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RSS2019 Vol. 40, No. 03
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2019, 40(03): 1-5.
Abstract:
Objective To establish the methamphetamine induced rat conditioned place preference (CPP model, compare the behaviour effects of different time courses of methamphetamine administration and the intervention effect of gastrodin on rat. Methods The rats were intraperitoneally injected (ip) with 10 mg/kg of methamphetamine for 10 days;after confirming the CPP effects were established in rats, the animals of experimental groups were injected with 10 mg/kg of methamphetamine for 7 days, 14 days, and 28 days respectively, to establish three methamphetamine dependent rat model with different dependent times. And then, the gastrodin intervention groups were given 10 mg/kg of gastrodin for 28 days. Then, CPP test were conducted. Results Compared with the control group, the times spent in the drug-paired chamber were increasd significantly in groups of MA-dependent 7 days, 14 days, and 28 days. Moreover, there was statistical difference between 14 days group and 7 days group or 28 days group, but there was no statistical difference between 7 days group and 28 days group.There was significant difference between the MA+Gastrodin groups and the MA groups, and gastrodin intervention groups had shorter time in the drug-paired chamber. Conclusions Constant injection of methamphetamine for 7 days, l4 days, and 28 days can successfully induce CPP effects and establish MA dependent rat model. The CPP effect of methamphetamine 14 days group is more significant than other groups. Gastrodin inervention can alleviate the CPP effect of rat.
Objective To establish the methamphetamine induced rat conditioned place preference (CPP model, compare the behaviour effects of different time courses of methamphetamine administration and the intervention effect of gastrodin on rat. Methods The rats were intraperitoneally injected (ip) with 10 mg/kg of methamphetamine for 10 days;after confirming the CPP effects were established in rats, the animals of experimental groups were injected with 10 mg/kg of methamphetamine for 7 days, 14 days, and 28 days respectively, to establish three methamphetamine dependent rat model with different dependent times. And then, the gastrodin intervention groups were given 10 mg/kg of gastrodin for 28 days. Then, CPP test were conducted. Results Compared with the control group, the times spent in the drug-paired chamber were increasd significantly in groups of MA-dependent 7 days, 14 days, and 28 days. Moreover, there was statistical difference between 14 days group and 7 days group or 28 days group, but there was no statistical difference between 7 days group and 28 days group.There was significant difference between the MA+Gastrodin groups and the MA groups, and gastrodin intervention groups had shorter time in the drug-paired chamber. Conclusions Constant injection of methamphetamine for 7 days, l4 days, and 28 days can successfully induce CPP effects and establish MA dependent rat model. The CPP effect of methamphetamine 14 days group is more significant than other groups. Gastrodin inervention can alleviate the CPP effect of rat.
2019, 40(03): 6-10.
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Objective To explore the expression changes of miRNAs in HRHPV (16 and 18) infected (+) cervical cancer cells after G6 PD expression knock-down. Methods The G6 PD expression in HPV-C33 A, HPV16+Siha and HPV18+Hela cells was inhibited by RNA interference target G6 PD. Human micro RNA Microarray Kit and q RT-PCR were employed to determine the expression changes of mi RNA between different cell lines.Results The expression of G6 PD significantly decreased after RNA interference in different cervical cancer cell lines.Mi RNA microarrayand q RT-PCR analysis revealed that numerousmiRNAs in HPV+ Hela/Siha cells were expressional different from the control HPV-C33 A cells, following G6 PD knockdown. Conclusion The expression changes of various miRNAs following G6 PDknockdown are associated with the oncogenesis events of G6 PD in HR HPV infected cervical cancer cells.
Objective To explore the expression changes of miRNAs in HRHPV (16 and 18) infected (+) cervical cancer cells after G6 PD expression knock-down. Methods The G6 PD expression in HPV-C33 A, HPV16+Siha and HPV18+Hela cells was inhibited by RNA interference target G6 PD. Human micro RNA Microarray Kit and q RT-PCR were employed to determine the expression changes of mi RNA between different cell lines.Results The expression of G6 PD significantly decreased after RNA interference in different cervical cancer cell lines.Mi RNA microarrayand q RT-PCR analysis revealed that numerousmiRNAs in HPV+ Hela/Siha cells were expressional different from the control HPV-C33 A cells, following G6 PD knockdown. Conclusion The expression changes of various miRNAs following G6 PDknockdown are associated with the oncogenesis events of G6 PD in HR HPV infected cervical cancer cells.
2019, 40(03): 11-17.
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Objective To construct an animal model of squamous cell carcinoma of the skin in SKH-1 mice with ultraviolet irradiation, and to investigate the DNA-PKcs activation level of DNA-PKcs-m TORC2/Akt signal transduction pathway during UV-induced skin SCC and AK to SCC transformation. Methods 43 SKH-l hairless mice were randomly divided into experimental group (33) and control group (10) .The experimental group used the SUV-1000 solar simulator to simulate sun-irradiated SKH-1 hairless mice, and the control group did not intervene. Experimental group 1 maintained irradiation for 24 weeks, and experimental group 2 maintained irradiation for 16 weeks. The mice were sacrificed at 12, 18, 24, and 28 weeks for skin histopathological examination. At 28 weeks, the same skin of the same mouse was taken for histopathological examination and immunohistochemistry.Five cases of adult solar keratosis (AK) , cutaneous squamous cell carcinoma (SCC) , normal light-protected site (NNS) , and normal exposed site (NES) were collected for Western Blot. Results After 12 weeks, some mice in the experimental group were thickened in the back skin. At 17 weeks, the papules with diameter ≥ 1 mm appeared in the back skin of experimental group. After 28 weeks of irradiation, the tumor formation rate reached100%. No tumor formation was observed in the control group. Both DNA-PKcs and p-DNA-PKcs (T2609) were localized in the cytoplasm and nucleus. Expression of DNA-PKcs increased in epidermal tissue. There was a difference in the statistical analysis of DNA-PKcs expression (P<0.05) , but there was no difference in the expression of p-DNA-PKcs (T2609) (P>0.05) .The expression levels of DNA-PKcs and p-DNA-PKcs (T2609) in human epidermal tissue increased and there were statistical differences (P<0.05) .Conclusions We successfulyl prepared of mouse AK model and SCC model. It is clear that UV-induced squamous cell carcinoma of the skin can induce DNA damage and repair.
Objective To construct an animal model of squamous cell carcinoma of the skin in SKH-1 mice with ultraviolet irradiation, and to investigate the DNA-PKcs activation level of DNA-PKcs-m TORC2/Akt signal transduction pathway during UV-induced skin SCC and AK to SCC transformation. Methods 43 SKH-l hairless mice were randomly divided into experimental group (33) and control group (10) .The experimental group used the SUV-1000 solar simulator to simulate sun-irradiated SKH-1 hairless mice, and the control group did not intervene. Experimental group 1 maintained irradiation for 24 weeks, and experimental group 2 maintained irradiation for 16 weeks. The mice were sacrificed at 12, 18, 24, and 28 weeks for skin histopathological examination. At 28 weeks, the same skin of the same mouse was taken for histopathological examination and immunohistochemistry.Five cases of adult solar keratosis (AK) , cutaneous squamous cell carcinoma (SCC) , normal light-protected site (NNS) , and normal exposed site (NES) were collected for Western Blot. Results After 12 weeks, some mice in the experimental group were thickened in the back skin. At 17 weeks, the papules with diameter ≥ 1 mm appeared in the back skin of experimental group. After 28 weeks of irradiation, the tumor formation rate reached100%. No tumor formation was observed in the control group. Both DNA-PKcs and p-DNA-PKcs (T2609) were localized in the cytoplasm and nucleus. Expression of DNA-PKcs increased in epidermal tissue. There was a difference in the statistical analysis of DNA-PKcs expression (P<0.05) , but there was no difference in the expression of p-DNA-PKcs (T2609) (P>0.05) .The expression levels of DNA-PKcs and p-DNA-PKcs (T2609) in human epidermal tissue increased and there were statistical differences (P<0.05) .Conclusions We successfulyl prepared of mouse AK model and SCC model. It is clear that UV-induced squamous cell carcinoma of the skin can induce DNA damage and repair.
Experiment Research of Degradable Magnesium Alloy AZ31 Tissue Clips Implanted to New Zealand Rabbits
2019, 40(03): 18-23.
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Objective To study the degradation of magnesium alloy AZ31 tissue clips and its effect on the function of animal organs. Methods New Zealand rabbits were used to build the magnesium alloy AZ31 implanted model. Regular observation of wound healing and general condition was firstly carried out. And then blood was collected to detect the blood routine, coagulation function, liver and kidney functions and the content of calcium, phosphate and magnesium according to the clinical testing methods. The heart, liver, spleen, lung, kidney samples were collected to examine whether pathological changes contained in them. X-ray was carried out to observe the degradation of tissue clips in New Zealand rabbits. Results The biological safety of AZ31 material was confirmed by safety research in vivo. This material had no effect on the results of blood routine index, coagulation function, hepatic function, and renal function cardiac function. No accumulation of harmful substances was observed under the light microscope after HE staining for tissue sections of important internal organs. X-rays showed that there was no significant gas accumulated around the clips and tissue clips degrade slowly in New Zealand rabbitss.Conlusion Magnesium alloy AZ31 is a relatively safe degradable magnesium alloy material, which can be used to make tissue clips, and has a certain clinical application prospect.
