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RSS2021 Vol. 42, No. 4
2021, 42(4): 28-32.
doi: 10.12259/j.issn.2095-610X.S20210405
Abstract:
Objective To investigate brain abnormalities in rats with acute epilepsy by diffusional kurtosis imaging （DKI）. Methods MRI were performed on all rats. DKI images were obtained from 13 epilepsy rats and 9 normal controls. Fractional anisotropy （FA）, mean diffusivity （MD）, and mean kurtosis （MK）, axial kurtosis（K∥）, radial kurtosis（K⊥） of bilateral hippocampus, piriform cortex and entorhinal cortex were measured. SPSS 17.0 was used for statistical analysis. Results Compared with the control group, MD of bilateral piriform cortex/entorhinal cortex decreased, MK、K∥、K⊥ significantly increased in the model group （P < 0.05）. There were no statistically significant differences in FA, MD, MK, K∥ and K⊥ in the bilateral hippocampus of model group. Conclusion Using DKI to reveal the pathologic changes of rats with acute epilepsy provide theoretical basis for the pathophysiological changes of brain tissues of rats with acute epilepsy.
2021, 42(4): 1-7.
doi: 10.12259/j.issn.2095-610X.S20210401
Abstract:
Objective To explore the effect and mechanism of Puerarin on experimental autoimmune encephalomyelitis （EAE） in mice. Methods C57BL / 6 mice were randomly divided into control group, EAE group and purearin treated EAE group. From the first day of EAE induction, puerarin group was gavaged with Puerarin （80 mg/kg）, and the other two groups were given the same volume of normal saline once a day. The neurological function score and weight changes were recorded every day; the demyelination of spinal cord was detected by LFB myelination staining; the levels of IL-6, IL-10, IL-17, IL-22 and TGF-β in serum were detected by ELISA; the protein expression of NF-κB, IκBα, IL-17, Foxp3 in spinal cord tissue were detected by Western blot. Results Compared with EAE group, the neurological function score and average score of purearin group were significantly decreased （P < 0.01）, while the body weight was significantly increased （P < 0.01）. The demyelinating area （P < 0.01） and pathological score （P < 0.05） of EAE mice were significantly decreased after puerarin treatment. The levels of proinflammatory cytokines IL-6, IL-17 and IL-22 were significantly decreased in puerarin group （P < 0.01）, while IL-10 and TGF-β were significantly increased （P < 0.05）. After puerarin treatment, the expressions of NF-κB and IL-17 significantly decreased （P < 0.05）, and the expressions of IκBα and Foxp3 significantly increased （P < 0.01）. Conclusion Puerarin can alleviate the symptoms of EAE mice and reduce demyelination. The mechanism of action is to inhibit NF-κB pathway, to balance of IL-17/Tregs, and to regulate of immune function.
2021, 42(4): 8-12.
doi: 10.12259/j.issn.2095-610X.S20210402
Abstract:
Objective To study the effect of Gentiana rigescens Franch on the protein expression of NF - κ B and CTGF in lung tissue of mice with pulmonary fibrosis. Methods The model of pulmonary fibrosis in Kunming mice was established by intratracheal instillation of bleomycin （5 mg·kg-1）. Low, medium and high doses （50, 100, 200 mg·kg-1） of Gentiana rigescens Franch were administered intragastrically once a day. Pirfenidone was used as positive control. The mice were killed on the 28th day after modeling, and the lung tissues were taken for detection. Gross pathological changes of lung tissue were observed by naked eyes. Masson staining was used to observe the degree of pulmonary fibrosis. Western blot was used to detect the expression of NF - κ B and CTGF in lung tissue. Results Compared with the model group, the collagen deposition in lung tissue of mice was significantly reduced in different doses of Gentiana rigescens group and positive drug Pirfenidone group. Compared with the model group, the protein expression of NF - κ B and CTGF in lung tissue of mice were significantly down-regulated in different doses of Gentiana rigescens group and positive drug bifidone group （P < 0.05）. Conclusion Gentiana rigescens Franch has anti-pulmonary fibrotic effect, the anti-fibrotic mechanism is related to inhibiting lung inflammation and collagen deposition througth down-regulating the protein expression of NF - κ B and CTGF.
2021, 42(4): 13-19.
doi: 10.12259/j.issn.2095-610X.S20210403
Abstract:
Objective To study the relationship between different metabolites, metabolic pathways and toxicity in scopolamine intoxicated rats. Methods 40 Sprague-Dawley （SD） rats were divided into control group and 3 groups with different doses of drugs. Chronic intoxication model was established after continuous administration for 30 days. By using Gas chromatography-mass spectrometry （GC/MSD）, and multivariate analysis and database retrieval, endogenous metabolites in serum and urine of different groups of rats were analyzed to screen and identify potential differential metabolites. Metabolic pathway enrichment was carried out by MetaboAnalyst software. The paraffin sections were stained with HE and observed microscopically. Results The metabolic profile of the control group and the administration group was significantly different （P < 0.05）. In the serum and urine samples of the drug administration groups, 17 different metabolites, including glutamic acid, glycine, proline, creatine and alanine, were screened out, mainly involving 9 metabolic pathways, including alanine-aspartate-glutamic acid metabolism, glycine-serine-threonine metabolism, and glutamine-glutamic acid metabolism, etc. The main pathological result was neurodegeneration. Conclusion The toxic effect of scopolamine is mainly neurotoxicity, and its mechanism of toxicity may be related to its disturbance of alanine-aspartic acid-glutamic acid metabolism, glycine-serine-threonine metabolism, glutamine-glutamic acid metabolism.
2021, 42(4): 20-27.
