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RSS2018 Vol. 39, No. 04
2018, 39(04): 1-4.
Abstract:
Objective To construct GST-SRY fusion protein expression vector and induce its expression in E.coli, then purificate so as to obtain GST-SRY fusion protein. Me thods SRY gene was amplified by PCR and cloned into prokaryotic expression vector p GEX-6 P-1. p GEX-6 P-1-SRY plasmid was verified by restriction enzyme digestion and DNA sequencing. After that, the recombinant plasmid was transformed into E.coli Rosetta DE3.Expression of GST-SRY fusion protein was induced by IPTG, which was purified by Glutathione Sepharose later on.Re s ults Restriction enzyme digestion and sequencing demonstrated that p GEX-6 P-1-SRY was successfully obtained. The result of SDS-PAGE assay indicated that SRY was successfully expressed in E.coli Rosetta DE3 and expressed a 49 KD SRY protein.Conclus ion The successful construction of SRY gene recombinant prokaryotic expression vector and expression of SRY protein will be helpful for further studies of SRY gene function.
Objective To construct GST-SRY fusion protein expression vector and induce its expression in E.coli, then purificate so as to obtain GST-SRY fusion protein. Me thods SRY gene was amplified by PCR and cloned into prokaryotic expression vector p GEX-6 P-1. p GEX-6 P-1-SRY plasmid was verified by restriction enzyme digestion and DNA sequencing. After that, the recombinant plasmid was transformed into E.coli Rosetta DE3.Expression of GST-SRY fusion protein was induced by IPTG, which was purified by Glutathione Sepharose later on.Re s ults Restriction enzyme digestion and sequencing demonstrated that p GEX-6 P-1-SRY was successfully obtained. The result of SDS-PAGE assay indicated that SRY was successfully expressed in E.coli Rosetta DE3 and expressed a 49 KD SRY protein.Conclus ion The successful construction of SRY gene recombinant prokaryotic expression vector and expression of SRY protein will be helpful for further studies of SRY gene function.
2018, 39(04): 5-10.
Abstract:
Objective To explore the effect of Edaravone (EDA) on oxidative stress such as release of nitric oxide (NO) induced by Lipopolysaccharide (LPS) in activation of primary microglia. Me thods There were four groups including control, LPS, LPS + EDA and MAPK inhibitors + EDA + LPS in this study. The primary mixed glial cells were derived from the cortex of Sprague-Dawley rats which were 2 to 3-day old neonatal pups. The primary microglia were separated and purified by Biological Shaker after growing for about ten days. Griess Assay was used to detect NO secreted by activated microglia in four groups. Re s ults The shake-off microglial cells were confirmed as purity of 95% or up via fluorescence staining of CD68. Compared with the control group, NO product of microglial activation mediated by LPS significantly increased while NO value decreased in the group of EDA which was pretreatedt of EDA ahead of LPS for 24 hrs (P<0.05) . After addition of LPS, the incubation was remained for additional 24 h. In addition to EDA pretreatment for 24 h, the addition of three Mitogen-activated protein kinase inhibitors of MAPK signaling pathways were ahead of addition of EDA for 30 min, and then 10 ng/m Lof LPS was added to microglia. All of them incubated with the primary microglia for 24 h after the last addition. NO release was significantly reduced, the difference was statistically significant (P <0.05) .Conclus ion EDA treatment alone could inhibit the release of NO induced by LPS in primary microglia while EDA plus MAPK signaling pathway inhibitor obviously reduced release of NO. EDA may take effect with p38 MAPK, JNK and ERK inhibitors of sub-pathways in some way (s) to co-operate with EDA in modulating oxidative stress in activated microglia induced by LPS. As to the working mechanisms how EDA co-operates with MAPK inhibitors is still remain unknown.
Objective To explore the effect of Edaravone (EDA) on oxidative stress such as release of nitric oxide (NO) induced by Lipopolysaccharide (LPS) in activation of primary microglia. Me thods There were four groups including control, LPS, LPS + EDA and MAPK inhibitors + EDA + LPS in this study. The primary mixed glial cells were derived from the cortex of Sprague-Dawley rats which were 2 to 3-day old neonatal pups. The primary microglia were separated and purified by Biological Shaker after growing for about ten days. Griess Assay was used to detect NO secreted by activated microglia in four groups. Re s ults The shake-off microglial cells were confirmed as purity of 95% or up via fluorescence staining of CD68. Compared with the control group, NO product of microglial activation mediated by LPS significantly increased while NO value decreased in the group of EDA which was pretreatedt of EDA ahead of LPS for 24 hrs (P<0.05) . After addition of LPS, the incubation was remained for additional 24 h. In addition to EDA pretreatment for 24 h, the addition of three Mitogen-activated protein kinase inhibitors of MAPK signaling pathways were ahead of addition of EDA for 30 min, and then 10 ng/m Lof LPS was added to microglia. All of them incubated with the primary microglia for 24 h after the last addition. NO release was significantly reduced, the difference was statistically significant (P <0.05) .Conclus ion EDA treatment alone could inhibit the release of NO induced by LPS in primary microglia while EDA plus MAPK signaling pathway inhibitor obviously reduced release of NO. EDA may take effect with p38 MAPK, JNK and ERK inhibitors of sub-pathways in some way (s) to co-operate with EDA in modulating oxidative stress in activated microglia induced by LPS. As to the working mechanisms how EDA co-operates with MAPK inhibitors is still remain unknown.
2018, 39(04): 11-15.
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Objective To investigate the regulation of scutellarin (SCU) on protein and m RNA expression of PKCε in Human cardiac microvascular endothelial cells (HCMECs) induced by hypoxia reoxygenation or normal. Me thods We cultured HCMECs in two ways: normal and hypoxia reoxygenation (HR) . The normal groups were divided into one control group and three SCU (0.1 μM, 1 μM, 10 μM) groups;HR treatment induded one control group and four groups of HR, HR+ SCU 0.1 μM, HR+SCU 1 μM and HR+SCU 10 μM. Protein and m RNA expression of PKCε was determined by Western blot and RT-PCR respectively.Re s ults In normal cultured, SCU (0.1 μM, 1 μM and 10 μM) significantly up-regulated the protein and m RNA expression of PKCε (P <0.05) , and for p-PKCε, there was an up-regulated tendency. In the HR treatment, the model group had a down regulation tendency, but SCU 10 μM group could significantly up-regulate the protein and m RNA expression of PKCε (P <0.05) , and SCU 10 μM also could significantly up-regulated p-PKCε (P <0.05) . Conclus ion SCU up-regulates the expression of PKCε protein and m RNA in HCMECs induced by hypoxia reoxygenation or normal.
Objective To investigate the regulation of scutellarin (SCU) on protein and m RNA expression of PKCε in Human cardiac microvascular endothelial cells (HCMECs) induced by hypoxia reoxygenation or normal. Me thods We cultured HCMECs in two ways: normal and hypoxia reoxygenation (HR) . The normal groups were divided into one control group and three SCU (0.1 μM, 1 μM, 10 μM) groups;HR treatment induded one control group and four groups of HR, HR+ SCU 0.1 μM, HR+SCU 1 μM and HR+SCU 10 μM. Protein and m RNA expression of PKCε was determined by Western blot and RT-PCR respectively.Re s ults In normal cultured, SCU (0.1 μM, 1 μM and 10 μM) significantly up-regulated the protein and m RNA expression of PKCε (P <0.05) , and for p-PKCε, there was an up-regulated tendency. In the HR treatment, the model group had a down regulation tendency, but SCU 10 μM group could significantly up-regulate the protein and m RNA expression of PKCε (P <0.05) , and SCU 10 μM also could significantly up-regulated p-PKCε (P <0.05) . Conclus ion SCU up-regulates the expression of PKCε protein and m RNA in HCMECs induced by hypoxia reoxygenation or normal.
2018, 39(04): 16-20.
Abstract:
Object To detect the effect of terlipressin and FK-409 on protection of liver graft injury after reduced-size liver transplantation in rats. Me thods We performed liver transplantation using 30% volume liver graft in rats. ALT, total bilirubin, portal vein pressure and survival rate were detected after operation. We divided recipient rats into four groups including terlipressin treatment group, FK-409 treatment group, terlipressin groups and FK-409 treatment group. ALT, total bilirubin, portal vein pressure and survival rate were detected in each group after operation. We compared results of four groups and found potential therapy which protected liver graft injury after reduced-size liver transplantation. Re s ults In reduced-size liver transplantation model, terlipressin and FK-409 treatment group showed lower portal vein pressure (P <0.05) , lower level of ALT and bilirubin (P<0.05) and longer survival time (P<0.05) compared with the control group. Using terlipressin and FK-409 could reduce liver graft injury after reduced-size liver transplantation. Conclus ion Using terlipressin and FK-409 could reduced liver graft injury, which might be the potential therapy for small-for-size syndrome after reduced-size liver transplantation.
