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RSS2018 Vol. 39, No. 03
2018, 39(03): 1-4.
Abstract:
Objective To study the chemical constituents from Bletilla ochracea. Methods The compounds were extracted by 95% alcohol and isolated by column chromatography on silica gel and Sephadex LH-20. Their structures were identified by spectroscopic analysis (~1 H NMR and 13 CNMR) .Results Nine compouds were obtained and identified as lusianthridin (1) , 1, 2, 7-trihydroxy-4-methoxy-9, 10-dihydroxyphenanthrene (2) , nudol (3) , coelonin (4) , batatasin Ⅲ (5) , 3, 7-dihydroxy-2, 4-dimethoxyphenanthrene (6) , daucosterol (7) , β-sitosterol (8) , stigmasterol (9) .Conclus ion Compounds 2, 3, 5 were isolated from this plant for the first time.
Objective To study the chemical constituents from Bletilla ochracea. Methods The compounds were extracted by 95% alcohol and isolated by column chromatography on silica gel and Sephadex LH-20. Their structures were identified by spectroscopic analysis (~1 H NMR and 13 CNMR) .Results Nine compouds were obtained and identified as lusianthridin (1) , 1, 2, 7-trihydroxy-4-methoxy-9, 10-dihydroxyphenanthrene (2) , nudol (3) , coelonin (4) , batatasin Ⅲ (5) , 3, 7-dihydroxy-2, 4-dimethoxyphenanthrene (6) , daucosterol (7) , β-sitosterol (8) , stigmasterol (9) .Conclus ion Compounds 2, 3, 5 were isolated from this plant for the first time.
2018, 39(03): 5-10.
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Objective To study the effect of OK on the differentiation of bone marrow stromal stem cells (BMSCs) through the TGFβ/Smad signaling pathway in rats. Methods We removed the bilateral ovaries of rats to replicate OP model, and isolated and cultured BMSCs. BMSCs isolated from nomal rats were cultured as control group, BMSCs isolated from OP rats were divided into 5 groups, OP model group was regularly cultured, positive control group was treated with alendronate sodium (1 μmol/L) , low, medium and high OK treatment group were treated with 50 mL/L, 100 mL/L and 200 ml/L OK respectively.After 24 h incubation, all cells were collected. The proliferation rate of BMSCs was determined by MTT method, and ELISA method was employed to detect the contents of bone formation markers, RT-qPCR was used to determine the m RNA expression level of TGFβ and the protein expression levels of TGFβ, p-Smad2/3 and of Smad2/3 were detected by Western blot. Results Compared with control group, the proliferation rate of the BMSCs in OP model group was reduced, concentrations of bone formation markers (OC, PINP and BALP) were reduced, m RNA and protein expression levels of TGFβ, as well as the phosphorylated level of Smad2/3 were downregulated.The difference was statistically significant (P<0.05) . After treatment with OK, compared with model group, all the above effects were ameliorated in different degree, a dose dependent manner was observed in OK treatment group, and the treatment effects of alendronate sodium (1 μmol/L) , 100 mL/L and 200 mL/L OK group were statistically significant (P <0.05) .Conclusion OK can promote the proliferation of osteoblasts by activating TGFβ/Smad signaling pathway to achieve the effect of treating postmenopausal OP.
Objective To study the effect of OK on the differentiation of bone marrow stromal stem cells (BMSCs) through the TGFβ/Smad signaling pathway in rats. Methods We removed the bilateral ovaries of rats to replicate OP model, and isolated and cultured BMSCs. BMSCs isolated from nomal rats were cultured as control group, BMSCs isolated from OP rats were divided into 5 groups, OP model group was regularly cultured, positive control group was treated with alendronate sodium (1 μmol/L) , low, medium and high OK treatment group were treated with 50 mL/L, 100 mL/L and 200 ml/L OK respectively.After 24 h incubation, all cells were collected. The proliferation rate of BMSCs was determined by MTT method, and ELISA method was employed to detect the contents of bone formation markers, RT-qPCR was used to determine the m RNA expression level of TGFβ and the protein expression levels of TGFβ, p-Smad2/3 and of Smad2/3 were detected by Western blot. Results Compared with control group, the proliferation rate of the BMSCs in OP model group was reduced, concentrations of bone formation markers (OC, PINP and BALP) were reduced, m RNA and protein expression levels of TGFβ, as well as the phosphorylated level of Smad2/3 were downregulated.The difference was statistically significant (P<0.05) . After treatment with OK, compared with model group, all the above effects were ameliorated in different degree, a dose dependent manner was observed in OK treatment group, and the treatment effects of alendronate sodium (1 μmol/L) , 100 mL/L and 200 mL/L OK group were statistically significant (P <0.05) .Conclusion OK can promote the proliferation of osteoblasts by activating TGFβ/Smad signaling pathway to achieve the effect of treating postmenopausal OP.
2018, 39(03): 11-18.
Abstract:
Objective To reconstruct the deformity of appearance and function of patients with bone defect, co-cultured system with two stem cells were combined with partial deproteinized biological bone to reconstruct the defect of tibia which is one of the main weight-bearing bone. Methods The bone marrow and peripheral blood were harvested form 18 New Zealand rabbits to isolated bone marrow stem cells and epithelial progenitor cells, and engineering bone was constructed with co-cultured system with these two stem cells and partial deproteinized biological bones; about 1 CM of bone defect of each rabbit was made with bone rongeur, then engineering bones were transplanted into the defect area, the osteogenesis and bone defect recovery were observed on day 14, 28 and month 2.Results The difference of absorbance values of BMSCs group, co-cultured cell group and blank group at each time point and between groups were all statistically significant (P<0.001) , and the collagen content of bone tissue increased gradually after implantation of tissue engineered bone, and the difference between each group was statistically significant (P <0.001) . The repairment of bone defect with the PDPBB combined with BMSCs and EPCs system has the strongest ability to repair the structure andfunction of the tibial defect area. Conclusion The engineering bone constructed with two stem cells and partial deproteinized bone is a good material for bone defect reconstruction.
Objective To reconstruct the deformity of appearance and function of patients with bone defect, co-cultured system with two stem cells were combined with partial deproteinized biological bone to reconstruct the defect of tibia which is one of the main weight-bearing bone. Methods The bone marrow and peripheral blood were harvested form 18 New Zealand rabbits to isolated bone marrow stem cells and epithelial progenitor cells, and engineering bone was constructed with co-cultured system with these two stem cells and partial deproteinized biological bones; about 1 CM of bone defect of each rabbit was made with bone rongeur, then engineering bones were transplanted into the defect area, the osteogenesis and bone defect recovery were observed on day 14, 28 and month 2.Results The difference of absorbance values of BMSCs group, co-cultured cell group and blank group at each time point and between groups were all statistically significant (P<0.001) , and the collagen content of bone tissue increased gradually after implantation of tissue engineered bone, and the difference between each group was statistically significant (P <0.001) . The repairment of bone defect with the PDPBB combined with BMSCs and EPCs system has the strongest ability to repair the structure andfunction of the tibial defect area. Conclusion The engineering bone constructed with two stem cells and partial deproteinized bone is a good material for bone defect reconstruction.
2018, 39(03): 19-22.
Abstract:
Objective To establish the orthotopic model of Cal27 tongue squamous carcinoma cells.Me thods 2×106 Cal27 cells suspension was injected into floor of mouth of nude mice. Then the tumor volume was measured periodically and the body weight of the nude mice was recorded. 14 days later, nude mice were killed, and the primary tumor and adjacent tissues, lungs, and liver were harvested. The tissue sections were stained with hematoxylin and eosin. The submandibular lymph nodes and lung, liver metastasis, and statistical metastasis rate were observed. Re s ults The tumor formed after day 3 that nude mice were inoculated with Cal27 cells, and tumor formation rate was 100% (24/24) . HE staining showed tumor tissue had typical performance of squamous carcinom. The squamous carcinoma cells were found in the submandibular lymph nodes, and the metastasis rate was 70% (17/24) . There were no metastasis found in liver and lung. Conclus ions Orthotopic metastatic model of oral cancer in nude mice has been established successfully. This animal model can objectively reflect the biological behavior of human oral cancer, is one of the ideal methods to study the invasion and metastasis of oral squamous cell carcinoma.
