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RSS2022 Vol. 43, No. 10
2022, 43(10): 1-9.
doi: 10.12259/j.issn.2095-610X.S20221021
Abstract:
Objective To screen differential expression gene （DEG） in placental trophoblasts stimulated by vitamin D by second-generation sequencing technology, and to analyze their potential role in the pathogenesis of adverse pregnancy outcomes caused by vitamin D deficiency. Methods The second-generation sequencing analysis was used to detect mRNA expression in placental trophoblasts stimulated by different concentrations of vitamin D （0.1, 1, 10, 100 nm） to screen out DEG, GO and KEGG enrichment analysis was applied, and PPI network gene screening center was constructed. Results Gene expression in four different concentrations of vitamin D stimulated cells of the samples and normal control samples without vitamin D are as follows: a total of 354 DEG were obtained in VitD 0.1 vs Control group, including 242 up-regulated and 112 down-regulated; a total of 320 DEG were obtained in VitD 1 vs Control group, including 216 up-regulated and 104 down-regulated; a total of 374 DEG were obtained in VitD 10 vs Control group, including 277 up-regulated and 97 down-regulated; a total of 300 DEG were obtained in VitD 100 vs Control group, including 151 up-regulated and 149 down-regulated. At last, the intersection of four groups of DEG was taken, and nine common DEGs were obtained. GO analysis showed that biological processes are mainly concentrated in the collagen fibrils tissue, cell differentiation, cell chemotaxis, cell differentiation, the regulation of the negative control, signal receptor activity, outside the cell structure, extracellular matrix organization, protein secretion, adjusting the enrichment of the cellular elements in collagen trimer compounds, proteins of the extracellular matrix, the top part of the cell and collagen trimer; Adjust the molecular function of enrichment in receptor ligands, receptor modulators, protein bridge, the activity of cytokines. KEGG analysis showed significant enrichment in stimulating nerve receptor-ligand interactions, cell adhesion molecules, gastric cancer, cell factor receptor interactions,produce IgA intestinal immune network, inflammatory bowel disease, malaria, amoebiasis and PI3K-Akt signaling pathway. The PPI network identified 17 hub genes, including COL1A2, ACTA2, S100A4, TAGLN, CSF1R, TLR4, TNFSF13B, FTCD, APOBEC3G, IL6, IGF1, PDGFRBTGFB2, BGLAP, COL4A4, COL8A1 and COL11A1. Conclusions COL1A2, ACTA2, S100A4, TAGLN, CSF1R, TLR4, TNFSF13B, FTCD, APOBEC3G, IL6, IGF1, PDGFRBTGFB2, BGLAP, COL4A4, COL8A1 and COL11A1 may be linked to vitamin D deficiency caused by the occurrence and development of adverse pregnancy outcomes, and provide potential therapeutic targets adverse pregnancy outcomes caused by vitamin D deficiency.
2022, 43(10): 10-15.
doi: 10.12259/j.issn.2095-610X.S20221033
Abstract:
Objective To investigate the expression of mTOR in TMJOA condylar cartilage of SD rats. Methods The model of TMJOA in SD rats was established by unilateral mastication, and the corresponding sham-operation group was established. TMJOA model was established by unilateral mastication.The right condyle was taken 2, 4 and 8 weeks after modeling. HE staining and safranine o-solid green staining were used for pathological examination; The expressions of Sox9 and mTOR were detected by immunohistochemical staining. Results Hematoxylin-eosin （HE） staining and saffron o-green staining showed that the condylar cartilage of the control group had clear structure, smooth and intact surface, and the proteoglycans were evenly distributed. In the experimental group （TMD group）, obvious degenerative changes of condylar cartilage were observed over time （P < 0.05）, and the lesions were the highest in 4 W. Immunohistochemical staining showed that the expression level of Sox9 in the condylar cartilage of the experimental group decreased successively at 2 W （ P < 0.01）, 4 W （ P < 0.05） and 8 W （ P < 0.05）, and were lower than that of the control group. The expression level of mTOR decreased at 2 W （ P < 0.05） and 4 W （ P < 0.001）, but increased relatively at 8 W （ P < 0.05） . Conclusion With the progress of inflammation, mTOR in TMJOA condylar cartilage of SD rats shows a trend of early down-regulation and late up-regulation, which plays a key role in transforming protective autophagy into destructive apoptosis.
2022, 43(10): 16-21.
doi: 10.12259/j.issn.2095-610X.S20221015
Abstract:
Objective To analyze the trends in prevalence, awareness, treatment, and control of diabetes mellitus （DM） among NaXi ethnic minority people in Yunnan Province. Methods A repeated cross-sectional design was adopted, and a total of 1402 permanent NaXi residents aged ≥35 years in 2013 and 1426 in 2018 were selected by multi-stage stratified random sampling. Participants received a questionnaire survey and physical examination. Results In 2018, the prevalence of prediabetes and DM among the surveyed population were 19.5% and 8.6%, while the awareness, treatment, and control of diabetes were 52.8%, 89.2%, and 29.2%, respectively. From 2013 to 2018, there was no significant change in the prevalence of pre-diabetes among the surveyed population, whereas the prevalence of prediabetes among the elderly aged 65 years and over, those with junior high school educational level and above, and those with high annual household income increased from 21.5%, 17.7%, and 17.9% to 28.9%, 22.3%, and 23.6%, respectively （all P < 0.05）. The prevalence of DM increased from 4.1% to 8.6% （P < 0.001）. And the prevalence of DM among the subgroups categorized by sex, age, education level, and income level this increasing rate was also observed in upward trends （all P < 0.05）. The awareness rate of DM decreased from 69.0% to 52.8% （P < 0.05）. There was no significant change in treatment and control rate of DM （all P > 0.05）, whereas the rate of treatment among diabetic persons who are female, aged 55-64 years and persons with primary school educational level and below, and persons with low annual household income increased from 71.4%, 62.5%, 57.9%, and 71.4% to 93.9%, 95.0%, 88.9%, and 90.5% respectively （all P < 0.05）. Conclusion The prevalence of DM among NaXi residents shows an increasing trend, while the awareness rate shows a decreasing trend. There is no significant change in the treatment rate and control rate. It should take specific effective measures to reduce the prevalence of prediabetes and DM, and improve the awareness rate and control rate, so as to delay the occurrence and development of DM.
2022, 43(10): 22-27.
doi: 10.12259/j.issn.2095-610X.S20221018
Abstract:
Objective To explore the effects of which Panax noto-ginseng saponins （PNS） influence the proliferation of human stem cells from apical papilla （hSCAPs）. Methods We collected apical papilla and separated primary cells with wall methods. Flow cytometry was used to identify the surface markers of hSCAPs, the multi-directional-differentiation potential of hSCAPs was identified by inducing bone and fat differentiation. PNS-culture-medium with 25 mg/L, 50 mg/L, 100 mg/L, and 200 mg /L was prepared, CCK-8 method was used to detect the proliferation rate of hSCAPs under different concentrations of PNS, and the changes of the cell cycle at the best proliferation concentration were observed. Results hSCAPs was cultivated successfully by primary separation and belong to the source of mesenchymal stem cells. The related surface markers were highly expressed and induced to fat differentiation and bone differentiation. Compared with the control and other experimental groups, PNS with 25 mg/L promoted highly hSCAPs proliferation. The difference was statistically significant （P < 0.05）. Conclusions PNS with 25 mg/L has the best effect for boosting hSCAPs proliferation abilities. It is prompted that hSCAPs has a strong sensitivity to PNS, that is, a low concentration can significantly promote the proliferation of hSCAPs.