Objective To study the degradation of magnesium alloy AZ31 tissue clips and its effect on the function of animal organs. Methods New Zealand rabbits were used to build the magnesium alloy AZ31 implanted model. Regular observation of wound healing and general condition was firstly carried out. And then blood was collected to detect the blood routine, coagulation function, liver and kidney functions and the content of calcium, phosphate and magnesium according to the clinical testing methods. The heart, liver, spleen, lung, kidney samples were collected to examine whether pathological changes contained in them. X-ray was carried out to observe the degradation of tissue clips in New Zealand rabbits. Results The biological safety of AZ31 material was confirmed by safety research in vivo. This material had no effect on the results of blood routine index, coagulation function, hepatic function, and renal function cardiac function. No accumulation of harmful substances was observed under the light microscope after HE staining for tissue sections of important internal organs. X-rays showed that there was no significant gas accumulated around the clips and tissue clips degrade slowly in New Zealand rabbitss.Conlusion Magnesium alloy AZ31 is a relatively safe degradable magnesium alloy material, which can be used to make tissue clips, and has a certain clinical application prospect.
2019, 40(03): 24-28.
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Objective To detect the expression and significance of Beclin-1 and LC3-Ⅱin cervical lesions and HPV infection, and explore their clinical significance. Methods We selected patients in the department of gynecology of the second affiliated hospital of kunming medical university form October 2014 to June 2016 to accept cervical cancer screening (HPV, TCT) , and if any result was abnormal, the patient should be given cervical biopsy. Finally, 40 cases of normal cervical tissues (including 20 cases of HPV infection and 20 cases of no HPV infection) were selected as the disease test result. CINⅠ30 cases; CINⅡ/Ⅲ30 cases; the age range from 25 to 55 years old. The patients who received cervical lesions treatment and pregnant women were not included in the study.Western Blot was used to detect the relative transcript level of Beclin-1, LC3-Ⅱin normal cervical tissues and CINⅠ, CINⅡ/Ⅲ. Results (1) The relative expression levels of Beclin-1 in normal cervical tissue and CIN Ⅰ and CIN Ⅱ/Ⅲ tissuses were (0.561±0.041) , (0.421±0.018) and (0.351±0.016) respectively;The relative expression levels of LC3-Ⅱ in normal cervical tissue and CIN Ⅰ and CIN Ⅱ/Ⅲ tissues were (0.683±0.027) , (0.435±0.029) and (0.325±0.017) . The difference in the relative expression of Beclin-1 and LC3-Ⅱ between the 3 groups were statistically significant (P < 0.001) .The relative expression of Beclin-1 and LC3-II in normal cervical tissues with HPV infection was significantly lower than that in normal cervical tissues without HPV infection (P < 0.05) . Conclusion With the increase of cervical lesion level, autophagy related genes Beclin-1, the relative expression of LC3-Ⅱ is on the decline, suggesting autophagy gene activity decline or loss may be involved in the progress of cervical cancer.
Objective To detect the expression and significance of Beclin-1 and LC3-Ⅱin cervical lesions and HPV infection, and explore their clinical significance. Methods We selected patients in the department of gynecology of the second affiliated hospital of kunming medical university form October 2014 to June 2016 to accept cervical cancer screening (HPV, TCT) , and if any result was abnormal, the patient should be given cervical biopsy. Finally, 40 cases of normal cervical tissues (including 20 cases of HPV infection and 20 cases of no HPV infection) were selected as the disease test result. CINⅠ30 cases; CINⅡ/Ⅲ30 cases; the age range from 25 to 55 years old. The patients who received cervical lesions treatment and pregnant women were not included in the study.Western Blot was used to detect the relative transcript level of Beclin-1, LC3-Ⅱin normal cervical tissues and CINⅠ, CINⅡ/Ⅲ. Results (1) The relative expression levels of Beclin-1 in normal cervical tissue and CIN Ⅰ and CIN Ⅱ/Ⅲ tissuses were (0.561±0.041) , (0.421±0.018) and (0.351±0.016) respectively;The relative expression levels of LC3-Ⅱ in normal cervical tissue and CIN Ⅰ and CIN Ⅱ/Ⅲ tissues were (0.683±0.027) , (0.435±0.029) and (0.325±0.017) . The difference in the relative expression of Beclin-1 and LC3-Ⅱ between the 3 groups were statistically significant (P < 0.001) .The relative expression of Beclin-1 and LC3-II in normal cervical tissues with HPV infection was significantly lower than that in normal cervical tissues without HPV infection (P < 0.05) . Conclusion With the increase of cervical lesion level, autophagy related genes Beclin-1, the relative expression of LC3-Ⅱ is on the decline, suggesting autophagy gene activity decline or loss may be involved in the progress of cervical cancer.
2019, 40(03): 29-34.
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Objective To investigate the prognostic factors of primary hepatocellular carcinoma (HCC after TACE1. Methods The clinical data of 108 patients with primary hepatocellular carcinoma (HCC) from March 2006 to October 2009 in the Department of Interventional Medicine of Yunnan Cancer Hospital were analyzed retrospectively. The Cox regression model was established to explore the influence of various factors on the prognosis of HCC patients treated with TACE. Results The Cox regression model showed that the number of TACE had a significant effect on the survival of HCC patients (P < 0.05) , and the survival risk of the patients undergoing multiple TACE operations was significantly lower than that of the control group. P value of whose cancer located in the whole liver was 0.016, and the survival risk was higher than those distributed only in one liver lobe. P value of NLR> 3.97 was 0.050. Conclusion The number of TACE and the distribution of cancer foci have significant effect on the survival of HCC patients treated with TACE.
Objective To investigate the prognostic factors of primary hepatocellular carcinoma (HCC after TACE1. Methods The clinical data of 108 patients with primary hepatocellular carcinoma (HCC) from March 2006 to October 2009 in the Department of Interventional Medicine of Yunnan Cancer Hospital were analyzed retrospectively. The Cox regression model was established to explore the influence of various factors on the prognosis of HCC patients treated with TACE. Results The Cox regression model showed that the number of TACE had a significant effect on the survival of HCC patients (P < 0.05) , and the survival risk of the patients undergoing multiple TACE operations was significantly lower than that of the control group. P value of whose cancer located in the whole liver was 0.016, and the survival risk was higher than those distributed only in one liver lobe. P value of NLR> 3.97 was 0.050. Conclusion The number of TACE and the distribution of cancer foci have significant effect on the survival of HCC patients treated with TACE.
2019, 40(03): 35-38.
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Objective To observe the variation in PRF before and after taking yunnan baiyao by detecting the concentration of three kinds of cytokines which are Platelet Derived Growth Factors (PDGF-AB) , Transforming Growth Factors (TGF-β) , Insulin-like Growth Factors (IGF) . Methods There were 3 volunteers who were approved by institutional ethical. We collected the venous blood 10 m L from them, in order to prepare for the PRF membrane. Then we put the blood under 37 ℃ and collected their exudates on 1 d, 7 d, 14 d, 21 d, 28 d separately, and used ELISA method to detect the concentration of the exudates. Results (1) The situation of the cytokines releasing: at different time points, TGF-β1 concentration were sable;on day 14 the concentration of PDGF-AB reached the highest, and on day 7 concentration of IGF-1 reached the peak. 2) After taking yunnan baiyao the concentration of cytokines was higher than before observably (P < 0.05) .Conclusion PRF can release the cytokines constantly, taking the yunnan baiyao can stimulate the PRF to release higher concentration of cytokines effectively.
Objective To observe the variation in PRF before and after taking yunnan baiyao by detecting the concentration of three kinds of cytokines which are Platelet Derived Growth Factors (PDGF-AB) , Transforming Growth Factors (TGF-β) , Insulin-like Growth Factors (IGF) . Methods There were 3 volunteers who were approved by institutional ethical. We collected the venous blood 10 m L from them, in order to prepare for the PRF membrane. Then we put the blood under 37 ℃ and collected their exudates on 1 d, 7 d, 14 d, 21 d, 28 d separately, and used ELISA method to detect the concentration of the exudates. Results (1) The situation of the cytokines releasing: at different time points, TGF-β1 concentration were sable;on day 14 the concentration of PDGF-AB reached the highest, and on day 7 concentration of IGF-1 reached the peak. 2) After taking yunnan baiyao the concentration of cytokines was higher than before observably (P < 0.05) .Conclusion PRF can release the cytokines constantly, taking the yunnan baiyao can stimulate the PRF to release higher concentration of cytokines effectively.
2019, 40(03): 39-44.
Abstract:
Objective To study the impact of different personality traits on oral health related life quality (OHRQOL) of adult orthodontic patients in early stage of treatment. Methods According to the Eysenck Personality Questionnaire-Revised short Scale for Chinese (EPQ-RSC) , 82 adult orthodontic patients treated with clear aligner were divided into introvert group, middle group, and extravert group. OHIP-14 questionnaires were collected for 3 times: before treatment (T0) , 1 week after bonding (T1) , 1 month after bonding (T2) to compare the influence of different personality traits on oral health-related quality of life (OHRQOL) for adult orthodontic patients in the early stage of treatment. Results The patient's quality of life was affected to some extent by clear aligner treatment, as the OHRQOL score increased, peaking at 1 week of treatment. Introvert group : the period T2 was decreased in psychological discomfort, psychological disability and the total score which had significant difference compared with the period T0 (P < 0.01) ; the period T2 was decreased in psychological discomfort and the total score which had significant difference compared with the period T1 (P < 0.01) . Extravert group : compared with each other, only the period T0 and T2 had difference in psychological discomfort (P <0.05) . Middle group : the scores of all fields varied in different periods, but there was no statistical significance (P> 0.05) . Patients with different personality traits had differences. The period T0: there was a significant difference in psychological discomfort and the total score between introvert group and middle group (P < 0.01) ; compared introvert group with extravert group, there were significant differences in social disability, disability and the total score (P < 0.01) except for psychological disability; there was a significant difference in physical pain, psychological discomfort and disability between middle group and extravert group (P < 0.01) . The period T1: there was a significant difference only in the field of psychological discomfort between introvert group and middle group (P< 0.01) ;compared introvert group with extravert group, there were differences in physical pain, disability and the total score (P < 0.05) , and the score of the introvert group was significantly higher than that of the extravert group;there was a significant difference only in the field of psychological disability between extravert group and middle group (P < 0.01) . There was no significant difference in psychological discomfort, disability or other fields, among the three groups in T2 period, merely between extravert group and middle group there were significant differences in pain and physiological disorders during this period. Conclusions Clear aligner treatment has impact on the oral health-related quality of life for patients, the impact is related to the personality traits. The treatment has more negative influence for the introvert group on the OHRQOL than the extravert group, mainly in pain and psychological discomfort.
