Mechanism of Biliverdin in Improving Cerebral Ischemia-reperfusion Injury in Rats

Jing LUO; Xian-ling GAO; Jian-lin SHAO; Chao ZHANG; Qi ZHANG

doi:10.12259/j.issn.2095-610X.S20201107

Volume 41 Issue 11

Dec. 2020

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Jing LUO, Xian-ling GAO, Jian-lin SHAO, Chao ZHANG, Qi ZHANG. Mechanism of Biliverdin in Improving Cerebral Ischemia-reperfusion Injury in Rats[J]. Journal of Kunming Medical University, 2020, 41(11): 1-6. doi: 10.12259/j.issn.2095-610X.S20201107

Citation:

Jing LUO, Xian-ling GAO, Jian-lin SHAO, Chao ZHANG, Qi ZHANG. Mechanism of Biliverdin in Improving Cerebral Ischemia-reperfusion Injury in Rats[J]. Journal of Kunming Medical University, 2020, 41(11): 1-6. doi: 10.12259/j.issn.2095-610X.S20201107

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Mechanism of Biliverdin in Improving Cerebral Ischemia-reperfusion Injury in Rats

doi: 10.12259/j.issn.2095-610X.S20201107

1.
Dept. of Anesthesiology, The 1st Affiliated Hospital of Kunming Medical University
2.
Dept. of Anesthesiology，First People’s Hospital of Yunnan Province，Kunming Yunnan 650032
3.
Dept. of Anesthesiology, Pu’er People’s Hospital, Pu’er Yunnan 665000
4.
Dept. of Neurosurgery, The 1st Affiliated Hospital of Kunming Medical University, Kunming Yunnan 650032, China

Received Date: 2020-08-12
Available Online: 2020-12-03
Publish Date: 2020-11-25

Abstract

Abstract

Objective To investigate the protective effect and underlying mechanism of biliverdin on cerebral ischemia-reperfusion injury in rats. Methods The middle cerebral artery occlusion model（MCAO）was established by inserting nylon thread, after biliverdin treatment, the neurological deficit were evaluated by modified neurological severity score, the brain water content were detected by dry and wet weight method, the cerebral infarction were detected by TTC staining, the activation of microglia in ischemic brain tissue were marked by Iba-1/DAPI staining, the expression of TNF-α were detected by immunofluorescence staining, the protein expression of Iba-1/CD68 were detected by Western blot. Results Compared with the IR group, after biliverdin treatment, the neurological deficits were improved（P < 0.05）, Meanwhile, the brain water content was reduced（P < 0.05）, and the cerebral infarction were attenuated（P < 0.05）. What's more, the microglia expression and Iba-1/CD68 protein expression were decreased（P < 0.05）and the inflammatory factor TNF-α expression was also reduced（P < 0.05）. Conclusion Biliverdin may attenuate cerebral ischemia-reperfusion injury by inhibiting microglia activation in rats.
- Biliverdin,
- Microglia,
- Cerebral ischemia reperfusion injury

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References(19)

References

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