2022, 43(10): 28-34.
doi: 10.12259/j.issn.2095-610X.S20221002
Abstract:
Objective To observe whether breviscapine has any effect on the mRNA and protein levels of CINC-1 and CINC-2 in the right ventricle of rats with pulmonary embolism. Methods The rat model of pulmonary embolism was replicated, and rats in the breviscapine low-dose, high-dose and low-molecular-weight heparin groups were given intraperitoneal injection of corresponding drugs, lung tissue samples were collected for pathological detection of pulmonary embolism, and right ventricular tissue samples were determined by Q-PCR and Western blot to determine CINC-1, CINC-2 gene and protein levels in each group. Results Compared with the sham operation group, the gene expression of CINC-1 and CINC-2 in the model group was significantly upregulated （P < 0.01）. The low-dose, high-dose and low-molecular-weight heparin groups of breviscapine could significantly down-regulate the gene expression of CINC-1 and CINC-2 （P < 0.01）. The high-dose breviscapine group was better than the low-molecular-weight heparin group in down-regulating CINC-1 gene level （P < 0.05）. Compared with the sham operation group, the protein expressions of CINC-1 and CINC-2 in the model group were significantly increased （P < 0.01）. The low-dose and high-dose groups of breviscapine could significantly down-regulate the protein level of CINC-1 （P < 0.01）, and the high-dose group of breviscapine was better than the low-molecular-weight heparin group in down-regulating the protein expression of CINC-1 （P < 0.05）. And the high dose of breviscapine can significantly down-regulate the protein level of CINC-2 （P < 0.01）. Conclusion Breviscapine may play an anti-inflammatory role in pulmonary embolism by down-regulating the gene and protein expression of CINC-1 and CINC-2 in the right ventricle of rats with acute pulmonary embolism.
2022, 43(10): 35-42.
doi: 10.12259/j.issn.2095-610X.S20221022
Abstract:
Objective To investigate the mechanism of HOXA9 in the process of progesterone inhibition of ovarian cancer progression. Methods （1） Human ovarian cancer cell line HO-8910 were cultured according to the gradient of progestin medroxyprogesterone acetate （MPA） concentration （0.1, 1, 10, 50 and 100 μmol/L）, and the cell morphology was observed after 24 h, 48 h and 72 h. CCK-8 was used to detect cell viability and determine IC50. The apoptosis rate was detected by Annexin V-FITC double staining. （2） H0-8910 were divided into four groups: 1） NC group; 2） progestin group; 3） progestin+si-HOXA9 group; 4） progestin+ov-HOXA9 group. H0-8910 cells were transfected with HOXA9 knockdown or overexpression plasmids and treated with MPA or not transfected with plasmids alone using MPA for 72 h. The qPCR and western blot were used to detect the mRNA and protein expression of HOXA9. as well as the expression of epithelial mesenchymal transition （EMT）-related proteins （Vimentin, N-cadherin, E-cadherin）, respectively. Wound scratch assay was used to detect cell migration; Transwell assay was used to detect cell invasion. Results （1） After progesterone stimulation, the growth status of HO-8910 became poor, the arrangement was sparse, cell proliferation, migration and invasion ability were suppressed （P < 0.0001）, and the apoptosis rate increased （P < 0.0001）; （2） calculated by cck8 results, the IC50 value of H0-8910 cells treated with MPA for 72 h was 2.680 μmol/L; （3） compared with human ovarian epithelial cells CP- H055, HOXA9 mRNA was highly expressed in human ovarian cancer cell lines HO-8910, SKOV3, A2780 and OVCAR3 （P < 0.01）, and HOXA9 protein was likewise highly expressed in HO-8910 （P < 0.001）; （4） progesterone could inhibit HOXA9 expression （P < 0.0001）, and the inhibition of cell invasion and migration ability by progesterone was more obvious after knocking down HOXA9 （P < 0.0001）, while the inhibition of HO-8910 invasion and migration ability was slightly reduced after overexpression of HOXA9 （P < 0.0001）. （5） Progesterone increased E-cadherin protein expression （P < 0.01）, while Vimentin and N-cadherin protein expression decreased （P < 0.001）; after knocking down HOXA9, the effect of progesterone on EMT-related protein expression in HO-891 was more obvious （P < 0.05）, while after overexpressing HOXA9, the effect of progesterone on EMT-related protein expression in HO-891 was weakened （P < 0.05）. Conclusion Progesterone inhibits the development of ovarian cancer by suppressing the expression of HOXA9 gene and therefore suppressing the malignant biological phenotypes such as cell proliferation, migration, invasion and EMT process.
2022, 43(10): 43-48.
doi: 10.12259/j.issn.2095-610X.S20221008
Abstract:
Objective To understand the epidemic situation of brucellosis in these areas, so as to provide a reference for clinical early prevention and diagnosis through the epidemiological analysis of brucellosis in some areas of Yunnan. Methods The epidemiology and clinical manifestations of brucellosis patients treated in Kunming Third People's Hospital from November 2020 to February 2022 were analyzed retrospectively. Results The 175 cases were mainly in Kunming （41.71%） and Qujing （38.86%）; The age was concentrated in 41～60 years old （53.14%）, and the ratio of men and women was 2.13∶1. Cattle and sheep breeders were more common （65.71%）; Most of them entered the living environment of cattle and sheep for a long time （99.43%）; The peak incidence was from October to December （36.57%）. Fever accounted for 67.43%, night sweat 82.29%, joint pain 85.71% （low back pain 60.00%）. 1.14% had no clinical symptoms. Spinal lesions accounted for 90.48% of complications. Test tube agglutination test （SAT） was positive in all patients, and the titer was between 1∶50++～1∶3200. The positive rate of Brucella Maltese cultured in blood was 39.43% （69 / 175 cases）; The positive rate of bone marrow culture was 38.55% （32 / 83 cases）. Conclusion This group of cases mainly comes from Kunming and Qujing areas, which often occur from October to December. They are mainly infected in the living environment of cattle and sheep. The bone lesions of the 4th and 5th lumbar vertebrae are the most common complications.
