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RSS2018 Vol. 39, No. 05
2018, 39(05): 1-6.
Abstract:
Objective To research the monoclonal antibody KMP1 inhibited bladder cancer EJ cell lines growth and metastasis in vivo by bioluminescence imaging. Me thods Immunohistochemistry was used to determine the KMP1 binding to EJ and EJ-GFP cell lines. The xenograft tumor cell growth and distribution were measured by vernier calipers and dynamic in vivo fluorescence imaging. Immunohistochemistry and H&E counterstaining researched the feature of the xenograft tumor. Re s ults Cell growth curves of EJ and EJ-GFP cells were similar.EJ-GFP had a green fluorescence. In EJ-GFP nude mouse tumor model, the addition of KMP1 significantly inhibited tumor growth and extended the average life span of nude mice. Both EJ and EJ-GFP cells can bind to KMP1, and the weight of transplanted tumors in the KMP1 treatment group was significantly lower than that of the m Ig G control group (P <0.001) .Conclus ion KMP1 has a promising antitumor effect in vivo. It might be valuable for development as a promising targeted agent for bladder cancer.
Objective To research the monoclonal antibody KMP1 inhibited bladder cancer EJ cell lines growth and metastasis in vivo by bioluminescence imaging. Me thods Immunohistochemistry was used to determine the KMP1 binding to EJ and EJ-GFP cell lines. The xenograft tumor cell growth and distribution were measured by vernier calipers and dynamic in vivo fluorescence imaging. Immunohistochemistry and H&E counterstaining researched the feature of the xenograft tumor. Re s ults Cell growth curves of EJ and EJ-GFP cells were similar.EJ-GFP had a green fluorescence. In EJ-GFP nude mouse tumor model, the addition of KMP1 significantly inhibited tumor growth and extended the average life span of nude mice. Both EJ and EJ-GFP cells can bind to KMP1, and the weight of transplanted tumors in the KMP1 treatment group was significantly lower than that of the m Ig G control group (P <0.001) .Conclus ion KMP1 has a promising antitumor effect in vivo. It might be valuable for development as a promising targeted agent for bladder cancer.
2018, 39(05): 7-10.
Abstract:
Objective To investigate the gene mutations of calcium-and integrin-binding protein 2 (CIB2) 196 C>T, 272 T > C and 297 C > G carried by students with non-syndromic hearing impairment from special educational schools in Yunnan Province. Me thods The experimental group included 337 students with non-syndromic hearing impairment who failed to carry deafness gene with GJB2 (35 del G, 176＿191 del16, 235 del C, 299＿300 del AT) , GJB3 (C538 T, G547 A) , mt DNA 12 S r RNA (A1555 G, C1494 T) , and SLC26 A4 (IVS7＿2 A>G, A2168 G) . The control group consisted with 150 healthy people. Genomic DNA was isolated from peripheral blood with EDTA anti-coagulate. The subject's DNA fragments including CIB2 196 C>T, 272 T > C and 297 C > G were amplified by polymerase chain reaction (PCR) , and subsequently analyzed by direct sequencing to identify deafness-associated mutations. Re s ults Both in the experimental group and control group, we failed to find the mutation of CIB2 196 C>T, 272 T > C and 297 C > G in all individuals. Conclus ion Mutations in CIB2 gene 196 C>T, 272 T > C and 297 C > G are not a frequent cause of non-syndromic hearing loss among deaf people in Yunnan province. It provided important information for deafness with formulating landscape of gene screening in this region.
Objective To investigate the gene mutations of calcium-and integrin-binding protein 2 (CIB2) 196 C>T, 272 T > C and 297 C > G carried by students with non-syndromic hearing impairment from special educational schools in Yunnan Province. Me thods The experimental group included 337 students with non-syndromic hearing impairment who failed to carry deafness gene with GJB2 (35 del G, 176＿191 del16, 235 del C, 299＿300 del AT) , GJB3 (C538 T, G547 A) , mt DNA 12 S r RNA (A1555 G, C1494 T) , and SLC26 A4 (IVS7＿2 A>G, A2168 G) . The control group consisted with 150 healthy people. Genomic DNA was isolated from peripheral blood with EDTA anti-coagulate. The subject's DNA fragments including CIB2 196 C>T, 272 T > C and 297 C > G were amplified by polymerase chain reaction (PCR) , and subsequently analyzed by direct sequencing to identify deafness-associated mutations. Re s ults Both in the experimental group and control group, we failed to find the mutation of CIB2 196 C>T, 272 T > C and 297 C > G in all individuals. Conclus ion Mutations in CIB2 gene 196 C>T, 272 T > C and 297 C > G are not a frequent cause of non-syndromic hearing loss among deaf people in Yunnan province. It provided important information for deafness with formulating landscape of gene screening in this region.
2018, 39(05): 11-15.
Abstract:
Objective To research the derivatives of panax notoginseng sapogenins and their anti-tumor activities. Me thods The ginsenosides Rg1 was treated with Smith degradation. The products were separated and purified by silica gel column chromatography. The structures of the products were determined by NMR spectra. The activity of anti-tumor cells of compound (1) and 1'-hydroxyethanedioxy PT was detected with CDC25 B activity assay and fluorescence technique. Re s ults 1'-hydroxyethanedioxy PT, 20 (s) -protopanaxatriol (PT) , and24, 25-en-3β, 6 α-dihydroxy-12, 20- (1', 2'-isopropylidenedioxy) propanedioxy-dammarane (1) were isolated and identified. Conclus ion Compound (1) , named 1', 2'-isopropylidenedioxy-propanedioxy-propanedioxy, is a novel derivative of panax notoginseng sapogenin with better inhibitory activity against CDC25 B.
Objective To research the derivatives of panax notoginseng sapogenins and their anti-tumor activities. Me thods The ginsenosides Rg1 was treated with Smith degradation. The products were separated and purified by silica gel column chromatography. The structures of the products were determined by NMR spectra. The activity of anti-tumor cells of compound (1) and 1'-hydroxyethanedioxy PT was detected with CDC25 B activity assay and fluorescence technique. Re s ults 1'-hydroxyethanedioxy PT, 20 (s) -protopanaxatriol (PT) , and24, 25-en-3β, 6 α-dihydroxy-12, 20- (1', 2'-isopropylidenedioxy) propanedioxy-dammarane (1) were isolated and identified. Conclus ion Compound (1) , named 1', 2'-isopropylidenedioxy-propanedioxy-propanedioxy, is a novel derivative of panax notoginseng sapogenin with better inhibitory activity against CDC25 B.
2018, 39(05): 16-20.
Abstract:
Objective To establish an acute yunaconitine poisoning rat model with a single oral administration and to determine the contents of yunaconitine in rat tissues by UPLC-MS/MS method, then investigate the distribution of yunaconitine in rats. Me thod The rats were randomly divided into three groups and were intragastrically administered a single dose of 2.2 mg/kg, 1.1 mg/kg, 0.7 mg/kg yunaconitine, respectively.. The rats were killed 2 h later, the stomach tissue, intestine tissue, liver tissue, pancreas tissue, kidney tissue, lung tissue, spleen tissue, heart tissue, bladder tissue, testis tissue, brain tissue and heart blood samples were collected. The contents of yunaconitine in the biological materials were determined by UPLC-MS/MS method after the biological samples extracted by liquid-liquid extraction. Re s ult A rat model of the yunaconitine poisoning was made with a single dose of 1.1 mg/kg, the concentrations of yunaconitine displayed in the organs with the following order:stomach, small intestine, liver, pancreas, kidney, lung, spleen, heart, bladder, testis, heart blood and brain. Conclus ion Yunaconitine was widely distributed in rats, especially the levels in the stomach, small intestine and liver were the highest. The conclusion provides a basis for the selection of test materials for the poisoning of Aconitum vilmorinianum Kom.
Objective To establish an acute yunaconitine poisoning rat model with a single oral administration and to determine the contents of yunaconitine in rat tissues by UPLC-MS/MS method, then investigate the distribution of yunaconitine in rats. Me thod The rats were randomly divided into three groups and were intragastrically administered a single dose of 2.2 mg/kg, 1.1 mg/kg, 0.7 mg/kg yunaconitine, respectively.. The rats were killed 2 h later, the stomach tissue, intestine tissue, liver tissue, pancreas tissue, kidney tissue, lung tissue, spleen tissue, heart tissue, bladder tissue, testis tissue, brain tissue and heart blood samples were collected. The contents of yunaconitine in the biological materials were determined by UPLC-MS/MS method after the biological samples extracted by liquid-liquid extraction. Re s ult A rat model of the yunaconitine poisoning was made with a single dose of 1.1 mg/kg, the concentrations of yunaconitine displayed in the organs with the following order:stomach, small intestine, liver, pancreas, kidney, lung, spleen, heart, bladder, testis, heart blood and brain. Conclus ion Yunaconitine was widely distributed in rats, especially the levels in the stomach, small intestine and liver were the highest. The conclusion provides a basis for the selection of test materials for the poisoning of Aconitum vilmorinianum Kom.
2018, 39(05): 21-28.
