Volume 42 Issue 1
Jan.  2021
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Yang Hong Yu , Zhao Shi Hao , Zhao Rui , Lv Yu , He Xiao Guang , Peng Yun Zhu , Yin Zhu Ping , Zhang Jing Jing , Lin Yan . The Incidence of Pharyngeal Fistula after Total Laryngectomy and the Dynamic Analysis of Antimicrobial Use[J]. Journal of Kunming Medical University, 2018, 39(04): 70-72.
Citation: Da-guang TIAN, Heng-hai YU, Xiao-ping WEI. Effect of Tumor Antigen Loaded DC-CIK on Microenvironment of Hepatocellular Carcinoma[J]. Journal of Kunming Medical University, 2021, 42(1): 46-50. doi: 10.12259/j.issn.2095-610X.S20210127
shu

Effect of Tumor Antigen Loaded DC-CIK on Microenvironment of Hepatocellular Carcinoma

doi: 10.12259/j.issn.2095-610X.S20210127

  • The First Department of HBP Surgery，The 2nd Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650101，China

  • Received Date: 2020-10-11
    Available Online: 2021-01-26
  • Publish Date: 2021-01-26
    Abstract
  •   Objective  To study the effect of tumor antigen loaded DC-CIK on the microenvironment of hepatocellular carcinoma.  Methods  The antigen of HCC coming from resected HCC and peripheral blood mononuclear cells were isolated from healthy donors. The cells were divided into 4 groups as DC, Ag-DC, CIK and Ag-DC-CIK according to the loading of HCC antigen or coculture of DC-CIK or not. Cell phenotype of 4 groups were evaluated by FCM. Furthermore, levels of IL-6, IL-10 and IFN-γ; expression of VEGF and MMP-2、MMP-3、MMP-9mRNA were observed.  Results  The contents of CD80, CD86 in group Ag-DC and CD3+CD56+, CD8+CD56+ double positive cells in group Ag-DC-CIK elevated significantly（P < 0.05）. The level of IL-6 and IFN-γdecreased significantly in group Ag-DC-CIK（P < 0.05）. Expression of VEGF and MMP-2, MMP-3, MMP-9mRNA also reduced significantly in group Ag-DC-CIK（P < 0.05）.  Conclusion  Coculture of DC and CIK on the basis of loading HCC antigen shows the stronger activity and reduces the expression of relating factors about inflammation, angiopoiesis and fibrosis in HePG2 microenvironment.
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    • References(18)
  • [1]
    Mak L Y. Editorial:Can hepatitis B core-related antigen be the new biomarker for hepatocellular carcinoma in nucleoside analogue-treated chronic hepatitis B?[J]. Aliment PharmacolTher,2019,49(7):954-955. doi: 10.1111/apt.15143
    [2]
    Zhang J,Li H,Gao D,et al. A prognosis and impact factor analysis ofDC-CIK cell therapy for patients with hepatocellular carcinoma undergoing postoperative TACE[J]. Cancer Biol Ther,2018,19(6):475-483. doi: 10.1080/15384047.2018.1433501
    [3]
    Zhang B,Zhang B,Zhang Z,et al. 42,573 cases of hepatectomy in China:a multicenter retrospective investigation[J]. Sci China Life Sci,2018,61(6):660-670. doi: 10.1007/s11427-017-9259-9
    [4]
    Schmidt N,Thimme R. Role of immunity in pathogenesis and treatment of hepatocellular carcinoma[J]. Dig Dis,2016,34(4):429-437. doi: 10.1159/000444558
    [5]
    Yoon J S,Song B G,Lee J H,et al. Adjuvant cytokine-induced killer cell immunotherapy for hepatocellular carcinoma:A propensity score-matched analysis of real-world data[J]. BMC Cancer,2019,19(1):523. doi: 10.1186/s12885-019-5740-z
    [6]
    Park R,Eshrat F,Al-Jumayli M,et al. Immuno-Oncotherapeutic approaches in advanced hepatocellular carcinoma[J]. Vaccines (Basel),2020,8(3):447. doi: 10.3390/vaccines8030447
    [7]
    Wang S,Wang X,Zhou X,et al. DC-CIK as a widely applicable cancer immunotherapy[J]. Expert Opin Biol Ther,2020,20(6):601-607. doi: 10.1080/14712598.2020.1728250
    [8]
    Zhou Z,Qin H,Weng L,Ni Y. Clinical efficacy of DC-CIK combined with sorafenib in the treatment of advanced hepatocellular carcinoma[J]. J BUON,2019,24(2):615-621.
    [9]
    Kurebayashi Y,Ojima H,Tsujikawa H,et al. Landscape of immune microenvironment in hepatocellular carcinoma and its additional impact on histological and molecular classification[J]. Hepatology,2018,68(3):1025-1041. doi: 10.1002/hep.29904
    [10]
    Di Bella L M,Alampi R,Biundo F,et al. Copper chelation andinterleukin-6 proinflammatory cytokine effects on expression of different proteins involved in iron metabolism in HepG2 cell line[J]. BMC Biochem,2017,18(1):1. doi: 10.1186/s12858-017-0076-2
    [11]
    Li Changfei,Deng Mengmeng,Hu Jun,et al. Chronic inflammation contributes to the development of hepatocellular carcinoma by decreasing miR-122 levels[J]. Oncotarget,2016,7(13):17021-17034. doi: 10.18632/oncotarget.7740
    [12]
    Dai W,Wang Y,Yang T,et al. Down regulation of exosomal CLEC3B in hepatocellular carcinoma promotes metastasis and angiogenesis via AMPK and VEGF signals[J]. Cell Commun Signal,2019,17(1):113. doi: 10.1186/s12964-019-0423-6
    [13]
    Yao Y,Wang T,Liu Y,et al. Co-delivery of sorafenib and VEGF-siRNA via pH-sensitive liposomes for the synergistic treatment of hepatocellular carcinoma[J]. Artif Cells Nanomed Biotechnol,2019,47(1):1374-1383. doi: 10.1080/21691401.2019.1596943
    [14]
    唐诗聪,陈东,郭瑢,等. miRNA-21调控HER-2阳性乳腺癌的血管生成[J].昆明医科大学学报,2020,41(8):39-45.
    [15]
    雷喜锋,侯峰强,杨少华,等. miR-373在人肝细胞癌中的表达及其作用[J].昆明医科大学学报,2018,39(12):49-53. doi: 10.3969/j.issn.1003-4706.2018.12.010
    [16]
    Sumer S,Aktug Demir N,Kölgelier S,et al. The clinical significance of serum apoptotic cytokeratin 18 neoepitope M30 (CK-18 M30) and matrix metalloproteinase 2 (MMP-2) levels in chronic hepatitis B patients with cirrhosis[J]. Hepat Mon,2013,13(6):e10106.
    [17]
    Boukhedouni N,Martins C,Darrigade A S,et al. Type-1 cytokines regulate MMP-9 production and E-cadherin disruption to promote melanocyte loss in vitiligo[J]. JCI Insight,2020,5(11):e133772.
    [18]
    刘坤,朱颖,董蕙,等. 老年晚期肺癌高凝状态患者外周血VEGF、MMP-9的表达[J].昆明医科大学学报,2017,38(1):46-50. doi: 10.3969/j.issn.1003-4706.2017.01.010
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    [20] . Inducing Culture in Vitro and Identification of IFN- γProducing Killer Dendritic Cells. Journal of Kunming Medical University,
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