Application of Viral Load Testing in Diagnosing 102 Cases of Indeterminate HIV Antibody

Ting-ting YU; Xiao-fei LI; Hong-ying WANG; Run-wu ZHANG; Cai-mei DING; Li-hua LI; Jing BAI; Dong-ling LI; Dong PU

doi:10.12259/j.issn.2095-610X.S20201126

Volume 41 Issue 11

Dec. 2020

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2020 > 41(11): 130-133

Ting-ting YU, Xiao-fei LI, Hong-ying WANG, Run-wu ZHANG, Cai-mei DING, Li-hua LI, Jing BAI, Dong-ling LI, Dong PU. Application of Viral Load Testing in Diagnosing 102 Cases of Indeterminate HIV Antibody[J]. Journal of Kunming Medical University, 2020, 41(11): 130-133. doi: 10.12259/j.issn.2095-610X.S20201126

Citation:

Ting-ting YU, Xiao-fei LI, Hong-ying WANG, Run-wu ZHANG, Cai-mei DING, Li-hua LI, Jing BAI, Dong-ling LI, Dong PU. Application of Viral Load Testing in Diagnosing 102 Cases of Indeterminate HIV Antibody[J]. Journal of Kunming Medical University, 2020, 41(11): 130-133. doi: 10.12259/j.issn.2095-610X.S20201126

Citation:

Ting-ting YU, Xiao-fei LI, Hong-ying WANG, Run-wu ZHANG, Cai-mei DING, Li-hua LI, Jing BAI, Dong-ling LI, Dong PU. Application of Viral Load Testing in Diagnosing 102 Cases of Indeterminate HIV Antibody[J]. Journal of Kunming Medical University, 2020, 41(11): 130-133. doi: 10.12259/j.issn.2095-610X.S20201126

PDF( 547 KB)

Application of Viral Load Testing in Diagnosing 102 Cases of Indeterminate HIV Antibody

doi: 10.12259/j.issn.2095-610X.S20201126

Clinical Laboratory，The Thid People’s Hospital of Kunming，Kunming Yunnan 650041，China

Received Date: 2020-10-12
Available Online: 2020-12-03
Publish Date: 2020-11-25

Abstract

Abstract

Objective To explore the application value of viral load testing in diagnosing HIV antibody indeterminate cases. Methods The COBAS TaqMan HIV-1 Test v2.0 and CD4 cell count in 102 cases of indeterminate HIV antibody from The Third People's Hospital of Kunming from January 2016 to December 2019 were detected in one week. Patients were followed up to get the blood samples again two to four weeks later for the retest by Western blotting（WB）. The results of the two methods were compared. Results A total 102 cases of blood samples showed unclear WB test result, with 86 cases proved to be HIV positive by follow-up WB test, VL test results of 19 samples more than 20 IU/ml and 2 samples below detection limit 20 IU/ml. 16 cases got confirmed diagnosis of negative HIV antibody by follow-up WB test, and their VL were all TND. Conclusions HIV antibody detection results can be identificated fastly and accurately by high precision detection of viral load. And the time for follow-up will be.shorten.The disease can be accurately judged early. The National Technical Specification for AIDS Testing（2015 Revision）also states that viral load testing as an effective auxiliary detection method for indeterminate samples of HIV antibody. High-precision detection of viral load is of great significance.
- HIV,
- Antibody indeterminate,
- Viral load test,
- Western blot

FullText(HTML)

References(13)

