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RSS2023 Vol. 44, No. 5
2023, 44(5): 1-5.
doi: 10.12259/j.issn.2095-610X.S20230528
Abstract:
Objective To study the chemical constituents from the herbs of Pleione bulbocodioides and find theirs antitumor bioactive compounds. Methods The constituents were extracted by ethyl acetate and isolated by CC and Sephadex LH-20. Their structures were identified by spectroscopic analysis （1H NMR, 13CNMR and EIMS）. The antitumor activity of the constituents was studied by MTT assay in vitro. Results Twelve compouds were obtained and identified as 4-methoxy-2, 7-dihydroxyphenan- -threne （1）, （8R）-4, 5′-dihydroxy-8-hydroxymehtyl-3′-methoxydeoxybenzoin （2）, 4, 7-dihydroxy-2-methoxy-9, 10-dihydrophenanthrene （3） 2-hydroxy-4, 7-dimethoxy-phenanthrene （4） Ursolic acid （5） β-sitosterol （6） 2-hydroxy-4, 7-dimethoxy-9, 10-dihydrophenanthrene （7） 4- （Benzofuranyl）-phenol （8） Flavanthrinin （9） Coelonin （10） CH3 （CH2）27OH （11） and 4-dimethoxy benzaldehyde （12）. The results of anti-tumor activity test of compounds 1～5 showed that 2 expressed good inhibitory activity on SKOV-3 cell line. Conclusion Compounds 2, 5, 8, 11 have been isolated from the herbs for the first time and IC50 of 2 on SKOV-3 cell line is 12.79 μM.
2023, 44(5): 6-11.
doi: 10.12259/j.issn.2095-610X.S20230506
Abstract:
Objective The aim of this study is to investigate the effect of Diallyl trisulfide （DATS） on placental angiogenesis in mice with unexplained recurrent spontaneous abortion （URSA） and to explore its mechanism based on the vascular endothelial growth factor （VEGF）/vascular endothelial growth factor receptor-2 （VEGFR-2） signaling pathway. Methods Sixteen URSA mice selected were randomly divided into the control group and the experimental group, with 8 mice in each group. The experimental group was given 98% pure DATS （200 μL suspension prepared according to 50 mg/（kg·d) by gavage. The rats in the control group were fed conventionally and given 200 μL PBS solution by gavage. After 18 days of continuous administration, Hydrogen sulfide （H2S） content in the placental tissue was detected by spectrophotometer, and the relative expression of CD31, VEGFA and VEGFR2 mRNA in the placental tissue was detected by reverse transcriptase-polymerase chain reaction （RT-qPCR）. The protein expression levels of VEGFA and VEGFR2 in placenta were detected by Western blot. Results The spectrophotometer detection showed that the H2S level in the experimental group （777.4922±72.9759） was significantly higher than that in the control group （529.1824±99.7489） （P < 0.05）; The RT-qPCR results showed that the relative expression of CD31 in the experimental group （0.0042±0.0006） was significantly higher than that in the control group （0.0020±0.0004） （P < 0.05）, the relative expression of VEGFA in the experimental group （0.7073±0.0677） was significantly higher than that in the control group （0.5200±0.0946）（P < 0.05）, and the relative expression of VEGFR2 in the experimental group （0.7304±0.1262） was significantly higher than that in the control group （0.3984±0.047） （P < 0.05）; Western blot results showed that DATS significantly increased the protein expression levels of VEGFA and VEGFR2 in placental tissue （P < 0.05）. Conclusion DATS can significantly increase the level of H2S in URSA placental tissue and the relative expression levels of angiogenic factors CD31, VEGFA, and VEGFR2 in placental tissue. Through the VEGF signal axis, it can improve the formation of placental blood vessels in URSA mice. The relevant research results will provide scientific basis and reference value for the clinical application of DATS.
2023, 44(5): 12-18.
doi: 10.12259/j.issn.2095-610X.S20230530
Abstract:
Objective To investigate the effects of Osteoking combined with antibiotic cocktail on insulin resistance and gut microbiota in db/db mice. Methods The wild mice were used as the control group, while db/db mice were randomly divided into the model group, Osteoking group （OK）, antibiotic group （ABX）, and Osteoking combined with antibiotic cocktail group （OA）. After the intragastric administration for 11 weeks, the following indices were investigated: body weight, fasting blood-glucose （FBG）, serum insulin and the changes of intestinal microflora by 16S rDNA sequencing technology in mice. Results Compared with the model group, levels of FBG and HOMA- IR were significantly decreased in OK, ABX, AO groups （P < 0.05）. And the shannon、simpson and pielou_e index of gut microbiota were significantly decreased in ABX group （ P < 0.05）, while they were increased in OK group （ P < 0.05）. The shannon and pielou_e index were increased in AO group （ P < 0.05）. At the phylum level, the relative abundance （RA） of Firmicutes （ P < 0.05） in OK group, RA of Proteobacteria in ABX （ P < 0.01）and AO groups （ P < 0.01） were significantly increased; while RA of Bacteroidota was significantly decreased in OK （ P < 0.05）, ABX （ P < 0.01）, AO （ P < 0.01）groups. At the family level, RA of Lachnospiraceae （ P < 0.05） in OK group, Enterobacteriaceae, Sutterellaceae in ABX and AO groups （ P <0.01）and Tannerellaceae in AO groups （ P < 0.01） were significantly increased; while RA of Muribaculaceae in OK group was significantly decreased （ P < 0.05）. The following gut microbiota were riched: Proteobacteria in ABX group, Lachnospiraceae NK4A136 group, Lactobacillus, Desulfobacteraceae and Prevotellaceae in OK group, Parabacteroides gordonii, Akkermansia, Morganellaceae and Alphaproteobacteria in AO group. Conclusion Osteoking, antibiotic cocktail alone or in combination have the effects of improving the insulin resistance in db/db mice. And Osteoking can improve the intestinal microflora imbalance induced by antibiotic cocktail.