Objective To study the impact of different personality traits on oral health related life quality (OHRQOL) of adult orthodontic patients in early stage of treatment. Methods According to the Eysenck Personality Questionnaire-Revised short Scale for Chinese (EPQ-RSC) , 82 adult orthodontic patients treated with clear aligner were divided into introvert group, middle group, and extravert group. OHIP-14 questionnaires were collected for 3 times: before treatment (T0) , 1 week after bonding (T1) , 1 month after bonding (T2) to compare the influence of different personality traits on oral health-related quality of life (OHRQOL) for adult orthodontic patients in the early stage of treatment. Results The patient's quality of life was affected to some extent by clear aligner treatment, as the OHRQOL score increased, peaking at 1 week of treatment. Introvert group : the period T2 was decreased in psychological discomfort, psychological disability and the total score which had significant difference compared with the period T0 (P < 0.01) ; the period T2 was decreased in psychological discomfort and the total score which had significant difference compared with the period T1 (P < 0.01) . Extravert group : compared with each other, only the period T0 and T2 had difference in psychological discomfort (P <0.05) . Middle group : the scores of all fields varied in different periods, but there was no statistical significance (P> 0.05) . Patients with different personality traits had differences. The period T0: there was a significant difference in psychological discomfort and the total score between introvert group and middle group (P < 0.01) ; compared introvert group with extravert group, there were significant differences in social disability, disability and the total score (P < 0.01) except for psychological disability; there was a significant difference in physical pain, psychological discomfort and disability between middle group and extravert group (P < 0.01) . The period T1: there was a significant difference only in the field of psychological discomfort between introvert group and middle group (P< 0.01) ;compared introvert group with extravert group, there were differences in physical pain, disability and the total score (P < 0.05) , and the score of the introvert group was significantly higher than that of the extravert group;there was a significant difference only in the field of psychological disability between extravert group and middle group (P < 0.01) . There was no significant difference in psychological discomfort, disability or other fields, among the three groups in T2 period, merely between extravert group and middle group there were significant differences in pain and physiological disorders during this period. Conclusions Clear aligner treatment has impact on the oral health-related quality of life for patients, the impact is related to the personality traits. The treatment has more negative influence for the introvert group on the OHRQOL than the extravert group, mainly in pain and psychological discomfort.
2019, 40(03): 45-48.
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Objective To evaluate the clinical curative effect of improved FP-7 Ahmed glaucoma valve (AGV) implantation for treating traumatic secondary glaucoma. Methods We used retrospective case series study.We collected a total of 32 eyes of 32 patients with traumatic secondary glaucoma patients who underwent improved FP-7 Ahmed glaucoma valve implantation, the change of intraocular pressure (IOP) , the best corrected visual acuity (BCVA) , success rate and early postoperative complications were observed 24 months after surgery. Results There were many factors causing the traumatic secondary glaucoma, and there were many combined factors. Compared to preoperation, the BCVA was improved in 10 eyes, remained the same in 15 eyes, and decreased in 7 eyes. IOP was significantly decreased after surgery with improved FP-7 AGV implantation (P<0.05) , (38.8 ± 9.2) mm Hg before surgery vs (13.2±4.5) mm Hg, (15.7±4.8) mm Hg, (16.2±5.2) mm Hg, (17.7±6.7) mm Hg and (18.6±7.8) mm Hg at the 1, 3, 6, 12, 24 months after surgery. The total success rate of AGV implantation at the 1, 3, 6, 12, 24 months after surgery was 100%, 93.75%, 90.63%, 86.05%, 76.67%. Conclusion Improved FP-7 AGV implantation is safe and effective, to improve surgeons' operative technique may reduce the complications
Objective To evaluate the clinical curative effect of improved FP-7 Ahmed glaucoma valve (AGV) implantation for treating traumatic secondary glaucoma. Methods We used retrospective case series study.We collected a total of 32 eyes of 32 patients with traumatic secondary glaucoma patients who underwent improved FP-7 Ahmed glaucoma valve implantation, the change of intraocular pressure (IOP) , the best corrected visual acuity (BCVA) , success rate and early postoperative complications were observed 24 months after surgery. Results There were many factors causing the traumatic secondary glaucoma, and there were many combined factors. Compared to preoperation, the BCVA was improved in 10 eyes, remained the same in 15 eyes, and decreased in 7 eyes. IOP was significantly decreased after surgery with improved FP-7 AGV implantation (P<0.05) , (38.8 ± 9.2) mm Hg before surgery vs (13.2±4.5) mm Hg, (15.7±4.8) mm Hg, (16.2±5.2) mm Hg, (17.7±6.7) mm Hg and (18.6±7.8) mm Hg at the 1, 3, 6, 12, 24 months after surgery. The total success rate of AGV implantation at the 1, 3, 6, 12, 24 months after surgery was 100%, 93.75%, 90.63%, 86.05%, 76.67%. Conclusion Improved FP-7 AGV implantation is safe and effective, to improve surgeons' operative technique may reduce the complications
Changes of Cellular Immune Function in Chronic Obstructive Pulmonary Disease Patients with Infection
2019, 40(03): 49-52.
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Objective To explore the changes of cellular immune function in chronic obstructive pulmonary disease patients with infection, and to provide a theoretical basis for COPD patients. Methods Totally 80 patients with COPD complicating infection treated in our hospital from January 2016 to April 2017 were selected as the observation group, contemporaneous 64 patients with simple COPD were selected as the COPD group and 60 healthy people as the control group. The indexes of cellular immunity functions were analyzed in 3 groups. Results (1) In COPD infection group, the average percentage of peripheral blood NK was 19.86 + 10.67, and lower than the COPD group (26.47±10.05) , the difference was statistically significant (P < 0.01) , higher than that of the control group (16.89±5.07) , but the difference was not statistically significant (P > 0.05) . (2) CD3+ cells in peripheral blood of COPD patients with bacterial infection group, COPD group and healthy control group (total T lymphocyte percentage) were (66.64±11.03) , (64.6±11.45) and (67.06±5.55) , there was no significant difference between the three groups (P > 0.05) . (3) In COPD infection group, the peripheral blood CD4 cells and CD4/CD8 ratio were (40.47±10.44) and 1.82 (1.78, 2.57) , higher than the healthy control group[35.01 +7.16 and 1.19 (0.96, 1.66) ], the level of CD8 cells was (23.6±9.82) , lower than the healthy control group (27.50±5.85) , the differences were statistically significant (P < 0.01) ;and COPD group level[ (39.89±9.92) , 2.02 (1.44, 2.83) ] and (21.12±7.64) , there were no statistically significant difference. Conclusions In patients with COPD, the adjuvant immune cell levels are increased but the specific killing immune cells are decreased, on this basis, increased levels of nonspecific killing immune cells help fight infection, immune regulation therapy for COPD patients should focus on improving the level of destruction of immune cells.
Objective To explore the changes of cellular immune function in chronic obstructive pulmonary disease patients with infection, and to provide a theoretical basis for COPD patients. Methods Totally 80 patients with COPD complicating infection treated in our hospital from January 2016 to April 2017 were selected as the observation group, contemporaneous 64 patients with simple COPD were selected as the COPD group and 60 healthy people as the control group. The indexes of cellular immunity functions were analyzed in 3 groups. Results (1) In COPD infection group, the average percentage of peripheral blood NK was 19.86 + 10.67, and lower than the COPD group (26.47±10.05) , the difference was statistically significant (P < 0.01) , higher than that of the control group (16.89±5.07) , but the difference was not statistically significant (P > 0.05) . (2) CD3+ cells in peripheral blood of COPD patients with bacterial infection group, COPD group and healthy control group (total T lymphocyte percentage) were (66.64±11.03) , (64.6±11.45) and (67.06±5.55) , there was no significant difference between the three groups (P > 0.05) . (3) In COPD infection group, the peripheral blood CD4 cells and CD4/CD8 ratio were (40.47±10.44) and 1.82 (1.78, 2.57) , higher than the healthy control group[35.01 +7.16 and 1.19 (0.96, 1.66) ], the level of CD8 cells was (23.6±9.82) , lower than the healthy control group (27.50±5.85) , the differences were statistically significant (P < 0.01) ;and COPD group level[ (39.89±9.92) , 2.02 (1.44, 2.83) ] and (21.12±7.64) , there were no statistically significant difference. Conclusions In patients with COPD, the adjuvant immune cell levels are increased but the specific killing immune cells are decreased, on this basis, increased levels of nonspecific killing immune cells help fight infection, immune regulation therapy for COPD patients should focus on improving the level of destruction of immune cells.
2019, 40(03): 53-57.
