Antibacterial Effect of Slightly Acidic Electrolyzed Water on <i>Enterococcus faecalis</i> Biofilm in Root Canal in Vitro

Qian JIANG; Yu SUN; Li-jun HUO; Ya-yan LEI; Luo-wei PEI

doi:10.12259/j.issn.2095-610X.S20210303

Volume 42 Issue 3

Apr. 2021

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2021 > 42(3): 5-9

Qian JIANG, Yu SUN, Li-jun HUO, Ya-yan LEI, Luo-wei PEI. Antibacterial Effect of Slightly Acidic Electrolyzed Water on Enterococcus faecalis Biofilm in Root Canal in Vitro[J]. Journal of Kunming Medical University, 2021, 42(3): 5-9. doi: 10.12259/j.issn.2095-610X.S20210303

Citation:

Qian JIANG, Yu SUN, Li-jun HUO, Ya-yan LEI, Luo-wei PEI. Antibacterial Effect of Slightly Acidic Electrolyzed Water on Enterococcus faecalis Biofilm in Root Canal in Vitro[J]. Journal of Kunming Medical University, 2021, 42(3): 5-9. doi: 10.12259/j.issn.2095-610X.S20210303

Citation:

PDF( 773 KB)

Antibacterial Effect of Slightly Acidic Electrolyzed Water on Enterococcus faecalis Biofilm in Root Canal in Vitro

doi: 10.12259/j.issn.2095-610X.S20210303

1.
Dept. of Dental Endodontics，The Affiliated Stomatology Hospital of Kunming Medical University，Kunming，Yunnan 650100
2.
Shanghai Fuqiangwang Hygiene Products Co.，Ltd.，Shanghai 200000，China

Received Date: 2020-12-14
Publish Date: 2021-04-09

Abstract

Abstract

Objective To study the antibacterial effect of slightly acidic electrolyzed water （SAEW） as root canal irrigation on Enterococus faecalis （E. faecalis） biofilm in root canal in vitro. Methods E. faecalis biofilms were cultured in the root canals of isolated teeth and washed with SAEW at concentrations of 50 mg/L and 60 mg/L for 1 min, 3 mins, and 5 mins, respectively, the 1% NaClO solutions was used as positive controls. The field emission scanning electron microscope （FE-SEM） was used to observe E. faecalis biofilm morphological changes, the confocal laser scanning microscope （CLSM） combined with Live/Dead fluorescence staining was used to observe the E. faecalis biofilms and analyze the proportion of dead bacteria in the biofilms. Results FE-SEM observation: 50 mg/L SAEW, 60 mg/L SAEW, 1% NaCl Osolutions with increasing washing time, E.faecalis in the biofilm decreased, the surface of the was rough, and the cells shrank. Confocal laser scanning microscope （CLSM） combined with Live/Dead fluorescence staining observation: 50 mg/L SAEW, 60 mg/L SAEW, 1% NaClO solutions with increasing washing time, the red fluorescence intensity gradually increased, and the proportion of dead bacteria gradually increased （P < 0.05）, the sterilization ratio of 1% NaClO to E. faecalis at the time point of 1 min, 3 min, and 5 min was significantly higher than 50 mg/L SAEW, 60 mg/L SAEW （P < 0.05）. Conclusion Slightly acidic electrolyzed water has a certain antibacterial effect on strains in E. faecalis biofilm, but the effect is lower than 1% NaCIO solutions.
- Slightly acidic electrolyzed water,
- Enterococus faecalis,
- Biofilm,
- Sodium hypochlorite,
- Antibacteria

FullText(HTML)

References(14)