Objective To establish the orthotopic model of Cal27 tongue squamous carcinoma cells.Me thods 2×106 Cal27 cells suspension was injected into floor of mouth of nude mice. Then the tumor volume was measured periodically and the body weight of the nude mice was recorded. 14 days later, nude mice were killed, and the primary tumor and adjacent tissues, lungs, and liver were harvested. The tissue sections were stained with hematoxylin and eosin. The submandibular lymph nodes and lung, liver metastasis, and statistical metastasis rate were observed. Re s ults The tumor formed after day 3 that nude mice were inoculated with Cal27 cells, and tumor formation rate was 100% (24/24) . HE staining showed tumor tissue had typical performance of squamous carcinom. The squamous carcinoma cells were found in the submandibular lymph nodes, and the metastasis rate was 70% (17/24) . There were no metastasis found in liver and lung. Conclus ions Orthotopic metastatic model of oral cancer in nude mice has been established successfully. This animal model can objectively reflect the biological behavior of human oral cancer, is one of the ideal methods to study the invasion and metastasis of oral squamous cell carcinoma.
2018, 39(03): 23-30.
Abstract:
Objective To prepare pH sensitive gel for eye cornea of salvianolic acid B and accomplish in vitro release study. Me thods We used corneal permeability, eye irritation in rabbits, hydration of the cornea, viscosity, gel capacity, transparency, liquidity as index, to select pH sensitive gel matrix composition. Re s ults The gel composition was: 0.5% of salvianolic acid B, 0.14% Carbomer, 0.3% HPMC, 0.05% Azone, 0.1% ethylparaben and 0.2% Vc. The corneal hydration level was 77.32±1.88.The gel released 27.21% drug in 15 min, and released 97.44% drug in 6 h. By research of corneal permeability, the cumulative release of gel was bigger than the one of saline (P<0.05) . Conclus ions The selection of the gel matrix prescription is reasonable in design, simple in process, not harsh to eyes, and can prolong the retention time of drug in corneas and improve the corneal penetration.
Objective To prepare pH sensitive gel for eye cornea of salvianolic acid B and accomplish in vitro release study. Me thods We used corneal permeability, eye irritation in rabbits, hydration of the cornea, viscosity, gel capacity, transparency, liquidity as index, to select pH sensitive gel matrix composition. Re s ults The gel composition was: 0.5% of salvianolic acid B, 0.14% Carbomer, 0.3% HPMC, 0.05% Azone, 0.1% ethylparaben and 0.2% Vc. The corneal hydration level was 77.32±1.88.The gel released 27.21% drug in 15 min, and released 97.44% drug in 6 h. By research of corneal permeability, the cumulative release of gel was bigger than the one of saline (P<0.05) . Conclus ions The selection of the gel matrix prescription is reasonable in design, simple in process, not harsh to eyes, and can prolong the retention time of drug in corneas and improve the corneal penetration.
2018, 39(03): 31-37.
Abstract:
Objective To investigate the effect of joint therapy by NT-3-HUMSCs and SOCS3 gene silencing in promoting the injury nerve regeneration repair after spinal cord injury in SD rats. Methods (1) Adherence method was used to culture human umbilical cord-derived mesenchymal cells (HUMSC) in vitro for separation, purification and identification. (2) We constructed NT-3 gene eukaryotic expression vector, and used gene transfection technology into its HUMSC, and tested the survival of NT-3-HUMSC cells and NT-3 expression in cells. (3) We screened specific targets of SOCS3, made sequence homology analysis, and set a negative control, designed and synthesized siRNA and detected the function. (4) SD rats model of spinal cordinjury were established and divided into: 1. sham group 10; 2.T12 whole spinal cord injury model 40, were randomly divided into four groups, respectively; saline treatment group 10; siRNA + NT-3-HUMSCs treatment group 10; NT-3-HUMSCs treatment group 10; siRNA treated group 10. After each group above modeling success, they received respectively the neural electrophysiological monitoring for 12 weeks survival. (5) We perfused SD rats for fixation and collect samples, and observed the local glial scar degradation situation and axon regeneration, meanwhile, used biotin glucan fluorescent (BDA) anterograde tracing. The injury transplant area-host junction spinal cord tissues were collected to observe the corticospinal tract regeneration under microscope. Results (1) In siRNA + NT-3-HUMSCs treatment group, the transection syringomyelia was significantly reduced as compared with normal saline group (P < 0.05) . (2) BDA anterograde tracing results showed that in the siRNA + NT-3-HUMSCs treatment group, neural axon grew significantly compared with the normal saline group. (3) Neural electrophysiological testing 12 weeks after injury: in the treatment group, the incubation period P40 was shorter as compared with control group; in siRNA + NT-3-HUMSCs treatment group, the incubation period was shorter obviously than normal saline, but the amplitude increased obviously (P < 0.05) . Conclusion NT-3-HUMSCs joint with SOCS3 gene silencing can promote the injury nerve regeneration repair in the treatment of SD rat spinal cord injury.
Objective To investigate the effect of joint therapy by NT-3-HUMSCs and SOCS3 gene silencing in promoting the injury nerve regeneration repair after spinal cord injury in SD rats. Methods (1) Adherence method was used to culture human umbilical cord-derived mesenchymal cells (HUMSC) in vitro for separation, purification and identification. (2) We constructed NT-3 gene eukaryotic expression vector, and used gene transfection technology into its HUMSC, and tested the survival of NT-3-HUMSC cells and NT-3 expression in cells. (3) We screened specific targets of SOCS3, made sequence homology analysis, and set a negative control, designed and synthesized siRNA and detected the function. (4) SD rats model of spinal cordinjury were established and divided into: 1. sham group 10; 2.T12 whole spinal cord injury model 40, were randomly divided into four groups, respectively; saline treatment group 10; siRNA + NT-3-HUMSCs treatment group 10; NT-3-HUMSCs treatment group 10; siRNA treated group 10. After each group above modeling success, they received respectively the neural electrophysiological monitoring for 12 weeks survival. (5) We perfused SD rats for fixation and collect samples, and observed the local glial scar degradation situation and axon regeneration, meanwhile, used biotin glucan fluorescent (BDA) anterograde tracing. The injury transplant area-host junction spinal cord tissues were collected to observe the corticospinal tract regeneration under microscope. Results (1) In siRNA + NT-3-HUMSCs treatment group, the transection syringomyelia was significantly reduced as compared with normal saline group (P < 0.05) . (2) BDA anterograde tracing results showed that in the siRNA + NT-3-HUMSCs treatment group, neural axon grew significantly compared with the normal saline group. (3) Neural electrophysiological testing 12 weeks after injury: in the treatment group, the incubation period P40 was shorter as compared with control group; in siRNA + NT-3-HUMSCs treatment group, the incubation period was shorter obviously than normal saline, but the amplitude increased obviously (P < 0.05) . Conclusion NT-3-HUMSCs joint with SOCS3 gene silencing can promote the injury nerve regeneration repair in the treatment of SD rat spinal cord injury.
2018, 39(03): 38-41.
Abstract:
Objective To make a parallel mining the data of expression differences of a crucial gene XPA involved in nucleotide excision repair pathway of human skin microarrays by bioinformatics from the system level.Methods Using the ScanGEO, the data of microarrays which included the significant differences expression level of XPA were screened and analyzed from 59 human skin samples in the GEO database. Results There were 7 samples with the down-regulated expression of XPA: cutaneous malignant melanoma, epidermal injury model, DNA damage and UV radiation, foreskin fibroblast response to Toxoplasma gondii RH type 1 (ROP5) mutant infection, interleukin-20 subfamily cytokines effect on epidermal keratinocytes, Egr-1 overexpression effect on skin fibroblasts in vitro: time course, in vitro model for inflammatory dendritic cells.Present expression down. Conclusion Based on the GEO database and ScanGEO, high-throughput shared data can be screened and analyzed efficiently.
Objective To make a parallel mining the data of expression differences of a crucial gene XPA involved in nucleotide excision repair pathway of human skin microarrays by bioinformatics from the system level.Methods Using the ScanGEO, the data of microarrays which included the significant differences expression level of XPA were screened and analyzed from 59 human skin samples in the GEO database. Results There were 7 samples with the down-regulated expression of XPA: cutaneous malignant melanoma, epidermal injury model, DNA damage and UV radiation, foreskin fibroblast response to Toxoplasma gondii RH type 1 (ROP5) mutant infection, interleukin-20 subfamily cytokines effect on epidermal keratinocytes, Egr-1 overexpression effect on skin fibroblasts in vitro: time course, in vitro model for inflammatory dendritic cells.Present expression down. Conclusion Based on the GEO database and ScanGEO, high-throughput shared data can be screened and analyzed efficiently.
2018, 39(03): 42-48.
