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RSS2017 Vol. 38, No. 06
2017, 38(06): 1-4.
Abstract:
Objective To construct a recombinant vector containing human WDR5 and establish LNCaP cell strain with stable expression of WDR5. Me thods WDR5 gene was amplified by PCR and was cloned into eukaryotic expression vector pCI-neo. The resulted pCI-neo-WDR5 plasmid was verified by restriction enzyme digestion and DNA sequencing. LNCaP cells were transfected with pCI-neo-WDR5 by liposome. The expression of WDR5 in the cells was examined by Real-time PCR. Re s ults Restriction enzyme digestion and sequencing analysis showed that WDR5 c DNA was correctly cloned into pCI-neo vector. After transfection of LNCaP cells with the recombinant plasmids, the monoclonal cell strain of stably expressed were obtained by G418 screening, which showed expressions of WDR5 m RNAs detected by Real Time-PCR. Conclus ion The recombinant of PCI-neo-WDR5 stably express in the transfected LNCaP cells, which laid a foundation for further study on WDR5.
Objective To construct a recombinant vector containing human WDR5 and establish LNCaP cell strain with stable expression of WDR5. Me thods WDR5 gene was amplified by PCR and was cloned into eukaryotic expression vector pCI-neo. The resulted pCI-neo-WDR5 plasmid was verified by restriction enzyme digestion and DNA sequencing. LNCaP cells were transfected with pCI-neo-WDR5 by liposome. The expression of WDR5 in the cells was examined by Real-time PCR. Re s ults Restriction enzyme digestion and sequencing analysis showed that WDR5 c DNA was correctly cloned into pCI-neo vector. After transfection of LNCaP cells with the recombinant plasmids, the monoclonal cell strain of stably expressed were obtained by G418 screening, which showed expressions of WDR5 m RNAs detected by Real Time-PCR. Conclus ion The recombinant of PCI-neo-WDR5 stably express in the transfected LNCaP cells, which laid a foundation for further study on WDR5.
2017, 38(06): 5-8.
Abstract:
目的 构建神经生长因子 (nerve growth factor, NGF) 基因-骨髓基质干细胞 (bone marrow stromal stem cells, BMSCs) 重组体, 将其移植入阿尔茨海默病 (alzheimer's disease, AD) 大鼠海马, 探讨AD大鼠神经行为学变化.方法 将Aβ淀粉蛋白 (β-amyloid protein) 立体定向注射入大鼠海马制备AD模型.动物随机分为5组:正常组 (Normal group) 、盐水注射组 (NS group) 、模型组 (Experiment group) 、BMSC移植组 (AD+BMSC group) 和NGF-BMSC移植组 (NGF-BMSC group) .移植2周后用Morris水迷宫检测大鼠行为学变化以评价移植效果.结果 移植BMSC和NGF-BMSC后, AD大鼠神经学学习、记忆能力比模型组显著提高, 而以后者效果更为显著 (分别为P<0.05和P<0.01) .结论 NGF基因修饰的BMSC可作为种子细胞传送NGF, 对大鼠AD的神经行为学起到显著的改善作用.
2017, 38(06): 9-14.
Abstract:
Objective To investigate the influence of bone marrow stem cell transplantation on immunosuppressed mice induced by radiotherapy. Me thods Female BALB/C mice were randomly divided into three groups. The mice in test group and in control group were irradiated with X-ray at dosage of 8Gy respectively, while those in blank group were not irradiated. After that, bone marrow stem cells 1x107 in 0.2ml normal saline were injected into mice by tail veins in test group, while 0.2ml normal saline (NS) was injected into mice by tail vein in control group. The peripheral blood was subjected to routine blood test on days 15, 25, 35 after transplantation, T lymphocytes (CD3~+, CD4~+, CD8~+) were determined by flow cytometry (FCM) on days15, 25, 35 after transplantation, and the concentration of mice peripheral blood immunoglobulins Ig M were measured by the ELISA method. All the mice were observed for survivals for 35 days. Re s ults The survival rates of mice 35 d after Hematopoietic stem cell transplantation were 80%, while 10% in control group. The leukocyte counts and Lymph% as well as CD3~+% in peripheral blood after transplantation increased as compared with those received NS (P <0.05) . However, CD4~+%, CD8~+% and serum immunoglobulin Ig M after transplantation decreased, which decreased remarkably on day 15 and begun to recover after that day. BMSC decreased Mono% and CD4~+/CD8~+in peripheral blood (P <0.05) .Conclus ion Bone marrow stem cell transplantation can repair the suppressed immunity of mice.
Objective To investigate the influence of bone marrow stem cell transplantation on immunosuppressed mice induced by radiotherapy. Me thods Female BALB/C mice were randomly divided into three groups. The mice in test group and in control group were irradiated with X-ray at dosage of 8Gy respectively, while those in blank group were not irradiated. After that, bone marrow stem cells 1x107 in 0.2ml normal saline were injected into mice by tail veins in test group, while 0.2ml normal saline (NS) was injected into mice by tail vein in control group. The peripheral blood was subjected to routine blood test on days 15, 25, 35 after transplantation, T lymphocytes (CD3~+, CD4~+, CD8~+) were determined by flow cytometry (FCM) on days15, 25, 35 after transplantation, and the concentration of mice peripheral blood immunoglobulins Ig M were measured by the ELISA method. All the mice were observed for survivals for 35 days. Re s ults The survival rates of mice 35 d after Hematopoietic stem cell transplantation were 80%, while 10% in control group. The leukocyte counts and Lymph% as well as CD3~+% in peripheral blood after transplantation increased as compared with those received NS (P <0.05) . However, CD4~+%, CD8~+% and serum immunoglobulin Ig M after transplantation decreased, which decreased remarkably on day 15 and begun to recover after that day. BMSC decreased Mono% and CD4~+/CD8~+in peripheral blood (P <0.05) .Conclus ion Bone marrow stem cell transplantation can repair the suppressed immunity of mice.
2017, 38(06): 15-18.
Abstract:
Objective To build animal model of hyperhomocysteinemia (HHcy) , and to investigate the expression of TRB3 in adipose tissue and explore the effect of PI3K/Akt signaling pathway by TRB3.Me thods Twenty mice were divided into the fasting control group (n = 10) and the fasting HHcy group (n = 10) . Normal food was fed in the fasting control group. Normal food and 1.5% (mass fraction) methionine were fed in the fasting HHcy group.After the mice in every group were fed for three months.Adipose tissue in two groups was The m RNA expressions of TRB3, Akt and glucose transporter 4 (GLUT4) were detected by reverse transcription PCR.The protein expression of TRB3, Akt, p-Akt (473) and GLUT4 were measured by Western blot. Re s ults The m RNA expression of TRB3 in the fast HHcy group was increasedas compared with the control group. The m RNA expression of Akt and GLUT4 was decreased, however the result had no significant difference between two groups.Compared with the control group, the protein expression of TRB3 was increased and the protein expression of p-Akt (473) was reduced in the fast HHcy group. Whereas the protein expression of Akt and GLUT4 had no significant diffenence between two groups. Conclus ion Homocysteine may incease the uptake and utilization of glucose in adipose tissue by inhibiting PI3K/Akt signaling pathway, which may be the results of inducing the expression of TRB3 and downregulating the protein expression of p-Akt (473) and GLUT4.
Objective To build animal model of hyperhomocysteinemia (HHcy) , and to investigate the expression of TRB3 in adipose tissue and explore the effect of PI3K/Akt signaling pathway by TRB3.Me thods Twenty mice were divided into the fasting control group (n = 10) and the fasting HHcy group (n = 10) . Normal food was fed in the fasting control group. Normal food and 1.5% (mass fraction) methionine were fed in the fasting HHcy group.After the mice in every group were fed for three months.Adipose tissue in two groups was The m RNA expressions of TRB3, Akt and glucose transporter 4 (GLUT4) were detected by reverse transcription PCR.The protein expression of TRB3, Akt, p-Akt (473) and GLUT4 were measured by Western blot. Re s ults The m RNA expression of TRB3 in the fast HHcy group was increasedas compared with the control group. The m RNA expression of Akt and GLUT4 was decreased, however the result had no significant difference between two groups.Compared with the control group, the protein expression of TRB3 was increased and the protein expression of p-Akt (473) was reduced in the fast HHcy group. Whereas the protein expression of Akt and GLUT4 had no significant diffenence between two groups. Conclus ion Homocysteine may incease the uptake and utilization of glucose in adipose tissue by inhibiting PI3K/Akt signaling pathway, which may be the results of inducing the expression of TRB3 and downregulating the protein expression of p-Akt (473) and GLUT4.
2017, 38(06): 19-24.