References

[1]	李怡,廖斌,李婕,等. 2010~2015年昆明市嫖客人群艾滋病监测哨点的结果[J].昆明医科大学学报,2019,40(2):139-144. doi: 10.3969/j.issn.1003-4706.2019.02.028
[2]	王立林,赵锦,许晓绚,等. 一例早期治疗HIV窗口期献血者核酸筛查及血清学特性追踪分析[J].中国输血杂志,2018,31(12):1345-1348.
[3]	中华医学会感染病学分会艾滋病学组,Diseases SoI,Association C M. 艾滋病诊疗指南第三版(2015版)[J].中华临床感染病杂志,2015,8(5):385-401. doi: 10.3760/cma.j.issn.1674-2397.2015.05.001
[4]	Schwartlander B,Stover J,Hallett T,et al. Towards an improved investment approach for an effective response to HIV/AIDS[J]. Lancet,2011,377(9782):1-11.
[5]	中国疾病预防控制中心. 全国艾滋病检测技术规范（2015修订版）[J]. 中国病毒病杂志, 2016, （6）: 401-427.
[6]	彭瑾瑜,贺健梅,江洋,等. 病毒载量试验对“HIV-1抗体不确定”病例诊断的效果评价[J].中国艾滋病性病,2018,24(9):866-869.
[7]	冯霞,娄金丽,刘翠娥,等. HIV抗体不确定带型与临床转归的研究[J].北京医学,2019,41(6):448-450.
[8]	黄丹梅. 71例HIV抗体不确定检测结果及随访分析[J].中国医师杂志,2019,21(2):272-274. doi: 10.3760/cma.j.issn.1008-1372.2019.02.031
[9]	尤佳女,陈兵,许珂. HIV抗体确证实验不确定样本的筛查及随访[J].国际流行病学传染病学杂志,2019,46(1):34-38. doi: 10.3760/cma.j.issn.1673-4149.2019.01.008
[10]	马仲慧,陈兵,常浩,等. 非HIV感染者蛋白印迹试验不确定及假阳性结果的原因[J].中国艾滋病性病,2017,23(6):571-574.
[11]	黑发欣,张启云,孙伟东,等. 病毒载量检测鉴别诊断HIV早期感染[J].中华微生物学和免疫学杂志,2008,28(6):557-559. doi: 10.3321/j.issn:0254-5101.2008.06.021
[12]	Zhao J,Bai J,Shen K L,et al. Self-reported prevalence of childhood allergic diseases in three cities of China:A multicenter study[J]. BMC Public Health,2010,10:551-558. doi: 10.1186/1471-2458-10-551
[13]	BrownE,Chi B H,Read J S,et a1. Determining an optimal testing strategy for infants at risk for mother-to-child transmission of HIV-1 during the late postnatal period[J]. AIDS,2008,22(17):2341-2346. doi: 10.1097/QAD.0b013e328317cc15

Relative Articles(20)