Object To detect the effect of terlipressin and FK-409 on protection of liver graft injury after reduced-size liver transplantation in rats. Me thods We performed liver transplantation using 30% volume liver graft in rats. ALT, total bilirubin, portal vein pressure and survival rate were detected after operation. We divided recipient rats into four groups including terlipressin treatment group, FK-409 treatment group, terlipressin groups and FK-409 treatment group. ALT, total bilirubin, portal vein pressure and survival rate were detected in each group after operation. We compared results of four groups and found potential therapy which protected liver graft injury after reduced-size liver transplantation. Re s ults In reduced-size liver transplantation model, terlipressin and FK-409 treatment group showed lower portal vein pressure (P <0.05) , lower level of ALT and bilirubin (P<0.05) and longer survival time (P<0.05) compared with the control group. Using terlipressin and FK-409 could reduce liver graft injury after reduced-size liver transplantation. Conclus ion Using terlipressin and FK-409 could reduced liver graft injury, which might be the potential therapy for small-for-size syndrome after reduced-size liver transplantation.
2018, 39(04): 21-26.
Abstract:
Objective To explore the influence of CD151 inhibition by RNA interference on lung metastasis in null mice given tail veins injection of lung adenocarcinoma cell lines A549. METHODS: Constructed CD151 inhibition by RNA interference cell lines A549-sh RNA, A549-sh NC and A549-Blank were injected into the tail veins of BALB/c nude mice. Lung metastasis was measured through naked eye and pathology of HE stain. Re s ults Lung metastasis were 62.5% (5/8) and (4.7±1.5) , 100% (7/7) and (9.3±0.8) , 75% (6/8) and (8.2±1.5) , respectively in A549-sh RNA, A549-sh NC and A549-Blank. There were differences between A549-sh RNA and A549-sh NC (P = 0.000) and between A549-sh RNA and A549-Blank (P = 0.006) .Conclus ions Inhibition of CD151 gene expression can reduce lung metastasis in null mice given tail veins injection of lung adenocarcinoma cell lines A549. CD151 may become one of the potential therapeutic targets aiming at inhibitung invasion and metastasis of NSCLC.
Objective To explore the influence of CD151 inhibition by RNA interference on lung metastasis in null mice given tail veins injection of lung adenocarcinoma cell lines A549. METHODS: Constructed CD151 inhibition by RNA interference cell lines A549-sh RNA, A549-sh NC and A549-Blank were injected into the tail veins of BALB/c nude mice. Lung metastasis was measured through naked eye and pathology of HE stain. Re s ults Lung metastasis were 62.5% (5/8) and (4.7±1.5) , 100% (7/7) and (9.3±0.8) , 75% (6/8) and (8.2±1.5) , respectively in A549-sh RNA, A549-sh NC and A549-Blank. There were differences between A549-sh RNA and A549-sh NC (P = 0.000) and between A549-sh RNA and A549-Blank (P = 0.006) .Conclus ions Inhibition of CD151 gene expression can reduce lung metastasis in null mice given tail veins injection of lung adenocarcinoma cell lines A549. CD151 may become one of the potential therapeutic targets aiming at inhibitung invasion and metastasis of NSCLC.
2018, 39(04): 27-33.
Abstract:
Objective To establish animal model of non-alcoholic fatty liver disease (NAFLD) and explore the molecular mechanism of homocysteine changes in SD rats with NAFLD by ginsenoside Rg1 treating. Me thods NAFLD animal model was established to determine serum triglyceride and total cholesterol in the fasting state. HE staining was used to detect the morphological changes of the liver, and lipidosis of the liver tissue was observed by the oil red "o" staining in the control group and model control group. The serous concentration of homocysteine wasmeasured in every group by ELISA. Immunohistochemistry and Western blot were used to detect the expression levels of methylenetetrahydrofolate reductase (MTHFR) and cystathionine synthetase (CBS) in liver tissues. Results The concentrations of triglyceride and total cholesterol in the model control group were disordered compared with the control group (P<0.05) , meanwhile the morphology of the liver tissue was disordered and lipidosis was obvious in the model control group. So those results indicated the NAFLD model was successful. The concentration of homocysteine in the model control group was increased and decreased by Ginsenoside Rg1 treating (P<0.05) . The protein expressions of MTHER and CBS in model control group were lower compared with the control group by immunohistochemistry and Western blot (P<0.05) . The protein expressions ofMTHFR and CBS in the ginsenoside Rg1 treatment group were higher compared with the model control group by immunohistochemistry and Western blot (P<0.05) . The results have dose-dependent. Conclusion Ginsenoside Rg1 is possible to increase the protein expressions of MTHFR and CBS and decrease the concentration of homocysteine in animal models of non-alcoholic fatty liver, thus it can play a protective effect in non-alcoholic fatty liver.
Objective To establish animal model of non-alcoholic fatty liver disease (NAFLD) and explore the molecular mechanism of homocysteine changes in SD rats with NAFLD by ginsenoside Rg1 treating. Me thods NAFLD animal model was established to determine serum triglyceride and total cholesterol in the fasting state. HE staining was used to detect the morphological changes of the liver, and lipidosis of the liver tissue was observed by the oil red "o" staining in the control group and model control group. The serous concentration of homocysteine wasmeasured in every group by ELISA. Immunohistochemistry and Western blot were used to detect the expression levels of methylenetetrahydrofolate reductase (MTHFR) and cystathionine synthetase (CBS) in liver tissues. Results The concentrations of triglyceride and total cholesterol in the model control group were disordered compared with the control group (P<0.05) , meanwhile the morphology of the liver tissue was disordered and lipidosis was obvious in the model control group. So those results indicated the NAFLD model was successful. The concentration of homocysteine in the model control group was increased and decreased by Ginsenoside Rg1 treating (P<0.05) . The protein expressions of MTHER and CBS in model control group were lower compared with the control group by immunohistochemistry and Western blot (P<0.05) . The protein expressions ofMTHFR and CBS in the ginsenoside Rg1 treatment group were higher compared with the model control group by immunohistochemistry and Western blot (P<0.05) . The results have dose-dependent. Conclusion Ginsenoside Rg1 is possible to increase the protein expressions of MTHFR and CBS and decrease the concentration of homocysteine in animal models of non-alcoholic fatty liver, thus it can play a protective effect in non-alcoholic fatty liver.
2018, 39(04): 34-39.
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Objective To establish an oxidative stress cell model by inducing human umbilical vein endothelial cells (HUVECs) using hydrogen peroxide (H2 O2) . Methods Six gradient concentrations of H2 O2 were co-cultured with HUVECs for 6 h, 12 h, 24 h and 48 h. Inverted microscope was used to observe the change of cell morphologies. The optimal working concentration and effect time of H2 O2 on HUVECs were screened by CCK-8 method for cell viability, flowcymetry for apoptosis rate, and CDFU-DA fluorescent probing and flowcymetry for ROS levels. Re s ults Any concentration of H2 O2 could do harm to HUVECs co-culturing with cells. Cell viabilities decreased statistically when treating cells with 400, 600 or 800 μmol/L H2 O2 for different times (P<0.05) . Cell viabilities also decreased gradually as time prolonged when treating cells with 800 μmol/L H2 O2 (P<0.001) . When treating cells with different concentration of H2 O2 for 24 h, cell apoptosis rates increased dramatically (1000μmol/L as an exception, P<0.05) , so did cell necrosis rates (100 μmol/L as an exception, P<0.05) ;When treating cells with 800 μmol/L H2 O2, cell apoptosis rates increased dramatically as time prolonged, reaching the peak at 24 h (P<0.05) , so did necrosis rates, reaching the peak at 48 h (P<0.05) . ROS level reached thepeak while treating cells with 400 μmol/L H2 O2 (P<0.001) ; ROS levels increased gradully as time prolonged when treating cells with 800 μmol/L H2 O2 (P<0.001) . Conclusions The oxidative stress model in HUVECs was established by coculturing cells with culture medium containing 800 μmol/L hydrogen peroxide for 24 h.
Objective To establish an oxidative stress cell model by inducing human umbilical vein endothelial cells (HUVECs) using hydrogen peroxide (H2 O2) . Methods Six gradient concentrations of H2 O2 were co-cultured with HUVECs for 6 h, 12 h, 24 h and 48 h. Inverted microscope was used to observe the change of cell morphologies. The optimal working concentration and effect time of H2 O2 on HUVECs were screened by CCK-8 method for cell viability, flowcymetry for apoptosis rate, and CDFU-DA fluorescent probing and flowcymetry for ROS levels. Re s ults Any concentration of H2 O2 could do harm to HUVECs co-culturing with cells. Cell viabilities decreased statistically when treating cells with 400, 600 or 800 μmol/L H2 O2 for different times (P<0.05) . Cell viabilities also decreased gradually as time prolonged when treating cells with 800 μmol/L H2 O2 (P<0.001) . When treating cells with different concentration of H2 O2 for 24 h, cell apoptosis rates increased dramatically (1000μmol/L as an exception, P<0.05) , so did cell necrosis rates (100 μmol/L as an exception, P<0.05) ;When treating cells with 800 μmol/L H2 O2, cell apoptosis rates increased dramatically as time prolonged, reaching the peak at 24 h (P<0.05) , so did necrosis rates, reaching the peak at 48 h (P<0.05) . ROS level reached thepeak while treating cells with 400 μmol/L H2 O2 (P<0.001) ; ROS levels increased gradully as time prolonged when treating cells with 800 μmol/L H2 O2 (P<0.001) . Conclusions The oxidative stress model in HUVECs was established by coculturing cells with culture medium containing 800 μmol/L hydrogen peroxide for 24 h.