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Objective To retrospectively analyze clinical distribution and drug resistance of pseudomonas aeruginosa infection in patients of different ages and provide reference for rational use of antibiotics in clinic.Methods Pseudomonas aeruginosa clinically isolated from yuxi people's hospital from January to December 2016 was collected for drug sensitivity test, and its clinical distribution and drug resistance characteristics were studied.Results A total of 282 strains of pseudomonas aeruginosa were isolated from clinical specimens, accounting for2.98% of the total clinical isolation. The infection rate of elderly patients over 70 years old accounted for a large proportion (41.5%) , and the infection rate of young people was relatively lower (3.2%) . The infection was mainly in respiratory tract, accounting for 80.9%, most of which were derived from sputum, accounting for77.7%, followed by secretions and urine. The antimicrobial resistance of pseudomonas aeruginosa varies with the age and site of infection, From the age distribution, piperacillin, ceftazidime, cefepime, imipenem and meropenem had significant differences (P < 0.05) , The drug resistance rate of elderly patients was generally higher, and the drug resistance rate of levofloxacin was higher than other ages in the 31-50 age group. From the different specimens, except imipenem and furantoin, the drug resistance of the antibiotics monitored was different (P < 0.05) , The drug resistance rate of isolates from secretion samples was the lowest among the four groups.The drug resistance rate of isolated strains in urine specimens was significantly higher than that of other specimens.Conclusion The drug resistance rate of pseudomonas aeruginosa in this hospital is different because of the different clinical distribution, and the drug susceptibility test should be used for the rational use of antibiotics and to strengthen the monitoring of drug resistance to prevent and reduce the occurrence of multiple drug resistance and infection in the hospital.
Objective To retrospectively analyze clinical distribution and drug resistance of pseudomonas aeruginosa infection in patients of different ages and provide reference for rational use of antibiotics in clinic.Methods Pseudomonas aeruginosa clinically isolated from yuxi people's hospital from January to December 2016 was collected for drug sensitivity test, and its clinical distribution and drug resistance characteristics were studied.Results A total of 282 strains of pseudomonas aeruginosa were isolated from clinical specimens, accounting for2.98% of the total clinical isolation. The infection rate of elderly patients over 70 years old accounted for a large proportion (41.5%) , and the infection rate of young people was relatively lower (3.2%) . The infection was mainly in respiratory tract, accounting for 80.9%, most of which were derived from sputum, accounting for77.7%, followed by secretions and urine. The antimicrobial resistance of pseudomonas aeruginosa varies with the age and site of infection, From the age distribution, piperacillin, ceftazidime, cefepime, imipenem and meropenem had significant differences (P < 0.05) , The drug resistance rate of elderly patients was generally higher, and the drug resistance rate of levofloxacin was higher than other ages in the 31-50 age group. From the different specimens, except imipenem and furantoin, the drug resistance of the antibiotics monitored was different (P < 0.05) , The drug resistance rate of isolates from secretion samples was the lowest among the four groups.The drug resistance rate of isolated strains in urine specimens was significantly higher than that of other specimens.Conclusion The drug resistance rate of pseudomonas aeruginosa in this hospital is different because of the different clinical distribution, and the drug susceptibility test should be used for the rational use of antibiotics and to strengthen the monitoring of drug resistance to prevent and reduce the occurrence of multiple drug resistance and infection in the hospital.
2019, 40(03): 58-62.
Abstract:
Objective To compare the clinical efficacy of percutaneous pedicle screw fixation with conventional open spinal internal fixation for type A3 thoracolumbar fractures. Methods The retrospective study consisted of 86 patients who received minimally invasive fixation or open fixation. Patients were between 15 and 56 years of age, had a thoracolumbar fracture and no neurological deficits. Kyphotic angle (Cobb angle) and loss of reduction was compared after minimal invasive and open fixation. The Cobb angle was evaluated directly postoperatively, at 1 week, 6 months and 12 months after surgery. Results Patients who received the minimally invasive fixation had a shorter surgical intervention time and a shorter hospital stay compared to patients who received the open fixation. There was no significant difference in the local Cobb angle between the two groups; the minimally invasive group was significantly different than the open group in the loss of the anterior border of the vertebral body.Conclusions In this study we provide evidence that percutaneous pedicle screw can effectively treat type A3 thoracolumbar fractures with less bleeding, less trauma and quick recovery. This method acquires satisfactory clinical results in long-term follow-up, especially for young patients.
Objective To compare the clinical efficacy of percutaneous pedicle screw fixation with conventional open spinal internal fixation for type A3 thoracolumbar fractures. Methods The retrospective study consisted of 86 patients who received minimally invasive fixation or open fixation. Patients were between 15 and 56 years of age, had a thoracolumbar fracture and no neurological deficits. Kyphotic angle (Cobb angle) and loss of reduction was compared after minimal invasive and open fixation. The Cobb angle was evaluated directly postoperatively, at 1 week, 6 months and 12 months after surgery. Results Patients who received the minimally invasive fixation had a shorter surgical intervention time and a shorter hospital stay compared to patients who received the open fixation. There was no significant difference in the local Cobb angle between the two groups; the minimally invasive group was significantly different than the open group in the loss of the anterior border of the vertebral body.Conclusions In this study we provide evidence that percutaneous pedicle screw can effectively treat type A3 thoracolumbar fractures with less bleeding, less trauma and quick recovery. This method acquires satisfactory clinical results in long-term follow-up, especially for young patients.
2019, 40(03): 63-67.
Abstract:
Objective To discuss the characteristics and efficacy evaluation of the different devices guided arterial temporary balloon occlusion technique in dangerous placenta previa caesarean delivery. Methods The patients who had visited obstetrics department as total 26 were collected from July 2016 to August 2018. All patients were diagnosed as dangerous placenta previa by ultrasound or (and) magnetic resonance imaging (MRI) and had been received the arterial temporary balloon occlusion pre-caesarean delivery. Guided with different devices, the balloon was pre-embedded in the bilateral internal or common iliac artery. Immediately blocked the blood flow of iliac artery after the fetal delivered. Withdrawn the balloon according to the obstetric operation progression and bleeding.Results All the 26 patients were succeful in the arterial temporary balloon pre-embedded, and 1 patient of which taken embolotherapy 1 patient due to pseudo-aneurysm formation of right uterine artery. The occlusion time was about 3-15 mins while the intra-operation bleeding amount was about 400-1000 m L in all the patients. There was no hysterectomy or post-operative blood transfusion. All the newborns were survival. Conclusion The arterial temporary balloon occlusion technique in dangerous placenta previa caesarean delivery can decrease the amount of bleeding during the caesarean delivery and the incidence of related complication. It has advantages such as micro-invasion, strong safety and curative effect, and has been widely used in clinic.
Objective To discuss the characteristics and efficacy evaluation of the different devices guided arterial temporary balloon occlusion technique in dangerous placenta previa caesarean delivery. Methods The patients who had visited obstetrics department as total 26 were collected from July 2016 to August 2018. All patients were diagnosed as dangerous placenta previa by ultrasound or (and) magnetic resonance imaging (MRI) and had been received the arterial temporary balloon occlusion pre-caesarean delivery. Guided with different devices, the balloon was pre-embedded in the bilateral internal or common iliac artery. Immediately blocked the blood flow of iliac artery after the fetal delivered. Withdrawn the balloon according to the obstetric operation progression and bleeding.Results All the 26 patients were succeful in the arterial temporary balloon pre-embedded, and 1 patient of which taken embolotherapy 1 patient due to pseudo-aneurysm formation of right uterine artery. The occlusion time was about 3-15 mins while the intra-operation bleeding amount was about 400-1000 m L in all the patients. There was no hysterectomy or post-operative blood transfusion. All the newborns were survival. Conclusion The arterial temporary balloon occlusion technique in dangerous placenta previa caesarean delivery can decrease the amount of bleeding during the caesarean delivery and the incidence of related complication. It has advantages such as micro-invasion, strong safety and curative effect, and has been widely used in clinic.
2019, 40(03): 68-72.
Abstract:
Objective To investigate whether the transcatheter closure of funnel shaped patent ductus arteriosus (PDA) under the guidance of transthoracic ultrasound can be used as a modified treatment method for traditional PDA closure, so as to shorten the time for operation, patients and operators under the X-ray, and to generalize the method. Methods There were 115 FDA cases of 1-14 year-old with an average in 3.5, who were chosen from September 2015 to September 2017 of the First Affiliated Hospital of Kunming Medical University, which were excluded from cardiovascular malformations and divided into observation group by 66 cases in random, and the rest 49 cases were selected as control group. Traditional descending aortographies and the X-ray guiding were applied to the control group. Results The accuracy of the experimental group was higher than that of the control group when the narrowest arterial duct rages from 2 mm to 8 mm, the differences was statically significant (P <0.05) . Also, the incidence of operative complications was statistically significant (P < 0.05) . Conclusion TTE guided PDA closure has the advantages of simple operating, time saving, without contrast media using, safety and reliability, wide operation and low cost, it has great potential for development and broad application.
Objective To investigate whether the transcatheter closure of funnel shaped patent ductus arteriosus (PDA) under the guidance of transthoracic ultrasound can be used as a modified treatment method for traditional PDA closure, so as to shorten the time for operation, patients and operators under the X-ray, and to generalize the method. Methods There were 115 FDA cases of 1-14 year-old with an average in 3.5, who were chosen from September 2015 to September 2017 of the First Affiliated Hospital of Kunming Medical University, which were excluded from cardiovascular malformations and divided into observation group by 66 cases in random, and the rest 49 cases were selected as control group. Traditional descending aortographies and the X-ray guiding were applied to the control group. Results The accuracy of the experimental group was higher than that of the control group when the narrowest arterial duct rages from 2 mm to 8 mm, the differences was statically significant (P <0.05) . Also, the incidence of operative complications was statistically significant (P < 0.05) . Conclusion TTE guided PDA closure has the advantages of simple operating, time saving, without contrast media using, safety and reliability, wide operation and low cost, it has great potential for development and broad application.
2019, 40(03): 73-77.