2023, 44(5): 19-25.
doi: 10.12259/j.issn.2095-610X.S20230505
Abstract:
Objective To understand and analyze the incidence of premature delivery and its influencing factors in Qujing city, Yunnan province, so as to provide the scientific basis for the early intervention. Methods Based on Qujing Maternal and Child Health Hospital, we selected the pregnant women who met the inclusion criteria from September 1, 2020 to October 31, 2021 as the research objective, followed up the basic situation and delivery outcome of pregnant women, and analyzed the epidemiological characteristics and influencing factors of premature delivery by chi-square test and logistic regression model. Results Among 7302 pregnant women, 477 cases were premature, with an incidence of 6.53%. Among them, late preterm labor, middle preterm labor and early preterm labor accounted for 77.15%, 12.79% and 10.06% respectively. Multivariate analysis showed that the risk factors of premature delivery were: low education level （i.e. primary school or below, junior high school and senior high school）, pregnancy ≥3 times, parity ≥3 times, placenta previa, twin or multiple pregnancy, history of cesarean section, history of abortion, number of check-ups ≤8 times, gestational hypertension, gestational diabetes, premature rupture of membranes, fetal or uterine malformation （all P-value < 0.05）. Conclusion The incidence of premature delivery is high, which may be influenced by the education level of pregnant women, placenta previa during pregnancy, twin or multiple pregnancies, the number of prenatal checkups, pregnancy-induced hypertension, gestational diabetes, premature rupture of membranes, fetal or uterine malformation, and previous history of cesarean section and abortion. Therefore, we should strengthen the ability to accurately identify the risk factors of premature delivery at an early stage, so as to achieve the early detection, early prevention and early treatment, reduce its impact on mother and fetus, and promote the health of mother and baby.
2023, 44(5): 26-31.
doi: 10.12259/j.issn.2095-610X.S20230509
Abstract:
Objective To study the epidemiological characteristics of neonatal rotavirus （RV） infection, and to provide the guidance for the treatment and prevention of clinical diseases and the formulation of prevention and treatment strategies for RV infection in hospitals. Methods The clinical data of newborns admitted to a 3A children’s hospital in Yunnan Province were retrospectively collected from January 2020 to December 2022. The RV antigen in feces was detected by group A rotavirus detection reagent （colloidal gold method）, and the clinical and epidemiological data of neonatal RV infection were compared and analyzed by SPSS 26.0. Results There were 261 cases of RV infection in 16761 newborn patients, and the infection rate was 1.56%, of which the infection rates in 2020-2022 were 1.97% （124/6298）, 1.01% （57/5660） and 1.67% （80/4803） respectively. The RV infection rate of male children was 1.39%, while that of female children was 1.79%, and the difference between them was statistically significant （P < 0.05）. The infection rate of rotavirus in newborns aged ≤7 days was significantly lower than that in newborns aged 8～28 days, and the difference was statistically significant （P < 0.05）. From 2020 to 2022, the neonatal RV infection rates in spring, summer, autumn and winter were 2.64% （102/3866）, 1.58% （71/4502）, 0.63% （28/4450） and 1.52% （60/3943） respectively, and the difference was statistically significant （P < 0.05）.The top four states （cities） of neonatal RV infection rates in different origin areas were 7.14% （3/42） in Diqing, 2.82% （2/71） in Pu’er, 2.31% （8/346） in Honghe and 2.19% （3/137） in Wenshan. Community infection accounted for 62.07% （162/261） and hospital infection accounted for 37.93% （99/261）. There were 22.22% （58/261） children with asymptomatic infection, and 77.78% （203/261） children with RV infection-related clinical symptoms such as diarrhea, fever and vomiting. There are differences between the different nationalities （χ2 = 7.945, P < 0.05） and different RV antigen test results （χ2 = 5.007, P < 0.05） in the presence or absence of symptoms of RV infection in newborns. Conclusion RV infection rate of hospitalized newborns is generally low, especially in late newborns, and the infection rate is higher in areas with relatively backward economic level. It is suggested that more attention should be paid to RV infection in newborns, health education for caregivers should be strengthened and comprehensive prevention measures should be formulated to control the neonatal rotavirus infection from the source.
2023, 44(5): 32-37.
doi: 10.12259/j.issn.2095-610X.S20230513
Abstract:
Objective To explore the alcohol dependence state among Dulong ethnic residents in Yunnan province and its influencing factors so as to delay the increase in the prevalence. Methods With the use of the cross-sectional research methods, a random cluster sampling survey was conducted on the Dulong ethnic group residents （aged 18-65） in Yunnan Province for on-site questionnaire survey. Results Among 456 people surveyed, 199 had the drinking behavior, and the drinking rate was 43.64% （199/456）. 87 people were found to have alcohol dependence and the incidence of alcohol dependence was 19.08% （87/456）. Among the residents drinking alcohol, the incidence of alcohol dependence was 43.72% （87/199）, and the severity of alcohol dependence in residents with the alcohol dependence was mainly mild. Spearman correlation analysis showed that the alcohol dependence of Dulong residents was positively correlated with drinking frequency （rs = 0.749, P < 0.001）. Through Logistic regression analysis, it was found that age, occupation and alcohol consumption were the main influencing factors of alcohol dependence （P < 0.05）. Conclusion The incidence of alcohol dependence among Dulong residents in Yunnan is high. Among the residents who drink alcohol, the incidence of alcohol dependence is high. The severity of alcohol dependence is mainly light. Relevant intervention should be carried out as soon as possible for residents with alcohol dependence.
2023, 44(5): 38-42.
doi: 10.12259/j.issn.2095-610X.S20230525
Abstract:
Objective To investigate the epidemiological characteristics of male patients with the first-ever alcohol-induced psychiatric disorders. Methods A retrospective survey was conducted and the medical records of inpatients first diagnosed with alcohol-induced psychosis/alcohol-related disorder （ICD-10 diagnostic criteria） were analyzed at the Second People’s Hospital of Honghe Hani and Yi Autonomous Prefecture from July 2010 to July 2022. Results A total of 668 first-episode male patients with alcohol induced mental disorders were admitted during the 12-year period, with an average age of （46.18±10.07） years. Among them, the distribution of ethnicity was most common among Hani, Yi and Han, the distribution of education was most common among the illiterates and elementary schools, the distribution of marital status was most common among the married, the distribution of occupation was most common among farmers, and the first visit was most common in Yuanyang County. The mean age of the first alcohol consumption was higher among Hani patients than that among Han and Yi patients （P < 0.05）, but there was no statistical difference in the mean age of alcohol dependence and psychotic symptoms and the structure of hallucinations and delusions type at admission between the ethnic groups （ P > 0.05）; Age at the first alcohol consumption was significantly and positively correlated with the age at the onset of alcohol dependence and the age at the onset of psychotic symptoms, respectively, and the age at the onset of alcohol dependence was significantly and positively correlated with the age at the onset of psychotic symptoms. Conclusion The earlier the age of drinking, the earlier the age at which alcohol dependence and mental symptoms occur; The earlier the age at which alcohol dependence occurs, the earlier the age at which mental symptoms occur. Therefore, alcohol induced mental disorders are increasingly becoming a serious public health problem, and early intervention and enhanced health education are advocated.