Abstract:
Objective To provide theoretical basis for the rational use of drugs in clinic, the biochemical characteristics of liver injury induced by Atorvastatin Combined with HRZ (Isoniazid Rifampicin and Pyrazinamide) were analyzed in animal model. Me thods Eighty 8 week old SPF SD rats with half males and females were divided into four groups: control group, atorvastatin group, HRZ group, atorvastatin+HRZ group. According to the human mouse drug dose conversion, mice were given corresponding drugs by gavage.Hepatic function index of rats (the Total bilirubin, Direct bilirubin, Indirect bilirubin, Aspartate aminotransferase, Alanine aminotransferase, Alkaline phosphatase) were detected by blood from the femoral artery and hepatic function index of rats in each group on 10 d, 20 d, 40 d.Re s ults There were significant difference in the anmmistrated group on 10, 20, 40 days with higher TBIL, DBIL, IBIL than that in control group; in the admimistrated group on Day 10, combined treatment group was higher than that in cotrol group and there were significant differences in ALT;in the process of treatmen, there was statistical difference; ALP was administered for 20 days and the 40 day, atorvastatin there was HRZ group was statistically different in groupHRZ, severe injury, combination group compared with HRZ group had statistically significant difference. Conclus ions The liver injury in the three experimental groups is mild and moderate, and the liver damage is mainly cholestasis type. The most severe hepatic injury caused by Atorvastatin Combined with HRZ is aggravated by the prolonged use of drugs.
Objective To provide theoretical basis for the rational use of drugs in clinic, the biochemical characteristics of liver injury induced by Atorvastatin Combined with HRZ (Isoniazid Rifampicin and Pyrazinamide) were analyzed in animal model. Me thods Eighty 8 week old SPF SD rats with half males and females were divided into four groups: control group, atorvastatin group, HRZ group, atorvastatin+HRZ group. According to the human mouse drug dose conversion, mice were given corresponding drugs by gavage.Hepatic function index of rats (the Total bilirubin, Direct bilirubin, Indirect bilirubin, Aspartate aminotransferase, Alanine aminotransferase, Alkaline phosphatase) were detected by blood from the femoral artery and hepatic function index of rats in each group on 10 d, 20 d, 40 d.Re s ults There were significant difference in the anmmistrated group on 10, 20, 40 days with higher TBIL, DBIL, IBIL than that in control group; in the admimistrated group on Day 10, combined treatment group was higher than that in cotrol group and there were significant differences in ALT;in the process of treatmen, there was statistical difference; ALP was administered for 20 days and the 40 day, atorvastatin there was HRZ group was statistically different in groupHRZ, severe injury, combination group compared with HRZ group had statistically significant difference. Conclus ions The liver injury in the three experimental groups is mild and moderate, and the liver damage is mainly cholestasis type. The most severe hepatic injury caused by Atorvastatin Combined with HRZ is aggravated by the prolonged use of drugs.
2018, 39(03): 55-58.
Abstract:
Objective To analyze the property of the new antibacterial agent (N, N-di-n-decyl-N, N-dimethyl-ammonium 5-oxopyrrolidine-2-carboxylate, DAPC) to prevent Streptococcus mutans' adhesion on tooth surface.Method determine the minimal inhibitory concentration and minimal bactericidal concentration of DAPC and Chlorhexidine gluconate by liquid dilution method.Set the Chlorhexidine gluconate as the positive control, while PBS as the negative control.Use the crystal violet staining to measure the quantity of biofilm.Re s ult The MIC of DAPC on Streptococcus mutans was 0.0031250% and MBC was 0.0062500% . The MIC of Chlorhexidine gluconate was 0.0015625% , while the MBC was 0.0031250% .When concentration of the two antibacterial agents was MIC, the quantity of biofilm have no significance among three groups.However, when concentration of Chlorhexidine gluconate and DAPC were 0.12% and 0.24% respectively, biofilm of experimental group was lower than PBS, and there is no significance between Chlorhexidine gluconate group and DAPC group after 24 hours incubation.Conclus ion New antibacterial agent DAPC have significant effect in inhibiting of Streptococcus mutans.And the residual of DAPC on teeth can maintain a long time of antibacterial effect to inhibit Streptococcus mutans' adhesion.
Objective To analyze the property of the new antibacterial agent (N, N-di-n-decyl-N, N-dimethyl-ammonium 5-oxopyrrolidine-2-carboxylate, DAPC) to prevent Streptococcus mutans' adhesion on tooth surface.Method determine the minimal inhibitory concentration and minimal bactericidal concentration of DAPC and Chlorhexidine gluconate by liquid dilution method.Set the Chlorhexidine gluconate as the positive control, while PBS as the negative control.Use the crystal violet staining to measure the quantity of biofilm.Re s ult The MIC of DAPC on Streptococcus mutans was 0.0031250% and MBC was 0.0062500% . The MIC of Chlorhexidine gluconate was 0.0015625% , while the MBC was 0.0031250% .When concentration of the two antibacterial agents was MIC, the quantity of biofilm have no significance among three groups.However, when concentration of Chlorhexidine gluconate and DAPC were 0.12% and 0.24% respectively, biofilm of experimental group was lower than PBS, and there is no significance between Chlorhexidine gluconate group and DAPC group after 24 hours incubation.Conclus ion New antibacterial agent DAPC have significant effect in inhibiting of Streptococcus mutans.And the residual of DAPC on teeth can maintain a long time of antibacterial effect to inhibit Streptococcus mutans' adhesion.
2018, 39(03): 59-61.
Abstract:
Objective To discuss the model and the effects of intervention treatment among patients with cerebral apoplexy during the recovery period in the primary and tertiary hospitals. Methods From Mar. 2015 to Feb. 2016, we selected 119 patients with cerebral apoplexy cured in our department and randomly divided them into two groups: 61 cases in the combined treatment group and 58 cases in the control group. After 6-month treatment, the effects were evaluated using FMA and BI index. Results FMA and BI indexes of patients with cerebral apoplexy improved significantly after the treatment (P <0.05) . The effect of using the combined treatment is better than that of the control group (P<0. 05) . Conclusion The recovery model performed in the primary hospital together with the tertiary hospital is beneficial to the recovery of patients with cerebral apoplexy and worthy to generalize.
Objective To discuss the model and the effects of intervention treatment among patients with cerebral apoplexy during the recovery period in the primary and tertiary hospitals. Methods From Mar. 2015 to Feb. 2016, we selected 119 patients with cerebral apoplexy cured in our department and randomly divided them into two groups: 61 cases in the combined treatment group and 58 cases in the control group. After 6-month treatment, the effects were evaluated using FMA and BI index. Results FMA and BI indexes of patients with cerebral apoplexy improved significantly after the treatment (P <0.05) . The effect of using the combined treatment is better than that of the control group (P<0. 05) . Conclusion The recovery model performed in the primary hospital together with the tertiary hospital is beneficial to the recovery of patients with cerebral apoplexy and worthy to generalize.
2018, 39(03): 67-71.
Abstract:
Objective To explore the family function and simple coping style of parents with cleft lip and palate, and to promote the improvement of their emotional and family problems, and to provide some basis for the prevention of psychological problems in children with cleft lip and palate. Methods A total of 79 parents of cleft lip and cleft palate children under 6 years old were surveyed by means of family function scale and simple coping style scale.Results The was statistical significance in the correlation analysis of family function scale, solve the problem of (r=0.237, P < 0.05) , behavioral control (r=0.267, P < 0.05) . Simple coping style scale correlation analysis of positive coping in age of parents and parents showed significant statistical difference (r=-0.246, P < 0.05) . Conclusion Parents of children with cleft lip and palate have poor ability to deal with emotional reaction and problem solving when stimulating, such as emotional and emotional expression and confession, family members' lack of information communication and language communication, etc, in the face of unexpected events and dangerous situations, the behavior control model is not good, and it is easy to be at a loss; and the older parents are, the fewer positive coping styles they take. The family function and coping style of parents are of great significance to the physical and mental health of children with cleft lip and palate, and psychological intervention should be carried out in time.
