0.05);(4) Ulcer size:giant ulcer(more than 2.0 cm) was more common in old-aged patientsthan young and middle-aged patients(15.1% vs 4.3%,P<0.05);but there was no difference in the infection rate of Helicobacter pylori(HP) between them(90.3% vs 90.9%, P >0.05);(5) Concomitant diseases:the incidence of concomitant diseases was higher in old-aged patients, including diabetes, cardiovascular disease,gallstones, cholecystitis, gout(47% vs 15.7%, P <0.05);(6) Treatment and outcome:the mortality of old-aged patients with complications was significantly higher than those in the young and middle-aged group(14%vs 3.8%,P<0.05).Conclusion The clinical manifestations of peptic ulcer in old-aged patients are atypical,with characteristics of high morbidity, high incidence of complications, high mortality.Early diagnosis and treatment is essential to improve the prognosis and reduce mortality.
Objective To investigate the clinical characteristics of peptic ulcer in young and middle-aged patients and old-aged patients,and to improve the awareness of clinicians.Methods A retrospective analysis was performed in 351 cases(old-aged group) and 351 cases(young and middle-aged group) of peptic ulcer in our hospital from Jan,2012 to Jan,2013.The clinical data were compared between the two groups,including clinical symptoms, complications, ulcer site, size, concomitant diseases, treatment and outcome.Results(1)Clinical symptoms:the symptoms were not typical in old-aged group compared to young and middle-aged group(80.3% vs 73%),complications as the initial symptom was more common(46.7% vs 29.6%,P<0.001), typical upper abdominal pain were lower than those in the young and middle-aged group(19.7% vs 37%,P<0.001);(2) Common complications:old-aged patients with complications was significantly higher than in the young and middle-aged group(46.7% vs 29.6%,P<0.05),in which bleeding,perforation and obstruction were common;(3) Ulcer site:gastric ulcer,elevation ulcer werw more common in old-aged patients than young and middle-aged patients(P<0.05);(4) Ulcer size:giant ulcer(more than 2.0 cm) was more common in old-aged patientsthan young and middle-aged patients(15.1% vs 4.3%,P<0.05);but there was no difference in the infection rate of Helicobacter pylori(HP) between them(90.3% vs 90.9%, P >0.05);(5) Concomitant diseases:the incidence of concomitant diseases was higher in old-aged patients, including diabetes, cardiovascular disease,gallstones, cholecystitis, gout(47% vs 15.7%, P <0.05);(6) Treatment and outcome:the mortality of old-aged patients with complications was significantly higher than those in the young and middle-aged group(14%vs 3.8%,P<0.05).Conclusion The clinical manifestations of peptic ulcer in old-aged patients are atypical,with characteristics of high morbidity, high incidence of complications, high mortality.Early diagnosis and treatment is essential to improve the prognosis and reduce mortality.
2016, 37(12): 116-121.
Abstract:
Objective To explore the difference in knowledge, attitude and behavior of AIDS among adolescent users with new-type of drug between Chinese and Burmese in detoxification center of the Prefecture of Dehong and to provide basis for effective health education activities.Methods A cluster sampling method was used to interview adolescent users with a questionnaire survey in detoxification center of the Prefecture of Dehong.Results A total of 102 people were investigated,50 people were from China and 52 from Myanmar.There was a statistical difference in the survey of AIDS knowledge at the age of 18~25 years old(P<0.05),Chinese scores were higher.Their attitude in which“how much risk of HIV infection about yourself”had a statistical difference(P<0.05).There were significant differences in commercial sexual behavior between Chinese and Burmese(P<0.05).After the use of new drugs,there was 1 case of group sexual behavior occurred for Burmese,the frequency of condom use after drug use was not different from that of the regular sexual partner(P>0.05).At the non-regular partner the difference between the two groups was statistically significant(P <0.05).Conclusion AIDS knowledge,attitudes and behaviors are different in adolescent users with new-type of drug between Chinese andBurmese.The detoxification centers should carry out different health education activities according to the different knowledge,attitudes and behaviors of the two nationalities.