Abstract:
Objective To investigate a safe and effective clinical-grade cryoprotectant, which can be infused to patients. Me thods We collected 50 samples of cord blood using hydroxyethyl starch precipitation method to get cord blood nucleated cells. Then we added five kinds of different concentration cryoprectants including DMSO, human serum albumin and Plasmalyte A to cryopreserve nucleated cells in liquid nitrogen. The effects of these cryoprotectants on biological properties of cord blood nucleated cells were monitored and compared after the cells were refrigerated for six months. The detected biological properties included the number of total nucleated cells, CD34+ cells, colony forming units, recovery rate and trypan blue rejection rate. Cryoprotectants of different concentrations were injected into the mice of each group via tail vein, and the liver and renal toxicity were evaluated.Re s ults There was no difference between group A and group C which resulted in higher recovery rates of nucleated cells, CD34+ cells and colony forming units and trypan blue rejection rate than other three groups.The liver toxicity of group A were higher than other groups. Conclus ion The cryoprotectants with 7.5% DMSO +10% human serum albumin+ Plasmalyte A is safe and effective for cryopreserving the nucleated cells.
Objective To investigate a safe and effective clinical-grade cryoprotectant, which can be infused to patients. Me thods We collected 50 samples of cord blood using hydroxyethyl starch precipitation method to get cord blood nucleated cells. Then we added five kinds of different concentration cryoprectants including DMSO, human serum albumin and Plasmalyte A to cryopreserve nucleated cells in liquid nitrogen. The effects of these cryoprotectants on biological properties of cord blood nucleated cells were monitored and compared after the cells were refrigerated for six months. The detected biological properties included the number of total nucleated cells, CD34+ cells, colony forming units, recovery rate and trypan blue rejection rate. Cryoprotectants of different concentrations were injected into the mice of each group via tail vein, and the liver and renal toxicity were evaluated.Re s ults There was no difference between group A and group C which resulted in higher recovery rates of nucleated cells, CD34+ cells and colony forming units and trypan blue rejection rate than other three groups.The liver toxicity of group A were higher than other groups. Conclus ion The cryoprotectants with 7.5% DMSO +10% human serum albumin+ Plasmalyte A is safe and effective for cryopreserving the nucleated cells.
2017, 38(06): 25-27.
Abstract:
Objective To explore the basic situation of the quality of life of rural elderly people of different gender WA, provide the basis for improving the quality of life of ethnic minority elderly. Me thods SF-36 scale was used to measure the quality of life of 248 old people. The SPSS statistical software package was used for statistical description, t test and so on. Re s ults Comparison of life quality score between different gender: the total score, physiological score and psychological score, general health, mental health, physiological function, physiological function scores were different, all were higher in men than women (P<0.05) . Conclus ion The quality of life of rural elderly wa men have better quality of life than women.
Objective To explore the basic situation of the quality of life of rural elderly people of different gender WA, provide the basis for improving the quality of life of ethnic minority elderly. Me thods SF-36 scale was used to measure the quality of life of 248 old people. The SPSS statistical software package was used for statistical description, t test and so on. Re s ults Comparison of life quality score between different gender: the total score, physiological score and psychological score, general health, mental health, physiological function, physiological function scores were different, all were higher in men than women (P<0.05) . Conclus ion The quality of life of rural elderly wa men have better quality of life than women.
2017, 38(06): 28-31.
Abstract:
Objective To investigate the clinical significance of Tiam1 and Fascin protein expression in esophagus squamous carcinoma.Me thods Immunohistochemical SP method was used to detect Tiam1, Fascin protein in esophageal squamous carcinoma and normal esophageal tissue. Re s ults Tiam1, Fascin protein expression in esophageal squamous carcinoma tissues was significantly higher in the expression of normal esophageal tissue (P<0.05) , which was correlated with TNM stage, infiltration degree, lymphatic metastasis.Tiam1 protein expression was related to pathological grading of esophageal squamous carcinoma., and Fascin protein expression has no relation with the pathological grading of esophageal squamous carcinoma.There was close correlation between Tiam1 protein and Fascin protein (r = 0.302, P <0.01) . Conclus ions The higher expression of Tiam1 and Fascin in esophageal squamous carcinoma, are closely associated with invasion and metastasis. Tiam1 and Fascin may synergy effect in the process of esophageal squamous carcinoma. Tiam1, Fascin may become new targets in the therapy of esophagus squamous carcinoma.
Objective To investigate the clinical significance of Tiam1 and Fascin protein expression in esophagus squamous carcinoma.Me thods Immunohistochemical SP method was used to detect Tiam1, Fascin protein in esophageal squamous carcinoma and normal esophageal tissue. Re s ults Tiam1, Fascin protein expression in esophageal squamous carcinoma tissues was significantly higher in the expression of normal esophageal tissue (P<0.05) , which was correlated with TNM stage, infiltration degree, lymphatic metastasis.Tiam1 protein expression was related to pathological grading of esophageal squamous carcinoma., and Fascin protein expression has no relation with the pathological grading of esophageal squamous carcinoma.There was close correlation between Tiam1 protein and Fascin protein (r = 0.302, P <0.01) . Conclus ions The higher expression of Tiam1 and Fascin in esophageal squamous carcinoma, are closely associated with invasion and metastasis. Tiam1 and Fascin may synergy effect in the process of esophageal squamous carcinoma. Tiam1, Fascin may become new targets in the therapy of esophagus squamous carcinoma.
2017, 38(06): 32-35.
Abstract:
Objective To investigate the correlation between the age of onset and acute coronary syndrome (ACS) . Methods A total of 300 patients with ACS diagnosed in our hospital from June 2014 to June 2016 were selected in this study. Their age, history of smoking, history of obesity, history of hypertension and history of diabetes were recorded and analyzed statistically. Re s ults The results showed that the average age of onset of ACS was higher than that of other ACS patients without exposure to smoking, obesity, hypertension, diabetes and other risk factors, the differences was statically significant (P<0.05) . The mean age at onset of ACS, when exposed to two or more risk factors, was earlier than that of exposure to one or no risk factors, the differences was statically significant (P <0.05) .Conclus ions Smoking, obesity, diabetes and hypertension are risk factors of ACS, and the onset age of patients with ACS may be moved up significantly. Combined with the more factors above, the patients with ACS are more likely to advance the age of onset. Early control of smoking, obesity, diabetes, high blood pressure, is particularly important to the prevention of ACS.
Objective To investigate the correlation between the age of onset and acute coronary syndrome (ACS) . Methods A total of 300 patients with ACS diagnosed in our hospital from June 2014 to June 2016 were selected in this study. Their age, history of smoking, history of obesity, history of hypertension and history of diabetes were recorded and analyzed statistically. Re s ults The results showed that the average age of onset of ACS was higher than that of other ACS patients without exposure to smoking, obesity, hypertension, diabetes and other risk factors, the differences was statically significant (P<0.05) . The mean age at onset of ACS, when exposed to two or more risk factors, was earlier than that of exposure to one or no risk factors, the differences was statically significant (P <0.05) .Conclus ions Smoking, obesity, diabetes and hypertension are risk factors of ACS, and the onset age of patients with ACS may be moved up significantly. Combined with the more factors above, the patients with ACS are more likely to advance the age of onset. Early control of smoking, obesity, diabetes, high blood pressure, is particularly important to the prevention of ACS.
2017, 38(06): 36-39.
Abstract:
目的 探讨不同类型的非生物型人工肝技术对肝衰竭合并肝肾综合征的临床疗效.方法 选取2014年1月至2015年12月在昆明市第三人民医院住院的肝功能衰竭合并肝肾综合征患者60例作为研究对象, 共行人工肝治疗317人次.入院后根据治疗方案的不同分为观察组和对照组, 每组30例, 观察组给予血浆置换联合血液透析滤过 (plasma exchange+hemo-diafiltration, PE+HDF) 治疗, 对照组给予血浆置换 (plasma exchange, PE) 治疗.观察2组治疗后肝肾功能、凝血功能及电解质的变化及近期疗效.结果 (1) 治疗后, 观察组血清总胆红素、尿素氮、肌酐、钾等指标水平明显低于对照组, 差异有统计学意义 (P<0.05) ;观察组凝血酶原活动度指标明显上升, 且高于对照组, 差异有统计学意义 (P<0.05) ; (2) 在近期疗效方面, 观察组达到78.95%, 高于对照组 (50%) , 差异有统计学意义 (P<0.05) .结论 相对血浆置换来说, 血浆置换联合血液透析治疗肝衰竭合并肝肾综合征方面更具优势, 能够有效改善肝肾功能及维持电解质平衡, 近期治疗有效率更高.
2017, 38(06): 40-43.
Abstract:
Objective To investigate the incidence of nosocomial infection in ICU, mortality rate of infected patients, the risk-factors and strategies of nosocomial infection. Me thods We retrospectively analyzed all ICU patients' medical records from August 2015 to August 2016, and the incidence of nosocomial infection. All patients were retrospectively analyzed, the infected patients as the observation group, the same period without infection as a control group. We compared the gender, age, APACHEII score, admission ICU time, plasma albumin levels, whether surgery, whether there were disturbance of consciousness, whether invasive operation, whether the use of antibiotics, whether combined with other underlying diseases and other risk factors between the two groups of patients. We also compared the differences in mortality between the two groups of patients. Re s ults The incidence of nosocomial infection was 29.41%. There were significant differences in APACHEII score, ICU time, disturbance of consciousness, underlying disease, age and invasive operation (P <0.05) . There was no statistically significant difference in sex, plasma albumin level, operation and prophylactic use of antibiotics significance. The mortality rate (40%) was significantly higher in hospital patients than in the control group (16.7%) (P<0.05) . Conclusion Severity of illness, ICU stay, unconsciousness, underlying disease, age, invasive procedures have a significant impact on the incidence of nosocomial infections, gender, serum albumin level, surgery, prophylactic use of antibiotics have no effect on the incidence of nosocomial infections.