2023, 44(5): 43-46.
doi: 10.12259/j.issn.2095-610X.S20230521
Abstract:
Objective To analyse the drinking water testing results in Shizhong district, Zaozhuang City from 2019 to 2022. Method 859 standard samples of drinking water in Shizhong District of Zaozhuang City from 2019 to 2022 were selected as the subjects. According to the “Standard Testing Method for Drinking Water”, common microorganisms in the collected samples were determined, and the determination results were judged based on relevant standards. Result Among the 859 samples of drinking water, 802 were found to be qualified, with an overall qualification rate of 93.36%. The qualification rate of total bacterial count in microbial indicators was 96.04% （with the lowest qualification rate of microbial coliform colonies was 95.11%）; The qualification rate for nitrate, nitrite, ammonia nitrogen, sulfate, chloride, soluble total solid, arsenic, cadmium, copper, zinc, mercury, selenium, and lead detection was 100.0%; There was a statistical difference in the qualified rate of water samples between 2019 and 2022 （χ2 = 7.781, P = 0.005）; There was statistical significance in the qualification rate of factory water samples, peripheral water samples, and source water samples; The qualification rate of peripheral water samples was the lowest, and the qualification rate of factory water samples was the highest; There was a statistical difference in the qualification rate between municipal water plants and town level water plants （χ2 = 35.437, P = 0.000）, indicating that the qualification rate of municipal water plants was higher than that of township water plants. Conclusion There are many problems with the hygiene status of drinking water in Shizhong District of Zaozhuang City from 2019 to 2022, with microbiological indicators exceeding the standard. Relevant departments should take intervention measures, strengthen water engineering management, and improve water quality in a timely manner to ensure safe drinking water for residents.
2023, 44(5): 47-52.
doi: 10.12259/j.issn.2095-610X.S20230529
Abstract:
Objective To investigate the expression characteristics of COX2 and CDX2 in the development of laryngeal squamous cell carcinoma and their relationship with the prognosis in patients with laryngeal squamous cell carcinoma. Methods The expression characteristics of COX2 and CDX2 in laryngeal polyps, laryngeal papilloma and laryngeal squamous cell carcinoma were detected at their protein levels by immunohistochemistry （IHC） and image analysis （IA） techniques in laryngeal squamous cell carcinoma. Twenty-five cases with laryngeal squamous cell carcinoma were followed up to understand the patient prognostic effects of COX2 and CDX2. Results 1. The expression of COX2 in laryngeal squamous cell carcinoma was lower than that of laryngeal polyps （P = 0.004） and laryngeal papilloma （P = 0.001）. The expression of COX2 in T3-T4 stage laryngeal squamous cell carcinoma was higher than that in T1-T2 stage, and the difference was statistically significant （P = 0.001）. The expression of COX2 in laryngeal squamous cell carcinoma with lymph node metastasis was higher than that in laryngeal squamous cell carcinoma without lymph node metastasis, the expression of COX2 increased gradually in low, medium and high differentiated laryngeal squamous cell carcinoma, but the differences were not statistically significant （P > 0.05）. 2. The expressions of CDX2 protein was weakly positive in laryngeal polyp and laryngeal papilloma, and the expressions of CDX2 protein was negative in laryngeal squamous cell carcinoma, and the difference was not statistically significant （ P > 0.05）. 3. Positive or negative expression of COX2 protein had no effect on the prognosis of patients. Conclusion 1. Low expression of COX2 may be associated with the development of laryngeal squamous cell carcinoma, but cannot predict the prognosis in patients with laryngeal squamous cell carcinoma. 2. CDX2 may not be a risk factor for laryngeal squamous cell carcinoma.
2023, 44(5): 53-59.
doi: 10.12259/j.issn.2095-610X.S20230526
Abstract:
Objective To develop and validate a risk prediction model for severe DPN in patients with type 2 diabetes using glucose variability parameters, and to provide the evidence for the prevention and treatment of diabetic chronic complications. Methods The medical records from 323 inpatients with T2DM who met the inclusion criteria were collected in the First Affiliated Hospital of Kunming Medical University from April 2019 to May 2020. Based on the professional sensory threshold measured, patients were divided into none-sever DPN group and severe DPN group. Lasso regression model was used to select risk factors. A risk prediction models for severe DPN was established and shown as a nomogram. ROC curve, calibration curve and decision curve analysis was used to validate the model. Results Age, smoking, dyslipidemia, HbA1c and TIR were significant predictors of severe DPN in type 2 diabetes patients, and TIR was an independent risk factor for severe DPN. An assessing model discrimination was established by using ROC curves with AUC = 0.653 （95%CI = 0.592 - 0.715, P < 0.05）. The Hosmer-Lemeshow test was used to determine the model fit with P value 0.074. Conclusion TIR can be a significant predictor of the severe DPN in patients with T2DM, and the clinical prediction model established on the basis of TIR has the fair accuracy. It is recommended to actively intervene in the patients with a risk of severe DPN greater than 20% （score > 250） evaluated by the model.
2023, 44(5): 60-65.
doi: 10.12259/j.issn.2095-610X.S20230527
Abstract:
Objective To investigate the clinical efficacy of SGLT2 inhibitors in delaying the progression of diabetic nephropathy based on "real world" clinical data. Methods A retrospective analysis was performed on 98 patients with diabetes who were treated with SGLT2 inhibitors from the MMC database of the Second Affiliated Hospital of Kunming Medical University from January 2019 to December 2022. The baseline data of treatment with SGLT2 inhibitors were taken as the baseline and the data of 6 months of treatment were collected. （1） General data [age, sex, height, BMI, systolic blood pressure, diastolic blood pressure] were compared between baseline and 6 months of the treatment; （2） Indexes of glucose metabolism [blood glucose, glycosylated hemoglobin （HbA1c）]; （3） Liver function indexes [AST, ALT, alkaline phosphatase, YGT, ALB]; （4） Lipid indexes [total cholesterol （TC）, triglyceride （TG）, high density lipoprotein （HDLc）, low density lipoprotein （LDLc）]; （5） Renal function indicators [urinary creatinine, urinary microalbumin, urinary microalbumin/creatinine （UACR）, urea nitrogen （BUN）, uric acid （UA）, creatinine （Cr）]. Following the "6 month interval" method, 98 patients with relatively complete data admitted to the hospital from January 2019 to December 2022 were divided into baseline group （98 cases, initial use of SGLT2 inhibitor） and 6 month group （98 cases, SGLT2 inhibitor treatment for 6 months）. Results The baseline age of the patients included in this study was 52.85±8.98 years old, and the baseline height was 167.62±8.04cm, including 72 males, accounting for 73.47%, and 26 females, accounting for 26.53%.After the 6 months of treatment: （1） The BMI, systolic blood pressure and diastolic blood pressure of the patients were lower than the baseline. There were significant differences in diastolic blood pressure （P < 0.05）. （2） The indexes of glucose metabolism, blood glucose and HbA1c were lower than the baseline, and the differences between the two indexes were statistically significant （P < 0.05）. （3） The liver function indexes AST, ALT, ALP, YGT and ALB were lower than the baseline, and the difference in ALT was statistically significant （P < 0.05）. （4） Lipid indexes TC, TG and LDLc were lower than the baseline, while HDLc was higher than the baseline, and the difference between TG and HDLc indexes was statistically significant （P < 0.05）. （5） The renal function indexes as urinary microalbumin, urinary microalbumin/creatinine （UACR） and urea nitrogen （BUN） were lower than the baseline, urinary creatinine, uric acid （UA） and creatinine （Cr） were higher than the baseline, and the differences in urinary microalbumin/creatinine （UACR） indexes were statistically significant （P<0.05）. Adverse reactions: In this study, no hypoglycemia, severe gastrointestinal reaction, urinary tract infection, lower limb wound injury or amputation were observed between the two groups. Conclusion SGLT2 inhibitors have the effects of lowering blood pressure, reducing body massindex controlling blood sugar level, regulating lipid metabolism, improving the renal function and reducing urinary protein in diabetic nephropathy patients. It can delay the progression of diabetic nephropathy and has high clinical safety.
