Application of Ultrasound-Targeted Microbubble Destruction with IL-6 Monoclonal Antibody in Rabbit Myocardial Ischemia-Reperfusion Injury

Yuan YANG; Hong-min LIANG; Gui-min ZHU; Han-ning YANG; Wei-han CAO; Yong-ping LU

doi:10.12259/j.issn.2095-610X.S20210222

Volume 42 Issue 2

Mar. 2021

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Yuan YANG, Hong-min LIANG, Gui-min ZHU, Han-ning YANG, Wei-han CAO, Yong-ping LU. Application of Ultrasound-Targeted Microbubble Destruction with IL-6 Monoclonal Antibody in Rabbit Myocardial Ischemia-Reperfusion Injury[J]. Journal of Kunming Medical University, 2021, 42(2): 6-12. doi: 10.12259/j.issn.2095-610X.S20210222

Citation:

Yuan YANG, Hong-min LIANG, Gui-min ZHU, Han-ning YANG, Wei-han CAO, Yong-ping LU. Application of Ultrasound-Targeted Microbubble Destruction with IL-6 Monoclonal Antibody in Rabbit Myocardial Ischemia-Reperfusion Injury[J]. Journal of Kunming Medical University, 2021, 42(2): 6-12. doi: 10.12259/j.issn.2095-610X.S20210222

Citation:

Yuan YANG, Hong-min LIANG, Gui-min ZHU, Han-ning YANG, Wei-han CAO, Yong-ping LU. Application of Ultrasound-Targeted Microbubble Destruction with IL-6 Monoclonal Antibody in Rabbit Myocardial Ischemia-Reperfusion Injury[J]. Journal of Kunming Medical University, 2021, 42(2): 6-12. doi: 10.12259/j.issn.2095-610X.S20210222

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Application of Ultrasound-Targeted Microbubble Destruction with IL-6 Monoclonal Antibody in Rabbit Myocardial Ischemia-Reperfusion Injury

doi: 10.12259/j.issn.2095-610X.S20210222

1.
Dept. of Medical Ultrasound，The Fourth Affiliated Hospital of Kunming Medical University，The Second Hospital of Yunnan Province，Kunming Yunnan 650021
2.
Dept. of Medical Ultrasound，The First Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

Received Date: 2020-11-19
Publish Date: 2021-03-05

Abstract

Abstract

Objective To explore the application value of Ultrasound Targeted Microbubble Destruction （UTMD） with IL-6 monoclonal antibody combined with different ultrasonic irradiation intensity in myocardial ischemia-reperfusion injury （MI/RI）. Methods Ninety rabbits were randomly divided into 3 groups, including 15 rabbits in the thoracotomy control group （group A）, 15 rabbits in the thoracotomy control group （group B）, and 60 rabbits in the R/I group （group C, divided into T1-T4 periods, reperfusion for 30 min, 60 min, 120 min, and 180 min）. Each group was further divided into group U₀ （without ultrasonic irradiation）, group U₁ （0.5 w/cm² intensity）, group U₂ （0.75 w/cm² intensity）. An animal model of MI/RI was obtained by blocking the left anterior descending branch of the coronary artery for 30 min and then releasing it from reperfusion. The targeted contrast agent containing IL-6 was injected through ear vein, and then ultrasound irradiation was performed. QLAB10.5 software to analyze the video intensity of myocardium in reperfusion injury area before and after irradiation, and the ultrasonic video intensity difference （VID） was calculated. The content of IL-6 in the myocardium of each group before and after irradiation was detected by ELISA. Results The VID value of U₂ group was higher than that of U₁ group during T1-T4 （P < 0.05）. The values of U₂ and U₁ VID in theT1-T4 period were compared in pairs within the group, the difference was not statistically significant （P > 0.05）. ELISA analysis of rabbit myocardial tissue showed that the content of IL-6 in U₂ group was lower than that in U₁ group during T1-T4 period after ultrasonic irradiation （P < 0.05）. The difference of IL-6 content between U₁ group and U₂group during T1-T4 was compared in pairs within the group, the difference was not statistically significant （P > 0.05）. The difference of IL-6 content in myocardial tissue was positively correlated with the mean difference of ultrasonic video intensity （rU₁ = 0.745, rU₂ = 0.734）. Conclusion IL-6 monoclonal antibody microbubbles combined with UTMD can effectively alleviate MI/RI inflammatory response, in addition, under the premise of no damage to the myocardium, the earlier the intervention, the stronger the radiation intensity, the better the effect of reducing the inflammatory response.

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