2022, 43(10): 49-56.
doi: 10.12259/j.issn.2095-610X.S20221031
Abstract:
Objective To study the therapeutic effect of multimedia visual function training on amblyopia in children and explore the factors influencing the therapeutic effect. Methods Sixty-three patients with refractive amblyopia aged 3-12 years （87 eyes） were randomly divided into the observation group and the control group. The observation group received multimedia comprehensive visual function training, and the control group received traditional fine visual training for 30 min/d. A total of 6 months of follow-up were performed. Best corrected visual acuity （BCVA）, Titmus near-stereoscopic vision, pVEP and Worth four-point lamp were examined before treatment, 1 month, 3 months and 6 months after treatment, respectively. The differences of monocular visual acuity and binocular visual function in children with amblyopia were compared between the two training methods. Correlation analysis was used to explore the factors influencing the therapeutic effect of multimedia visual function training. Results 1. BCVA in the observation group and the control group increased 6 months after treatment compared with that before treatment, and the increase value in the observation group was greater （P < 0.001）. For mild and moderate amblyopia, amblyopia, ametropia and anisometropia in children aged 3-6 years and 7-12 years, BCVA in observation group was higher than that in control group （P < 0.05）. 2. The improvement of Titmus near stereo vision and interocular inhibition in the observation group was better than that in the control group （P < 0.05）. 3. The amplitude of pVEP P100 wave （15’frequency） in the observation group increased after treatment compared with before treatment （P < 0.001）. 4. Factors influencing the therapeutic effect of multimedia visual function training: BCVA increased value was positively correlated with LogMAR visual acuity before treatment, negatively correlated with age; The cure rate of Titmus near stereo was positively correlated with Titmus near stereo before treatment, but negatively correlated with binocular anisometropia. Conclusions 1. Multimedia comprehensive visual function training is better than traditional fine visual training in improving monocular vision and binocular visual function in amblyopia children. 2. Factors influencing the therapeutic effect of multimedia visual function training: BCVA improvement was correlated with the visual acuity before treatment and age; The cure rate of Titmus near stereo vision was correlated with the Titmus near stereo vision and binocular anisometropia before treatment.
2022, 43(10): 57-61.
doi: 10.12259/j.issn.2095-610X.S20221019
Abstract:
Objective To review the data of carpal tunnel release surgery in the treatment of carpal tunnel syndrome （CTS） with carpal arthroscopy, and to explore the clinical effect of this operation. Methods 47 patients with carpal tunnel syndrome admitted in our department between December 2020 and December 2021 as the object in this study. All patients were treated with carpal tunnel syndrome by single-channel carpal tunnel release surgery under carpal arthroscopy, and all patients were followed up at least for 6 months. The recovery of symptoms and function before and after surgery was compared using the Modified Boston Carpal Tunnel Syndrome Score Scale （SSS score and FSS score）. Results After follow-up investigation, the symptoms of hand anesthesia and function in 47 patients were significantly improved compared with those before surgery, and the incidence of postoperative wrist pain was small. SSS score was （47.53±2.2） before and （82.13±9.42） after surgery, and the difference was statistically significant （P < 0.05）. FSS score was （10.00±1.12） before and （30.76±2.64） after surgery, and the difference was statistically significant （P < 0.05） . Conclusion Carpal arthroscopy single channel carpal tunnel release in the treatment of carpal tunnel syndrome can achieve reliable clinical efficacy, can significantly relieve patients with hand anesthesia and other symptoms, can reduce complication of postoperative pain and improve hand function, is worth popularizing in clinical use.
2022, 43(10): 62-65.
doi: 10.12259/j.issn.2095-610X.S20221029
Abstract:
Objective To observe the changes of ANP levels in the peripheral blood of patients with essential hypertension in Yunnan, and to explore the relationship between it and the pathogenesis of essential hypertension. Methods We selected 44 patients with essential hypertension diagnosed in the cardiology department of our hospital from June to September 2019 and set them as the case group. We also selected 44 healthy patients from the physical examination center of our hospital as the healthy control group. The ELISA method was used to detect the ANP level in the peripheral blood of the subjects. Results The level of ANP in the peripheral blood of patients with essential hypertension was significantly lower than that of normal people（P < 0.05）; the occurrence of hypertension was used as the dependent variable and the serum ANP was used as the independent variable for logistic regression analysis. The results showed that ANP The partial regression coefficient with hypertension was 0.071, and the P value was 0.002（ P < 0.05）. It was statistically significant; the person correlation analysis was used to analyze ANP and SBP/DBP, and the results showed that the correlation coefficient between ANP and SBP was -0.374. The P value is 0.000（ P < 0.05）; the correlation coefficient between ANP and DBP is -0.331, and the P value is 0.002（ P < 0.05）, both of which are statistically significant, but compared to DBP, the correlation between ANP and SBP is more significant. Conclusions The low concentration of ANP in peripheral blood is closely related to the increase of blood pressure; NPR-C ligand ANP is involved in the pathogenesis of essential hypertension; ANP affects the changes in the level of SBP/DBP, and has a more significant impact on SBP.
2022, 43(10): 66-73.
doi: 10.12259/j.issn.2095-610X.S20221016
Abstract:
Objective Cross-sectional study of the characteristics of cardiac color ultrasonography and carotid ultrasonography in different age groups of patients with HIV/AIDS. Methods This study collected data from HIV/AIDS patients, who attended the Department of Infection I at the Third People’s Hospital of Kunming from December 1, 2020 to November 30, 2021, and the patients underwent the cardiac color ultrasonography and carotid ultrasonography. Patients was divided into 98 cases （28.0%） in the young group （≤44 years old） and 166 cases （47.4%） in the middle-aged group （45-59 years old） and 86 cases （24.6%） in the elderly group （≥60 years old）, according to the age grouping criteria published by WHO, and the data were organized and analyzed using Excel and SPSS26.0. Results In accordance with our study, there were statistically significant differences between age groups in terms of ethnicity, smoking, route of transmission, presence of hypertension, diabetes mellitus, and presence of hepatitis and （P < 0.05）; the abnormalities of cardiac color ultrasonography and carotid ultrasonography were different in different age groups （P < 0.05）. Additionally, there was a gradually increasing trend in the abnormalities of ultrasound examination （χ2 trend were 107.148, 77.666, all P trend values < 0.05）. Among the abnormal findings of cardiac color ultrasonography and carotid ultrasonography in different age groups , there were statistically significant differences in the incidence rate of the following indicators （P < 0.05）, such as left ventricular diastolic dysfunction, aortic calcification, elevated pulmonary artery systolic pressure, aortic regurgitation, ventricular septal thickening, increased right and left atrial internal diameter, increased right ventricular internal diameter, mitral valve calcification, presence of plaque in the neck, whether there was thickening of the intima-media thickness of the carotid arteries bilaterally, the peak flow velocity of the right internal carotid artery and bilateral internal carotid artery resistance index. Of those, the incidence rate of left ventricular diastolic dysfunction was most common. Conclusions For patients with HIV/AIDS in different age groups, differences in the abnormal findings and abnormal presentation of cardiac color ultrasonography and carotid ultrasonography. Based on the clinical manifestations and ultrasound findings of the patients, clinicians should make timely diagnosis of cardiovascular diseases associated with HIV/AIDS patients in different age groups.