Objective To explore the difference in knowledge, attitude and behavior of AIDS among adolescent users with new-type of drug between Chinese and Burmese in detoxification center of the Prefecture of Dehong and to provide basis for effective health education activities.Methods A cluster sampling method was used to interview adolescent users with a questionnaire survey in detoxification center of the Prefecture of Dehong.Results A total of 102 people were investigated,50 people were from China and 52 from Myanmar.There was a statistical difference in the survey of AIDS knowledge at the age of 18~25 years old(P<0.05),Chinese scores were higher.Their attitude in which“how much risk of HIV infection about yourself”had a statistical difference(P<0.05).There were significant differences in commercial sexual behavior between Chinese and Burmese(P<0.05).After the use of new drugs,there was 1 case of group sexual behavior occurred for Burmese,the frequency of condom use after drug use was not different from that of the regular sexual partner(P>0.05).At the non-regular partner the difference between the two groups was statistically significant(P <0.05).Conclusion AIDS knowledge,attitudes and behaviors are different in adolescent users with new-type of drug between Chinese andBurmese.The detoxification centers should carry out different health education activities according to the different knowledge,attitudes and behaviors of the two nationalities.
2016, 37(12): 122-126.
Abstract:
Objective To learn the epidemiological characteristics of people with HIV infection newly found in the hospital.Methods We assessed the information of people with HIV infection in China information system for disease control and prevention which the superior agency corrected the information during January 1,2013 to December 31, 2015 in the hospital.Results Among the enrolled patients with HIV infection, male patients accounted for 82.11%,patients with education background less than primary school accounted for 44.74%,other county in the city accounted for 53.68%,unmarried sex communication accounted for 79.47%.Patients found by blood or preoperative examination accounted for 33.68%,patients married who has a spouse accounted for 57.89%,etc.old patients accounted for 27.37%.There was no statistically significant difference in gender, place of residence,type of medical treatment insurance among the empty nest old,etc.There was no statistically significant differences in gender(χ2=1.073,P=1.073),different ownership(χ2=2.618,P=0.622),different agegroups(χ2=4.375,P = 4.375),the different professional(χ2=0.868,P=0.868),different marital status(χ2=190,P =0.535),different cultural level(χ2=3.076,P=3.076),different transmission(χ2=1.614,P=1.614),different sample source(χ2=9.815,P=9.815).There was a statistically significant difference in suffering from venereal disease during different years, etc.Conclusion There is no change in demographic characteristics in new found people with HIV during 2013 to 2015.It is necessary to enhance HIV education in the crowd on the knowledge of HIV/AIDS prevention and control,especially for the people with unmarried sex contact and MSM same-sex sexual behaviors.We should provide some education on prevention and control HIV/AIDS to the HIV infected patients married with spouses, and given them the early HIV screening.The government should inprove the medical care and psychological counseling to improve the quality of life of elderly people with HIV infection.
Objective To learn the epidemiological characteristics of people with HIV infection newly found in the hospital.Methods We assessed the information of people with HIV infection in China information system for disease control and prevention which the superior agency corrected the information during January 1,2013 to December 31, 2015 in the hospital.Results Among the enrolled patients with HIV infection, male patients accounted for 82.11%,patients with education background less than primary school accounted for 44.74%,other county in the city accounted for 53.68%,unmarried sex communication accounted for 79.47%.Patients found by blood or preoperative examination accounted for 33.68%,patients married who has a spouse accounted for 57.89%,etc.old patients accounted for 27.37%.There was no statistically significant difference in gender, place of residence,type of medical treatment insurance among the empty nest old,etc.There was no statistically significant differences in gender(χ2=1.073,P=1.073),different ownership(χ2=2.618,P=0.622),different agegroups(χ2=4.375,P = 4.375),the different professional(χ2=0.868,P=0.868),different marital status(χ2=190,P =0.535),different cultural level(χ2=3.076,P=3.076),different transmission(χ2=1.614,P=1.614),different sample source(χ2=9.815,P=9.815).There was a statistically significant difference in suffering from venereal disease during different years, etc.Conclusion There is no change in demographic characteristics in new found people with HIV during 2013 to 2015.It is necessary to enhance HIV education in the crowd on the knowledge of HIV/AIDS prevention and control,especially for the people with unmarried sex contact and MSM same-sex sexual behaviors.We should provide some education on prevention and control HIV/AIDS to the HIV infected patients married with spouses, and given them the early HIV screening.The government should inprove the medical care and psychological counseling to improve the quality of life of elderly people with HIV infection.