Relative Articles

[1]	Yihan LIU, Jun LIU, Yongmei JIN, Bo TIAN, Jinsong BAI. Risk Assessment and Analysis of Risk Factors for Cardiovascular Disease in HIV Antiviral Therapy Patients. Journal of Kunming Medical University, 2024, 45(10): 140-146. doi: 10.12259/j.issn.2095-610X.S20241022
[2]	Xuelin XIANG, Hongrui ZHAO, Zhongjie WANG, Yu XIA, Min MA, Huadan WANG, Liping. HE. Social Support Status and Influencing Factors of HIV-infected Patients on Antiviral Therapy. Journal of Kunming Medical University, 2024, 45(9): 156-162. doi: 10.12259/j.issn.2095-610X.S20240924
[3]	Bo TIAN, Yongmei JIN, Haiwen LI, Chongxi LI, Wei ZHANG, Wei GUAN, Haiyun CHEN, Qi XUE, Huirong YANG, Jun LIU. Analysis of the Current Status and Influencing Factors of Metabolic Syndrome in Patients Undergoing Antiretroviral Therapy for AIDS. Journal of Kunming Medical University, 2024, 45(11): 1-6.
[4]	Bo TIAN, Jun LIU, Chongxi LI, Wei ZHANG, Meiqin FANG, Haiyun CHEN, Jingping BAI, Xin ZHOU, Lei CHENG, Yongmei JIN. Effect Observation and Influencing Factors Analysis of Rapid Initiation of Antiretroviral Therapy. Journal of Kunming Medical University, 2024, 45(9): 163-167. doi: 10.12259/j.issn.2095-610X.S20240925
[5]	Bo TIAN, Chongxi LI, Yongmei JIN, Wei GUAN, Haiyun CHEN, Jun LIU. Use and Replacement of Treatment Regimens for AIDS Patients on Antiretroviral Therapy. Journal of Kunming Medical University, 2023, 44(5): 162-167. doi: 10.12259/j.issn.2095-610X.S20230508
[6]	Bo TIAN, Jun LIU, Haiwen LI, Wei SONG, Haiyun CHEN, Jianjun SUN. Pathogen Distribution and Antimicrobial Susceptibility of Bacterial Bloodstream Infection in AIDS Patients. Journal of Kunming Medical University, 2022, 43(6): 140-146. doi: 10.12259/j.issn.2095-610X.S20220616
[7]	Bo TIAN, Yinzhong SHEN, Jinsong BAI, Jun LIU, Haiyun CHEN, Jianjun SUN. Clinical Characteristics of Talaromyces Marneffei Disease and Disseminated Nontuberculosis Mycobacterium Disease in AIDS Patients. Journal of Kunming Medical University, 2022, 43(7): 140-144. doi: 10.12259/j.issn.2095-610X.S20220718
[8]	Le ZHANG, Yuanqiang HE, Jiawei XIA, Shu LIU, Bin BAI, Xing LIU. Effect of Early Interventional Therapy with Bronchoscopic Alveolar Lavage on AIDS Patients Complicated with Severe Pneumonia. Journal of Kunming Medical University, 2022, 43(11): 151-155. doi: 10.12259/j.issn.2095-610X.S20221120
[9]	Diandian ZENG, Bo TIAN, Yongmei JIN, Chongxi LI, Fen TENG, Jun LIU. A Cross-sectional Survey on Quality of Life of AIDS Patients with Different Antiretroviral Regimens in Kunming Area. Journal of Kunming Medical University, 2022, 43(12): 147-152. doi: 10.12259/j.issn.2095-610X.S20221227
[10]	Shan HUANG, Song-qin LV, Hong-fen DUAN, Jin-li SHI, Guang-min LI, Gang HUANG, Jia-neng LI, Xiao-fei LI. Cohort Study on the Influence of Opportunistic Infectious Pathogens on the Distribution of Peripheral Blood T Lymphocyte Subsets in Newly Acquired AIDS Patients During Antiretroviral Therapy. Journal of Kunming Medical University, 2021, 42(11): 159-165. doi: 10.12259/j.issn.2095-610X.S20211128
[11]	Zhang Le , Peng Jing , Xia Jia Wei , Yue Yun Xuan . . Journal of Kunming Medical University, 2018, 39(12): 115-119.
[12]	Wang Wei Bo , Jin Yong Mei , Yang Jun Jie , Qian Li Fang , Fang Mei Qin , Bai Jing Song . Satisfaction Analysis on the Application of Mobile Nursing Station in HIV/AIDS Department. Journal of Kunming Medical University, 2018, 39(07): 146-148.
[13]	Zhang Lu Ping , Han Yue Hong , Chen Gui Rong , Zhang Wen Jie , Zhao Xia , Yu Xiao Mei . A Study on the Preventive Drugs for Exposure to AIDS. Journal of Kunming Medical University, 2018, 39(02): 124-127.
[14]	Ma Xue Ting , Li Ya . The Analysis of T Lymphocyte Subset Among Newly-discovered HIV Infectors and Patients. Journal of Kunming Medical University, 2017, 38(11): 112-117.
[15]	Feng Jun , Wang Shang Wen , Zhang Zheng , Li Yu Ming . Investigation on HIV/AIDS Health Education Demand of Undergraduates from Different Type Universities in Multi-ethnic Frontier Regions. Journal of Kunming Medical University, 2017, 38(08): 122-125.
[16]	Pan Song Feng . . Journal of Kunming Medical University,
[17]	Zhao Guang Yu . . Journal of Kunming Medical University,
[18]	Fu Zhong Yang . . Journal of Kunming Medical University,
[19]	Su Yun Peng . . Journal of Kunming Medical University,
[20]	. . Journal of Kunming Medical University,