Genetic Characteristic Analysis of Coxsackievirus A16 in Wenshan Prefecture of Yunnan Province in 2019

Li-fen ZHANG; Xue-hua ZHOU; Min XU; Li-li JIANG; Jian-ping CUN; Xiao-fang ZHOU; Xiao-qing FU; Bing-jun TIAN

doi:10.12259/j.issn.2095-610X.S20201128

Volume 41 Issue 11

Dec. 2020

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Article Navigation > Journal of Kunming Medical University > 2020 > 41(11): 18-24

Li-fen ZHANG, Xue-hua ZHOU, Min XU, Li-li JIANG, Jian-ping CUN, Xiao-fang ZHOU, Xiao-qing FU, Bing-jun TIAN. Genetic Characteristic Analysis of Coxsackievirus A16 in Wenshan Prefecture of Yunnan Province in 2019[J]. Journal of Kunming Medical University, 2020, 41(11): 18-24. doi: 10.12259/j.issn.2095-610X.S20201128

Citation:

Li-fen ZHANG, Xue-hua ZHOU, Min XU, Li-li JIANG, Jian-ping CUN, Xiao-fang ZHOU, Xiao-qing FU, Bing-jun TIAN. Genetic Characteristic Analysis of Coxsackievirus A16 in Wenshan Prefecture of Yunnan Province in 2019[J]. Journal of Kunming Medical University, 2020, 41(11): 18-24. doi: 10.12259/j.issn.2095-610X.S20201128

Citation:

Li-fen ZHANG, Xue-hua ZHOU, Min XU, Li-li JIANG, Jian-ping CUN, Xiao-fang ZHOU, Xiao-qing FU, Bing-jun TIAN. Genetic Characteristic Analysis of Coxsackievirus A16 in Wenshan Prefecture of Yunnan Province in 2019[J]. Journal of Kunming Medical University, 2020, 41(11): 18-24. doi: 10.12259/j.issn.2095-610X.S20201128

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Genetic Characteristic Analysis of Coxsackievirus A16 in Wenshan Prefecture of Yunnan Province in 2019

doi: 10.12259/j.issn.2095-610X.S20201128

1.
Yunnan Center for Disease Control & Prevention，Kunming Yunnan 650022
2.
Wenshan Prefecture Center for Disease Control & Prevention，Wenshan Yunnan 663099
3.
Yunnan Institute of Science & Technology，Kunming Yunnan 650000，China

Received Date: 2020-08-11
Publish Date: 2020-11-25

Abstract

Abstract

Objective To isolate the enterovirus（EV）from 595 stool samples collected from hand, foot and mouth disease（HFMD）patients from Wenshan prefecture of Yunnan province in 2019 and to analyze the epidemiology of EVs and the genetic characteristics of coxsackievirus A16（CV-A16）. Methods Virus isolated from 595 stool samples was carried out and the VP1 gene of 74 positive samples was amplified and identified by sequencing and the genetic characteristics and molecular epidemiology of CV-A16 were analyzed. Results Seventy-four strains of EVs were isolated from 595 samples with an isolation rate of 12.44（74/595）, of which, 72 strains were enterovirus species A（EV-A）, accounting for 97.30%（72/74）, 2 strains were EV-B, accounting for 2.70%（2/74）, EV-C and EV-D viruses were not isolated. Among EV-A viruses, CV-A6 was most frequently isolated, accounting for 61.11%（44/72）, CV-A16 was the second, accounting for 19.44%（14/72）, 9 strains were CV-A10, accounting for 12.50%（9/72）. The genetic characteristics analysis showed that all 14 strains of CV-A16 belonged to subgenotype B1, they were further divided into 2 subgenotypes（B1a and B1b）, among which, 8 strains were B1a subgenotype, 6 strains were B1b subgenotype. Conclusions The main etiology of HFMD in Wenshan prefecture in 2019 was CV-A6, CV-A16 was the second. Two subgenotypes（B1a and B1b）of CV-A16 co-circulated in Wenshan prefecture in 2019.
- Hand, foot and mouth disease,
- Etiology analysis,
- Coxsackievirus A16,
- VP1 gene,
- Genetic characteristics analysis

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References(25)

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