Abstract:
Objective To investigate the effect of three doses of bupivacaine in caesarean section under combined spinal-epidural anesthesia for parturients with normal body mass. Methods From June 2017 to July2018, 150 parturients undergoing cesarean section in Yan'an Hospital affiliated to Kunming Medical University were divided into group A, B and C (n = 50 cases) . Combined spinal-epidural anesthesia was performed in L3-4 space, 0.75% bupivacaine injection 10 mg, 11.5 mg and 12.5 mg were respectively injected into the subarachnoid, and the parturient were assisted to take a horizontal position after placing epidural tube 4 cm on the head side. The anesthesia sensation blocking plane was measured every 2 min. HR, Sp O2 and BP were recorded, and the occurrence of adverse reactions such as hypotension, bradycardia, tachycardia, nausea, vomiting and chest distress was closely observed and handled in time. Results There was no significant difference in age, height, weight and BMI (kg/m2) between the three groups (P > 0.05) . The operation time in group A was significantly longer than that in groups B and C (P < 0.05) , and the anesthesia onset time, analgesic effect and muscle relaxation effect in groups B and C were better than those in group A (P < 0.05) . MBP in group C was significantly lower than that in group A and B after the fetus was taken out and at the end of the operation, while HR in 5 minutes after injection was significantly higher than that in group A and B ( P < 0.05) . There was no significant difference in hypotension, nausea and vomiting and the use of antihypertensive drugs in the three groups.Conclusion Under the condition that the drug concentration, injection speed and puncture site selection are unchanged, according to the method of 0.75 % bupivacaine 2 m L plus cerebrospinal fluid 1 ml, 11.5 mg (2.3 m L) of bupivacaine for combined spinal-epidural anesthesia for parturients with normal body mass may be the most appropriate and safe dose in clinical anesthesia.
Objective To investigate the effect of three doses of bupivacaine in caesarean section under combined spinal-epidural anesthesia for parturients with normal body mass. Methods From June 2017 to July2018, 150 parturients undergoing cesarean section in Yan'an Hospital affiliated to Kunming Medical University were divided into group A, B and C (n = 50 cases) . Combined spinal-epidural anesthesia was performed in L3-4 space, 0.75% bupivacaine injection 10 mg, 11.5 mg and 12.5 mg were respectively injected into the subarachnoid, and the parturient were assisted to take a horizontal position after placing epidural tube 4 cm on the head side. The anesthesia sensation blocking plane was measured every 2 min. HR, Sp O2 and BP were recorded, and the occurrence of adverse reactions such as hypotension, bradycardia, tachycardia, nausea, vomiting and chest distress was closely observed and handled in time. Results There was no significant difference in age, height, weight and BMI (kg/m2) between the three groups (P > 0.05) . The operation time in group A was significantly longer than that in groups B and C (P < 0.05) , and the anesthesia onset time, analgesic effect and muscle relaxation effect in groups B and C were better than those in group A (P < 0.05) . MBP in group C was significantly lower than that in group A and B after the fetus was taken out and at the end of the operation, while HR in 5 minutes after injection was significantly higher than that in group A and B ( P < 0.05) . There was no significant difference in hypotension, nausea and vomiting and the use of antihypertensive drugs in the three groups.Conclusion Under the condition that the drug concentration, injection speed and puncture site selection are unchanged, according to the method of 0.75 % bupivacaine 2 m L plus cerebrospinal fluid 1 ml, 11.5 mg (2.3 m L) of bupivacaine for combined spinal-epidural anesthesia for parturients with normal body mass may be the most appropriate and safe dose in clinical anesthesia.
2019, 40(03): 78-82.
Abstract:
Objective To provide evidences for early intervention in chidren with brain injury syndrome through studing the hearing characteristics and influencing factors in these children. Methods A total of 466 children with brain injury syndrome were selected who visited Kunming children's Hospital from June 2017 to June2018. All children were examined by brainstem auditory evoked potential (BAEP) . The premature infants group (34-37 weeks, 31-34 weeks, < 31 weeks threegroups) , hypoxia group (HIE and non-HIEtwo groups) and jaundice group (nuclear jaundice and non-nuclear jaundice twogroups) were analyzed statistically to compare the difference of hearing impairment rate and severity between different degreeof risk factors.Result The abnormal detection rate of BAEP was the highest in the nuclear jaundice group (51.9%) .There was no significant difference in the abnormal rate of BAEP among three groups of preterm infants of different gestational age (χ2= 5.826, P >0.05) , but there was significant difference in the degree of hearing impairment (Z = 11.837, P < 0.01) .The smaller the gestational age, the more serious the hearing damage. There was no significant difference in the abnormal rate of BAEP between HIE group and non HIE group ( χ2= 3.66, P > 0.05) , but there was significant difference in the degree of hearing impairment (Z =-2.107, P < 0.05) , hearing impairment in HIE group was more serious than that in non-HIE group. The abnormal rate of BAEP and the degree of hearing impairment were significantly different between the nuclear jaundice and non-nuclear jaundice. The abnormal rate of BAEP was higher and the degree of hearing impairment was more serious in the group of nuclear jaundice. (χ2= 22.286, P <0.01, Z =-2.666, P < 0.01) .Conclusions In the children with brain injury syndrome, the smaller the week of premature delivery, HIE and the nuclear jaundice, the more serious degree of hearing impairment. In children with hyper bilirubinemia, the rate of abnormal hearing in nuclear jaundice is higher than that in children without nuclear jaundice. Therefore, BAEP test should be performed as soon as possible to assess the hearing status of children with the above risk factors.
Objective To provide evidences for early intervention in chidren with brain injury syndrome through studing the hearing characteristics and influencing factors in these children. Methods A total of 466 children with brain injury syndrome were selected who visited Kunming children's Hospital from June 2017 to June2018. All children were examined by brainstem auditory evoked potential (BAEP) . The premature infants group (34-37 weeks, 31-34 weeks, < 31 weeks threegroups) , hypoxia group (HIE and non-HIEtwo groups) and jaundice group (nuclear jaundice and non-nuclear jaundice twogroups) were analyzed statistically to compare the difference of hearing impairment rate and severity between different degreeof risk factors.Result The abnormal detection rate of BAEP was the highest in the nuclear jaundice group (51.9%) .There was no significant difference in the abnormal rate of BAEP among three groups of preterm infants of different gestational age (χ2= 5.826, P >0.05) , but there was significant difference in the degree of hearing impairment (Z = 11.837, P < 0.01) .The smaller the gestational age, the more serious the hearing damage. There was no significant difference in the abnormal rate of BAEP between HIE group and non HIE group ( χ2= 3.66, P > 0.05) , but there was significant difference in the degree of hearing impairment (Z =-2.107, P < 0.05) , hearing impairment in HIE group was more serious than that in non-HIE group. The abnormal rate of BAEP and the degree of hearing impairment were significantly different between the nuclear jaundice and non-nuclear jaundice. The abnormal rate of BAEP was higher and the degree of hearing impairment was more serious in the group of nuclear jaundice. (χ2= 22.286, P <0.01, Z =-2.666, P < 0.01) .Conclusions In the children with brain injury syndrome, the smaller the week of premature delivery, HIE and the nuclear jaundice, the more serious degree of hearing impairment. In children with hyper bilirubinemia, the rate of abnormal hearing in nuclear jaundice is higher than that in children without nuclear jaundice. Therefore, BAEP test should be performed as soon as possible to assess the hearing status of children with the above risk factors.
2019, 40(03): 83-86.
Abstract:
Objective To investigate the effect of acute hypervolemic hemodilution on hemodynamics in patients undergoing lumbar surgery in prone position. Methods 150 patients in prone position were randomly divided into 3 groups: Ringer's lactate solution group (n=50) : Ringer's lactate solution 15 m L/kg was injected within 30 minutes after anesthesia induction; hydroxyethyl starch group 1 (n=50) ;hydroxyethyl starch sodium chloride (130/0.4) 15 ml/kg was injected within 30 minutes after anesthesia induction; hydroxyethyl starch group 2 (n=50) : after induction of anesthesia, hydroxyethyl starch sodium chloride (130/0.4) 20 ml/kg was injected into 30 min. Flo Trac/Vigilo cardiac output monitor was used to continuously monitor the patients' SVI, CI, SVV, MAP, HR, CI, SVI, CVP and CVP in supine and prone positions. Airway pressure (P) . Results Compared with the supine position, the CI, SVI and CVP in RS group decreased significantly 10 minutes after prone position (P < 0.05) , MAP, SVV and airway pressure increased significantly (P < 0.05) , HR did not change significantly (P > 0.05) ;the change trend of the above parameters in HES1 group was the same as that in RS group, but the difference of parameters between prone position and supine position was more obvious than that in RS group. There was no significant difference in CI, SVI, HR, SVV and supine position after prone position in HES2 group (P > 0.05) , MAP and airway pressure after prone position were significantly higher than those in supine position (P < 0.05) . Conclusion Acute hemodilution (HES 15 m L/kg and 20 m L/kg) can improve the hemodynamic fluctuation after prone position, and HES 20 ml/kg is more beneficial to maintain the circulation stability after prone position.
Objective To investigate the effect of acute hypervolemic hemodilution on hemodynamics in patients undergoing lumbar surgery in prone position. Methods 150 patients in prone position were randomly divided into 3 groups: Ringer's lactate solution group (n=50) : Ringer's lactate solution 15 m L/kg was injected within 30 minutes after anesthesia induction; hydroxyethyl starch group 1 (n=50) ;hydroxyethyl starch sodium chloride (130/0.4) 15 ml/kg was injected within 30 minutes after anesthesia induction; hydroxyethyl starch group 2 (n=50) : after induction of anesthesia, hydroxyethyl starch sodium chloride (130/0.4) 20 ml/kg was injected into 30 min. Flo Trac/Vigilo cardiac output monitor was used to continuously monitor the patients' SVI, CI, SVV, MAP, HR, CI, SVI, CVP and CVP in supine and prone positions. Airway pressure (P) . Results Compared with the supine position, the CI, SVI and CVP in RS group decreased significantly 10 minutes after prone position (P < 0.05) , MAP, SVV and airway pressure increased significantly (P < 0.05) , HR did not change significantly (P > 0.05) ;the change trend of the above parameters in HES1 group was the same as that in RS group, but the difference of parameters between prone position and supine position was more obvious than that in RS group. There was no significant difference in CI, SVI, HR, SVV and supine position after prone position in HES2 group (P > 0.05) , MAP and airway pressure after prone position were significantly higher than those in supine position (P < 0.05) . Conclusion Acute hemodilution (HES 15 m L/kg and 20 m L/kg) can improve the hemodynamic fluctuation after prone position, and HES 20 ml/kg is more beneficial to maintain the circulation stability after prone position.