Abstract:
Objective To explore the influence of cyasterone on the osteoclast and osteoblast differentiation and then to investigate its effect on the bone quality in the osteoporosis mice. Me thods CCK8 assay was firstly used to detect the toxic effect of cyasterone on the mouse bone marrow derived mononuclear macrophages (BMMs) and anterior osteoblast lines MC3 T3 E1. Cell apoptosis was measured by flow cytometry. Then TRAP staining and ALP staining were employed to detect osteoclast differentiation and osteoblast differentiation, respectively. Realtime PCR was carried out to test the expression of osteoclast special gene TRAP and osteogenesis crucial gene ALP. In vivo, 15 mice were divided into three groups: sham-operated group, OVX group and OVX + cyasterone treatment group. In treatment group, cyasterone was used as 5 mg/kg every day. Sham-operated group and OVX group were treat with saline solution. After 4 weeks, the tibia was collected for Micro-CT detection to observe the bone quality and microstructure changes. Re s ults Cyasterone with the concentration of less than 10 mg/L had no significant cytotoxicity nor influence on the apoptosis (P >0.05) . Cyasterone could significantly inhibit the osteoclast differentiation of BMMs (P<0.05) , simultaneously, it also had the effect to promote the osteoblast differetiation of MC3 T3 E1. Real-time PCR indicated that cyasterone could block the expression of TRAP and increase the expression of ALP (P<0.05) . In vivo, cyasterone was able to obviously improve the osteoporosis status caused by estrogen deficiency without general toxicity. Conclus ion cyasterone could provide a good treatment for osteoporosis through the bidirectional effect of inhibiting osteoclast differetiation and promoting osteoblast differentiation.
Objective To explore the influence of cyasterone on the osteoclast and osteoblast differentiation and then to investigate its effect on the bone quality in the osteoporosis mice. Me thods CCK8 assay was firstly used to detect the toxic effect of cyasterone on the mouse bone marrow derived mononuclear macrophages (BMMs) and anterior osteoblast lines MC3 T3 E1. Cell apoptosis was measured by flow cytometry. Then TRAP staining and ALP staining were employed to detect osteoclast differentiation and osteoblast differentiation, respectively. Realtime PCR was carried out to test the expression of osteoclast special gene TRAP and osteogenesis crucial gene ALP. In vivo, 15 mice were divided into three groups: sham-operated group, OVX group and OVX + cyasterone treatment group. In treatment group, cyasterone was used as 5 mg/kg every day. Sham-operated group and OVX group were treat with saline solution. After 4 weeks, the tibia was collected for Micro-CT detection to observe the bone quality and microstructure changes. Re s ults Cyasterone with the concentration of less than 10 mg/L had no significant cytotoxicity nor influence on the apoptosis (P >0.05) . Cyasterone could significantly inhibit the osteoclast differentiation of BMMs (P<0.05) , simultaneously, it also had the effect to promote the osteoblast differetiation of MC3 T3 E1. Real-time PCR indicated that cyasterone could block the expression of TRAP and increase the expression of ALP (P<0.05) . In vivo, cyasterone was able to obviously improve the osteoporosis status caused by estrogen deficiency without general toxicity. Conclus ion cyasterone could provide a good treatment for osteoporosis through the bidirectional effect of inhibiting osteoclast differetiation and promoting osteoblast differentiation.
2018, 39(05): 29-34.
Abstract:
Objective To explore the effects of PPARγ agonist rosiglitazone and inhibitor T0070907 on apoptosis and anti-tumor in renal carcinoma A498 cells.Me thods A498 cells were divided into three groups and PBS, rosiglitazone (50 μmol/L) and T0070907 50 (μmol/L) were added respectively of 24 h incubation completely. each group of cell proliferation was determined by MTT method and Western Blot analysis and RT-q PCR were applied to detect the expression level of BAX, Caspase 3, Cyt C and Bcl-2. A498 cell morphological changes were observed under light microscope and fluorescence microscope. Re s ults MTT experiment results showed that rosiglitazone and T0070907 could significantly inhibit A498 cell proliferation rate (P< 0.05) , increased the protein and m RNA expression levels of Caspase 3, Cyt C and Bax in A498 cell, and decreased the protein and m RNA expression levels of Bcl-2 (P <0.05) ; Microscopic observation and Hochest staining also found that rosiglitazone and T0070907 could promote apoptosis of A498 cells. Conclus ion Rosiglitazone and T0070907 can inhibit the proliferation of renal cell carcinoma A498 cells and induce apoptosis. The anti-tumor mechanism may be related to PPARγ mediation.
Objective To explore the effects of PPARγ agonist rosiglitazone and inhibitor T0070907 on apoptosis and anti-tumor in renal carcinoma A498 cells.Me thods A498 cells were divided into three groups and PBS, rosiglitazone (50 μmol/L) and T0070907 50 (μmol/L) were added respectively of 24 h incubation completely. each group of cell proliferation was determined by MTT method and Western Blot analysis and RT-q PCR were applied to detect the expression level of BAX, Caspase 3, Cyt C and Bcl-2. A498 cell morphological changes were observed under light microscope and fluorescence microscope. Re s ults MTT experiment results showed that rosiglitazone and T0070907 could significantly inhibit A498 cell proliferation rate (P< 0.05) , increased the protein and m RNA expression levels of Caspase 3, Cyt C and Bax in A498 cell, and decreased the protein and m RNA expression levels of Bcl-2 (P <0.05) ; Microscopic observation and Hochest staining also found that rosiglitazone and T0070907 could promote apoptosis of A498 cells. Conclus ion Rosiglitazone and T0070907 can inhibit the proliferation of renal cell carcinoma A498 cells and induce apoptosis. The anti-tumor mechanism may be related to PPARγ mediation.
2018, 39(05): 35-38.
Abstract:
Objective to understand the current situation of the social adaptation ability of Kunming Medical University graduates and to explore the possible influence factors, so as to provide some references for the school to formulate effective countermeasures to improve college students' social adaptability.Me thods The method of census was adopted in 1 228 graduates of Kunming Medical University with a self-administered questionnaire.Re s ults The social adaptation ability of graduates is poor, accounting for 63.43%, while for students' financial situation, parents educational level, the number of joining college clubs, the quantity of the awards and qualification certificates, awareness of employment policy, the time to focusing on employment, and job prospects were statistically significant with social adaptation ability (P <0.05) .Conclus ion The social adaptability of the graduates in our school is poor, and the reasonable countermeasures should be formulated in view of the key factors.
Objective to understand the current situation of the social adaptation ability of Kunming Medical University graduates and to explore the possible influence factors, so as to provide some references for the school to formulate effective countermeasures to improve college students' social adaptability.Me thods The method of census was adopted in 1 228 graduates of Kunming Medical University with a self-administered questionnaire.Re s ults The social adaptation ability of graduates is poor, accounting for 63.43%, while for students' financial situation, parents educational level, the number of joining college clubs, the quantity of the awards and qualification certificates, awareness of employment policy, the time to focusing on employment, and job prospects were statistically significant with social adaptation ability (P <0.05) .Conclus ion The social adaptability of the graduates in our school is poor, and the reasonable countermeasures should be formulated in view of the key factors.
2018, 39(05): 39-44.
Abstract:
Objective To improve the early diagnosis of congenital heart disease (CHD) , investigate the awareness, screening knowledge and screening ability of CHD among health care providers in the rural areas of Yunnan province and discover an effective way to improve the related knowledge and skills of CHD screening.Me thods From 2015 September to 2017 April, we set up charity mobile schools and chose eight areas as the investigating and training sites. We designed two questionnaires, and adopted the theoretical training as the main method combined with the training of clinical skills.We investigated and trained a total of 1022 medical staff.The training content include the awareness of CHD, the knowledge and skills of CHD screening.We also evaluated the outcomes of the training.Re s ultsA total of 2044 questionnaires were collected.After training, the number of objects which would screen CHD in future increased, and the cognition of the incidence and common clinical manifestations of CHD, Eisenmanger's syndrome, cardiac auscultation, pulse oximetry and the related knowledge of CHD were also improved;563 took the test on practical skills of CHD detection.The correct rate of auscultation, period of murmurs and characteristics of murmurs was 98.22% (553) , 30.55% (172) and 28.60% (161) .The correct rate of murmurs in ventricular septal defect, atrial septal defect and patent ductus arteriosus was 45.47% (256) , 39.79 (224) and 50.80% (286) respectively.Online training was advised by 244 trainees.Increasing investment in congenital heart disease screening was suggested by 652 trainees.Conclus ionsThe awareness of CHD screening in grassroots medical workers of Yunnan province is insufficient.It is an effective way to establish charity mobile school and to train the grassroots medical staff with the theory and practical knowledge to improve the awareness, screening knowledge and screening skills of CHD.The next step suggests that we can improve the early diagnosis of congenital heart disease (CHD) .
