The Effect of Peptide-prolinyl Isomerase（Pin1）on Lipid Metabolism in Cervical Cancer Cells

Li-jun HAO; Li-xiu XU; Jin-qiu LI; Jun-qi MA; Hasimu AXIANGU

doi:10.12259/j.issn.2095-610X.S20210111

Volume 42 Issue 1

Jan. 2021

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Li-jun HAO, Li-xiu XU, Jin-qiu LI, Jun-qi MA, Hasimu AXIANGU. The Effect of Peptide-prolinyl Isomerase（Pin1）on Lipid Metabolism in Cervical Cancer Cells[J]. Journal of Kunming Medical University, 2021, 42(1): 12-16. doi: 10.12259/j.issn.2095-610X.S20210111

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Li-jun HAO, Li-xiu XU, Jin-qiu LI, Jun-qi MA, Hasimu AXIANGU. The Effect of Peptide-prolinyl Isomerase（Pin1）on Lipid Metabolism in Cervical Cancer Cells[J]. Journal of Kunming Medical University, 2021, 42(1): 12-16. doi: 10.12259/j.issn.2095-610X.S20210111

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The Effect of Peptide-prolinyl Isomerase（Pin1）on Lipid Metabolism in Cervical Cancer Cells

doi: 10.12259/j.issn.2095-610X.S20210111

1.
Dept. of Laboratory，People’s Hospital of Xinjiang Uygur Autonomous Region，Urumqi Xinjiang 830001
2.
Basic Medicine College of Xinjiang Medical University，Urumqi Xinjiang 830011
3.
Dept. of Gynecology，The 1st Affiliated Hospital of Xinjiang Medical University，Urumqi Xinjiang 830011，China

Received Date: 2020-11-26
Publish Date: 2021-01-25

Abstract

Abstract

Objective To investigate the effect of peptide-based prolinyl isomerase（Pin1）protein on lipid metabolism in cervical cancer cells. Methods The expression of Pinl and ACC1 protein in chronic cervical cancer and cervical cancer tissues was detected by immunohistochemistry. Western blotting and RT-PCR were used to detect the background expression of Pin1 gene in SiHa, C33a and H8 cells of cervical cancer, and RNA interference was used to detect the expression of Pin1 in C33a cells of cervical cancer. The influence of Pin1 protein expression on ACC1 protein expression in cervical cancer cells was detected by Western blotting. Lipid-soluble fluorescence staining（bodipy493/503）was used to observe the changes in the content of neutral fat in cervical cancer cells before and after transfection with low expression of lentivirus Pin1. Results Compared with chronic cervicitis, the expression of Pin1 and ACC1 in cervical cancer tissues was significantly up-regulated（P < 0.05）, and the expression of Pin1 and ACC1 in cervical cancer tissues was positively correlated（r = 4.45, P < 0.05）. Compared with C33a cells, the expression level of Pin1 protein in SiHa cells was relatively low, so C33a cells were selected for transfection with lentivirus with low Pin1 expression. After the transfection with lentivirus with low Pin1 expression in C33a cells, the protein expression levels of Pin1 and ACC1 were significantly decreased（ P < 0.05）. Compared with normal control group and negative control group, the content of intracellular neutral fatty acids transfected with lentivirus with low Pin1 expression was decreased. Conclusion The expression of Pin1 protein is involved in lipid metabolism in cervical cancer cells, and the specific regulatory mechanism remains to be studied.
- Cervical cancer,
- Peptide-prolinyl isomerase,
- Fatty acid synthetase

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References(12)

References

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