2019, 40(03): 87-91.
Abstract:
Objective To investigate the expression and clinical significance of LAPTM4 B in endometrial carcinoma. Methods A total of 76 cases of patients with endometrial carcinoma who underwent surgery were selected in our hospital from March 2011 to March 2013. In the same period, 40 cases of normal endometrial tissues were selected as the control group. The expressions of LAPTM4 B proteins in endometrial carcinoma and control group tissues were detected by immunohistochemistry. All patients were followed up, and the follow-up deadline was March 31, 2018. Four patients were lost during the follow-up period, and the follow-up rate was 93.42%. Survival analysis was using Kaplan-Meier method and Log-Rank test. Multivariate analysis of factors affecting patient prognosis was using Cox proportional hazard regression model. Results The positive expression rate of LAPTM4 B protein in endometrial carcinoma tissues was 73.68%, which was higher than that in control group (27.50%) , the difference was statistically significant (χ2= 22.911, P < 0.001) . The differences of the expression of LAPTM4 B proteins in different FIGO stages, different degrees of differentiation and lymph node metastasis were statistically significant (P < 0.05) . Kaplan-Meier survival analysis showed that the average survival time in the positive expression group was (42.76±4.16) months, the survival rate was 33.96%, while in the negative expression group were (71.53 ±4.78) months and 78.95%, respectively, Log-Rank test showed the difference was statistically significant (χ2= 10.458, P = 0.001) . Cox proportional hazards regression showed that the degree of differentiation, lymph node metastasis and LAPTM4 B protein expression were factors influencing the prognosis of patients (HR=0.436, 5.993 and 3.052, P < 0.05) . Conclusions The expression of LAPTM4 B protein in endometrial carcinoma tissues is upregulated, and is related to the malignant progression of the tumor and the prognosis. It is a risk factor affecting the prognosis of patients.
Objective To investigate the expression and clinical significance of LAPTM4 B in endometrial carcinoma. Methods A total of 76 cases of patients with endometrial carcinoma who underwent surgery were selected in our hospital from March 2011 to March 2013. In the same period, 40 cases of normal endometrial tissues were selected as the control group. The expressions of LAPTM4 B proteins in endometrial carcinoma and control group tissues were detected by immunohistochemistry. All patients were followed up, and the follow-up deadline was March 31, 2018. Four patients were lost during the follow-up period, and the follow-up rate was 93.42%. Survival analysis was using Kaplan-Meier method and Log-Rank test. Multivariate analysis of factors affecting patient prognosis was using Cox proportional hazard regression model. Results The positive expression rate of LAPTM4 B protein in endometrial carcinoma tissues was 73.68%, which was higher than that in control group (27.50%) , the difference was statistically significant (χ2= 22.911, P < 0.001) . The differences of the expression of LAPTM4 B proteins in different FIGO stages, different degrees of differentiation and lymph node metastasis were statistically significant (P < 0.05) . Kaplan-Meier survival analysis showed that the average survival time in the positive expression group was (42.76±4.16) months, the survival rate was 33.96%, while in the negative expression group were (71.53 ±4.78) months and 78.95%, respectively, Log-Rank test showed the difference was statistically significant (χ2= 10.458, P = 0.001) . Cox proportional hazards regression showed that the degree of differentiation, lymph node metastasis and LAPTM4 B protein expression were factors influencing the prognosis of patients (HR=0.436, 5.993 and 3.052, P < 0.05) . Conclusions The expression of LAPTM4 B protein in endometrial carcinoma tissues is upregulated, and is related to the malignant progression of the tumor and the prognosis. It is a risk factor affecting the prognosis of patients.
2019, 40(03): 92-96.
Abstract:
Objective To study the effects of amphotericin B irrigation on eosinophils (Eos) and epithelization time in patients with refractory rhinosinusitis after nasal endoscopic surgery. Methods The random number table method was performed to divided the 86 patients with refractory rhinosinusitis undergoing nasal endoscopic surgery in Otolaryngology&Head and Neck Surgery, Jinzhou Central Hospital, into two groups, 43 cases in each group. The control group was given routine treatment after surgery, while the study group was treated with amphotericin B irrigation on the basis of routine treatment. The T lymphocyte subsets (CD3+, CD4+, CD8+and CD4+/CD8+) , Eos count, inflammatory factors [interleukin 2 (IL-2) , interleukin 4 (IL-4) , interleukin5 (IL-5) , serum interferon γ (IFN-γ) ], epithelization time, postoperative recurrence rate, swelling degree and curative effect were compared between the two groups. Results The total response rate of postoperative curative effect in the study group was significantly higher than that in the control group (95.35% vs 81.40%) (P <0.05) . At 1 month after surgery, CD3+, CD4+, CD8+and CD4+/CD8+in the study group were significantly higher than those in the control group (P < 0.05) , while Eos count, levels of IL-2, IL-4, IL-5 and IFN-γ were significant lower than those in the control group (P < 0.05) . At 7 days after surgery, the VAS score and severe swelling rate in the study group were significantly lower than those in the control group (P < 0.05) . The epithelization time was shorter than that in the control group (P < 0.05) . The recurrence rate in the study group was significantly lower than that in the control group (P < 0.05) . Conclusion The application of amphotericin B irrigation in patients with refractory rhinosinusitis after nasal endoscopic surgery can effectively enhance the immune function, improve inflammation, relieve degree of swelling and pain, reduce Eos, shorten the epithelization time, and reduce the postoperative recurrence rate.
Objective To study the effects of amphotericin B irrigation on eosinophils (Eos) and epithelization time in patients with refractory rhinosinusitis after nasal endoscopic surgery. Methods The random number table method was performed to divided the 86 patients with refractory rhinosinusitis undergoing nasal endoscopic surgery in Otolaryngology&Head and Neck Surgery, Jinzhou Central Hospital, into two groups, 43 cases in each group. The control group was given routine treatment after surgery, while the study group was treated with amphotericin B irrigation on the basis of routine treatment. The T lymphocyte subsets (CD3+, CD4+, CD8+and CD4+/CD8+) , Eos count, inflammatory factors [interleukin 2 (IL-2) , interleukin 4 (IL-4) , interleukin5 (IL-5) , serum interferon γ (IFN-γ) ], epithelization time, postoperative recurrence rate, swelling degree and curative effect were compared between the two groups. Results The total response rate of postoperative curative effect in the study group was significantly higher than that in the control group (95.35% vs 81.40%) (P <0.05) . At 1 month after surgery, CD3+, CD4+, CD8+and CD4+/CD8+in the study group were significantly higher than those in the control group (P < 0.05) , while Eos count, levels of IL-2, IL-4, IL-5 and IFN-γ were significant lower than those in the control group (P < 0.05) . At 7 days after surgery, the VAS score and severe swelling rate in the study group were significantly lower than those in the control group (P < 0.05) . The epithelization time was shorter than that in the control group (P < 0.05) . The recurrence rate in the study group was significantly lower than that in the control group (P < 0.05) . Conclusion The application of amphotericin B irrigation in patients with refractory rhinosinusitis after nasal endoscopic surgery can effectively enhance the immune function, improve inflammation, relieve degree of swelling and pain, reduce Eos, shorten the epithelization time, and reduce the postoperative recurrence rate.
2019, 40(03): 97-101.
Abstract:
Objective To analyze the clinicopathological features of pancreatic cancer and the risk factors affecting the prognosis. Methods The clinical data and follow-up condition of 274 patients with pancreatic cancer in our hospital from March 2012 to March 2017 were retrospectively analyzed (the follow-up was until June 2018 or the patient died) . Kaplan-Meier method was used to evaluate the survival of patients. Log-rank test was used for univariate analysis. Cox proportional hazard regression model was used for multivariate analysis to screen out the risk factors affecting the prognosis of patients with pancreatic cancer. Results By the end of follow-up, 242 cases died and 32 cases survived among 274 cases of patients, and the median survival time was 5.7 months, and the1-year, 2-year and 3-year survival rates were 24.6%, 10.7% and 4.6% respectively. Univariate analysis showed that tumor location, tumor size, c TNM stage, presence or absence of liver metastasis or lymph node metastasis, treatment methods, serum CEA level, serum CA199 level, serum CA242 level and NLR were associated with prognosis in patients with pancreatic cancer (P < 0.05) , but gender, age, BMI, presence or absence of diabetes history or smoking history, serum ALB level were not associated with prognosis in patients with pancreatic cancer (P > 0.05) . Cox multivariate analysis showed that tumor size > 2 cm, c TNM stage III-IV, lymph node metastasis, serum CA199 > 37 U/m L, serum CA242 > 20 U/m Ll and NLR > 4 were independent risk factors for prognosis of patients with pancreatic cancer (P<0.05) , and surgical treatment was a beneficial factor for prognosis of patients with pancreatic cancer (P < 0.05) . Conclusions Tumor size, c TNM stage, lymph node metastasis, serum CA199 and CA242 levels, NLR and treatment methods are closely related to the prognosis of patients with pancreatic cancer.