Objective To investigate the incidence of nosocomial infection in ICU, mortality rate of infected patients, the risk-factors and strategies of nosocomial infection. Me thods We retrospectively analyzed all ICU patients' medical records from August 2015 to August 2016, and the incidence of nosocomial infection. All patients were retrospectively analyzed, the infected patients as the observation group, the same period without infection as a control group. We compared the gender, age, APACHEII score, admission ICU time, plasma albumin levels, whether surgery, whether there were disturbance of consciousness, whether invasive operation, whether the use of antibiotics, whether combined with other underlying diseases and other risk factors between the two groups of patients. We also compared the differences in mortality between the two groups of patients. Re s ults The incidence of nosocomial infection was 29.41%. There were significant differences in APACHEII score, ICU time, disturbance of consciousness, underlying disease, age and invasive operation (P <0.05) . There was no statistically significant difference in sex, plasma albumin level, operation and prophylactic use of antibiotics significance. The mortality rate (40%) was significantly higher in hospital patients than in the control group (16.7%) (P<0.05) . Conclusion Severity of illness, ICU stay, unconsciousness, underlying disease, age, invasive procedures have a significant impact on the incidence of nosocomial infections, gender, serum albumin level, surgery, prophylactic use of antibiotics have no effect on the incidence of nosocomial infections.
2017, 38(06): 44-47.
Abstract:
Objective To explore the clinical efficacy of Ilizarov technique in treatment of Tibial infected bone defect, and analyze the best indications. Me thods We retrospectively analysed the data of 15 cases of tibial infected bone defect, from January 2013 to January 2017. The length of bone defect ranged from 4-7cm, with an average of 5.6cm. All patients were treated by debridement, Ilizarov external fixation, immediately osteotomy, bone transport. Then we recorded the postoperative healing rate, complications and function, and evaluated by Paley criteria. Re s ults Their follow-up periods ranged from 11 to 42 months (mean, 28.4 months) . Postoperative X-ray radiography examination was taken Regularly to evaluate bone healing process, healing time 5 ~ 13 months, the average 8.6 months. There was no recurrence of tibia infection, skin dragging pain was found in 7 cases, malposition in 2 cases. The Limb length recovered well, and adjacent joints, function was good. According to Paley criteria for management of infectious tibial defect, 7 cases were rated as excellent and 6 as good, the excellent rates was 86.7%. Conclus ion For treatment of tibial large infected bone defect associated with soft tissue defects, application of Ilizarov Technique can be effective.
Objective To explore the clinical efficacy of Ilizarov technique in treatment of Tibial infected bone defect, and analyze the best indications. Me thods We retrospectively analysed the data of 15 cases of tibial infected bone defect, from January 2013 to January 2017. The length of bone defect ranged from 4-7cm, with an average of 5.6cm. All patients were treated by debridement, Ilizarov external fixation, immediately osteotomy, bone transport. Then we recorded the postoperative healing rate, complications and function, and evaluated by Paley criteria. Re s ults Their follow-up periods ranged from 11 to 42 months (mean, 28.4 months) . Postoperative X-ray radiography examination was taken Regularly to evaluate bone healing process, healing time 5 ~ 13 months, the average 8.6 months. There was no recurrence of tibia infection, skin dragging pain was found in 7 cases, malposition in 2 cases. The Limb length recovered well, and adjacent joints, function was good. According to Paley criteria for management of infectious tibial defect, 7 cases were rated as excellent and 6 as good, the excellent rates was 86.7%. Conclus ion For treatment of tibial large infected bone defect associated with soft tissue defects, application of Ilizarov Technique can be effective.
2017, 38(06): 48-51.
Abstract:
Objective To evaluate diagnostic significance of single high frequency ultrasound and mammography and combined therapy of both on breast cancer. Me thods 355 cases of female breast cancer were selected from The First Hospital of Kunming from December 2015 to December 2016. Among these 355 patients, 125 cases had only performed high frequency ultrasound, 103 cases had only performed breast X-ray radiography, 127 cases of conducted the therapy of both. Re s ults The coincidence rate of singlebreast X-ray was 79.4%, the misdiagnosis rate was 10.7%, the missed diagnosis rate was 9.8%. The coincidence rate of single high-frequency ultrasonic testis80.0%, the misdiagnosis rate was 11.2%, the missed diagnosis rate was 4.6%; the coincidence rate of combination of two test was 89.7%, the misdiagnosis rate was 6.3%, the missed diagnosis rate was 4.0%. The detection rate and the missed diagnosis of joint diagnosis had statistical difference with single high frequency ultrasound and single mammography.There was no statistical difference on misdiagnosis rate. Conclus ions Both high frequency ultrasound and mammography has their own advantages respectively. The joint application of both has better diagnosis complementary, and can significantly improve the detection rate and the accuracy rate of breast cancer, decrease the rate of misdiagnosis and missed diagnosis rate.
Objective To evaluate diagnostic significance of single high frequency ultrasound and mammography and combined therapy of both on breast cancer. Me thods 355 cases of female breast cancer were selected from The First Hospital of Kunming from December 2015 to December 2016. Among these 355 patients, 125 cases had only performed high frequency ultrasound, 103 cases had only performed breast X-ray radiography, 127 cases of conducted the therapy of both. Re s ults The coincidence rate of singlebreast X-ray was 79.4%, the misdiagnosis rate was 10.7%, the missed diagnosis rate was 9.8%. The coincidence rate of single high-frequency ultrasonic testis80.0%, the misdiagnosis rate was 11.2%, the missed diagnosis rate was 4.6%; the coincidence rate of combination of two test was 89.7%, the misdiagnosis rate was 6.3%, the missed diagnosis rate was 4.0%. The detection rate and the missed diagnosis of joint diagnosis had statistical difference with single high frequency ultrasound and single mammography.There was no statistical difference on misdiagnosis rate. Conclus ions Both high frequency ultrasound and mammography has their own advantages respectively. The joint application of both has better diagnosis complementary, and can significantly improve the detection rate and the accuracy rate of breast cancer, decrease the rate of misdiagnosis and missed diagnosis rate.
2017, 38(06): 52-55.
Abstract:
Objective To establish an automatic typing method for MAOA μ-VNTR polymorphism test using capillary electrophoresis and fluorescence labeling techniques. Me thods DNAwas extracted by Chelex-100 method. Blood samples from 720 unrelated Chinese Han individuals in Yunnan were genotyped by capillary electrophoresis and fluorescence labeled primer. Re s ults MAOA μ-VNTR polymorphism fluorescent labeling automatic typing method had high efficiency. And the results from this method were accurate and reliable.Conclus ions The operation of MAOA μ-VNTR polymorphism fluorescent labeling automatic typing method is simple. It has good prospects in the research for the MAOA μ-VNTR polymorphism test.
Objective To establish an automatic typing method for MAOA μ-VNTR polymorphism test using capillary electrophoresis and fluorescence labeling techniques. Me thods DNAwas extracted by Chelex-100 method. Blood samples from 720 unrelated Chinese Han individuals in Yunnan were genotyped by capillary electrophoresis and fluorescence labeled primer. Re s ults MAOA μ-VNTR polymorphism fluorescent labeling automatic typing method had high efficiency. And the results from this method were accurate and reliable.Conclus ions The operation of MAOA μ-VNTR polymorphism fluorescent labeling automatic typing method is simple. It has good prospects in the research for the MAOA μ-VNTR polymorphism test.
2017, 38(06): 56-59.
Abstract:
Objective To study the dust mites species and predominant species in Kunming, to provide evidence for the prevention and cure of dust mite allergy. Me thods From August 2010 to August 2011, mite-allergic patients, including allergic rhinitis and asthma of the patients who visited the The 1st Affiliated Hospital of Kunming Medical University. Dust samples from the patients' homes were collected by vacuuming, and mites in the samples were isolated with the flotation method. Permanent slides were prepared for morphologically based species determination. Re s ults In total 4275 mite specimens of morphologically identifiable species were found in 287 dust samples taken from 35 homes. 71.1% samples were infested with mites. The result showed that the mite family of Pyroglyphidae occupied the highest percentage (97.2%) of the total amount of mites collected. The most common adult Pyroglyphidae mites were Dermatophagoides (D.) farinae (74.9%) , D. pteronyssinus (23.6%) , and D. Blomiatropicalis (1.9%) . The mite density was 121.4mites/g in wet season and 77.3mites/g in dry season (P<0.05) and the highest in August through September, the lowest from January to march. Conclusion The predominant species of mite in Kunming region is dust mites, followed by house dust mites and the blomiatropicalis. The mean density of dust mites is the highest in August to September.