Objective To improve the early diagnosis of congenital heart disease (CHD) , investigate the awareness, screening knowledge and screening ability of CHD among health care providers in the rural areas of Yunnan province and discover an effective way to improve the related knowledge and skills of CHD screening.Me thods From 2015 September to 2017 April, we set up charity mobile schools and chose eight areas as the investigating and training sites. We designed two questionnaires, and adopted the theoretical training as the main method combined with the training of clinical skills.We investigated and trained a total of 1022 medical staff.The training content include the awareness of CHD, the knowledge and skills of CHD screening.We also evaluated the outcomes of the training.Re s ultsA total of 2044 questionnaires were collected.After training, the number of objects which would screen CHD in future increased, and the cognition of the incidence and common clinical manifestations of CHD, Eisenmanger's syndrome, cardiac auscultation, pulse oximetry and the related knowledge of CHD were also improved;563 took the test on practical skills of CHD detection.The correct rate of auscultation, period of murmurs and characteristics of murmurs was 98.22% (553) , 30.55% (172) and 28.60% (161) .The correct rate of murmurs in ventricular septal defect, atrial septal defect and patent ductus arteriosus was 45.47% (256) , 39.79 (224) and 50.80% (286) respectively.Online training was advised by 244 trainees.Increasing investment in congenital heart disease screening was suggested by 652 trainees.Conclus ionsThe awareness of CHD screening in grassroots medical workers of Yunnan province is insufficient.It is an effective way to establish charity mobile school and to train the grassroots medical staff with the theory and practical knowledge to improve the awareness, screening knowledge and screening skills of CHD.The next step suggests that we can improve the early diagnosis of congenital heart disease (CHD) .
2018, 39(05): 45-49.
Abstract:
Objective To explore the expression of miR-223-3 p in the plasma of patients with diabetic kidney disease and its clinical significance. Me thods The expression levels of plasma miR-223-3 p in normoalbuminuric group (DM) , microoalbuminuria group (Micro-DKD) , macroalbuminuria group (Macro-DKD) and healthy controls were measured by quantitative real-time polymerase chain reaction (q RT-PCR) . To analysis the relationship with clinical pathological parameters, target genes of miR-223-3 p were predicted with bioinformatics software. Re s ults The levels of miR-223-3 p in the plasma of the remaining three groups were significantly lower than those in the healthy controls (P <0.001) , and the decrease was positively correlated with the severity of the disease. The potential target genes of miR-223-3 p identified by bioinformatics softwares include IL6 ST and PRKCE. Conclus ion The expressionof miR-223-3 pin diabetic kidney disease (DKD) patients' plasma decreased and was positively correlated with the severity of the disease. It may play an important role in the development and progression of diabetic kidney disease through its target genes.
Objective To explore the expression of miR-223-3 p in the plasma of patients with diabetic kidney disease and its clinical significance. Me thods The expression levels of plasma miR-223-3 p in normoalbuminuric group (DM) , microoalbuminuria group (Micro-DKD) , macroalbuminuria group (Macro-DKD) and healthy controls were measured by quantitative real-time polymerase chain reaction (q RT-PCR) . To analysis the relationship with clinical pathological parameters, target genes of miR-223-3 p were predicted with bioinformatics software. Re s ults The levels of miR-223-3 p in the plasma of the remaining three groups were significantly lower than those in the healthy controls (P <0.001) , and the decrease was positively correlated with the severity of the disease. The potential target genes of miR-223-3 p identified by bioinformatics softwares include IL6 ST and PRKCE. Conclus ion The expressionof miR-223-3 pin diabetic kidney disease (DKD) patients' plasma decreased and was positively correlated with the severity of the disease. It may play an important role in the development and progression of diabetic kidney disease through its target genes.
2018, 39(05): 50-55.
Abstract:
Objective Using the function of 256-layer i CT in the determination of cardiac function and arterial elasticity index, to compare the arterial elasticity, left ventricular function and their correlation between patients with aortic dissection and non-dissected controls, and to provide a reference for the clinical treatment of patients with aortic dissection. Me thods This study selected 36 cases in our emergency department, with chest, back or abdominal pain, whose clinical symptoms were highly suggestive of aortic dissection, and plain or enhanced diagnosis confirmed aortic dissection as a case group. At the same time, we randomly selected 40 cases without aortic dissection as the control group. All subjects underwent CTA one-stop scanning and measurement of elastic and cardiac function at the same level. According to the measurement results, the arterial elasticity indexes and the measured cardiac function of the case group and the control group were evaluated and compared so that to evaluate the arterial elasticity and its correlation with left ventricular function in patients with aortic dissection.Re s ults patients' s arterial expansion and compliance in patients with aortic dissection were significantly lower than the control group (P <0.05) . The mean diameter change rate and stiffness of the patients with dissection were significantly lower than those in the control group (P>0.05) . The difference was not statistically significant. The mean stiffness in patients with aortic dissection was significantly higher than non-aortic dissection patients. In patients with aortic dissection, the mean arterial diameter change rate, expandability and arterial stiffness showed a highly negative correlation. There was no significant correlation between arterial elasticity index and cardiac function in patients with Stanford type A and Stanford type B aortic dissection. There was no significant correlation between each elasticity index and cardiac function in the control group (r <0.3) . Conclus ion CTA one-stop scan can be used as an important measure of arterial elasticity, cardiac function and other parameters in patients with aortic dissection. Significantly lower aortic expansion and compliance, increased stiffness, insignificant changes in cardiac function, and insignificant correlation between cardiac function and each elasticity index in patients with early aortic dissection all have a clinical significance.
Objective Using the function of 256-layer i CT in the determination of cardiac function and arterial elasticity index, to compare the arterial elasticity, left ventricular function and their correlation between patients with aortic dissection and non-dissected controls, and to provide a reference for the clinical treatment of patients with aortic dissection. Me thods This study selected 36 cases in our emergency department, with chest, back or abdominal pain, whose clinical symptoms were highly suggestive of aortic dissection, and plain or enhanced diagnosis confirmed aortic dissection as a case group. At the same time, we randomly selected 40 cases without aortic dissection as the control group. All subjects underwent CTA one-stop scanning and measurement of elastic and cardiac function at the same level. According to the measurement results, the arterial elasticity indexes and the measured cardiac function of the case group and the control group were evaluated and compared so that to evaluate the arterial elasticity and its correlation with left ventricular function in patients with aortic dissection.Re s ults patients' s arterial expansion and compliance in patients with aortic dissection were significantly lower than the control group (P <0.05) . The mean diameter change rate and stiffness of the patients with dissection were significantly lower than those in the control group (P>0.05) . The difference was not statistically significant. The mean stiffness in patients with aortic dissection was significantly higher than non-aortic dissection patients. In patients with aortic dissection, the mean arterial diameter change rate, expandability and arterial stiffness showed a highly negative correlation. There was no significant correlation between arterial elasticity index and cardiac function in patients with Stanford type A and Stanford type B aortic dissection. There was no significant correlation between each elasticity index and cardiac function in the control group (r <0.3) . Conclus ion CTA one-stop scan can be used as an important measure of arterial elasticity, cardiac function and other parameters in patients with aortic dissection. Significantly lower aortic expansion and compliance, increased stiffness, insignificant changes in cardiac function, and insignificant correlation between cardiac function and each elasticity index in patients with early aortic dissection all have a clinical significance.
2018, 39(05): 56-60.
Abstract:
Objective To evaluate the effects of different dosages of rocuronium on abdominal wound closure under general anesthesia.Me thods Ninety ASA class I or II patients scheduled for elective abdominal surgery under general anesthesia were randomized into three groups with 30 cases each.Each group received rocuronium 1 time of ED95 (0.3 mg/kg) , 0.5 time of ED95 (0.15 mg/kg) , 0.2 time of ED95 (0.06 mg/kg) respectively when closing the abdominal wall.TOF recovered to 25%, 75%, 90%, time of eye-opening on command, time of head-lift for more than 5 seconds, extubation time, the rate of upper airways obstruction after extubation and the satisfaction score for the muscle relaxation to general surgeons were recorded.Re s ults TOF values had no significantly different among the three groups before abdominal wound closure (P>0.05) .Compared with group A and group B, time of TOF in group C was significangtly increased after administration of rocuronium (P<0.05) .Time of TOF recovery to 25%, 75%, 90% in group C were significangtly decreased (P<0.01) .Compared with group A and group B, time of eye-opening on command, head-lift for more than 5 seconds and extubation time were significantly shorter in group C.There were 8, 4, 0 patients undwent upper airways obstruction among each group respectively after extubation (P<0.05) .The satisfaction score for muscle relaxation among three groups had no significant difference.Conclus ion An additional administration of 0.2 time of ED95 (0.06 mg/kg) rocuronium before abdominal wound closure in abdominal surgery can achieve satisfactory muscle relaxation for surgeons.
