Characteristics of Intestinal Microflora in Patients with Coronary Heart Disease

Hai-long DAI; Nan WANG; Xiao-qing CHEN; Zhen-yi HE; Xiao-lan FENG; Xue-feng GUANG

doi:10.12259/j.issn.2095-610X.S20201213

Volume 41 Issue 12

Dec. 2019

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Article Navigation > Journal of Kunming Medical University > 2020 > 41(12): 36-41

Hai-long DAI, Nan WANG, Xiao-qing CHEN, Zhen-yi HE, Xiao-lan FENG, Xue-feng GUANG. Characteristics of Intestinal Microflora in Patients with Coronary Heart Disease[J]. Journal of Kunming Medical University, 2020, 41(12): 36-41. doi: 10.12259/j.issn.2095-610X.S20201213

Citation:

Hai-long DAI, Nan WANG, Xiao-qing CHEN, Zhen-yi HE, Xiao-lan FENG, Xue-feng GUANG. Characteristics of Intestinal Microflora in Patients with Coronary Heart Disease[J]. Journal of Kunming Medical University, 2020, 41(12): 36-41. doi: 10.12259/j.issn.2095-610X.S20201213

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Characteristics of Intestinal Microflora in Patients with Coronary Heart Disease

doi: 10.12259/j.issn.2095-610X.S20201213

1.
Dept. of Cardiology，Yan’an Hospital，Kunming Medical University/Key Laboratory of Cardiovascular Disease of Yunnan Province
2.
Dept. of Emergency，Yan’an Hospital，Kunming Medical University，Kunming Yunnan 650051
3.
School of Medicine，Kunming University of Science and Technology，Kunming Yunnan 650500，China

Received Date: 2020-03-05
Publish Date: 2019-12-26

Abstract

Abstract

Objective To analyze the composition and diversity of intestinal microflora in patients with coronary heart disease in Yunnan, and to explore the differences of intestinal microflora among different types of coronary heart disease and those without coronary artery disease. Methods 67 patients with acute myocardial infarction（group A）, 91 patients with unstable angina（group B）, 47 patients with stable angina（group C）, 64 patients without abnormal coronary arteries（group D）and 37 patients with coronary atherosclerosis（group E）were enrolled. Faeces sample DNA was extracted. Specific primers were designed according to 16SrRNA v3-v4 sequence of bacteria, PCR amplification was carried out, and sequenced with Illumina hiseq PE250. The sequencing results were analyzed by species composition statistics, Alpha diversity, intergroup difference analysis, and finally get the species information of the faeces sample. Results There were differences in the composition of intestinal flora among groups（P < 0.05）. Compared with group D, there were more Clostridium in group A, less Bacteroides, more Proteus and actinomycetes in group B, less Bacteroides, more Proteus and Clostridium in group C, and more Proteus in group E（ P < 0.05）. The alpha diversity of AMI patients was higher than that of other groups（ P < 0.05）. Conclusion The composition, diversity and abundance of intestinal flora in coronary atherosclerotic group, stable angina group, unstable angina group, acute myocardial infarction group and control group were different in Yunnan, and they had intestinal flora disorder at various degrees.
- Coronary artery disease,
- Atherosclerosis,
- Intestinal microorganism,
- 16srRNA

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References(24)

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