Objective To study the dust mites species and predominant species in Kunming, to provide evidence for the prevention and cure of dust mite allergy. Me thods From August 2010 to August 2011, mite-allergic patients, including allergic rhinitis and asthma of the patients who visited the The 1st Affiliated Hospital of Kunming Medical University. Dust samples from the patients' homes were collected by vacuuming, and mites in the samples were isolated with the flotation method. Permanent slides were prepared for morphologically based species determination. Re s ults In total 4275 mite specimens of morphologically identifiable species were found in 287 dust samples taken from 35 homes. 71.1% samples were infested with mites. The result showed that the mite family of Pyroglyphidae occupied the highest percentage (97.2%) of the total amount of mites collected. The most common adult Pyroglyphidae mites were Dermatophagoides (D.) farinae (74.9%) , D. pteronyssinus (23.6%) , and D. Blomiatropicalis (1.9%) . The mite density was 121.4mites/g in wet season and 77.3mites/g in dry season (P<0.05) and the highest in August through September, the lowest from January to march. Conclusion The predominant species of mite in Kunming region is dust mites, followed by house dust mites and the blomiatropicalis. The mean density of dust mites is the highest in August to September.
2017, 38(06): 60-63.
Abstract:
目的 探讨武汉地区冠心病患者药物代谢酶CYP2C19基因多态性的分布.方法 采用基因芯片法对123例服用氯吡格雷的无血缘关系的冠心病患者进行CYP2C19基因分型, 分析其基因型及代谢表型的分布, 并与文献报道中中国汉族人群进行比较.结果 共统计发现5种基因型, 3种代谢表型;检测出快代谢型 (EM) 患者38例, 所占比例为30.9%, 检测出中间代谢型 (IM) 患者69例, 所占比例为56.1%, 检测出慢代谢型 (PM) 患者16例, 所占比例为13.0%.武汉地区冠心病患者中, CYP2C19基因代谢表型分布与文献报道无统计学意义 (P>0.05) .结论 武汉地区冠心病患者中, CYP2C19基因存在多态性, 基因频率及代谢型分布频率与国内其他地区相一致.
2017, 38(06): 64-67.
Abstract:
Objective To study the changes in the composition of main lymphocytes in peripheral blood of patients with AECOPD (acute exacerbation of chronic obstructive pulmonary disease) . Me thods From January1 th, 2014 to 31 th December, total 789 admitted patients with AECOPD received immunologic function test. Their age ranged from 45 to 96 years old (76.33 ±9.56) . Mean ±SD was used in statistics and groups difference was analyzed by one way analysis of variance. Re s ults The data of 789 samples showed that CD3+ T lymphocyte subpopulation mean value was 60.07 ±13.11, which was lower than normal parameter. There was significant differences among different age groups (F3.410, P0.009) .According to statistics of different age groups, mean value of CD3+ T lymphocyte subpopulation was lower than normal parameter in patients with age ≥60y. The mean value changes of other lymphocyte subpopulation were in normal range. Conclus ions There is lower immunology function in patients with senile AECOPD, and the value of CD3+ T lymphocyte is significant difference among different age groups.
Objective To study the changes in the composition of main lymphocytes in peripheral blood of patients with AECOPD (acute exacerbation of chronic obstructive pulmonary disease) . Me thods From January1 th, 2014 to 31 th December, total 789 admitted patients with AECOPD received immunologic function test. Their age ranged from 45 to 96 years old (76.33 ±9.56) . Mean ±SD was used in statistics and groups difference was analyzed by one way analysis of variance. Re s ults The data of 789 samples showed that CD3+ T lymphocyte subpopulation mean value was 60.07 ±13.11, which was lower than normal parameter. There was significant differences among different age groups (F3.410, P0.009) .According to statistics of different age groups, mean value of CD3+ T lymphocyte subpopulation was lower than normal parameter in patients with age ≥60y. The mean value changes of other lymphocyte subpopulation were in normal range. Conclus ions There is lower immunology function in patients with senile AECOPD, and the value of CD3+ T lymphocyte is significant difference among different age groups.
2017, 38(06): 68-72.
Abstract:
Objective To investigate the risk factors of myocardial infarction in Han population from Yunnan province. Me thods 500 myocardial infarction patients and 350 non CHD patients were selected in the affiliated Yanan Hospital of Kunming Medical University from October 2013 to December 2015.These patients were divided into young female group, aged female group, male group and aged male group by age and gende.CAD/MI possible risk factors were collected. Re s ults Diabetes and family history of CAD were risk factors in young women MI patients. Hypertension and diabetes were risk factors in elderly women MI patients. Family history of CAD, obesity and smoking were risk factors in young male MI patients. Obesity, diabetes and LDL were risk factors in elderly men MI patients. Conclus ion The risk factors of CAD/MI are closely related to the age and gender of the patients, and the preventive measures should be taken according to the age and gender to reduce the incidence of CAD/MI.
Objective To investigate the risk factors of myocardial infarction in Han population from Yunnan province. Me thods 500 myocardial infarction patients and 350 non CHD patients were selected in the affiliated Yanan Hospital of Kunming Medical University from October 2013 to December 2015.These patients were divided into young female group, aged female group, male group and aged male group by age and gende.CAD/MI possible risk factors were collected. Re s ults Diabetes and family history of CAD were risk factors in young women MI patients. Hypertension and diabetes were risk factors in elderly women MI patients. Family history of CAD, obesity and smoking were risk factors in young male MI patients. Obesity, diabetes and LDL were risk factors in elderly men MI patients. Conclus ion The risk factors of CAD/MI are closely related to the age and gender of the patients, and the preventive measures should be taken according to the age and gender to reduce the incidence of CAD/MI.
2017, 38(06): 73-76.
Abstract:
Objective To investigate the effect of pulmonary dead-space fraction on the prognosis of acute respiratory distress syndrome (ARDS) . Me thods 32 patients meeting ARDS Berlin definition, who were admitted to the EICU from January 2015 to August 2016, were analyzed with a prospective method. The VD/VT was measured by the single breath test of CO2. (day6 from day1) . Patients' general condition, vital signs, APACHEⅡ, LIPS, Pa O2/Fi O2, 28-day mortality were recorded.The patients were divided into a survival group and a death group according to their survival situation in 28 days. Re s ults Comparison of VD/VT between the death group and the survival group of ARDS patients at different time point: VD/VT was elevated in the two groups.VD/VT showed no statistical difference from the 1st day to the 3th day after admission, but higher in the death group from the 4th day to the 6th day after admission (P<0.01) .VD/VT was significantly higher in non survivors than in survivors from the 4th day of mechanical ventilation. ROC curve and 28-day survival curve showed that the area under ROC curve of VD/VT predicting prognosis of patients with ARDS was 0.910, higher than APACHEⅡand LIPS. When the optimal cut off value was 0.62, the sensitivity was 0.916, and specificity was 0.955. Conclus ion VD/VT is a risk factor of patients 28 day prognosis, VD/VT of the 4th day of ARDS is associated with greater risk of death.
Objective To investigate the effect of pulmonary dead-space fraction on the prognosis of acute respiratory distress syndrome (ARDS) . Me thods 32 patients meeting ARDS Berlin definition, who were admitted to the EICU from January 2015 to August 2016, were analyzed with a prospective method. The VD/VT was measured by the single breath test of CO2. (day6 from day1) . Patients' general condition, vital signs, APACHEⅡ, LIPS, Pa O2/Fi O2, 28-day mortality were recorded.The patients were divided into a survival group and a death group according to their survival situation in 28 days. Re s ults Comparison of VD/VT between the death group and the survival group of ARDS patients at different time point: VD/VT was elevated in the two groups.VD/VT showed no statistical difference from the 1st day to the 3th day after admission, but higher in the death group from the 4th day to the 6th day after admission (P<0.01) .VD/VT was significantly higher in non survivors than in survivors from the 4th day of mechanical ventilation. ROC curve and 28-day survival curve showed that the area under ROC curve of VD/VT predicting prognosis of patients with ARDS was 0.910, higher than APACHEⅡand LIPS. When the optimal cut off value was 0.62, the sensitivity was 0.916, and specificity was 0.955. Conclus ion VD/VT is a risk factor of patients 28 day prognosis, VD/VT of the 4th day of ARDS is associated with greater risk of death.
2017, 38(06): 77-81.
Abstract:
Objective To evaluate the short-term curative effect of PKRP and Tm LRP for benign prostatic hyperplasia (BPH) . Me thods We selected 100 subjects who would like to accept surgical treatment, and then randomly divided them into two groups (PKRP and Tm LRP) , 50 in each group. Then we compared the recent curative effect between two different surgical methods. Re s ults The mean catheterization, irrigation and hospital stay time in the Tm LRP were shorter than those in the PKRP group respectively (P < 0.05) . The general results were satisfactory. The IPSS, QOLS, RUV and Qmax were impoved significantly (P < 0.01) , and the differences between groups had no statistical significance (P > 0.05) . Statistically significant difference was found in the operative time and postoperative complications between Tm LRP and PKRP with operative time 61.2 ±24.2 minutes and 30.1±15.9 minutes respectively (P < 0.05) whereas postoperative complications using Tm LRP treatment were less than PKRP (P < 0.05) . Conclus ion Despite longer operative time, Tm LRP treatment shows multiple advantages, such as more precise prostate intraoperative anatomic resection, less bleeding, shorter recovery period, lesser pain, shorter hospitalization, lower short-term complication rates, higher security, etc, and has similar short-term curative effect to PKRP treatment.