Objective To explore the family function and simple coping style of parents with cleft lip and palate, and to promote the improvement of their emotional and family problems, and to provide some basis for the prevention of psychological problems in children with cleft lip and palate. Methods A total of 79 parents of cleft lip and cleft palate children under 6 years old were surveyed by means of family function scale and simple coping style scale.Results The was statistical significance in the correlation analysis of family function scale, solve the problem of (r=0.237, P < 0.05) , behavioral control (r=0.267, P < 0.05) . Simple coping style scale correlation analysis of positive coping in age of parents and parents showed significant statistical difference (r=-0.246, P < 0.05) . Conclusion Parents of children with cleft lip and palate have poor ability to deal with emotional reaction and problem solving when stimulating, such as emotional and emotional expression and confession, family members' lack of information communication and language communication, etc, in the face of unexpected events and dangerous situations, the behavior control model is not good, and it is easy to be at a loss; and the older parents are, the fewer positive coping styles they take. The family function and coping style of parents are of great significance to the physical and mental health of children with cleft lip and palate, and psychological intervention should be carried out in time.
2018, 39(03): 72-76.
Abstract:
Objective To observe the relationship of IL-17 and arterial blood gas, fibrin fragment D (D-D) in patients with both pulmonary embolism (PE) and obstructive sleep apnea-hypopnea syndrome (OSAHS) . Methods We collected 20 patients with both PE and OSAHS and 43 patients with only PE who hospitalized in the first ward of pneumology department of the Second Affiliated Hospital of Kunming Medical University, then measured D-D, IL-17 in venous blood and arterial blood gas and recorded them to analysis.Re s ults PO2 in patients with both PE and OSAHS was significantly lower than that in patients with only PE, while D-D and IL-17 of the latter were markedly lesser than the former. Conclus ions The expression of IL-17 between the 2 groups of patients has statistically significant difference. What's more, the expression of IL-17 is positively associated with hypercoagulability and Body Mass Index (BMI) , and the result shows a negative relation between arterial blood oxygen partial pressure and IL-17, suggesting that IL-17 may be relate to the common progress of PE and OSASH. Thus, IL-17 can be used in the detection of patients with both PE and OSASH
Objective To observe the relationship of IL-17 and arterial blood gas, fibrin fragment D (D-D) in patients with both pulmonary embolism (PE) and obstructive sleep apnea-hypopnea syndrome (OSAHS) . Methods We collected 20 patients with both PE and OSAHS and 43 patients with only PE who hospitalized in the first ward of pneumology department of the Second Affiliated Hospital of Kunming Medical University, then measured D-D, IL-17 in venous blood and arterial blood gas and recorded them to analysis.Re s ults PO2 in patients with both PE and OSAHS was significantly lower than that in patients with only PE, while D-D and IL-17 of the latter were markedly lesser than the former. Conclus ions The expression of IL-17 between the 2 groups of patients has statistically significant difference. What's more, the expression of IL-17 is positively associated with hypercoagulability and Body Mass Index (BMI) , and the result shows a negative relation between arterial blood oxygen partial pressure and IL-17, suggesting that IL-17 may be relate to the common progress of PE and OSASH. Thus, IL-17 can be used in the detection of patients with both PE and OSASH
2018, 39(03): 77-80.
Abstract:
Objective To make epidemiological investigation on children and teenagers with congenital heart disease (CHD) from October 2015 to November 2016 in three counties in Zhaotong area. Methods The examination was divided into the following two phases: primary screening, secondary screening. Standardized echocardiographic examination was performed for the students with suspected CHD. we combined comprehensive analysis of electrocardiogram, X-ray and cardiac catheterization to make a clear diagnosis for patients. The index of prevalence rate of CHD, the types of the disease, and gender of the patients in 14957 children and teenagers were statistically analyzed. Results Altogether 61 cases of CHD were discovered. The total prevalence of CHD was 4.08‰ (61 of 14957 cases) , the prevalence rate in male was 4.55‰, in female was 3.61‰, No difference of the prevalence rate was found between male and female (χ2=0.823, P>0.05) ;the difference among different age groups had no statistical significance (χ2=4.692, P>0.05) ;The prevalence rate of CHD in Qiaojia was 5.17‰, in Daguan was 6.36‰, and in Zhenxiong was 3.03‰, There was no significant difference in the prevalence rate among different countries (χ2=5.897, P>0.05) . In terms of the types of the disease, atrial septal defect accounted for the first (37.7% ) , followed by ventricular septal defect (26.2% ) and patent ductus arteriosus (19.7% ) , others types of CHD were rare. Conclusion The prevalence of CHD in investigated area is slightly higher than the average level of the nationwide. The main types of CHD are atrial septal defect, ventricular septal defect and patent ductus arteriosus.
Objective To make epidemiological investigation on children and teenagers with congenital heart disease (CHD) from October 2015 to November 2016 in three counties in Zhaotong area. Methods The examination was divided into the following two phases: primary screening, secondary screening. Standardized echocardiographic examination was performed for the students with suspected CHD. we combined comprehensive analysis of electrocardiogram, X-ray and cardiac catheterization to make a clear diagnosis for patients. The index of prevalence rate of CHD, the types of the disease, and gender of the patients in 14957 children and teenagers were statistically analyzed. Results Altogether 61 cases of CHD were discovered. The total prevalence of CHD was 4.08‰ (61 of 14957 cases) , the prevalence rate in male was 4.55‰, in female was 3.61‰, No difference of the prevalence rate was found between male and female (χ2=0.823, P>0.05) ;the difference among different age groups had no statistical significance (χ2=4.692, P>0.05) ;The prevalence rate of CHD in Qiaojia was 5.17‰, in Daguan was 6.36‰, and in Zhenxiong was 3.03‰, There was no significant difference in the prevalence rate among different countries (χ2=5.897, P>0.05) . In terms of the types of the disease, atrial septal defect accounted for the first (37.7% ) , followed by ventricular septal defect (26.2% ) and patent ductus arteriosus (19.7% ) , others types of CHD were rare. Conclusion The prevalence of CHD in investigated area is slightly higher than the average level of the nationwide. The main types of CHD are atrial septal defect, ventricular septal defect and patent ductus arteriosus.
2018, 39(03): 81-84.
Abstract:
Objective To provide theoretical basis and data support for cleft lip and palate sequence therapy by analyzing the psychological status and characteristics of 7-15 years old children with Cleft Lip or Palate. Me thods 65 cases of 7-15 years old children with Cleft Lip or Palate who were in-patients of the 2 nd people's hospital of Yunnan province from Oct 2014 to Dec 2016 accepted two questionnaires survey: Self-esteem scale and social avoidance and distress scale. Re s ults (1) the proportion of high, moderate and low self-esteem was 27.7% , 49.2% and 23.1% , respectively, and the average score of male and female self-esteem was 29.818 2 and 27.285 7 respectively (P < 0.05) ; (2) The mean value of social avoidance and distress in children with cleft lip and palate of mean value was 11.492 3 and 11.492 3, respectively, which have significant differences with value "9"; the mean value between male and female of social avoidance were 10.772 7 and 13.000 0, and of social distress were 11.659 1 and 11.659 1, respectively, in both cases there is no significant difference between male and female (P>0.05) . Conclusion The distributions of self-esteem of children with cleft lip and palate were normal, and compared with the theoretical distribution, there was no significant difference. Male children had significantly higher self-esteem than female. Children with cleft lip and palate were more avoidant and anxious, and there was no significant difference between males and females.
Objective To provide theoretical basis and data support for cleft lip and palate sequence therapy by analyzing the psychological status and characteristics of 7-15 years old children with Cleft Lip or Palate. Me thods 65 cases of 7-15 years old children with Cleft Lip or Palate who were in-patients of the 2 nd people's hospital of Yunnan province from Oct 2014 to Dec 2016 accepted two questionnaires survey: Self-esteem scale and social avoidance and distress scale. Re s ults (1) the proportion of high, moderate and low self-esteem was 27.7% , 49.2% and 23.1% , respectively, and the average score of male and female self-esteem was 29.818 2 and 27.285 7 respectively (P < 0.05) ; (2) The mean value of social avoidance and distress in children with cleft lip and palate of mean value was 11.492 3 and 11.492 3, respectively, which have significant differences with value "9"; the mean value between male and female of social avoidance were 10.772 7 and 13.000 0, and of social distress were 11.659 1 and 11.659 1, respectively, in both cases there is no significant difference between male and female (P>0.05) . Conclusion The distributions of self-esteem of children with cleft lip and palate were normal, and compared with the theoretical distribution, there was no significant difference. Male children had significantly higher self-esteem than female. Children with cleft lip and palate were more avoidant and anxious, and there was no significant difference between males and females.
2018, 39(03): 85-89.
