Significance of miRNA-21 Expression in Rabbits with High-Dose Fractional Radiation Heart Injury

Shu-hui YU; Jing-yan GAO; Biao ZHAO; Yu-tao ZHAO; Ya LI

doi:10.12259/j.issn.2095-610X.S20201204

Volume 41 Issue 12

Dec. 2019

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Shu-hui YU, Jing-yan GAO, Biao ZHAO, Yu-tao ZHAO, Ya LI. Significance of miRNA-21 Expression in Rabbits with High-Dose Fractional Radiation Heart Injury[J]. Journal of Kunming Medical University, 2020, 41(12): 1-5. doi: 10.12259/j.issn.2095-610X.S20201204

Citation:

Shu-hui YU, Jing-yan GAO, Biao ZHAO, Yu-tao ZHAO, Ya LI. Significance of miRNA-21 Expression in Rabbits with High-Dose Fractional Radiation Heart Injury[J]. Journal of Kunming Medical University, 2020, 41(12): 1-5. doi: 10.12259/j.issn.2095-610X.S20201204

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Significance of miRNA-21 Expression in Rabbits with High-Dose Fractional Radiation Heart Injury

doi: 10.12259/j.issn.2095-610X.S20201204

Radiotherapy Center，The 3rd Affiliated Hospital of Kunming Medical University / Yunnan Cancer Hospital，Kunming Yunnan 650118，China

Received Date: 2020-04-10
Publish Date: 2019-12-26

Abstract

Abstract

Objective To establish a good, reliable, and stable radiation-induced heart disease （RIHD）model, and to determine the miRNA-21 expression trend in plasma. Methods 27 adult male healthy New Zealand rabbits were randomly divided into control group （3） and irradiation group （24）. After anesthetized with 3% pentobarbital sodium （a dose of 0.8-1.0 ml/kg）, the rabbits were scanned by CT to determine target area by sketch the front wall of the left ventricle for the irradiation. Linear accelerator （Elekta Precise）, 6 MV high-energy X-ray, 5 Gy/F, 2 F/W irradiation, every 3 days until the total dose is 30 Gy. Rabbits in the control group were given sham irradiation after anesthesia. At 6, 24, 72 hours and 1, 2, 4, 8, and 24 weeks after irradiation, plasma was collected from the marginal ear vein to detect the expression of miRNA-21 by RT-PCR, and the heart tissue was collected to examine heart damage by HE staining. Results The inflammatory cells infiltrated between myocardial fibers after irradiation were mainly mononuclear cells. The edema of myocardial cells was obvious and the arrangement was disordered. The nucleus of myocardial cells was slightly contracted and the staining darkened. A few heterotypic nuclei were present, which was consistent with cardiac injury model lesions. miR-21 detection by RT-PCR was elevated and statistically significant at each time point compared with the control group （P < 0.05）. With the irradiation time course at 6 h, 24 h, 72 h, 1 w, 2 w, and 4 w, respectively, miR-21 expression continued to increase gradually and reached the peak expression at 4 w; miR-21 expression decreased in 8 w and 24 w indicates expression was reducing with irradiation time （ P < 0.05）. Conclusion Multiple doses and large doses of irradiation can build a good, reliable, and stable RIHD ladder model, which can better simulate the evolution of the occurrence and development of RIHD. Detection of miR-21 expression in plasma can initially predict the progression of RIHD fibrosis.
- Radiation-induced,
- Heart disease,
- miR-21

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References(27)

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