2023, 44(5): 66-71.
doi: 10.12259/j.issn.2095-610X.S20230504
Abstract:
Objective To investigate the clinical characteristics and gene mutations of 2 patients in 2 families of autosomal recessive early-onset Parkinson’s disease （AREP）. Methods Clinical data and gene mutation analysis were performed on 2 patients from 2 Chinese Han families. Target capture and high-throughput sequencing were used to screen genes related to Parkinson’s disease （PD）, tremor, spinocerebellar ataxia, and dystonia; Multiple ligation-dependent probe amplification （MLPA） was used to detect the rearrangement and large deletion mutations of SNCA, LRRK2, PARK2, PINK1, PARK7, ATP13A2, UCHL1, GCH1 gene exons. Results 2 patients with clinically confirmed PD showed the obvious clinical and genetic heterogeneity. Gene detection found that there were two mutations in the PRKN gene exon 2 heterozygous deletion mutation and c.619G > T/p.Glu207Ter * heterozygous mutation in the patient of family 1. The compound heterozygous mutation was pedigree cosegregated in the family. The patients of pedigree 2 had homozygous deletion mutation in exon 3-4 of PRKN gene, and had heterozygous mutation in LRRK2 gene c.4827+6T > A, and heterozygous mutation in PINK1 gene c.1474C > T/p.Arg492*; Bioinformatics analysis found that the c.4827+6T > A mutation of LRRK2 gene may lead to its shear change. Conclusion The clinical manifestations and gene mutations of early-onset Parkinson’s disease caused by PRKN gene mutations are diverse; AREP patients may have multiple PD gene pathogenic mutations at the same time, and their clinical onset age is earlier, the symptoms are more severe and complex, and the disease progresses are faster.
2023, 44(5): 72-80.
doi: 10.12259/j.issn.2095-610X.S20230520
Abstract:
Objective To study the association of CaSR gene polymorphism with calcium nephrolithiasis and hypercalciuria in Chinese Dai population. Methods The Dai patients with calcium containing renal calculi in Dehong Prefecture, Yunnan Province, China were selected as the experimental group, and the healthy Dai people in Dehong Prefecture, Yunnan Province were taken as the control group. The venous blood was drawn for the determination of the three SNP loci rs7652589, rs1801725, and rs1042636 of the CaSR gene. The subjects in the stone group were tested for 24-hour urine calcium value, and the gene correlation was analyzed. Results There was a statistical difference between the homozygous AA gene and AA+GG gene at rs7652589 locus in the occurrence of calcium nephrolithiasis （P < 0.05）. The 24 h urine calcium value of the GG genotype with rs1042636 locus was significantly different from that of the AA based and AG genotype （P < 0.05）. There was a significant difference in 24 h urine calcium between those with GG genotype at rs7652589 locus and those with AA and AG genotype （P < 0.05） Conclusion The rs7652589 polymorphism on the CaSR gene in the Dai population in China is related to the occurrence of calcium nephrolithiasis and hypercalciuria. The GG gene at rs1042636 is related to the occurrence of hypercalciuria in the Dai population. The GG gene at rs7652589 may be a candidate gene for the occurrence of hypercalciuria leading to calcium nephrolithiasis in this ethnic group.
2023, 44(5): 81-89.
doi: 10.12259/j.issn.2095-610X.S20230501
Abstract:
Objective To classify the meibomian gland dysfunction （MGD） with the use of in vivo confocal microscopy （IVCM）, and to compare optimal pulse technology （OPT） combined with meibomian gland expression （MGX） in the treatment of different types of MGD-related dry eyes. Methods A total of 60 patients （60 eyes） with dry eyes associated with MGD were selected from November 2021 to October 2022 in Outpatient clinic of our hospital.The meibomian glands were examined by IVCM and divided into three types in the stage of increased severity: obstructive type （n = 29）, inflammatory type （n = 18） and fibrotic type （n = 13）. All patients were treated with OPT combined with MGX once every 2 weeks. The ocular surface disease index （OSDI） score, Noninvasive break-up time （NIBUT）, corneal fluorescein staining （CFS）, lipid layer thickness （LLT） and meibomian gland yielding secretion score （MGYSS） were observed and recorded before treatment, 1 month and 3 months after treatment. Results （1） OSDI score: The OSDI score of the three groups decreased 1 month and 3 months after treatment, and the OSDI score of obstructive type and inflammatory type decreased continuously 1 month after treatment compared with 3 months after treatment, the decrease of OSDI score of obstructive type was better than that of inflammatory type 1 months after treatment, and the decrease of OSDI score of obstructive type was better than that of inflammatory type and fibrotic type 3 months after treatment （P < 0.05）; （2） BUT: BUT of the three groups increased 1 month and 3 months after treatment, and the BUT of obstructive type increased continuously 1 month after treatment compared with 3 months after treatment, and the increase of BUT of obstructive type was better than that of inflammatory type and fibrotic type 1 month and 3 months after treatment （P < 0.05）; （3） CFS score:The CFS score of obstructive type decreased 1 month and 3 months after treatment, and the CFS score of inflammatory type and fibrotic type decreased 3 months after treatment, and the difference was statistically significant （P < 0.05）, There was no significant difference among the three types after treatment （P > 0.05）; （4） LLT: The LLT of obstructive type increased 1 month and 3 months after treatment, and the difference was statistically significant （P < 0.05）, There was no significant difference between inflammatory type and fibrotic type before and after treatment （P > 0.05）, There was no significant difference among the three types after treatment （P > 0.05）; （5） MGYSS:MGYSS of the three groups decreased 1 month and 3 months after treatment, and MGYSS decreased continuously in 1 month and 3 months after treatment in the three groups. the decrease of MGYSS in the obstructive type was better than that in the inflammatory type and fibrotic type （P < 0.05）; （6） According to IVCM classification, the stage of increased severity of MGD was positively correlated with the score of loss of meibomian gland （rs = 0.474, P < 0.001）. Conclusion （1） According to the classification of MGD by ICVM, it can be divided into three types in the stage of increased severity: obstruction type, inflammatory type and fibrotic type. IVCM is a new classification method for MGD, and according to IVCM classification, the stage of increased severity of MGD was positively correlated with the score of loss of meibomian gland; （2） On the basis of IVCM's classification of MGD, Treatment of different types of MGD-related dry eyes with OPT combined with MGX, The effect of obstruction type treatment is better.