Abstract:
Objective To investigate the effectiveness of task-oriented bilateral arm training on upper limb function in children with spastic hemiplegia. Methods 27 children within 4-6 years with spastic hemiplegic were randomly divided into control group (n=13) and experiment group (n=14) . The children in control group received routine occupational therapy. The bilateral arm task-oriented training was added for children in experiment group. The Peabody development motor scale-2 (PDMS-2) and Upper Limb Physician's Rating Scale (ULPRS) were used to assess the upper limb function before and after 10 weeks intervention. Results After treatment, the scores of grasping function and visual-motor integration in PDMS-2 were significantly higher than those before treatment (P<0.05) , and the scores of experimental group werehigher than that of control group (P <0.05) , and the difference of the added value between two groups were statistically significant (P<0.05) .The ULPRS scores of both groups increased after treatment (P<0.05) , scores between the treatment group and the control group were not statistically significant (P>0.05) , and the difference of the added value between two groups was not statistically significant (P>0.05) . Conclusion The task-oriented bilateral arm training can promote recovery for upper limb and hand function in children with spastic hemiplegia compared with routine occupational therapy.
Objective To investigate the effectiveness of task-oriented bilateral arm training on upper limb function in children with spastic hemiplegia. Methods 27 children within 4-6 years with spastic hemiplegic were randomly divided into control group (n=13) and experiment group (n=14) . The children in control group received routine occupational therapy. The bilateral arm task-oriented training was added for children in experiment group. The Peabody development motor scale-2 (PDMS-2) and Upper Limb Physician's Rating Scale (ULPRS) were used to assess the upper limb function before and after 10 weeks intervention. Results After treatment, the scores of grasping function and visual-motor integration in PDMS-2 were significantly higher than those before treatment (P<0.05) , and the scores of experimental group werehigher than that of control group (P <0.05) , and the difference of the added value between two groups were statistically significant (P<0.05) .The ULPRS scores of both groups increased after treatment (P<0.05) , scores between the treatment group and the control group were not statistically significant (P>0.05) , and the difference of the added value between two groups was not statistically significant (P>0.05) . Conclusion The task-oriented bilateral arm training can promote recovery for upper limb and hand function in children with spastic hemiplegia compared with routine occupational therapy.
2018, 39(03): 90-94.
Abstract:
Objective To explore the efficacy of different surgeries for gastrointestinal stromal tumors (GIST) and their effects on perioperative indexesand prognosis. Methods 60 cases of GIST patients admitted in our hospital from October 2006 to November 2016 were selected and were divided into the study group (n =35, given the laparoscopic surgery) and the control group ( n =25, given the laparotomy) . The perioperative indexes, gastrointestinal function before and after operation [ (gastrin (GAS) , motilin (MTL) , vasoactive intestinal peptide (VIP) ], postoperative recurrence risk grade and complications were recorded in the two groups. Results There was no significant difference between the two groups in the operation time (P>0.05) , and the intraoperative blood loss volume and postoperative 3 d pain score in the study group were lower than those in the control group, and the postoperative exhaust time, eating time and hospital stay were shorter than those in the control group (P<0.05) . The levels of plasma GAS, MTL and VIP in the study group were higher than those in the control group after operation (P<0.05) . There was no significant difference in the postoperative recurrence risk grade and the incidence rate of complications between the two groups (P>0.05) . Conclusions Compared with the conventional laparotomy, laparoscopic surgery has better efficacy for GIST and has advantages of small trauma, mild pain and fast postoperative gastrointestinal function recovery, and it will not increase the risk of long-term recurrence and complications, therefore it has a good application prospect.
Objective To explore the efficacy of different surgeries for gastrointestinal stromal tumors (GIST) and their effects on perioperative indexesand prognosis. Methods 60 cases of GIST patients admitted in our hospital from October 2006 to November 2016 were selected and were divided into the study group (n =35, given the laparoscopic surgery) and the control group ( n =25, given the laparotomy) . The perioperative indexes, gastrointestinal function before and after operation [ (gastrin (GAS) , motilin (MTL) , vasoactive intestinal peptide (VIP) ], postoperative recurrence risk grade and complications were recorded in the two groups. Results There was no significant difference between the two groups in the operation time (P>0.05) , and the intraoperative blood loss volume and postoperative 3 d pain score in the study group were lower than those in the control group, and the postoperative exhaust time, eating time and hospital stay were shorter than those in the control group (P<0.05) . The levels of plasma GAS, MTL and VIP in the study group were higher than those in the control group after operation (P<0.05) . There was no significant difference in the postoperative recurrence risk grade and the incidence rate of complications between the two groups (P>0.05) . Conclusions Compared with the conventional laparotomy, laparoscopic surgery has better efficacy for GIST and has advantages of small trauma, mild pain and fast postoperative gastrointestinal function recovery, and it will not increase the risk of long-term recurrence and complications, therefore it has a good application prospect.
2018, 39(03): 95-97.
Abstract:
Objective To evaluate the effect of Vaseline lip smear in the treatment of patients with root canal treatment. Me thods We selected 60 patients who needed root canal treatment, and according to the randomized method, we randomly divided them into two groups: observation group and control group. The observation group used Vaseline lip smear, the control group was given Erythromycin Ointment coated lips. The clinical effect after root canal treatment were compared between two groups. Re s ults There was no cases of altercation ulcer or tear in the observation group after the root canal treatment, and 1 case of angular ulcer was found in the control group.Comparison between groups, the difference was not statistically significant, that of Vaseline and Erythromycin Ointment in preventing mouth ulcer or tear, no statistically significant differences between groups P>0.05. In the taste evaluation, there were 26 cases feeling good taste in the observation group, accounting for 86.67% , and 12 cases feeling good taste in the control group, accounting for 40% . There was statistically significant difference between two groups (P <0.05) . Conclus ion After the root canal treatment, the use of Vaseline can prevent chapped lips, ulcers, the effect is equivalent to Erythromycin Ointment, but in terms of taste, the patient more likely prefer Vaseline for lip moisturizer.
Objective To evaluate the effect of Vaseline lip smear in the treatment of patients with root canal treatment. Me thods We selected 60 patients who needed root canal treatment, and according to the randomized method, we randomly divided them into two groups: observation group and control group. The observation group used Vaseline lip smear, the control group was given Erythromycin Ointment coated lips. The clinical effect after root canal treatment were compared between two groups. Re s ults There was no cases of altercation ulcer or tear in the observation group after the root canal treatment, and 1 case of angular ulcer was found in the control group.Comparison between groups, the difference was not statistically significant, that of Vaseline and Erythromycin Ointment in preventing mouth ulcer or tear, no statistically significant differences between groups P>0.05. In the taste evaluation, there were 26 cases feeling good taste in the observation group, accounting for 86.67% , and 12 cases feeling good taste in the control group, accounting for 40% . There was statistically significant difference between two groups (P <0.05) . Conclus ion After the root canal treatment, the use of Vaseline can prevent chapped lips, ulcers, the effect is equivalent to Erythromycin Ointment, but in terms of taste, the patient more likely prefer Vaseline for lip moisturizer.
2018, 39(03): 98-102.
Abstract:
Objective To investigate the effect of glucosidorum tripterygll totorum combined with prednisone acetate and hydroxychloroquine sulfate in the treatment of systemic lupus erythematosus (SLE) . Me thods Eighty-two patients with SLE were divided into the control group (n=41) and the observation group (n=41) by the random number table method. The control group was treated with prednisone acetate combined with hydroxychloroquine sulfate. On the basis of treatment for the control group, the observation group was treated with glucosidorum tripterygll totorum. Four weeks was taken as 1 course of treatment and they were given 3 courses of treatment. Curative effect and adverse drug reactions were evaluated. The SLE disease activity index score was to evaluate the degree of lupus before and after treatment. The levels of blood lipid indexes [triglycerides (TG) , total cholesterol ( TC) , high density lipoprotein cholesterol ( HDL-C) , low density lipoprotein cholesterol (LDL-C) ], complement C3, immunoglobulin G (Ig G) , anti-double stranded DNA (ds-DNA) , interleukin 8 (IL-8) and interleukin 10 (IL-10) were determined. Re s ults The effective rate of the observation group was higher than that of the control group (92.50% vs 73.17% ) , P < 0.05. After treatment, the SLE-DAI score, levels of TC, TG, LDL-C, Ig G, anti-ds-DNA, IL-10 and IL-8 in the observation group were lower than those in the control group while levels of HDL-C and complement C3 were higher than those in the control group (all P < 0.05) . There was no significant difference in the incidence of adverse drug reactions between the two groups (56.10% vs 43.90% ) , P > 0.05. Conclusion The application of glucosidorum tripterygll totorum combined with prednisone acetate and hydroxychloroquine sulfate in the treatment of SLE can reduce the inflammatory and blood lipid levels and regulate immune mechanism. The effect is exact and patients can tolerate.
