Dexmedetomidine Inhibits Neuronal Apoptosis after Traumatic Brain Injury in Rats

Yan CAO; Hong-yan JIANG; Yan-xue WANG; Dou-wei WU; Chuan-yun QIAN; Hai-ying WU; Ping LI

doi:10.12259/j.issn.2095-610X.S20210202

Volume 42 Issue 2

Mar. 2021

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Yan CAO, Hong-yan JIANG, Yan-xue WANG, Dou-wei WU, Chuan-yun QIAN, Hai-ying WU, Ping LI. Dexmedetomidine Inhibits Neuronal Apoptosis after Traumatic Brain Injury in Rats[J]. Journal of Kunming Medical University, 2021, 42(2): 1-5. doi: 10.12259/j.issn.2095-610X.S20210202

Citation:

Yan CAO, Hong-yan JIANG, Yan-xue WANG, Dou-wei WU, Chuan-yun QIAN, Hai-ying WU, Ping LI. Dexmedetomidine Inhibits Neuronal Apoptosis after Traumatic Brain Injury in Rats[J]. Journal of Kunming Medical University, 2021, 42(2): 1-5. doi: 10.12259/j.issn.2095-610X.S20210202

Citation:

Yan CAO, Hong-yan JIANG, Yan-xue WANG, Dou-wei WU, Chuan-yun QIAN, Hai-ying WU, Ping LI. Dexmedetomidine Inhibits Neuronal Apoptosis after Traumatic Brain Injury in Rats[J]. Journal of Kunming Medical University, 2021, 42(2): 1-5. doi: 10.12259/j.issn.2095-610X.S20210202

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Dexmedetomidine Inhibits Neuronal Apoptosis after Traumatic Brain Injury in Rats

doi: 10.12259/j.issn.2095-610X.S20210202

1.
Dept. of Human Anatomy and Histology & Embryolog，Kunming Medical University，Kunming Yunnan 650500
2.
Dept. of Emergency，Zhengzhou Central Hospital Affiliated to Zhengzhou University，Henan Zhengzhou 450007
3.
Dept. of Emergency，The First Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

Received Date: 2020-12-09
Publish Date: 2021-03-05

Abstract

Abstract

Objective To discuss whether the neuroprotective effect of dexmedetomidine （DEX） against traumatic brain injury （TBI） is related to the inhibition of neuronal apoptosis and whether it is involved in SIRT1 signaling pathway. Methods SD rats were randomly divided into 4 groups: Sham group, TBI + NS group, TBI + DEX group and TBI + DEX + EX527 group. The water content of brain tissue after traumatic brain injury was evaluated by dry/wet weight method; the neurological function was evaluated by neurological severity score; the expression of Bcl-2 and Bax proteins in rat cortex were detected by Western blot. Results DEX decreased the expression of apoptosis-promoting regulatory protein Bax （P < 0.05）, increased the expression of apoptosis-inhibiting regulatory protein Bcl-2 （P < 0.05）, alleviated brain edema （P < 0.05） and improvedthe neurological function after traumatic brain injury （P < 0.05）. The changes of the above indexes were reversed by SIRT1 inhibitor EX527. Conclusions DEX can reduce the secondary damage after traumatic brain injury by inhibiting the apoptosis of nerve cells. There may be a certain correlation between the neuroprotective effect and SIRT1 signaling pathway.
- Dexmedetomidine,
- Traumatic brain injury,
- Neuronal apoptosis

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References(20)

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