Objective To evaluate the short-term curative effect of PKRP and Tm LRP for benign prostatic hyperplasia (BPH) . Me thods We selected 100 subjects who would like to accept surgical treatment, and then randomly divided them into two groups (PKRP and Tm LRP) , 50 in each group. Then we compared the recent curative effect between two different surgical methods. Re s ults The mean catheterization, irrigation and hospital stay time in the Tm LRP were shorter than those in the PKRP group respectively (P < 0.05) . The general results were satisfactory. The IPSS, QOLS, RUV and Qmax were impoved significantly (P < 0.01) , and the differences between groups had no statistical significance (P > 0.05) . Statistically significant difference was found in the operative time and postoperative complications between Tm LRP and PKRP with operative time 61.2 ±24.2 minutes and 30.1±15.9 minutes respectively (P < 0.05) whereas postoperative complications using Tm LRP treatment were less than PKRP (P < 0.05) . Conclus ion Despite longer operative time, Tm LRP treatment shows multiple advantages, such as more precise prostate intraoperative anatomic resection, less bleeding, shorter recovery period, lesser pain, shorter hospitalization, lower short-term complication rates, higher security, etc, and has similar short-term curative effect to PKRP treatment.
2017, 38(06): 82-86.
Abstract:
Objective To investigate the diagnostic value and correlation of corresponding laboratory indexes in rheumatoid arthritis (RA) complicated with coronary heart disease (CHD) . Me thods According to gender and age, patients were divided into RA concurrent CHD group, simple RA group, simple CHD group and healthy control group, with 102 cases in each group. The Level change of RA specificity index (ESR, RF, ACCP, IL-6) and CHD specificity index (CK, CK-MB, α-HBDH, hs-Tn T, Mb) in the four groups were analyzed retrospectively, and we statistically analyzed the correlation between the two groups of specific indicators in RA complicated with CHD group. Re s ults The rate of joint deformity in RA sick group (including simple and concurrent) was significantly higher than that in non-RA group (P =0.000) , the hypertension prevalence and smoking rate in simple CHD group were significantly higher than the other three groups (P=0.016, 0.021) , there was no significant difference in BMI and TC between the four groups (P>0.05) In patients with RA complicated with CHD, the RA specificity index of ESR, RF, ACCP and IL-6, and CKD specificity index of CK, CK-MB, hs-Tn T and Mb were significantly increased (P <0.05) . ACCP was positively correlated with CK and CK-MB (P <0.05) , IL-6 was positively correlated with CK, CK-MB and Mb (P <0.05) . Conclus ion The detection of ACCP or IL-6 in RA patients can predict the incidence of RA complicated with CHD.
Objective To investigate the diagnostic value and correlation of corresponding laboratory indexes in rheumatoid arthritis (RA) complicated with coronary heart disease (CHD) . Me thods According to gender and age, patients were divided into RA concurrent CHD group, simple RA group, simple CHD group and healthy control group, with 102 cases in each group. The Level change of RA specificity index (ESR, RF, ACCP, IL-6) and CHD specificity index (CK, CK-MB, α-HBDH, hs-Tn T, Mb) in the four groups were analyzed retrospectively, and we statistically analyzed the correlation between the two groups of specific indicators in RA complicated with CHD group. Re s ults The rate of joint deformity in RA sick group (including simple and concurrent) was significantly higher than that in non-RA group (P =0.000) , the hypertension prevalence and smoking rate in simple CHD group were significantly higher than the other three groups (P=0.016, 0.021) , there was no significant difference in BMI and TC between the four groups (P>0.05) In patients with RA complicated with CHD, the RA specificity index of ESR, RF, ACCP and IL-6, and CKD specificity index of CK, CK-MB, hs-Tn T and Mb were significantly increased (P <0.05) . ACCP was positively correlated with CK and CK-MB (P <0.05) , IL-6 was positively correlated with CK, CK-MB and Mb (P <0.05) . Conclus ion The detection of ACCP or IL-6 in RA patients can predict the incidence of RA complicated with CHD.
2017, 38(06): 87-91.
Abstract:
Objective To observe the analgesic effect of ultrasound-guided paravertebral nerve block on analgesia after thoracotomy. Me thods 70 patients who were in ASA Ⅰ ~ Ⅱ and planned to undergo elective thoracotomy surgery with tracheal intubation anesthesia were randomly divided into two groups (Group T and Group G) of 35 cases each. Patients in group T received paravertebral injection of 0.5% of pivocaine under ultrasound guide before induction. Postoperative intravenous self-control analgesia pump was used in all patients. The patients' heart rate (HR) and mean arterial pressure (MAP) and Oxygen saturation air condition (Sp O2) were recorded in different moments. The visual analog scale (VAS) scores were recorded at 6, 12, 24 and 48 h after operation.Frequency for compress PCA, related complications, the first eating time and the leaving bed time after operation were recorded as well. Re s ults At 6, 12 and 24 h after operation, the change in HR, MAP and VAS scale of Group T varied within a smaller range than Group G (P<0.05) . The PCA numbers, postoperative nausea and vomiting were significantly less in group P than those in group G (P<0.05) .The time of the first eating and the leaving bed after operation was shorten in Group T than in Group G (P <0.05) . Conclus ion Thoracic paravertebral block by ultrasound guide is accurate and effective for elective thoracotomy patient, can effectively reduce postoperative pain, less adverse reaction, is beneficial to patients with early rehabilitation, is the ideal for postoperative analgesia after thoracotomy.
Objective To observe the analgesic effect of ultrasound-guided paravertebral nerve block on analgesia after thoracotomy. Me thods 70 patients who were in ASA Ⅰ ~ Ⅱ and planned to undergo elective thoracotomy surgery with tracheal intubation anesthesia were randomly divided into two groups (Group T and Group G) of 35 cases each. Patients in group T received paravertebral injection of 0.5% of pivocaine under ultrasound guide before induction. Postoperative intravenous self-control analgesia pump was used in all patients. The patients' heart rate (HR) and mean arterial pressure (MAP) and Oxygen saturation air condition (Sp O2) were recorded in different moments. The visual analog scale (VAS) scores were recorded at 6, 12, 24 and 48 h after operation.Frequency for compress PCA, related complications, the first eating time and the leaving bed time after operation were recorded as well. Re s ults At 6, 12 and 24 h after operation, the change in HR, MAP and VAS scale of Group T varied within a smaller range than Group G (P<0.05) . The PCA numbers, postoperative nausea and vomiting were significantly less in group P than those in group G (P<0.05) .The time of the first eating and the leaving bed after operation was shorten in Group T than in Group G (P <0.05) . Conclus ion Thoracic paravertebral block by ultrasound guide is accurate and effective for elective thoracotomy patient, can effectively reduce postoperative pain, less adverse reaction, is beneficial to patients with early rehabilitation, is the ideal for postoperative analgesia after thoracotomy.
2017, 38(06): 92-96.
Abstract:
Objective To explore the relationship between acute exacerbation of chronic obstructive pulmonary disease (AECOPD) and pulmonary hypertension (PH) in patients with chronic obstructive pulmonary disease (COPD) . Me thods Using retrospective analysis, we collected from March 2015 to March 2016 in the department of respiratory internal medicine (ward two) in our hospital, 38 cases of AECOPD combined with pulmonary hypertension patients as a experimental group, 46 cases of simple AECOPD patients in the same period as the controlgroup. And we collected basic data. The serum of Interleukin-6 (IL-6) , Procalcitonin (PCT) , High sensitive C reactive protein (hs-CRP) , N-Terminal pro-Brain natriuretic peptide (NT-proBNP) were collected after admission within 24 hours, the results of Chest CT and Ultrasonic echocardiography. Compared with the control group and experimental group of two groups in the above indicators. Re s ults AECOPD combined with PH group of the smoking index, hs-CRP, PCT, NT-proBNP were higher than the simple AECOPD group (P <0.05) , and had positive correlation with pulmonary artery systolic pressure (P<0.05) . Logistic regression analysis showed that NT-proBNP was an independent risk factor for pulmonary arterial hypertension (OR=1.89, P<0.01) .Clolus ions Smoking and serum hs-CRP, PCT, NT-proB NP are positively correlated with elevated pulmonary artery pressure, NT-proB NP is an independent risk factor for pulmonary arterial hypertension. Smoking and systemic inflammation are involved in the formation of pulmonary hypertension in COPD.
