Influence of DC-Chol Cationic Liposome Adjuvant on the Immune Effect of Tetravalent Influenza Vaccine

Yan-hong LIU; Xiang-yue ZHAO; Qing-xin XIE; Meng-di PU; Wei-dong LU

doi:10.12259/j.issn.2095-610X.S20210302

Volume 42 Issue 3

Apr. 2021

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Yan-hong LIU, Xiang-yue ZHAO, Qing-xin XIE, Meng-di PU, Wei-dong LU. Influence of DC-Chol Cationic Liposome Adjuvant on the Immune Effect of Tetravalent Influenza Vaccine[J]. Journal of Kunming Medical University, 2021, 42(3): 1-4. doi: 10.12259/j.issn.2095-610X.S20210302

Citation:

Yan-hong LIU, Xiang-yue ZHAO, Qing-xin XIE, Meng-di PU, Wei-dong LU. Influence of DC-Chol Cationic Liposome Adjuvant on the Immune Effect of Tetravalent Influenza Vaccine[J]. Journal of Kunming Medical University, 2021, 42(3): 1-4. doi: 10.12259/j.issn.2095-610X.S20210302

Citation:

Yan-hong LIU, Xiang-yue ZHAO, Qing-xin XIE, Meng-di PU, Wei-dong LU. Influence of DC-Chol Cationic Liposome Adjuvant on the Immune Effect of Tetravalent Influenza Vaccine[J]. Journal of Kunming Medical University, 2021, 42(3): 1-4. doi: 10.12259/j.issn.2095-610X.S20210302

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Influence of DC-Chol Cationic Liposome Adjuvant on the Immune Effect of Tetravalent Influenza Vaccine

doi: 10.12259/j.issn.2095-610X.S20210302

School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2020-12-10
Publish Date: 2021-04-09

Abstract

Abstract

Objective To study the immunogenicity of the tetravalent influenza vaccine modified by cationic liposome DC-Chol. Methods DC-Chol liposomes were prepared by thin film dispersion-freeze-thaw- freeze-drying method, and the encapsulation rate was determined. The mice were divided into seven groups, DC-Chol liposome vaccine group （250 µg/mouse, 500 µg/mouse, 750 µg/mouse, 900 µg/mouse）, PBS group, vaccine stock solution group, neutral liposome group （n = 3）Determine the optimal dose of DC-Chol liposomes by MTT method. The mice were divided into 4 groups, DC-Chol liposome group, vaccine stock solution group, neutral liposome group and PBS control group. Mice were immunized through the peritoneum and sacrificed on the 7, 14, and 28 days. The proliferation of mouse lymphocytes was detected by MTT method and T lymphocyte surface labeling experiment to study its immunogenicity. Results The MTT experiment showed that the optimal dose of DC-Chol modified liposomes was 500 μg/rat （P < 0.05）; T lymphocyte surface labeling experiments showed that DC-Chol cationic liposomes significantly increased the number of splenic lymphocytes （P < 0.05）. Conclusion Compared with neutral liposomes, DC-Chol cationic liposomes as an adjuvant of tetrovalent influenza vaccine has good immune enhancement effect.
- Liposome,
- DC-Chol,
- Influenza vaccine,
- Cellular immunity

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References(18)

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