Objective To evaluate the effects of different dosages of rocuronium on abdominal wound closure under general anesthesia.Me thods Ninety ASA class I or II patients scheduled for elective abdominal surgery under general anesthesia were randomized into three groups with 30 cases each.Each group received rocuronium 1 time of ED95 (0.3 mg/kg) , 0.5 time of ED95 (0.15 mg/kg) , 0.2 time of ED95 (0.06 mg/kg) respectively when closing the abdominal wall.TOF recovered to 25%, 75%, 90%, time of eye-opening on command, time of head-lift for more than 5 seconds, extubation time, the rate of upper airways obstruction after extubation and the satisfaction score for the muscle relaxation to general surgeons were recorded.Re s ults TOF values had no significantly different among the three groups before abdominal wound closure (P>0.05) .Compared with group A and group B, time of TOF in group C was significangtly increased after administration of rocuronium (P<0.05) .Time of TOF recovery to 25%, 75%, 90% in group C were significangtly decreased (P<0.01) .Compared with group A and group B, time of eye-opening on command, head-lift for more than 5 seconds and extubation time were significantly shorter in group C.There were 8, 4, 0 patients undwent upper airways obstruction among each group respectively after extubation (P<0.05) .The satisfaction score for muscle relaxation among three groups had no significant difference.Conclus ion An additional administration of 0.2 time of ED95 (0.06 mg/kg) rocuronium before abdominal wound closure in abdominal surgery can achieve satisfactory muscle relaxation for surgeons.
2018, 39(05): 61-65.
Abstract:
Objective To investigate epidemiological characteristics of pathogenic bacteria of community-acquired lower respiratory tract infection in children in Kunming (with an altitude of 1860 m) .Me thods The results of bacterial culture of 9793 patients with community-acquired lower respiratory tract infection in the Second Affiliated Hospital of Kunming Medical University and Kunming Maternal and Child Health Hospital from Jan 2011 to Nov 2015 were reviewed.Re s ults Among the 9793 phlegm samples from the children patients with community-acquired lower respiratory tract infection, 8433 (86.10%) phlegm samples were qualified, 2918 (34.6%) had a positive bacterial culture.The pathogenic bacteria mainly were gram-negative bacteria (1 839 strains, 58.9%) , and gram-positive bacteria (1 284 strains, 41.4%) . The detection rate of gram-negative bacteria was increasing, among which Haemophilus was in a leading position (768 strains, 24.60%) , followed by Streptococcus pneumonia (665 strains, 21.3%) , E. coli (315 strains, 10.10%) , Staphylococcus aureus (310 strains, 9.9%) , Moraxella catarrhalis (272 strains, 8.7%) , and Klebsiella pneumoniae bacillus (224 strains, 7.2%) . The detection rates of six common bacteria in different seasons were significantly different (P<0.05) . The detection rates of six common bacteria except Moraxella catarrhalis in different age groups were significantly different (P<0.05) . Conclus ions Gram-negative bacterium was the main pathogenic bacteria for those children who got community-acquired lower respiratory tract infections. Haemophilus influenza was the most common pathogenic bacteria and the detection rate of pathogenic bacteria in winter was high. The most common pathogenic bacteria in infants and toddler were Haemophilus influenza. The most common pathogen in preschool and school-age children was Streptococcus pneumonia.The pathogenic bacteria of lower respiratory tract infection in the children community of Kunming are different from those in the plain area.
Objective To investigate epidemiological characteristics of pathogenic bacteria of community-acquired lower respiratory tract infection in children in Kunming (with an altitude of 1860 m) .Me thods The results of bacterial culture of 9793 patients with community-acquired lower respiratory tract infection in the Second Affiliated Hospital of Kunming Medical University and Kunming Maternal and Child Health Hospital from Jan 2011 to Nov 2015 were reviewed.Re s ults Among the 9793 phlegm samples from the children patients with community-acquired lower respiratory tract infection, 8433 (86.10%) phlegm samples were qualified, 2918 (34.6%) had a positive bacterial culture.The pathogenic bacteria mainly were gram-negative bacteria (1 839 strains, 58.9%) , and gram-positive bacteria (1 284 strains, 41.4%) . The detection rate of gram-negative bacteria was increasing, among which Haemophilus was in a leading position (768 strains, 24.60%) , followed by Streptococcus pneumonia (665 strains, 21.3%) , E. coli (315 strains, 10.10%) , Staphylococcus aureus (310 strains, 9.9%) , Moraxella catarrhalis (272 strains, 8.7%) , and Klebsiella pneumoniae bacillus (224 strains, 7.2%) . The detection rates of six common bacteria in different seasons were significantly different (P<0.05) . The detection rates of six common bacteria except Moraxella catarrhalis in different age groups were significantly different (P<0.05) . Conclus ions Gram-negative bacterium was the main pathogenic bacteria for those children who got community-acquired lower respiratory tract infections. Haemophilus influenza was the most common pathogenic bacteria and the detection rate of pathogenic bacteria in winter was high. The most common pathogenic bacteria in infants and toddler were Haemophilus influenza. The most common pathogen in preschool and school-age children was Streptococcus pneumonia.The pathogenic bacteria of lower respiratory tract infection in the children community of Kunming are different from those in the plain area.
2018, 39(05): 66-69.
Abstract:
Objective To investigate the pharmacokinetics and pharmacokinetics of torsemide in children with acute heart failure.Me thods Ninety cases of children with acute heart failure patients were randomly divided into three groups which were given different intravenous therapy doses of torsemide. Our goal is to provide the basis for clinical rational therapy by analyzing parameter of the pharmacokinetics and pharmacokinetics of torsemide which is acquired by detecting plasma concentration of torsemide with liquid chromatography and tandem mass spectrometry after a one-time medication. Re s ult Average pharmacokinetic parameters of the three groups in addition to the peak concentration (Cmax) and medication in the area under the curve (AUC0-16) are different (P < 0.01) , the rest of the pharmacokinetic parameters had no significant difference (P > 0.05) . The 24 hours urine volume of the experimental group Ⅱ and the experimental group Ⅲ were obviously higher than that of the experimental group Ⅰ, so the difference was statistically significant (P < 0.01) .The 24 hours urine volumes between the experimental group Ⅱ and the experimental group Ⅲ were no significant difference (P > 0.05) .There was no significant change in blood pressure, weight, abdominal girth, blood potassium, blood sodium and blood chlorine in the three dose groups compared with those before the treatment.There was no significant difference between the three groups (P>0.05) .Conclus ionThe children with acute heart failure were well tolerated with torsemide.The recommended dose of torsemide is 1.0 mg/ (kg.d) in the treatment of acute heart failure in children, based on pharmacokinetic and pharmacodynamic characteristics.
Objective To investigate the pharmacokinetics and pharmacokinetics of torsemide in children with acute heart failure.Me thods Ninety cases of children with acute heart failure patients were randomly divided into three groups which were given different intravenous therapy doses of torsemide. Our goal is to provide the basis for clinical rational therapy by analyzing parameter of the pharmacokinetics and pharmacokinetics of torsemide which is acquired by detecting plasma concentration of torsemide with liquid chromatography and tandem mass spectrometry after a one-time medication. Re s ult Average pharmacokinetic parameters of the three groups in addition to the peak concentration (Cmax) and medication in the area under the curve (AUC0-16) are different (P < 0.01) , the rest of the pharmacokinetic parameters had no significant difference (P > 0.05) . The 24 hours urine volume of the experimental group Ⅱ and the experimental group Ⅲ were obviously higher than that of the experimental group Ⅰ, so the difference was statistically significant (P < 0.01) .The 24 hours urine volumes between the experimental group Ⅱ and the experimental group Ⅲ were no significant difference (P > 0.05) .There was no significant change in blood pressure, weight, abdominal girth, blood potassium, blood sodium and blood chlorine in the three dose groups compared with those before the treatment.There was no significant difference between the three groups (P>0.05) .Conclus ionThe children with acute heart failure were well tolerated with torsemide.The recommended dose of torsemide is 1.0 mg/ (kg.d) in the treatment of acute heart failure in children, based on pharmacokinetic and pharmacodynamic characteristics.
2018, 39(05): 70-77.
Abstract:
Objective To investigate the association between single nucleotide polymorphisms (SNPs) in CYP4A11 and Apo B、 Apo E genesandthe risk of essential hypertension. Me thods By means of correlation analysis, this case-controlled study included a total of 350 essential hypertension patients admitted in the first Hospital of Xi'an from June 2012 to December 2016, and another 350 cases with matched ages and sexes enrolled for routine check-ups as the healthy control group. The genotypes of CYP4A11 and Apo B、 Apo E genes were determined by Mass ARRAY method. SPSS 21.0 software was used to determine the correlation between SNPs and the risk of primary hypertension.Re s ults Comparing the allele frequencies of SNPs, we found rs1126742 (OR=1.45, 95 % CI, 1.09-1.92, P =0.008) 、 rs3890011 (OR=1.98, 95 % CI, 1.06-1.85, P =0.001) and rs9332978 (OR=1.54, 95 % CI, 1.27-1.91, P=0.004) on CYP4A11 were significantly associated with an increased risk of essential hypertension; Xba Ⅰ polymorphism in Apo B gene was associated with essential hypertension risks (OR=1.55, 95 % CI, 1.15-2.55, P=0.001) ;ε4 in Apo E gene was also found associated with essential hypertension risks (OR=1.49, 95 % CI, 1.09-2.35, P =0.012) , while ε2 andε3 not associated.Comparing the genotype frequencies of SNPs, we found the GG genotype of rs9332978, TC and CC genotype of rs1126742, CG and GG genotypes of rs3890011 were associated with increased risks of essential hypertension (P <0.05) .The X-X-genotype of Xba Ⅰ in Apo B gene were associated with increased risks of essential hypertension (OR=2.45, 95 % CI, 1.25-3.25, P = 0.035) . The E2/E4、E3/E4、E4/E4 genotype of Apo E were associated with increased risks of essential hypertension (P<0.05) .In the genetic model analysis, we found that the minor allele "A" of rs9332978 was associated with an increased risk of essential hypertension under dominant model (P <0.05) . The minor allele "T" of rs1126742 was associated with increased risks essential hypertension under dominant and recessive models (P<0.05) . Conclus ion Gene polymorphisms of CYP4A11、Apo B and Apo E play an important role in the occurrence and development of essential hypertension.