2018, 39(04): 40-46.
Abstract:
Objective To identify two Fasciola spp., Sample YXGD01 and YXGD02, collected from one female fasciolosis patient living in Yuxin, Yunnan Province in China using DNA marker nad1 and ITS1, and to investigate causative agents of Zoonoses fasciolosis in humans. Me thods Two Fasciola fluckes collected from one female patient were preserved in 75% ethyl alcohol. The mitochondrial NADH dehydrogenase subunit 1 (nad1) gene and first internal transcribed spacer (ITS1) of nuclear ribosomal DNA (r DNA) of individual trematodes were amplified by polymerase chain reaction (PCR) . Then the PCR product were sequenced in forward and reverse direction. The sequences analyzed by comparing with related sequences of Fasciola available in Genbank database.Using the DNA database, to identify Fasciola spp. by haplotype analysis for ITS1 and construct phylogenetic tree by NJ method for nad1. Re s ults 422 bp ITS1 sequences and 542 bp nad1 sequences were obtained respectively for thetwo Fasciola fluckes sequenced in our study. Using haplotype analysis method, both Fasciola fluckes with the identical nad1 haplotype similar toF. gigiantica (Fg) haplogoup, showed Fg type to sample YXGD01 and Fh type to sample YXGD02 in ITS1 respectively. The NJ phylogenetic tree inferred from nad1 displays distinct F. gigantica goup and F. hepatica goup. Conclusion This study identified the two Fasciola spp. from one fasciolosis patient living in Yuxi, Yunnan Province usingmitochondria nad1 and nuclear ITS1. The sample YXGD01 should belong toF.gigantica in molecular characteristics. The confusion result between ITS1 and nad1 haplotype of sample YXGD02 infers that it may belong to one special type of intermediate form Fasciola between F. hepatica and F. gigantica, but more experimental proof is needed.
Objective To identify two Fasciola spp., Sample YXGD01 and YXGD02, collected from one female fasciolosis patient living in Yuxin, Yunnan Province in China using DNA marker nad1 and ITS1, and to investigate causative agents of Zoonoses fasciolosis in humans. Me thods Two Fasciola fluckes collected from one female patient were preserved in 75% ethyl alcohol. The mitochondrial NADH dehydrogenase subunit 1 (nad1) gene and first internal transcribed spacer (ITS1) of nuclear ribosomal DNA (r DNA) of individual trematodes were amplified by polymerase chain reaction (PCR) . Then the PCR product were sequenced in forward and reverse direction. The sequences analyzed by comparing with related sequences of Fasciola available in Genbank database.Using the DNA database, to identify Fasciola spp. by haplotype analysis for ITS1 and construct phylogenetic tree by NJ method for nad1. Re s ults 422 bp ITS1 sequences and 542 bp nad1 sequences were obtained respectively for thetwo Fasciola fluckes sequenced in our study. Using haplotype analysis method, both Fasciola fluckes with the identical nad1 haplotype similar toF. gigiantica (Fg) haplogoup, showed Fg type to sample YXGD01 and Fh type to sample YXGD02 in ITS1 respectively. The NJ phylogenetic tree inferred from nad1 displays distinct F. gigantica goup and F. hepatica goup. Conclusion This study identified the two Fasciola spp. from one fasciolosis patient living in Yuxi, Yunnan Province usingmitochondria nad1 and nuclear ITS1. The sample YXGD01 should belong toF.gigantica in molecular characteristics. The confusion result between ITS1 and nad1 haplotype of sample YXGD02 infers that it may belong to one special type of intermediate form Fasciola between F. hepatica and F. gigantica, but more experimental proof is needed.
2018, 39(04): 47-51.
Abstract:
Objective To investigate the association between single nucleotide polymorphisms (SNPs) in IL1 RN and risk of glioma. Me thods A total of 200 glioma patients and 200 healthy controls were enrolled in Kuangwu Hospital of Tongchuan from January 2012 to December 2016. Blood samples were collected. The genotypes of rs17042888, rs315919, rs928940, rs3181052, rs452204 and rs4252019 were genotyped by Mass ARRAY method, and the association between SNPs with glioma risk was evaluated using SPSS 19.0. Re s ults Comparing the allele frequencies of SNPs, we found the allele "G" of rs315919 and allele "C" of rs4252019 were significantly associated with decreased risk of glioma (rs315919: OR=0.841, 95%CI: 0.712-0.993, P=0.041;rs4252019:OR =0.835, 95% CI: 0.707-0.987, P =0.034) . In the genetic model analysis, we found that under recessive model, carries of G/G genotype of rs315919 have 0.75-fold reduced risk of glioma, compared with carries of T/T and G/T genotypes (OR=0.75, 95%CI: 0.52-0.96, P=0.026) . Under dominant model, carries of C/T and C/C genotypes of rs4252019 have 0.76-fold reduced risk of glioma, compared with carries of T/T genotype (OR=0.76, 95 %CI: 0.59-0.97, P=0.025) . Conclus ion The rs315919 and rs4252019 in IL1 RN were associated with decreased risk of glioma.
Objective To investigate the association between single nucleotide polymorphisms (SNPs) in IL1 RN and risk of glioma. Me thods A total of 200 glioma patients and 200 healthy controls were enrolled in Kuangwu Hospital of Tongchuan from January 2012 to December 2016. Blood samples were collected. The genotypes of rs17042888, rs315919, rs928940, rs3181052, rs452204 and rs4252019 were genotyped by Mass ARRAY method, and the association between SNPs with glioma risk was evaluated using SPSS 19.0. Re s ults Comparing the allele frequencies of SNPs, we found the allele "G" of rs315919 and allele "C" of rs4252019 were significantly associated with decreased risk of glioma (rs315919: OR=0.841, 95%CI: 0.712-0.993, P=0.041;rs4252019:OR =0.835, 95% CI: 0.707-0.987, P =0.034) . In the genetic model analysis, we found that under recessive model, carries of G/G genotype of rs315919 have 0.75-fold reduced risk of glioma, compared with carries of T/T and G/T genotypes (OR=0.75, 95%CI: 0.52-0.96, P=0.026) . Under dominant model, carries of C/T and C/C genotypes of rs4252019 have 0.76-fold reduced risk of glioma, compared with carries of T/T genotype (OR=0.76, 95 %CI: 0.59-0.97, P=0.025) . Conclus ion The rs315919 and rs4252019 in IL1 RN were associated with decreased risk of glioma.
2018, 39(04): 52-55.
Abstract:
Objective To investigate the effect of cinnamaldehyde and vancomycin on Biofilm inhibition of methicillin-resistant staphylococcus aureus (MRSA) . Me thods Modified plate culture method was used to establish in vitro biofilm model for MRSA standard strain ATCC25923 and other 5 strains from patient samples, and crystal violet semi-quantitative method was applied to detect the biofilm formation. The minimum inhibitory concentration (MIC) of ATCCA strain and typical MRSA strain was determined using triphenyl tetrazolium chloride (TTC) method. Micrdilution checkerboard techniques was adopted to determine the MIC of drugs combination. Re s ults The biofilm will be maturated in three days;Strain 16187 showed strong capacity of biomilm formation; MICs of standard strain for cinnamaldehyde and vancomycin were 256 μg/m L and 1 μg/m L; MICs of strain 16187 for cinnamaldehyde and vancomycin were 128 μg/m L and 0.5 μg/m L;MIC of standard strain for combination of cinnamaldehyde and vancomycin were 16 μg/m L and 0.006 μg/m L, and 16 μg/m L and 0.03 μg/m L for stain 16187. Comparing MRSA16187 in treated with medicatoin conbired with cinnamaldehyde and vancomycinand single medication the difference of sMIC50 was significant (P<0.05 and P<0.05) , and there was significant difference when we compared sMIC90 (P<0.05 and P<0.05) ;Comparing ATCC25923 treated with medication combined with cinnamaldehyde and vancomycin the difference was of sMIC50 significant (P<0.05 and P<0.05) , and sMIC90 showed the significant difference (P <0.05 and P <0.01) .Conclusion Cinnamaldehyde synergistically enhances the capacity of vancomycin on biofilminhibitory on MRSA.
Objective To investigate the effect of cinnamaldehyde and vancomycin on Biofilm inhibition of methicillin-resistant staphylococcus aureus (MRSA) . Me thods Modified plate culture method was used to establish in vitro biofilm model for MRSA standard strain ATCC25923 and other 5 strains from patient samples, and crystal violet semi-quantitative method was applied to detect the biofilm formation. The minimum inhibitory concentration (MIC) of ATCCA strain and typical MRSA strain was determined using triphenyl tetrazolium chloride (TTC) method. Micrdilution checkerboard techniques was adopted to determine the MIC of drugs combination. Re s ults The biofilm will be maturated in three days;Strain 16187 showed strong capacity of biomilm formation; MICs of standard strain for cinnamaldehyde and vancomycin were 256 μg/m L and 1 μg/m L; MICs of strain 16187 for cinnamaldehyde and vancomycin were 128 μg/m L and 0.5 μg/m L;MIC of standard strain for combination of cinnamaldehyde and vancomycin were 16 μg/m L and 0.006 μg/m L, and 16 μg/m L and 0.03 μg/m L for stain 16187. Comparing MRSA16187 in treated with medicatoin conbired with cinnamaldehyde and vancomycinand single medication the difference of sMIC50 was significant (P<0.05 and P<0.05) , and there was significant difference when we compared sMIC90 (P<0.05 and P<0.05) ;Comparing ATCC25923 treated with medication combined with cinnamaldehyde and vancomycin the difference was of sMIC50 significant (P<0.05 and P<0.05) , and sMIC90 showed the significant difference (P <0.05 and P <0.01) .Conclusion Cinnamaldehyde synergistically enhances the capacity of vancomycin on biofilminhibitory on MRSA.