2022, 43(10): 74-78.
doi: 10.12259/j.issn.2095-610X.S20221028
Abstract:
Objective To predict the probability of occult lymph node metastasis （OLM） in the central region of the neck by analyzing the dual-energy CT energy spectrum curve of thyroid papillary carcinoma （PTC）. Methods Patients with PTC confirmed by pathology before and after operation were retrospectively collected. CT images were analyzed by radiologists. There were 140 cases without cervical lymph node metastasis. Among them, 88 patients with occult lymph node metastasis in the central group （OLM+） and 52 patients without metastasis （OLM−） were confirmed by pathology. The CT value of PTC on each single energy image was measured and the slope （k） of the energy spectrum curve was calculated. The differences in parameters between the two groups were analyzed. Results （1） the karterial, 40 keVarterial, 40 keVvenous, 160 keVvenous and 180 keVvenous of PTC between OLM+ group and OLM− group in arterial and venous phase were significantly different （P < 0.05）. （2） The AUC of 40 keVarterial, karterial, 40 keVvenous, 160 keVvenous and 180 keVvenous in PTC were 0.590, 0.622, 0.590, 0.429 and 0.424 respectively. When karterial = 2.563, the sensitivity and accuracy of diagnosing PTC with OLM in the central group were 93.2% and 69.3%. When 40 keVvenous = 370.25, the specificity of PTC with OLM was 86.5%. Conclusion The energy spectrum curve of PTC has a certain reference value in predicting occult lymph node metastasis in the central region of the neck. karterial has the highest sensitivity and 40 keVvenous has the highest specificity.
Analysis of the Status and influencing Factors of Iron Deficiency Anemia During Pregnancy in Kunming
2022, 43(10): 79-83.
doi: 10.12259/j.issn.2095-610X.S20221011
Abstract:
Objective To analyze the prevalence and influencing factors of iron-deficiency anemia among women in different pregnancy stages in Kunming, as so to provide a scientific basis for the prevention and treatment of the disease. Methods A questionnaire survey combined with clinical data and laboratory test results were used to statistically analyze the prevalence of ID, IDA, cognitive behavior, and influencing factors among 493 pregnant women who gave birth in Yunnan First People’s Hospital from October 2021 to March 2021. Results The prevalence rates of ID and IDA in the second trimester were the highest, and pregnant women had incomplete knowledge about scientific iron supplementation. Factors such as educational background, income, parity, pre-pregnancy BMI, and knowledge and behavior of scientific iron supplementation were related to iron deficiency anemia during pregnancy （P＜0.05）. Conclusions The prevalence rate of ID and IDA during pregnancy in Kunming Surveillance Hospital is at a low level, but it is still necessary to combine modern online teaching tools to strengthen the education of iron supplementation-related knowledgein maternity and obstetrics. Referring to international experience, preventive iron supplementation is expected to bring new breakthroughs in the prevention of this disease.
2022, 43(10): 84-89.
doi: 10.12259/j.issn.2095-610X.S20221017
Abstract:
Objective To explore the imaging features of 64-row, 128-slice spiral CT of the normal adult coracoid process to provide a reference for clinical coracoid process safety surgery. Methods The data of 121 adult patients who underwent 64-row 128 slice spiral CT scanning of shoulder joints from January 2019 to May 2022 in Yunnan Provincial Hospital of traditional Chinese medicine were randomly selected as samples. The sagittal plane of the shoulder joint was taken as the measurement object to measure the length of the coracoid process and coracoid process knee, the distance from the coracoid process tip to the midpoint of coracoid process knee, and the height of the coracoid process base and coracoid process tip, and then the sagittal plane of the shoulder joint was taken as the measurement object to measure the base of coracoid process tip Width of coracoid process knee and coracoid process base; The measurement results were statistically analyzed by spss27.0 statistical software package. The basic description of normal distribution measurement data was expressed by mean, median, standard deviation, maximum value, minimum value and \begin{document}$\bar x \pm s $\end{document} ![]()
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Results The length of the coracoid process is 59.92±6.11 mm; the length of the coracoid knee is 14.05±2.26 mm; the distance from the coracoid tip to the midpoint of the coracoid knee is 22.59±3.65 mm; the height of the base of the coracoid tip is 9.86±1.71 mm; the height of the coracoid tip is 7.22±1.79 mm; the width of the coracoid tip is 9.14±2.08 mm; the width of the coracoid knee is 13.79±2.70 mm; the width of the coracoid base is 21.10±4.18 mm; Sample t-test, in addition to the height of the coracoid process tip, the coracoid image measurement distance between male and female patient groups was statistically significant （P < 0.05）; the coracoid process length, coracoid process tip between left and right patient groups The distance to the midpoint of the coracoid knee and the width of the coracoid knee were statistically significant （P < 0.05）; the length of the coracoid process in males and females and the length of the coracoid process to the midpoint of the coracoid knee were statistically significant （P < 0.05）. The distance, the width of the coracoid tip and the width of the coracoid knee were all correlated （P < 0.05）. Conclusion The length and width of the coracoid process are different from male and, gender and left and right, among which, the length of the coracoid process and the distance from the tip of the coracoid process to the midpoint of the knee of the coracoid process, the width of the tip of the coracoid process and the width of the knee of the coracoid process are different in both male and female. There are certain correlations; therefore, attention should be paid to gender, left and right, and the correlation between them during clinical coracoid surgery or the design of related equipment and consumables.
2022, 43(10): 90-97.
doi: 10.12259/j.issn.2095-610X.S20221025
Abstract:
Objective To analyze the clinical characteristics of 498 adult patients with particularly severe burns. Methods We made a retrospective analysis of the medical records of the second affiliated hospital of Kunming medical university in January 2016 December 2020 498 cases with inclusion criteria, the gender, age, occupation, burn cause, burn area, burn depth, depth of hospital treatment, emergency treatment, compound injury, complications, surgical treatment, treatment, hospital time, hospital costs, medical payment. The data were performed χ2 test, Mann-Whitney U test, Kruskal-Wallis H test, Fisher exact probability method analysis, and Binary Logistic regression analys is）. Results A total of 6, 023 hospitalized burn patients were admitted during the past five years, of which 498 patients （8.27%） were adults with particularly severe burns who met the inclusion criteria. （1） injuries were mainly caused by 462 （92.77%）, with the most in 367; significantly more men than women （χ2 = 155.189, P < 0.001）; concentrated in youth group, 289（58.03%）, more in rural areas than cities （χ2 = 33.936, P < 0.001）; occupation mainly farmers （251 cases, 50.40%） and workers （121, 24.30%）. （2） After injury, 62 patients （12.45%） and 436 patients （87.55%） were not washed with cold water. The cure rate of patients with cold water washing was higher than that of unwashed patients, and the mortality rate was lower than that of unwashed patients. （3） With 340 complications, the top five were inhalation injury （56.18%）, hypovolemic shock （26.47%）, hypoproteinemia（18.24%）, pulmonary infection （17.35%）, and sepsis （12.06%）. The main causes of death were mostly inhalation injury and sepsis and multiple organ failure. （4） treated 320 （64.26%）, 178 （35.74%）; 348 （69.88%）, 49 （9.84%）, 101 （20.28%）, and different treatment methods （Ζ = −8.310, P < 0.001）. （5） The overall average of hospital stay was 36 （15, 62） d. The total hospitalization cost per capita is: 140, 740.2250 yuan.The highest payment method was in 335 cases （67.27%）. （6） In age, sex, total burn area, III burn area, presence of complications, and length of hospitalization affected the outcome of patients with severe burns, which were significant （P < 0.05）. Conclusions The patients with severe burns were more male than female, and heavy manual workers accounted for the main part. According to the epidemiological characteristics of this area, the effective popularization and education of relevant burn emergency knowledge can create favorable conditions for subsequent treatment. Following the development of medicine, using new technologies to effectively treat complications related to particularly severe burns, it is recommended to use multidisciplinary treatment as the leading collaborative treatment to improve the overall treatment level. Strengthen the publicity of medical insurance policies, advocate the active participation of all people in the insurance, reduce the proportion of self-funded patients, and reduce the burden on their families.