References

[1]	Stuart C H,Schwartz S A,Beeson T J,et al. Enterococcus faecalis:Its role in root canal treatment failure and current concepts in retreatment[J]. Journal of Endodontics,2006,32(2):93-98. doi: 10.1016/j.joen.2005.10.049
[2]	Ngnitcho P F K,Tango C N,Khan I,et al. The applicability of Weibull model for the kinetics inactivation of Listeria monocytogenes and Escherichia coli O157:H7 on soybean sprouts submitted to chemical sanitizers in combination with ultrasound at mild temperatures[J]. LWT,2018,91(1):573-579.
[3]	Zhang C L,Chen X,Xia X D,et al. Viability assay of E. coli O157:H7 treated with electrolyzed oxidizing water using flow cytometry[J]. Food Control,2018,88:47-53. doi: 10.1016/j.foodcont.2017.12.029
[4]	Hussain M S,Tango C N,Oh D H. Inactivation kinetics of slightly acidic electrolyzed water combined with benzalkonium chloride and mild heat treatment on vegetative cells,spores,and biofilms of Bacillus cereus[J]. Food Research International,2019,116:157-167. doi: 10.1016/j.foodres.2018.08.003
[5]	向盈盈,于鸿滨,周静,等. 致病性粪肠球菌YN771的分离鉴定及其生物学特性[J]. 昆明医科大学学报,2020,41(5):46-51. doi: 10.3969/j.issn.1003-4706.2020.05.009
[6]	Haapasalo M,Orstavik D. In vitro infection and of dentinal tubules[J]. Journal of Dental Research,1987,66(8):1375-1379. doi: 10.1177/00220345870660081801
[7]	Bryce G,O'donnell D,Ready D,et al. Contemporary root canal irrigants are able to disrupt and eradicate single- and dual-species biofilms[J]. Journal of Endodontics,2009,35(9):1243-1248. doi: 10.1016/j.joen.2009.05.034
[8]	Hao X,Shen Z,Wang J,et al. In vitro inactivation of porcine reproductive and respiratory syndrome virus and pseudorabies virus by slightly acidic electrolyzed water[J]. The Veterinary Journal,2013,197(2):297-301. doi: 10.1016/j.tvjl.2013.02.007
[9]	Dukan S,Belkin S,Touati D. Reactive oxygen species are partially involved in the bacteriocidal action of hypochlorous acid[J]. Archives of Biochemistry and Biophysics,1999,367(2):311-316. doi: 10.1006/abbi.1999.1265
[10]	Siqueira Jr J F,Rôças I N,Santos S R,et al. Efficacy of instrumentation techniques and irrigation regimens in reducing the bacterial population within root canals[J]. Journal of Endodontics,2002,28(3):181-184. doi: 10.1097/00004770-200203000-00009
[11]	Nagamatsu Y,Chen K K,Tajima K,et al. Durability of bactericidal activity in electrolyzed neutral water by storage[J]. Dental Materials Journal,2002,21(2):93-104. doi: 10.4012/dmj.21.93
[12]	Pashley E L,Birdsong N L,Bowman K,et al. Cytotoxic effects of naocl on vital tissue[J]. Journal of Endodontics,1985,11(12):525-528. doi: 10.1016/S0099-2399(85)80197-7
[13]	Park G W,Boston D M,Kase J A,et al. Evaluation of liquid- and fog-based application of Sterilox hypochlorous acid solution for surface inactivation of human norovirus[J]. Applied and Environmental Microbiology,2007,73(14):4463-4468. doi: 10.1128/AEM.02839-06
[14]	Solovyeva A M,Dummer P M H. Cleaning effectiveness of root canal irrigation with electrochemically activated anolyte and catholyte solutions:a pilot study[J]. International Endodontic Journal,2000,33(6):494-504. doi: 10.1046/j.1365-2591.2000.00342.x

Relative Articles(20)