2018, 39(04): 62-65.
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Objective To investigate the value of contrast enhanced ultrasonography in catheter drainage to determine the position and patency of catheter. Me thods We made a retrospective analysis in patients (78 cases) received percutaneous ultrasound-guided catheter drainage and diluted ultrasound contrast agents injection, and observed the catheter position and drainage effect. Re s ults Eighty-one drainage catheters developed the whole tube clearly, with catheter tips located at a predetermined position and the contrast agent diffusion region coincided with the predetermined region, accounting for 87.1%. Five catheter tips were unclear, while the contrast agent diffusion region coincided with the predetermined region, accounting for 5.4%. Three catheter tips failed to reach the predetermined position, and four drainage catheters developed part of the body of tubes clearly and showed cut off, accounting for 7.5%. Seven drainage catheter were pulled out and placed again, and then injected the diluted ultrasound contrast agent. In the replaced catheters, the catheters developed the whole tube clearly, catheter tips located at a predetermined position, and the contrast agent diffusion region coincided with the predetermined region.Conclus ion Contrast enhanced ultrasonography by diluted ultrasound contrast agent Sonovue injection through catheter can display drainage catheter, and determine the location of the catheter and drainage area. It is a reliable and non-invasive method and has a high clinical value.
Objective To investigate the value of contrast enhanced ultrasonography in catheter drainage to determine the position and patency of catheter. Me thods We made a retrospective analysis in patients (78 cases) received percutaneous ultrasound-guided catheter drainage and diluted ultrasound contrast agents injection, and observed the catheter position and drainage effect. Re s ults Eighty-one drainage catheters developed the whole tube clearly, with catheter tips located at a predetermined position and the contrast agent diffusion region coincided with the predetermined region, accounting for 87.1%. Five catheter tips were unclear, while the contrast agent diffusion region coincided with the predetermined region, accounting for 5.4%. Three catheter tips failed to reach the predetermined position, and four drainage catheters developed part of the body of tubes clearly and showed cut off, accounting for 7.5%. Seven drainage catheter were pulled out and placed again, and then injected the diluted ultrasound contrast agent. In the replaced catheters, the catheters developed the whole tube clearly, catheter tips located at a predetermined position, and the contrast agent diffusion region coincided with the predetermined region.Conclus ion Contrast enhanced ultrasonography by diluted ultrasound contrast agent Sonovue injection through catheter can display drainage catheter, and determine the location of the catheter and drainage area. It is a reliable and non-invasive method and has a high clinical value.
2018, 39(04): 66-69.
Abstract:
Objective To explore the relationship between the mucosal inflammation and the surgical efficacy of patients with eosinophilic chronic sinusitis. Me thods From September 2014 to September 2017, 30 patients with chronic eosinophils sinusitis were selected in our hospital. We colored the sinus tissue paraffin specimens by HE staining, calculated the number of inflammatory cells by microscope observation and the number of eosinophils contained, determined the eosinophil infiltration degree, evaluated the effect of surgical treatment, and analyzed the eosinophil infiltration degree and the relationship between the clinical classification.Re s ults The infiltration degree of the mucosa related eosinophilic granulocytes was significantly lower (χ2=7.6876, P =0.0055) after1-2 weeks after surgery for patients with chronic sinusitis. The degree of infiltration of the mucosa related eosinophilic granulocytes at 4 weeks and 6 weeks after surgery was 3 and 4. There was no significant difference before surgery (χ2=0.3267, P=0.5676) . The degree of infiltration of the mucosa related eosinophils in the 10-12 weeks after surgery was significantly lower than that in grade 3 and 4 (χ2=13.4842, P=0.0002) . In 30 patients with chronic sinusitis, the treatment efficiency of the mucosa associated eosinophilic granulocytic infiltration level was level 3 and level 4 (χ2= 23.0990, 23.0990, P= 0.0000, 0.0000) . The treatment efficiency of the lesion of the mucosa of patients with chronic sinusitis was level 2 and the level of infiltration was 3 and 4 (χ2=12.2817, 12.2817, P =0.0004, 0.0004) . In the clinical fractal type 1, the degree of infiltration level 1 and 2 was distributed, and the degree of infiltration level 3 and 4 was absent; In the clinical fractal type 2, the degree of infiltration level 1 was mostly observed and the degree of infiltration level 2, 3 and 4 was distributed; In the clinical fractal type 3, the degree of infiltration level 1 was mostly observed and the degree of infiltration level 2, 3 and 4 was distributed. The comparison between infiltration degree level 1 and level 2 of the total clinical classification and the type 2 percentage infiltration degree level 3 and level 4 was not significant (χ2=0.3801, 0.3801 and 0.3801, 0.3801, P=0.5375, 0.5375, 0.5375, 0.5375) . Conclusion There is a certain relationship between the mucosal inflammation and the curative effect of eosinophilic chronic sinusitis.
Objective To explore the relationship between the mucosal inflammation and the surgical efficacy of patients with eosinophilic chronic sinusitis. Me thods From September 2014 to September 2017, 30 patients with chronic eosinophils sinusitis were selected in our hospital. We colored the sinus tissue paraffin specimens by HE staining, calculated the number of inflammatory cells by microscope observation and the number of eosinophils contained, determined the eosinophil infiltration degree, evaluated the effect of surgical treatment, and analyzed the eosinophil infiltration degree and the relationship between the clinical classification.Re s ults The infiltration degree of the mucosa related eosinophilic granulocytes was significantly lower (χ2=7.6876, P =0.0055) after1-2 weeks after surgery for patients with chronic sinusitis. The degree of infiltration of the mucosa related eosinophilic granulocytes at 4 weeks and 6 weeks after surgery was 3 and 4. There was no significant difference before surgery (χ2=0.3267, P=0.5676) . The degree of infiltration of the mucosa related eosinophils in the 10-12 weeks after surgery was significantly lower than that in grade 3 and 4 (χ2=13.4842, P=0.0002) . In 30 patients with chronic sinusitis, the treatment efficiency of the mucosa associated eosinophilic granulocytic infiltration level was level 3 and level 4 (χ2= 23.0990, 23.0990, P= 0.0000, 0.0000) . The treatment efficiency of the lesion of the mucosa of patients with chronic sinusitis was level 2 and the level of infiltration was 3 and 4 (χ2=12.2817, 12.2817, P =0.0004, 0.0004) . In the clinical fractal type 1, the degree of infiltration level 1 and 2 was distributed, and the degree of infiltration level 3 and 4 was absent; In the clinical fractal type 2, the degree of infiltration level 1 was mostly observed and the degree of infiltration level 2, 3 and 4 was distributed; In the clinical fractal type 3, the degree of infiltration level 1 was mostly observed and the degree of infiltration level 2, 3 and 4 was distributed. The comparison between infiltration degree level 1 and level 2 of the total clinical classification and the type 2 percentage infiltration degree level 3 and level 4 was not significant (χ2=0.3801, 0.3801 and 0.3801, 0.3801, P=0.5375, 0.5375, 0.5375, 0.5375) . Conclusion There is a certain relationship between the mucosal inflammation and the curative effect of eosinophilic chronic sinusitis.
2018, 39(04): 70-72.
Abstract:
Objective To study the incidence of pharyngeal fistula and the use of antibiotics in the patients with laryngeal cancer underwent total laryngectomy and to analyze the distribution of bacteria in the operative area of laryngectomy to provide some experimental basis for the use of antibiotics and reduce the incidence of pharyngeal fistula. Me thods A retrospective statistical analysis was conducted involving 42 cases of pharyngeal fistula occurred in 185 patients with laryngeal cancer in the First Affiliated Hospital of Kunming Medical University. Re s ults (1) Among the 185 patients with total laryngectomy, pharyngeal fistula occurred in 42 cases, and the incidence of pharyngeal fistula was 22.70%. Compared with the postoperative antibiotic use and the incidence of pharyngeal fistula (P = 0.005) , the difference was statistically significant. Compared with the use of anti-anaerobes after surgery and the incidence the difference was not statistically significant of hypo-pharyngeal fistula (P = 0.961) , 2) The airway secretions were cultured in 60 patients with laryngectomy, and the most pathogenic bacteria were gram-negative bacteria (67.24%) . Among them, pseudomonas aeruginosa (14 cases, 24.14 %) was the most. Conclus ion The bacteria of postoperative airway fistula after laryngeal cancer was mostly gram-negative, and selecting antibiotics correctly according to the distribution of pathogens in the operative area can significantly reduce the incidence of pharyngeal fistula.