2023, 44(5): 90-94.
doi: 10.12259/j.issn.2095-610X.S20230516
Abstract:
Objective To explore the clinical value of MRI three-dimensional visualization （3DV） reconstruction technology in the evaluation of complex anal fistula. Methods The preoperative MRI data of 46 patients with complicated anal fistula confirmed by operation were collected retrospectively and divided into 2D-MRI group and 3DV-MRI group according to the image. The number of internal orifice, fistula and pus cavity were observed respectively, and the images of the two groups were scored according to the scoring standard of Likert scale from the four aspects of clinical concern. Results In the two groups, 64 （81.0%） and 70 （88.6%） internal orifices, 82 （85.4%） and 84 （87.5%） fistulas, 33 （91.7%） and 32 （88.9%） pus cavities were detected respectively. Conclusion Both 2D MRI and 3DV MRI reconstruction images have a high detection rate and accuracy in the preoperative diagnosis of complex anal fistula; Compared with conventional 2D-MRI, 3DV-MRI images can provide more valuable guidance information for clinicians in the diagnosis and treatment of complex anal fistula, increase clinicians' understanding of the extent, scope and adjacent structures of the disease, and have better clinical application value in reducing postoperative recurrence and damage to the anal sphincter.
2023, 44(5): 95-101.
doi: 10.12259/j.issn.2095-610X.S20230511
Abstract:
Objective To investigate the the possible roles of helper T cells 17 （Thl7 cells） and regulatory T cells （Treg cells） of chronic actinic dermatitis. Methods The percentages of Th17 cells and Treg cells in CD4+ T cells in peripheral blood of 25 CAD patients and 20 healthy volunteers were detected by flow cytometry, and the expression of IL-17 and Foxp3 in skin lesions of 25 patients with chronic actinic dermatitis and 10 healthy people were detected by immunohistochemistry. Results Compared with the healthy volunteers, Th17 cells in CAD patients were significantly more than those in volunteers; Treg cells in CAD patients were significantly less than those in volunteers; Th17/Treg in CAD patients was significantly higher than that in volunteers and all the differences were statistically significant （P < 0.05）. The correlation coefficients of Th17 cells and EASI scores showed that there was a positive correlation between them （P < 0.05）; the correlation coefficients of Treg cells and EASI scores showed that there was a negative correlation between them. The expression of IL-17 in lesions of CAD group was higher than that of healthy control group and the difference was statistically significant （P < 0.05）. There was no significant difference in the expression of Foxp3 in the skin compared with the healthy control group （P > 0.05）. Conclusion The imbalance of Th17/Treg in patients with CAD leads to the development of immune response to pro-inflammatory, which may be an important pathogenesis of CAD.
2023, 44(5): 102-106.
doi: 10.12259/j.issn.2095-610X.S20230514
Abstract:
Objective To explore the advantages of esketamine combined with propofol in painless abortion. Methods 50 patients who underwent the painless abortion were selected and randomly divided into the control group and the observation group, with 25 in each group. The control group received Fentanyl combined with propofol intravenous anesthesia while the observation group received Esketamine combined with propofol intravenous anesthesia and the HR, MAP and SpO2 before the induction （T1）, immediately after the operation （T2）, 3 min after the operation （T3） and immediately after the operation （T4） was recorded respectively. The time of consciousness loss and recovery, operation duration and dosage of propofol were recorded. The intraoperative body motor reaction, propofol injection pain, circulation depression, respiratory depression, postoperative adverse reactions and postoperative pain were scored （VAS score）. Results The comparison of the operation time, propofol dose, VAS score and recovery time between the two groups proved to be statistically insignificant （P > 0.05）. At T3, MAP, HR, and SpO2 in the control group were lower than those in the observation group （P < 0.05）; The incidence of injection pain, postoperative dizziness, nausea, and vomiting between the two groups proved to be statistically insignificant （P > 0.05）. The incidence of body movement, respiration, and circulation depression in the observation group was lower than that in the control group （P < 0.05）; The overall incidence of adverse reactions in the observation group was lower than that in the control group （P < 0.05）. Conclusion The application of esketamine combined with propofol in TIVA for painless abortion has been proved to be safe and feasible, and can reduce the incidence of intraoperative body motor reaction, respiratory circulation inhibition and other adverse reactions, and maintain the stability of intraoperative hemodynamics.
2023, 44(5): 107-110.
doi: 10.12259/j.issn.2095-610X.S20230522
Abstract:
Objective To explore the advantages and disadvantages of different approaches of single-incision laparoscopic surgery for the totally extraperitoneal inguinal hernia repair （SILS-TEP）. Methods The data of single-port laparoscopic total extraperitoneal inguinal hernia repair （SILS-TEP） in the same surgical group of the First Affiliated Hospital of Kunming Medical University were retrospectively analyzed. According to the different surgical approaches, 65 patients with the inguinal hernia were divided into two groups: The anterior approach of rectus abdominis posterior sheath group and the posterior approach of rectus abdominis posterior sheath group. The bleeding during the operation, the proportion of peritoneal damage and the recurrence were observed one month after the operation. Results In this study, it was found that there was a significant difference in bleeding volume between the two surgical approaches, with a statistically significant difference （P = 0.001）. There was no statistically significant difference in the proportion of intraoperative peritoneal damage （P = 0.655）. According to the clinical and ultrasound examinations, there were no recurrent cases within 1 month after the surgery. Conclusion The choice of different approaches for SILS-TEP surgery is safe and reliable in experienced surgical teams.The risk of bleeding in the posterior sheath approach of rectus abdominis is slightly higher, but there is no significant difference between the intraoperative peritoneal damage rate and the anterior sheath approach of rectus abdominis. Through reasonable selection of patients, both approaches can achieve satisfactory clinical effects, and it is still suggested that the posterior rectus abdominis sheath anterior approach be more reasonable for beginners.