2016, 37(12): 127-130.
Abstract:
MI is often associated with cardiac arrhythmias,and ventricular arrhythmia is the most common.MRI is a powerful tool for the assessment of myocardial viability,DE-MRI detection of myocardial scar is the“gold standard”for diagnosis myocardial infarction,Now we summarized the detection of myocardial infarction scar by DE-MRI and the relationship between myocardial infarction scar and ventricular arrhythmia.
MI is often associated with cardiac arrhythmias,and ventricular arrhythmia is the most common.MRI is a powerful tool for the assessment of myocardial viability,DE-MRI detection of myocardial scar is the“gold standard”for diagnosis myocardial infarction,Now we summarized the detection of myocardial infarction scar by DE-MRI and the relationship between myocardial infarction scar and ventricular arrhythmia.
2016, 37(12): 131-134.
Abstract:
Radiotherapy is one of the most important treatment method for head and neck cancer.The intensity-modulated radiotherapy(IMRT) has better conformal property and uniformity.IMRT is gradually replacing conventional radiation therapy and has become the mainstream radical treatment for head and neck cancer.During IMRT,the patient treatment positioning,inter-treatment,and intra-treatment variation of organ position,size,and shape impact the accuracy of radiation dose delivery.This method may cause target less and(or) additional complications.Adaptive radiotherapy(ART) is a new radiotherapy technology.Based on the patient's pre-treatment images and relative information such as dose deviation,ART can able to compensate the target coverage and clinical outcome.Under the help of ART technique,radiotherapy can be more accurate and more personalized.This paper reviews the research status of ART technology in head and neck cancerby retrospective studying the related literature at home and abroad.
Radiotherapy is one of the most important treatment method for head and neck cancer.The intensity-modulated radiotherapy(IMRT) has better conformal property and uniformity.IMRT is gradually replacing conventional radiation therapy and has become the mainstream radical treatment for head and neck cancer.During IMRT,the patient treatment positioning,inter-treatment,and intra-treatment variation of organ position,size,and shape impact the accuracy of radiation dose delivery.This method may cause target less and(or) additional complications.Adaptive radiotherapy(ART) is a new radiotherapy technology.Based on the patient's pre-treatment images and relative information such as dose deviation,ART can able to compensate the target coverage and clinical outcome.Under the help of ART technique,radiotherapy can be more accurate and more personalized.This paper reviews the research status of ART technology in head and neck cancerby retrospective studying the related literature at home and abroad.
The Research and Application Effects of Systematic Anatomy Learning System Based on Android Platform
2016, 37(12): 135-139.
Abstract:
Objective To implement a systematic anatomy learning system based on android platform and evaluate the application effects of the learning system in clinical medical undergraduate students.Methods The systematic anatomy learning system was based on android development package and correlation functions were implemented using java technique.108 undergraduate students in clinical major of 2014 in Kunming Medical University were selected and randomly divided into experimental group and control group.Students of the experimental group were given the traditional teaching method and this learning system for autonomous learning, while students of the control group were given the traditional teaching method.These two teaching methods were subjectively and objectively evaluated by questionnaire survey and final examination respectively.Results The questionnaire showed that the 87.5% of students thought that it was necessary to use the learning system.The90.74% of students in experimental group thought their learning effect were improved and the 98.15% of them thought themselves learning ability were improved.The average score of the experimental group and the control group was83.56±5.61 and 75.33±4.62 respectively.The difference between the two groups was statistically significant(P<0.05).Conclusion The systematic anatomy learning system can help students to understand and grasp the knowledge points of the systematic anatomy,and it can improve the teaching effect too.It will provide research foundation for the next step of applying for patent.