doi: 10.12259/j.issn.2095-610X.S20210404
Abstract:
Objective To explore the protective effect and mechanisms of aqueous extract of Moringa oleifera leaves （EMO） on olanzapine （OLA） induced metabolic disorders in mice. Method 90 female mice were randomly divided into control group, OLA group （3 mg/kg）, positive control drug group （OLA + metformin （MET）, 3 mg/kg + 75 mg/kg）, EMO group （EMO-H/M/L. 400/200/100 mg/kg）, OLA + EMO group （OLA + EMO-H/M/L, 3 mg/kg + 400/200/100）. Each group was given intragastric administration for 14 days, and weight gain, food and water intake, fasting blood glucose, blood lipid, serum leptin, ghrelin, malondialdehyde （MDA）, serum peroxidase dismutase （SOD） activity, glutathione peroxidase （GSH-Px） activity, liver fatty acid synthase （FAS） and adipose differentiation related protein （ADRP） gene mRNA expression, liver tissue pathology damage were detected. Result Compared with control group, in OLA group food intake increased significantly （P < 0.01）, the contents of FBG, THC, TG and LDL-C, the levels of serum leptin and ghrelin increased significantly （P < 0.05 or P < 0.01）, and the expression of liver FAS and ADRP genes was significantly increased （P < 0.01）. Serum SOD and GSH-Px enzyme activities were significantly reduced （P < 0.05）, MDA content was significantly increased （P < 0.05）, and liver tissue damage was severe. Compared with the OLA group, in OLA + EMO-H / M group food and water intake significantly reduced （P < 0.05 or P < 0.01）, body weight gain significantly reduced （P < 0.05 or P < 0.01）, serum FBG and THC, TG, and LDL-C levels were significantly reduced （P < 0.05）, leptin and ghrelin levels were significantly reduced （P < 0.05）. Liver FAS and ADRP gene mRNA expression was significantly down-regulated, serum SOD and GSH-Px enzyme activities were significantly increased （P < 0.05）, MDA concentration was significantly reduced （P < 0.05）, and liver tissue damage was partially reversed. Compared the Control group, in the EMO group the above indicators were not significantly different. Conclusion EMO may partially reverse the glucose and lipid metabolism disorder induced by olanzapine. The mechanism is that the antioxidant effect protects the normal functions of tissues and organs, reduces the secretion of leptin and ghrelin, and reduces the symptoms of overeating. In addition, it can protect the cellular function of liver tissue, down-regulate the expression of genes related to fatty acid synthesis and transport, and reduce the content of blood lipid.
2021, 42(4): 33-37.
doi: 10.12259/j.issn.2095-610X.S20210406
Abstract:
Objective To compare prevalence of diabetes and its influencing factors between Han and Bai ethnic groups in rural Yunnan. Methods A total of 5, 003 rural residents aged ≥ 35 years in Fumin County of Kunming city and Jianchuan County of Dali Bai Autonomous Prefecture were selected by multi-stage stratified random sampling method, each participant received on-site questionnaire interview and physical examination. Chi-square test was used to analyze the difference of diabetes prevalence between Han and Bai ethnic groups, and multivariate logistic regression was used to analyze the influencing factors of diabetes prevalence. Results The prevalence of prediabetes in Han and Bai was 16.30% and 14.43%, and the prevalence of diabetes was 11.83% and 7.83%, respectively. The prevalence of diabetes in Han was higher than that in Bai by gender, educational level and per capita annual income （P < 0.05）. Multivariate logistic regression analysis results indicated that older age （OR = 1.016 and OR = 1.032）, obese （OR = 1.580 and OR = 2.561）, central obese （OR = 2.372 and OR = 1.593）, physical inactivity （OR = 1.246 and OR = 1.312） and family history of diabetes （OR = 3.925 and OR = 3.781） are risk factors of diabetes in both Han and Bai ethnic population （P < 0.05）. In addition, Han people with higher per capita annual income were more likely to develop diabetes （OR = 1.459, P < 0.05）, and males in Bai ethnic minority had higher risk of suffering from diabetes than females （OR = 0.561, P < 0.05）. Conclusion The prevalence of prediabetes and diabetes is different between Bai and Han ethnic groups; and older age, obesity, central obesity, lack of physical activity and family history of diabetes are risk factors for diabetes. Han and Bai people should build healthy habits and exercise regularly to reduce the risk of diabetes.
2021, 42(4): 38-43.
doi: 10.12259/j.issn.2095-610X.S20210407
Abstract:
Objective To investigate the periodontal status of rural elderly aged 65 to 74 in Ning’ er and Jianchuan counties of Yunnan Province and to analyze the risk factors of periodontal disease. Methods Gingival bleeding and loss of attachment were recorded according to World Health Organization standards and a face-to-face questionnaire was conducted using a multi-stage stratified sampling method. The prevalence and related factors were analyzed by SPSS 25.0 statistical software. Results In rural areas of Yunnan province, the prevalence of gingival bleeding was 86.6% and prevalence of loss of attachment > 3 mm was 75.7%. The average number of gingival bleeding teeth was 8.96（2, 14）. Periodontal health status was related to sex, nationality, residence, oral health knowledge, dental experience and frequency of brushing （P < 0.05）. Conclusion The prevalence of periodontal disease in rural elderly aged 65 to 74 years in Ning’ er and Jianchuan counties of Yunnan Province is high; prevention and treatment should be carried out on the basis of risk factors.
2021, 42(4): 44-48.
doi: 10.12259/j.issn.2095-610X.S20210408
Abstract:
Objective To investigate the serum fat-soluble vitamins levels in infants and children in Anning City, to provide reference data for reasonable fat-soluble vitamin supplementation. Methods From June 2019 to July 2020, 925 infants and children from Children’ s Physical Examination Center of Kungang Hospital were selected. High performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS） was used to test the serum fat-soluble vitamins quantitatively. Then the fat-soluble vitamins levels were analyzed based on different ages, genders, and seasons. Results The average levels as well as insufficiency and deficient rates of serum vitamin A, 25-hydroxyvitamin D3, vitamin E and vitamin K in 925 infants and children were （284±3.84） ng/mL, 61.08%（565/925）; （34.27±0.46）ng/mL, 1.73%（16/925）; （8.47±0.16）μg/mL, 12.11%（112/925） and （0.46±0.03）ng/mL, 19.68%（182/925）, respectively. There were differences between different age groups （age range 0-3, 4-6, 7-12） and seasons （P < 0.01）. Conclusion There was a serious deficiency of vitamin A, which requires close attention and reasonable supplement. Due to the natural sunshine advantage in Yunnan, vitamin D nutrition level is relatively good, and excessive supplementation should be avoided.
2021, 42(4): 53-56.
doi: 10.12259/j.issn.2095-610X.S20210410
Abstract:
Objective To analyze the clinical efficacy and safety of valsartan amlodipine tablets combined with alpha-lipoic acid in the treatment of elderly type 2 diabetic nephropathy complicated with hypertension. Methods A total of 40 elderly patients with type 2 diabetic nephropathy complicated with hypertension who were admitted to the First Affiliated Hospital of China Medical University from February 2018 to August 2019 were enrolled in this study. They were divided into control group and observation group by pairing method. In the control group （n = 20）, there were 13 males and 7 females with an average age of （64.24±1.16）. In the observation group （n = 20）, there were 11 males and 9 females with an average age of （65.12±1.12）. Patients in both groups received routine hypoglycemic therapy, the control group was treated with valsartan and amlodipine tablets, and the observation group was treated with valsartan and amlodipine tablets combined with α-lipoic acid. Diastolic blood pressure （DBP）, systolic blood pressure （SBP）, Urinary albumin excretion rate （UAER）, blood nitrogen （BUN）, serum creatinine （SCR） and adverse reactions were compared between the observation group and the control group before and after treatment. Results The SBP, DBP, UAER and adverse reactions in the observation group were lower than those in the control group, and the efficacy and safety of the treatment were better than those in the control group with single drug （valsartan amlodipine tablet）, the difference was statistically significant （P < 0.05）. Conclusion Compared with valsartan amlodipine tablets alone, valsartan amlodipine tablets have better therapeutic effect, more stable, better safety and better patient satisfaction degree after two months of treatment, which is worthy of to long-term clinical application and promotion.