2023, 44(5): 111-116.
doi: 10.12259/j.issn.2095-610X.S20230507
Abstract:
Objective To investigate the factors affecting the knee joint function recovery time after the platelet-rich plasma and arthroscopy for III degree meniscus injury. Methods From October 1, 2020 to August 20, 2022, 97 patients diagnosed as III degree meniscus injury and treated with the meniscal plasty surgery and the platelet-rich plasma injection in the orthopedic department of Hainan province people’s hospital were recruited in this study. The data including gender, age, and BMI and the injury situation including the lesion limber （left/right）, and the concrete injured meniscus part （red zone and red and white junctional zone） before the operation were collected. After the operation, the patients were required to take the knee exercise for the lesion knee and the lesion knee function was evaluate with Lysholm knee function score. The time taken for the lesion knee function to arrive at 70 scores and above of Lysholm knee function score were recorded. Results The average time for the lesion knee function to arrive at 70 scores and above of Lysholm knee function score was 34.58±6.50 days. The data including gender, age, and BMI and the injury situation including the lesion limber （left/right）, and the concrete injured meniscus part （red zone and red and white junctional zone） were included into the multifactorial COX proportional hazards model, finding age （HR = 0.953, 95%CI = 0.925-0.982, P = 0.002）, BMI （HR = 0.650, 95%CI = 0.549-0.770, P < 0.001）, compared with red zone, red and white junctional zone （HR = 0.463, 95%CI = 0.296-0.726, P = 0.001） might be the important affecting the factors for knee function recovery after the operation, which also was proved by the hierarchical analysis of age and BMI. Conclusion Age and BMI might be the important affecting factors for knee function recovery after the platelet-rich plasma and arthroscopy treatment operation for III degree meniscus injury.
2023, 44(5): 117-124.
doi: 10.12259/j.issn.2095-610X.S20230512
Abstract:
Objective To investigate the predictive effectiveness of different models and the efficacy of baseline T2WI combined with machine learning imaging and to predict the pathological complete remission after the neo-adjuvant chemoradiotherapy （nCRT） in patients with locally advanced rectal cancer （LARC）. Methods A retrospective analysis was conducted on the data of 131 patients with non metastatic advanced rectal cancer from January 2017 to December 2021. All patients underwent the pelvic MRI examination before and after the treatment, received standard nCRT treatment, and then underwent the total mesorectal resection （TME）. AK software （Analysis Kit, GE Healthcare） was used to manually draw the regions of interest （ROI） on the pre-treatment axial T2WI maps, and AK software also extracted the imaging omics features. The imaging omics data were used to build the prediction models by using the support vector machine （SVM）, random forest （RF）, and logistic regression （LR） methods after the the imaging omics features were feature-screened using a two-sample t-test + LASSO regression. The effectiveness of the model prediction was evaluated using the receiver operating characteristic curve （ROC）. Results 26 （19.8%） of the 131 patients had a pathologic complete response （pCR）. The AK software extracted 1308 imaging omics features in total, and after the screening, 12 features were selected for pCR prediction. The SVM model had an AUC, accuracy of 0.8810 and 81.48%, sensitivity and specificity of 90.48% and 50%. The RF model had an AUC, accuracy of 0.7579 and 81.48%, sensitivity and specificity of accuracy 95.24% and 33.33%. The LR model had an AUC, accuracy of 0.9206 and 92.59%, sensitivity and specificity of 95.24% and 83.33%. Conclusion The three machine learning models constructed have the considerable accuracy in predicting complete pathological remission after the concurrent radiotherapy and chemotherapy for locally advanced rectal cancer. Among them, the machine learning model established with the use of logistic regression （LR） method has the higher diagnostic efficiency than other machine learning models, and has the potential to be applied in the clinical practice.
2023, 44(5): 125-131.
doi: 10.12259/j.issn.2095-610X.S20230518
Abstract:
Objective To explore the temperament characteristics of children with attention deficit hyperactivity disorder （ADHD） and to understand its predictive value in children with ADHD. Methods 183 children with ADHD , 45 children with ADHD and treated regularly for half a year and 45 normal controls were selected. They were measured by BSQ\MCTQ. At the same time, Weiss functional defect assessment scale was filled in for ADHD children before and after the treatment. Results In terms of temperament type composition, there was a statistically significant difference among the three groups of ADHD children before and after the treatment, as well as the control group （P < 0.001）. In further analysis, there was a statistically significant difference between the easy type before and after the treatment, and the normal group （P < 0.05）, but there was no statistically significant difference between the treated and normal groups （P > 0.05）; The difference between the difficult type before the treatment and the normal group was statistically significant （P < 0.05）. After six months of the treatment, the proportion of difficult type decreased, while the proportion of easy type increased. After six months of the treatment, the easy type was the main type compared to the normal group, and the difference was not statistically significant （P > 0.05）. In terms of temperament dimensions, there were statistically significant differences between pre treatment and post treatment in seven dimensions: activity level, rhythm, avoidance, adaptability, reaction intensity, emotional essence, and persistence （P < 0.05）. Before the treatment, there were statistically significant differences between the control group and the control group in six dimensions of activity level, rhythm, adaptability, emotional essence, persistence, and attention Dispersity （P < 0.05）.The correlation coefficient between the dimensions of the Temperament scale and WFIRS-P was 0.156～0.428 （P < 0.05）. The multiple linear regression prediction model showed that the adaptability, reaction intensity, persistence, activity level, and emotional essence of the temperament scale had predictive effects on the functions of the WFIRS-P （P < 0.05）. Conclusion The temperament dimension and type composition of children with attention deficit hyperactivity disorder are significantly different from those of normal children of the same age. After half a year of the treatment, the temperament dimension and type composition change significantly, and each dimension of temperament can predict the functional damage of ADHD children.