Objective To investigate the effect of glucosidorum tripterygll totorum combined with prednisone acetate and hydroxychloroquine sulfate in the treatment of systemic lupus erythematosus (SLE) . Me thods Eighty-two patients with SLE were divided into the control group (n=41) and the observation group (n=41) by the random number table method. The control group was treated with prednisone acetate combined with hydroxychloroquine sulfate. On the basis of treatment for the control group, the observation group was treated with glucosidorum tripterygll totorum. Four weeks was taken as 1 course of treatment and they were given 3 courses of treatment. Curative effect and adverse drug reactions were evaluated. The SLE disease activity index score was to evaluate the degree of lupus before and after treatment. The levels of blood lipid indexes [triglycerides (TG) , total cholesterol ( TC) , high density lipoprotein cholesterol ( HDL-C) , low density lipoprotein cholesterol (LDL-C) ], complement C3, immunoglobulin G (Ig G) , anti-double stranded DNA (ds-DNA) , interleukin 8 (IL-8) and interleukin 10 (IL-10) were determined. Re s ults The effective rate of the observation group was higher than that of the control group (92.50% vs 73.17% ) , P < 0.05. After treatment, the SLE-DAI score, levels of TC, TG, LDL-C, Ig G, anti-ds-DNA, IL-10 and IL-8 in the observation group were lower than those in the control group while levels of HDL-C and complement C3 were higher than those in the control group (all P < 0.05) . There was no significant difference in the incidence of adverse drug reactions between the two groups (56.10% vs 43.90% ) , P > 0.05. Conclusion The application of glucosidorum tripterygll totorum combined with prednisone acetate and hydroxychloroquine sulfate in the treatment of SLE can reduce the inflammatory and blood lipid levels and regulate immune mechanism. The effect is exact and patients can tolerate.
2018, 39(03): 103-108.
Abstract:
Objective To observe the application of enhanced recovery after surgery (ERAS) in complicated hepatic alveolar echinocoecosis surgery. Me thods We selected 117 patients from 2013 to 2016 and divided them into 3 groups in chronological order: Group A (n =35) , the traditional management group. All patients underwent perioperative management according to previous procedures. Group B (n=44) , the transitional management group. The management in the transition period was improved as far as possible in accordance with the ERAS programme. Group C (n=38) , the standard process group. All patients were treated in full accordance with standard process. The hemodynamics data, fluid volume and the perioperative complications were recorded in all groups. Re s ults Compared with group C, there was no significant difference in the general condition and the incidence of complications before operation, the anesthesia time, the operation time, blood loss, blood transfusion and the heart rate in group A and B. The incidence of hunger and thirst before operation, infusion volume, urine output, the incidence of postoperative complications and the average length of hospitalization were all higher than those in group C. The differences were statistically significant. Conclus ion The clinical path optimization of ERAS in liver hydatid surgery can significantly reduce the perioperative complications and reduce the average length of hospitalization.
Objective To observe the application of enhanced recovery after surgery (ERAS) in complicated hepatic alveolar echinocoecosis surgery. Me thods We selected 117 patients from 2013 to 2016 and divided them into 3 groups in chronological order: Group A (n =35) , the traditional management group. All patients underwent perioperative management according to previous procedures. Group B (n=44) , the transitional management group. The management in the transition period was improved as far as possible in accordance with the ERAS programme. Group C (n=38) , the standard process group. All patients were treated in full accordance with standard process. The hemodynamics data, fluid volume and the perioperative complications were recorded in all groups. Re s ults Compared with group C, there was no significant difference in the general condition and the incidence of complications before operation, the anesthesia time, the operation time, blood loss, blood transfusion and the heart rate in group A and B. The incidence of hunger and thirst before operation, infusion volume, urine output, the incidence of postoperative complications and the average length of hospitalization were all higher than those in group C. The differences were statistically significant. Conclus ion The clinical path optimization of ERAS in liver hydatid surgery can significantly reduce the perioperative complications and reduce the average length of hospitalization.
2018, 39(03): 109-114.
Abstract:
Objective To investigate the incidence and its main pathogenic bacteria infection status of food-borne diarrhea and analyze their influencing factors in Yunnan province from 2012 to 2016. Me thods 1743 cases of food-borne diarrhea were collected, which were supervised from 11 hospitals covering from 2012 to 2016 years in Yunnan province.We gathered and tested the biological samples. Meanwhile, we analyzed the main pathogenic bacteria and their influencing factors. Re s ults 65 positive strains samples were checked out in 1743 cases of food-borne diarrhea positive samples, the positive rate was 3.73% (65/1743) . Salmonella and Shigella strains were the main pathogenic bacteria, the main suspect food was meat and its products, as well as fruits and their products. In this study, Professions, methods of processing and clinical diagnosis were considered as the main factors of main pathogenic microbe detectable rates of food-borne diarrhea cases in Yunnan province. Conclus ion We should carry out public health education widespreadly in nursery, scattered children, farmers and migrant workers, students and other special crowds, provide intervention measures, attach great importance to food processing, reduce the possible contamination of food during processing, improve the level of clinical diagnosis and treatment and the recognization ability of food-borne diseases, and propose targeted prevention and control measures.Thus, food-borne disease can be prevented and controlled effectively.
Objective To investigate the incidence and its main pathogenic bacteria infection status of food-borne diarrhea and analyze their influencing factors in Yunnan province from 2012 to 2016. Me thods 1743 cases of food-borne diarrhea were collected, which were supervised from 11 hospitals covering from 2012 to 2016 years in Yunnan province.We gathered and tested the biological samples. Meanwhile, we analyzed the main pathogenic bacteria and their influencing factors. Re s ults 65 positive strains samples were checked out in 1743 cases of food-borne diarrhea positive samples, the positive rate was 3.73% (65/1743) . Salmonella and Shigella strains were the main pathogenic bacteria, the main suspect food was meat and its products, as well as fruits and their products. In this study, Professions, methods of processing and clinical diagnosis were considered as the main factors of main pathogenic microbe detectable rates of food-borne diarrhea cases in Yunnan province. Conclus ion We should carry out public health education widespreadly in nursery, scattered children, farmers and migrant workers, students and other special crowds, provide intervention measures, attach great importance to food processing, reduce the possible contamination of food during processing, improve the level of clinical diagnosis and treatment and the recognization ability of food-borne diseases, and propose targeted prevention and control measures.Thus, food-borne disease can be prevented and controlled effectively.
2018, 39(03): 115-118.
Abstract:
Objective To analysis the relationship between thyroid function with gastrointestinal motility and plasma motilin levels, and to provide the basis for clinical application.Me thods We selected 30 cases of patients with hyperthyroidism, 30 cases of hyperthyroidism group and hypothyroidism as hypothyroidism group, and 30 healthy volunteers as control group who were treated in our hospital from January 2013 to January 2016 in;The radioimmunoassay was used to test serum motilin levels, isotope single photon emission computed tomography was used to detected the gastric liquid technology half time, esophageal transit time.Re s ults The esophagus passage time of the subjects in the hypothyroidism group was significantly higher than that of the hyperthyroidism group and the control group, and the difference was statistically significant (P<0.05) ;The level of motilin in serum of hyperthyroidism group was significantly higher than that of control group (P<0.05) , and the level of motilin in serum of hypothyroidism group was significantly lower than that of control group (P<0.05) ;Hyperthyroidism was positively correlated with motilin level (r = 0.542, P<0.05) , and negatively correlated with the time of passage of the esophagus and gastric emptying time (r =-0.126 and r =-0.481, P<0.05) ;Hypothyroidism was negatively correlated with motilin level (r =-0.617, P<0.05) , and positively correlated with esophageal transit time and gastric emptying time (r = 0.627 and r = 0.416, P <0.05) .Conclus ions Hyperthyroidism can lead to the increase of gastrointestinal function and motilin level. Hypothyroidism can lead to the decrease of gastrointestinal function and motilin level.
