miR-490-3p Suppresses EMT of SW1990 Pancreatic Cancer Cells

Jing-hui LI; Ming ZHU; Hai QU; Qiu-yu LI; Yu-juan WU; He-ying YANG; Yu-zhu WANG; Yan-ping LI

doi:10.12259/j.issn.2095-610X.S20210305

Volume 42 Issue 3

Apr. 2021

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Article Navigation > Journal of Kunming Medical University > 2021 > 42(3): 10-17

Jing-hui LI, Ming ZHU, Hai QU, Qiu-yu LI, Yu-juan WU, He-ying YANG, Yu-zhu WANG, Yan-ping LI. miR-490-3p Suppresses EMT of SW1990 Pancreatic Cancer Cells[J]. Journal of Kunming Medical University, 2021, 42(3): 10-17. doi: 10.12259/j.issn.2095-610X.S20210305

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Jing-hui LI, Ming ZHU, Hai QU, Qiu-yu LI, Yu-juan WU, He-ying YANG, Yu-zhu WANG, Yan-ping LI. miR-490-3p Suppresses EMT of SW1990 Pancreatic Cancer Cells[J]. Journal of Kunming Medical University, 2021, 42(3): 10-17. doi: 10.12259/j.issn.2095-610X.S20210305

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miR-490-3p Suppresses EMT of SW1990 Pancreatic Cancer Cells

doi: 10.12259/j.issn.2095-610X.S20210305

1.
Dept. of Critical Care Medicine
2.
Dept. of Neurology，Yan’an Hospital of Kunming，Kunming Yunnan 650051，China

Received Date: 2020-12-15
Publish Date: 2021-04-09

Abstract

Abstract

Objective To elucidate the role of miR-490-3p in regulating HMGA2 expression and affecting epithelial-mesenchymal transition （EMT） of SW1990 cells. Methods miR-490-3p expression was measured in HPDE and SW1990 cell lines by Real-time PCR. SW1990 cell lines were cultured in vitro and divided into four groups, including inhibitor-Negative control （inhibitor-NC）, miR-490-3p inhibitor, mimic- Negative control （mimic-NC） and miR-490-3p mimic group, and the transfection efficiency was verified by RT-qPCR. After 48 h of transfection, CCK8 assay, wound healing assay, Transwell invasion assay andflow cytometric were used to detect the effects of malignant biological characteristics such as proliferation, migration, invasion and apoptosis of SW1990 cell linerespectively. Western blot was used to detect the expression of E-cadherin and N-cadherin. Meanwhile, database predicted the target genes of miR-490-3p, and dual luciferase assay confirmed the targeted relationship between miR-490-3p and HMGA2, western blot was used to detect the expression of HMGA2. Results 1） The expression of miR-490-3p was downregulated in SW1990 cells （P = 0.215）. 2） Overexpression of miR-490-3p significantly inhibited the proliferation （P < 0.0001）, migration （P < 0.0001） and invasion （P < 0.0001） of SW1990 cell line and prompted apoptosis （P < 0.0001）; In contrast, downregulation of miR-490-3p significantly promoted the proliferation （P < 0.0001）, migration （P < 0.0001） and invasion （P < 0.0001） of SW1990 cell line and inhibited apoptosis （P < 0.0001）. 3） Upregulation of miR-490-3p significantly decreased the expression of N-cadherin （P < 0.0001） and increased E-cadherin （P < 0.0001） expression in SW1990 cell line. In contrast, downregulation of miR-490-3p significantly upregulated （P < 0.0001） the expression of N-cadherin, decreased E-cadherin （P < 0.0001） expression. 4） HMGA2 was a target gene of miR-490-3p. Conclusion These findings indicate that miR-490-3p acts as a tumor suppressor during the process of EMT through targeting HMGA2, suggesting miR-490-3p is a potential new diagnostic and therapeutic target for the treatment of pancreatic cancer.
- Pancreatic cancer,
- EMT,
- miR-490-3p,
- HMGA2

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