Effect of Laser Artificial Shrinkage on Pregnancy Outcome of Single Blastocysts Transfer with Different Developmental Days and Stages in the Frozen-thawed Cycles

Xiang LI; Yu-di LUO; Ju-guang ZHANG; Juan HE; Bo-wen LUO; Jun-jie ZHONG; Song-yuan TANG; Xiang-qian MENG

doi:10.12259/j.issn.2095-610X.S20210114

Volume 42 Issue 1

Jan. 2021

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Article Navigation > Journal of Kunming Medical University > 2021 > 42(1): 152-156

Xiang LI, Yu-di LUO, Ju-guang ZHANG, Juan HE, Bo-wen LUO, Jun-jie ZHONG, Song-yuan TANG, Xiang-qian MENG. Effect of Laser Artificial Shrinkage on Pregnancy Outcome of Single Blastocysts Transfer with Different Developmental Days and Stages in the Frozen-thawed Cycles[J]. Journal of Kunming Medical University, 2021, 42(1): 152-156. doi: 10.12259/j.issn.2095-610X.S20210114

Citation:

Xiang LI, Yu-di LUO, Ju-guang ZHANG, Juan HE, Bo-wen LUO, Jun-jie ZHONG, Song-yuan TANG, Xiang-qian MENG. Effect of Laser Artificial Shrinkage on Pregnancy Outcome of Single Blastocysts Transfer with Different Developmental Days and Stages in the Frozen-thawed Cycles[J]. Journal of Kunming Medical University, 2021, 42(1): 152-156. doi: 10.12259/j.issn.2095-610X.S20210114

Citation:

Xiang LI, Yu-di LUO, Ju-guang ZHANG, Juan HE, Bo-wen LUO, Jun-jie ZHONG, Song-yuan TANG, Xiang-qian MENG. Effect of Laser Artificial Shrinkage on Pregnancy Outcome of Single Blastocysts Transfer with Different Developmental Days and Stages in the Frozen-thawed Cycles[J]. Journal of Kunming Medical University, 2021, 42(1): 152-156. doi: 10.12259/j.issn.2095-610X.S20210114

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Effect of Laser Artificial Shrinkage on Pregnancy Outcome of Single Blastocysts Transfer with Different Developmental Days and Stages in the Frozen-thawed Cycles

doi: 10.12259/j.issn.2095-610X.S20210114

1.
Reroductive Medicine Center，Maternal and Child Health Hospital of Yulin，Yulin Guangxi 573000
2.
School of Public Health，Kunming Medical University，Kunming Yunnan 650500
3.
Reproductive Medicine Center，Maternal and Child Health Hospital of Chengdu Jinjiang，Chengdu Sichuan 610016，China

Received Date: 2020-09-14
Available Online: 2021-01-26
Publish Date: 2021-01-26

Abstract

Abstract

Objective To investigate the effect of laser artificial shrinkage on the pregnancy outcome of single blastocysts transfer with different developmental days and stages in in the frozen-thawed cycles Methods The clinical data of 336 cases of frozen thawed single blastocyst transplantation cycles in reproductive center of Yulin maternal and Children Health Hospital in Guangxi were analyzed retrospectively. According to whether laser shrinkage was performed, the patients were divided into laser shrinkage group（227 cases）and non-shrinkage group（106 cases）. According to the development time of blastocyst, the experimental group was divided into five groups: shrinkage D5 group, shrinkage D6 group, non shrinkage D5 group and non shrinkage D6 group. Then according to the score of cell mass and trophoblast layer in blastocyst, the experimental group was divided into three groups: shrinkage D5 group, shrinkage D6 group, non shrinkage D5 group and non shrinkage D6 group. The clinical outcomes of cryothaw transfer of single blastocysts with different developmental time and different grades were compared among the three groups. Results Compared with the single blastocyst and with the same development time, the abortion rate of D5 group with laser shrinkage was higher than that of D5 group without shrinkage, and the difference was statistically significant（P < 0.05）. There were no statistically significant differences in the other outcome indicators between the wrinkled group and the non-wrinkled group（P > 0.05）. Compared with the single blastocysts of the same grade, the pregnancy rate of the wrink-led embryo group was significantly higher than that of the other groups（P > 0.05）. Conclusion Laser shrinkage can improve the clinical pregnancy rate of high quality single blastocyst transplantation but it can also increase the risk of D5 single blastocyst abortion rate.
- In vitro fertilization-embryo transfer,
- Single blastocyst transfer,
- Artificial shrinkage,
- Vitrification