Objective To analysis the relationship between thyroid function with gastrointestinal motility and plasma motilin levels, and to provide the basis for clinical application.Me thods We selected 30 cases of patients with hyperthyroidism, 30 cases of hyperthyroidism group and hypothyroidism as hypothyroidism group, and 30 healthy volunteers as control group who were treated in our hospital from January 2013 to January 2016 in;The radioimmunoassay was used to test serum motilin levels, isotope single photon emission computed tomography was used to detected the gastric liquid technology half time, esophageal transit time.Re s ults The esophagus passage time of the subjects in the hypothyroidism group was significantly higher than that of the hyperthyroidism group and the control group, and the difference was statistically significant (P<0.05) ;The level of motilin in serum of hyperthyroidism group was significantly higher than that of control group (P<0.05) , and the level of motilin in serum of hypothyroidism group was significantly lower than that of control group (P<0.05) ;Hyperthyroidism was positively correlated with motilin level (r = 0.542, P<0.05) , and negatively correlated with the time of passage of the esophagus and gastric emptying time (r =-0.126 and r =-0.481, P<0.05) ;Hypothyroidism was negatively correlated with motilin level (r =-0.617, P<0.05) , and positively correlated with esophageal transit time and gastric emptying time (r = 0.627 and r = 0.416, P <0.05) .Conclus ions Hyperthyroidism can lead to the increase of gastrointestinal function and motilin level. Hypothyroidism can lead to the decrease of gastrointestinal function and motilin level.
2018, 39(03): 119-124.
Abstract:
Growth arrest-specific 6, which is a member of the vitamin K-dependent protein family, is a ligand of TAM (Tyro3, Axl, Mer) belonging to the receptor tyrosine kinase family.Gas6/Axl signaling pathways are involved in pathophysiological processes of cell adhesion, migration, proliferation, apoptosis, platelet aggregation, vascular remodeling, inflammatory and immune responses. In recent years, studies found that Gas6/Axl signaling pathway, Gas6 level and the gene polymorphism of Gas6 were significantly associated with type 2 diabetes, obesity, insulin resistance and diabetic complications.This article reviews the correlation between Gas6 and type 2 diabetes and its complications
Growth arrest-specific 6, which is a member of the vitamin K-dependent protein family, is a ligand of TAM (Tyro3, Axl, Mer) belonging to the receptor tyrosine kinase family.Gas6/Axl signaling pathways are involved in pathophysiological processes of cell adhesion, migration, proliferation, apoptosis, platelet aggregation, vascular remodeling, inflammatory and immune responses. In recent years, studies found that Gas6/Axl signaling pathway, Gas6 level and the gene polymorphism of Gas6 were significantly associated with type 2 diabetes, obesity, insulin resistance and diabetic complications.This article reviews the correlation between Gas6 and type 2 diabetes and its complications
2018, 39(03): 125-131.
Abstract:
Ileal ureter is a suitable treatment option for patients with long ureteric strictures. Minimally invasive techniques have been shown to be as safe as open techniques but superior in terms of lesser trauma, less bleeding and less postoperative recovery time. In order to evaluate the feasibility and clinical efficacy of laparoscopic ileal ureter replacement, review of related literatures, indications, contraindications, surgical method, postoperative results combined with experience of our center has been done. Total 20 cases of laparoscopic ileal ureteral operation have been reviewed, the mean operation time was 195480 and blood loss was 50 ~ 375 ml and 7 Robotic ileal ureter replacement were reviewed median surgery time was 320720 min and bleeding is less then 10 ml100 ml. The operation complications include urinary leakage, intestinal obstruction, anastomotic site leakage, transient azotemia, urinary tract infection and so on. Postoperative renal function of all patients was improved in different degrees. Laparoscopic and Robotic ileal ureter replacement is feasible and can be used as an effective treatment for long ureteral injuries, although the curative effect is same as that of traditional open surgery, but Comparatively laparoscopic and robotic postoperative morbidity is less and have advantages such as it reduces surgical trauma, intraoperative bleeding, postoperative complications, less surgical scar and recovery time.The cases in the reports are limited, more cases need to be studied, and long-term results need to be assessed to establish and standardize these technique.
Ileal ureter is a suitable treatment option for patients with long ureteric strictures. Minimally invasive techniques have been shown to be as safe as open techniques but superior in terms of lesser trauma, less bleeding and less postoperative recovery time. In order to evaluate the feasibility and clinical efficacy of laparoscopic ileal ureter replacement, review of related literatures, indications, contraindications, surgical method, postoperative results combined with experience of our center has been done. Total 20 cases of laparoscopic ileal ureteral operation have been reviewed, the mean operation time was 195480 and blood loss was 50 ~ 375 ml and 7 Robotic ileal ureter replacement were reviewed median surgery time was 320720 min and bleeding is less then 10 ml100 ml. The operation complications include urinary leakage, intestinal obstruction, anastomotic site leakage, transient azotemia, urinary tract infection and so on. Postoperative renal function of all patients was improved in different degrees. Laparoscopic and Robotic ileal ureter replacement is feasible and can be used as an effective treatment for long ureteral injuries, although the curative effect is same as that of traditional open surgery, but Comparatively laparoscopic and robotic postoperative morbidity is less and have advantages such as it reduces surgical trauma, intraoperative bleeding, postoperative complications, less surgical scar and recovery time.The cases in the reports are limited, more cases need to be studied, and long-term results need to be assessed to establish and standardize these technique.
2018, 39(03): 132-136.
Abstract:
Objective To analyze the problems and reasons in the clinical medicine postgraduate cultivation.We stated the competency of clinical medicine postgraduates according to the transition of health demands global medical development and practical requirements for clinical personnel. Me thods Questionnaire surveys combined with interviews were conducted, and statistical analysis was performed. In the first part, we selected the permanent staff with qualifications of medical clinicians, including: chief physicians, associate chief physicians, physicians, residents, accounting for 72% of the interviewees; And related personnel, including nurses, administrators and patients, were also interviewed, accounting for 28% of the total. 90 questionnaires were distributed, and 86 valid questionnaires were recovered, with an effective rate of 95.56% . In the second part, graduate students with clinical professional degrees were selected for interviews, 96 questionnaires were distributed, and 92 valid questionnaires were collected, and the effective rate was 95.83% .Re s ults There was a certain gap between the expected goal and the actual situation of the post competency of clinical professional degree graduate students, and the gap had a certain statistical significance. The clinical professional degree graduate students paid more attention to the doctor-patient communication, teamwork. Conclus ions We elaborated the required post competence for clinical medicine postgraduates on the basis of its content and characteristic. We propose to strengthen the cultivation of clinical medicine postgraduates by the reform and support of the government enhanced construction of teachers improved course plan and cultivation process perfected assessment system in order to make the students competent to their posts.
Objective To analyze the problems and reasons in the clinical medicine postgraduate cultivation.We stated the competency of clinical medicine postgraduates according to the transition of health demands global medical development and practical requirements for clinical personnel. Me thods Questionnaire surveys combined with interviews were conducted, and statistical analysis was performed. In the first part, we selected the permanent staff with qualifications of medical clinicians, including: chief physicians, associate chief physicians, physicians, residents, accounting for 72% of the interviewees; And related personnel, including nurses, administrators and patients, were also interviewed, accounting for 28% of the total. 90 questionnaires were distributed, and 86 valid questionnaires were recovered, with an effective rate of 95.56% . In the second part, graduate students with clinical professional degrees were selected for interviews, 96 questionnaires were distributed, and 92 valid questionnaires were collected, and the effective rate was 95.83% .Re s ults There was a certain gap between the expected goal and the actual situation of the post competency of clinical professional degree graduate students, and the gap had a certain statistical significance. The clinical professional degree graduate students paid more attention to the doctor-patient communication, teamwork. Conclus ions We elaborated the required post competence for clinical medicine postgraduates on the basis of its content and characteristic. We propose to strengthen the cultivation of clinical medicine postgraduates by the reform and support of the government enhanced construction of teachers improved course plan and cultivation process perfected assessment system in order to make the students competent to their posts.
2018, 39(03): 137-140.
Abstract:
Objective To study the effect of continuous nursing on the growth and development of extremely low birth weight preterm infants. Me thods 80 cases of extremely low birth weight preterm infants treated in the Dept. of Pediatrics, The Third People's Hospital of Yunnan Province from October 2015 to October 2016, were selected and divided into 2 groups: experimental group and control group, 40 cases in each group. The control group was given conventional nursing, the experimental group was given continuous nursing. Then the growth and development of infants in the two groups were compared. Re s ults The weight, body length, head circumference in the experimental group were significantly better than the control group, the difference was statistically significant (P < 0.05) . The readmission rate in 6 months of infants in the experimental group was lower and the parents satisfaction was significantly higher than that of control group, the difference was statistically significant (P <0.05) . Conclus ion Conventional nursing has significant effect for extremely low birth weight preterm infants, it can improve the growth and development of infants, reduce the hospital readmission rate, and improve parents' satisfaction.