Objective To implement a systematic anatomy learning system based on android platform and evaluate the application effects of the learning system in clinical medical undergraduate students.Methods The systematic anatomy learning system was based on android development package and correlation functions were implemented using java technique.108 undergraduate students in clinical major of 2014 in Kunming Medical University were selected and randomly divided into experimental group and control group.Students of the experimental group were given the traditional teaching method and this learning system for autonomous learning, while students of the control group were given the traditional teaching method.These two teaching methods were subjectively and objectively evaluated by questionnaire survey and final examination respectively.Results The questionnaire showed that the 87.5% of students thought that it was necessary to use the learning system.The90.74% of students in experimental group thought their learning effect were improved and the 98.15% of them thought themselves learning ability were improved.The average score of the experimental group and the control group was83.56±5.61 and 75.33±4.62 respectively.The difference between the two groups was statistically significant(P<0.05).Conclusion The systematic anatomy learning system can help students to understand and grasp the knowledge points of the systematic anatomy,and it can improve the teaching effect too.It will provide research foundation for the next step of applying for patent.
2016, 37(12): 140-143.
Abstract:
Objective To describe the characteristics of organizational culture,to identify the level of quality of work life,and to explore the relationship between each dimension of organizational culture and quality of work life among nurses in tertiary hospitals in Yunnan Province,China.Methods The subjects were 416 nurses from six tertiary hospitals in Yunnan Province.Research instruments were the Organizational Culture Profile(OCP) and the Quality of Work Life Scale(QWLS).Results The results of this study showed that four dimensions of organizational culture including competitiveness(3.56 ±0.49); performance orientation(4.08 ±0.22); stability(3.68±0.43) and social responsibility(3.95±0.37) were prominent.The other 3 dimensions,namely supportiveness(3.19±0.79);innovation(3.21±0.62) and emphasis on rewards were perceived(2.42±0.79) as less prominent.The overall mean score of quality of work life was at a moderate level(57.46±15.17).Conclusion There are positive significant correlation between each dimension of organizational culture and quality of work life(P<0.01).Improvement of organizational culture can enhance quality of work life among nurses,and then guarantee the nursing quality.
Objective To describe the characteristics of organizational culture,to identify the level of quality of work life,and to explore the relationship between each dimension of organizational culture and quality of work life among nurses in tertiary hospitals in Yunnan Province,China.Methods The subjects were 416 nurses from six tertiary hospitals in Yunnan Province.Research instruments were the Organizational Culture Profile(OCP) and the Quality of Work Life Scale(QWLS).Results The results of this study showed that four dimensions of organizational culture including competitiveness(3.56 ±0.49); performance orientation(4.08 ±0.22); stability(3.68±0.43) and social responsibility(3.95±0.37) were prominent.The other 3 dimensions,namely supportiveness(3.19±0.79);innovation(3.21±0.62) and emphasis on rewards were perceived(2.42±0.79) as less prominent.The overall mean score of quality of work life was at a moderate level(57.46±15.17).Conclusion There are positive significant correlation between each dimension of organizational culture and quality of work life(P<0.01).Improvement of organizational culture can enhance quality of work life among nurses,and then guarantee the nursing quality.
2016, 37(12): 144-147.
