Efficacy of PCR-reverse Dot Hybridization in Drug Resistance Gene Detection and Drug Sensitivity of BD960 Tuberculosis in Drug Resistance Tuberculosis Detection

Xiao-qin LI; Min ZHOU; Lin WANG; Hai-yan ZENG; Peng-ju LUO; Li-jia LIN

doi:10.12259/j.issn.2095-610X.S20201238

Volume 41 Issue 12

Dec. 2019

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Article Navigation > Journal of Kunming Medical University > 2020 > 41(12): 138-141

Xiao-qin LI, Min ZHOU, Lin WANG, Hai-yan ZENG, Peng-ju LUO, Li-jia LIN. Efficacy of PCR-reverse Dot Hybridization in Drug Resistance Gene Detection and Drug Sensitivity of BD960 Tuberculosis in Drug Resistance Tuberculosis Detection[J]. Journal of Kunming Medical University, 2020, 41(12): 138-141. doi: 10.12259/j.issn.2095-610X.S20201238

Citation:

Xiao-qin LI, Min ZHOU, Lin WANG, Hai-yan ZENG, Peng-ju LUO, Li-jia LIN. Efficacy of PCR-reverse Dot Hybridization in Drug Resistance Gene Detection and Drug Sensitivity of BD960 Tuberculosis in Drug Resistance Tuberculosis Detection[J]. Journal of Kunming Medical University, 2020, 41(12): 138-141. doi: 10.12259/j.issn.2095-610X.S20201238

Citation:

Xiao-qin LI, Min ZHOU, Lin WANG, Hai-yan ZENG, Peng-ju LUO, Li-jia LIN. Efficacy of PCR-reverse Dot Hybridization in Drug Resistance Gene Detection and Drug Sensitivity of BD960 Tuberculosis in Drug Resistance Tuberculosis Detection[J]. Journal of Kunming Medical University, 2020, 41(12): 138-141. doi: 10.12259/j.issn.2095-610X.S20201238

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Efficacy of PCR-reverse Dot Hybridization in Drug Resistance Gene Detection and Drug Sensitivity of BD960 Tuberculosis in Drug Resistance Tuberculosis Detection

doi: 10.12259/j.issn.2095-610X.S20201238

1.
Dept. of Laboratory
2.
Dept. of Tuberculosis，The 3rd People’ s Hospital of Kunming，Kunming Yunnan 650301，China

Received Date: 2020-04-14
Publish Date: 2019-12-26

Abstract

Abstract

Objective To analyze the difference between PCR-reverse dot hybridization drug resistance gene detection and BD960 Mycobacterium tuberculosis drug sensitivity test in identifying drug resistance of Mycobacterium tuberculosis, and to discuss its clinical efficacy. Methods The drug sensitivity of isoniazid (H), rifampicin (R), ethambutanol (E) and streptomycin (S) in sputum and lavage samples of 356 hospitalized TB patients were collected in The 3rd People's Hospital of Kunming were detected simultaneously by two testing methods. The results were compared and analyzed by correction χ² test. Results Single drug resistance, multi-drug resistance and MDR were detected by the two testing methods. On the 99% confidence interval, the adjusted chi-square values are all P < 0.01. Conclusion The two methods have differences in the detection of three kinds of resistance. The multidrug resistance PCR-reverse dot blot hybridization method of Mycobacterium tuberculosis drug sensitivity test is better than BD960 detection. The drug sensitivity of single drug resistance and multi-drug resistance BD960 is better than PCR-reverse dot hybridization.
- Mycobacterium tuberculosis,
- Drug resistance,
- Gene detection,
- BD960 sensitivity

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References(14)

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