Objective To study the effect of continuous nursing on the growth and development of extremely low birth weight preterm infants. Me thods 80 cases of extremely low birth weight preterm infants treated in the Dept. of Pediatrics, The Third People's Hospital of Yunnan Province from October 2015 to October 2016, were selected and divided into 2 groups: experimental group and control group, 40 cases in each group. The control group was given conventional nursing, the experimental group was given continuous nursing. Then the growth and development of infants in the two groups were compared. Re s ults The weight, body length, head circumference in the experimental group were significantly better than the control group, the difference was statistically significant (P < 0.05) . The readmission rate in 6 months of infants in the experimental group was lower and the parents satisfaction was significantly higher than that of control group, the difference was statistically significant (P <0.05) . Conclus ion Conventional nursing has significant effect for extremely low birth weight preterm infants, it can improve the growth and development of infants, reduce the hospital readmission rate, and improve parents' satisfaction.
2018, 39(03): 141-144.
Abstract:
Objecctive To explore the construction and effect of the Doctor-nurse integrative medical care mode for domestic analgesia in middle and late stage cancer patients.Me thods From september 2016 to February 2017, 120 Cancer patients in The People's hospitalof Du Jiang Yan were included, and randomly divided into experimental group (n=60) and observation group (n=60) . The observation group received routine outpatient follow-up after discharge.The experimental group was treated with the Doctor-nurse integrative medical care mode.The analgesic modes included psychological support, immediate morphine and morphine sustained release tablets for personalized home titration. The Net bottom-hospitals were responsible for the follow-up and intervention, the training and guidance were bore by Hub hospitals. The patients were followed up at the first and twelfth weeks, followed by telephone follow-up at fourth and eighthweek, after discharge. The quality of life, the degree of depression and the degree of anxiety of caregiver were compared between the two groups at the beginning of the study and the twelfth weeks after discharge. Re s ults In experimental group, the scores of in the life quality of patints before and after intervention were (62.43±12.83) and (50.33 ±9.04) , respectively, the scores of depression before and after intervention were (50.33± 6.59) and (47.37±4.97) , respectively, the scores of anxiety before and after intervention were (55.05 ±8.82) and (52.22 ±5.37) . There was statistically significant difference between the two groups (P<0.05) . Conclus ions Doctor-nurse integrative medical care mode can improve the quality of life of patients with advanced cancer, reduce the degree of depression, and reduce the degree of anxiety of patients.
Objecctive To explore the construction and effect of the Doctor-nurse integrative medical care mode for domestic analgesia in middle and late stage cancer patients.Me thods From september 2016 to February 2017, 120 Cancer patients in The People's hospitalof Du Jiang Yan were included, and randomly divided into experimental group (n=60) and observation group (n=60) . The observation group received routine outpatient follow-up after discharge.The experimental group was treated with the Doctor-nurse integrative medical care mode.The analgesic modes included psychological support, immediate morphine and morphine sustained release tablets for personalized home titration. The Net bottom-hospitals were responsible for the follow-up and intervention, the training and guidance were bore by Hub hospitals. The patients were followed up at the first and twelfth weeks, followed by telephone follow-up at fourth and eighthweek, after discharge. The quality of life, the degree of depression and the degree of anxiety of caregiver were compared between the two groups at the beginning of the study and the twelfth weeks after discharge. Re s ults In experimental group, the scores of in the life quality of patints before and after intervention were (62.43±12.83) and (50.33 ±9.04) , respectively, the scores of depression before and after intervention were (50.33± 6.59) and (47.37±4.97) , respectively, the scores of anxiety before and after intervention were (55.05 ±8.82) and (52.22 ±5.37) . There was statistically significant difference between the two groups (P<0.05) . Conclus ions Doctor-nurse integrative medical care mode can improve the quality of life of patients with advanced cancer, reduce the degree of depression, and reduce the degree of anxiety of patients.
2018, 39(03): 145-148.
Abstract:
Objective To explore the effect of stratified chain nursing model on the efficacy, symptom improvement and nursing satisfaction in pediatric pneumonia nursing. Me thods 58 cases of pediatric pneumonia in our hospital from March 2015 to March 2016 were selected as the control group. 58 cases of pediatric pneumonia in our hospital from April to April 2017 were selected as observation group. After 2 weeks of care, the two groups were compared in the curative effect, the symptoms improved and the nursing satisfaction. Re s ults The total effective rate of the two groups was significantly higher than that of the control group (P<0.05) . The disappearance time of fever, the time of disappearance of cough, the time of disappearance of lung rales and the time of hospitalization were shorter than those of the control group, the difference was statistically significant (P <0.05) . The observation group had higher evaluation scores in ward environment, nursing technology, sense of responsibility, health education, psychological care, and service attitude than the control group, the difference was statistically significant (P <0.05) . Conclus ion Hierarchical chain nursing model is effective in the treatment of pediatric pneumonia, which can effectively improve the curative effect and nursing satisfaction, which is beneficial to the improvement of symptoms.
Objective To explore the effect of stratified chain nursing model on the efficacy, symptom improvement and nursing satisfaction in pediatric pneumonia nursing. Me thods 58 cases of pediatric pneumonia in our hospital from March 2015 to March 2016 were selected as the control group. 58 cases of pediatric pneumonia in our hospital from April to April 2017 were selected as observation group. After 2 weeks of care, the two groups were compared in the curative effect, the symptoms improved and the nursing satisfaction. Re s ults The total effective rate of the two groups was significantly higher than that of the control group (P<0.05) . The disappearance time of fever, the time of disappearance of cough, the time of disappearance of lung rales and the time of hospitalization were shorter than those of the control group, the difference was statistically significant (P <0.05) . The observation group had higher evaluation scores in ward environment, nursing technology, sense of responsibility, health education, psychological care, and service attitude than the control group, the difference was statistically significant (P <0.05) . Conclus ion Hierarchical chain nursing model is effective in the treatment of pediatric pneumonia, which can effectively improve the curative effect and nursing satisfaction, which is beneficial to the improvement of symptoms.
2018, 39(03): 49-54.
Abstract:
Objective To understand the status quo of health resource allocation of medical institutions in poverty-stricken areas, and to provide decision-making basis for rationalizing health and poverty alleviation policies and improving the overall service capacity of medical institutions in poverty-stricken areas. Me thods The overall institutions, bed capacity and staffs in medical institutions in 680 poor counties were analyzed. Re s ults The proportion of government health expenditure in 14 concentrated areas was lower than 15% . The largest number of health institutions was 349 in the Dabie Mountains and 70 in Xizang, and the number of beds was lower than the national average level of 5.11.The largest number of health technical staff for 1 000 people of the four provinces is 4.42 people, the smallest number is 2.72 in Wumeng mountain area;the registered nurses (number) for 1 000 people is up to 1.56 people in the Luo Xia mountain area, the lowest Xizang, only 0.39 people. Thousands of population practice (assistant) physician number of Tibetans is up to 2.98 people, the lowest is 1.07 for the Xinjiang Southern Xinjiang three states; health care than the lowest in Xizang 1:0.54. Conclus ion At present, China's centralized contiguous poverty-stricken areas of county-level medical institutions is extremely short of resources, and the health resource allocation is uneven.
2018, 39(03): 62-66.
Abstract:
Objective To investigate the relationship between proportion of Tumor infiltrating lymphocytes (TILs) in the prostate tumor mass and postoperative recurrence of prostate carcinoma in order to offer the theoretic as well as experimental basis for the prevention of postoperative recurrence in the prostate carcinoma.Methods The proportion of CD3+, CD8+, CD3+CD4+, CD3+CD8+ TILs cells of 81 PCa specimens underwent Radical prostatectomy were analyzed. The proportion of CD3+CD8+TILs in patients with recurrence of PCa within3 years after surgery (group A), within 35 years (group B) and without recurrence after 5 years (group C) was compared. Results The proportion of CD3+CD8+TILs was significantly lower in group A than that of in group B and C (P<0.05), meanwhile, the proportion of CD3+CD8+TILs were significantly higher in group C than that in group A and B (P<0.05), while="" the="" proportion="" of="" in="" three="" groups="" had="" no="" significant="" difference="" p="">0.05). Conclusion The proportion of CD3+CD8+TILs presented in the PCa specimen mass is relative to the postoperative recurrence of prostate cancer, which might be a biomarker for the surveillance of postoperative recurrence of PCa.