2021, 42(4): 96-101.
doi: 10.12259/j.issn.2095-610X.S20210418
Abstract:
Objective To compare windowing the femur diaphysis and extended trochanteric osteotomy for hip implants extraction in revision surgery. Methods We retrospective analyzed data of 18 patients （18 hips） with primary hip arthroplasty undergone windowing the femur diaphysis for implants removal from January 2014 to March 2019. Twelve patients （12 hips） treated with ETO for implants removal associated with femur revision surgery at the same stage were chosen as control group. Operation time and blood loss were recorded, and Harris score and Abductor MRC score were used to evaluate the hip function. Radiographic follow-up was performed to observe the position of the prosthesis and the healing of the osteotomy, and to evaluate and record the presence of complications related to steel wires. Results There was no statistically difference in operation time and blood loss between the two groups （P > 0.05）. The Harris score and abductor muscle strength （MRC） score in the windowing group were higher than those in the ETO group, and the differences were statistically significant （ P < 0.05）. During the follow-up, there were 2 cases of subsidence in the window-opening group, which did not affect the stability of the prosthesis, and no further subsidence was observed after 6 months of follow-up; while no prosthesis subsidence and osteotomy displacement were observed in ETO group. After 1 year of follow-up, all patients were able to walk with weight, and the X-ray films showed that the osteotomy was healed in both groups. No complications related to fixation using a cable or wire cable was seen during short-term follow-up. Conclusion Compared with ETO, the proximal of the femur with the muscle insertions remain intact in windowing technique, so patient can start exercise earlier after operation. And cementless stem can be used for revision surgery. During short-term follow-up, the hip functions in windowing group are better than those in ETO group. The osteotomized fragment in window technique is smaller than ETO, so it can be fixed without cable wire or titanium band and related complications avoided.
2021, 42(4): 122-127.
doi: 10.12259/j.issn.2095-610X.S20210423
Abstract:
Objective To investigate the clinical efficacy of cerebrospinal fluid drainage and injection by Ommaya reservoir implantation and lumbar cistern intervention in the treatment of patients of tuberculous meningoencephalitis with hydrocephalus. Methods A total of 50 patients with tuberculous meningoencephalitis with hydrocephalus in our hospital from March 2017 to September 2018 participated in this study. Participants were divided into 3 groups: control group, Ommaya reservoir group and lumbar cistern intervention group. The control group received routine antituberculosis; Ommaya reservoir group received routine antituberculous therapy combined with cerebrospinal fluid drainage and lateral ventricle injection; and lumbar cistern intervention group received anti-tuberculosis treatment combined with lumbar cistern cerebrospinal fluid drainage and intrathecal injection. The treatment duration was 4 weeks. The efficacy and adverse reactions of the three groups were compared and analyzed. Results The clinical symptom remission time of Ommaya reservoir group and lumbar cistern intervention group was shorter than that of control group （P < 0.05）. The intracranial pressure at 3 days, 1 week, 2 weeks and 4 weeks after treatment was significantly different from that before treatment （ P < 0.05）. Compared with the control group, there were statistically significant differences in the cerebrospinal fluid biochemical indexes in the lumbar cistern intervention group and the Ommaya reservoir group after 1 week of treatment （ P < 0.05）. 2 months after treatment, the absorption of intracranial lesions in the Ommaya reservoir group was significantly higher than that in the other two groups （ P < 0.05）. The total effective rates of the control group, the lumbar cistern intervention group and the Ommaya reservoir group were 45%, 83.3% and 100%, respectively （ P < 0.05）. Conclusion The interventional treatment of tuberculous meningoencephalitis with hydrocephalus by lumbar cistern catheterization and Ommaya reservoir implant can relieve the clinical symptoms, reduce intracranial pressure and improve the biochemical indexes of cerebrospinal fluid in a short time. Interventional therapy via the Ommaya reservoir approach promotes the absorption of intracranial lesions more effectively than interventional therapy via the lumbar cistern.
2021, 42(4): 128-133.
doi: 10.12259/j.issn.2095-610X.S20210424
Abstract:
Objective To analyse the diagnostic advantages and therapeutic value of 3D-DSA compared with conventional 2D-DSA in nerointervention in the “Gold Standard”. Methods A total of 277 cases who underwent neurointerventional therapy using 2D and 3D-DSA were collected from the Affiliated Yan’ an Hospital of Kunming Medical University from January 2018 to December 2019, including 111 cases of cerebral thrombectomy, 106 cases/137 aneurysms （97 common cystic aneurysms in 75 cases, 6 giant aneurysms in 5 cases, 29 wide neck aneurysms in 21 cases, 2 dissecting aneurysms in 2 cases, 2 spindle aneurysms in 2 cases, 1 complex aneurysm in 1 case）, 45 cases of arteriovenous malformation and 15 cases of carotid artery stenosis stent implantation. The 2D and 3D images were retrospectively analyzed and measured by two experienced radiologists using double-blind method, and then the difference of the measured data and the detection rate of the disease were compared. Results There was no significant difference between 2D-DSA and 3D-DSA in the detection rate of each segment of cerebral thrombosis （P > 0.05）. 122 aneurysms （93 cases） were detected by 2D-DSA, 137 aneurysms （106 cases） were detected by 3D-DSA （ P < 0.05）, excepted 2 false positive cases. 44 cases of AVM were detected by 2D-DSA and 45 cases were detected by 3D-DSA （ P > 0.05）. 2D-DSA showed carotid artery stenosis in 15 cases and 3D-DSA showed carotid artery stenosis in 15 cases, the difference was not statistically significant （ P > 0.05）. 3D-DSA is significantly better than 2D-DSA in the detection rate of intracranial aneurysm, tumor neck, tumor shape, tumor diameter, tumor tumor-bearing vessels, perforator vessel, etc. Conclusion 3D-DSA technology improves the detection rate of neurological diseases, improves the accuracy of qualitative and quantitative diagnosis of neurological diseases; therefore it has important guiding significance for the further treatment of diseases.
