Effects of Dexmedetomidine on Inflammatory Response in Rats with Smoke Inhalation Lung Injury

Liu YANG; Qi-cai QU; Rui CHEN; Shu-lan JIAO; Yong-yu SI; Hua ZHOU

doi:10.12259/j.issn.2095-610X.S20201124

Volume 41 Issue 11

Dec. 2020

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Liu YANG, Qi-cai QU, Rui CHEN, Shu-lan JIAO, Yong-yu SI, Hua ZHOU. Effects of Dexmedetomidine on Inflammatory Response in Rats with Smoke Inhalation Lung Injury[J]. Journal of Kunming Medical University, 2020, 41(11): 30-37. doi: 10.12259/j.issn.2095-610X.S20201124

Citation:

Liu YANG, Qi-cai QU, Rui CHEN, Shu-lan JIAO, Yong-yu SI, Hua ZHOU. Effects of Dexmedetomidine on Inflammatory Response in Rats with Smoke Inhalation Lung Injury[J]. Journal of Kunming Medical University, 2020, 41(11): 30-37. doi: 10.12259/j.issn.2095-610X.S20201124

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Effects of Dexmedetomidine on Inflammatory Response in Rats with Smoke Inhalation Lung Injury

doi: 10.12259/j.issn.2095-610X.S20201124

Dept. of Anesthesiology, The 2nd Affiliated Hospital of Kunming Medical University, Kunming Yunnan 650101，China

Received Date: 2020-09-03
Publish Date: 2020-11-25

Abstract

Abstract

Objective To investigate the effects of dexmedetomidine (DEX) on inflammatory response and expression of inflammatory factors in lung tissue of rats with smoke inspiratory lung injury. Methods Seventy-eight healthy and clean adult SD rats were selected from the same batch. Among them, the rats were randomly selected to be injured in the smoke chamber（six rats died）and divided into experimental group (DEX 4.5 ug/kg static injection, n = 32) and control group (same amount of normal saline static injection, n = 32). The remaining 8 rats were used as the blank group without any intervention.According to the different time points of administration, the experimental group and the control group were divided into 4 subgroups, with 8 in each group. The DEX solution was injected through the tail-vein at 4 time points after the smoke injury: half an hour (T1), 12 hours (T2), 24 hours (T3) and 48 hours (T4), respectively.Blood samples were dissected and collected half an hour after administration, and serum and bronchoalveolar lavage fluid levels of tumor necrosis factor (TNF-α) and interleukin-6 (IL-6) were measured by ELISA. Lung tissue was taken to observe the pathological changes of bronchi and alveoli at each time point after injury, and the results were compared with those of the healthy group. Results Compared with the healthy rats in the blank group, all the injured rats were successfully modeled.Compared with the control group, the serum and alveolar lavage fluid of the experimental group showed reduced levels of TNF-αand IL-6 (P < 0.05), especially in the T4stage. TNF-αand IL-6 in the serum of the control group increased continuously with time ( P < 0.05), while TNF-αand IL-6 in the experimental group first increased and then decreased, reaching a peak at 12 hours, and then decreased slowly ( P < 0.05). In alveolar lavage fluid, the inflammatory cytokines in the experimental group increased first and then decreased with time, in which il-6 decreased after reaching the peak at 12 hours, and TNF- radiation decreased after reaching the peak at 24 hours ( P < 0.05). Compared with the healthy group, a large number of inflammatory cells were observed around the alveoli of rats in the experimental group and control group under the light microscope. Moderate and severe inflammatory reactions were dominant in the control group, and mild inflammatory reactions were dominant in the experimental group ( P < 0.05). Conclusion Dexmedetomidine can significantly down-regulate the expression of inflammatory factors in rats with inhalation lung injury, promote the repair of lung tissue injury, and thus play a protective role in lung.
- Dexmedetomidine,
- Inhalation lung injury,
- Burns,
- Inflammatory cytokines,
- Rats

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References(26)