2022, 43(10): 98-103.
doi: 10.12259/j.issn.2095-610X.S20221027
Abstract:
Objective To compare the clinical significance of brucellosis spondylitis imaging in Yunnan province. Methods The data of 38 patients with Brucella spondylitis confirmed by clinical and laboratory examination in our hospital from January 2014 to November 2021 were collected, all of whom were local residents of Yunnan province. The data obtained from MRI and CT examination of the patients for the first time （37 cases of MRI examination, 26 cases of CT examination, 25 cases of MRI and CT examination at the same time） were retrospectively analyzed. Results In this study, 37 cases （97.4%） were involved in lumbar vertebrae, 37 cases （97.4%） were involved in multiple vertebrae, and 1 case （2.7%） only showed abscess formation at the posterior margin of vertebrae. It was found that the lesions mainly occurred at the upper and lower edges of the vertebral body, and the L4-5 vertebral bodies were most frequently involved, accounting for 44.7% （17/38）. One case （2.7%） had flattening of two affected vertebrae and four cases （10.5%） had mild spondylolisthesis of the affected vertebrae. 35 cases （92.1%） were associated with different degrees of bone destruction. Thirty-six patients （94.7%） suffered intervertebral disc damage. 24 cases （63.2%） had paraspinal and/or epidural abscess, and 2 cases （8.33%） had paraspinal abscess involving the right psoas major muscle. Conclusions The CT and MRI imaging manifestations of sporadic Brucella spondylitis in Yunnan are diverse, and the involvement of dead bone and abscess into the psoas major muscle and epidural abscess formation alone cannot completely exclude the disease. It is of guiding significance and reference value to clinical diagnosis and treatment.
2022, 43(10): 104-109.
doi: 10.12259/j.issn.2095-610X.S20221013
Abstract:
Objective To explore the advantages and application value of combined intervention of thoracic surgery and rehabilitation department in fast track surgery after uniportal video-assisted thoracoscopic surgery. Methods Patients who underwent uniportal video-assisted thoracoscopic surgery（including lung wedge resection, segmentectomy, and lobectomy） in the Anning First People’s Hospital was divided into two groups. From February 2020 to February 2021, 105 patients were in the control group（postoperative routine thoracic surgery treatment）, 125 patients from March 2021 to December 2021 were in the research group（postoperative thoracic surgery combined with rehabilitation department treatment group）, and the surgical data, hospitalization data, and nursing-related data of the patients were collected. The differences in postoperative hospital stay, postoperative pain visual analog scales（VAS） scores, and patient satisfaction were compared between the two groups. Results The postoperative hospital stay time of the study group was lower than that of the control group, and the difference was statistically significant（P < 0.05）, and the VAS score at 24h and 48h after the operation were lower than that of the control group, and the difference was statistically significant（ P < 0.05）, 72h VAS score after the operation, lower than the control group, the difference was not statistically significant（ P > 0.05）. Satisfaction was higher than that of the control group, and the difference was statistically significant（ P < 0.05）. Conclusions The combined intervention of thoracic surgery and rehabilitation department can further relieve pain, reduce the dosage of analgesic drugs, and shorten the postoperative hospital stay after uniportal video-assisted thoracoscopic surgery. Under the concept of fast-track surgery, the combined intervention of thoracic surgery and rehabilitation department has significant advantages in uniportal video-assisted thoracoscopic surgery, which is worthy of clinical application.
2022, 43(10): 110-114.
doi: 10.12259/j.issn.2095-610X.S20221014
Abstract:
Objective To investigate the preoperative diagnostic value of 3.0T magnetic resonance cholangiopancreatography （MRCP） in the diagnosis of Pancreaticobiliary maljunction （PBM） in children. Methods A retrospective analysis of 25 cases of children with PBM （8 males and 17 females; age 1 month 3 days to 14 years old） of 25 children with PBM confirmed by the Guangdong Worman and Children Hospital were conducted. We reconstructed the image data and compared it with the intraoperative findings and intraoperative cholangiography. Two radiologists at or above the deputy chief physician analyzed the images, and then discussed the results of the imaging diagnosis. Results Among the 25 children diagnosed with PBM, conventional MR and MRCP accurately showed 18 of them （the coincidence rate was 72%）, and intraoperative cholangiography accurately showed 22 cases （the coincidence rate was 88%）. There was no statistically significant difference in coincidence rate （P > 0.05）. Among them, 16 cases of MRCP showed consistent with intraoperative cholangiography, which can clearly show PBM, pancreatic duct, intrahepatic bile duct, and common bile duct, which can clarify the abnormal classification of PBM. Among the 25 children with PBM, 16 cases were type Ⅰ, 5 cases were type Ⅱ, 3 cases were type Ⅲ, and 1 case was type Ⅳ. Conclusion 3.0T MR is an effective method for non-invasive diagnosis of PBM in children. It complements intraoperative cholangiography to achieve the purpose of preoperative diagnosis rate.
2022, 43(10): 115-121.
doi: 10.12259/j.issn.2095-610X.S20221001
Abstract:
Objectives To explore the relationship between the increase of Gamma-glutamyl transferase （GGT） and dyslipidemia in checkup population. Methods 3137 subjects were selected from April 2020 to August 2021. The baseline data, GGT, and four serum lipid indexes of total cholesterol （TC）, triglyceride （TG）, high lipoprotein （HDL） and low density lipoprotein （LDL） were collected. First of all, according to the quartile of GGT concentration, the levels of the four indexes of blood lipids were compared; secondly, the correlation between the concentration of GGT and the four indexes of blood lipids was analyzed; again, the differences of GGT levels among different groups of dyslipidemia were compared; finally, the risk factors affecting the increase of GGT were screened out by binary Logistic regression analysis. Results With the increase of GGT concentration, the abnormal rate and abnormal level of TC, TG and LDL increased significantly, and the abnormal rate of HDL increased and the abnormal level decreased significantly, and the differences were statistically significant （all P < 0.05）; Spearman correlation analysis showed that GGT was positively correlated with TC, TG and LDL （r = 0.200, 0.395, 0.186, all P < 0.05）, and negatively correlated with HDL （r = -0.144, P < 0.05）; The concentration of GGT increased with the increase of abnormal indexes in the four indexes （all P < 0.05）; The multivariate analysis of binary Logistic regression showed that TG was an independent risk factor for the increase of GGT concentration, regardless of the median or reference value of GGT positive, and the OR values were 2.360 （95%CI: 2.068～2.695） and 1.635 （95%CI: 1.481～1.805）, respectively. Conclusions The increase of GGT can be caused by the disorder of blood lipid metabolism, and the degree of increase is closely related to the severity of dyslipidemia. Persistent elevated GGT associated with dyslipidemia may be helpful in the diagnosis and treatment of dyslipidemia-related diseases.