Objective To study the incidence of pharyngeal fistula and the use of antibiotics in the patients with laryngeal cancer underwent total laryngectomy and to analyze the distribution of bacteria in the operative area of laryngectomy to provide some experimental basis for the use of antibiotics and reduce the incidence of pharyngeal fistula. Me thods A retrospective statistical analysis was conducted involving 42 cases of pharyngeal fistula occurred in 185 patients with laryngeal cancer in the First Affiliated Hospital of Kunming Medical University. Re s ults (1) Among the 185 patients with total laryngectomy, pharyngeal fistula occurred in 42 cases, and the incidence of pharyngeal fistula was 22.70%. Compared with the postoperative antibiotic use and the incidence of pharyngeal fistula (P = 0.005) , the difference was statistically significant. Compared with the use of anti-anaerobes after surgery and the incidence the difference was not statistically significant of hypo-pharyngeal fistula (P = 0.961) , 2) The airway secretions were cultured in 60 patients with laryngectomy, and the most pathogenic bacteria were gram-negative bacteria (67.24%) . Among them, pseudomonas aeruginosa (14 cases, 24.14 %) was the most. Conclus ion The bacteria of postoperative airway fistula after laryngeal cancer was mostly gram-negative, and selecting antibiotics correctly according to the distribution of pathogens in the operative area can significantly reduce the incidence of pharyngeal fistula.
2018, 39(04): 79-82.
Abstract:
Objective To investigate the differential diagnostic value of the Apparent Diffusion Coefficient in Interstitial Edema Pancreatitis (IEP) and Acute Necrotizing Pancreatitis (ANP) . Me thods Twenty-four ANP patients and 53 IEP patients received routine MRI and functional imaging DWI (b value is 0, 800 s/mm2) scanning were retrospectively analyzed. We measured the ADC values of ANP edema, hemorrhage, necrosis areas and the ADC values of the IEP between January 2017 and July 2017. Statistical methods were used to compare and analyze the ADC values of ANP edema, hemorrhage, necrosis areas and the ADC values of the IEP. Re s ults The ADC values of the IEP were (1.094±0.170) ×10-3 mm2/s, and the values of ANP edema, hemorrhage, necrosis areas were (1.099±0.127) ×10-3 mm2/s, (0.584±0.061) ×10-3 mm2/s, (2.084±0.323) ×10-3 mm2/s.There was no statistically significant difference in ADC values in edema area between IEP and ANP (P = 0.884) .Single factor analysis of variance and multiple comparison was used to identify the ADC values of IEP and the values of ANP edema, hemorrhage, and necrosis areas. The results were statistically significant (P < 0.001) . Conclus ion The ADC values of IEP and ANP edema zone have no obvious difference. The ADC values of ANP hemorrhage area are significantly lower than those of edema area and IEP. The ADC values of ANP necrotic area are significantly higher than those of edema and hemorrhage area. ADC values have important value in identifying IEP and ANP.
Objective To investigate the differential diagnostic value of the Apparent Diffusion Coefficient in Interstitial Edema Pancreatitis (IEP) and Acute Necrotizing Pancreatitis (ANP) . Me thods Twenty-four ANP patients and 53 IEP patients received routine MRI and functional imaging DWI (b value is 0, 800 s/mm2) scanning were retrospectively analyzed. We measured the ADC values of ANP edema, hemorrhage, necrosis areas and the ADC values of the IEP between January 2017 and July 2017. Statistical methods were used to compare and analyze the ADC values of ANP edema, hemorrhage, necrosis areas and the ADC values of the IEP. Re s ults The ADC values of the IEP were (1.094±0.170) ×10-3 mm2/s, and the values of ANP edema, hemorrhage, necrosis areas were (1.099±0.127) ×10-3 mm2/s, (0.584±0.061) ×10-3 mm2/s, (2.084±0.323) ×10-3 mm2/s.There was no statistically significant difference in ADC values in edema area between IEP and ANP (P = 0.884) .Single factor analysis of variance and multiple comparison was used to identify the ADC values of IEP and the values of ANP edema, hemorrhage, and necrosis areas. The results were statistically significant (P < 0.001) . Conclus ion The ADC values of IEP and ANP edema zone have no obvious difference. The ADC values of ANP hemorrhage area are significantly lower than those of edema area and IEP. The ADC values of ANP necrotic area are significantly higher than those of edema and hemorrhage area. ADC values have important value in identifying IEP and ANP.
2018, 39(04): 83-87.
Abstract:
Objective To analyze the efficacy and safety of different surgical procedures in the treatment of suppurative encapsulated pleural effusion. Me thods Sixty-eight patients with suppurative pleural effusion were randomly divided into group A and group B, with 34 cases in each group. The two groups of patients were treated with thoracoscopic small incision thoracoscopic clearance and fibreboard exfoliation. In group A, antibiotics were used to rinse the chest. Group B was placed with lateral venous catheter during operation. Then we used antibacterial agents to irrigate the thoracic cavity. The clinical efficacy, complications, removal time of venous catheters, white blood cell count (WBC) , glutamic acid (Glu) , lactate dehydrogenase (LDH) level, and respiratory function index (FVC, FEV1) in intravenous catheter fluid, pleural thickness, and recurrence rate after oneyear were compared between the two groups.Results The clinical total effective rate of group A was significantly lower than that in group B. Intravenous catheter removal time was significantly longer than that in group B. WBC and Glu after 3 d were significantly higher than those of group B. LDH was significantly lower than that of group B. FVC and FEV1 after the extraction of venous catheter were significantly lower than those in group B. Pleural thickness was higher in group B. The rate of recurrence after one year was higher than that in group B. Conclusion Thoracoscopic small incision thoracic dissection and fibrinectomy and postoperative antibiotics flushing the thoracic cavity can improve the clinical efficacy, but at the same time it increases the economic burden on the patient and should be personalized for clinical use.
Objective To analyze the efficacy and safety of different surgical procedures in the treatment of suppurative encapsulated pleural effusion. Me thods Sixty-eight patients with suppurative pleural effusion were randomly divided into group A and group B, with 34 cases in each group. The two groups of patients were treated with thoracoscopic small incision thoracoscopic clearance and fibreboard exfoliation. In group A, antibiotics were used to rinse the chest. Group B was placed with lateral venous catheter during operation. Then we used antibacterial agents to irrigate the thoracic cavity. The clinical efficacy, complications, removal time of venous catheters, white blood cell count (WBC) , glutamic acid (Glu) , lactate dehydrogenase (LDH) level, and respiratory function index (FVC, FEV1) in intravenous catheter fluid, pleural thickness, and recurrence rate after oneyear were compared between the two groups.Results The clinical total effective rate of group A was significantly lower than that in group B. Intravenous catheter removal time was significantly longer than that in group B. WBC and Glu after 3 d were significantly higher than those of group B. LDH was significantly lower than that of group B. FVC and FEV1 after the extraction of venous catheter were significantly lower than those in group B. Pleural thickness was higher in group B. The rate of recurrence after one year was higher than that in group B. Conclusion Thoracoscopic small incision thoracic dissection and fibrinectomy and postoperative antibiotics flushing the thoracic cavity can improve the clinical efficacy, but at the same time it increases the economic burden on the patient and should be personalized for clinical use.
2018, 39(04): 88-94.
Abstract:
Objective To investigate the association of Vitamin D receptor gene Apa I, Fok I, Taq I, Tru9 I single nucleotide polymorphism with hashimoto's thyroiditis (HT) . Me thods The single nucleotide polymorphism of VDR gene in 139 (HT group) and 66 normal controls (NT group) were detected by Taq Man fluorescent probe.The genotype frequency and allele frequency and relative clinical data were compared between groups. Re s ults (1) Mean serum 25 (OH) D in HT patients in Gejiu was (18.45±7.60) ng/m L. (2) The level of 25 (OH) D in HT [ (17.66 ±7.91) ng/ml] was significantly lower than that in NT group [ (20.13 ±6.65) ng/m L].25 (OH) D deficiency in NT group (42.42%) was significantly higher than that in HT group (22.30%, P = 0.003) .25 (OH) D deficiency in HT group (70.51%) was significantly higher than that in HT group (51.52%, P = 0.008) . (3) Apa I CC, CA and AA genotype frequencies were 0.51, 0.40 and 0.09 and allele C and allele A frequencies were 0.71 and 0.29;Fok I CC, TC and TT genotype frequencies were 0.28, 0.50 and 0.22 and allele T and allele C frequencies were 0.47 and 0.53;Tru9 I GG, GA and AA genotype frequencies were 0.61, 0.33 and 0.06, and allele G and allele A frequencies were 0.77 and 0.23; The genotype frequency and allele frequency in two groups failed to show statistically significance (P<0.05) (4) According to the analysis of logistic regression, 25 (OH) D insufficient was the risk factor related to the occurrence of HT (OR=1.429, P = 0.033) .Conclus ion People with 25 (OH) D deficiency and insufficiency were widespread in Gejiu. 25 (OH) D deficiency in HT group was significantly higher than that in NT group. 25 (OH) D insufficiency may be the independent risk factor related to the occurrence of HT. VDR Apa I, Taq I, Fok I and Tru9 I single nucleotide polymorphism were not related to HT.