Relative Articles

[1]	Yang LYU, Guiding LI, Shaowen LU, Binbing YU, Qiuju ZHAI, Yi PENG. Disinfecting Effect of Three Disinfectants on the Ultrasonic Scaling Unit Waterline. Journal of Kunming Medical University, 2024, 45(9): 180-188. doi: 10.12259/j.issn.2095-610X.S20240928
[2]	Danyan SU, Wentian TANG, Jinxu YANG, Hua LIU, Bangsheng LI, Yingding ZHAO, Yunchao HUANG. Research Progress of Indole in E. coli Biofilms on the Surface of Biomaterials. Journal of Kunming Medical University, 2023, 44(12): 184-190. doi: 10.12259/j.issn.2095-610X.S20231229
[3]	Xueting YANG, Huifeng ZHANG. Evaluation of Drug Utilization in the Infected Patients with ESBLs Produced by Enterobacteriaceaein. Journal of Kunming Medical University, 2022, 43(6): 74-78. doi: 10.12259/j.issn.2095-610X.S20220621
[4]	Tongxin YANG, Guang WANG, Rui XU, Siwei SU, Kewei FANG, Jianhe LIU, Jiongming LI. Effect of Cathelicidin LL-37 on Transmembrane Barrier Function of Urothelial Cells. Journal of Kunming Medical University, 2022, 43(8): 34-40. doi: 10.12259/j.issn.2095-610X.S20220806
[5]	Zhenghong YANG, Mingjie NING, Daqian HE, Mengzhe YANG, Yongping HUANG, Yunchao HUANG. Effect of Surface Topography on Staphylococcus Epidermidis Biofilm Formation by Different 3D Printing Thickness of Biomaterials. Journal of Kunming Medical University, 2022, 43(2): 12-17. doi: 10.12259/j.issn.2095-610X.S20220228
[6]	Li-han YANG, Zhi-bi ZHANG, Hui YANG, Jiang-lan YANG, Dan MAO, Xiao-li YANG, Chao-yu LUO, Yuan ZHANG, Rong-ping ZHANG. Comparative Study on Anti-inflammatory and Antimicrobial Effects for Two Kinds of the Resin Gel. Journal of Kunming Medical University, 2021, 42(1): 6-11. doi: 10.12259/j.issn.2095-610X.S20210134
[7]	Jing ZHOU, Li-jun HUO, Ya-yan LEI, Hong-bing HE. Advances in Extracellular Polymeric Substances in Biofilm. Journal of Kunming Medical University, 2021, 42(4): 150-154. doi: 10.12259/j.issn.2095-610X.S20210429
[8]	Jie-chen QIN, Xiao-fei ZUO, Cheng ZOU, Cheng-jie TUO, Ke-tao ZHANG, Xiao-mei ZHANG, Qing ZHAO. Antimicrobial and Antitumor Zingiberaceae Diterpene Derivatives Prepared by Structure Modification. Journal of Kunming Medical University, 2021, 42(5): 1-5. doi: 10.12259/j.issn.2095-610X.S20210501
[9]	Xiang Ying Ying , Yu Hong Bin , Zhou Jing , Yang Xiang Hong , Song Fei , Wei Yun Lin , Ji Xiu Ling . . Journal of Kunming Medical University, 2020, 41(05): 46-51.
[10]	Jiang Yu , Qian Wei Min , Ye Ling , Ning Jing , Li Jian Min . . Journal of Kunming Medical University, 2019, 40(04): 57-60.
[11]	Zhang An Xing , Wu Jing , Sun Yang , Miao Ying Lei . . Journal of Kunming Medical University, 2018, 39(09): 128-132.
[12]	Jiang Juan Na , Jie Bao Sheng , Wu Yun Ding , Zhou Tian . Effect of DAPC on Adhesion of Streptococcus Mutans. Journal of Kunming Medical University, 2018, 39(03): 55-58.
[13]	Zhang Ya Juan , Pan Hong , Du Jia Hui , Wu Jie , Qiu Xiu Qin , Liu Song Bai , Jia Bei Bei . The Effect of Cinnamaldehyde and Vancomycin on Biofilm Inhibition of Methicillin-Resistant Staphylococcus Aureus. Journal of Kunming Medical University, 2018, 39(04): 52-55.
[14]	Zhu Yan Ping . . Journal of Kunming Medical University,
[15]	Sun Hui Feng . . Journal of Kunming Medical University,
[16]	Qin Ya Hui . . Journal of Kunming Medical University,
[17]	Sun Jian Ming . . Journal of Kunming Medical University,
[18]	Hu Hai Yan . . Journal of Kunming Medical University,
[19]	Gao Zhen Hua . . Journal of Kunming Medical University,
[20]	Li Si Man . The Antimicrobial Activity of Snake Venom Antimicrobial Peptide Cathelicidin on E. coli. Journal of Kunming Medical University,