2022, 43(10): 122-126.
doi: 10.12259/j.issn.2095-610X.S20221032
Abstract:
Objective To analyze the efficacy of emergency endoscopic hemostasis in patients with anastomotic hemorrhage after surgical resection of colorectal cancer. Methods The clinical data of patients with anastomotic hemorrhage after surgical resection of colorectal tumor and emergency endoscopic hemostasis were collected from January 2005 to December 2021 in 908th hospital of The People's Liberation Army Joint Logistic Support Force. The demographic data, tumor site, tumor stage and pathological type were analyzed. Of which, the time of anastomotic hemorrhage, hemostasis methods, effects and complications of endoscopic treatment, and the reasons for the failure of endoscopic hemostasis were analyzed selectively. Results Among 1 058 patients undergoing colorectal cancer surgery, 36 patients had anastomotic bleeding （3.4%）. The left colon and rectum cancer were the most patients with hemorrhage （83.3%）. In terms of tumor staging and differentiation, most of the patients were from stage Ⅰ to stage Ⅲ （82.1%）. The pathology was dominated by highly and moderately differentiated cancers （86.1%）. Anastomotic hemorrhage occurred within 72 hours after surgical resection in 26 patients （72.2%）, which was the time of high incidence of hemorrhage. Among the 36 patients, endoscopic hemostasis was successful in 32 cases （88.9%）, in which titanium clip and electrocoagulation combined with titanium clip were the main hemostasis methods （88.9%）. Beside, 4 patients failed to hemostasis by endoscopy, all of them had anastomotic leakage and could not identify the exact bleeding site, and all successfully hemostasis by surgical treatment. No serious complications such as anastomotic leakage, abdominal abscess or death occurred in all 36 patients. Conclusion The peak period of anastomotic hemorrhage is within 72 hours after surgical resection for colorectal cancer. Emergency endoscopic hemostasis is a safe and effective treatment for anastomotic hemorrhage.
2022, 43(10): 127-130.
doi: 10.12259/j.issn.2095-610X.S20221004
Abstract:
Objective To study the effect of radiofrequency therapy on anterior vaginal wall prolapse with SUI. Methods Clinical data of 37 patients with I-II degree of anterior vaginal wall prolapse with SUI in Kunming Maternal and Child Health Hospital were analyzed. The patients were treated with radiofrequency therapy （50 minutes each time, 5 times for one course of treatment）, and were followed in January and March later. The simple impact questionnaire on pelvic floor disorders 7 （PFIQ-7）, pelvic organ prolapse/incontinence function questionnaire （PISQ-12）, and pelvic floor muscle strength grade were evaluated. Results POP-Q scores of Aa and Ba were better than before treatment, the difference was significant （P < 0.05）, PFDI-12, PFIQ-7 scores and before treatment, （P < 0.05）; 76.4% had pelvic floor muscle strength above grade 3. Conclusion RF has significant clinical efficacy in the treatment of anterior vaginal wall prolapse I-with SUI.
2022, 43(10): 131-135.
doi: 10.12259/j.issn.2095-610X.S20221007
Abstract:
Objective To investigate the effect of the environmental sanitation improvement on the positive rate of environmental microbe culture and the body fluid culture of hospitalized drug abusers in an addiction treatment center. Methods From February 2018 to December 2021, a total of 644 microbial culture samples were collected from 221 hospitalized drug abusers in an addiction treatment center. Meanwhile, environmental microbe samples were collected from the prison area in the same center during the same period to analyze whether the positive culture rate of the two sets of samples changed after the improvement of environmental sanitation in this center. Results The positive rate of environmental microbe culture after environmental sanitation improvement was significantly lower than that before environmental sanitation improvement; After environmental sanitation was improved, the positive rate of Klebsiella pneumoniae and acid-fast bacillus culture was significantly lower than before （P < 0.05）; After environmental sanitation was improved, the positive culture rates of Staphylococcus aureus, Escherichia coli, and Staphylococcus epidermidis were markedly decreased （P < 0.05） than before. Conclusions Improvement of environmental sanitation in the prison area of an addiction treatment center can effectively reduce the positive culture rates of both environmental microbe and inpatients' body fluid samples.
2022, 43(10): 136-138.
doi: 10.12259/j.issn.2095-610X.S20221026
Abstract:
Objective To study the expression of CRP, IL-6 and TNF-a in the serum of patients with Adolescence polycystic ovary syndrome （PCOS）. Methods Patients with Adolescence polycystic ovary syndrome（PCOS）were selected as the observation group （100cases）, and 70 routine medical examination Adolescence women as the control group. The levels of CRP, IL-6, TNF-a, fasting plasma glucose （FPG）and fasting serum insulin （FINS） were detected Insulin Resistance Index （HOMA-IR） was calculated. Results Serum levels of CRP, TNF-a, IL-6 and HOMA-IR in PCOS group were significantly higher than those in the control group （P < 0.05）. The levels of CRP, TNF-a and IL-6 in the obese PCOS group were higher than those in the non-obese PCOS group （P < 0.05）. Conclusion The serum levels of CRP, TNF-a and IL-6 were significantly increased in Adolescent PCOS patients, which suggested that inflammatory factors might be involved in the pathogenesis of Adolescence polycystic ovary syndrome.
2022, 43(10): 139-142.
doi: 10.12259/j.issn.2095-610X.S20221012
Abstract:
Objective To explore the occurrence of neonatal respiratory distress syndrome in different Delivery Modes by analyzing the graded differences in the chest X-ray imaging performance of neonatal respiratory distress syndrome in different Delivery Modes and to improve the clinical understanding of the disease. Methods According to the diagnostic and grading criteria of chest X-ray, the chest X-ray manifestations of 133 cases of neonatal respiratory distress syndrome confirmed by clinical consultation in our hospital from 2015 to 2021 were retrospectively analyzed, and the gestational age, weight and mode of delivery of the children were recorded. Results In the children with NRDS of different delivery modes, the number of chest X-ray image gradesⅡand Ⅳ in the cesarean section group was significantly higher than that in the vaginal delivery group. The chest X-ray image grades of the children with two different delivery modes were different, and there was statistical significance （P < 0.05）. The severity of NRDS was more severe than that in the vaginal delivery group. Conclusions Cesarean delivery is more prone to NRDS than vaginal delivery in different delivery modes, and there are differences in the image classification of children with NRDS in cesarean delivery. The image classification of children with NRDS in cesarean delivery is more severe than that in vaginal delivery, and the condition is more serious. Clinical attention should be paid to the risk of infants born by cesarean delivery and premature infants with NRDS.