Objective To explore the relationship between acute exacerbation of chronic obstructive pulmonary disease (AECOPD) and pulmonary hypertension (PH) in patients with chronic obstructive pulmonary disease (COPD) . Me thods Using retrospective analysis, we collected from March 2015 to March 2016 in the department of respiratory internal medicine (ward two) in our hospital, 38 cases of AECOPD combined with pulmonary hypertension patients as a experimental group, 46 cases of simple AECOPD patients in the same period as the controlgroup. And we collected basic data. The serum of Interleukin-6 (IL-6) , Procalcitonin (PCT) , High sensitive C reactive protein (hs-CRP) , N-Terminal pro-Brain natriuretic peptide (NT-proBNP) were collected after admission within 24 hours, the results of Chest CT and Ultrasonic echocardiography. Compared with the control group and experimental group of two groups in the above indicators. Re s ults AECOPD combined with PH group of the smoking index, hs-CRP, PCT, NT-proBNP were higher than the simple AECOPD group (P <0.05) , and had positive correlation with pulmonary artery systolic pressure (P<0.05) . Logistic regression analysis showed that NT-proBNP was an independent risk factor for pulmonary arterial hypertension (OR=1.89, P<0.01) .Clolus ions Smoking and serum hs-CRP, PCT, NT-proB NP are positively correlated with elevated pulmonary artery pressure, NT-proB NP is an independent risk factor for pulmonary arterial hypertension. Smoking and systemic inflammation are involved in the formation of pulmonary hypertension in COPD.
2017, 38(06): 97-101.
Abstract:
Objective To analyze the differentially expressed genes and signaling pathways in cisplatin resistant ovarian cancer. Me thods Cisplatin resistant ovarian cancer gene set GSE15372 was obtained from the GEO database of the national information center of the United States. The Differential expression gene analysis was performed by R and Bioconductor software, the GO and KEGG pathway of the differentially expressed genes were analyzed by DAVID online tool. Results 211 differentially expressed genes were obtained, including 120 up-and91 down-regulated genes as the standa rd was set that fold change was more than 2 times and FDR was less than0.05. Gene enrichment analysis of differentially expressed genes found in focal adhesion, TGF-beta signaling pathways and other biological processes (P<0.05) . Conclus ion There are some specific differentially expressed genes in the cisplatin resistant ovarian cancer, partial signal pathways were obvious enrichment in the cisplatin resistant ovarian cancer.
Objective To analyze the differentially expressed genes and signaling pathways in cisplatin resistant ovarian cancer. Me thods Cisplatin resistant ovarian cancer gene set GSE15372 was obtained from the GEO database of the national information center of the United States. The Differential expression gene analysis was performed by R and Bioconductor software, the GO and KEGG pathway of the differentially expressed genes were analyzed by DAVID online tool. Results 211 differentially expressed genes were obtained, including 120 up-and91 down-regulated genes as the standa rd was set that fold change was more than 2 times and FDR was less than0.05. Gene enrichment analysis of differentially expressed genes found in focal adhesion, TGF-beta signaling pathways and other biological processes (P<0.05) . Conclus ion There are some specific differentially expressed genes in the cisplatin resistant ovarian cancer, partial signal pathways were obvious enrichment in the cisplatin resistant ovarian cancer.
2017, 38(06): 102-107.
Abstract:
Objective To monitor placental function by using the ultrasonic blood flow index and serumβ-HCG.Me thods From May 2012 to December 2015 in our hospital, 1053 cases of prenatal puerperas were given placenta vascularization index (VI) , blood flow index (FI) and vascularization-blood flow index (VFI) measurement by GE E8 three-dimensional ultrasound at the same time, received β-HCG levels test by using the method of chemiluminescence. Postpartum neonatal body length, body weight and vital signs comprehensive score (Apgar score) were also recorded.Re s ults There were 131 cases with serum freeβ-HCGMo M≥2.5, 123 cases with neonatal weight < 2 500 g, 8 cases with normal weight; 542 cases with 2.0 <β-HCGMo M < 2.5, 4 cases with neonatal weight < 2500 g, 438 cases with normal weight; 425 cases with 1.5 <β-HCG Mo M<2.0, normal weight newborn; 59 cases with β-HCG Mo Mvalues <1.5, normal birth weight. The incidence of intrauterine retardation was 93.8% in puerperas with β-HCGMo M≥2.5, 0.7% in puerperas with 2.0<β-HCGMo M < 2.0, and 0% in puerperas with 1.5<β-HCGMo M < 2.0 and β-HCGMo M < 1.5. The incidence of intrauterine retardation in the group of pregnant women with serum free β-HCGMo M≥2.5 was significantly greater than the control group (P < 0.05) . The placental villi vascular tree of 1053 cases was reconstructed by 3 d power doppler technology, and the primary, secondary and tertiary villi rate of artery in 125 cases in the study group was100%, 73%, 38%, in 6 cases in the study group was 100%, 86% and 73% respectively, and in the control group, the primary, secondary and tertiary villi rate of artery was 100%, 100%, 85%, which were significantly higher than study group (P < 0.05) . Conclus ions When 2.5 > serum free β-HCGMo M of pregnant women > 2.0, the newborn may be associated with intrauterine retardation. Pregnant women serum freeβ-HCGMo M≥2.5, there is obviously increase in the incidence of neonatal intrauterine retardation. For those withintrauterine retardation, the placenta secondary and tertiary villi artery diameter is small, the number of artery decreases significantly. Three-dimensional power doppler blood flow index of VI, FI and VFI are significantly lower than normal. Serum free β-HCGMoM of pregnant women can predict the placenta function, villi vascular artery hierarchical display rate and the three-dimensional power doppler blood flow index can qualitatively and quantitatively determine the placental function. Placenta in mid gestation will be injured because of ischemia and anoxia, and the placental function can be recovered after placental vascular remodeling.
Objective To monitor placental function by using the ultrasonic blood flow index and serumβ-HCG.Me thods From May 2012 to December 2015 in our hospital, 1053 cases of prenatal puerperas were given placenta vascularization index (VI) , blood flow index (FI) and vascularization-blood flow index (VFI) measurement by GE E8 three-dimensional ultrasound at the same time, received β-HCG levels test by using the method of chemiluminescence. Postpartum neonatal body length, body weight and vital signs comprehensive score (Apgar score) were also recorded.Re s ults There were 131 cases with serum freeβ-HCGMo M≥2.5, 123 cases with neonatal weight < 2 500 g, 8 cases with normal weight; 542 cases with 2.0 <β-HCGMo M < 2.5, 4 cases with neonatal weight < 2500 g, 438 cases with normal weight; 425 cases with 1.5 <β-HCG Mo M<2.0, normal weight newborn; 59 cases with β-HCG Mo Mvalues <1.5, normal birth weight. The incidence of intrauterine retardation was 93.8% in puerperas with β-HCGMo M≥2.5, 0.7% in puerperas with 2.0<β-HCGMo M < 2.0, and 0% in puerperas with 1.5<β-HCGMo M < 2.0 and β-HCGMo M < 1.5. The incidence of intrauterine retardation in the group of pregnant women with serum free β-HCGMo M≥2.5 was significantly greater than the control group (P < 0.05) . The placental villi vascular tree of 1053 cases was reconstructed by 3 d power doppler technology, and the primary, secondary and tertiary villi rate of artery in 125 cases in the study group was100%, 73%, 38%, in 6 cases in the study group was 100%, 86% and 73% respectively, and in the control group, the primary, secondary and tertiary villi rate of artery was 100%, 100%, 85%, which were significantly higher than study group (P < 0.05) . Conclus ions When 2.5 > serum free β-HCGMo M of pregnant women > 2.0, the newborn may be associated with intrauterine retardation. Pregnant women serum freeβ-HCGMo M≥2.5, there is obviously increase in the incidence of neonatal intrauterine retardation. For those withintrauterine retardation, the placenta secondary and tertiary villi artery diameter is small, the number of artery decreases significantly. Three-dimensional power doppler blood flow index of VI, FI and VFI are significantly lower than normal. Serum free β-HCGMoM of pregnant women can predict the placenta function, villi vascular artery hierarchical display rate and the three-dimensional power doppler blood flow index can qualitatively and quantitatively determine the placental function. Placenta in mid gestation will be injured because of ischemia and anoxia, and the placental function can be recovered after placental vascular remodeling.
2017, 38(06): 108-112.
Abstract:
Objective To explore the influence of HSF2 on occurrence of lung cancer and tumor cell proliferation by promoting HSP's expression. Me thods We selected the para-carcinoma tissue from patients with lung cancer, then constructed the eukaryoticvector of HSF2 overexpression, then respectively transfected into BEAS-2B and A549, in order to exam its influence on cell proliferation and HSP expression, and we co-transfected the si RNA of HSP27 and HSP90 while the transfection of p IRES2-EGFP-HSF2, in order to exam the influence on cell proliferation. Re s ults The overexpression of HSF2 may increase BEAS-2B, improve the A549 proliferation speed (P<0.01) and the expressions of HSP27, HSP90. The co-transfected si RNA of HSP27 and HSP90 may equally recede the cell proliferations of BEAS-2B and A549 induced by HSF2 (P <0.01) .Conclus ion The cell proliferation of lung cancer promoted by HSF2 may possibly be achieved through promoting the expression of HSP.