2021, 42(4): 138-141.
doi: 10.12259/j.issn.2095-610X.S20210426
Abstract:
Objective To explore the pathogenic composition of hand-foot-and-mouth disease （HFMD） in outpatient of The Maternal and Child Health Hospital of Kunming from 2014 to 2018, so as to understand the immune protection status of enterovirus 71 （EV71） vaccine, and to provide clinical basis for EV71 vaccination promotion of enterovirus. Methods Fecal samples of children diagnosed with hand-foot-and-mouth disease in outpatient and emergency department of The Maternal and Child Health Hospital of Kunming from 2014 to 2018 were collected for detection of EV71, coxsackievirus A 16 and enterovirus universal by PCR. We divided these HFMD children into observation group and control group, according to vaccination of EV71, and analysed EV71 and non-EV71 pathogens ratio of two groups. Multivariate analysis was performed with EV71 infection status, vaccination, gender and age as independent variables. Results The constituent ratio of EV71 in the observation group and the control group were 0.6% and 15%, respectively, and there were statistically significant difference （χ2 = 27.088, P < 0.05）. Multi-factor analysis showed that vaccination status was influencing factor; vaccination was protective factor with protection rate of 96.8%. Conclusion Among the children with HFMD in the outpatient and emergency department of Kunming Maternal and Child Health Hospital, the infection rate of EV71 was lower in those who had been vaccinated with EV71 inactivated vaccine than those who had not.Therefore, the promotion of vaccine vaccination also had a good effect on preventing hand-foot-mouth disease caused by EV71 infection in the population.
2021, 42(4): 142-145.
doi: 10.12259/j.issn.2095-610X.S20210427
Abstract:
Objective To study the efficacy of HIMSS stage 6 on the number and types of medication order errors. Methods Data of medication order errors after application of HIMSS stage 6 technology were collected as the study group, and data of medication order errors before application of HIMSS stage 6 technology were collected as the control group. The number and types of medication order errors were compared between the two groups. Results After the application of this technology, there were 265 borderline errors in medication orders, including 204 （76.98%） of usage and dosage, 56 （21.13%） of dosage form or route of administration, 5（1.89%） of repeated drug use, and 0 contraindication, which were significantly lower than those in control group （P < 0.05）. There were 71 borderline errors in pharmacists’ dispensing orders, including 48 cases of high warning drugs （67.60%）, 18（25.35%） in peak time of drug dispense, 5（7.04%） in adjacent placing, which were significantly lower than those in the control group （ P < 0.05）. Conclusion Using HIMSS stage 6 technology can reduce medication errors and ensure medication safety for children.
2021, 42(4): 49-52.
doi: 10.12259/j.issn.2095-610X.S20210409
Abstract:
Objective To investigate the expression of long non-coding RNA （IncRNA） APTR, HEIH, FAS-ASA1, FAM83H-AS1, DICER1-AS1 and PR-lncRNA in lung cancer patients. METHODS Total RNA was extracted by TRI Z O L from cancer tissues, adjacent normal tissues and lymph node metastases of lung cancer patients in the Third Affiliated Hospital of Kunming Medical University. The relative RNA expression of each gene was detected by SYBR GR e e N real-time quantitative fluorescence PCR. RESULTS The expression of APTR was increased in lymphoid tissues （P < 0.05）, the expression of HEIH was decreased in lymphoid tissues （P < 0.05）, and the expression of FAM83H-AS1 and PR-lncRNA were increased in lung cancer tissues （P < 0.05）. Conclusion Increased expression of APTR in lymphoid tissue may promote lymph node metastasis of lung cancer; increased expression of FAM83H-AS1 and PR-lncRNA in cancer tissue may promote the occurrence and development of lung cancer.
2021, 42(4): 57-60.
doi: 10.12259/j.issn.2095-610X.S20210411
Abstract:
Objective To investigate the abnormal structure of temporomandibular joint （TMJ） in patients before orthodontic treatment, and to provide guidance for clinical treatment. Methods A total of 175 patients aged 10-40 years before orthodontic treatment in our hospital from October 2015 to October 2019 were enrolled as study subjects. Based on age, the 175 patients were divided into three groups.Anteroposterior radiographs of the head and skull, oral panoramic radiographs, and bilateral temporomandibular joint open and closed position MRI were routinely taken; age, gender, classification of malocclusion and the incidence of temporomandibular joint structural abnormality were analyzed. Result Among the 175 patients, 114 cases had TMJ structural abnormality, and the abnormality rate was 65.14%. among 84 patients aged from 10 to 20 years, 71 were abnormal, the abnormal rate was 84.52%; among 68 patients aged from 21 to 30 years, 32 cases were abnormal, the abnormal rate was 47.06%.; among the 23 patients aged from 31 to 40 years, 11 were abnormal, with an abnormal rate of 47.83%. The abnormal rate of joint structure in 10-20-year group was higher than that in the other two groups, the difference was statistically significant （P < 0.05）. Analysing based on malocclusion deformity type, the abnormal rate of Angle II is 92.68%, Angle I is 37.25% and Angle III is 40.48%. The abnormal rate of Angle II is significantly higher than that of Angle I class and III class, the difference is statistically significant （P < 0.05）. The abnormal rate was 90.27% in female and 19.35% in male. The gender difference was statistically significant （P < 0.05）. Conclusion Temporomandibular joint disorder are very common among the patients seeking orthodontic treatment, and the clinical manifestations of such patients are often mild and tend to be ignored. Especially those 10-20-year-old Angle Ⅱ class deformity of female patients should raise more attention of orthodontic doctors.
2021, 42(4): 61-66.