2022, 43(10): 143-146.
doi: 10.12259/j.issn.2095-610X.S20221010
Abstract:
Objective To investigate HPV infection, subtype distribution, and age distribution in females in Kunming. Methods Flow fluorescence hybridization was used to detect HPV genotypes in 6091 female cervical exserted cells in Kunming Maternal and Child Health Hospital in 2021. Infection rate, gene subtypes and age distribution were obtained after classification and statistics. Results 1301 people wereinfected with HPV, the infection rate was 21.36%. Single infected persons accounted for the majority （78.94%）. In all positive specimens, the number of high-risk infection cases was more than that of low-risk infection cases. The highest infection rates were HPV52 （21.83%）, HPV16 （11.38%）, and HPV58 （10.76%）, respectively. Participants were divided into ≤20 years old group, 21 to 30 years old group, 31 to 40 years old group, 41 to 50 years old group, 51 to 60 years old grou, p and > 60 years old group according to age. The HPV infection rate was highest in the > 60 years old group, followed by the 21 to 30 years old group and the 51 to 60 years old group. Conclusions HPV infection in women in Kunming is mainly a hhigh-risk type. Elderly and people under 30 years old need to strengthen prevention and treatment education, and it is recommended that women of appropriate age receive multivalent cervical cancer vaccine as soon as possible.
2022, 43(10): 147-152.
doi: 10.12259/j.issn.2095-610X.S20221030
Abstract:
Objective To investigate the relationship between electrocardiographic frontal QRS-T angle, Tp-e/QTc ratio, and coronary slow flow phenomenon （CSFP） and evaluate the predictive value of frontal QRS-T angle and Tp-e/QTc ratio. Methods Seventy-three patients with CSFP diagnosed by coronary angiography in our hospital from September 2019 to December 2021 were selected as the study group, and 67 patients with the normal coronary flow in the same period were randomly selected as the control group. The clinical data, related ECG parameters, and laboratory indexes of the two groups were compared. Multivariate Logistic regression analysis was used to analyze the risk factors affecting the risk factors of CSFP. The receiver operating characteristic （ROC） curve was drawn to explore the predictive value of frontal QRS-T angle and Tp-e/QTc ratio. Results Compared with the control group, frontal QRS-T angle, Tp-e interval, Tp-e / Qt ratio, Tp-e / QTc ratio, BMI, Cr, RBC, Hb, and HCT were higher, and the two groups were statistically different （all P < 0.05）. In the multivariate Logistic regression analysis show that BMI （OR = 1.137, P = 0.014）、Cr （OR = 1.048, P = 0.004）, frontal QRS-T angle （OR = 1.020, P = 0.003）, Tp-e/QTc （IQR） grade OR = 1.960, P < 0.001） were independent risk factors for CSFP. ROC curve analysis displayed that the diagnostic cut-off value of frontal QRS-T angle was 45.5 degree, The area under the curve was 0.706, and the sensitivity and specificity were 56.2% and 80.1%, respectively; the diagnostic cut-off value of CSFP for Tp-Te/QTc ratio was 0.22, the area under the curve was 0.68, and the sensitivity and specificity were 66% and 66%, respectively. 69%. Conclusion Frontal QRS-T angle and Tp-e/QTc ratio were associated with CSFP, which had a certain potential predictive value for the occurrence of CSFP.
2022, 43(10): 153-162.
doi: 10.12259/j.issn.2095-610X.S20221020
Abstract:
Objective To investigate the genetic polymorphisms of 20 autosomal STR loci in Miao population from Wenshan and Honghe regions, and to explore their forensic values. Methods DNA was extracted with the Chelex-100 method. A total of 20 STR loci were amplified from 2209 healthy unrelated individuals of the Miao population from Wenshan and Honghe regions, Yunnan Province using the PowerPlex®21 System kit. The PCR products were separated by capillary electrophoresis on an ABI 3130Genetic Analyzer, and alleles were genotyped by GeneMapper ID （Version 3.2） software. Forensic parameters were calculated by Modified-Powerstats software, and the Hardy-Weinberg equilibrium （HWE） test was performed. The Fst values and Nei's genetic distance were calculated by Arlequin and Phylip software, respectively. Multidimensional scaling （MDS） analysis was performed with SPSS v24.0 statistical software and the neighbor-joining （N-J） phylogenetic tree was constructed using the MEGA v6.0 software. Results A total of 265 alleles and 1080 genotypes were observed. The allele frequency of the 20 STRs ranged from 0.0002 to 0.5718. No deviation from Hardy-Weinberg equilibrium （P > 0.05/20 = 0.0025） was observed. The cumulative discrimination power （CDP） and cumulative probability of exclusion （CPE） with 20 STRs were 0.999 999 999 999 999 999 999 932 674 151 and 0.999 999 935 545 335, respectively. Population comparison showed that Wenshan and Honghe Miao nationality was genetically closer to Yunnan Vietnamese. Conclusion These 23 STR loci are highly genetic polymorphic and informative in the Miao population from Wenshan and Honghe regions, Yunnan Province, which can be used for forensic identification and population genetic research.
2022, 43(10): 163-167.
doi: 10.12259/j.issn.2095-610X.S20221005
Abstract:
Chronic periapical periodontitis is a common oral disease secondary to dental pulp infection, which often leads to bone destruction in the apical region because of bacterial infection in the root canal. Because of the particularity of human individuals, in many cases, the dynamic changes of the pathogenesis of periapical tissue can not be directly studied, it is very important to select and establish an appropriate animal model. At present, the animal models commonly used to establish chronic periapical periodontitis mainly include monkeys, dogs, rats and miniature pigs. Among them, SD rats are often used to study the pathogenesis of periapical periodontitis because of their advantages of fast growth, easy culture, strong adaptability, low price, and easy feeding. This paper reviews the research on the animal model of chronic apical periodontitis in SD rats at home and abroad from the aspects of the basis, methods and evaluation methods after the establishment of the model, so as to provide a theoretical basis for the clinical study of the development mechanism and treatment of chronic apical periodontitis.
Chronic periapical periodontitis is a common oral disease secondary to dental pulp infection, which often leads to bone destruction in the apical region because of bacterial infection in the root canal. Because of the particularity of human individuals, in many cases, the dynamic changes of the pathogenesis of periapical tissue can not be directly studied, it is very important to select and establish an appropriate animal model. At present, the animal models commonly used to establish chronic periapical periodontitis mainly include monkeys, dogs, rats and miniature pigs. Among them, SD rats are often used to study the pathogenesis of periapical periodontitis because of their advantages of fast growth, easy culture, strong adaptability, low price, and easy feeding. This paper reviews the research on the animal model of chronic apical periodontitis in SD rats at home and abroad from the aspects of the basis, methods and evaluation methods after the establishment of the model, so as to provide a theoretical basis for the clinical study of the development mechanism and treatment of chronic apical periodontitis.