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References(15)

References

[1]	Vanderzwalmen P,Bertin G,Debauche C,et al. Births after vitrification at morula and blastocyst stages:effect of artificial reduction of the blastocoelic cavity before vitrification[J]. Hum Reprod,2002,17(3):744-751. doi: 10.1093/humrep/17.3.744
[2]	Gardner D K,Schoolcraft W B. Culture and transfer of human blastocysts[J]. Curr Opin Obstet Gynecol,1999,11(3):307-311. doi: 10.1097/00001703-199906000-00013
[3]	Joo J K,Jeong J E,Kim C W,et al. Comparison of mechanical artificial shrinkage methods in mouse blastocyst vitrification[J]. Clin Exp Obstet Gynecol,2016,43(1):93-97. doi: 10.1016/j.ogc.2015.10.005
[4]	Darwish E,Magdi Y. Artificial shrinkage of blastocoel using a laser pulse prior to vitrification improves clinical outcome[J]. J Assist Reprod Genet,2016,33(4):467-471. doi: 10.1007/s10815-016-0662-z
[5]	Son W Y,Yoon S H,Yoon H J,et al. Pregnancy outcome following transfer of human blastocysts vitrified on electron microscopy grids after induced collapse of the blastocoele[J]. Hum Reprod,2003,18(1):137-139. doi: 10.1093/humrep/deg029
[6]	李翔,曾莠蔓,罗宇迪,等. 体外受精-胚胎移植中移植不同培育时间、单双胚胎的不孕症患者受孕及妊娠结局观察[J].山东医药,2019,59(27):67-70. doi: 10.3969/j.issn.1002-266X.2019.27.019
[7]	Tubbing A,Shaw-Jackson C,Ameye L,et al. Increased live births after day 5 versus day 6 transfers of vitrified-warmed blastocysts[J]. J Assist Reprod Genet,2018,35(3):417-424. doi: 10.1007/s10815-017-1097-x
[8]	任文娟,师娟子,李明昭,等. 激光人工皱缩对囊胚玻璃化冷冻后妊娠结局的影响[J].西安交通大学学报(医学版),2018,39(2):281-285.
[9]	Li L,Zhang X,Zhao L,et al. Comparison of DNA apoptosis in mouse and human blastocysts after vitrification and slow freezing[J]. Mol Reprod Dev,2012,79(3):229-236. doi: 10.1002/mrd.22018
[10]	冯贵雪,张波,舒金辉,等. 人工皱缩囊胚腔对囊胚玻璃化冷冻后妊娠结局的影响[J].中华妇产科杂志,2010,5(11):838-842. doi: 10.3760/cma.j.issn.0529-567x.2010.11.010
[11]	Huang C C,Lee T H,Chen S U,et al. Successful pregnancy following blastocyst cryopreservation using super-cooling ultra-rapid vitrification[J]. Hum Reprod,2005,20(1):122-128. doi: 10.1093/humrep/deh540
[12]	Thompson S M,Onwubalili N,Brown K,et al. Blastocyst expansion score and trophectoderm morphology strongly predict successful clinical pregnancy and live birth following elective single embryo blastocyst transfer(eSET):a national study[J]. J Assist Reprod Genet,2013,30(12):1577-1581. doi: 10.1007/s10815-013-0100-4
[13]	甄璟然,王雪,孙正怡,等. 复苏单囊胚移植周期中囊胚形态学评分对临床妊娠的影响[J].生殖医学杂志,2014,23(1):7-10. doi: 10.3969/j.issn.1004-3845.2014.01.002
[14]	Honnma H,Baba T,Sasaki M,et al. Trophectoderm morphology significantly affects the rates of ongoing pregnancy and miscarriage in frozen-thawed single-blastocyst transfer cycle in vitro fertilization[J]. Fertil Steril,2012,98(2):361-367. doi: 10.1016/j.fertnstert.2012.05.014
[15]	Kovacic B,Taborin M,Vlaisavljevic V. Artificial blastocoel collapse of human blastocysts before vitrification and its effect on re-expansion after warming-A prospective observational study using time-lapse microscopy[J]. Reprod Biomed Online,2018,36(2):121-129. doi: 10.1016/j.rbmo.2017.10.111

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