doi: 10.12259/j.issn.2095-610X.S20210412
Abstract:
Objective To investigate the changes of upper and lower limb artery PaO2 and blood lactic acid in rectal cancer patients with different ventilation modes in Trendelenburg position during perioperative period, so as to provide basis and reference for the reasonable implementation of perioperative body function protection strategy. Methods Forty patients underwent elective laparoscopic radical resection of rectal cancer were randomly divided into two groups: volume control ventilation group （VCV group, 20 cases） and pressure control ventilation group （PCV group, 20 cases）. In each group, the changes of PaO2 and blood lactic acid in the ipsilateral upper and lower limbs of patients with rectal cancer at different time points were observed and compared under Trendelenburg position. Results The PaO2 of the upper limbs was higher than that of the lower limbs in the two groups. In the VCV group, there was statistically significant difference in PaO2 in the arteries of upper and lower limbs at T1 and T4 （P < 0.05）. In PCV group, there was statistically significant difference in PaO2 of upper and lower limb arteries at T0 and T4 （P < 0.05）. PaO2 in the upper and lower extremity arterial blood of PCV group was higher than that of VCV group, and the difference of PaO2 in the upper extremity artery at T2 and T3 between the two groups was statistically significant （P < 0.05）.The difference of lower limb artery PaO2 between the two groups at T1-4 was statistically significant （P < 0.05）. Compared with T0 time point, PaO2 in the upper extremity of VCV group was significantly decreased at T2-4 time point, and that in the lower extremity of VCV group was significantly decreased at T1-4 time point, the difference was statistically significant （P < 0.05）. In the PCV group, the upper and lower limbs arterial PaO2 decreased at T5, and the difference was statistically significant （P < 0.05）. The levels of arterial lactic acid in upper limbs were lower than those in lower limbs in 2 groups, and there was statistical significance in the values of arterial lactic acid in upper and lower limbs in VCV group at T2 and T4 （P < 0.05）. There was statistically significant difference in arterial lactic acid value of upper and lower limbs in PCV group at T3 （P < 0.05）. The arterial lactic acid in the upper and lower limbs of the PCV group was lower than that of the VCV group, and the arterial lactic acid in the lower limbs of the PCV group was significantly lower than that of the VCV group at T0, T2 and T3, with statistical significance （P < 0.05）. Conclusion PCV ventilation mode in perioperative period has more advantages in improving arterial blood PaO2, reducing blood lactic acid, improving tissue perfusion. The advantages of PCV were more prominent in the lower limbs which can reduce the adverse effect of Trendelenburg posture on patients.
2021, 42(4): 67-72.
doi: 10.12259/j.issn.2095-610X.S20210413
Abstract:
Objective To investigate the expression of new adaptor protein XB130 in hepatocellular carcinoma （HCC） tissues and adjacent tissues and its relationship with histopathology, and to explore its influence on the invasion and migration of hepatocellular carcinoma cells. Methods We collected and analyzed the pathological sections of stained HCC samples. The expression of XB130 in HCC cell lines HCCLM3, HepG2, SMMC-7721, Hep3B and MHCC-97 was screened and the XB130 gene was inhibited. The expression of XB130 was detected by Western blotting and RT-PCR. Cell invasion ability was observed using Tranwell and cell migration ability was observed through cell scratches. Results Among the 90 HCC samples, the positive staining rate was 44.4% （40 cases）; among the positive staining samples, 29 cases were strongly positive and 11 cases were moderately to weakly positive. The study also found that among these samples, 24 cases were patients with recurrence. The expression of XB130 in HCC tissues was significantly higher than that in adjacent tissues （P < 0.05）. The higher the expression of XB130 in HCC, the shorter the postoperative survival time and the higher recurrence rate of patients （P < 0.05）. The relative expression level, cell invasion and migration ability of XB130 were decreased after inhibiting XB130 gene （P < 0.05）. Conclusion XB130 is expected to become a new prognostic marker of HCC. Down-regulating the expression of XB130 in HCC cells can alleviate its invasion and migration.
2021, 42(4): 73-77.
doi: 10.12259/j.issn.2095-610X.S20210414
Abstract:
Objective To study the effect of Plasma Exchange （PE） combined with Double Plasma Molecular Absorption System （DPMAS） on cytokines in patients with autoimmune hepatitis complicated with liver failure, and to explore the significance of Plasma Exchange combined with Double Plasma Molecular absorption in immunotherapy and the impact on the prognosis of such patients. Methods In this study, 22 patients with autoimmune hepatitis complicated with liver failure who were hospitalized in the Third People’ s Hospital of Kunming City from October 2017 to August 2020 were selected. These patients received plasma exchange combined with double plasma molecular adsorption therapy, and the changes of cytokines （IL-2, IL-4, IL-6, IL-10, TNF-α）, liver function, blood ammonia, coagulation function, blood routine and other indicators before and after the combined medical treatment were analyzed. Results After treatment, the levels of cytokines IL-2, IL-6, and TNF-α, liver function （TBil, AST, ALT, ALB）, blood ammonia, prothrombin activity, hemoglobin, platelet decreased significantly, （P < 0.05）; while the differences in cytokines IL-4 and IL-10）, total bile acid and other indicators were statistically insignificant （P > 0.05）. Conclusion Selective plasma exchage combined with double plasma molecular adsorption in the treatment of patients with autoimmune hepatitis complicated with liver failure helps to maintain homeostasis and supports the regeneration of liver cells, so as to restore liver function and improve clinical symptoms such as jaundice. This method can improve the survival rate of patients, improve the success rate of liver failure treatment, and is worthy of clinical promotion.
2021, 42(4): 78-83.
doi: 10.12259/j.issn.2095-610X.S20210415
Abstract:
Objective To investigate the expression of costimulatory molecule B7-H4 in intrahepatic cholangiocarcinoma （ICC）and its clinical significance. Methods Immunohistochemistry was used to detect the expression of B7-H4 in 90 cases of intrahepatic cholangiocarcinoma and its adjacent tissues, and analyzed the correlation between B7-H4 expression and the clinicopathological characteristics and prognosis of the patients. Results The expression rate of B7-H4 in intrahepatic cholangiocarcinoma was 44.4%, which was significantly higher than that of adjacent tissues. The increased expression of B7-H4 in intrahepatic cholangiocarcinoma was related to TNM staging, tumor differentiation, lymph node metastasis and poor prognosis （P < 0.05）. Survival analysis revealed that the overall survival rate of patients in the B7-H4 high expression group was significantly lower than that in the B7-H4 low expression group （P = 0.005）. Cox proportional hazards regression model analysis showed that B7-H4 is an independent prognostic factor for patients with intrahepatic cholangiocarcinoma. Conclusion The expression of B7-H4 is increased in intrahepatic cholangiocarcinoma tissues, and the high expression of B7-H4 is closely related to the malignant pathological characteristics and poor prognosis of ICC patients, which has clinical guiding significance for predicting the prognosis of patients.
