Effect of Gossypol Acetate Acid on Invasion of Human Tongue Squamous Cell Carcinoma Cal-27 Cells in vitro

Si-yu CHEN; Shuai FU; Yong WU

doi:10.12259/j.issn.2095-610X.S20201101

Volume 41 Issue 11

Dec. 2020

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Si-yu CHEN, Shuai FU, Yong WU. Effect of Gossypol Acetate Acid on Invasion of Human Tongue Squamous Cell Carcinoma Cal-27 Cells in vitro[J]. Journal of Kunming Medical University, 2020, 41(11): 12-17. doi: 10.12259/j.issn.2095-610X.S20201101

Citation:

Si-yu CHEN, Shuai FU, Yong WU. Effect of Gossypol Acetate Acid on Invasion of Human Tongue Squamous Cell Carcinoma Cal-27 Cells in vitro[J]. Journal of Kunming Medical University, 2020, 41(11): 12-17. doi: 10.12259/j.issn.2095-610X.S20201101

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Effect of Gossypol Acetate Acid on Invasion of Human Tongue Squamous Cell Carcinoma Cal-27 Cells in vitro

doi: 10.12259/j.issn.2095-610X.S20201101

Dept. of Oral and Maxillofacial Surgery，Affiliated Stomatology Hospital of Kunming Medical University，Kunming Yunnan 650100，China

Received Date: 2020-08-08
Available Online: 2020-12-03
Publish Date: 2020-11-25

Abstract

Abstract

Objective To observe the effect of gossypol acetate acid（GAA）on the invasion of human tongue squamous cell carcinoma Cal-27 cells in vitro. Methods GAA of different concentrations（0 μmol/L, 2.5 μmol/L, 5.0 μmol/L, 10.0 μmol/L）was acted on Cal-27 cells, Transwell experiment was employed to detect the inhibitory effect of GAA on the invasion of Cal-27 cells. The effect of gossypol acetate on MMP-2 and MMP-9 mRNA expression was quantified by qRT-PCR. The effect of gossypol acetate acid on MMP-2 and MMP-9 protein expression was analyzed by Western-Blot experiment. Results Cell Transwell chamber experiment showed that GAA could inhibit the migration of human tongue squamous cell carcinoma Cal-27 cells, and the amount of invasion decreased with the increase in concentration. The difference between the control group and the experimental group was statistically significant（P < 0.05）. qRT-PCR experiment and Western-blot results showed that the expression of MMP-2 and MMP-9 mRNA and proteins was significantly reduced after Cal-27 cells of human tongue squamous cell carcinoma was treated with GAA, and the difference between the control group and the experimental group was statistically significant（P < 0.05）. Conclusions GAA can reduce the invasiveness of tongue squamous cell carcinoma Cal-27 cells. And GAA can reduce the mRNA and protein expression levels of MMP-2 and MMP-9 of human tongue squamous cell carcinoma Cal-27 cells.
- Squamous cell carcinoma of tongue,
- GAA,
- Cell invasion,
- Matrix metalloproteinase-2,
- Matrix metalloproteinase-9

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References(16)

References

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