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RSS2023 Vol. 44, No. 10
2023, 44(10): 1-9.
doi: 10.12259/j.issn.2095-610X.S20231016
Abstract:
Objective To construct stable transfected cell lines of non-small cell lung cancer A549 with overexpression and knockdown LncRNA RP11-521C20.3. Methods According to lncRNA RP11-521C20.3 gene sequence, primers were designed and amplified. The target ...
2023, 44(10): 10-17.
doi: 10.12259/j.issn.2095-610X.S20231021
Abstract:
Objective To explore the protective effect of butyric acid on intestinal epithelial barrier damage induced by TNF α. Methods CCK-8 cell viability assay was used to determine the optimal concentration and time of TNFα in exerting damaged effects on C...
2023, 44(10): 18-25.
doi: 10.12259/j.issn.2095-610X.S20231019
Abstract:
Objective To investigate the protective effects of different concentrations and durations of corilagin treatment on oxidative low-density lipoprotein （ox-LDL）-induced damage in human umbilical vein endothelial cells （HUVECs）, as well as its effects o...
2023, 44(10): 26-32.
doi: 10.12259/j.issn.2095-610X.S20231004
Abstract:
Objective To explore the effect of exercise rehabilitation on cardiac fibrosis and function in rats with myocardial infarction. Methods Twenty-four male SD rats were randomly divided into four groups: MI-E group, MI-Sed group, Sham-E group and Sham-...
2023, 44(10): 33-38.
doi: 10.12259/j.issn.2095-610X.S20231009
Abstract:
Objective To explore the antinociception of acupotomology in rats with sciatic nerve ligation, so as to provide experimental basis for the clinical application and promotion of needle-knife therapy on neuralgia. Methods The hot-plate test was used t...
2023, 44(10): 39-46.
doi: 10.12259/j.issn.2095-610X.S20231018
Abstract:
Objective To investigate the effects and preliminary mechanisms of the extract CII-3 and defatted ointment of Periplaneta americana on autophagy, invasion and metastasis of drug-resistant hepatocellular carcinoma cell line BEL-7402/5-FU. Methods The...
2023, 44(10): 47-54.
doi: 10.12259/j.issn.2095-610X.S20231028
Abstract:
Objective To investigate the effect of hsa-let-7a-5p on the proliferation and apoptosis of periodontal ligament stem cells （PDLSCs）. Methods let-7a-5p mimics, mimics nc, let-7a-5p inhibitor, and inhibitor nc were transfected into PDLSCs, and transfe...
2023, 44(10): 55-59.
doi: 10.12259/j.issn.2095-610X.S20231010
Abstract:
Objective To study the spatiotemporal expression profiles of miR-25 in the Chinese perch（Siniperca chuatsi）, to predict its targeting core Circadian clock genes, and to explore its possible biological significance. Methods Real-time fluorescence qua...
2023, 44(10): 60-66.
doi: 10.12259/j.issn.2095-610X.S20231026
Abstract:
Objective To investigate the effect and regulatory mechanism of metformin on autophagy and cell proliferation of vascular smooth muscle cells （VSMCs） cultured in high glucose in mice aortic. Methods Western blot was used to detect the changes in mic...
2023, 44(10): 67-76.
doi: 10.12259/j.issn.2095-610X.S20231017
Abstract:
Objective To explore the synergizing effect of histone methyltransferase enhancer of Zeke 2 （EZH2） inhibitors on Gemcitabine and Cisplatin （GC） chemotherapy regimen in bladder cancer treatment. Methods Firstly, EZH2 siRNA knockdown lines were const...
2023, 44(10): 77-82.
doi: 10.12259/j.issn.2095-610X.S20231022
Abstract:
Objective To investigate the therapeutic effects of probiotics containing Bifidobacterium on a mouse model of non-alcoholic fatty liver disease （NAFLD） and its impact on the gut microbiota. Methods C57BL/6J mice were divided into three experimental ...
2023, 44(10): 83-91.
doi: 10.12259/j.issn.2095-610X.S20231023
Abstract:
Objective To explore the mechanisms of miR-196b affecting the progression of lung adenocarcinoma （LUAD）. Methods The expression level of miR-196b in LUAD tissues was analyzed through TCGA database. TargetScan was used to predict its downstream targ...
2023, 44(10): 92-99.
doi: 10.12259/j.issn.2095-610X.S20231024
Abstract:
Objective To investigate the effect of pristimerin on the proliferation ability of human oral squamous cell carcinoma cells CAL-27 and its related mechanisms. Methods CCK-8 assay was performed to determine the proliferative bioactivity of CAL-27 cel...
2023, 44(10): 100-106.
doi: 10.12259/j.issn.2095-610X.S20231011
Abstract:
Objective To understand the occurrence of depression symptoms and its influencing factors among youth in eight indigenous ethnic minority groups in Yunnan Province, in order to provide a basis for promoting the psychological health of different ethni...
2023, 44(10): 107-113.
doi: 10.12259/j.issn.2095-610X.S20231003
Abstract:
Objective To investigate the correlations of age, diseases and blood pression （BP）. Methods A retrospective study was conducted to include 82 782 patients who underwent physical examination in the physical examination department of Hudong Hospital ...