2021, 42(4): 84-90.
doi: 10.12259/j.issn.2095-610X.S20210416
Abstract:
Objective To explore the effects of different concentrations of magnesium ion intervention on endothelial progenitor cells （endothelial progenitor cells, EPCs） in women with gestational diabetes （gestaional diabetes mellitus, GDM）. Methods 204 cases of gestational diabetes mellitus from May 2016 to March 2020 were selected as the observation group, and 204 cases of normal pregnant women hospitalized during the same period were selected as the control group. The observation group was divided into group A （4 mmol/L）, group B （8 mmol/L） and group C （16 mmol/L） by random number table method, and were treated with different concentrations of magnesium ions. Using the same method, the control group was divided into group a （4 mmol/L）, group b （8 mmol/L） and group c （16 mmol/L）, and was also given different concentrations of magnesium ions. There were 68 patients in each of the six groups. In the six groups of patients, each pregnant woman was enrolled for cord blood isolation and culture, and each case was enrolled with 6-well cells. The cell proliferation, apoptosis, adhesion and cell cycle of EPCs were compared. Results The proliferative capacity of the three groups of women in the observation group was lower than the those of control group, with group B better than group A, group A better than group C, group b better than group a, and group a better than group c. The apoptosis rate is 100% in normal population. The proliferative capacity of the three groups of women in the observation group is higher than that in the control group, with group B lower than group A, group A lower than group C, group b lower than group a, group a lower than group c, the difference is statistically significant （P < 0.05）. According to the calculation using Image J software, the cell area and cell density in the observation group were lower than those in the control group, with group B higher than group A, group A higher than group C, group b higher than group a, and group a higher than group c; the difference is statistically significant （P < 0.05）. The occurrence of G0 / G1 phase cell cycle arrest in the observation group was lower than that in control group, with group B better than group A, group A better than group C, group b better than group a, and group a better than group c, the difference is statistically significant （P < 0.05）. Conclusion 8 mmol/L magnesium ion may be associated with the proliferation and adhesion of EPCs in pregnant women, thus inhibiting apoptosis and upregulating cell cycle, and its effect on women with GDM is more evident, which can be used to help improve the pregnancy outcome of pregnant women with GDM after further study.
2021, 42(4): 91-95.
doi: 10.12259/j.issn.2095-610X.S20210417
Abstract:
Objective To investigate the clinical characteristics of female patients with myocardial infarction and the differences in serum levels of endoplasmic reticulum stress proteins HSP47, XBP-1S, and GRP78 with male patients. Methods A total of 356 patients with acute myocardial infarction who were admitted to the hospital. The venous blood was collected within 24 hours after the onset, and the serum HSP47, XBP-1S and GRP78 concentrations were measured after centrifugation. They were divided into female group （n = 116） and male group （n = 240）. The independent sample t test, chi-square test and nonparametric rank sum test were used for clinical data analysis. Results The age of onset in the female group was older than that in the male group （P < 0.05）; while the prevalence of smoking and hypertension in the female group were lower than those in the male group （P < 0.05）. The level of XBP-1S in serum of female patients was higher than that of male patients （P = 0.023, P < 0.05）; but there was no significant difference in serum HSP47 and GRP78 levels between female patients and male patients （P > 0.05）. Serum GLU and BNP levels in the female patient group were higher than those in the male patient group （both P < 0.05）, and the female patient group had lower levels of HCY （mmol/L）, CREA （mmol/L）, uric acid （mmol/L） and eGFRm than in the male group （all P < 0.05）. The incidence of non-ST-segment elevation myocardial infarction was 47.4% higher than that of the male group （P < 0.05）. Conclusion Female patients with acute myocardial infarction have an older age of onset, higher serum GLU and BNP levels. The incidence of non-ST segment elevation myocardial infarction is higher in the female group and higher endoplasmic reticulum stress protein XBP-1S level within 24 hours of onset; while the serum levels of HCY, CREA, uric acid, and eGFRm in male patients with acute myocardial infarction were higher than those in the female group.
2021, 42(4): 102-106.
doi: 10.12259/j.issn.2095-610X.S20210419
Abstract:
Objective To study the efficacy of proximal gastrectomy with double tract reconstruction and total radical gastrectomy for upper gastric cancer. Methods 50 patients who underwent radical resection of upper gastric cancer in Suzhou Jiulong Hospital from April 2014 to April 2019 were included in the study and were randomly divided into two groups. Observation group （25 cases） underwent radical proximal gastrectomy and double-tract reconstruction, and control group （25 cases） underwent total radical gastrectomy. Intraoperative blood loss, operation time, postoperative anal exhaust time, postoperative feeding time, postoperative ambulance time and postoperative hospitalization were observed and recorded in the two groups. The incidence of postoperative complications, single food intake, body weight, the ratio of serum pepsin type Ⅰ to Ⅱ, serum gastrin, hemoglobin and plasma albumin were compared between the two groups before and 6 months after operation. Results The intraoperative blood loss, operation time, postoperative ambulation time and postoperative hospitalization in the observation group were significantly lower than those in the control group （P < 0.05）; there was no significant difference in the first anal exhaust time and postoperative eating time between the two groups （P > 0.05）. The incidence of adverse reactions in the two groups were 20.00% and 36.00%, respectively. There was no significant difference between the two groups （P > 0.05）. 6 months after operation, amount of food intake per meal, serum gastrin, serum pepsin typeⅠ/Ⅱ were significantly lower than those before operation （P < 0.05）. Six months after operation, the serum gastrin in observation group was significantly higher than that in control group （P < 0.05）, and there was no statistical significance in the amount of food intake per meal and the pepsin typeⅠ/Ⅱ of serum compared with control group （P > 0.05）. The hemoglobin, plasma albumin and body mass at 6 months after operation in the two groups were significantly lower than those before operation （P < 0.05）; the hemoglobin, plasma albumin and body mass of the observation group at 6 months after operation were significantly higher than those of the control group （P < 0.05）. Conclusion Double tract reconstruction of proximal gastrectomy preserved function of the stomach and duodenum, so the patient has a better long-term nutritional status. The operation procedure is relatively easy causing less injury to patients. Therefore, it is an ideal way to reconstruct digestive tract in the radical operation of upper gastric carcinoma.
2021, 42(4): 107-112.
doi: 10.12259/j.issn.2095-610X.S20210420
Abstract:
Objective To explore the clinical efficacy of the treatment of subrenal iliac abdominal artery disease with integrated bifurcated stent graft. Methods Retrospective analysis was performed on 87 cases of subrenal abdominal iliac artery disease treated by intracavitary isolation with unibody bifurcated stent in the Department of Vascular Interventional Surgery of Yan'an Hospital Affiliated to Kunming Medical University from August 2015 to October 2019. Results 87 patients were successfully implanted with unibody bifurcated stent, the success rate was 100%. Only one unibody bifurcated stent was implanted in 15 cases, one leg stent was inserted in 36 cases, two leg stents were inserted in 29 cases, three leg stents were inserted in 4 cases, and four leg stents were inserted in 3 cases. Due to the large angle and distorted course of the abdominal iliac artery, 5 cases were treated with a bare stent inserted into the iliac branch of the integrated bifurcated coated stent, and 3 cases were treated with a bare stent distally; One case received integrated bifurcated coated stent with straight tube CUFF coated stent above it, 14 cases underwent internal iliac artery embolization first, 13 cases retained bilateral internal iliac artery, 51 cases retained one internal iliac artery, 23 cases closed both sides of internal iliac artery. Balloon was used to expand both ends and joints of the stent after surgery. The operation time was 35 min-122 min, with an average of 52 min, blood loss during operation was 10-50 ml, with an average of 23 ml, and 87 cases successfully completed the operation. One case developed renal failure and multiple organ failure after operation, 85 cases were followed up for 2-24 months, all of them were alive, 1 case was lost. CTA was reexamined 3 to 6 months after operation: the stent was in good shape and position, without internal leakage and displacement, and the lesions were closed well. Conclusions The use of the integrated bifurcated stent graft endovascular isolation for the treatment of subrenal abdominal iliac artery disease has many advantages, such as minimal invasion, good efffect, easy operation, high success rate, short operation time, safety, less complications, quick recovery and so on. The short-term and medium-term effect is better, the long-term effect remains to be observed.