Objective To investigate the association of Vitamin D receptor gene Apa I, Fok I, Taq I, Tru9 I single nucleotide polymorphism with hashimoto's thyroiditis (HT) . Me thods The single nucleotide polymorphism of VDR gene in 139 (HT group) and 66 normal controls (NT group) were detected by Taq Man fluorescent probe.The genotype frequency and allele frequency and relative clinical data were compared between groups. Re s ults (1) Mean serum 25 (OH) D in HT patients in Gejiu was (18.45±7.60) ng/m L. (2) The level of 25 (OH) D in HT [ (17.66 ±7.91) ng/ml] was significantly lower than that in NT group [ (20.13 ±6.65) ng/m L].25 (OH) D deficiency in NT group (42.42%) was significantly higher than that in HT group (22.30%, P = 0.003) .25 (OH) D deficiency in HT group (70.51%) was significantly higher than that in HT group (51.52%, P = 0.008) . (3) Apa I CC, CA and AA genotype frequencies were 0.51, 0.40 and 0.09 and allele C and allele A frequencies were 0.71 and 0.29;Fok I CC, TC and TT genotype frequencies were 0.28, 0.50 and 0.22 and allele T and allele C frequencies were 0.47 and 0.53;Tru9 I GG, GA and AA genotype frequencies were 0.61, 0.33 and 0.06, and allele G and allele A frequencies were 0.77 and 0.23; The genotype frequency and allele frequency in two groups failed to show statistically significance (P<0.05) (4) According to the analysis of logistic regression, 25 (OH) D insufficient was the risk factor related to the occurrence of HT (OR=1.429, P = 0.033) .Conclus ion People with 25 (OH) D deficiency and insufficiency were widespread in Gejiu. 25 (OH) D deficiency in HT group was significantly higher than that in NT group. 25 (OH) D insufficiency may be the independent risk factor related to the occurrence of HT. VDR Apa I, Taq I, Fok I and Tru9 I single nucleotide polymorphism were not related to HT.
2018, 39(04): 95-99.
Abstract:
Objective Through the comprehensive geriatric assessment, the incidence of anxiety and related risk factors of the elderly hypertensive patients in the general hospital are understood and the basis for clinical intervention is provided.Me thods We randomly selected the patients with primary hypertension diagnosed in the Department of Geriatrics in our hospital as the research objects. The elderly patients were evaluated comprehensively.The Hamilton Anxiety Scale (HAMA) was used to assess the incidence of anxiety in hypertensive patients and to identify the related risk factors.Re s ults A total number of 317 elderly hypertensive patients were enrolled in this study. The age was 6096 years old. The average age was (82.6 ± 6.4) years old. Among all the participants, 173 were men (54.6%) were male and 144 were women (45.4%) were female.The incidence of anxiety mong all pecrticipants was 27.1% (86/317) , with 10.7% (34/317) in male and 16.4% (52/317) in femde.The independent risk factors for hypertension and anxiety in the elderly were 24 hours average blood pressure, disability and insomnia (P<0.05) .Conclus ions Multiple factors can effect anxiety symptoms of inpatients.Using CGA can detect potential problems in early stage, and develop personalized intervention measures to improve the quality of life of the elderly.
Objective Through the comprehensive geriatric assessment, the incidence of anxiety and related risk factors of the elderly hypertensive patients in the general hospital are understood and the basis for clinical intervention is provided.Me thods We randomly selected the patients with primary hypertension diagnosed in the Department of Geriatrics in our hospital as the research objects. The elderly patients were evaluated comprehensively.The Hamilton Anxiety Scale (HAMA) was used to assess the incidence of anxiety in hypertensive patients and to identify the related risk factors.Re s ults A total number of 317 elderly hypertensive patients were enrolled in this study. The age was 6096 years old. The average age was (82.6 ± 6.4) years old. Among all the participants, 173 were men (54.6%) were male and 144 were women (45.4%) were female.The incidence of anxiety mong all pecrticipants was 27.1% (86/317) , with 10.7% (34/317) in male and 16.4% (52/317) in femde.The independent risk factors for hypertension and anxiety in the elderly were 24 hours average blood pressure, disability and insomnia (P<0.05) .Conclus ions Multiple factors can effect anxiety symptoms of inpatients.Using CGA can detect potential problems in early stage, and develop personalized intervention measures to improve the quality of life of the elderly.
2018, 39(04): 100-103.
Abstract:
Objective To observe the incidence of secondary epilepsy in patients with cerebral infarction and explore the risk factors of secondary epilepsy. Me thods Eighty-four patients with secondary epilepsy diagnosed as cerebral infarction from April 2016 to August 2017 in our hospital were selected as the observation group, and 84 patients of cerebral infarction without epilepsy in the same period were selected as the control group. Follow-up of secondary epileptic seizures, the general information of patients, basic diseases, hypertension grade, blood electrolytes, lesion location, diameter of lesion were recorded, and the risk factors of secondary epilepsy in cerebral infarction were screened, and the status of secondary epilepsy were analyzed. Re s ults (1) The types of epilepsy in secondary epilepsy: partial seizure in 69 cases, complete attack in 15 cases; onset time: early onset in 60 cases, late in 24 cases. (2) There was no significant difference between the two groups in general information, underlying disease, lesion diameter and grade ofhypertension (P>0.05) . The differences statistically significento significance in the consist of lesion location and lesion specific location and blood electrolyte levels (P <0.05) .Unconditional multivariate logistic regression analysis showed thoao infarction involving the cortex, temporal lobe, and serum chloride value was correlated with the occurrence of secondary epilepsy (P<0.05) . (3) MultivariateLogistic regression analysis showed tthat infaretion involving the cortex (OR = 3.10, P = 0.004, 95% CI =1.52-7.41) , locating in the temporal lobe, (OR = 2.98, P = 0.012, 95% CI = 1.32-7.35) and serum chlorine (OR = 1.25, P = 0.017, 95% CI = 1.00-1.38) were the risk factors for secondary epilepsy in cerebral infarction.Conclusions Partial and early onset of seizures are the most common in cerebral infarction secondary epilepsy.Infarction involving the cortex, locating in the temporal lobe and serum chlorine are the risk factors for secondary epilepsy in patients with cerebral infarction. Necessary measures should be taken to intervene at high risk of secondary epilepsy and to improve the clinical quality of life of patients.
Objective To observe the incidence of secondary epilepsy in patients with cerebral infarction and explore the risk factors of secondary epilepsy. Me thods Eighty-four patients with secondary epilepsy diagnosed as cerebral infarction from April 2016 to August 2017 in our hospital were selected as the observation group, and 84 patients of cerebral infarction without epilepsy in the same period were selected as the control group. Follow-up of secondary epileptic seizures, the general information of patients, basic diseases, hypertension grade, blood electrolytes, lesion location, diameter of lesion were recorded, and the risk factors of secondary epilepsy in cerebral infarction were screened, and the status of secondary epilepsy were analyzed. Re s ults (1) The types of epilepsy in secondary epilepsy: partial seizure in 69 cases, complete attack in 15 cases; onset time: early onset in 60 cases, late in 24 cases. (2) There was no significant difference between the two groups in general information, underlying disease, lesion diameter and grade ofhypertension (P>0.05) . The differences statistically significento significance in the consist of lesion location and lesion specific location and blood electrolyte levels (P <0.05) .Unconditional multivariate logistic regression analysis showed thoao infarction involving the cortex, temporal lobe, and serum chloride value was correlated with the occurrence of secondary epilepsy (P<0.05) . (3) MultivariateLogistic regression analysis showed tthat infaretion involving the cortex (OR = 3.10, P = 0.004, 95% CI =1.52-7.41) , locating in the temporal lobe, (OR = 2.98, P = 0.012, 95% CI = 1.32-7.35) and serum chlorine (OR = 1.25, P = 0.017, 95% CI = 1.00-1.38) were the risk factors for secondary epilepsy in cerebral infarction.Conclusions Partial and early onset of seizures are the most common in cerebral infarction secondary epilepsy.Infarction involving the cortex, locating in the temporal lobe and serum chlorine are the risk factors for secondary epilepsy in patients with cerebral infarction. Necessary measures should be taken to intervene at high risk of secondary epilepsy and to improve the clinical quality of life of patients.
2018, 39(04): 104-108.
Abstract:
目的 探究幽门螺杆菌 (H.pylori) 对不同种抗菌药物耐药问题的研究.方法 选取云南省昆明市第一人民医院2015年8月至2017年4月接诊出现消化道症状且经胃镜检查确诊幽门螺杆菌感染的240例患者为研究对象, 按照临床给药不同分为A、B、C 3组, A组80例患者给予 (埃索美拉唑+甲硝唑+左氧氟沙星) 治疗, B组80例患者给予 (埃索美拉唑+克拉霉素+左氧氟沙星) 治疗, C组80例患者给予 (埃索美拉唑+阿莫西林+左氧氟沙星) 治疗, 3组患者治疗10 d后通过胃镜从患者胃窦取2块胃粘膜组织, 进行幽门螺杆菌培养及E-test测试, 观察幽门螺杆菌感染个体对不同种抗菌药物的耐药性、临床治疗效果以及不良反应发生情况.结果 A组甲硝唑耐药率为51.25% (54/80) , 明显高于B组、C组, C组临床治疗总有效率为93.50%, 明显高于B组与A组, C组不良反应发生率为3.75%, 明显低于B组、A组, 各项数据差异有统计学意义 (P<0.05) .结论 清除幽门螺杆菌治疗中, 甲硝唑耐药性较高, 阿莫西林耐药性最低.
