Effect of Tumor Antigen Loaded DC-CIK on Microenvironment of Hepatocellular Carcinoma

Da-guang TIAN; Heng-hai YU; Xiao-ping WEI

doi:10.12259/j.issn.2095-610X.S20210127

Volume 42 Issue 1

Jan. 2021

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Da-guang TIAN, Heng-hai YU, Xiao-ping WEI. Effect of Tumor Antigen Loaded DC-CIK on Microenvironment of Hepatocellular Carcinoma[J]. Journal of Kunming Medical University, 2021, 42(1): 46-50. doi: 10.12259/j.issn.2095-610X.S20210127

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Da-guang TIAN, Heng-hai YU, Xiao-ping WEI. Effect of Tumor Antigen Loaded DC-CIK on Microenvironment of Hepatocellular Carcinoma[J]. Journal of Kunming Medical University, 2021, 42(1): 46-50. doi: 10.12259/j.issn.2095-610X.S20210127

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Effect of Tumor Antigen Loaded DC-CIK on Microenvironment of Hepatocellular Carcinoma

doi: 10.12259/j.issn.2095-610X.S20210127

The First Department of HBP Surgery，The 2nd Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650101，China

Received Date: 2020-10-11
Available Online: 2021-01-26
Publish Date: 2021-01-26

Abstract

Abstract

Objective To study the effect of tumor antigen loaded DC-CIK on the microenvironment of hepatocellular carcinoma. Methods The antigen of HCC coming from resected HCC and peripheral blood mononuclear cells were isolated from healthy donors. The cells were divided into 4 groups as DC, Ag-DC, CIK and Ag-DC-CIK according to the loading of HCC antigen or coculture of DC-CIK or not. Cell phenotype of 4 groups were evaluated by FCM. Furthermore, levels of IL-6, IL-10 and IFN-γ; expression of VEGF and MMP-2、MMP-3、MMP-9mRNA were observed. Results The contents of CD80, CD86 in group Ag-DC and CD3⁺CD56⁺, CD8⁺CD56⁺ double positive cells in group Ag-DC-CIK elevated significantly（P < 0.05）. The level of IL-6 and IFN-γdecreased significantly in group Ag-DC-CIK（P < 0.05）. Expression of VEGF and MMP-2, MMP-3, MMP-9mRNA also reduced significantly in group Ag-DC-CIK（P < 0.05）. Conclusion Coculture of DC and CIK on the basis of loading HCC antigen shows the stronger activity and reduces the expression of relating factors about inflammation, angiopoiesis and fibrosis in HePG2 microenvironment.
- Hepatocellular carcinoma,
- Microenvironment,
- Dendritic cell,
- Cytokine-induced killer

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