Effect of Laser Artificial Shrinkage on Pregnancy Outcome of Single Blastocysts Transfer with Different Developmental Days and Stages in the Frozen-thawed Cycles

Xiang LI; Yu-di LUO; Ju-guang ZHANG; Juan HE; Bo-wen LUO; Jun-jie ZHONG; Song-yuan TANG; Xiang-qian MENG

doi:10.12259/j.issn.2095-610X.S20210114

Volume 42 Issue 1

Jan. 2021

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Xiang LI, Yu-di LUO, Ju-guang ZHANG, Juan HE, Bo-wen LUO, Jun-jie ZHONG, Song-yuan TANG, Xiang-qian MENG. Effect of Laser Artificial Shrinkage on Pregnancy Outcome of Single Blastocysts Transfer with Different Developmental Days and Stages in the Frozen-thawed Cycles[J]. Journal of Kunming Medical University, 2021, 42(1): 152-156. doi: 10.12259/j.issn.2095-610X.S20210114

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Effect of Laser Artificial Shrinkage on Pregnancy Outcome of Single Blastocysts Transfer with Different Developmental Days and Stages in the Frozen-thawed Cycles

doi: 10.12259/j.issn.2095-610X.S20210114

1.
Reroductive Medicine Center，Maternal and Child Health Hospital of Yulin，Yulin Guangxi 573000
2.
School of Public Health，Kunming Medical University，Kunming Yunnan 650500
3.
Reproductive Medicine Center，Maternal and Child Health Hospital of Chengdu Jinjiang，Chengdu Sichuan 610016，China

Received Date: 2020-09-14
Available Online: 2021-01-26
Publish Date: 2021-01-26

Abstract

Abstract

Objective To investigate the effect of laser artificial shrinkage on the pregnancy outcome of single blastocysts transfer with different developmental days and stages in in the frozen-thawed cycles Methods The clinical data of 336 cases of frozen thawed single blastocyst transplantation cycles in reproductive center of Yulin maternal and Children Health Hospital in Guangxi were analyzed retrospectively. According to whether laser shrinkage was performed, the patients were divided into laser shrinkage group（227 cases）and non-shrinkage group（106 cases）. According to the development time of blastocyst, the experimental group was divided into five groups: shrinkage D5 group, shrinkage D6 group, non shrinkage D5 group and non shrinkage D6 group. Then according to the score of cell mass and trophoblast layer in blastocyst, the experimental group was divided into three groups: shrinkage D5 group, shrinkage D6 group, non shrinkage D5 group and non shrinkage D6 group. The clinical outcomes of cryothaw transfer of single blastocysts with different developmental time and different grades were compared among the three groups. Results Compared with the single blastocyst and with the same development time, the abortion rate of D5 group with laser shrinkage was higher than that of D5 group without shrinkage, and the difference was statistically significant（P < 0.05）. There were no statistically significant differences in the other outcome indicators between the wrinkled group and the non-wrinkled group（P > 0.05）. Compared with the single blastocysts of the same grade, the pregnancy rate of the wrink-led embryo group was significantly higher than that of the other groups（P > 0.05）. Conclusion Laser shrinkage can improve the clinical pregnancy rate of high quality single blastocyst transplantation but it can also increase the risk of D5 single blastocyst abortion rate.
- In vitro fertilization-embryo transfer,
- Single blastocyst transfer,
- Artificial shrinkage,
- Vitrification

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