The Regulation of Exosome Derived from Human Bone Marrow Mesenchymal Stem Cells on the Polarization of Glioma-associated Macrophages

Xiao CAI; Xue-tao YI; Jing-qing YAO; Xin-yu DAI; Zhong-quan TANG; Ting OU; Xiao-min ZHAO; Yun-tao LI

doi:10.12259/j.issn.2095-610X.S20210101

Volume 42 Issue 1

Jan. 2021

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Article Navigation > Journal of Kunming Medical University > 2021 > 42(1): 38-45

Xiao CAI, Xue-tao YI, Jing-qing YAO, Xin-yu DAI, Zhong-quan TANG, Ting OU, Xiao-min ZHAO, Yun-tao LI. The Regulation of Exosome Derived from Human Bone Marrow Mesenchymal Stem Cells on the Polarization of Glioma-associated Macrophages[J]. Journal of Kunming Medical University, 2021, 42(1): 38-45. doi: 10.12259/j.issn.2095-610X.S20210101

Citation:

Xiao CAI, Xue-tao YI, Jing-qing YAO, Xin-yu DAI, Zhong-quan TANG, Ting OU, Xiao-min ZHAO, Yun-tao LI. The Regulation of Exosome Derived from Human Bone Marrow Mesenchymal Stem Cells on the Polarization of Glioma-associated Macrophages[J]. Journal of Kunming Medical University, 2021, 42(1): 38-45. doi: 10.12259/j.issn.2095-610X.S20210101

Citation:

Xiao CAI, Xue-tao YI, Jing-qing YAO, Xin-yu DAI, Zhong-quan TANG, Ting OU, Xiao-min ZHAO, Yun-tao LI. The Regulation of Exosome Derived from Human Bone Marrow Mesenchymal Stem Cells on the Polarization of Glioma-associated Macrophages[J]. Journal of Kunming Medical University, 2021, 42(1): 38-45. doi: 10.12259/j.issn.2095-610X.S20210101

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The Regulation of Exosome Derived from Human Bone Marrow Mesenchymal Stem Cells on the Polarization of Glioma-associated Macrophages

doi: 10.12259/j.issn.2095-610X.S20210101

1.
Dept. of Neurology，The Second Affiliated Hospital of Nanjing Medical University，Wenling Zhejiang 317500
2.
Qingdao Institute of Food and Drug Control，Qingdao Shandong 266000，China

Received Date: 2020-06-15
Publish Date: 2021-01-25

Abstract

Abstract

Objective To investigate the effect of exosomes divided from human bone marrow mesenchymal stem cells on the polarization of tumor-associated macrophages, and to preliminarily discuss their influence on glioma cell lines. Methods Exosomes divided from human bone marrow mesenchymal stem cells were extracted and identified by scanning electron microscopy and western blot. The glioma cell line SHG449 was divided into three groups: SHG449 group: SHG449 cells without special treatment; SHG449+M0 group: SHG449 cells co-cultured with M0 macrophages; SHG449+M0+exosome group: SHG449 cells co-cultured with M0 macrophages, and then exosomes labeled with PKH-67 were added to the M0 cells. The mRNA expression of M1 biomarkers（iNOS and CD68）and M2 markers（Arginase and CD206）were detected by qPCR, and the content of IL-1β, TNF-α, CCL22 and TGF-β in the supernatant of SHG449 cells were detected by ELISA. The proliferation and apoptosis of SHG449 cell line were detected by CCk8 and flow cytometry. Results （1）Exosomes secreted by human bone marrow mesenchymal stem cells were successfully extracted and entered into M0-type macrophages.（2）Macrophage morphology was transformed after exosome was intaken by SHG449-induced polarized macrophages, and the expression of M2 macrophages markers Arginase, CD206, CCL22 and TGF-β were decreased（Arginase: P < 0.01, CD206: P < 0.05, CCL22: P < 0.05, TGF-β: P < 0.01）, while the expression of M1 macrophages markers iNOS, CD68, IL-1β and TNF-α increased（iNOS: P < 0.01, CD68: P < 0.01, L-1β: P < 0.000 1, TNF-α: P < 0.001）.（3）Macrophage polarization induced by exosomes resulted in decreased proliferation（ P < 0.05） and increased apoptotic（ P < 0.000 1） of glioma cells. Conclusion Exosomes derived from human bone marrow stromal cells can inhibit the polarization of tumor-associated macrophages to the M2 phenotype and induce their polarization to the M1 phenotype, and regulate the tumor immune microenvironment, thereby suppressing the development of glioma.
- Malignant glioma,
- Macrophage polarization,
- Exosomes,
- Human bone marrow mesenchymal stem cells.

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