2023, 44(10): 114-121.
doi: 10.12259/j.issn.2095-610X.S20231005
Abstract:
Objective To study the genetic variation characteristics of HPV56 E6, E7 and L1 in patients with precancerous lesions infected with HPV56, and to obtain the molecular epidemiological data of HPV56 in patients with precancerous lesions of cervical can...
2023, 44(10): 122-128.
doi: 10.12259/j.issn.2095-610X.S20231012
Abstract:
Objective To understand the changes in peripheral blood lymphocyte subsets in individuals who have migrated to high-altitude low-oxygen environments and returned to lower altitude areas, and to explore the impact of high-altitude deadaptation on the ...
2023, 44(10): 129-133.
doi: 10.12259/j.issn.2095-610X.S20231001
Abstract:
Objective To investigate the basic status of refractive development in children aged 6～36 months and analyze the related influencing factors. Methods A total of 562 children aged 6～36 months were selected to detect the diopter of both eyes without c...
2023, 44(10): 134-139.
doi: 10.12259/j.issn.2095-610X.S20231027
Abstract:
Objective To establish a convolutional neural network model for ultrasound diagnosis of ovarian tumors and explore its value in distinguishing between benign and malignant ovarian tumors. Methods A total of 400 ovarian tumor ultrasound images, includ...
2023, 44(10): 140-148.
doi: 10.12259/j.issn.2095-610X.S20231006
Abstract:
Objective To explore the differences of maternal placental microbiota DNA between gestational diabetes mellitus （GDM） and normal full-term infants, and provide reference data for further research. Methods A total of 30 cases of full-term infants’ pl...
2023, 44(10): 149-154.
doi: 10.12259/j.issn.2095-610X.S20231025
Abstract:
Objective To evaluate the accuracy of digital models obtained using the iTero Element 1 intraoral scanner and traditional plaster models for measurements in mild to moderate crowding. Methods 37 patients who sought treatment at the Qinxing Road Clin...
2023, 44(10): 155-160.
doi: 10.12259/j.issn.2095-610X.S20231015
Abstract:
Objective To explore the gene variation profile of Gilbert syndrome in Kunming, and to provide basic data for the mechanisms of Gilbert syndrome. Methods 357 children with Gilbert syndrome in Kunming were analyzed by high-throughput sequencing and ...
2023, 44(10): 161-167.
doi: 10.12259/j.issn.2095-610X.S20231014
Abstract:
Objective To explore the association between single nucleotide polymorphisms of TMTC1 gene and schizophrenia. Methods A total of 207 patients with schizophrenia and 186 healthy controls were included in the study. SNPscanTM multiplex SNP genotyping ...
2023, 44(10): 168-179.
doi: 10.12259/j.issn.2095-610X.S20231007
Abstract:
Signal pathways including MAPK, PI3K/AKT, AMPK, TGF-β superfamily, Wnt, Hippo, NF-κB, Notch, JAK/STAT, Hedgehog, integrin, OPG/RANKL/RANK, HIF, etc. are all related to osteogenic differentiation and play a certain regulatory role in the process of bo...
Signal pathways including MAPK, PI3K/AKT, AMPK, TGF-β superfamily, Wnt, Hippo, NF-κB, Notch, JAK/STAT, Hedgehog, integrin, OPG/RANKL/RANK, HIF, etc. are all related to osteogenic differentiation and play a certain regulatory role in the process of bo...
2023, 44(10): 180-188.
doi: 10.12259/j.issn.2095-610X.S20231020
Abstract:
Triple-positive breast cancer （TPBC） is a special type of breast cancer characterized by the co-expression of hormone receptors （HR+） and human epidermal growth factor receptor 2 （HER2+）. Due to the regulation of the HR signaling pathway and the HER2...
Triple-positive breast cancer （TPBC） is a special type of breast cancer characterized by the co-expression of hormone receptors （HR+） and human epidermal growth factor receptor 2 （HER2+）. Due to the regulation of the HR signaling pathway and the HER2...
2023, 44(10): 189-195.
doi: 10.12259/j.issn.2095-610X.S20231013
Abstract:
Since penicillin was used to treat bacterial infectious diseases in 1928, antibiotics have saved countless patients’ lives. However, with the increase of bacterial drug resistance, the clinical treatment of bacterial infectious diseases has become in...
Since penicillin was used to treat bacterial infectious diseases in 1928, antibiotics have saved countless patients’ lives. However, with the increase of bacterial drug resistance, the clinical treatment of bacterial infectious diseases has become in...
2023, 44(10): 196-201.
doi: 10.12259/j.issn.2095-610X.S20231008
Abstract:
Objective To evaluate the impact of research-based learning （RBL）, a new teaching method, on the critical thinking ability of medical postgraduates in order to enhance their critical thinking skills. Methods A total of 330 postgraduate students majo...
2023, 44(10): 202-206.
doi: 10.12259/j.issn.2095-610X.S20231002
Abstract:
Objective To explore the application effect of dynamic monitoring of lung ultrasound in precision nursing care of severe neonatal pneumonia with pulmonary consolidation. Methods 60 newborns diagnosed with severe pneumonia in the Neonatal Intensive ...