2018, 39(04): 109-112.
Abstract:
Objective To evaluate the role of lung ultrasonography (LUS) in the diagnosis of neonatal pneumonia in plateau area. Me thods A total number of 200 cases of clinically diagnosed neonatal pneumonia were examined by bedside lung ultrasound from June 2015 to December 2017 and 200 cases of icterus neonatorum were selected as the control group. The chest wall was divided into 6 regions by side-sternal line, anterior axillary line and posterior axillary line. Each region was scanned by ultrasound horizontally and longitudinally. Pleural line, A-line, B-line, pulmonary consolidation and pleural effusion were checked. Re s ults The positive rate of bedside lung ultrasound examination in 200 cases of neonatal pneumonia was 100%. The main ultrasonographic manifestations of neonatal pneumonia were as follows: pulmonary consolidation (69/200 cases, 34.5%) , multiple B-lines in a multi regions (200/200 cases, 100%) , pleural line abnormalities (22/200 cases, 11%) , and localized pleural effusion (13/200 cases, 6.5%) . Only A-line (169/200 cases, 84.5%) and B-line (31/200 cases, 15.5%) were observed in the control group. There were statistical differences between the two groups (χ2= 13.65, P<0.05) . Conclus ion The main ultrasonographic manifestations of neonatal pneumonia in plateau area included B-lines, pulmonary consolidation, pleural line abnormalities and localized pleural effusion. As the highest occurrence rate of B-line and pulmonary consolidation, they can be used as a reliable sign for diagnosis of neonatal pneumonia.
Objective To evaluate the role of lung ultrasonography (LUS) in the diagnosis of neonatal pneumonia in plateau area. Me thods A total number of 200 cases of clinically diagnosed neonatal pneumonia were examined by bedside lung ultrasound from June 2015 to December 2017 and 200 cases of icterus neonatorum were selected as the control group. The chest wall was divided into 6 regions by side-sternal line, anterior axillary line and posterior axillary line. Each region was scanned by ultrasound horizontally and longitudinally. Pleural line, A-line, B-line, pulmonary consolidation and pleural effusion were checked. Re s ults The positive rate of bedside lung ultrasound examination in 200 cases of neonatal pneumonia was 100%. The main ultrasonographic manifestations of neonatal pneumonia were as follows: pulmonary consolidation (69/200 cases, 34.5%) , multiple B-lines in a multi regions (200/200 cases, 100%) , pleural line abnormalities (22/200 cases, 11%) , and localized pleural effusion (13/200 cases, 6.5%) . Only A-line (169/200 cases, 84.5%) and B-line (31/200 cases, 15.5%) were observed in the control group. There were statistical differences between the two groups (χ2= 13.65, P<0.05) . Conclus ion The main ultrasonographic manifestations of neonatal pneumonia in plateau area included B-lines, pulmonary consolidation, pleural line abnormalities and localized pleural effusion. As the highest occurrence rate of B-line and pulmonary consolidation, they can be used as a reliable sign for diagnosis of neonatal pneumonia.
2018, 39(04): 113-116.
Abstract:
Objective To summarize the characteristics of PPP disease complex with placenta implication and explore the value of prenatal ultrasonographic scoring system in placenta prevlia complicate with placenta accrete.Me thods An retrospective analysis was conducted among 179 case of ultrasound images of pernicious placenta previa complicate with placenta accrete, and the results of postpartum were compared. Based on clinical surgery and/or pathological results as the gold standard, all cases were divided into adhesive placenta accreta group and non-adhesive placenta accreta group. Re s ults Logistic regression identified six sonographic parameters, including placenta thickness, obliteration of retroplacental hypoechoic space, smallest sagittal myometrial thickness, presence of abnormal vessels linking the placenta to the bladder, lacunar flow distributed within intraparenchymal placenta area and the cervical form. Different scores were assigned according to the odds ratio of these parameters.The critical score of the ultrasound score of adhesive placenta accreta and non-adhesive placenta was 6 points.Conclus ions Prenatal ultrasonographic scoring system is a reliable method to diagnose the degree of the accretion in pernicious placenta previa complicate with placenta accrete.
Objective To summarize the characteristics of PPP disease complex with placenta implication and explore the value of prenatal ultrasonographic scoring system in placenta prevlia complicate with placenta accrete.Me thods An retrospective analysis was conducted among 179 case of ultrasound images of pernicious placenta previa complicate with placenta accrete, and the results of postpartum were compared. Based on clinical surgery and/or pathological results as the gold standard, all cases were divided into adhesive placenta accreta group and non-adhesive placenta accreta group. Re s ults Logistic regression identified six sonographic parameters, including placenta thickness, obliteration of retroplacental hypoechoic space, smallest sagittal myometrial thickness, presence of abnormal vessels linking the placenta to the bladder, lacunar flow distributed within intraparenchymal placenta area and the cervical form. Different scores were assigned according to the odds ratio of these parameters.The critical score of the ultrasound score of adhesive placenta accreta and non-adhesive placenta was 6 points.Conclus ions Prenatal ultrasonographic scoring system is a reliable method to diagnose the degree of the accretion in pernicious placenta previa complicate with placenta accrete.
2018, 39(04): 117-120.
Abstract:
Objective To investigate the effects of amniotic fluid meconium contamination and timely termination of pregnancy on perinatal outcome of intrahepatic cholestasis of pregnancy (ICP) . Me thods From December 2012 to December 2015, 208 cases of ICP were selected in the Department of Obstetrics of the First People's Hospital of Zhaotong City, Yunnan Province. The retrospective study included two groups: the experimental group with 208 cases (ICP group) and the control group with10890 normal deliveries (non-ICP group) . They were treated as follows: (1) fetal monitoring; (2) tests of liver function, bile acid and coagulation; (3) selective amniocentesis or artificial membrane ruptured amniotic fluid traits; (4) determination of the timing of the termination; (5) the choice of gestation mode and the control of the amniotic fluid meconium contamination rate, premature birth rate and perinatal mortality. Re s ults Sixty-three cases (30.29%) of amniotic fluid meconium were found in ICP group including 5 cases of amniocentesis, 45 cases of artificial rupture of membranes, 10 cases of cesarean section and 3 cases of natural rupture. Preterm birth was 50 cases (24.04%) and cesarean section was120 cases (57.69%) . In the control group, there were 814 cases of amniotic fluid meconium (7.41%) , 1214 cases of preterm birth (11.15%) and 3424 cases (31.44%) of cesarean section. The rates of amniotic fluid infection, preterm birth and cesarean section in ICP patients were significantly higher than those in the control group (P <0.05) . Conclusion Important clinical value is observed in early detection of amniotic fluid meconium contamination and timely termination of pregnancy to reduce perinatal mortality among ICP patients.
Objective To investigate the effects of amniotic fluid meconium contamination and timely termination of pregnancy on perinatal outcome of intrahepatic cholestasis of pregnancy (ICP) . Me thods From December 2012 to December 2015, 208 cases of ICP were selected in the Department of Obstetrics of the First People's Hospital of Zhaotong City, Yunnan Province. The retrospective study included two groups: the experimental group with 208 cases (ICP group) and the control group with10890 normal deliveries (non-ICP group) . They were treated as follows: (1) fetal monitoring; (2) tests of liver function, bile acid and coagulation; (3) selective amniocentesis or artificial membrane ruptured amniotic fluid traits; (4) determination of the timing of the termination; (5) the choice of gestation mode and the control of the amniotic fluid meconium contamination rate, premature birth rate and perinatal mortality. Re s ults Sixty-three cases (30.29%) of amniotic fluid meconium were found in ICP group including 5 cases of amniocentesis, 45 cases of artificial rupture of membranes, 10 cases of cesarean section and 3 cases of natural rupture. Preterm birth was 50 cases (24.04%) and cesarean section was120 cases (57.69%) . In the control group, there were 814 cases of amniotic fluid meconium (7.41%) , 1214 cases of preterm birth (11.15%) and 3424 cases (31.44%) of cesarean section. The rates of amniotic fluid infection, preterm birth and cesarean section in ICP patients were significantly higher than those in the control group (P <0.05) . Conclusion Important clinical value is observed in early detection of amniotic fluid meconium contamination and timely termination of pregnancy to reduce perinatal mortality among ICP patients.
2018, 39(04): 121-124.
Abstract:
Objective To investigate the cause of dropout among people living with HIV/AIDS receiving free antiretroviral therapy (ART) in Yunnan province and its associated factors. Me thods Data of patients with HIV/AIDS receiving ART were collected from ART initiative in July 2004 to May 2015 in four counties of Yunnan Province. The possible factors influencing the patients' dropout during the treatment were analyzed by χ2 test and logistic regression analysis. Re s ults Among 6614 patients with HIV/AIDS receiving antiretroviral therapy older than 15 years old, 540 patients dropped out of ART for various reasons, accounting for 8.2%. Among 540 patients who dropped out from ART, 74.4% were lost to follow-up and 25.6% quitted ART but could be reached by health workers. The main factors of dropout during the ART included the baseline CD4 count above 500, being non-marital, intravenous drug using, and receiving treatment for less than 6 months. Conclus ion We should pay attention to patients addicted to drug, receiving treatment less than 6 months, being non-marital or divorced and with baseline CD4 count above 500 in order to reduce the dropout rate by strengthening compliance and giving more care.
