OCT Evaluation on the Establishment of A Mouse Model of Chronic Ocular Hypertension Induced by Magnetic Beads Injection

Xiu-qiang YAN; Xiao-fan ZHANG; Yu-ling REN; Juan-juan LI; Li-wei ZHANG

doi:10.12259/j.issn.2095-610X.S20201245

Volume 42 Issue 1

Jan. 2021

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Xiu-qiang YAN, Xiao-fan ZHANG, Yu-ling REN, Juan-juan LI, Li-wei ZHANG. OCT Evaluation on the Establishment of A Mouse Model of Chronic Ocular Hypertension Induced by Magnetic Beads Injection[J]. Journal of Kunming Medical University, 2021, 42(1): 23-28. doi: 10.12259/j.issn.2095-610X.S20201245

Citation:

Xiu-qiang YAN, Xiao-fan ZHANG, Yu-ling REN, Juan-juan LI, Li-wei ZHANG. OCT Evaluation on the Establishment of A Mouse Model of Chronic Ocular Hypertension Induced by Magnetic Beads Injection[J]. Journal of Kunming Medical University, 2021, 42(1): 23-28. doi: 10.12259/j.issn.2095-610X.S20201245

Citation:

Xiu-qiang YAN, Xiao-fan ZHANG, Yu-ling REN, Juan-juan LI, Li-wei ZHANG. OCT Evaluation on the Establishment of A Mouse Model of Chronic Ocular Hypertension Induced by Magnetic Beads Injection[J]. Journal of Kunming Medical University, 2021, 42(1): 23-28. doi: 10.12259/j.issn.2095-610X.S20201245

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OCT Evaluation on the Establishment of A Mouse Model of Chronic Ocular Hypertension Induced by Magnetic Beads Injection

doi: 10.12259/j.issn.2095-610X.S20201245

Xiu-qiang YAN¹,
Xiao-fan ZHANG^{2, 3, 4, 5},
Yu-ling REN^{2, 3, 4, 5},
Juan-juan LI^{2, 3, 4, 5},
Li-wei ZHANG^{2, 3, 4, 5
,
,}

1.
Dept. of Ophthalmology，People’s Hospital of Diqing Tibetan Autonomous Prefecture，Diqing Yunnan，674400
2.
Dept. of Ophthalmology，The Second People’s Hosptial of Yunnan Province，Kunming Yunnan，650031
3.
Dept.of Ophthalmology，The Affiliated Hospital of Yunnan University，Kunming Yunnan，650031
4.
Yunnan Eye Institute，Yunnan Clinical Medical Research Center of Eye Disease，Kunming Yunnan，650031
5.
Yunnan Clinical Research Center of Eye Disease，Kunming Yunnan 650031，China

Received Date: 2020-07-29
Available Online: 2021-01-26
Publish Date: 2021-01-26

Abstract

Abstract

Objective To explore the feasibility and characteristics of magnetic microbead anterior chamber injection inducing ocular hypertension model, and to study the role of OCT in model evaluation. Methods C57BL/6 mice were divided into the normal group, control group and experimental group. The normal group was not treated. The intraocular pressure fluctuations were detected before the surgery and on the first day after the surgery; After 3 weeks of modeling, fluorescent gold retrograde labeling was performed, and after 4 weeks of modeling, OCT was used to detect the thickness of para-optical nerve fibers in two groups of mice, and the number of RGCs in each group was counted by parallel retinal plating. Results After the injection of magnetic fluorescent microbeads into the anterior chamber of the mice, the microbeads were able to be evenly distributed at the angle of the anterior chamber to block the angle. In the experimental group, the intraocular pressure of the mice began to increase 1 day after the injection, and the average intraocular pressure was 19.37 ± 4.38 mmHg. The intraocular pressure decreased slightly after three weeks. In the 4th week, OCT was used to detect the thickness of RNFL. The thickness of RNFL in the experimental group was 27.67 ± 6.15 μm, which was significantly thinner than that in the control group. The difference between the two groups was statistically significant（p = 0.0082）. After detecting OCT, RGCs were counted in wholemount retina and the RGCs in the experimental group were 203.83 ± 26.35, which was significantly reduced compared with the control group, and the difference was statistically significant（p = 0.0094）. Conclusion The injection of magnetic microbeads in the anterior chamber of mice can induce a sustained and stable increase in intraocular pressure and cause a significant decrease in the number of RGCs. RGC damage can be found by non-invasive, rapid, and repeated detection of the thickness of the nerve fiber layer by OCT.
- Magnetic microbeads,
- High intraocular pressure,
- OCT,
- Glaucoma,
- Animal model

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References(20)

References

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