2022, 43(10): 168-177.
doi: 10.12259/j.issn.2095-610X.S20221003
Abstract:
Eight South Asian countries and eleven Southeast Asian countries along the "One Belt and One Road" are areas with high incidences of parasitic diseases. Due to the influence of geographical location, economic status, cultural background, and dietary habits, several human parasites such as Plasmodium spp, Leishmania spp, Toxoplasma gondii, Giardia lamblia, Blastocystis hominis, Entamoeba histolytica, Trichomonas vaginalis, schistosoma japonicum, Ascaris lumbricoides, Trichiruss trichiura, hookworm, strongyloides stercoralis, Enterobius vermicularis, tapeworms, and filaria are prevalent. Parasitic diseases are a serious public health problem in these regions. Analyzing the epidemic situation of parasitic diseases in South and Southeast Asian countries along the "One Belt and One Road", and formulating targeted measures of prevention and control are helpful for preventing the occurrence of cross-border infectious diseases, promoting close cooperation between countries, and completely eliminating parasitic diseases.
Eight South Asian countries and eleven Southeast Asian countries along the "One Belt and One Road" are areas with high incidences of parasitic diseases. Due to the influence of geographical location, economic status, cultural background, and dietary habits, several human parasites such as Plasmodium spp, Leishmania spp, Toxoplasma gondii, Giardia lamblia, Blastocystis hominis, Entamoeba histolytica, Trichomonas vaginalis, schistosoma japonicum, Ascaris lumbricoides, Trichiruss trichiura, hookworm, strongyloides stercoralis, Enterobius vermicularis, tapeworms, and filaria are prevalent. Parasitic diseases are a serious public health problem in these regions. Analyzing the epidemic situation of parasitic diseases in South and Southeast Asian countries along the "One Belt and One Road", and formulating targeted measures of prevention and control are helpful for preventing the occurrence of cross-border infectious diseases, promoting close cooperation between countries, and completely eliminating parasitic diseases.
2022, 43(10): 178-181.
doi: 10.12259/j.issn.2095-610X.S20221024
Abstract:
Objective To discover the role of “personality color analysis” in improving PBL teaching efficiency of Integration course. Methods Fifty students from 2017 innovative class of Kunming Medical University were selected as the control class, the students were grouped according to their student numbers.58 students from 2019 innovative class of were selected as the experimental class, and the students were grouped according to the “personality color analysis” theory. The scores, grades and differences between groups were compared. Results There was a significant difference （P < 0.05） in the scores of the two classes, and comparing the grade distribution of the two classes, the proportion of students in the experimental class （10%） with < 9 points were lower than that in the control class （18%）, and the proportion of students in the experimental class （40%） with 13-15 points was higher than that in the control class （14%）. Furthermore, there was no difference between groups in the experimental class, but there was a significant difference between groups in the control class. Conclusion Applying the theory of “personality color analysis” to the PBL of organ-system integration course reduces the gap between groups and improve the learning efficiency, which deserves further application and popularization in PBL.
2022, 43(10): 182-186.
doi: 10.12259/j.issn.2095-610X.S20221023
Abstract:
Objective To explore the application effect of micro-video teaching in lung physical examination of clinical medical students. Methods A total of 80 five-year undergraduates of 2016 and 2017 clinical departments of Kunming Medical University were randomly selected as the control group, and 82 five-year undergraduates of 2018 and 2019 clinical departments were randomly selected as the experimental group. The traditional teaching methods were used in the control group, and the teaching mode of combining micro video and traditional teaching was used in the experimental group. At the end of the course, the results of practical skills examination and questionnaire survey are used to evaluate the teaching effect. Results The test scores of the control group were lower than those of the experimental group （83.16±8.80）, and the difference was statistically significant （P < 0.05）. The results of questionnaire survey showed that the scores of intuitiveness and comprehensibility in the control group （8.09±1.94） were lower than those in the experimental group （8.96±1.36）. The mastery score of the control group （8.21±1.80） was lower than that of the experimental group （8.74±1.58）. The memorability score of the control group （4.60±0.89） was lower than that of the experimental group （8.41±1.85）. The score of exercise referability in the control group （7.76±2.03） was lower than that in the experimental group （9.05±1.62）. The timeliness score of teacher feedback in the control group （8.35±1.90） was lower than that in the experimental group （9.29±1.16）. The score of teacher feedback in the control group （8.49±1.69） was lower than that in the experimental group （9.26±1.30）, and there were statistically significant differences between the experimental group （micro video combined with traditional teaching group） and the control group （traditional teaching group） （P < 0.05）. At the same time, the students’ learning enthusiasm, learning efficiency and autonomous learning ability are improved and they have strong problem-solving ability. Conclusion The teaching mode of combining micro video with traditional teaching can improve the teaching effect and quality in clinical teaching, which is worth popularizing in clinical teaching.
2022, 43(10): 187-192.
doi: 10.12259/j.issn.2095-610X.S20221009
Abstract:
Objective To investigate the effect of 5 g salt reduction based on peereducation in the self-management of elderly hypertensive patients in the community, so as to control and reduce hypertension and improve their self-care ability in the community, enable elderly hypertensive patients to maintain a healthier lifestyle, as well as provide a basis for salt reduction prevention and treatment of hypertension in the elderly in the country. Methods In this study, a random number method was used to randomly divide 76 patients with high-normal blood pressure and grade 1 and 2 hypertension aged 60 ～ 75 years old in Dong Hua and Union Community of Kunming City, Yunnan Province into an experimental group and control group, 38 cases in each group. The control group received routine hypertension health education; based on community, and the experimental group received 5 g salt reduction intervention based on peer education on the basis of routine hypertension education strategy and follow-up intervention in the first and third months. The scores of blood pressure, health behavior scale （ HPLP-II ）, self-care ability scale （ ESCA ）, concise health status survey scale （ SF-36 ）, and salt cognition were compared between the two groups before and after the intervention. Results There was no statistically significant difference in the HPLP-II, ESCA, and SF-36 scale scores, salt awareness score, and hypertension between the two groups of elderly hypertensive patients before the intervention （P > 0.05）. after the intervention, the ESCA scale score was lower than that of the control group （P < 0.05）, the HPLP-II, SF-36 scale scores, and salt awareness scores were higher than those of the control group, and hypertension The control of hypertension was better than that of the control group （P < 0.05）. Conclusion By implementing a 5 g salt reduction intervention based on peer education for elderly hypertensive patients with normal high values and grades 1 and 2, it can effectively control and reduce the blood pressure values of elderly hypertensive patients, promote their awareness and rational intake of salt and their self-care, thereby developing healthy eating habits and improving their quality of life.