Objective To investigate the association between single nucleotide polymorphisms (SNPs) in CYP4A11 and Apo B、 Apo E genesandthe risk of essential hypertension. Me thods By means of correlation analysis, this case-controlled study included a total of 350 essential hypertension patients admitted in the first Hospital of Xi'an from June 2012 to December 2016, and another 350 cases with matched ages and sexes enrolled for routine check-ups as the healthy control group. The genotypes of CYP4A11 and Apo B、 Apo E genes were determined by Mass ARRAY method. SPSS 21.0 software was used to determine the correlation between SNPs and the risk of primary hypertension.Re s ults Comparing the allele frequencies of SNPs, we found rs1126742 (OR=1.45, 95 % CI, 1.09-1.92, P =0.008) 、 rs3890011 (OR=1.98, 95 % CI, 1.06-1.85, P =0.001) and rs9332978 (OR=1.54, 95 % CI, 1.27-1.91, P=0.004) on CYP4A11 were significantly associated with an increased risk of essential hypertension; Xba Ⅰ polymorphism in Apo B gene was associated with essential hypertension risks (OR=1.55, 95 % CI, 1.15-2.55, P=0.001) ;ε4 in Apo E gene was also found associated with essential hypertension risks (OR=1.49, 95 % CI, 1.09-2.35, P =0.012) , while ε2 andε3 not associated.Comparing the genotype frequencies of SNPs, we found the GG genotype of rs9332978, TC and CC genotype of rs1126742, CG and GG genotypes of rs3890011 were associated with increased risks of essential hypertension (P <0.05) .The X-X-genotype of Xba Ⅰ in Apo B gene were associated with increased risks of essential hypertension (OR=2.45, 95 % CI, 1.25-3.25, P = 0.035) . The E2/E4、E3/E4、E4/E4 genotype of Apo E were associated with increased risks of essential hypertension (P<0.05) .In the genetic model analysis, we found that the minor allele "A" of rs9332978 was associated with an increased risk of essential hypertension under dominant model (P <0.05) . The minor allele "T" of rs1126742 was associated with increased risks essential hypertension under dominant and recessive models (P<0.05) . Conclus ion Gene polymorphisms of CYP4A11、Apo B and Apo E play an important role in the occurrence and development of essential hypertension.
2018, 39(05): 78-82.
Abstract:
Objective To compare the short-term therapeutic effects of the caudal-to-crainal and medial-to-lateral approaches for laparoscopic right hemicolectomy. Me thod The clinical data of 124 patients underwent laparoscopic right hemicolectomy in the department of gastrointestinal surgery, the First Affiliated Hospital of Kunming Medical Universitiy from, June 2014 to June 2016, were analyzed retrospectively. According the surgical operation, the patients were divided into two groups, caudal-to-crainal group with 48 patients, and medial-to-lateral group with 76 patients. The characteristics, opertation time, volum of blood loss during operation, the number of lymph node dissection, the rate of conversion to laparotomy, postoperative eating time, postoperative ventilation time, postoperative hospital stay time, postoperative complications of the two groups were analyzed to compare the short-term therapeutic effects. Re s ult No significant differences were found in the sexual distinction, age, BMI, the volume blood loss during the operation, the number of lymph node dissection, the rate of conversion to laparotomy, postoperative eating time, postoperative ventilation time, postoperative hospital stay time, postoperative complications between the two groups (P>0.05) .Significant differences were found in the operation time [caudal-to-crainal group vs medial-to-lateral group (123.49 ±14.19 min VS 140.57 ±25.40 min) ]and the blood loss of the operation[caudal-to-crainal group vs medial-to-lateral group (60.63±24.00 ml vs77.24±36.90 ml) ].Conclus ionThe caudal-to-crainal approach for laparoscopic right hemicolectomy is more simple, practicable, with less blood loss during the operation and safer, which worth being recommended in right-hemicolectom-surgery.
Objective To compare the short-term therapeutic effects of the caudal-to-crainal and medial-to-lateral approaches for laparoscopic right hemicolectomy. Me thod The clinical data of 124 patients underwent laparoscopic right hemicolectomy in the department of gastrointestinal surgery, the First Affiliated Hospital of Kunming Medical Universitiy from, June 2014 to June 2016, were analyzed retrospectively. According the surgical operation, the patients were divided into two groups, caudal-to-crainal group with 48 patients, and medial-to-lateral group with 76 patients. The characteristics, opertation time, volum of blood loss during operation, the number of lymph node dissection, the rate of conversion to laparotomy, postoperative eating time, postoperative ventilation time, postoperative hospital stay time, postoperative complications of the two groups were analyzed to compare the short-term therapeutic effects. Re s ult No significant differences were found in the sexual distinction, age, BMI, the volume blood loss during the operation, the number of lymph node dissection, the rate of conversion to laparotomy, postoperative eating time, postoperative ventilation time, postoperative hospital stay time, postoperative complications between the two groups (P>0.05) .Significant differences were found in the operation time [caudal-to-crainal group vs medial-to-lateral group (123.49 ±14.19 min VS 140.57 ±25.40 min) ]and the blood loss of the operation[caudal-to-crainal group vs medial-to-lateral group (60.63±24.00 ml vs77.24±36.90 ml) ].Conclus ionThe caudal-to-crainal approach for laparoscopic right hemicolectomy is more simple, practicable, with less blood loss during the operation and safer, which worth being recommended in right-hemicolectom-surgery.
2018, 39(05): 83-89.
Abstract:
Objective To investigate the application of 3 D printing combined with S-ROM total hip arthroplasty in the treatment of adult Crowe IV DDH. Me thods Between September 2014 and June 2017, 10 patients of adult Crowe IV DDH were selected and divided into 3 D group (5 cases) and non-3 D group (5 cases) . There were 4 males and 6 females with an average age of 43.6 years (range, 27-63 years) . Harris scores were recorded in both groups before the operation, and in the group 3 D, patients underwent spiral CT scanning to make the hip 3 D models. According to the hip 3 D models, the sizes of prosthesis and operation schemeswere estimated before the operation, and the surgery simulation was performed. Then the S-ROM prosthesis was used for total hip arthroplasty. The actual situation of hip joint, the size of prosthesis, time of operation and blood loss were recorded.Harris Score at 1 week post-operationwere recorded again. All patients were followed up related postoperative complications for at least 2 weeks.Re s ults The 3 D printing models coordinated with the actual sizes of the hips in the3 D group.The time of operation and blood loss were less than the non-3 D group. Higher Harrisscore was observed at 1 week post-operation in the 3 D group, compared to non-3 D printing group.The follow-up showed that the motion of the hips was improved obviously. Conclus ion The Application of 3 D Printing combined with S-ROM total hip arthroplasty in the treatment of adult crowe IV DDH can improve efficiency and accuracy of the operation, shorten time of operation, reduce operative blood loss and rate of complications.
Objective To investigate the application of 3 D printing combined with S-ROM total hip arthroplasty in the treatment of adult Crowe IV DDH. Me thods Between September 2014 and June 2017, 10 patients of adult Crowe IV DDH were selected and divided into 3 D group (5 cases) and non-3 D group (5 cases) . There were 4 males and 6 females with an average age of 43.6 years (range, 27-63 years) . Harris scores were recorded in both groups before the operation, and in the group 3 D, patients underwent spiral CT scanning to make the hip 3 D models. According to the hip 3 D models, the sizes of prosthesis and operation schemeswere estimated before the operation, and the surgery simulation was performed. Then the S-ROM prosthesis was used for total hip arthroplasty. The actual situation of hip joint, the size of prosthesis, time of operation and blood loss were recorded.Harris Score at 1 week post-operationwere recorded again. All patients were followed up related postoperative complications for at least 2 weeks.Re s ults The 3 D printing models coordinated with the actual sizes of the hips in the3 D group.The time of operation and blood loss were less than the non-3 D group. Higher Harrisscore was observed at 1 week post-operation in the 3 D group, compared to non-3 D printing group.The follow-up showed that the motion of the hips was improved obviously. Conclus ion The Application of 3 D Printing combined with S-ROM total hip arthroplasty in the treatment of adult crowe IV DDH can improve efficiency and accuracy of the operation, shorten time of operation, reduce operative blood loss and rate of complications.
2018, 39(05): 90-93.
