Role of Phospholipase C epsilon 1 in Neuropathic Pain of Rats with Type 1 Diabetes

Xin GUO; Wei LAI; Yan-hua LI; Yun-ru CHEN; Ao-ran YE; Shun-song TANG; Jing LUO

doi:10.12259/j.issn.2095-610X.S20210206

Volume 42 Issue 2

Mar. 2021

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Xin GUO, Wei LAI, Yan-hua LI, Yun-ru CHEN, Ao-ran YE, Shun-song TANG, Jing LUO. Role of Phospholipase C epsilon 1 in Neuropathic Pain of Rats with Type 1 Diabetes[J]. Journal of Kunming Medical University, 2021, 42(2): 23-28. doi: 10.12259/j.issn.2095-610X.S20210206

Citation:

Xin GUO, Wei LAI, Yan-hua LI, Yun-ru CHEN, Ao-ran YE, Shun-song TANG, Jing LUO. Role of Phospholipase C epsilon 1 in Neuropathic Pain of Rats with Type 1 Diabetes[J]. Journal of Kunming Medical University, 2021, 42(2): 23-28. doi: 10.12259/j.issn.2095-610X.S20210206

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Role of Phospholipase C epsilon 1 in Neuropathic Pain of Rats with Type 1 Diabetes

doi: 10.12259/j.issn.2095-610X.S20210206

1.
Dept. of Anesthesiology，The First People’s Hospital of Yunnan Province，Kunming Yunnan 650032
2.
Dept. of Pain Management，The 1st People’s Hospital of Yunnan Province，Kunming Yunnan 650032，China

Received Date: 2020-11-14
Publish Date: 2021-03-05

Abstract

Abstract

Objective To elucidate the pathogenic role of phospholipase C epsilon 1 （PLCE1） in neuropathic pain of rats with type 1 diabetes by observing the effect of intrathecal injection of exoenzyme C3 （a RhoA inhibitor） on the activity of PLCE1 and inflammatory response in the spinal cord. Methods Twenty-four 8-week-old healthy male Sprague-Dawley （SD） rats were randomly allocated to healthy rats plus vehicle group （Group C）, healthy rats plus exoenzyme C3 group （Group E）, type 1 diabetic neuropathic pain rats induced by streptozotocin （STZ） plus vehicle group （Group DNP） and type 1 diabetic neuropathic pain rats plus exoenzyme C3 group （Group EG）. The diabetic neuropathic pain model of STZ -induced type 1 diabetes in rats was established by a single intravenous injection of STZ （65 mg/kg）. In Group E and Group EG, 10 µL exoenzyme C3 （1 pg/µL ） was injected intrathecally once a day for 7 consecutive days, whereas vehicle was injected intrathecally in Group C and Group DNP. Accu-Chek Compact Plus glucose meter was used to measure fasting blood glucose concentration （mmol/L） in the tail vein once weekly. Western blot was used to detect the expressions of total RhoA and PLCE1 in the spinal cord in rats, while the RhoA activity detection kit was used to measure the expression of RhoA-GTP protein. The expression of PLCE1 protein and the ratio of RhoA-GTP/ total RhoA were used to estimate the activities of PLCE1 and RhoA, respectively. Enzyme-linked immunosorbent assay （ELISA） was used to measure the contents of TNF-α and IL-6 in spinal cord to evaluate proinfammatory cytokines- induced the inflammation. The severity of DNP was evaluated by thermal withdrawal latency （TWL） and mechanical withdrawal threshold （MWT）. Results No significant difference was detected between group C and group E. Rats with DNP had a decreased pain threshold （P < 0.05）, up-regulated activities of RhoA and PLCE1 （P < 0.05） and increased production of TNF-α and IL-6 in spinal cord （P < 0.05） as compared to the group C and group E. Intrathecal injection of exoenzyme C3 rather than vehicle decreased the activity of RhoA in spinal cord （P < 0.05） of type 1 DNP rats, accompanied by a down-regulation of PLCE1 activity and proinflammatory mediators （P < 0.05） and a relief of DNP （P < 0.05）. Conclusion PLCE1 plays an important role in type 1 diabetic neuropathic pain by promoting spinal cord inflammation, and its activity is regulated by RhoA.
- Diabetic neuropathic pain,
- Phospholipase C epsilon 1,
- RhoA,
- Exoenzyme C3,
- Inflammation

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References(18)

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