2023, 44(5): 132-137.
doi: 10.12259/j.issn.2095-610X.S20230515
Abstract:
Objective To study the value of the cervical length （CL） and uterocervical angles （involving anterior uterocervical angles and posterior uterocervical angles） in predicting preterm labor to find new predicting markers via transvaginal ultrasound （TVU） and provide new predicting thoughts. Methods A total of 252 pregnant women selected from 24 to 27+6 weeks of gestation underwent TVU to measure their CL, aUCA and pUCA, and finally were divided into the normal group and the premature group according their delivery time. The difference and value of above markers between the two groups were compared in predicting preterm. Results Among them, 236 gave birth at term, and 16 went into labor prematurely.There was a statistically significant difference in aUCA and pUCA between the two groups （P < 0.05）, while there was no statistically significant difference in CL （ P > 0.05）. By calculating the area under the ROC curve （AUC） of three cervical ultrasound indicators, only the AUC of aUCA was significantly greater than 0.5, with a threshold of 102.22°; The area under the ROC curve for predicting preterm birth using aUCA combined with CL was 0.715, and the threshold for the combined factor was -2.385. Conclusion The predictive value of premature delivery for CL in single live pregnant women aged 24-27+6 weeks is not good. Compared with CL, the measurement of aUCA through TVU is more effective in predicting preterm birth and when measuring aUCA ≥102.22°, the occurrence of premature birth should be highly vigilant. The joint variable combined aUCA and CL is recommended as the predicting marker for pregnant women with single fetus but without any symptom. the predicting value of CL and pUCA need to be studied further.
2023, 44(5): 138-143.
doi: 10.12259/j.issn.2095-610X.S20230524
Abstract:
Objective This study aims to investigate the clinical application of fecal recombinant human adhesin syndecan 2 （SDC2） gene methylation test in the early screening of colorectal tumors. Methods Eighty-seven subjects admitted to the hospital from March 2021 to October 2022 were selected as the study subjects, including 41 cases with normal colorectum, 21 with colorectal adenoma and 25 with colorectal cancer. In this study, the fecal DNA of the subjects was extracted for the fecal SDC2 gene methylation test, and the negative and positive results of the tested specimens were determined according to the Cutoff value set by the kit. The effects of the SDC2 gene methylation test were compared with the results of colonoscopy diagnosis. The analysis of variance on SDC2 gene methylation cycle thresholds （Ct） was performed to assess the expression of SDC2 methylation in colorectal tumors. The subject operating characteristic curve （ROC） was used to calculate the area under the curve （AUC） to evaluate the diagnostic value of SDC2 gene methylation in colorectal tumors and to analyze the sensitivity and specificity of SDC2 gene methylation in colorectal tumor diagnosis. Results Among the 87 enrolled cases, 35 were positive for SDC2 gene methylationand 52 were negative. The positive rate in the stool of colorectal adenoma patients and colorectal cancer patients was 57.14% （12/21） and 84.00% （21/25）, respectively and there were statistically significant differences （P = 0.044）. The results of the analysis under the ROC curve showed that the area of SDC2 gene methylation under the curve （AUC） of the test for the diagnosis of colorectal adenoma and colorectal cancer was 0.715 and 0.918, respectively, with a sensitivity of 57.14% and 88.00% and a specificity of 95.12% and 92.68%, respectively and there were statistically significant differences （P = 0.0107, P < 0.0001）. Conclusion Fecal SDC2 gene methylation has a high specificity for detecting colorectal cancer and its precancerous lesions, and the sensitivity of detection increases as the cancer progresses, so it is of great clinical value in early screening of colorectal cancer.
2023, 44(5): 144-153.
doi: 10.12259/j.issn.2095-610X.S20230502
Abstract:
Objective To observe the effects of asphyxia oxygenation on intersegment plane display rate, intraoperative oxygen metabolism indexes, serum hypoxia inducible factor 1α （HIF-1α） and postoperative complications. Methods 75 patients aged 22 to 65 years with ASA grade I to II were randomly divided into 3 groups, with 25 cases in each group. Group C was given one-lung mechanical ventilation; In T1 group, oxygen was given at the ventilation side of double-lumen endotracheal tube for 3 L/min for asphyxia and oxygenation; In T2 group, oxygen was given for 7L/min through the ventilation side of double-lumen endotracheal tube for asphyxia oxygenation. SpO2, HR, MAP, CO, CI, SV, pH, Hb, PO2/FiO2, PaCO2, A-aO2, Lac, O2Hb, hHb, ScvO2, CvO2, were monitored and recorded before the anesthesia induction （T0）, 15 min after bilateral ventilation （t1）, 15 min after one-lung ventilation （T2）, after double-lung recruitment with pure oxygen （T3）, immediately after the interval plane display （T4） and 15 min after tracheal extubation and DO2, VO2 and O2ER were calculated. The level of HIF-1α was detected by venous blood samples before the surgery, immediately after the surgery and 24 hours after the surgery. The occurrence time of ideal intersegment plane, single lung ventilation time, operation time, postoperative recovery time, extubation time, intraoperative infusion volume, urine volume, blood loss, postoperative hospitalization days, and postoperative complications were recorded. Results （1） The interval plane display time of T1 and T2 groups was shorter than that of C group, and the difference was statistically significant （P < 0.05）. （2） The serum hypoxia induction factor 1α level immediately after the surgery and 24 h after the surgery in 3 groups was higher than that before the surgery, the difference was statistically significant （ P < 0.05）. （3） At T 4, VO2, O2ER, pH, PO2/FiO2, A-aO2 and O2Hb in groups T1 and T2 were lower than those in group C, with the statistical significance （P < 0.05）. ScvO 2, PCO2 and hHb were higher than those in group C, and the difference was statistically significant （P < 0.05）. Conclusion Asphyxiation technique can effectively accelerate the intersegmental plane display rate and ensure the adequate oxygen supply during the operation. It has no obvious influence on the balance of oxygen supply and demand of the body and the intracellular oxygen environment. It has good safety at the level of cells, tissues and organs, and can be applied in the clinical practice.
2023, 44(5): 154-161.
doi: 10.12259/j.issn.2095-610X.S20230523
Abstract:
Objective To analyze the clinical characteristics of nasopharyngeal carcinoma patients in Yunnan on the basis of the clinical data of hospitalized patients, and provide a basis for preventive measures. Methods The clinical data of 2 499 patients with nasopharyngeal carcinoma diagnosed by pathology in Yunnan Cancer Hospital from January 2013 to December 2021 were collected. The incidence trend, region, ethnic distribution, gender and age, family history, first diagnosis symptoms, pathological type, clinical stage, EB virus and EGFR expression were analyzed. Results Among the patients with the nasopharyngeal carcinoma in Yunnan, the ratio of male to female was 2.5∶1, the average age of onset was 49 years old, and the highest age of onset was 41-50 years old. The clinical manifestations were mainly nasal symptoms and neck symptoms. The pathological type was mainly non-keratinizing squamous cell carcinoma, the positive rate of EGFR immunohistochemistry was high （91.57%）, the positive rate of EB virus was nearly 60 %, and the clinical stage was mainly stage III （45.06%） and stage IV （27.45%）. Conclusion The incidence of nasopharyngeal carcinoma in Yunnan is related to EB virus. The positive rate of EGFR is high. It is among the middle-aged and elderly men that the incidence rate of the disease is highest, with the majority being locally advanced patients locally advanced patients and most of them have the cervical lymph node metastasis at the first visit.