Abstract:
Objective To study the characteristics of colorectal cancer patients in Yunnan Tumor Hospital, and to provide the basis for the prevention and treatment of colorectal cancer. Me thods Retrospective analysis was used to review colorectal cancer patients who were diagnosed first and received the main treatment in Yunnan Cancer Hospital from March 2005 to December 2014.According to the sampling principle, there were 100 cases each year, with a total of 1000 cases. The clinical and pathological data were analyzed, including age, gender, pathogenesis, pathological type, and TNM stage. Re s ults The average age of the 1000 patients enrolled in the survey was (63.4 ± 12.8) years old, the male and female age group (60-69) accounted for the highest proportion.both men and women aged between 60 and 69 had a high occurance rate, and male patients were more than the female with a fraction of 1.42:1. Rectum is the most common primary site, accounting for 57%, followed by ascending colon, sigmoid colon, straight B junction, transverse colon, descending colon, and cecum.Adenocarcinoma was the main pathological type, accounting for 89.4%.Stage III was the most common in TNM staging, accounting for 35.9%, followed by stage II, I, and stage IV. Most rectal cancers were found at stage III, and colon cancer at stage II. Conclus ion The proportion of colorectal cancer in the age group (60-69 years) was the highest; the proportion of middle-aged and male was significant.The high incidence of colorectal cancer was60 ~ 69 years old, especially males.The main part of colorectal cancer was located in the rectum.Adenocarcinoma was the most common pathological type. Most patients were later stage when diagnosed.
Objective To study the characteristics of colorectal cancer patients in Yunnan Tumor Hospital, and to provide the basis for the prevention and treatment of colorectal cancer. Me thods Retrospective analysis was used to review colorectal cancer patients who were diagnosed first and received the main treatment in Yunnan Cancer Hospital from March 2005 to December 2014.According to the sampling principle, there were 100 cases each year, with a total of 1000 cases. The clinical and pathological data were analyzed, including age, gender, pathogenesis, pathological type, and TNM stage. Re s ults The average age of the 1000 patients enrolled in the survey was (63.4 ± 12.8) years old, the male and female age group (60-69) accounted for the highest proportion.both men and women aged between 60 and 69 had a high occurance rate, and male patients were more than the female with a fraction of 1.42:1. Rectum is the most common primary site, accounting for 57%, followed by ascending colon, sigmoid colon, straight B junction, transverse colon, descending colon, and cecum.Adenocarcinoma was the main pathological type, accounting for 89.4%.Stage III was the most common in TNM staging, accounting for 35.9%, followed by stage II, I, and stage IV. Most rectal cancers were found at stage III, and colon cancer at stage II. Conclus ion The proportion of colorectal cancer in the age group (60-69 years) was the highest; the proportion of middle-aged and male was significant.The high incidence of colorectal cancer was60 ~ 69 years old, especially males.The main part of colorectal cancer was located in the rectum.Adenocarcinoma was the most common pathological type. Most patients were later stage when diagnosed.
2018, 39(05): 94-99.
Abstract:
Objective To summarize the experience of partial tubeless mPCNL. Methods A retrospective analysis of partial tubeless mPCNL surgery experience from January 2010 to December 2016. Atotal of 1320 patients underwent mPCNL surgery in these 7 years.Out fo those 1320 patients, 554 patients underwent partial tubeless mPCNL, 766 patients underwent standard mPCNL, and 85 exception cases of standard mPCNL were forced to abort surgery due to maximum surgery time of 2 hours and different complications such as bleeding, infections and etc, so total of 681 patients with standard mPCNL were compared with partial tubeless mPCNL.Re s ults The rate of partialtubeless mPCNL has increased by 84% in 2016, with an indication of tubeless mPCNL being extended, while the complication rate showed no increase. Compared with the standard mPCNL, there was no significant difference between the two groups in the rate of stone removal, drop in mean hemoglobin, blood transfusion and postoperative fever. There were significant differences in postoperative analgesic use rate (5%:21%, P = 0.001) and hospitalization stay (2.5:4.5 d, P = 0.001) . The rate of postoperative bleeding complications in partial tubeless group and standard group is 1.1% and 2.5%respectively, but difference is not statistically significant. There were1 cases of urinary extravasations in the partial tubeless group which was treated by perirenal drainage, antibiotic treatment, and 1 cases of pleural injury, which were treated by open exploration, and chest tube placement.Conclus ion In compared to standard mPCNL partial tubeless mPCNL significantly increased patients postoperative comfort, shorten hospital stay, and no complications increased.Partial tubless mPCNL is a safe and practicableprocedure.
Objective To summarize the experience of partial tubeless mPCNL. Methods A retrospective analysis of partial tubeless mPCNL surgery experience from January 2010 to December 2016. Atotal of 1320 patients underwent mPCNL surgery in these 7 years.Out fo those 1320 patients, 554 patients underwent partial tubeless mPCNL, 766 patients underwent standard mPCNL, and 85 exception cases of standard mPCNL were forced to abort surgery due to maximum surgery time of 2 hours and different complications such as bleeding, infections and etc, so total of 681 patients with standard mPCNL were compared with partial tubeless mPCNL.Re s ults The rate of partialtubeless mPCNL has increased by 84% in 2016, with an indication of tubeless mPCNL being extended, while the complication rate showed no increase. Compared with the standard mPCNL, there was no significant difference between the two groups in the rate of stone removal, drop in mean hemoglobin, blood transfusion and postoperative fever. There were significant differences in postoperative analgesic use rate (5%:21%, P = 0.001) and hospitalization stay (2.5:4.5 d, P = 0.001) . The rate of postoperative bleeding complications in partial tubeless group and standard group is 1.1% and 2.5%respectively, but difference is not statistically significant. There were1 cases of urinary extravasations in the partial tubeless group which was treated by perirenal drainage, antibiotic treatment, and 1 cases of pleural injury, which were treated by open exploration, and chest tube placement.Conclus ion In compared to standard mPCNL partial tubeless mPCNL significantly increased patients postoperative comfort, shorten hospital stay, and no complications increased.Partial tubless mPCNL is a safe and practicableprocedure.
2018, 39(05): 100-105.
Abstract:
目的 分析急性下消化道大出血患者急诊肠镜诊断效果及影响因素.方法 选取凉山彝族自治州第一人民医院2016年5月至2017年5月收治的56例急性下消化道大出血患者, 均行急诊结肠镜检查, 分析急性下消化道大出血病因, 比较不同肠道准备方法、不同出血程度患者急诊肠镜检查完成情况, 依据急诊肠镜检查成功与否分为检查成功组、检查失败组, 比较2组临床资料, 分析影响急诊肠镜检查的影响因素.结果 急性下消化道大出血病因有结肠癌、结肠息肉、结肠憩室等;不同肠道准备方法诱发再出血或出血加重率无明显差异 (P>0.05) , 重度出血组活动性出血病灶发现率高于轻度出血组、中度出血组 (P<0.05)>45岁、阑尾切除史、服药期间步行时间短、肠道准备不充分、检查前未服完全部清肠药、肠道解剖结构异常、医师操作不规范比例低于检查失败组 (P<0.05)>45岁、肠道准备不充分、肠道解剖结构异常是急性下消化道大出血患者急诊肠镜检查失败的独立危险因素 (P<0.05)>45岁、肠道准备不充分、肠道解剖结构异常是影响其诊断效果的独立危险因素.
2018, 39(05): 106-110.
Abstract:
Objective Through the detection of MIP-1α and VEGF levels in serum and CSF of ALS patients, we evaluated the clinical value of MIP-1 and VEGF levels in ALS patients. Me thods A total of 80 patients with ALS were collected from Kuangwu Hospital of Tongchuanin from Jan, 2012 to Jun, 2015, and 67 patients with non-inflammation neurological diseases were chosen as control group. We obtained CSF and serum samples, and the MIP-1α and VEGF levels were measured by ELISA method. Re s ults The MIP-1α levels in serum and CSF of ALS patients were statistically and significantly higher than those in the control group (P<0.05) .The VEGF levels in serum of ALS patients were statistically and significantly higher than those in the control group (P<0.05) .The VEGF levels in CSF of ALS patients were not higher than those in the control group, statistically insignificant (P >0.05) .MIP-1 alpha and VEGF levels was positively correlated with ALS course. The MIP-1αand VEGF levels of the long duration group were greater than the short duration group (P <0.05) .Conclus ion The rising of MIP-1α and VEGF may indicate an activation of compensatory responses in ALS which suggested that MIP-1 alpha and VEGF are involved in the pathophysiology of amyotrophic lateral sclerosis. We propose that MIP-1α and VEGF may be a useful biomarker witha prognostic and evaluating potential for ALS.
Objective Through the detection of MIP-1α and VEGF levels in serum and CSF of ALS patients, we evaluated the clinical value of MIP-1 and VEGF levels in ALS patients. Me thods A total of 80 patients with ALS were collected from Kuangwu Hospital of Tongchuanin from Jan, 2012 to Jun, 2015, and 67 patients with non-inflammation neurological diseases were chosen as control group. We obtained CSF and serum samples, and the MIP-1α and VEGF levels were measured by ELISA method. Re s ults The MIP-1α levels in serum and CSF of ALS patients were statistically and significantly higher than those in the control group (P<0.05) .The VEGF levels in serum of ALS patients were statistically and significantly higher than those in the control group (P<0.05) .The VEGF levels in CSF of ALS patients were not higher than those in the control group, statistically insignificant (P >0.05) .MIP-1 alpha and VEGF levels was positively correlated with ALS course. The MIP-1αand VEGF levels of the long duration group were greater than the short duration group (P <0.05) .Conclus ion The rising of MIP-1α and VEGF may indicate an activation of compensatory responses in ALS which suggested that MIP-1 alpha and VEGF are involved in the pathophysiology of amyotrophic lateral sclerosis. We propose that MIP-1α and VEGF may be a useful biomarker witha prognostic and evaluating potential for ALS.