Objective To investigate the cause of dropout among people living with HIV/AIDS receiving free antiretroviral therapy (ART) in Yunnan province and its associated factors. Me thods Data of patients with HIV/AIDS receiving ART were collected from ART initiative in July 2004 to May 2015 in four counties of Yunnan Province. The possible factors influencing the patients' dropout during the treatment were analyzed by χ2 test and logistic regression analysis. Re s ults Among 6614 patients with HIV/AIDS receiving antiretroviral therapy older than 15 years old, 540 patients dropped out of ART for various reasons, accounting for 8.2%. Among 540 patients who dropped out from ART, 74.4% were lost to follow-up and 25.6% quitted ART but could be reached by health workers. The main factors of dropout during the ART included the baseline CD4 count above 500, being non-marital, intravenous drug using, and receiving treatment for less than 6 months. Conclus ion We should pay attention to patients addicted to drug, receiving treatment less than 6 months, being non-marital or divorced and with baseline CD4 count above 500 in order to reduce the dropout rate by strengthening compliance and giving more care.
2018, 39(04): 125-129.
Abstract:
Objective To explore the preventive measures through the analysis of the influential factors and the characteristics of human wounded based on the AIS (abbreviated injury scale) score to the occupant of the coach happened head-on collision. Me thods We adopted the accident investigation in-depth method and conducted statistical analysis and regression analysis of human wounded and related factors. Re s ults The person's gender had no significant difference on the AIS. The main body parts wounded were the head (AIS mean =3.7647) and the chest (AIS mean =4.000) , but no significant differences was shown in the t-test (t =-0.275, sig =0.785>0.05) ; The possibility of injury happened in the front three rows (AIS=4.750) was significantly higher than that of the average injury (AIS=1.706 (t=-4.819, Sig=0.000<0.05) happened in other rows; The seat fixed firmly with seatbelt can significantly reduce the occupant hurt. An analysis of variance showed that age, the seat row and the seat belt were the significant relative factors to the occupant hurt. The binomial logistic regression model showed that the death to the non-death probability ratio of the front two rows was up to 3 to 11 times.Conclus ion The significant factors affecting the degree of hurt are age, the seat rows and seat belt. The death to non-death probability ratio of the front two rows is too high, and the lap belt are not enough to protect the head and chest of the passengers. The front structure of the coach should be improved for the safety and the use of seatbelt should be encouraged while travelling.
Objective To explore the preventive measures through the analysis of the influential factors and the characteristics of human wounded based on the AIS (abbreviated injury scale) score to the occupant of the coach happened head-on collision. Me thods We adopted the accident investigation in-depth method and conducted statistical analysis and regression analysis of human wounded and related factors. Re s ults The person's gender had no significant difference on the AIS. The main body parts wounded were the head (AIS mean =3.7647) and the chest (AIS mean =4.000) , but no significant differences was shown in the t-test (t =-0.275, sig =0.785>0.05) ; The possibility of injury happened in the front three rows (AIS=4.750) was significantly higher than that of the average injury (AIS=1.706 (t=-4.819, Sig=0.000<0.05) happened in other rows; The seat fixed firmly with seatbelt can significantly reduce the occupant hurt. An analysis of variance showed that age, the seat row and the seat belt were the significant relative factors to the occupant hurt. The binomial logistic regression model showed that the death to the non-death probability ratio of the front two rows was up to 3 to 11 times.Conclus ion The significant factors affecting the degree of hurt are age, the seat rows and seat belt. The death to non-death probability ratio of the front two rows is too high, and the lap belt are not enough to protect the head and chest of the passengers. The front structure of the coach should be improved for the safety and the use of seatbelt should be encouraged while travelling.
2018, 39(04): 130-134.
Abstract:
糖尿病是临床常见慢性代谢性疾病, 是失明、肾衰竭、心脏病发作、中风和下肢截肢的主要病因, 死亡率逐年增加, 据预测, 2030年, 糖尿病将成为第7主要死因.因此, 糖尿病的预防及治疗至关重要.近年来, 国内外相关研究表明, 通过开展糖尿病健康教育, 可以让患者及家属、高危人群掌握糖尿病的防治知识, 提高自我保健意识, 提高现症患者就诊率, 减少、延缓并发症的发生, 延长寿命, 降低糖尿病病死率, 同时提高糖尿病患者的生活质量, 健康教育作为糖尿病综合防治5项措施之一, 在糖尿病防治中发挥了重要作用.就糖尿病健康教育的对象、内容、作用、模式进行综述.
2018, 39(04): 135-140.
Abstract:
Lynch syndrome (LS) is the most common hereditary colorectal cancer syndrome, an autosomal dominant inherited disease which is caused by mismatch repair system defect. Learning knowledge in the genetics and diagnosis of LS, and identifying at-risk patients and making a correct diagnosis are the keys to successful screening and surgical decision-making and chemoprevention which is important to decrease formation of and death from cancers. This article reviews recent developments in mainly the clinical features, genetics, current diagnostic standard and diagnosis, surveillance, management, treatment and other related syndromes of LS.
Lynch syndrome (LS) is the most common hereditary colorectal cancer syndrome, an autosomal dominant inherited disease which is caused by mismatch repair system defect. Learning knowledge in the genetics and diagnosis of LS, and identifying at-risk patients and making a correct diagnosis are the keys to successful screening and surgical decision-making and chemoprevention which is important to decrease formation of and death from cancers. This article reviews recent developments in mainly the clinical features, genetics, current diagnostic standard and diagnosis, surveillance, management, treatment and other related syndromes of LS.
2018, 39(04): 141-144.
Abstract:
Objective To explore the feasibility and function in improving the information literacy of medical students by using the modularization and project-driven teaching in the teaching of Medical Information Retrieval.Me thods A total number of 197 students in four classes majoring in clinical medicine were randomly allocated into the experimental group (98 students) and the control group (99 students) . All classes were taught by one teacher, who applied modularization and project-driven teaching in the experimental group and traditional teaching in the control group. Two groups took the same exam at the same time, and test scores were analyzed. Re s ults The scores of experimental group were significantly higher than those of the control group (P < 0.05) . Conclus ions Modularization and project-driven teaching can mobilize students' internal cause for learning and cultivate consciousness of information, the ability of autonomous learning, independent thinking and teamwork, which shows the positive effect for improving students' information literacy.
Objective To explore the feasibility and function in improving the information literacy of medical students by using the modularization and project-driven teaching in the teaching of Medical Information Retrieval.Me thods A total number of 197 students in four classes majoring in clinical medicine were randomly allocated into the experimental group (98 students) and the control group (99 students) . All classes were taught by one teacher, who applied modularization and project-driven teaching in the experimental group and traditional teaching in the control group. Two groups took the same exam at the same time, and test scores were analyzed. Re s ults The scores of experimental group were significantly higher than those of the control group (P < 0.05) . Conclus ions Modularization and project-driven teaching can mobilize students' internal cause for learning and cultivate consciousness of information, the ability of autonomous learning, independent thinking and teamwork, which shows the positive effect for improving students' information literacy.
2018, 39(04): 145-148.
Abstract:
Objective To explore the application of Bloom's Taxonomy of Educational Objectives in the Department of Gynecology in the theoretical teaching. Me thods The research subjects were 119 medical students in Kunming Medical University from November 2017 to December 2017 enrolled in the theoretical course of obstetrics and gynecology. In the experimental group, 53 medical students were taught by Bloom's Taxonomy of Educational Objectives. The traditional mode of teaching was adopted among 63 medical students in the control group. Students took the after-class test and the scores were compared using normal distribution detection and independent sample t-test. Re s ults At the end of the course, the scores of the experimental group and the control group were all in line with the normal distribution P = 0.641 and P = 0.098 (> 0.05) , and the average score of the experimental group was significantly higher than that of the control group P = 0.00 (<0.01) . Conclus ion The taxonomy of Bloom's Taxonomy of Educational Objectives should be used in the teaching of the theory course of obstetrics and gynecology, which can improve the quality of teaching.
Objective To explore the application of Bloom's Taxonomy of Educational Objectives in the Department of Gynecology in the theoretical teaching. Me thods The research subjects were 119 medical students in Kunming Medical University from November 2017 to December 2017 enrolled in the theoretical course of obstetrics and gynecology. In the experimental group, 53 medical students were taught by Bloom's Taxonomy of Educational Objectives. The traditional mode of teaching was adopted among 63 medical students in the control group. Students took the after-class test and the scores were compared using normal distribution detection and independent sample t-test. Re s ults At the end of the course, the scores of the experimental group and the control group were all in line with the normal distribution P = 0.641 and P = 0.098 (> 0.05) , and the average score of the experimental group was significantly higher than that of the control group P = 0.00 (<0.01) . Conclus ion The taxonomy of Bloom's Taxonomy of Educational Objectives should be used in the teaching of the theory course of obstetrics and gynecology, which can improve the quality of teaching.