Abstract:
Objective To observe the influence of optimized nursing process on the clinical effect and quality of life of patients with cerebral infarction received intravenous thrombolysis.Methods Sixty-two cerebral infarction patients with thrombolytic therapy in Panzhihua Central Hospital were treated as control group from January 2013 to June 2014,74 cases cerebral infarction patients with thrombolytic therapy as the observation group from July 2014 to October 2015.The control group received routine nursing, the observation group were given optimized nursing process on the basis of routine nursing.The clinical effect and quality of life were compared between the two groups.Results The triage time,referral time and hospital stay time of the observation group were significantly shorter than the control group, the differences were statistically significant(P <0.05).The effective rate in the control group was 70.97%,that of the observation group was 87.84%,the effective rate in observation group was significantly better than that of the control group,the difference was statistically significant(P <0.05).Compared with before treatment, the life quality of two groups were significantly improved after treatment,the difference was statistically significant(P<0.05);after treatment, the quality of life in observationgroups were better than that of the control groups,the difference is statistically significant(P<0.05).Conclusion Nursing process optimization can effectively improve the therapeutic effect for the patients with cerebral infarction intravenous thrombolytic therapy,shorten the treatment time and hospitalization time,and can significantly improve the patient's quality of life.It is valuable for clinical application.
Objective To observe the influence of optimized nursing process on the clinical effect and quality of life of patients with cerebral infarction received intravenous thrombolysis.Methods Sixty-two cerebral infarction patients with thrombolytic therapy in Panzhihua Central Hospital were treated as control group from January 2013 to June 2014,74 cases cerebral infarction patients with thrombolytic therapy as the observation group from July 2014 to October 2015.The control group received routine nursing, the observation group were given optimized nursing process on the basis of routine nursing.The clinical effect and quality of life were compared between the two groups.Results The triage time,referral time and hospital stay time of the observation group were significantly shorter than the control group, the differences were statistically significant(P <0.05).The effective rate in the control group was 70.97%,that of the observation group was 87.84%,the effective rate in observation group was significantly better than that of the control group,the difference was statistically significant(P <0.05).Compared with before treatment, the life quality of two groups were significantly improved after treatment,the difference was statistically significant(P<0.05);after treatment, the quality of life in observationgroups were better than that of the control groups,the difference is statistically significant(P<0.05).Conclusion Nursing process optimization can effectively improve the therapeutic effect for the patients with cerebral infarction intravenous thrombolytic therapy,shorten the treatment time and hospitalization time,and can significantly improve the patient's quality of life.It is valuable for clinical application.
2016, 37(12): 148-150.
Abstract:
Objective To study the clinical effect and significance of integrative medicines of TCM and WM combined with fast-track surgery concept on perioperative nursing in colorectal surgery.Methods One hundred and twenty patients with surgery were randomly divided into the observation group and the control group,60 cases in each group.The control group was treated with conventional nursing,while the observation group was treated with fast track surgery nursing of integrative medicines of TCM and WM.Results Bowel sound recovery time,anus exhaust time, defecation time, length of hospital stay and postoperative complications of observation group was significantly less than the control group(P<0.05).Nursing total satisfaction of observation group was significantly higher than the control group(P <0.05).Conclusion Integrative medicines of TCM and WM combined with fast-track surgery concept on perioperative nursing in colorectal surgery can reduce stress,speed up the recovery,reduce complications.So it is a safe and effective way of perioperative nursing,and worthy of clinical popularization and application.
Objective To study the clinical effect and significance of integrative medicines of TCM and WM combined with fast-track surgery concept on perioperative nursing in colorectal surgery.Methods One hundred and twenty patients with surgery were randomly divided into the observation group and the control group,60 cases in each group.The control group was treated with conventional nursing,while the observation group was treated with fast track surgery nursing of integrative medicines of TCM and WM.Results Bowel sound recovery time,anus exhaust time, defecation time, length of hospital stay and postoperative complications of observation group was significantly less than the control group(P<0.05).Nursing total satisfaction of observation group was significantly higher than the control group(P <0.05).Conclusion Integrative medicines of TCM and WM combined with fast-track surgery concept on perioperative nursing in colorectal surgery can reduce stress,speed up the recovery,reduce complications.So it is a safe and effective way of perioperative nursing,and worthy of clinical popularization and application.