2018, 39(05): 111-114.
Abstract:
Objective To study and observe the effects of cold knife excision by hysteroscopy for the local microcirculation and inflammatory reaction of patients with hysteromyoma.Me thods 86 patients with hysteromyoma admitted in our hospital from March 2016 to July 2017 were selected as the research objects, and the patients were divided into control group with 43 cases and observation group with 43 cases according to the method of random number table.The control group were treated with monopolar electric knife excision by hysteroscopy, while the observation group treated with cold knife excision by hysteroscopy.Then the local microcirculation and inflammatory reaction indexes of two groups at different time before and after the operation were detected.Re s ults The local microcirculation and inflammatory reaction indexes of two groups before the operation were compare, no significant differences found (P>0.05) ;the local microcirculation indexes of observation group after the operation were significantly better than those of the control group, the inflammatory reaction indexes were significantly lower than those of the control group, both with statistical significance (P<0.05) .Conclus ion The cold knife excision by hysteroscopy has better clinical effects in the control of local microcirculation and inflammatory reaction in patients with hysteromyoma, so it has higher application value in the patients with hysteromyoma.
Objective To study and observe the effects of cold knife excision by hysteroscopy for the local microcirculation and inflammatory reaction of patients with hysteromyoma.Me thods 86 patients with hysteromyoma admitted in our hospital from March 2016 to July 2017 were selected as the research objects, and the patients were divided into control group with 43 cases and observation group with 43 cases according to the method of random number table.The control group were treated with monopolar electric knife excision by hysteroscopy, while the observation group treated with cold knife excision by hysteroscopy.Then the local microcirculation and inflammatory reaction indexes of two groups at different time before and after the operation were detected.Re s ults The local microcirculation and inflammatory reaction indexes of two groups before the operation were compare, no significant differences found (P>0.05) ;the local microcirculation indexes of observation group after the operation were significantly better than those of the control group, the inflammatory reaction indexes were significantly lower than those of the control group, both with statistical significance (P<0.05) .Conclus ion The cold knife excision by hysteroscopy has better clinical effects in the control of local microcirculation and inflammatory reaction in patients with hysteromyoma, so it has higher application value in the patients with hysteromyoma.
2018, 39(05): 115-119.
Abstract:
Objective To discover the treatment effect of arthroscope combined with proximal humerus osteotomy in Knee inversion osteoarthritis.Me thods From April 2012 to December 2016, 126 cases were diagnosed with knee inversion osteoarthritis in our deparment, then we divided them into two groups randomly, 63 in each group, the control group operated with arthroscope, and the observation group operated with arthroscope combined with proximal humerus osteotomy. The treatment effects of the two groups were compared. Re s ults Two groups of patients' HSS score, VAS score, KSS score before surgery have no statistical significance (P>0.05) . 6 months post-operation, the HSS score and KSS score have significant increase in both groups, while the VAS score has an obviously reduction. The differences between the two groups have statistical significance (P<0.05) , the treatment effects in two groups have statistical significance (P <0.05) , the observation group has better treatment effect than the control group, and in the case of adverse reactions in both groups, there were no statistical significance (P >0.05) . Conclus ion The treatment of middle-aged and elderly people knee inversion osteoarthritis by arthroscope combined with proximal humerus osteotomy could improve the treatment, soit is worth promoting in clinics.
Objective To discover the treatment effect of arthroscope combined with proximal humerus osteotomy in Knee inversion osteoarthritis.Me thods From April 2012 to December 2016, 126 cases were diagnosed with knee inversion osteoarthritis in our deparment, then we divided them into two groups randomly, 63 in each group, the control group operated with arthroscope, and the observation group operated with arthroscope combined with proximal humerus osteotomy. The treatment effects of the two groups were compared. Re s ults Two groups of patients' HSS score, VAS score, KSS score before surgery have no statistical significance (P>0.05) . 6 months post-operation, the HSS score and KSS score have significant increase in both groups, while the VAS score has an obviously reduction. The differences between the two groups have statistical significance (P<0.05) , the treatment effects in two groups have statistical significance (P <0.05) , the observation group has better treatment effect than the control group, and in the case of adverse reactions in both groups, there were no statistical significance (P >0.05) . Conclus ion The treatment of middle-aged and elderly people knee inversion osteoarthritis by arthroscope combined with proximal humerus osteotomy could improve the treatment, soit is worth promoting in clinics.
2018, 39(05): 120-123.
Abstract:
Objective To observe the efficacy and safety of DAAs in treating HCV patients with HIV/HCV co-infection.Me thod 53 patients were divided into groups based on HCV genotype. Sofosbuvir + Ledipasvir regime were used for 1 b and 6 a subtypes; sofosbuvir + Daclatasvir regime were used for 3 a, 3 b and those cannot be typed; all the patients diagnosed with cirrhosis were also administrated with ribavirin. The course of treatment for all patients is 12 weeks.Re s ults All 53 patients completed HCV treatment, the overall SVR rate of DAAs treatment rate was 98.1% (52/53) , failure rate 1.9% (1/53) ; SVR rate of DAAs treatment among non-cirrhosis patients was 100% (41/41) ; SVR rate of DAAs treatment among cirrhosis patients was 91.7% (11/12) , failure rate8.3% (1/11) . After treatment, ALT and AST levels of DAAs treatment patients were decreased (P<0.05) , while CD4 level increased (P <0.05) . Main adverse effects are: 12 patients had gastrointestinal symptoms (22.6%) ; 6 had nausea, vomiting (11.3%) , 4 had diarrhea (7.6%) , 1 had mild rash (1.9%) , and 5 had elevated serum total bilirubin (9.4%) . Conclus ion The overall SVR rate among DAA treatment for HIV/HCV co-infected patients is high (98.1%) with broad indications. Even patients with cirrhosis are eligible. It yielded optimistic outcomes among different gene subtypes, and effectively improved liver function and CD4 level. With oral administration, short regime course, and mild adverse effects, patients can tolerate well, indicating its effectiveness and safety.
Objective To observe the efficacy and safety of DAAs in treating HCV patients with HIV/HCV co-infection.Me thod 53 patients were divided into groups based on HCV genotype. Sofosbuvir + Ledipasvir regime were used for 1 b and 6 a subtypes; sofosbuvir + Daclatasvir regime were used for 3 a, 3 b and those cannot be typed; all the patients diagnosed with cirrhosis were also administrated with ribavirin. The course of treatment for all patients is 12 weeks.Re s ults All 53 patients completed HCV treatment, the overall SVR rate of DAAs treatment rate was 98.1% (52/53) , failure rate 1.9% (1/53) ; SVR rate of DAAs treatment among non-cirrhosis patients was 100% (41/41) ; SVR rate of DAAs treatment among cirrhosis patients was 91.7% (11/12) , failure rate8.3% (1/11) . After treatment, ALT and AST levels of DAAs treatment patients were decreased (P<0.05) , while CD4 level increased (P <0.05) . Main adverse effects are: 12 patients had gastrointestinal symptoms (22.6%) ; 6 had nausea, vomiting (11.3%) , 4 had diarrhea (7.6%) , 1 had mild rash (1.9%) , and 5 had elevated serum total bilirubin (9.4%) . Conclus ion The overall SVR rate among DAA treatment for HIV/HCV co-infected patients is high (98.1%) with broad indications. Even patients with cirrhosis are eligible. It yielded optimistic outcomes among different gene subtypes, and effectively improved liver function and CD4 level. With oral administration, short regime course, and mild adverse effects, patients can tolerate well, indicating its effectiveness and safety.
2018, 39(05): 124-127.
Abstract:
TAR DNA/RNA-binding domain protein 43 (TDP-43) is a highly conserved and widely expressed nuclear protein. TDP-43 is recognized as a pathological marker protein of amyotrophic lateral sclerosis (ALS) , frontotemporal lobar degeneration (FTLD) and alzheimer disease (AD) .This article discusses the structure and the function of TDP-43 and the relationship of its expression in neurodegenerative disease.Meanwhile, this article emphatically probes into the specific expression andfunction of TDP-43 in acute and chronic brain injury based on the knowledge of its biological characteristics, aiming to explore the feasibility for determining the cause of death and the injury and disability situations by TDP-43 in forensic pathology.
TAR DNA/RNA-binding domain protein 43 (TDP-43) is a highly conserved and widely expressed nuclear protein. TDP-43 is recognized as a pathological marker protein of amyotrophic lateral sclerosis (ALS) , frontotemporal lobar degeneration (FTLD) and alzheimer disease (AD) .This article discusses the structure and the function of TDP-43 and the relationship of its expression in neurodegenerative disease.Meanwhile, this article emphatically probes into the specific expression andfunction of TDP-43 in acute and chronic brain injury based on the knowledge of its biological characteristics, aiming to explore the feasibility for determining the cause of death and the injury and disability situations by TDP-43 in forensic pathology.