2021, 42(4): 113-116.
doi: 10.12259/j.issn.2095-610X.S20210421
Abstract:
Objective To evaluate the application value of vectorcardiogram （VCG） in the diagnosis of left ventricular abnormalities in the early stage of hypertension. Methods The electrocardiogram （ECG）, VCG and ultrasonic cardiogram （UCG） of 76 patients with essential hypertension at the same visit or during same hospitalization period were retrospectively analyzed. The initial vector and spatial maximum vector of VCG were used as the indicators of left ventricular abnormality, as well as the interventricular septum, left ventricular posterior wall and left ventricular end-diastolic diameter of UCG. The positive detection of the two were compared; and the detection of VCG and ECG in abnormal left ventricular electrical activity was compared. Results The detection rate of VCG in left ventricular abnormality was 55.3%, which was significantly higher than that of UCG （38.2%, P < 0.05）.The positive rate of VCG in abnormal left ventricular electrical activity was 88.2%, which was significantly higher than that of ECG （71.1%, P < 0.01）. Using VCG as the standard, ECG missed diagnosis of abnormal indicators of left ventricular electrical activity: 18 T-ring abnormalities, 4 left ventricular high voltage, and 3 branch block. Conclusion VCG has certain diagnostic advantages for early left ventricular abnormality in hypertension, especially for early detection of ventricular repolarization abnormality. Combined application with UCG can improve the positive detection rate of early left ventricular abnormality.
2021, 42(4): 117-121.
doi: 10.12259/j.issn.2095-610X.S20210422
Abstract:
Objective To explore the specific types, occurrence time and age structure of adverse reaction （ADR） in tuberculosis patients after anti-tuberculosis treatment, so as to provide certain reference for rational, safe and effective drug use in clinical practice. Methods The data of 376 patients with adverse reactions due to antituberculosis drugs admitted to Kunming Third People’ s Hospital from January 2015 to December 2019 were collected, and the data were analyzed using SPSS software. Results The occurrence of ADR in patients after anti-tuberculosis treatment was mostly likely to occur in the first 10 days, and the incidence of ADR in males was higher than that in females, but the difference was not statistically significant. The incidence of hepatotoxicity in TB patients after taking rifampicin （RFP） and isoniazid （INH） was higher （P < 0.05）, and the incidence of hyperuricemia in TB patients after taking pyrazinamide （PZA） was higher （P < 0.05）. The incidence of pruritus was higher in patients taking ethambutol （EMB） （P < 0.05）. Conclusion ADRs caused by antituberculosis drugs are mostly hyperuricemia, pruritus, rash, liver damage, etc. Patients may develop ADRs within 10 days of initial dose. Therefore, clinicians should pay special attention to high-risk groups to reduce the occurence of ADR after medication.
2021, 42(4): 134-137.
doi: 10.12259/j.issn.2095-610X.S20210425
Abstract:
Objective To investigate the correlation between urinary iodine and thyroid diseases. Method A total of 266 patients who received physical check-up or treatment in the Department of Endocrinology of our hospital from January to December 2018 were enrolled in the study. Patients were divided into a thyroid disease group （207 cases） and non thyroid disease group （59 cases）; and urine iodine and thyroid stimulating hormone （TSH）, free triiodothyronine （FT3）, and free thyroid Levels of FT4, triiodothyronine （T3）, tetraiodothyronine （T4）, thyroid peroxidase antibody （TPOAb）, thyroglobulin antibody （TGAb） level are detected. Color doppler ultrasound was used to check patients thyroid. SPSS17.0 software was used for t test, rank sum test and χ2 test; and multivariate Logistic regression analysis was used for the incidence factors of thyroid diseases. Results The median urinary iodine level （143.30 ug/L） of the thyroid disease group was significantly lower than that of the non-thyroid disease group （178.30 ug/L, P < 0.05）; multivariate logistic regression analysis results showed that age （OR = 1.140, 95%CI: 1.033-1.257, P = 0.009）, urine iodine （OR = 0.983, 95%CI: 0.971-0.996, P = 0.008） and TPOAb （OR = 1.018, 95%CI: 0.979-1.059, P = 0.020） were independent risk factors for thyroid disease. Conclusion Low levels of urinary iodine are related to the prevalence of thyroid diseases. Urinary iodine, TPOAb and age are independent risks factors of thyroid diseases.
2021, 42(4): 155-160.
doi: 10.12259/j.issn.2095-610X.S20210430
Abstract:
Ultrasound is a non-invasive and safe treatment technique, which has a therapeutic efficacy on most neuropathic pain diseases. Neuropathic pain had been recognized as one of the most difficult pain syndromes in the world. It has a long course and seriously affects the quality of life, cognition, emotion and social function of the patients. With the research development in recent years, ultrasound has great potential in the treatment of neuropathic pain. This article reviews the possible biophysical mechanism of ultrasound therapy and its application in neuropathic pain.
Ultrasound is a non-invasive and safe treatment technique, which has a therapeutic efficacy on most neuropathic pain diseases. Neuropathic pain had been recognized as one of the most difficult pain syndromes in the world. It has a long course and seriously affects the quality of life, cognition, emotion and social function of the patients. With the research development in recent years, ultrasound has great potential in the treatment of neuropathic pain. This article reviews the possible biophysical mechanism of ultrasound therapy and its application in neuropathic pain.
2021, 42(4): 150-154.
doi: 10.12259/j.issn.2095-610X.S20210429
Abstract:
Biofilm extracellular polymeric substances are the basic substances produced by microorganisms in the biofilm formation process, which lead to the enhancement of bacterial pathogenicity, playing an important role in change of microbial behavior, virulence and drug resistance. In this paper, the component, structure, function, research methods of extracellular polymeric substances and treatment strategy of biofilm infection targeting on extracellular polymeric substances are reviewed.