References

[1]	Enkhbaatar P, Pruitt BA, Suman O, et al.Pathophysiology, research challenges, and clinical man-agement of smoke inhalation injury[J]. Lancet, 2016, 388(10): 1437-1446.
[2]	Feng T, Zhou L, Gai S, et al.Acacia catechu (L.f.) Willd and Scutellaria baicalensis Georgi extracts suppress LPS-induced pro-inflammatory responses through NF-кB, MAPK, and PI3K-Akt signaling pathways in alveolar epithelial type Ⅱ cells.[J]Phytother Res.2019, 33(12): 3251-3260. doi: 10.1002/ptr.6499
[3]	贾赤宇, 舒彬. 急性吸入性肺损伤发病机制和临床诊治的相关问题[J]. 中华损伤与修复杂志, 2016, 11(02): 81-83.
[4]	钱何布, 赵宏胜. 右美托咪定的抗炎及器官保护作用研究进展[J]. 医学综述, 2015, 21(15): 2706-2709. doi: 10.3969/j.issn.1006-2084.2015.15.007
[5]	李亮, 李军, 米卫东. 右美托咪定抗炎作用研究进展[J]. 北京医学, 2015, 37(4): 369-371.
[6]	韩霜, 何锟, 张婧, 张秀果, 李建立, 容俊芳. 雾化吸入布地奈德复合沙丁胺醇对单肺通气致兔肺损伤的预防作用及机制探讨[J]. 山东医药, 2020, 60(15): 47-50.
[7]	KISS T, SILVA P L, HUHLE R, et al.Comparison of different degrees of variability in tidal volume to prevent deterioration of respiratory system elastance in experimental acute lung inflammation[J]. Br J Anaesth, 2016, 116(5): 708-715. doi: 10.1093/bja/aew093
[8]	Miller Andrew C, ElaminElaminM, Suffredini Anthony F. Inhaled anticoagulation regimens for the treatment of smoke inhalation-associated acute lung injury: a systematic review.[J]. Pub-med, 2014, 42(2): 413-419.
[9]	Dakhama A, Larsen GL, Gelfand EW. Calcitonin gene-related peptide: role in airway homeostasis.[J]. Curr Opin Pharmacol, 2004, 4(3): 215-220. doi: 10.1016/j.coph.2004.01.006
[10]	Sekizawa S, Joad JP, Bonham AC. Substance P presynaptically depresses the transmission of sensory input to bronchopulmonary neurons in the guinea pig nucleus tractus solitarii.[J]. Physiol, 2003, 552(2): 547-559. doi: 10.1113/jphysiol.2003.051326
[11]	Fontan JJ, Cortright DN, Krause JE, Velloff CR, Karpitskyi VV, Carver Jr TW, et al. Substance P and neurokinin-1 receptor expression by intrinsic airway neurons in the rat.[J]. Physiol Lung Cell Mol Physiol. 2000, 278(2): 344-355.
[12]	Palmieri TL. Inhalation injury: research progress and needs.[J]. J Burn Care Res, 2007, 28(4): 549-554 doi: 10.1097/BCR.0B013E318093DEF0
[13]	You K, Yang HT, Kym D, Yoon J, Haejun Y, Cho YS, et al. Inhalation injury in burn patients: establishing the link between diagnosis and prognosis.[J]. Burns, 2014, 40(8): 1470-1475. doi: 10.1016/j.burns.2014.09.015
[14]	Colohan SM. Predicting prognosis in thermal burns with associated inhalational injury: a systematic review of prognostic factors in adult burn victims.[J]. J Burn Care Res, 2010, 31(4): 529-539 doi: 10.1097/BCR.0b013e3181e4d680
[15]	Wang W, Zhang J, Lv Y, et al.Epidemiological Investigation of Elderly Patients with Severe Burns at a Major Burn Center in Southwest China.[J]. Med Sci Monit, 2020, 26: e918537-1-e918537-12.
[16]	Hamilton TJ, Patterson J, Williams RY, et al.Management of Head and Neck Burns-A 15-Year Review.[J]. J Oral Maxillofac Surg, 2018, 76(2): 375-379. doi: 10.1016/j.joms.2017.09.001
[17]	Joana A, Flávio R.Dexmedetomidine: current role in anesthesia and intensive care.[J]. Rev Bras Anestesiol, 2012, 62(1): 118-133. doi: 10.1016/S0034-7094(12)70110-1
[18]	雷桂玉. 右美托咪定抗炎和脏器保护研究进展[J]. 中国分子心脏病学杂志, 2017, 17(4): 2193-2196.
[19]	Son Ga-Yeon, Shin Dong Min, HongJeongHee. Bacterial PAMPs and Allergens Trigger In-crease in[Ca(2+)]i-induced Cytokine Expression in Human PDL Fibroblasts.[J]. 2015, 19(3): 291-297.
[20]	吴志林, 褚淑娟, 姚尚龙, 伍静. 不同剂量右美托咪定预处理对大鼠心肌缺血再灌注损伤以及炎症反应的影响[J]. 华中科技大学学报(医学版), 2015, 44(4): 445-447.
[21]	Taniguchi Takumi, KuritaAkihide, KobayashiKyoko, YamamotoKen, Inaba Hideo. Dose- and time-related effects of dexmedetomidine on mortality and inflammatory responses to endotoxin-induced shock in rats.[J]. Journal of anesthesia, 2008, 22(3): 221-228. doi: 10.1007/s00540-008-0611-9
[22]	Lili Jiang, Li Li, JinmeiShen, et al. Effect of dexmedetomidine on lung is chemia reperfusion injury. 2014, 9(2): 419-426.
[23]	Geng Y, Li R, He SX, et al.Dexmedetomidine Attenuates Acute Lung Injury Induced by Heatstroke and Improve Outcome.[J]. Shock, 2019, 52(5): 532-539 doi: 10.1097/SHK.0000000000001289
[24]	Liu J, Huang X, Hu S, et al.Dexmedetomidine attenuates lipopolysaccharide induced acute lung injury in rats by inhibition of caveolin-1 downstream signaling.[J]. Biomed Pharmacother, 2019, 118(1): 109-314.
[25]	Liliensiek B, Weigand MA, Bierhaus A, et al. Receptor for advanced glycation end products (RAGE) regulates sepsis but not the adaptive immune response[J]. The Journal of clinical investigation, 2004, 113(2): 1641-1650.
[26]	Zhang Y, Tan X, Xue, L. The alpha2-adrenoreceptor agonist dexmedetomidine protects against lipopolysaccharide-induced apoptosis via inhibition of gap junctions in lung fibroblasts[J]. Biochem Biophys Res Commun, 2018, 495(1): 92-97. doi: 10.1016/j.bbrc.2017.10.162

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