2018, 39(05): 128-131.
Abstract:
Acquired hemophilia A is a deficiency disease caused by blood coagulation factor VIII antibodies due to a variety of causes, The disease incidence rate is extremely low, but the mortality rate is high. This disease is often misdiagnosed, resulting in delays in treatment, so early diagnosis and timely treatment is crucial for reducing the mortality. The principle of treatment for the disease is mainly to control acute bleeding, eradicate inhibitor, and treat the primary disease, therefore, rational and personalized choices of treatment are also critical.
Acquired hemophilia A is a deficiency disease caused by blood coagulation factor VIII antibodies due to a variety of causes, The disease incidence rate is extremely low, but the mortality rate is high. This disease is often misdiagnosed, resulting in delays in treatment, so early diagnosis and timely treatment is crucial for reducing the mortality. The principle of treatment for the disease is mainly to control acute bleeding, eradicate inhibitor, and treat the primary disease, therefore, rational and personalized choices of treatment are also critical.
2018, 39(05): 132-137.
Abstract:
Objective To evaluate the effect and its factors of the postgraduates' autonomy learning in medical English, and to suggest improving methods. Me thods The postgraduates enrolled in Kunming Medical University in 2016 were divided into two groups. Those for professional degree education learnt Medical English by the approach of autonomy learning, while those for academic degree education were taught in traditional method. At the end of the semester, an examination was applied to measure and compare the learning effects in both groups, and questionnaires were conducted to investigate their attitudes and satisfaction. The results were evaluated by quantitative analysis and qualitative analysis.Re s ults The postgraduates for professional degree education got lower examination scores than the postgraduates for academic degree education. Time management appeared to be the main problem. Conclus ion Although the postgraduates highly regard the necessity of medical English learning and have great sense of learning responsibility, process supervision from the administration is suggested to assist their time management so that better learning effects can be achieved.
Objective To evaluate the effect and its factors of the postgraduates' autonomy learning in medical English, and to suggest improving methods. Me thods The postgraduates enrolled in Kunming Medical University in 2016 were divided into two groups. Those for professional degree education learnt Medical English by the approach of autonomy learning, while those for academic degree education were taught in traditional method. At the end of the semester, an examination was applied to measure and compare the learning effects in both groups, and questionnaires were conducted to investigate their attitudes and satisfaction. The results were evaluated by quantitative analysis and qualitative analysis.Re s ults The postgraduates for professional degree education got lower examination scores than the postgraduates for academic degree education. Time management appeared to be the main problem. Conclus ion Although the postgraduates highly regard the necessity of medical English learning and have great sense of learning responsibility, process supervision from the administration is suggested to assist their time management so that better learning effects can be achieved.
2018, 39(05): 138-140.
Abstract:
Objective To determine the effect of participatory teaching method in the Clinic teaching of internal medicine for MBBS students. Me thods The 60 MBBS students were divided into group A and B, 30 students in each group. Participatory teaching was done in group A, and traditional teaching was carried out in group B, and the effects of the two types of teaching methods were compared. Re s ults In the clinic teaching of internal medicine, the effects in improving students' clinical thinking and practical ability, creativeness, initiative and efficiency were better in the participatory teaching method than in traditional teaching method (P <0.05) .Conclus ion The effects of participatory teaching in the clinic teaching of internal medicine for MBBS students was better than the traditional teaching.
Objective To determine the effect of participatory teaching method in the Clinic teaching of internal medicine for MBBS students. Me thods The 60 MBBS students were divided into group A and B, 30 students in each group. Participatory teaching was done in group A, and traditional teaching was carried out in group B, and the effects of the two types of teaching methods were compared. Re s ults In the clinic teaching of internal medicine, the effects in improving students' clinical thinking and practical ability, creativeness, initiative and efficiency were better in the participatory teaching method than in traditional teaching method (P <0.05) .Conclus ion The effects of participatory teaching in the clinic teaching of internal medicine for MBBS students was better than the traditional teaching.
2018, 39(05): 141-144.
Abstract:
Objective To explore the thinking and methods of integration curriculum. Methods The content of theory and experiment of the biochemistry, molecular biology, cell biology, medical genetics are optimized, streamlined and integrated to form theory course and experiment experimental course of “ basis fo life”, namely“four in two”. Forming a teaching team across departments, multi-disciplinary teaching is carried out. The theory takes “ cell-moleculeinheritance-disorders” as the main line, interspersed with “ flipped class model”. The experimental course deletes repeated confirmatory experiments and adds some comprehensive experiments. The course assessment and performance evaluation are carried out with the combination of formative assessment and summative evaluation. Re s ults The teaching mode of integration curriculum has been carried out in the experimental class of undergraduate students for two years. Compared with the theoretical examination scores, the results of the experimental class were significantly higher than that of the control class, and the difference was statistically significant (P<0.05) . 24 students took part in research in experimental class, but only 10 students did in control class.Conclus ion The implementation of integrated curriculum can promote student's independent learning, improve student's ability to analyze and solve problems, and mobilize student's interest in scientific research practice. It provides useful reference and experience for the integration of basic course in the life science field of higher medical colleges.
Objective To explore the thinking and methods of integration curriculum. Methods The content of theory and experiment of the biochemistry, molecular biology, cell biology, medical genetics are optimized, streamlined and integrated to form theory course and experiment experimental course of “ basis fo life”, namely“four in two”. Forming a teaching team across departments, multi-disciplinary teaching is carried out. The theory takes “ cell-moleculeinheritance-disorders” as the main line, interspersed with “ flipped class model”. The experimental course deletes repeated confirmatory experiments and adds some comprehensive experiments. The course assessment and performance evaluation are carried out with the combination of formative assessment and summative evaluation. Re s ults The teaching mode of integration curriculum has been carried out in the experimental class of undergraduate students for two years. Compared with the theoretical examination scores, the results of the experimental class were significantly higher than that of the control class, and the difference was statistically significant (P<0.05) . 24 students took part in research in experimental class, but only 10 students did in control class.Conclus ion The implementation of integrated curriculum can promote student's independent learning, improve student's ability to analyze and solve problems, and mobilize student's interest in scientific research practice. It provides useful reference and experience for the integration of basic course in the life science field of higher medical colleges.
2018, 39(05): 145-148.
Abstract:
Objective To explore the nursing effects of cognitive behavior nursing model in glaucoma patients and its influence on self-management behavior and long-term efficacy. Me thods 102 cases of glaucoma patients who were admitted to the Department of-2017 in June 2014 July were randomly divided into the control group (n=51 cases) and the observation group (n =51 cases) . The control group received conventional nursing, the observation group with cognitive behavioral nursing mode. The Self-rating Anxiety Scale (SAS) and Self-rating Depression Scale (SDS) were used to evaluate the 2 groups members' psychological and emotional factors. Self Management Behavior Scale was used in the 2 groups at different time points to intervene patients' s self responsibility, health knowledge, self care skills, general grading and self-managing ability, so as to compare the nursing effect of the 2 groups and the long-term curative effects. Re s ults the Self-rating Anxiety Scale and Self-rating Depression Scale scores of the observation group were lower than those of the control group (P<0.05) ; the Self Management Behavior Scores of the observation group after nursing were higher than that of the control group (P<0.05) ; the visual acuity of both groups before nursing was not statistically significant (P >0.05) ; the visual acuities with nursing in the observation group after 3 months, 6 months were higher than the control group (P <0.05) .Conclus ion the cognitive behavior nursing model used in the treatment of glaucoma patients can improve the self-management behavior ability, improve the long-term effect of patients, and is worthy of popularization and application.
Objective To explore the nursing effects of cognitive behavior nursing model in glaucoma patients and its influence on self-management behavior and long-term efficacy. Me thods 102 cases of glaucoma patients who were admitted to the Department of-2017 in June 2014 July were randomly divided into the control group (n=51 cases) and the observation group (n =51 cases) . The control group received conventional nursing, the observation group with cognitive behavioral nursing mode. The Self-rating Anxiety Scale (SAS) and Self-rating Depression Scale (SDS) were used to evaluate the 2 groups members' psychological and emotional factors. Self Management Behavior Scale was used in the 2 groups at different time points to intervene patients' s self responsibility, health knowledge, self care skills, general grading and self-managing ability, so as to compare the nursing effect of the 2 groups and the long-term curative effects. Re s ults the Self-rating Anxiety Scale and Self-rating Depression Scale scores of the observation group were lower than those of the control group (P<0.05) ; the Self Management Behavior Scores of the observation group after nursing were higher than that of the control group (P<0.05) ; the visual acuity of both groups before nursing was not statistically significant (P >0.05) ; the visual acuities with nursing in the observation group after 3 months, 6 months were higher than the control group (P <0.05) .Conclus ion the cognitive behavior nursing model used in the treatment of glaucoma patients can improve the self-management behavior ability, improve the long-term effect of patients, and is worthy of popularization and application.