2023, 44(5): 162-167.
doi: 10.12259/j.issn.2095-610X.S20230508
Abstract:
Objective To understand the use of primary and menstrual ART regimens for HIV patients receiving antiviral treatment in different periods, and to analyze the dressing change situation and reasons of different drug combinations. Methods Patients initially enrolled in ART from January 2017 to June 2022 were included in the study. The demographic characteristics and the changes of the primary and menstrual ART regimens of the participants in different periods were analyzed and the differences in dressing change rates between the different regimens were compared. Results Most of the subjects were male, The average age was 41.35±14.2 years old and there was an increasing trend （P < 0.001）. TDF/AZT+3TC+EFV was the main ART regimens in newly diagnosed patients, but the utilization rate decreased after the treatment. The TDF+3TC+EFV regimen had the highest dressing change rate （17.3%）. Compared with the initial treatment, the utilization rate of the protocol containing LPV/r increased. Integrase-containing regimens increased significantly in the treated patients in 2021 and early 2022 （P < 0.001）. Drug change rates did not differ at 1, 2, 3, 4, and 5 years of treatment （P = 0.376）. The main reason for dressing change was drug side effects （57.4%）. Conclusion The selection of ART drugs gradually switches from nucleoside and non-nucleosides to integrase inhibitors.The choice of ART drugs is gradually changing from the nucleoside non-nucleoside to integrase inhibitor, which tends to be individualized. Combining the clinical characteristics and complications of patients and choosing the most efficient, low-toxic and simple scheme are more conducive to the long-term effective treatment of patients.
2023, 44(5): 168-174.
doi: 10.12259/j.issn.2095-610X.S20230503
Abstract:
Autophagy is a self-digestive process of cells degrading intracellular substances through lysosomes and is considered to be an important protection mechanism for tumors to maintain survival under stress conditions. A large number of evidence shows that autophagy is a double-edged sword, which can suppress the occurrence of tumors and promote the survival and growth of tumors by different signaling pathways. Therefore, more and more scholars believe that autophagy is expected to become a new strategy for tumor treatment. This paper summarizes the biological behavior of autophagy regulation of bladder cancer invasion, metastasis, and the latest progress in the treatment of bladder cancer and provides new ideas for the development and treatment of autophagy-targeted antitumor drugs.
Autophagy is a self-digestive process of cells degrading intracellular substances through lysosomes and is considered to be an important protection mechanism for tumors to maintain survival under stress conditions. A large number of evidence shows that autophagy is a double-edged sword, which can suppress the occurrence of tumors and promote the survival and growth of tumors by different signaling pathways. Therefore, more and more scholars believe that autophagy is expected to become a new strategy for tumor treatment. This paper summarizes the biological behavior of autophagy regulation of bladder cancer invasion, metastasis, and the latest progress in the treatment of bladder cancer and provides new ideas for the development and treatment of autophagy-targeted antitumor drugs.
2023, 44(5): 175-179.
doi: 10.12259/j.issn.2095-610X.S20230519
Abstract:
Melanoma is a highly lethal skin malignant tumor. Although years of clinical studies have proved that there are various methods for its treatment, such as surgery, chemotherapy, radiotherap, targeted therapy, etc., due to its low sensitivity, strong toxic and side effects, and poor efficacy, in view of the wide application of immunotherapy in the treatment of tumor diseases, the author reviewed the progress of immunotherapy of melanoma in recent years.
Melanoma is a highly lethal skin malignant tumor. Although years of clinical studies have proved that there are various methods for its treatment, such as surgery, chemotherapy, radiotherap, targeted therapy, etc., due to its low sensitivity, strong toxic and side effects, and poor efficacy, in view of the wide application of immunotherapy in the treatment of tumor diseases, the author reviewed the progress of immunotherapy of melanoma in recent years.
2023, 44(5): 180-184.
doi: 10.12259/j.issn.2095-610X.S20230517
Abstract:
Objective To understand the current situation of oral specialty nurses in Yunnan Province at the end of the 13th five year plan period, and to provide a reference basis for formulating the plan of dental specialty in Yunnan Province and building a oral specialty nursing team with the reasonable structure and matching quantity with stomatologists. Methods By January 1st, 2020, the oral care status of all kinds of dental medical institutions at all levels registered with the health administration department in Yunnan Province was surveyed. Results there were 6906 oral nurses in Yunnan Province. 93.20% of them had the primary professional titles, 52.39% had the junior college degrees, and 81.20% were 21-30 years old. The medical care ratio of public dental medical institutions was 1∶0.44, and that of non-public dental medical institutions was 1∶1.19. Conclusion The total number of oral nurses in Yunnan Province is insufficient. The ratio of medical care is low, the age of nurses is younger, the structure of professional titles is low, and the educational level is low. It is suggested that education departments should strengthen the curriculum of oral nursing specialty at the undergraduate stage; Managers of oral medical institutions should pay more attention to the development of oral care and strengthen the training of continuing education of oral care team. General hospitals shall try to increase the allocation of outpatient dental care, improve the ratio of medical care to meet the clinical and sensory control needs.
2023, 44(5): 185-190.
doi: 10.12259/j.issn.2095-610X.S20230510
Abstract:
Objective To explore the effect of nutrition clinical nursing pathway for nasopharyngeal carcinoma patients （NPC） undergoing concurrent radiotherapy and chemotherapy. Methods A total of 162 patients with NPC who received the concurrent radiotherapy and chemotherapy were randomly divided into the control group （n = 81） and experimental group （n = 81）. The control group was received routine nutrition management, while the exprimental group received nutrition clinical nursing pathway intervention. Physiological condition, social/family status, emotional status and functional status of quality of life between the two groups before and after the interven- tion were assessed and compared. Results The scores of functional status and additional attention in the experimental group were significantly higher than those in the control group （P < 0.001）, The score of social/family statusin the experimental group was also significantly higher than that in the control group （P < 0.001）. Conclusion The nutritional clinical nursing pathway can improve the functional status scores and additional attention scores for head and neck tumors, and also played a certain role in improving social/family status scores. To a certain extent, it can effectively improve the quality of life of patients with NPC undergoing radiotherapy and chemotherapy.