Biofilm extracellular polymeric substances are the basic substances produced by microorganisms in the biofilm formation process, which lead to the enhancement of bacterial pathogenicity, playing an important role in change of microbial behavior, virulence and drug resistance. In this paper, the component, structure, function, research methods of extracellular polymeric substances and treatment strategy of biofilm infection targeting on extracellular polymeric substances are reviewed.
2021, 42(4): 172-175.
doi: 10.12259/j.issn.2095-610X.S20210433
Abstract:
Objective To explore the effect of oral immunotherapy of mother’ s colostrum on very low birth weight premature infants. Methods A total of 100 very low birth weight premature infants born in our hospital from June 2018 to June 2020 were enrolled and randomly divided into two groups. The experimental group （50 cases） received oral immunotherapy with colostrum, while the control group （50 cases） was treated with sterilized water for injection for daily oral care. A prospective controlled study was conducted to evaluate the length of hospital stay, hospitalization cost, enteral feeding time, length of antibiotic therapy, weight gain, incidence of NEC （Necrotizing enterocolitis） and nursing satisfaction of the two groups. Results There were statistically significant differences in the average length of hospital stay, hospitalization cost, enteral feeding time, length of antibiotic therapy, weight gain, incidence of NEC between the two groups （P < 0.05）. The nursing satisfaction degree of the experimental group was significantly higher than control group （ P < 0.05）. Conclusion Oral immunotherapy of maternal colostrum can shorten hospital stay, reduce average daily hospitalization costs, shorten the length of antibiotic therapy, promote the growth and development of infants, reduce the incidence of NEC and improve parental care satisfaction, which is worthy of clinical promotion and application.
2021, 42(4): 168-171.
doi: 10.12259/j.issn.2095-610X.S20210432
Abstract:
Objective Under the concept of ERAS, according to the characteristics of liver cancer disease and perioperative nursing needs, this paper aims to establish the nursing health education during perioperative period of laparoscopic lobotomy and explore its application effect. Methods A total of 160 patients who underwent laparoscopic hepatectomy from January 2020 to December 2020 were randomly divided into control group （n = 80） and experimental group （n = 80）. Control group received traditional nursing and health education during perioperative period, while experimental group received nursing and health education pathways. The time of pre-hospital examination and preoperative preparation, postoperative self-pain assessment, time of activity out of bed, length of hospital stay and patient satisfaction degree were compared between the two groups. Results There was no significant difference in prehospital examination time between the experimental group and the control group （P > 0.05）. Preoperative preparation time, postoperative self-pain assessment, time of getting out of bed and patient satisfaction in the experimental group were significantly better than those in the control group （P < 0.05）. Conclusion Based on the concept of ERAS, the application of nursing health education pathways in the perioperative period of laparoscopic hepatectomy can effectively shorten the length of hospital stay, lower hospitalization costs and reduce the pain.
2021, 42(4): 176-180.
doi: 10.12259/j.issn.2095-610X.S20210434
Abstract:
Objective To explore the feasibility and effectiveness of whole-course nursing management of intestinal polyp operation. Methods A total of 86 cases of intestinal polyp patients who underwent day surgery in our hospital were selected as the experimental group, and 86 cases of intestinal polyp patients who had been admitted to our hospital during the same period for elective surgery were selected as the control group. The experimental group received the whole-course nursing management of day surgery, including the development of multidisciplinary and whole-course nursing management path of day surgery; multiple forms of day surgery education, such as brochures, lectures and WeChat platform. Results The preoperative waiting time, length of hospital stay and direct medical expenses of the experimental group were all lower than those of the control group, with statistically significant differences （P < 0.001）. The patient satisfaction of the experimental group （98.8%） was higher than that of the control group （84.9%）, with statistically significant differences （P = 0.001）. There was no statistically significant difference between the two groups in the rate of intestinal insufficiency and the incidence of postoperative complications （P > 0.05）. Conclusion It is feasible and effective to carry out whole-course nursing management of intestinal polyp operation for day surgery patients, which can increase patient satisfaction and improve patient's medical experience.
2021, 42(4): 146-149.
doi: 10.12259/j.issn.2095-610X.S20210428
Abstract:
Objective To study the effect of acetone in blood samples on the qualitative and quantitative results of ethanol. Methods Headspace gas chromatography （double column and double detector） was used to determine ethanol and acetone in blood samples of 600 suspected drunk drivers or driving after drinking drivers. Results In column 1 （DB-ALC1）, internal standard tert-butanol and acetone could be separated effectively; while in column 2 （DB-ALC2）, acetone could not be separated from the internal standard tert-butanol, which interferes with the accuracy of the ethanol quantitative results. Some blood samples suspected to contain acetone tested in this study were confirmed by GC-MS, and all acetone components were detected positive. The acetone concentration in 113 blood samples were detected by GC. The highest acetone content in the blood samples was 6.55mg/100mL. Conclusion In forensic medical identification, attention should be paid to the influence of acetone on the detection results of ethanol. In order to accurately identify the fact of driving after drinking and drunk driving cases, double-column dual detectors should be used for qualitative determination of ethanol. In the presence of acetone, the mean value of DB-ALC1 column was used as quantitative results.
2021, 42(4): 161-167.
doi: 10.12259/j.issn.2095-610X.S20210431
Abstract:
Objective This paper analyzes the evaluation of the teaching performance of university teachers by the comprehensive index method based on the superior order graph method, and provides reference for the future evaluation of the comprehensive teaching performance of teachers by the teaching and research section. Methods The evaluation system includes three aspects: classroom teaching quality rating, teaching activity participation rate of teaching and research section and course assessment management. The index evaluation system consists of 3 first-level indexes and 9 second-level indexes. The index weight is determined by using the superior order diagram method, and the comprehensive index method is used to comprehensively evaluate the teaching level. Results The weights of classroom teaching quality rating, teaching activity participation rate and course assessment management determined by the optimal sequence diagram were 0.42, 0.22 and 0.36, respectively. The comprehensive index method was used to evaluate the teaching level of 7 teachers from B1-B7 in the teaching and research section. The comprehensive index values were 1.19, 1.11, 1.46, 0.94, 0.99, 0.90, 1.01, and the ranking from high to low was B3 > B1 > B2 > B7 > B5 > B4 > B6. Conclusion The comprehensive index method based on the optimal sequence diagram methods avoided the influence caused by the difference in properties and units of measurement of various indexes, whole added the weight of each index, so as to obtain the comprehensive performance of each teacher in many aspects. This method is simple and feasible, and can be used in the teaching and research section to evaluate the comprehensive teaching performance of university teachers.