Objective To explore the influence of HSF2 on occurrence of lung cancer and tumor cell proliferation by promoting HSP's expression. Me thods We selected the para-carcinoma tissue from patients with lung cancer, then constructed the eukaryoticvector of HSF2 overexpression, then respectively transfected into BEAS-2B and A549, in order to exam its influence on cell proliferation and HSP expression, and we co-transfected the si RNA of HSP27 and HSP90 while the transfection of p IRES2-EGFP-HSF2, in order to exam the influence on cell proliferation. Re s ults The overexpression of HSF2 may increase BEAS-2B, improve the A549 proliferation speed (P<0.01) and the expressions of HSP27, HSP90. The co-transfected si RNA of HSP27 and HSP90 may equally recede the cell proliferations of BEAS-2B and A549 induced by HSF2 (P <0.01) .Conclus ion The cell proliferation of lung cancer promoted by HSF2 may possibly be achieved through promoting the expression of HSP.
2017, 38(06): 113-117.
Abstract:
Objective To analyze the early clinical outcome of Zero-P VA during the operation of anterior cervical discectomy and fusion ( ACDF) in the treatment of cervical spondylotic myelopathy retrospectively.Me thods A total of 29 patients (33 Zero-P VA has been placed) with cervical spondylotic myelopathy were treated with ACDF with the Zero-P VA from June 2013 to June 2015. The clinical results were evaluated with operation time and bleeding volume, the Bazaz score, the cervical curvature (Cobb angle) , the Visual Analogue Scale ( VAS) , the Japanese Orthopaedic Association ( JOA) scores and the Neck Disability Index (NDI) .Re s ults All patients were followed up for 12 to 24 (average 14.48 ±3.51) months. During the follow-up, no patient was lost to follow-up and no data was lost. The mean operating time was (84.59 ±12.14) minutes. The intra-operative bleeding volume was (19.76 ±6.53) ml, and no postoperative patients developed complications like dysphagia. At the last follow-up, the Cobb angles were improved (P <0.05) , the VAS scales decreased (P<0.05) , average JOA scores significantly increased (P<0.05) , and the NDI scores decreased (P<0.05) .Conclus ion The Zero-P VA has satisfactory short-term clinical result for the treatment of cervical spondylotic myelopathy.
Objective To analyze the early clinical outcome of Zero-P VA during the operation of anterior cervical discectomy and fusion ( ACDF) in the treatment of cervical spondylotic myelopathy retrospectively.Me thods A total of 29 patients (33 Zero-P VA has been placed) with cervical spondylotic myelopathy were treated with ACDF with the Zero-P VA from June 2013 to June 2015. The clinical results were evaluated with operation time and bleeding volume, the Bazaz score, the cervical curvature (Cobb angle) , the Visual Analogue Scale ( VAS) , the Japanese Orthopaedic Association ( JOA) scores and the Neck Disability Index (NDI) .Re s ults All patients were followed up for 12 to 24 (average 14.48 ±3.51) months. During the follow-up, no patient was lost to follow-up and no data was lost. The mean operating time was (84.59 ±12.14) minutes. The intra-operative bleeding volume was (19.76 ±6.53) ml, and no postoperative patients developed complications like dysphagia. At the last follow-up, the Cobb angles were improved (P <0.05) , the VAS scales decreased (P<0.05) , average JOA scores significantly increased (P<0.05) , and the NDI scores decreased (P<0.05) .Conclus ion The Zero-P VA has satisfactory short-term clinical result for the treatment of cervical spondylotic myelopathy.
2017, 38(06): 118-123.
Abstract:
Objective To evaluate the clinical efficacy and adverse effects induced by rh-endostatin (rh-ES) combined with chemotherapy in the treatment of advanced breast cancer. Methods The Pubmed, Embase, Cochrane Library, Spriner, CNKI, VIP, Wangfang Data, CBM were searched for literature up to April 2016. We searched randomized controlled clinical trials of rh-endostatin combined with chemotherapy in the treatment of advanced breast cancer and judged whether the trials met the inclusive criteria. Then Meta analysis was performed by using Rev Man v5.3 software after extracting useful data. Re s ults Six researches with 346 patients were included according to the inclusive criteria. Meta analysis demonstrated that rh-endostatin combined with chemotherapy had statistically significant increase over chemotherapy alone in terms of objective respond rate (RR=1.41, 95% CI: 1.13-1.77) or disease control rate (RR=1.21, 95% CI: 1.08-1.35) , and had statistically significant benefit in progression of disease ( RR=0.49, 95% CI: 0.32-0.75) . The incidence rates of leukopenia, thrombocytopenia and gastrointestinal reaction were no significant difference between two groups.Conclus ions Rh-endostatin combined with chemotherapy may have more meaningful clinical improvement in objective respond rate, disease control rate and progression of disease. The combination therapy had not added the adverse effects rates significantly.
Objective To evaluate the clinical efficacy and adverse effects induced by rh-endostatin (rh-ES) combined with chemotherapy in the treatment of advanced breast cancer. Methods The Pubmed, Embase, Cochrane Library, Spriner, CNKI, VIP, Wangfang Data, CBM were searched for literature up to April 2016. We searched randomized controlled clinical trials of rh-endostatin combined with chemotherapy in the treatment of advanced breast cancer and judged whether the trials met the inclusive criteria. Then Meta analysis was performed by using Rev Man v5.3 software after extracting useful data. Re s ults Six researches with 346 patients were included according to the inclusive criteria. Meta analysis demonstrated that rh-endostatin combined with chemotherapy had statistically significant increase over chemotherapy alone in terms of objective respond rate (RR=1.41, 95% CI: 1.13-1.77) or disease control rate (RR=1.21, 95% CI: 1.08-1.35) , and had statistically significant benefit in progression of disease ( RR=0.49, 95% CI: 0.32-0.75) . The incidence rates of leukopenia, thrombocytopenia and gastrointestinal reaction were no significant difference between two groups.Conclus ions Rh-endostatin combined with chemotherapy may have more meaningful clinical improvement in objective respond rate, disease control rate and progression of disease. The combination therapy had not added the adverse effects rates significantly.
2017, 38(06): 124-129.
Abstract:
Objective To explore the clinical application value of CT plus MRI in the staging diagnosis of endometrial cancer, and to evaluate the malignancy of cancer according to the postoperative level of VEGF-C.Me thods A total of 70 pathologically conformed endometrial cancercases were randomly selected. All patients underwent CT, MRI, and CT plus MRI examinations. Based on the staging confirmed by surgery, the accuracy rates of diagnosis of CT, MRI, and CT plus MRI were compared. The level of postoperative VGEF-C was observed to determine the malignancy of the cancer.Re s ults The accuracy rate of diagnosis of CT, MRI, and CT plus MRI before surgery was 59.4%, 65.7% and 92.1%, respectively. The rate of CT plus MRI was significantly higher than the rate of the other two examinations (P<0.05) . In addition, There was significant difference in the level of VEGF-C between endometrial cancers of differentstages. A positive correlation was observed between preoperative staging determined by CT plus MRI examination and the level of VEGF-C. Conclus ion CT plus MRI examination can produce high accuracy rate of staging diagnosis, which is positively correlated with the postoperative level of VEGF-C. Therefore, CT plus MRI examinationcan predict the malignancy of endometrial cancer, which is helpful for the clinical assessment and prognosis of endometrial cancer.
Objective To explore the clinical application value of CT plus MRI in the staging diagnosis of endometrial cancer, and to evaluate the malignancy of cancer according to the postoperative level of VEGF-C.Me thods A total of 70 pathologically conformed endometrial cancercases were randomly selected. All patients underwent CT, MRI, and CT plus MRI examinations. Based on the staging confirmed by surgery, the accuracy rates of diagnosis of CT, MRI, and CT plus MRI were compared. The level of postoperative VGEF-C was observed to determine the malignancy of the cancer.Re s ults The accuracy rate of diagnosis of CT, MRI, and CT plus MRI before surgery was 59.4%, 65.7% and 92.1%, respectively. The rate of CT plus MRI was significantly higher than the rate of the other two examinations (P<0.05) . In addition, There was significant difference in the level of VEGF-C between endometrial cancers of differentstages. A positive correlation was observed between preoperative staging determined by CT plus MRI examination and the level of VEGF-C. Conclus ion CT plus MRI examination can produce high accuracy rate of staging diagnosis, which is positively correlated with the postoperative level of VEGF-C. Therefore, CT plus MRI examinationcan predict the malignancy of endometrial cancer, which is helpful for the clinical assessment and prognosis of endometrial cancer.
2017, 38(06): 130-135.