Objective To analyze the clinicopathological features of pancreatic cancer and the risk factors affecting the prognosis. Methods The clinical data and follow-up condition of 274 patients with pancreatic cancer in our hospital from March 2012 to March 2017 were retrospectively analyzed (the follow-up was until June 2018 or the patient died) . Kaplan-Meier method was used to evaluate the survival of patients. Log-rank test was used for univariate analysis. Cox proportional hazard regression model was used for multivariate analysis to screen out the risk factors affecting the prognosis of patients with pancreatic cancer. Results By the end of follow-up, 242 cases died and 32 cases survived among 274 cases of patients, and the median survival time was 5.7 months, and the1-year, 2-year and 3-year survival rates were 24.6%, 10.7% and 4.6% respectively. Univariate analysis showed that tumor location, tumor size, c TNM stage, presence or absence of liver metastasis or lymph node metastasis, treatment methods, serum CEA level, serum CA199 level, serum CA242 level and NLR were associated with prognosis in patients with pancreatic cancer (P < 0.05) , but gender, age, BMI, presence or absence of diabetes history or smoking history, serum ALB level were not associated with prognosis in patients with pancreatic cancer (P > 0.05) . Cox multivariate analysis showed that tumor size > 2 cm, c TNM stage III-IV, lymph node metastasis, serum CA199 > 37 U/m L, serum CA242 > 20 U/m Ll and NLR > 4 were independent risk factors for prognosis of patients with pancreatic cancer (P<0.05) , and surgical treatment was a beneficial factor for prognosis of patients with pancreatic cancer (P < 0.05) . Conclusions Tumor size, c TNM stage, lymph node metastasis, serum CA199 and CA242 levels, NLR and treatment methods are closely related to the prognosis of patients with pancreatic cancer.
2019, 40(03): 102-106.
Abstract:
Objective To understand the occurrence and development of hearing damage of enterprise workers in Liuzhou, and to provide technical basis for precaution of occupational disease. Methods The data of binaural high-frequency average hearing threshold from occupation health examination in Liuzhou during January 2015 and October 2017 were collected. The noise inspection results were analyzed and evaluated from 5 dimensions of district, enterprise scale, economy type, workers' age and gender. Results Totally 13465 workers received the noise special inspection in Liuzhou, January 2015 to October 2017, and the abnormality percentage of binaural high-frequency average hearing threshold result was 11.4%. The rate of abnormality was downwards, and the lowest rate of abnormality was in 2016 with 9.4% (P <0.001) . The abnormal rate of surrounding counties was high.Abnormal rate was higher in small and private township enterprises. Most of noise-exposed workers were male, and the abnormality percentage of binaural high-frequency average hearing threshold results was higher than that in the female. The abnormality percentage of binaural high-frequency average hearing threshold results was increasing accompany with the age growth, which was high in 40-49 year old group and 50-59 year old group. Conclusion The occupational health risk of middle-aged male noise-exposed workers in small-sized private township enterprises is serious in Liuzhou, and it is necessary to strengthen the noise control in these industries and population.
Objective To understand the occurrence and development of hearing damage of enterprise workers in Liuzhou, and to provide technical basis for precaution of occupational disease. Methods The data of binaural high-frequency average hearing threshold from occupation health examination in Liuzhou during January 2015 and October 2017 were collected. The noise inspection results were analyzed and evaluated from 5 dimensions of district, enterprise scale, economy type, workers' age and gender. Results Totally 13465 workers received the noise special inspection in Liuzhou, January 2015 to October 2017, and the abnormality percentage of binaural high-frequency average hearing threshold result was 11.4%. The rate of abnormality was downwards, and the lowest rate of abnormality was in 2016 with 9.4% (P <0.001) . The abnormal rate of surrounding counties was high.Abnormal rate was higher in small and private township enterprises. Most of noise-exposed workers were male, and the abnormality percentage of binaural high-frequency average hearing threshold results was higher than that in the female. The abnormality percentage of binaural high-frequency average hearing threshold results was increasing accompany with the age growth, which was high in 40-49 year old group and 50-59 year old group. Conclusion The occupational health risk of middle-aged male noise-exposed workers in small-sized private township enterprises is serious in Liuzhou, and it is necessary to strengthen the noise control in these industries and population.
2019, 40(03): 107-111.
Abstract:
Objective To study the application value of T-lymphocyte subsets for non-viral liver disease Methods180 cases of non-viral liver disease patients in The third people's Hospital of Kunming from June 1, 2017 to May 30, 2018 were collected.The peripheral blood of patients were tested and analyzed by BECKMAN Navios Flow Cytometer, and compared with of the reference ranges of peripheral blood T-lymphocyte subsets among healthy adults of Kunming.ROC analysis was used to test each index.Results Compared with the control group, CD3+, CD3+CD8+cells absolute number and the ratio of CD3+CD8+/lymphocytes with alcoholic cirrhosis group was significantly lower than the control group (P<0.05) , the ratio of CD4+/CD8+was significantly higher (P<0.001) .The ratio of CD3+CD4+/lymphocytes and CD4+/CD8+with primary biliary cirr Ilosis group was significantly higher than the control group (P<0.001) .The AUC value of CD3+, CD3+CD8+, CD3+CD8+/lymphocytes, CD4+/CD8+with alcoholic cirrhosis group was 0.80, 0.82, 0.66, 0.74.The AUC value of CD3+CD4+/lymphocytes and CD4+/CD8+with primary biliary cirr Ilosis group had a greater value, was 0.80, 0.81.ConclusionT lymphocyte subset can be the assistant diagnostic index of alcoholic cirrhosis and primary biliary cirrhosis.
Objective To study the application value of T-lymphocyte subsets for non-viral liver disease Methods180 cases of non-viral liver disease patients in The third people's Hospital of Kunming from June 1, 2017 to May 30, 2018 were collected.The peripheral blood of patients were tested and analyzed by BECKMAN Navios Flow Cytometer, and compared with of the reference ranges of peripheral blood T-lymphocyte subsets among healthy adults of Kunming.ROC analysis was used to test each index.Results Compared with the control group, CD3+, CD3+CD8+cells absolute number and the ratio of CD3+CD8+/lymphocytes with alcoholic cirrhosis group was significantly lower than the control group (P<0.05) , the ratio of CD4+/CD8+was significantly higher (P<0.001) .The ratio of CD3+CD4+/lymphocytes and CD4+/CD8+with primary biliary cirr Ilosis group was significantly higher than the control group (P<0.001) .The AUC value of CD3+, CD3+CD8+, CD3+CD8+/lymphocytes, CD4+/CD8+with alcoholic cirrhosis group was 0.80, 0.82, 0.66, 0.74.The AUC value of CD3+CD4+/lymphocytes and CD4+/CD8+with primary biliary cirr Ilosis group had a greater value, was 0.80, 0.81.ConclusionT lymphocyte subset can be the assistant diagnostic index of alcoholic cirrhosis and primary biliary cirrhosis.
2019, 40(03): 112-115.
Abstract:
Objective To analyze and compare rifampicin-resistant gene mutation detection and BACTEC MGIT 960 system phenotype susceptibility test in Mycobacterium tuberculosis by PCR-reverse dot blot hybridization.Methods At the same time, the phenotypic susceptibility of rifampicin to rifampicin was detected by PCR-reverse dot blot hybridization in sputum or bronchoalveolar lavage fluid (BALF) from 220 inpatients and by BACTEC MGIT 960 System. Results The susceptibility of rifampicin to rifampicin by the two methods accorded with 205 cases and the specificity was 93% (205/220) ; both methods were sensitive to rifampicin in 172 cases; gene resistance and phenotypic resistance in 33 cases, of which 3 cases detected mutation at the H526 locus of rpo B gene, with a mutation rate of 9%; gene resistance and phenotypic sensitivity in 15 cases, 11 of which were all detected. The mutation of the H526 locus of rpo B gene was 73%.Conclusions PCR-reverse dot hybridization has the characteristics of high speed, high efficiency and specificity. It has guiding significance for clinical diagnosis and treatment.
Objective To analyze and compare rifampicin-resistant gene mutation detection and BACTEC MGIT 960 system phenotype susceptibility test in Mycobacterium tuberculosis by PCR-reverse dot blot hybridization.Methods At the same time, the phenotypic susceptibility of rifampicin to rifampicin was detected by PCR-reverse dot blot hybridization in sputum or bronchoalveolar lavage fluid (BALF) from 220 inpatients and by BACTEC MGIT 960 System. Results The susceptibility of rifampicin to rifampicin by the two methods accorded with 205 cases and the specificity was 93% (205/220) ; both methods were sensitive to rifampicin in 172 cases; gene resistance and phenotypic resistance in 33 cases, of which 3 cases detected mutation at the H526 locus of rpo B gene, with a mutation rate of 9%; gene resistance and phenotypic sensitivity in 15 cases, 11 of which were all detected. The mutation of the H526 locus of rpo B gene was 73%.Conclusions PCR-reverse dot hybridization has the characteristics of high speed, high efficiency and specificity. It has guiding significance for clinical diagnosis and treatment.
2019, 40(03): 116-119.
Abstract:
Objective To explore the application value of ultrasound technology in clinical evaluation of right ventricular systolic function in children with funnel chest through using echocardiography and two-dimensional spot tracking, haller index (HI) of tricuspid ring displacement and CT examination to follow up patients before and six months after operation. Methods From March 2017 to August 2018, we selected 48 cases of children who were diagnosed with funnel chest and operated in our hospital from March 2017 to August 2018.Echocardiography measured M type (RVD) , right ventricular size M type tricuspid ring (TAPSE) , tricuspid ring diameter (TVAD) , the application of two-dimensional speckle tracking technology measure the tricuspid valve attachment points (TMAD1) , tricuspid valve attachment points before (TMAD2) , tricuspid ring attachment midpoint displacement (TMADm) and right ventricular systolic peak longitudinal shortening fraction (TMAD %) . Chest CT measures HI. Results There were statistically significant differences in RVD, TAPSE, TVAD and HI between preoperative and postoperative six months (P < 0.001) .The indexes were increased six months after operation. There was a statistical difference in TMAD% (P < 0.05) .TMAD1, TMAD2, and TMADm increased in the six months after operation, but there was no statistical difference (P > 0.05) . Conclusions Conventional echocardiography combined with two-dimensional speckle tracking technique is a reliable method to evaluate the cardiac structure and function before and after funnel chest surgery. It can provide objective basis for the cardiac compression of funnel chest and the damage of right ventricular systolic function. It can be used in the follow-up process of children with funnel chest.
Objective To explore the application value of ultrasound technology in clinical evaluation of right ventricular systolic function in children with funnel chest through using echocardiography and two-dimensional spot tracking, haller index (HI) of tricuspid ring displacement and CT examination to follow up patients before and six months after operation. Methods From March 2017 to August 2018, we selected 48 cases of children who were diagnosed with funnel chest and operated in our hospital from March 2017 to August 2018.Echocardiography measured M type (RVD) , right ventricular size M type tricuspid ring (TAPSE) , tricuspid ring diameter (TVAD) , the application of two-dimensional speckle tracking technology measure the tricuspid valve attachment points (TMAD1) , tricuspid valve attachment points before (TMAD2) , tricuspid ring attachment midpoint displacement (TMADm) and right ventricular systolic peak longitudinal shortening fraction (TMAD %) . Chest CT measures HI. Results There were statistically significant differences in RVD, TAPSE, TVAD and HI between preoperative and postoperative six months (P < 0.001) .The indexes were increased six months after operation. There was a statistical difference in TMAD% (P < 0.05) .TMAD1, TMAD2, and TMADm increased in the six months after operation, but there was no statistical difference (P > 0.05) . Conclusions Conventional echocardiography combined with two-dimensional speckle tracking technique is a reliable method to evaluate the cardiac structure and function before and after funnel chest surgery. It can provide objective basis for the cardiac compression of funnel chest and the damage of right ventricular systolic function. It can be used in the follow-up process of children with funnel chest.
2019, 40(03): 120-125.
Abstract:
Objective To study the genetic polymorphisms of 19 autosomal STR loci in 215 healthy unrelated individuals of Yunnan Yi population, calculate population genetic parameters, and establish genetic basis data of Yunnan Yi population, and provide scientific basis for forensic material identification and individual identification.Methods The sample DNA was extracted by Chelex-100 method, amplified by Power Plex 21 System kit, and the PCR composite amplification product was analyzed by ABI 3130 XL automatic genetic analyzer. STR was performed with ABI Gene Mapper v3.2 software. Genotyping analysis, statistical analysis of forensic genetic parameters and Hardy-Weinberg equilibrium test using Modified-Powerstates software. Results A total of 203 alleles and 666 genotypes were detected. Except D1 S1656, D5 S818, D12 S391, the genotype distribution accorded with Hardy-Weinberg equilibrium law (P > 0.05) , the cumulative non-parent exclusion rate (CPE) was 0.999 999 907, and the cumulative personal recognition ability (TDP) was 0.999 999 999 999 999. 999 9999. Conclusion The 19 autosomal loci have high polymorphism and good individual recognition ability in Yunnan Yi population, and can provide scientific genetic basic data for forensic individual identification and paternity identification.
Objective To study the genetic polymorphisms of 19 autosomal STR loci in 215 healthy unrelated individuals of Yunnan Yi population, calculate population genetic parameters, and establish genetic basis data of Yunnan Yi population, and provide scientific basis for forensic material identification and individual identification.Methods The sample DNA was extracted by Chelex-100 method, amplified by Power Plex 21 System kit, and the PCR composite amplification product was analyzed by ABI 3130 XL automatic genetic analyzer. STR was performed with ABI Gene Mapper v3.2 software. Genotyping analysis, statistical analysis of forensic genetic parameters and Hardy-Weinberg equilibrium test using Modified-Powerstates software. Results A total of 203 alleles and 666 genotypes were detected. Except D1 S1656, D5 S818, D12 S391, the genotype distribution accorded with Hardy-Weinberg equilibrium law (P > 0.05) , the cumulative non-parent exclusion rate (CPE) was 0.999 999 907, and the cumulative personal recognition ability (TDP) was 0.999 999 999 999 999. 999 9999. Conclusion The 19 autosomal loci have high polymorphism and good individual recognition ability in Yunnan Yi population, and can provide scientific genetic basic data for forensic individual identification and paternity identification.
2019, 40(03): 126-130.
Abstract:
As a new type of broad-spectrum insecticides, pyrethroid pesticides have been widely used in daily life and agriculture in recent years. Pyrethroid pesticides have attracted public concerns due to their increasing use and potential effects on human. On the basis of summarizing relevant researches, this paper reviews the effects of pyrethroid pesticides exposure on reproductive organs and hormone levels and thyroid hormone levels of human and growth of infants.
As a new type of broad-spectrum insecticides, pyrethroid pesticides have been widely used in daily life and agriculture in recent years. Pyrethroid pesticides have attracted public concerns due to their increasing use and potential effects on human. On the basis of summarizing relevant researches, this paper reviews the effects of pyrethroid pesticides exposure on reproductive organs and hormone levels and thyroid hormone levels of human and growth of infants.
2019, 40(03): 131-135.
Abstract:
Hyperuricemia is a foundation of multiple diseases, and lowering serum uric acid is able to effectively prevent and treat the diseases such as gout, renal dysfunction and cardiovascular diseases. Recently, new achievements in study on hyperuricemia treatment and prevention were emerged, which needs to be summed up.At first, the present article briefly reviewed the synthesis and elimination of uric acid, and grouped the ways of prevention and treatment into three strategies: inhibiting synthesis, enhancing excretion, and promoting degradation. In the end, the article put forward that future strategy should focus on intestinal tract to lower serum uric acid by local rather than systematic administration.
Hyperuricemia is a foundation of multiple diseases, and lowering serum uric acid is able to effectively prevent and treat the diseases such as gout, renal dysfunction and cardiovascular diseases. Recently, new achievements in study on hyperuricemia treatment and prevention were emerged, which needs to be summed up.At first, the present article briefly reviewed the synthesis and elimination of uric acid, and grouped the ways of prevention and treatment into three strategies: inhibiting synthesis, enhancing excretion, and promoting degradation. In the end, the article put forward that future strategy should focus on intestinal tract to lower serum uric acid by local rather than systematic administration.
2019, 40(03): 136-139.
Abstract:
Osteoarthritis (OA) is a common chronic degenerative disease affecting synovial joints (knee, hip, distal finger, spinal joint) . Lesions often affect the entire joint tissue, with the degradation of articular cartilage, joint bone Ankle formation and synovial lesions are characteristic. Most of the previous research and treatment focuses on articular cartilage lesions, more and more studies have shown that inflammatory lesions of the synovial membrane play an important role in the whole course of osteoarthritis, synovial inflammation and induction of inflammatory factors associated with synovium. The degradation and destruction of articular cartilage promotes the development of osteoarthritis. Synovial lesions play an important role in the development of osteoarthritis. Therefore, synovial inflammation can be a key target for the treatment of osteoarthritis.
Osteoarthritis (OA) is a common chronic degenerative disease affecting synovial joints (knee, hip, distal finger, spinal joint) . Lesions often affect the entire joint tissue, with the degradation of articular cartilage, joint bone Ankle formation and synovial lesions are characteristic. Most of the previous research and treatment focuses on articular cartilage lesions, more and more studies have shown that inflammatory lesions of the synovial membrane play an important role in the whole course of osteoarthritis, synovial inflammation and induction of inflammatory factors associated with synovium. The degradation and destruction of articular cartilage promotes the development of osteoarthritis. Synovial lesions play an important role in the development of osteoarthritis. Therefore, synovial inflammation can be a key target for the treatment of osteoarthritis.
2019, 40(03): 145-148.
Abstract:
Objective To study the application effect of rapid rehabilitation nursing in patients after uniport video-assisted thoracoscopic complete resection of lung cancer.Methods The experimental group adopted the concept of rapid rehabilitation surgery to provide care to 550 patients between June 2016 and December 2017. The control group retrospectively analyzed the nursing methods of 500 patients between September 2014 and May 2016.Two groups of patients underwent single operation with total thoracoscopic lung cancer complete resection. Results All patients underwent operations successfully and there were no mortality occurred during the postoperative period.For the experimental group, the first time to get out of bed, average length of stay after surgery, average tube extraction time and first exhaust time were shorter than those of the control group (P < 0.05) . The incidence of postoperative complications was significantly lower than that of the control group. 95.1% of the patients in the experimental group were very satisfied, significantly higher than the control group 91.0% (P < 0.05) .Conclusion The rapid rehabilitation nursing measures for patients after completely uniport thoracoscopic lobectomy can shorten the average length of hospital stay, shorten the time of the first bed activity, alleviate the pain and improve the[A1] nursing satisfaction.
Objective To study the application effect of rapid rehabilitation nursing in patients after uniport video-assisted thoracoscopic complete resection of lung cancer.Methods The experimental group adopted the concept of rapid rehabilitation surgery to provide care to 550 patients between June 2016 and December 2017. The control group retrospectively analyzed the nursing methods of 500 patients between September 2014 and May 2016.Two groups of patients underwent single operation with total thoracoscopic lung cancer complete resection. Results All patients underwent operations successfully and there were no mortality occurred during the postoperative period.For the experimental group, the first time to get out of bed, average length of stay after surgery, average tube extraction time and first exhaust time were shorter than those of the control group (P < 0.05) . The incidence of postoperative complications was significantly lower than that of the control group. 95.1% of the patients in the experimental group were very satisfied, significantly higher than the control group 91.0% (P < 0.05) .Conclusion The rapid rehabilitation nursing measures for patients after completely uniport thoracoscopic lobectomy can shorten the average length of hospital stay, shorten the time of the first bed activity, alleviate the pain and improve the[A1] nursing satisfaction.