Abstract:
Objective To compare the clinical application of atrial active leads and passive leads, and to investigate the feasibility and safety of atrial active electrode lead. Me thods 1 027 patients with pacemaker implantation in Yuxi People's Hospital from January 2002 to March 2016 were selected. According to the patients' choice intention, 530 cases of atrial active electrodes were selected as active electrode group (n = 530) , and there were passive atrial electrodes in the passive electrode group (n = 497) . Then we record atrial electrode operative time, threshold fall time; followed up and recorded the threshold and impedance of the two groups immediately, 1week, 1 month, 3 months and 6 months after implantation. Bed time, average length of stay, comfort score and the rate of dislocation, atrial perforation, capsular infection, new atrial fibrillation were compared.Re s ults (1) Atrial active electrode group implanted atrial electrode time was shorter than control group. (2) The threshold fall time of atrial active electrode group was 4.38±2.62 minutes. (3) In pacing parameters, the threshold value of active electrode implantation was higher than that of passive electrode group while impedance was lower than that of passive electrode group on opration;After operation, the parameters of active electrode group were stable for a long time. (4) Pneumothorax and myocardial perforation had no significant difference between the two groups. (5) The incidence of electrode dislocation, urinary retention and intestinal paralysis in active electrode group was significantly lower than that in passive electrode group. (6) In the follow-up of active electrode group, the incidence of new atrial fibrillation and atrial tachycardia decreased. (7) After implantation of active electrode, patients' bed time was reduced, the average length of stay was shortened and patient's feeling was improved.Conclus ion The application of atrial active electrode is safe and feasible, and deserves promoton.
Objective To compare the clinical application of atrial active leads and passive leads, and to investigate the feasibility and safety of atrial active electrode lead. Me thods 1 027 patients with pacemaker implantation in Yuxi People's Hospital from January 2002 to March 2016 were selected. According to the patients' choice intention, 530 cases of atrial active electrodes were selected as active electrode group (n = 530) , and there were passive atrial electrodes in the passive electrode group (n = 497) . Then we record atrial electrode operative time, threshold fall time; followed up and recorded the threshold and impedance of the two groups immediately, 1week, 1 month, 3 months and 6 months after implantation. Bed time, average length of stay, comfort score and the rate of dislocation, atrial perforation, capsular infection, new atrial fibrillation were compared.Re s ults (1) Atrial active electrode group implanted atrial electrode time was shorter than control group. (2) The threshold fall time of atrial active electrode group was 4.38±2.62 minutes. (3) In pacing parameters, the threshold value of active electrode implantation was higher than that of passive electrode group while impedance was lower than that of passive electrode group on opration;After operation, the parameters of active electrode group were stable for a long time. (4) Pneumothorax and myocardial perforation had no significant difference between the two groups. (5) The incidence of electrode dislocation, urinary retention and intestinal paralysis in active electrode group was significantly lower than that in passive electrode group. (6) In the follow-up of active electrode group, the incidence of new atrial fibrillation and atrial tachycardia decreased. (7) After implantation of active electrode, patients' bed time was reduced, the average length of stay was shortened and patient's feeling was improved.Conclus ion The application of atrial active electrode is safe and feasible, and deserves promoton.
2017, 38(06): 136-139.
Abstract:
Objective To investigate the correlation between liver tissue inflammation and fibrosis degrees and CD4+ T-lymphocytes level in the patients with HCV/HIV co-infection. Me thods The relationship between liver biopsy histological inflammatory grade and fibrosis stage, liver function (ALT, AST, TBIL and ALB) , CD4+ T-lymphocytes level of 50 cases HCV/HIV co-infection patients was analyzed. Re s ults There were 47 cases (94%) with liver tissue inflammatory grade G2, and 45 cases (90%) for fibrosis stage S2. Whether liver function was normal or not, most of the patients presented with severe liver tissue inflammatory and fibrosis. There were no distinct differences in serum levels of ALT, AST, TBIL and ALB between inflammatory grade and fibrosis stage (P>0.05) . There were distinct differences on inflammatory grade and fibrosis stage between CD4≤200 cell/mm3 and CD4 ≤200 cell/mm3 group (P <0.05) . Conclus ions Liver biopsy is indispensable for confirming liver inflammatory staging and fibrotic scoring grading. Most of the patients with HCV/HIV co-infection present needing anti-HCV therapy. The CD4+T-lymphocytes level of patients could be a sensitive index for the estimation of the severity of liver damage.
Objective To investigate the correlation between liver tissue inflammation and fibrosis degrees and CD4+ T-lymphocytes level in the patients with HCV/HIV co-infection. Me thods The relationship between liver biopsy histological inflammatory grade and fibrosis stage, liver function (ALT, AST, TBIL and ALB) , CD4+ T-lymphocytes level of 50 cases HCV/HIV co-infection patients was analyzed. Re s ults There were 47 cases (94%) with liver tissue inflammatory grade G2, and 45 cases (90%) for fibrosis stage S2. Whether liver function was normal or not, most of the patients presented with severe liver tissue inflammatory and fibrosis. There were no distinct differences in serum levels of ALT, AST, TBIL and ALB between inflammatory grade and fibrosis stage (P>0.05) . There were distinct differences on inflammatory grade and fibrosis stage between CD4≤200 cell/mm3 and CD4 ≤200 cell/mm3 group (P <0.05) . Conclus ions Liver biopsy is indispensable for confirming liver inflammatory staging and fibrotic scoring grading. Most of the patients with HCV/HIV co-infection present needing anti-HCV therapy. The CD4+T-lymphocytes level of patients could be a sensitive index for the estimation of the severity of liver damage.
2017, 38(06): 140-143.
Abstract:
Nursing quality is closely related to the curative effect, treatment compliance, treatment cost, quality of life and recurrence rate of patients with peptic ulcer. In order to summarize the recent progress in the research and practice of peptic ulcer nursing, the two aspects of conventional nursing and nursing model were reviewed.
Nursing quality is closely related to the curative effect, treatment compliance, treatment cost, quality of life and recurrence rate of patients with peptic ulcer. In order to summarize the recent progress in the research and practice of peptic ulcer nursing, the two aspects of conventional nursing and nursing model were reviewed.
2017, 38(06): 144-146.
Abstract:
Objective In order to adapt to the requirements of teaching reform and to stimulate students' interest, Combination of PBL, CBL and TBL teaching method is introduced to the anesthetic pharmacology experiment, and its teaching effect is discussed. Me thods 96 undergraduate students in class 1 and class 2 of2014 grade anesthesiology professional in Kunming medical university were selected as the object, and randomly divided into Combination of PBL, CBL and TBL teaching group and traditional teaching group. Then we compared the teaching effect between two groups through the evaluation of the experiment report and theory test. Re s ults Compared with traditional teaching group, the scores of the experiment report and test paper in Combination of PBL, CBL and TBL teaching method group were significantly different (P <0.05) . Conclus ion The effect of combination of PBL, CBL and TBL teaching method is better than traditional teaching method in anesthetic pharmacology experiment teaching.
Objective In order to adapt to the requirements of teaching reform and to stimulate students' interest, Combination of PBL, CBL and TBL teaching method is introduced to the anesthetic pharmacology experiment, and its teaching effect is discussed. Me thods 96 undergraduate students in class 1 and class 2 of2014 grade anesthesiology professional in Kunming medical university were selected as the object, and randomly divided into Combination of PBL, CBL and TBL teaching group and traditional teaching group. Then we compared the teaching effect between two groups through the evaluation of the experiment report and theory test. Re s ults Compared with traditional teaching group, the scores of the experiment report and test paper in Combination of PBL, CBL and TBL teaching method group were significantly different (P <0.05) . Conclus ion The effect of combination of PBL, CBL and TBL teaching method is better than traditional teaching method in anesthetic pharmacology experiment teaching.
2017, 38(06): 147-150.
Abstract:
Objective To explore the application effect of U fixation in neonatal needle indwelling. Methods318 cases of newborns with needle indwelling for intravenous infusion in the department of pediatrics in the third people's hospital in Yunnan Province in May 2016-February 2016, were randomly divided into experimental group and control group, with 159 cases in each group. The experimental group was given U fixation while the control group was given ordinary fixation (straight fixation) . Then we compared the application effect of two groups.Re s ults There were statistically significant differences in the accidental removal of indwelling needle, tube plugging by needle, returning blood of indwelling needle and needle indwelling time between experimental group and control group (P < 0.05) . Conclus ions In neonatal needle indwelling process, U fixation can greatly reduce the incidence of accidental removal of indwelling needle, tube plugging by needle, returning blood of indwelling needle and extend the needle indwelling time. This method is simple, more firmly fixed, and the extention tube is U-shaped, is very useful in clinical work, and can reduce venous thrombosis, the potential risk of nosocomial infection, so it is suitable for popularization and application in clinical.
Objective To explore the application effect of U fixation in neonatal needle indwelling. Methods318 cases of newborns with needle indwelling for intravenous infusion in the department of pediatrics in the third people's hospital in Yunnan Province in May 2016-February 2016, were randomly divided into experimental group and control group, with 159 cases in each group. The experimental group was given U fixation while the control group was given ordinary fixation (straight fixation) . Then we compared the application effect of two groups.Re s ults There were statistically significant differences in the accidental removal of indwelling needle, tube plugging by needle, returning blood of indwelling needle and needle indwelling time between experimental group and control group (P < 0.05) . Conclus ions In neonatal needle indwelling process, U fixation can greatly reduce the incidence of accidental removal of indwelling needle, tube plugging by needle, returning blood of indwelling needle and extend the needle indwelling time. This method is simple, more firmly fixed, and the extention tube is U-shaped, is very useful in clinical work, and can reduce venous thrombosis, the potential risk of nosocomial infection, so it is suitable for popularization and application in clinical.
